US10251400B2 - Mixtures comprising a Bacillus strain and a pesticide - Google Patents

Mixtures comprising a Bacillus strain and a pesticide Download PDF

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US10251400B2
US10251400B2 US15/313,229 US201515313229A US10251400B2 US 10251400 B2 US10251400 B2 US 10251400B2 US 201515313229 A US201515313229 A US 201515313229A US 10251400 B2 US10251400 B2 US 10251400B2
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nrrl
plant
component
spp
amyloliquefaciens
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US20170188584A1 (en
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Thorsten Jabs
Nenad FILAJDIC
Giridhar RANUVA
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BASF SE
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
    • A01N63/22—Bacillus
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
    • A01N37/50—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids the nitrogen atom being doubly bound to the carbon skeleton
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
    • A01N43/40—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom six-membered rings
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
    • A01N43/54—1,3-Diazines; Hydrogenated 1,3-diazines
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
    • A01N43/56—1,2-Diazoles; Hydrogenated 1,2-diazoles
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/64—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with three nitrogen atoms as the only ring hetero atoms
    • A01N43/647—Triazoles; Hydrogenated triazoles
    • A01N43/653—1,2,4-Triazoles; Hydrogenated 1,2,4-triazoles
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/72—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
    • A01N43/80—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms five-membered rings with one nitrogen atom and either one oxygen atom or one sulfur atom in positions 1,2
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
    • A01N47/02—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having no bond to a nitrogen atom
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
    • A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
    • A01N47/10—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
    • A01N47/12—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing a —O—CO—N< group, or a thio analogue thereof, neither directly attached to a ring nor the nitrogen atom being a member of a heterocyclic ring
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
    • A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
    • A01N47/10—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
    • A01N47/24—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing the groups, or; Thio analogues thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N51/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds having the sequences of atoms O—N—S, X—O—S, N—N—S, O—N—N or O-halogen, regardless of the number of bonds each atom has and with no atom of these sequences forming part of a heterocyclic ring
    • A01N63/02—
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/10—Animals; Substances produced thereby or obtained therefrom
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N2300/00—Combinations or mixtures of active ingredients covered by classes A01N27/00 - A01N65/48 with other active or formulation relevant ingredients, e.g. specific carrier materials or surfactants, covered by classes A01N25/00 - A01N65/48
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07—Bacillus
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
    • Y02A50/356—

Definitions

  • the present invention relates to mixtures comprising, as active components the Bacillus strains AP-136, AP-188, APP-218, AP-219, AP-295, AP-209 and/or AP-217 and a pesticide.
  • Bacillus amyloliquefaciens AP-136, AP-188, AP-218, AP-219, AP-295 , Bacillus mojavensis AP-209 and Bacillus solisalsi AP-217 are known from US 2012/0149571, the content of which is incorporated by reference in its entirety. They have been described therein specifically as inoculants for promoting the plant defense response against herbivorous insects.
  • the strain B. amyloliquefaciens AP-136 has been deposited with the United States Department of Agriculture (USDA) on Nov. 5, 2009 under accession number (acc. no.) NRRL B-50330 and on Dec. 2, 2011 under acc. no. NRRL B-50614.
  • the strain B. amyloliquefaciens AP-188 has been deposited with USDA on Nov. 5, 2009 under acc. no. NRRL B-50331 and on Dec. 2, 2011 under acc. no. NRRL B-50615.
  • the strain B. amyloliquefaciens AP-218 has been deposited with USDA on Dec. 2, 2011 under acc. no. NRRL B-50618.
  • amyloliquefaciens AP-219 has been deposited with USDA on Nov. 5, 2009 under acc. no. NRRL B-50332 and on Dec. 2, 2011 under acc. no. NRRL B-50619.
  • the strain B. amyloliquefaciens AP-295 has been deposited with USDA on Nov. 5, 2009 under acc. no. NRRL B-50333 and on Dec. 2, 2011 under acc. no. NRRL B-50620.
  • the strain B. mojavensis AP-209 has been deposited with USDA on Dec. 2, 2011 under acc. no. NRRL B-50616.
  • the strain B. solisalsi AP-217 has been deposited with USDA on Dec. 2, 2011 under acc. no. NRRL B-50617.
  • a pesticide is generally a chemical or biological agent (such as a virus, bacterium, antimicrobial or disinfectant) that through its effect deters, incapacitates, kills or otherwise discourages pests.
  • Target pests can include insects, plant pathogens, weeds, mollusks, birds, mammals, fish, nematodes (roundworms), and microbes that destroy property, cause nuisance, spread disease or are vectors for disease.
  • Biopesticides have been defined as a form of pesticides based on micro-organisms (bacteria, fungi, viruses, nematodes, etc.) or natural products (compounds, such as metabolites, proteins, or extracts from biological or other natural sources) (U.S. Environmental Protection Agency: http://www.epa.gov/pesticides/biopesticides/). Biopesticides fall into two major classes, microbial and biochemical pesticides:
  • Microbial pesticides consist of bacteria, fungi or viruses (and often include the metabolites that bacteria and fungi produce).
  • Biochemical pesticides are naturally occurring substances or or structurally-similar and functionally identical to a naturally-occurring substance and extracts from biological sources that control pests or provide other crop protection uses as defined below, but have non-toxic mode of actions and are relatively non-toxic to mammals.
  • Biopesticides for use against crop diseases have already established themselves on a variety of crops. For example, biopesticides already play an important role in controlling downy mildew diseases. Their benefits include: a 0-Day Pre-Harvest Interval, the ability to use under moderate to severe disease pressure, and the ability to use in mixture or in a rotational program with other registered pesticides.
  • biopesticides under certain conditions can also have disadvantages such as high specificity: which may require an exact identification of the pest/pathogen and the use of multiple products to be used, slow speed of action (thus making them unsuitable if a pest outbreak is an immediate threat to a crop), variable efficacy due to the influences of various biotic and abiotic factors (since biopesticides are usually living organisms, which bring about pest/pathogen control by multiplying within the target insect pest/pathogen) and resistance development.
  • It is an object of the present invention overcome the abovementioned disadvantages and to provide, with a view to effective resistance management and effective control of phytopathogenic harmful fungi, insects or other pests or to effective plant growth regulation, at application rates which are as low as possible, compositions which, at a reduced total amount of active compounds applied, have improved activity against the harmful fungi, insects or other pests or improved plant growth regulating activity (synergistic mixtures) and a broadened activity spectrum, in particular for certain indications.
  • the invention can also result in an advantageous behavior during formulation or during use, for example during grinding, sieving, emulsifying, dissolving or dispensing; improved storage stability and light stability, advantageous residue formation, improved toxicological or ecotoxicological behaviour, improved properties of the plant, for example better growth, increased harvest yields, a better developed root system, a larger leaf area, greener leaves, stronger shoots, less seed required, lower phytotoxicity, mobilization of the defense system of the plant, improved compatibility with plants.
  • the present invention relates to mixtures comprising, as active components
  • the mixtures comprise at least one microorganism of the genus Bacillus seleted from B. amyloliquefaciens AP-136 (NRRL B-50330; NRRL B-50614), B. amyloliquefaciens AP-188 (NRRL B-50331; NRRL B-50615), B. amyloliquefaciens AP-218 (NRRL B-50618), B. amyloliquefaciens AP-219 (NRRL B-50332, NRRL B-50619), B. amyloliquefaciens AP-295 (NRRL B-50333, NRRL B-50620) B.
  • mojavensis AP-209 (NRRL B-50616), and B. solisalsi AP-217 (NRRL B-50617) (which are herein also referred to as AP-136, AP-188, AP-218, AP-219, AP-295, AP-209 and AP-217, respectively); and at least one pesticide II in a synergistically effective amount.
  • the invention also relates to a method for controlling phytopathogenic harmful fungi using mixtures of at least one microorganism selected from AP-136, AP-188, AP-218, AP-219, AP-295, AP-209 and AP-217 and and at least one pesticide II and to the use of microorganisms I and pesticides II for preparing such mixtures, and to compositions comprising these mixtures and seed comprising these mixtures or coated with this this mixture.
  • the time between both applications may vary e.g. between 2 hours and 7 days. Also a broader range is possible ranging from 0.25 hour to 30 days, preferably from 0.5 hour to 14 days, particularly from 1 hour to 7 days or from 1.5 hours to 5 days, even more preferred from 2 hours to 1 day.
  • the at least one microorganism I is applied as last treatment.
  • Component 1) in the mixtures embraces not only the isolated, pure cultures of at least one microorganism I as defined herein, but also a its cell-free extract having pesticidal activity, preferably a ketone-based extract, its suspensions in a whole broth culture or as a metabolite-containing supernatant or a purified metabolite obtained from a whole broth culture of the microorganism or microorganism strain.
  • a cell-free extract having pesticidal activity preferably a ketone-based extract, its suspensions in a whole broth culture or as a metabolite-containing supernatant or a purified metabolite obtained from a whole broth culture of the microorganism or microorganism strain.
  • whole culture broth refers to a liquid culture of a microorganism containing vegetative cells and/or spores suspended in the culture medium and optionally metabolites produced by the respective microorganism.
  • strain refers to isolate or a group of isolates exhibiting phenotypic and/or genotypic traits belonging to the same lineage, distinct from those of other isolates or strains of the same species.
  • component 1) is at least one microorganism selected from AP-136, AP-188, AP-218, AP-219, AP-295, AP-209 and AP-217, and a cell-free extract of the aforementioned strains.
  • component 1) is at least one microorganism selected from AP-136, AP-188, AP-218, AP-219, AP-295, AP-209 and AP-217.
  • component 1) is at least one microorganism selected from AP-136, AP-188, AP-218, AP-219, AP-295, AP-209 and AP-217 in a whole browth.
  • component 1) is at least one microorganism selected from AP-136, AP-188, AP-218, AP-219, AP-295, AP-209 and AP-217 in a dormant form.
  • component 1) is at least one microorganism selected from AP-136, AP-188, AP-218, AP-219, AP-295, AP-209 and AP-217 in the form of spores.
  • the microorganisms AP-136, AP-188, AP-218, AP-219, AP-295, AP-209 and AP-217 can be cultivated e.g. using Potato dextrose agar (PDA) under aerobvic growth conditions at about 28° C. In large liquid cultures, aeriation may be necessary.
  • PDA Potato dextrose agar
  • the bacterial cells can be washed and concentrated (e.g. by centrifugation at room temperature for about 15 min at 7000 ⁇ g).
  • bacterial cells, preferably spores were suspended in a suitable dry carrier (e.g. clay).
  • cells preferably spores
  • a suitable liquid carrier e.g. water-based
  • the spore density number of spores per mL can be determined by identifying the number of colony-forming units (CFU) on agar medium e.g. potato dextrose agar after incubation for several days at 28° C.
  • CFU colony-forming units
  • the microorganism I are generally active in temperatures between 5° C. and 50° C., preferably between 15° C. and 35° C.
  • component 1) is at least one microorganism I selected from Bacillus amyloliquefaciens AP-136 (NRRL B-50330; NRRL B-50614), B. amyloliquefaciens AP-188 (NRRL B-50331; NRRL B-50615), B. amyloliquefaciens AP-218 (NRRL B-50618), B. amyloliquefaciens AP-219 (NRRL B-50332, NRRL B-50619), and B. amyloliquefaciens AP-295 (NRRL B-50333, NRRL B-50620); in particular B. amyloliquefaciens AP-188.
  • component 1) is at least one microorganism I selected from B. mojavensis AP-209 (NRRL B-50616) and B. solisalsi AP-217 (NRRL B-50617).
  • component 2) is selected from Bradyrhizobium japonicum, B. elkanii, Bradyrhizobium spp., Bradyrhizobium sp. ( Arachis ), Bradyrhizobium sp. ( Vigna ), B. liaoningense, B. lupine; Azospirillum brasilense, A. amazonense, A. lipoferum, A. irakense, A. halopraeferens; Delftia acidovorans, Glomus intraradices; Mesorhizobium spp., Mesorhizobium ciceri, M. huakii, M.
  • Rhizobium leguminosarum bv. phaseoli R. leguminosarum bv. trifolii, R. leguminosarum bv. viciae, R. tropici, Sinorhizobium mellloti; Bacillus amyloliquefaciens, B. amyloliquefaciens ssp. plantarum, B. firmus, B. pumilus, B. subtilis, B. simplex, B. megaterium, B. altitudinis, B. mojavensis, B. mycoides, B.
  • component 2) is selected from Bradyrhizobium japonicum, B. elkanii, Azospinflum brasilense; Bacillus amyloliquefaciens, B. amyloliquefaciens ssp. plantarum, B. firmus, B. pumilus, B. subtilis, B. simplex, B.
  • the mixtures comprise as component 2) at least one pesticide II selected from Bradyrhizobium japonicum, B. elkanii, Azospirillum brasilense ; cis-jasmone, methyl jasmonate and harpin protein.
  • component 2) is selected from Bacillus firmus, B. pumilus, Burkholderia spp., Muscodor albus and Paecilomyces lilacinus . These mixture are especially effective for control of nematodes.
  • the mixture comprise in addition to component 1) and component 2) a further active component 3) which is selected from methyl jasmonate, cis-jasmone and harpin, provided that component 2) is different from component 3).
  • biopesticides from group L) of pesticides II, their preparation and their pesticidal activity e. g. against harmful fungi or insects are known (e-Pesticide Manual V 5.2 (ISBN 978 1 901396 85 0) (2008-2011); http://www.epa.gov/opp00001/biopesticides/, see product lists therein; http://www.omri.org/omri-lists, see lists therein; Bio-Pesticides Database BPDB http://sitem.herts.ac.uk/aeru/bpdb/, see A to Z link therein).
  • the biopesticides from group L1) and/or L2) may also have insecticidal, acaricidal, molluscidal, pheromone, nematicidal, plant stress reducing, plant growth regulator, plant growth promoting and/or yield enhancing activity.
  • the biopesticides from group L3) and/or L4) may also have fungicidal, bactericidal, viricidal, plant defense activator, plant stress reducing, plant growth regulator, plant growth promoting and/or yield enhancing activity.
  • the biopesticides from group L5) and/or L6) may also have fungicidal, bactericidal, viricidal, plant defense activator, insecticidal, acaricidal, molluscidal, pheromone and/or nematicidal activity.
  • biopesticides have been deposited under deposition numbers mentioned herein (the prefices refer to the acronym of the respective culture collection), are referred to in literature, registered and/or are commercially available: aluminium silicate (ScreenTM Duo from Certis LLC, USA), Agrobacterium radiobacter K1026 (e. g. NoGall® from BASF Agricultural Specialties Pty Ltd, Australia), A. radiobacter K84 (Nature 280, 697-699, 1979; e. g. GallTroll® from AG Biochem, Inc., C, USA), Ampelomyces quisqualis M-10 (e. g. AQ 10® from Intrachem Bio GmbH & Co.
  • A. brasilense Sp245 (BR 11005; e. g. in GELFIX Gram ⁇ neas from BASF Agricultural Specialties Ltd., Brazil), A. brasilense strains Ab-V5 and Ab-V6 (e. g. in AzoMax from Novozymes BioAg Produtos papra Agricultura Ltda., Quattro Barras, Brazil or SimbioseMa ⁇ z® from Simbiose-Agro, Cruz Alta, RS, Brazil; Plant Soil 331, 413-425, 2010), A.
  • lipoferum BR 11646 (Sp31) (Proc. 9 th Int. and 1 st Latin American PGPR meeting, Quimara, Medell ⁇ n, Colombia 2012, p. 60), Bacillus altitudinis 41KF2b (DSM 21631; Int. J. Syst. Evol. Microbiol.
  • Bacillus amyloliquefaciens strains AP-136 (NRRL B-50614 and B-50330), AP-188 (NRRL B-50615 and B-50331), AP-218 (NRRL B-50618), AP-219 (NRRL B-50619 and B-50332), and AP-295 (NRRL B-50620 and B-50333) all known from U.S. Pat. No. 8,445,255; B. amyloliquefaciens IT-45 (CNCM 1-3800) (e. g. Rhizocell C from ITHEC, France), B. amyloliquefaciens IN937a (J. Microbiol. Biotechnol.
  • B. amyloliquefaciens spp. plantarum D747 US 20130236522 A1; FERM BP-8234; e. g. Double NickelTM 55 WDG or Double NickelTM LC from Certis LLC, USA
  • B. amyloliquefaciens spp. plantarum SB3615vPPI being a phage-resistant variant of FZB24 (MRRL B-50349; US 2011/023045 A1; from Novozyme Biologicals, Inc., USA)
  • B. amyloliquefaciens ssp. plantarum FZB42 isolated from plant pathogen-infested soil of a sugar beet field in Brandenburg, Germany (J. Plant Dis. Prot. 105, 181-197, 1998; DSM 23117; e. g. RhizoVital® 42 from AbiTEP GmbH, Berlin, Germany), B.
  • amyloliquefaciens ssp. plantarum GB03 also called GBO3; ATCC SD-1397; Phytopathol. 86(11), S36, 1996; e. g. Kodiak® or BioYield® from Gustafson, Inc., USA; or Companion® from Growth Products, Ltd., White Plains, N.Y. 10603, USA
  • B. amyloliquefaciens ssp. plantarum MB1600 also referred to as 1430 (NRRL B-50595; Int. J. Microbiol. Res. 3(2) (2011), 120-130; US 2012/0149571 A1; e. g.
  • B. amyloliquefaciens spp. plantarum TJ1000 also called 1BE; CA 2471555 A1; ATCC BAA-390; e. g. QuickRootsTM from TJ Technologies, Watertown, S. Dak., USA
  • B. cereus CNCM 1-1562 U.S. Pat. No. 6,406,690
  • B. chitinosporus AQ746 isolated from roots in Saskatchewan, Canada NRRL B-21618; U.S. Pat. No. 5,733,544; AgraQuest now Bayer CropScience LP, USA), B.
  • CNCM 1-1582 (WO 2009/126473, WO 2009/124707, U.S. Pat. No. 6,406,690; e. g. Votivo® from Bayer CropScience LP, USA), B. megaterium strains H491 (NRRL B-50769), M018 (NRRL B-50770) and J142 (NRRL B-50771) all known from US 2014/0051571 A1 from Marrone BioInnovations, Inc., USA; B. mojavensis AP-209 (NRRL B-50616; U.S. Pat. No. 8,445,255), B. mycoides AQ726 (NRRL B-21664; U.S. Pat. No.
  • B. mycoides strain J e.g. BmJ WG from Certis, USA against potato virus Y
  • B. pumilus GB34 ATCC 700814; e. g. YieldShield® from Gustafson LLC, TX, USA
  • B. pumilus GHA 180 isolated from apple tree rhizosphere in Mexico IDAC 260707-01; e. g. in PRO-MIX® BX from Premier Horticulture, 1, avenue Premier, Rivie're-du-Loup, Quebec, Canada G5R6C1
  • B. pumilus KFP9F NRRL B-50754; WO 2014/029697; e. g.
  • B. pumilus INR-7 otherwise referred to as BU-F22 and BU-F33
  • B. pumilus INR-7 otherwise referred to as BU-F22 and BU-F33
  • B. pumilus QST 2808 NRRL B-30087; e. g. Sonata® or Ballad® Plus from AgraQuest Inc., USA
  • B. solisalsi AP-217 NRRL B-50617; U.S. Pat. No. 8,445,255
  • subtilis CX-9060 Federal Register 77(7), 1633-1637; by Certis U.S.A., L.L.C.
  • B. subtilis FB17 also called UD 1022 or UD10-22 isolated from red beet roots in North America (ATCC PTA-11857; System. Appl. Microbiol. 27, 372-379, 2004; US 2010/0260735; WO 2011/109395); B. subtilis GB07 (Phytopathol. 86(11), S36, 1996; Epic® from Gustafson, Inc., USA), B. subtilis QST-713 isolated from a California peach orchard in 1995 (NRRL B-21661; e. g.
  • B. thuringiensis ssp. aizawai ABTS-1857 also called ABG-6346; ATCC SD-1372; e. g. XenTari® from BioFa AG, Munsingen, Germany
  • Bacillus t . ssp. israelensis AM65-52 of Serotype H-14 ATCC SD-1276; e. g.
  • ssp. tenebrionis DSM 2803 of Serotype H 8a, 8b (identical to NRRL B-15939; EP 0 585 215 B1; Mycogen Corp.), B. t . ssp. tenebrionis NB-125 (also referred to as SAN 418 I or ABG-6479; EP 0 585 215 B1; DSM 5526; former production strain of Novo-Nordisk), B. t . ssp. tenebrionis NB-176 (or NB-176-1; a gamma-irridated, induced high-yielding mutant of strain NB-125; EP 585 215 B1; DSM 5480; e.
  • brongniartii for control of cockchafer J. Appl. Microbiol. 100(5),1063-72, 2006; e. g. Melocont® from Agrifutur, Agrianello, Italy), Bradyrhizobium sp. (e. g. Vault® from BASF Corp., USA), B . sp. ( Arachis ) CB1015 presumably originally collected in India (IITA 1006, USDA 3446; from Australian Inoculants Research Group; http://www.qaseeds.com.au/inoculant_applic.php). B . sp. ( Arachis ) strains deposited at SEMIA and known from FEMS Microbiol.
  • B. japonicum 532c isolated from Wisconsin field (Nitragin 61A152; Can. J. Plant. Sci. 70, 661-666, 1990; e. g. in Rhizoflo®, Histick®, Hicoat® Super from BASF Agricultural Specialties Ltd., Canada), B. japonicum E-109 variant of strain USDA 138 (INTA E109, SEMIA 5085; Eur. J. Soil Biol. 45, 28-35, 2009; Biol. Fertil. Soils 47, 81-89, 2011), B. japonicum G49 (MSDJ G49; C. R. Acad. Agric. Fr. 73, 163-171, 1987); B.
  • SEMIA 566 isolated from North American inoculant in 1966 and used in Brazilian commercial inoculants from 1966 to 1978, SEMIA 586 originally isolated in Maryland, USA, in 1961 but received from Australia in 1966 and used in Brazilian inoculants in 1977 (CB 1809, USDA 136, Nitragin 61A136, RCR 3407), SEMIA 5079 a natural variant of SEMIA 566 used in commercial inoculants since 1992 (CPAC 15; e. g. GELFIX 5 or ADHERE 60 from BASF Agricultural Specialties Ltd., Brazil), B.
  • japonicum SEMIA 5080 a natural variant of SEMIA 586 used in commercial inoculants since 1992 (CPAC 7; e. g. GELFIX 5 or ADHERE 60 from BASF Agricultural Specialties Ltd., Brazil); B. japonicum TA-11 (TA11 NOD+) (NRRL B-18466; U.S. Pat. No. 5,021,076; Appl. Environ. Microbiol. 56, 2399-2403, 1990; e. g. VAULT® NP, from BASF Corp., USA), B. japonicum strains deposited at USDA known from U.S. Pat. No. 7,262,151 and Appl. Environ. Microbiol.
  • USDA 3 isolated from Glycine max in Virginia (USA) in 1914
  • Serogroup 31 isolated from Glycine max in Wisconsin (USA) in 1941
  • USDA 76 isolated from plant passage of strain USDA 74 (Serogroup 76) which has been isolated from G. max in California (USA) in 1956
  • oleophila strain O NRRL Y-2317; Biological Control 51, 403-408, 2009
  • Candida saitoana e. g. Biocure® [in mixture with lysozyme] and BioCoat® from Micro Flo Company, USA (BASF SE) and Arysta
  • chitosan e. g. Armour-Zen® from BotriZen Ltd., NZ
  • Clonostachys rosea f. catenulate also named Gliocladium catenulatum ) J1446 isolated from Finnish field soil (NJF seminar No 389: Pest, disease and weed management in strawberry; Finland 8-9. Nov. 2006 in NJF Report 2(10), 15-15, 2006; DSM 9212; e. g.
  • CrleGV Cryptophlebia leucotreta granulovirus
  • CpGV Cydia pomonella granulovirus
  • DSM GV-0006 DSM GV-0006; e. g. Madex® Max from Andermatt Biocontrol, Switzerland
  • CpGV V22 DSM GV-0014; e. g.
  • fumosorosea FE 9901 ARSEF 4490; Biocontrol Science Technol. 22(7), 747-761, 2012; e. g. blastospores in NoFlyTM WP from Natural Industries, Inc., Houston, Tex., USA or from Novozymes, U.S.A.), cis-jasmone (U.S. Pat. No. 6,890,525; U.S. Pat. No. 8,221,736; Plant Bioscience Limited, Norwich, U.K.), laminarin (e. g. in Vacciplant® from Laboratoires Goemar, St. Malo, France or Stahler SA, Switzerland), Lecanicillium longisporum KV42 and KV71 (e. g.
  • Vertalec® from Koppert BV, Netherlands L. muscarium Ve6 (also called KV01; IMI 19-79, CABI 268317, CBS 102071, ARSEF 5128; e. g. Mycotal® from Koppert BV, Netherlands), Lysobacter antibioticus 13-1 (Biological Control 45, 288-296, 2008), L. antibioticus HS124 (Curr. Microbiol. 59(6), 608-615, 2009), L. enzymogenes 3.1T8 (Microbiol. Res. 158, 107-115, 2003; Biological Control 31(2), 145-154, 2004); Mesorhizobium spp. strains known from Soil Biol. Biochem.
  • M . sp. WSM1271 collected in Sardinia, Italy, from plant host Biserrula pelecinus, M . sp. WSM 1497 collected in Mykonos, Greece, from Biserrula pelecinus, Mesorhizobium ciceri CC1192 collected in Israel from Cicer arietinum nodules (UPM 848, CECT 5549; Can. J. Microbiol. 48, 279-284, 2002; from Horticultural Research Station, Gosford, Australia), M.
  • huakuii HN3015 isolated from Astralagus sinicus in a rice-growing field of Southern China (World J. Microbiol. Biotechn. 23(6), 845-851, 2007, ISSN 0959-3993), M. loti CC829 isolated from L. ulginosus nodules in USA (NZP 2012; commerical inoculant for Lotus pedunculatus and L. ulginosus in Australia), and M. loti SU343 isolated from host nodules in USA (commercial inoculant for Lotus corniculatus in Australia); Metarhizium anisopliae FI-1045 (AGAL V10/0104285; WO 2012/018266; e. g.
  • M. anisopliae var. anisopliae F52 also called 275 or V275 DSM 3884, ATCC 90448; e. g. Met52® Novozymes Biologicals BioAg Group, Canada
  • M. anisopliae ICIPE 69 isolated from a soil sample obtained from the Democratic Republic of Congo (DRC) and using the Galleria bait method in 1990 e. g. Metathripol from ICIPE, Nairobe, Kenya
  • Green Muscle® from BASF Agricultural Specialities (Pty) Ltd., South Africa), M . a. var. acridum F1-985 isolated from a spur-throated locust, Austracris guttulosa (Walker), near Rockhampton, Queensland, Australia, in 1979 (ARSEF 324; Memoirs of the Entomological Society of Canada 171, 287-300, 1997; e. g. Green Guard® SC from BASF Agricultural Specialties Pty Ltd, Australia), Metschnikowia fructicola 277 isolated from the surface of grape berries (cv. Superior) grown in the central part of Israel (U.S. Pat. No. 6,994,849; NRRL Y-30752; e. g.
  • MuscudorTM or QRD300 from AgraQuest, USA Muscodor albus SA-13 (NRRL B-50774; US 2014/0086879 A1; e. g. MBI-601-EP from Marrone BioInnovations, Inc., USA), Neem oil (e. g. Trilogy®, Triact® 70 EC from Certis LLC, USA), Nomuraea rileyi strains SM6101, GU87401, SR86151, CG128 and VA9101 (Braz. Arch. Biol. Technol.
  • Ph3 isolated from turfgrass soil samples collected at the DeBary Golf Course in central Florida (ATCC SD-5832; WO 2012/064527; for control of Hoplolaimus galeatus nematode from Pasteuria Bioscience, Inc. now Syngenta Crop Protection, LLC, USA), Pasteuria sp. Pr3 isolated from soil samples collected in the south-eastern United States (ATCC SD-5834; for control of Rotylenchulus reniformis nematode potentially of species P. ramosa ; Naviva® ST from Syngenta Crop Protection, LLC, USA), P. nishizawae (WO 2010/80619), P.
  • nishizawae Pn1 (Federal Register 76(22), 5808, Feb. 2, 2011; ATCC SD-5833; e.g. ClarivaTM PN from Syngenta Crop Protection, LLC, USA), P. penetrans (U.S. Pat. No. 5,248,500; Del Monte Corp.), P. ramosa (WO 2010/080619), P. thornea (WO 2010/080619), P. usgae BL1 (ATCC SD-5835; J. Nematol. 42(2): 87-90, 2010; ibid. 43(2), 101-109, 2011; e. g.
  • Penicillium bilaiae also called P. bilaii
  • ATCC 18309 ATCC 74319
  • ATCC 20851 ATCC 20851
  • ATCC 22348 ATCC 743178
  • Reynoutria sachalinensis extract (EP 0307510 B1; e. g. Regalia® SC from Marrone BioInnovations, Davis, Calif., USA or Milsana® from BioFa AG, Germany), Rhizobium leguminosarum bv. phaseoli (e. g. RHIZO-STICK from BASF Corp., USA), R. leguminosarum bv. phaseoli RG-B10 (USDA 9041; from Int. J. Syst. Bacteriol. 46(1), 240-244, 1996; Int. J. Syst. Evol. Microbiol. 50, 159-170, 2000; e. g.
  • trifolii TA1 Appl. Environ. Microbiol. 49(1), 127-131, 1985; e. g. Nodulaid® peat for white clover from BASF Agricultural Specialties Pty Ltd, Australia
  • R. I . bv. viciae RG-P2 also called P2 isolated from pea root nodules in Sakatchewan, Canada (e. g RhizUP peat for peas and lentils in Canada from BASF Agricultural Specialties Ltd., Canada), R. I . bv. viciae SU303 (e. g. Nodulaid® Group E from BASF Agricultural Specialties Pty Ltd, Australia), R. I .
  • WSM1455 e. g. Nodulaid® Group F from BASF Agricultural Specialties Pty Ltd, Australia
  • R. tropici CC511 Agronomy, N.Z. 36, 4-35, 2006; e. g. Nodulaid® peat for common bean from BASF Agricultural Specialties Pty Ltd, Australia
  • SMCD2220-01 I DAC 301008-01; WO 2011/022809
  • SpliNPV Spodoptera littoralis nucleopolyhedrovirus
  • Steinernema carpocapsae e. g. Millenium® from BASF Agricultural Specialities Limited, UK
  • feltiae (Nemashield® from BioWorks, Inc., USA; Nemasys® from BASF Agricultural Specialities Limited, UK), S. kraussei L137 (Nemasys® L from BASF Agricultural Specialities Limited, UK), Streptomyces galbus AQ6047 (NRRL 30232; WO 2012/135763; AgraQuest now Bayer CropScience LP, USA); S. galbus M1064 (NRRL 50334; WO 2012/135763; AgraQuest now Bayer CropScience LP, USA); S. griseoviridis K61 (Crop Protection 25, 468-475, 2006; e. g. Mycostop® from Verdera Oy, Espoo, Finland), S.
  • lydicus WYEC 108 (U.S. Pat. No. 5,403,584; e. g. Actinovate® from Natural Industries, Inc., USA), S. violaceusniger YCED-9 (U.S. Pat. No. 5,968,503; e. g. DT-9® from Natural Industries, Inc., USA), Talaromyces flavus V117b isolated from soil (e. g. Protus® WG from Prophyta, Germany), and Ulocladium oudemansii HRU3 (Agronomy 3, 632-647, 2013; e. g. Botry-Zen® from Botry-Zen Ltd, NZ).
  • BR Embrapa Agrobiology Diazothrophic Microbial Culture Collection, P.O.Box 74.505, Seropedica, Rio de Janeiro, 23.851-970, Brazil
  • CABI or IMI CABI Europe—International Mycological Institute, Bakeham Lane, Egham, Surrey, TW20 9TYNRRL, UK
  • CB The CB Rhizobium Collection, School of Environment and Agriculture, University of Western Sydney, Hawkesbury, Locked Bag 1797, South Penrith Distribution Centre, NSW 1797, Australia
  • CBS Centraalbureau voor Schimmelcultures, Fungal Biodiversity Centre, Uppsalaan 8, PO Box 85167, 3508 AD Utrecht, Netherlands
  • CC Division of Plant Industry, CSIRO, Canberra, Australia
  • CNCM Collection Nationale de Cultures de Microorganismes, Institute Pasteur, 25 rue du Dondel Roux, F-75724 PARIS Cedex 15
  • CPAC Embrapa-Cerrados, CX.
  • Nitragin Nitragin strain collection, The Nitragin Company, Milwaukee, Wis., USA, NRRL or ARSEF (collection of entomopathogenic fungi): ARS Culture Collection of the National Center for Agricultural Utilization Research, Agricultural Research Service, U.S. Department of Agriculture, 1815 North University Street, Peoria, Ill.
  • NZP Department of Scientific and Industrial Research Culture Collection, Applied Biochemistry Division, Palmerston North, New Zealand
  • PPRI ARC-Plant Protection Research Institute, Private Bag X134, Queenswood Pretoria, Gauteng, 0121, South Africa
  • SEMIA FEPAGRO-Fundaç ⁇ o Estadual de Pesquisa Agropecuária, Rua Gonçalves Dias, 570, Bairro Menino Deus, Porto Alegre/RS, Brazil
  • SRDI SARDI, Sydney, South Australia
  • USDA U.S. Department of Agriculture, Agricultural Research Service, Soybean and Alfalfa Research Laboratory, BARC-West, 10300 Baltimore Boulevard, Building 011, Beltsville, Md.
  • Jasmonic acid its salts (jasmonates) or derivatives include without limitation potassium, sodium, lithium, ammonium, dimethylammonium, isopropylammonium, diolammonium and diethtriethanolammonium jasmonate; and also jasmonic acid methyl ester, jasmonic acid amide, jasmonic acid methylamide, jasmonic acid-L-amino acid (amide-linked) conjugates (e. g.
  • L-isoleucine, L-valine, L-leucine, or L-phenylalanine 12-oxo-phytodienoic acid, coronatine, coronalon, coronafacoyl-L-serine, coronafacoyl-L-threonine, methyl esters of 1-oxo-indanoyl-isoleucine, methyl esters of 1-oxo-indanoyl-leucine, cis-jasmone, linoleic acid or derivatives thereof, and combinations of any of the above.
  • Humates are humic and fulvic acids extracted from a form of lignite coal and clay, known as leonardite.
  • Humic acids are organic acids that occur in humus and other organically derived materials such as peat and certain soft coal. They have been shown to increase fertilizer efficiency in phosphate and micro-nutrient uptake by plants as well as aiding in the development of plant root systems.
  • the at least one pesticide II is selected from the groups L1) to L6):
  • the present invention furthermore relates to agrochemical compositions comprising a mixture of at least one microorganism I (component 1) and at least one biopesticide selected from the group L) (component 2), in particular at least one further fungicidal biopesticide selected from the groups L1) and L2), as described above, and if desired at least one suitable auxiliary.
  • mixtures comprising as pesticide II (component 2) a biopesticide from group L1), preferably selected from Bacillus amyloliquefaciens herein even more preferably from strains IN937a, IT-45; B. amyloliquefaciens ssp. plantarum (formerly called B. subtilis or B. subtilis spp. amyloliquefaciens ) herein even more preferably from strains MBI600, D747, FZB254, FZB42, GB03, and QST-713; B. pumilus herein even more preferably from strains GHA 180, INR-7, KFP9F and QST 2808; B.
  • a biopesticide from group L1 preferably selected from Bacillus amyloliquefaciens herein even more preferably from strains IN937a, IT-45; B. amyloliquefaciens ssp. plantarum (formerly called B. subtilis or B. subtilis s
  • strain ABU 288 simplex herein more preferably strain ABU 288; B. solisalsi; B. subtilis herein even more preferably selected from strains CX-9060, FB17 and GB07; Muscodor albus herein more preferably strains QST 20799 and SA-13; Paenibacillus alvei herein more preferably strain NAS6G6, Paenibacillus polymyxa herein more preferably strain PKB1, Penicillium bilaiae herein more preferably strains ATCC 22348, ATCC 20581 and ATCC 18309; Pseudomonas fluorescens herein more preferably strain A506; Sphaerodes mycoparasitica herein more preferably strain SMCD2220.
  • mixtures comprising as pesticide II (component 2) a biopesticide from group L1), even more preferably selected from even more preferably from B. amyloliquefaciens ssp. plantarum M B1600, B. amyloliquefaciens ssp. plantarum QST-713, B. pumilus INR-7, B. pumilus QST 2808, B. simplex ABU 288, B. subtilis FB17, and Paenibacillus alvei NAS6G6.
  • the at least one pesticide II is Bacillus amyloliquefaciens ssp. plantarum MBI600. These mixtures are particularly suitable in soybean.
  • the at least one pesticide II is B. pumilus INR-7. These mixtures are particularly suitable in soybean and corn.
  • the at least one pesticide II is Bacillus simplex , preferably B. simplex ABU 288. These mixtures are particularly suitable in soybean and corn.
  • the at least one pesticide II is Bacillus subtilis , preferably B. subtilis strain FB17.
  • the at least one pesticide II is selected from Bacillus amyloliquefaciens spp. plantarum FZB24, B. amyloliquefaciens ssp. plantarum FZB42, B. amyloliquefaciens ssp. plantarum D747, B. amyloliquefaciens ssp. plantarum MBI600, B. amyloliquefaciens spp. plantarum GB03, B. amyloliquefaciens spp. plantarum QST-713, B. pumilus GB34, B. pumilus INR-7, B. pumilus KFP9F, B.
  • pumilus QST 2808 B. pumilus GHA 180, B. simplex ABU 288, B. subtilis CX-9060, B. subtilis FB17 and B . subtilis GB07. These mixtures are particularly suitable in soybean and corn, in particular for seed treatment.
  • the at least one pesticide II is Coniothyrium minitans CON/M/91-08. These mixtures are particularly suitable for seed and/or soil treatment.
  • the at least one pesticide II is selected from Pseudomonas spp., preferably selected from P. chloraphis herein more preferably strain MA 342 and Pseudomonas sp. DSM 13134; P. fluorescens herein more preferably selected from strains A506, WCS 374 and Pf-5; and P. putida herein more preferably strain ATCC 202153.
  • the present invention also relates to mixtures wherein the at least one pesticide II is selected from the fungal species Muscodor albus preferably from the strains SA-13 and QST 20799, which are particularly suiable for soil and seed treatment against soil-borne pathogens and/or nematodes.
  • mixtures comprising as pesticide II (component 2) a biopesticide from group L2), preferably selected from chitosan (hydrolysate), methyl-jasmonate, cis-jasmone, laminarin, Reynoutria sachalinensis extract and tea tree oil; even more preferable from methyl jasmonate and cis-jasmone.
  • a biopesticide from group L2 preferably selected from chitosan (hydrolysate), methyl-jasmonate, cis-jasmone, laminarin, Reynoutria sachalinensis extract and tea tree oil; even more preferable from methyl jasmonate and cis-jasmone.
  • mixtures comprising as pesticide II (component 2) a biopesticide from group L3), preferably selected from Agrobacterium radiobacter herein preferably strain K1026, Bacillus firmus herein referably strain I-1582, Bacillus thuringiensis ssp. kurstaki herein preferably strain SB4, Beauveria bassiana herein preferably selected from strains GHA, H123, DSM 12256 and PPRI 5339; Burkholderia sp. and herein preferably strain A396, Metarhizium anisopliae var. acridum herein preferably strain IMI 330189, M.
  • pesticide II component 2
  • component 2 a biopesticide from group L3
  • Agrobacterium radiobacter preferably strain K1026, Bacillus firmus herein referably strain I-1582, Bacillus thuringiensis ssp. kurstaki herein preferably strain SB4, Beauveria bassiana herein preferably selected from strains GHA, H123, DSM 12
  • anisopliae herein preferably selected from strains FI-985, FI-1045, F52 and ICIPE 69; Paecilomyces lilacinus herein preferably selected from strains 251, DSM 15169 and BCP2, Paenibacillus popilliae herein preferably selected from strains Dutky-1940, KLN 3 and Dutky 1; Pasteuria nishazawa and herein preferably strain Pn1.
  • mixtures comprising as pesticide II (component 2) a biopesticide from group L3), even more preferably from Bacillus thuringiensis ssp. kurstaki SB4, B. bassiana DSM 12256, B. bassiana PPRI 5339, Paecilomyces lilacinus DSM 15169, P. lilacinus BCP2, P. lilacinus 251, Paenibacillus popilliae Dutky-1940, P. popilliae KLN 3 and P. popilliae Dutky 1.
  • the at least one pesticide II is Beauveria bassiana PPRI 5339.
  • the at least one pesticide II is Bacillus firmus , preferably spores of strain CNCM I-1582, preferably useful for seed treatment of soybean and corn against nematodes and insects.
  • the at least one pesticide II is Bacillus cereus , preferably spores of CNCM I-1562, preferably useful for seed treatment of soybean and corn against nematodes and insects.
  • the at least one pesticide II is a mixture of spores of B. firmus and B. cereus , preferably mixtures spores of above mentioned strains CNCM I-1582 and CNCM I-1562, preferably useful for seed treatment of soybean and corn against nematodes and insects.
  • the at least one pesticide II is selected from Bacillus t . ssp. kurstaki preferably from strains EG 2348, SB4 and ABTS-351 (HD-1), in particular B. t . ssp. kurstaki SB4. These strains are used for control of lepidopteran larvae, but without noctuidae.
  • the at least one pesticide II is selected from Bacillus firmus CNCM I-1582, Paecilomyces lilcinus 251, Pasteuria nishizawa Pn1 and Burkholderia sp. A396 having nematicidal, acaricidal and/or insecticidal activity. These mixtures are particularly suitable in soybean and corn, in particular for seed treatment.
  • mixtures comprising as pesticide II (component 2) a biopesticide from group L4), preferably selected from methyl jasmonate, Acacia negra extract, extract of grapefruit seeds and pulp, Catnip oil, Neem oil, Quillay extract and Tagetes oil, in particular methyl jasmonate or water-based Quillay extract.
  • a biopesticide from group L4 preferably selected from methyl jasmonate, Acacia negra extract, extract of grapefruit seeds and pulp, Catnip oil, Neem oil, Quillay extract and Tagetes oil, in particular methyl jasmonate or water-based Quillay extract.
  • mixtures comprising as pesticide II (component 2) a biopesticide from group L5), preferably selected from Azospialum amazonense, A. brasllense, A. lipoferum, A. irakense, A. halopraeferens, Bradyrhizobium sp. ( Vigna ), B. elkanil; B. japonicum Paembacillus alvei, Penicilium bilaiae, Rhizobium legu minosarum bv. phaseoli; R. I . bv. trifolii, R. I . bv. viciae , and Sinorhizobium meliloti.
  • a biopesticide from group L5 preferably selected from Azospialum amazonense, A. brasllense, A. lipoferum, A. irakense, A. halopraeferens, Bradyrhizobium sp. ( Vigna ), B. elkanil;
  • mixtures comprising as pesticide II (component 2) a biopesticide from group L5) selectedifrom Azospialum amazonense SpY2 T , A. brasllense XOH, A. brasilense Sp245, A. brasilense Cd, A. brasllense Ab-V5, A. brasilense Ab-V6, A. lipoferum Sp31, Bradyrhizobium sp. ( Vigna ) PNL1, B. elkanii SEMIA 587, B. elkanii SEMIA 5019, B. japonicum SEMIA 5079, B. japonicum SEMIA 5080, B. japonicum TA-11, B.
  • pesticide II component 2
  • component 2 a biopesticide from group L5 selectedifrom Azospialum amazonense SpY2 T , A. brasllense XOH, A. brasilense Sp245, A. brasilense Cd, A. brasllense Ab-V5, A. brasil
  • japonicum 532c Paembacillus alvei NAS6G6, Peniciillium bilaiae strains ATCC 18309, ATCC 20851 and ATCC 22348; Rhizobium leguminosarum bv. phaseoli P1NP3Cst, R. I . bv. phaseoli RG-B10, R. I . bv. trifolii RP113-7, R. I . bv. viciae SU303, R. I . bv. viciae WSM1455, R. tropici SEMIA 4077, R.
  • tropici PRF 81 and Sinorhizobium meliloti even more preferably selected from Azospirillum brasilense Sp245, Bradyrhizobium sp. ( Vigna ) PNL1, B. elkanii SEMIA 587, B. elkanii SEMIA 5019, B. japonicum SEMIA 5079, B. japonicum SEMIA 5080, B. japonicum TA-11 and B. japonicum 532c.
  • component 2) in the mixtures comprises at least one biopesticide II strain selected from Azospirillum brasilense Sp 245, A. brasilense Ab-V5, A. brasilense Ab-V6, Bradyrhizobium elkanii SEMIA 5019, B. elkanii SEMIA 587, B. japonicum 532c, B. japonicum E-109, B. japonicum SEMIA 5079 and B. japonicum SEMIA 5080.
  • biopesticide II strain selected from Azospirillum brasilense Sp 245, A. brasilense Ab-V5, A. brasilense Ab-V6, Bradyrhizobium elkanii SEMIA 5019, B. elkanii SEMIA 587, B. japonicum 532c, B. japonicum E-109, B. japonicum SEMIA 5079 and B. japonicum SEMIA 5080.
  • the present invention also relates to mixtures, wherein the at least one pesticide II is selected from B. japonicum and B. elkanii and further comprises a pesticide III, wherein pesticide III is selected from jasmonic acid, its salts and derivatives thereof, preferably methyl-jasmonate or cis-jasmone.
  • the present invention also relates to mixtures wherein the at least one pesticide II is selected from Bacillus amyloliquefaciens ssp. plantarum M B1600, B. amyloliquefaciens ssp. plantarum FZB24, B. amyloliquefaciens ssp. plantarum FZB42, B. amyloliquefaciens ssp. plantarum D747, B. amyloliquefaciens ssp. plantarum QST-713, B. firmus CNCM I-1582, B. pumilus GHA 180, B. pumilus INR-7, B. pumilus QST 2808, B. simplex ABU 288, B.
  • the at least one pesticide II is selected from Bacillus amyloliquefaciens ssp. plantarum M B1600, B. amyloliquefaciens ssp. plantarum FZB24, B
  • subtilis FB17 Burkholderia sp. A396, Coniothyrium minitans CON/M/91-08, Paecllomyces lilacinus 251, Paenibacillus alvei NAS6G6, Pasteuria nishizawae Pn1, P. bilaiae ATCC 18309, P. bilaiae ATCC 20851, P. bilaiae ATCC 22348; cis-jasmone, methyl jasmonate and harpin protein
  • the pesticides II of chemical nature are described by common names, their preparation and their activity against pests is known (cf.: http://www.alanwood.net/pesticides/); these pesticides are often commercially available.
  • mixtures comprising as component 2) at least one pesticide II from the groups A) to H), N) and O) selected from:
  • mixtures comprising as component 2) at least one pesticide II from pyraclostrobin, azoxystrobin, trifloxystobin; fluxapyroxad, penflufen, sedaxane, fluopyram; ipconazole; oxathiapiprolin, valifenalate; fipronil; imidacloprid; chlorantraniliprole; and cyantraniliprole.
  • mixtures comprising as component 1) B. amyloliquefaciens AP-188 and as component 2) at least one pesticide II from pyraclostrobin, azoxystrobin, trifloxystobin; fluxapyroxad, penflufen, sedaxane, fluopyram; ipconazole; oxathiapiprolin, valifenalate; fipronil; imidacloprid; chlorantraniliprole; and cyantraniliprole.
  • inventive mixtures comprising at least one microorganism I and/or as pesticide II a microbial pesticide from groups L1), L3) and L5) may be formulated as an inoculant for a plant.
  • inoculant means a preparation that includes an isolated culture of a microbial pesticide and optionally a carrier, which may include a biologically acceptable medium.
  • microbial pesticides may be supplied in any physiological state such as active or dormant.
  • Dormant microbial pesticides may be supplied for example frozen, dried, or lyophilized or partly desiccated (procedures to produce partly desiccated organisms are given in WO 2008/002371) or in form of spores.
  • Microbial pesticides II selected from groups L1), L3) and L5) and the at least one microorganism I used as organisms in an active state can be delivered in a growth medium without any additional additives or materials or in combination with suitable nutrient mixtures.
  • the at least one microorganism I is preferably delivered and formulated in a dormant stage, more preferably in form of spores.
  • the mixtures and compositions according to the invention are particularly important in the control of a multitude of phytopathogenic fungi on various cultivated plants; and on the plant propagation material, such as seeds, and the crop material of these plants.
  • inventive mixtures and compositions are used for controlling a multitude of fungi on the following cultivated plants: field crops, such as potatoes sugar beets, tobacco, wheat, rye, barley, oats, rice, corn, cotton, soybeans, rape, legumes, sunflowers, coffee or sugar cane; fruits; vines; ornamentals; or vegetables, such as cucumbers, tomatoes, beans or squashes.
  • field crops such as potatoes sugar beets, tobacco, wheat, rye, barley, oats, rice, corn, cotton, soybeans, rape, legumes, sunflowers, coffee or sugar cane
  • fruits vines; ornamentals
  • vegetables such as cucumbers, tomatoes, beans or squashes.
  • plant propagation material is to be understood to denote all the generative parts of the plant such as seeds and vegetative plant material such as cuttings and tubers (e. g. potatoes), which can be used for the multiplication of the plant. This includes seeds, roots, fruits, tubers, bulbs, rhizomes, shoots, sprouts and other parts of plants, including seedlings and young plants, which are to be transplanted after germination or after emergence from soil.
  • vegetative plant material such as cuttings and tubers (e. g. potatoes), which can be used for the multiplication of the plant. This includes seeds, roots, fruits, tubers, bulbs, rhizomes, shoots, sprouts and other parts of plants, including seedlings and young plants, which are to be transplanted after germination or after emergence from soil.
  • treatment of plant propagation materials with the inventive mixtures and compositions thereof, respectively is used for controlling a multitude of fungi on cereals, such as wheat, rye, barley and oats; rice, corn, cotton and soybeans.
  • cultiva plants is to be understood as including plants which have been modified by breeding, mutagenesis or genetic engineering including but not limiting to agricultural biotech products on the market or in development (cf. http://cera-gmc.org/, see GM crop database therein).
  • Genetically modified plants are plants, which genetic material has been so modified by the use of recombinant DNA techniques that under natural circumstances cannot readily be obtained by cross breeding, mutations or natural recombination.
  • one or more genes have been integrated into the genetic material of a genetically modified plant in order to improve certain properties of the plant.
  • inventive mixtures and compositions are particularly suitable for controlling the following plant diseases:
  • Albugo spp. white rust on ornamentals, vegetables (e. g. A. candida ) and sunflowers (e. g. A. tragopogonis ); Altemana spp. (Alternaria leaf spot) on vegetables, rape ( A. brassicola or A. brassicae ), sugar beets ( A. tenuis ), fruits, rice, soybeans, potatoes (e. g. A. solani or A.retemata ), tomatoes (e. g. A. solani or A.retemata ) and wheat; Aphanomyces spp. on sugar beets and vegetables; Ascochyta spp. on cereals and vegetables, e. g. A.
  • tritici anthracnose
  • Bipolaris and Drechslera spp. (teleomorph: Cochliobolus spp.) on corn (e. g. D. maydis ), cereals (e. g. B. sorokiniana : spot blotch), rice (e. g. B. oryzae ) and turfs; Blumeria (formerly Erysiphe ) graminis (powdery mildew) on cereals (e. g. on wheat or barley); Botrytis cinerea (teleomorph: Botrifotinia fuckeliana : grey mold) on fruits and berries (e. g.
  • strawberries strawberries
  • vegetables e. g. lettuce, carrots, celery and cabbages
  • rape flowers, vines, forestry plants and wheat
  • Bremia lactucae downy mildew
  • Ceratocystis syn. Ophiostoma
  • spp. rot or wilt
  • broad-leaved trees and evergreens e. g. C. ulmi (Dutch elm disease) on elms
  • Cercospora spp. Cercospora leaf spots
  • corn rice
  • sugar beets e. g. C. beticola
  • sugar cane vegetables
  • coffee soybeans
  • Colletotrichum teleomorph: Glomerella
  • spp. anthracnose
  • cotton e. g. C. gossypii
  • corn e. g. C. graminicola
  • soft fruits e. g. C. coccodes black dot
  • beans e. g. C. lindemuthianum
  • soybeans e. g. C. truncatum or C. gloeosporioides
  • Corticium spp. e. g. C. C.
  • sasakii sheath blight
  • Corynespora cassiicola leaf spots
  • Cycloconium spp. e. g. C. oleaginum on olive trees
  • Cylindrocarpon spp. e. g. fruit tree canker or young vine decline, teleomorph: Nectria or Neonectria spp.
  • liriodendri teleomorph: Neonectria liriodendri : Black Foot Disease) and ornamentals; Dematophora (teleomorph: Rosellinia ) necatrix (root and stem rot) on soybeans; Diaporthe spp., e. g. D. phaseolorum (damping off) on soybeans; Drechslera (syn. Helminthosporium , teleomorph: Pyrenophora ) spp. on corn, cereals, such as barley (e. g. D. teres , net blotch) and wheat (e. g.
  • D tritici - repentis tan spot), rice and turf; Esca (dieback, apoplexy) on vines, caused by Formitipona (syn. Phellinus ) punctata, F. mediterranea, Phaeomoniella chlamydospora (earlier Phaeoacremonium chlamydosporum ), Phaeoacremonium aleophllum and/or Botryosphaeria obtusa; Elsinoe spp. on pome fruits ( E. pyri ), soft fruits ( E. veneta : anthracnose) and vines ( E.
  • ampelina anthracnose
  • Entyloma oryzae leaf smut
  • Epicoccum spp. black mold
  • Erysiphe spp. potowdery mildew
  • sugar beets E. betae
  • vegetables e. g. E. pisi
  • cucurbits e. g. E. cichoracearum
  • cabbages e. g. E. cruciferarum
  • Eutypa lata Eutypa canker or dieback, anamorph: Cytosporina lata , syn.
  • Phakopsora pachyrhizi and P. meibomiae on soybeans
  • Phialophora spp. e. g. on vines (e. g. P. tracheiphila and P. tetraspora ) and soybeans (e. g. P. gregata : stem rot); Phoma lingam (root and stem rot) on rape and cabbage and P. betae (root rot, leaf spot and damping-off) on sugar beets
  • Phomopsis spp. on sunflowers, vines e. g. P. viticola : can and leaf spot
  • soybeans e. g.
  • stem rot P. phaseoli , teleomorph: Diaporthe phaseolorum ); Physoderma maydis (brown spots) on corn; Phytophthora spp. (wilt, root, leaf, fruit and stem root) on various plants, such as paprika and cucurbits (e. g. P. capsici ), soybeans (e. g. P. megasperma , syn. P. sojae ), potatoes and tomatoes (e. g. P. infestans : late blight) and broad-leaved trees (e. g. P.
  • Pseudocercosporella herpotrichoides eyespot, teleomorph: Tapesia yallundae ) on cereals, e. g. wheat or barley
  • Pseudoperonospora downy mildew
  • Pseudopezicula tracheiphila red fire disease or ‘rotbrenner’, anamorph: Phialophora ) on vines
  • Puccinia spp. rusts
  • oryzae (teleomorph: Magnaporthe grisea , rice blast) on rice and P. grisea on turf and cereals; Pythium spp. (damping-off) on turf, rice, corn, wheat, cotton, rape, sunflowers, soybeans, sugar beets, vegetables and various other plants (e. g. P. ultimum or P. aphani - dermatum ); Ramularia spp., e. g. R. collo - cygni ( Ramularia leaf spots, Physiological leaf spots) on barley and R. beticola on sugar beets; Rhizoctonia spp.
  • R. solani root and stem rot
  • S. solani silk and stem rot
  • S. solani silk and stem rot
  • S. solani silk blight
  • rice or R. cerealis Rhizoctonia spring blight
  • Rhizopus stolonifer black mold, soft rot
  • strawberries carrots, cabbage, vines and tomatoes
  • Rhynchosporium secalis scald
  • Sarocladium oryzae and S. attenuatum sheath rot) on rice
  • Sclerotinia spp Sclerotinia spp.
  • seed rot or white mold on vegetables and field crops, such as rape, sunflowers (e. g. S. sclerotiorum ) and soybeans (e. g. S. rolfsii or S. sclerotiorum ); Septoria spp. on various plants, e. g. S. glycines (brown spot) on soybeans, S. tritici ( Septoria blotch) on wheat and S . (syn. Stagonospora ) nodorum ( Stagonospora blotch) on cereals; Uncinula (syn.
  • Erysiphe ) necator prowdery mildew, anamorph: Oidium tuckeri ) on vines
  • Setospaeria spp. leaf blight
  • corn e. g. S. turcicum , syn. Helminthosporium turcicum
  • turf e. g. S. turcicum , syn. Helminthosporium turcicum
  • Sphaerotheca fuliginea prowdery mildew
  • Spongospora subterranea powdery scab
  • the mixtures and compositions according to the invention are also suitable as bactericides. They are distinguished by an outstanding effectiveness against a broad spectrum of phytopathogenic bacteria, including soil-borne bacteria, which derive especially from the genera of Agrobacterium, Clavibacter, Corynebacterium, Erwinia, Leifsonia, Pectobacterium, Pseudomonas, Ralstonia, Xanthomonas (e.g. Xanthomonas oryzae causing bacterial blight on rice) and Xylella ; preferably Erwinia ; even more preferably Erwinia amylovora causing fire blight on apples, pears and other member of the family Rosaceae.
  • soil-borne bacteria which derive especially from the genera of Agrobacterium, Clavibacter, Corynebacterium, Erwinia, Leifsonia, Pectobacterium, Pseudomonas, Ralstonia, Xanthomonas (e.g. X
  • mixtures according to the present invention and compositions thereof, respectively, are also suitable for controlling harmful fungi in the protection of stored products or harvest.
  • the mixtures and compositions according to the invention are particularly important in the control of a multitude of phytopathogenic insects or other pests (e.g. lepidopterans, beetles, dipterans, thrips , heteropterans, hemiptera, homoptera, termites, orthopterans, arachnids, and nematodes) on various cultivated plants.
  • phytopathogenic insects or other pests e.g. lepidopterans, beetles, dipterans, thrips , heteropterans, hemiptera, homoptera, termites, orthopterans, arachnids, and nematodes
  • inventive mixtures and compositions are used for controlling a multitude of pests on field crops, such as potatoes sugar beets, tobacco, wheat, rye, barley, oats, rice, corn, cotton, soybeans, rape, legumes, sunflowers, coffee or sugar cane; fruits; vines; ornamentals; or vegetables, such as cucumbers, tomatoes, beans or squashes.
  • field crops such as potatoes sugar beets, tobacco, wheat, rye, barley, oats, rice, corn, cotton, soybeans, rape, legumes, sunflowers, coffee or sugar cane; fruits; vines; ornamentals; or vegetables, such as cucumbers, tomatoes, beans or squashes.
  • inventive mixtures and the compositions thereof, respectively, are particularly suitable for controlling the following harmful insects from the order of the
  • lepidopterans for example Agrotis ypsilon, Agrotis segetum, Alabama argillacea, Anticarsia gemmatalis, Argyresthia conjugella, Autographa gamma, Bupalus piniarius, Cacoecia murinana, Capua reticulana, Cheimatobia brumata, Choristoneura fumiferana, Choristoneura occidentalis, Cirphis unipuncta, Cydia pomonella, Dendrolimus pini, Diaphania nitidalis, Diatraea grandiosella, Earias insulana, Elasmopalpus lignosellus, Eupoecilia ambiguella, Evetria bouliana, Feltia subterranea, Galleria mellonella, Grapholitha funebrana, Grapholitha molesta, Heliothis armigera, Heliothis viresc
  • beetles Coldoptera
  • Agrilus sinuatus for example Agrilus sinuatus, Agriotes lineatus, Agriotes obscurus, Amphimallus solstitialis, Anisandrus dispar, Anthonomus grandis, Anthonomus pomorum, Atomaria linearis, Blastophagus piniperda, Blitophaga undata, Bruchus rufimanus, Bruchus pisorum, Bruchus lentis, Byctiscus betulae, Cassida nebulosa, Cerotoma trifurcata, Ceuthorrhynchus assimilis, Ceuthorrhynchus napi, Chaetocnema tibialis, Conoderus vespertinus, Crioceris asparagi, Diabrotica longicornis, Diabrotica speciosa, Diabrotica 12- punctata, Diabrotica virgifera, Diloboderus abderus, Epilachn
  • dipterans dipterans
  • Aedes aegypti Aedes vexans, Anastrepha ludens, Anopheles maculipennis, Ceratitis capitata, Chrysomya bezziana, Chrysomya hominivorax, Chrysomya macellaria, Contarinia sorghicola, Cordylobia anthropophaga, Culex pipiens, Dacus cucurbitae, Dacus oleae, Dasineura brassicae, Fannia canicularis, Gasterophilus intestinalis, Glossina morsitans, Haematobia irritans, HaplodiplosIS equestris, Hylemyia platura, Hypoderma lineata, Liriomyza sativae, Liriomyza trifolil, Lucilia caprin, Lucilia cuprina, Lucilia sericata, Lycoria pectoralis, Maye
  • Thrips e.g. Frankliniella fusca, Frankliniella occidentalis, Frankliniella tritici, Scirtothrips citri, Thrips oryzae, Thrips palmi and Thrips tabaci,
  • hymenopterans e.g. Acromyrmex ambuguus, Acromyrmex crassispinus, Acromyrmex heiery, Acromyrmex landolti, Acromyrmex subterraneus, Athalia rosae, Atta capiguara, Atta cephalotes, Atta laevigata, Atta robusta, Atta sexdens, Atta texana, Hoplocampa minuta, Hoplocampa testudinea, Monomorium pharaonic, Solenopsis geminata and Solenopsis invicta,
  • Hymenoptera e.g. Acromyrmex ambuguus, Acromyrmex crassispinus, Acromyrmex heiery, Acromyrmex landolti, Acromyrmex subterraneus, Athalia rosae, Atta capiguara, Atta
  • Heteroptera e.g. Acrosternum Mare, Blissus leucopterus, Cyrtopeltis notatus, Dichelops furcatus, Dysdercus cingulatus, Dysdercus intermedius, Euchistos heros, Eurygaster integriceps, Euschistus impictiventris, Leptoglossus phyllopus, Lygus lineolaris, Lygus pratensis, Nezara viridula, Piesma quadrata, Piezodorus gulldim, Solubea insularis and Thyanta perditor,
  • Heteroptera e.g. Acrosternum Mare, Blissus leucopterus, Cyrtopeltis notatus, Dichelops furcatus, Dysdercus cingulatus, Dysdercus intermedius, Euchistos heros, Eurygaster integriceps, Euschistus impictiventri
  • Isoptera e.g. Calotermes flavicollis, Cornitermes cumulans, Heterotermes tenuis, Leucotermes flavipes, Neocapritemes opacus, Procornitermes triacifer; Reticulitermes lucifugus, Syntermes molestus , and Termes natalensis,
  • orthopterans e.g. Acheta domestica, Blatta orientalis, Blattella germanica, Forticula auricularia, Gryllotalpa gryllotalpa, Locusta migratoria, Melanoplus bivittatus, Melanoplus femur - rubrum, Melanoplus mexicanus, Melanoplus sanguinipes, Melanoplus spretus, Nomadacris septemfasciata, Periplaneta americana, Schistocerca americana, Schistocerca peregrina, Stauronotus maroccanus and Tachycines asynamorus,
  • Orthoptera e.g. Acheta domestica, Blatta orientalis, Blattella germanica, Forticula auricularia, Gryllotalpa gryllotalpa, Locusta migratoria, Melanoplus bivittatus, Melanoplus femur - rubrum, Melanoplus mex
  • Arachnoidea such as arachnids, e.g. of the families Argasidae, Ixodidae and Sarcoptidae, such as Amblyomma americanum, Amblyomma variegatum, Argas persicus, Boophilus annulatus, Boophilus decoloratus, Boophilus microplus, Dermacentor silvarum, Hyalomma truncatum, Ixodes ricinus, Ixodes rubicundus, Ornithodorus moubata, Otobius megnini, Dermanyssus gallinae, Psoroptes ovis, Rhipicephalus appendiculatus, Rhipicephalus evertsi, Sarcoptes scabiei , and Eriophyidae spp.
  • arachnids e.g. of the families Argasidae, Ixodidae and Sarcoptidae, such as
  • Tetranychidae spp. such as Tetranychus cinnabarinus, Tetranychus kanzawai, Tetranychus pacificus, Tetranychus telarius and Tetranychus urticae, Panonychus ulmi, Panonychus citri , and Oligonychus pratensis.
  • inventive mixtures are suitable for combating pests of the orders Coleoptera, Lepidoptera, Thysanoptera, Homoptera, Isoptera, and Orthoptera.
  • nematodes such as root-knot nematodes, Meloidogyne arenaria, Meloidogyne chitwoodi, Meloidogyne exigua, Meloidogyne hapla, Meloidogyne incognita, Meloidogyne javanica and other Meloidogyne species; cyst nematodes, Globodera rostochiensis, Globodera pallida, Globodera tabacum and other Globodera species, Heterodera avenae, Heterodera glycines, Heterodera schachtii, Heterodera trifolii and other Heterodera species; seed gall nematodes, Anguina funesta, Anguina tritici and other Anguina species; stem and foliar nematodes, Aphelenchoides besseyi, Aphelenchoides fragariae, Ap
  • Plant propagation materials may be treated with the mixtures and compositions of the invention prophylactically either at or before planting or transplanting.
  • the present invention relates to a method for protection of plant propagation material from pests, wherein the plant propagation material is treated with an effective amount of an inventive mixture.
  • the present invention relates to a method for protection of plant propagation material from harmful fungi, wherein the plant propagation material is treated with an effective amount of an inventive mixture.
  • plant health is to be understood to denote a condition of the plant and/or its products which is determined by several indicators alone or in combination with each other such as yield (e. g. increased biomass and/or increased content of valuable ingredients), plant vigor (e. g. improved plant growth and/or greener leaves (“greening effect”)), quality (e. g. improved content or composition of certain ingredients) and tolerance to abiotic and/or biotic stress.
  • yield e. g. increased biomass and/or increased content of valuable ingredients
  • plant vigor e. g. improved plant growth and/or greener leaves (“greening effect”)
  • quality e. g. improved content or composition of certain ingredients
  • tolerance to abiotic and/or biotic stress e. g. improved content or composition of certain ingredients
  • the present invention relates to a method for improving the health of plants grown from said plant propagation material, wherein the plant propagation material is treated with an effective amount of an inventive mixture.
  • the present invention relates to a method for improving the health of plants, wherein the plant is treated with an effective amount of an inventive mixture.
  • Each plant health indicator such as yield, plant vigor, quality and tolerance of the plant to abiotic and/or biotic stress, is to be understood as a preferred embodiment of the present invention either each on its own.
  • the invention also relates to agrochemical compositions comprising an auxiliary and at least one microorganism I, or a cell-free extract thereof or at least one metabolite thereof having pesticidal activity, and/or a mutant of a microorganism I having pesticidal activity and producing at least one pesticidal metabolite as defined herein, or a pesticidal metabolite or extract of the mutant, and at least one pesticide II according to the invention.
  • An agrochemical composition comprises a fungicidally or insecticidally effective amount of at least one microorganism I and at least one pesticide II. Such an amount can vary in a broad range and is dependent on various factors, such as the fungal or pest species to be controlled, the treated cultivated plant or material, the climatic conditions.
  • the microorganisms as used according to the invention can be cultivated continuously or discontinuously in the batch process or in the fed batch or repeated fed batch process.
  • a review of known methods of cultivation will be found in the textbook by Chmiel (Bioreaktoren and periphere bamboo (Vieweg Verlag, Braunschweig/Wiesbaden, 1994)).
  • the culture medium that is to be used must satisfy the requirements of the particular strains in an appropriate manner.
  • individual components of the composition according to the invention such as parts of a kit or parts of a binary or ternary mixture may be mixed by the user himself in a spray tank or any other kind of vessel used for applications (e.g seed treater drums, seed pelleting machinery, knapsack sprayer) and further auxiliaries may be added, if appropriate.
  • a spray tank or any other kind of vessel used for applications e.g seed treater drums, seed pelleting machinery, knapsack sprayer
  • further auxiliaries may be added, if appropriate.
  • living microorganisms such as microorganisms I and pesticides II from groups L1), L3) and L5
  • the kit e.g. chemcial pesticidal agents
  • compatibility with the respective microbial pesticide has to be taken into account.
  • one embodiment of the invention is a kit for preparing a usable pesticidal composition, the kit compring a) a composition comprising component 1) as defined herein and at least one auxiliary; and b) a composition comprising component 2) as defined herein and at least one auxiliary.
  • the at least one pesticide II can be converted into customary types of agrochemical compositions, e. g. solutions, emulsions, suspensions, dusts, powders, pastes, granules, pressings, capsules, and mixtures thereof.
  • agrochemical compositions e. g. solutions, emulsions, suspensions, dusts, powders, pastes, granules, pressings, capsules, and mixtures thereof.
  • composition types are suspensions (e.g. SC, OD, FS), emulsifiable concentrates (e.g. EC), emulsions (e.g. EW, EO, ES, ME), capsules (e.g. CS, ZC), pastes, pastilles, wettable powders or dusts (e.g. WP, SP, WS, DP, DS), pressings (e.g.
  • compositions types are defined in the “Catalogue of pesticide formulation types and international coding system”, Technical Monograph No. 2, 6 th Ed. May 2008, CropLife International.
  • compositions are prepared in a known manner, such as described by Mollet and Grubemann, Formulation technology, Wiley VCH, Weinheim, 2001; or Knowles, New developments in crop protection product formulation, Agrow Reports DS243, T&F Informa, London, 2005.
  • compositions When living microorganisms, such as microorganisms I and pesticides II from groups L1), L3) and L5), form part of the compositions, such compositions can be prepared as compositions comprising besides the active ingredients at least one auxiliary (inert ingredient) by usual means (see e.g. H. D. Burges: Formulation of Micobial Biopestcides, Springer, 1998).
  • auxiliary inert ingredient
  • Suitable customary types of such compositions are suspensions, dusts, powders, pastes, granules, pressings, capsules, and mixtures thereof.
  • composition types are suspensions (e.g. SC, OD, FS), capsules (e.g. CS, ZC), pastes, pastilles, wettable powders or dusts (e.g.
  • auxiliaries examples are those mentioned earlier herein, wherein it must be taken care that choice and amounts of such auxiliaries should not influence the viability of the microbial pesticides in the composition.
  • bactericides and solvents compatibility with the respective microorganism of the respective microbial pesticide has to be taken into account.
  • compositions with microbial pesticides may further contain stabilizers or nutrients and UV protectants.
  • Suitable stabilzers or nutrients are e.g. alpha-tocopherol, trehalose, glutamate, potassium sorbate, various sugars like glucose, sucrose, lactose and maltodextrine (H. D. Burges: Formulation of Micobial Biopestcides, Springer, 1998).
  • Suitable UV protectants are e.g. inorganic compouns like titan dioxide, zinc oxide and iron oxide pigments or organic compounds like benzophenones, benzotriazoles and phenyltriazines.
  • the compositions may in addition to auxiliaries mentioned for compositions comprising compounds I herein optionally comprise 0.1-80% stabilizers or nutrients and 0.1-10% UV protectants.
  • the agrochemical compositions generally are characterized in that they contain an effective quantity of the active components as defined above. Generally, they contain between 0.01 and 95%, preferably between 0.1 and 90%, and in particular between 0.5 and 75%, by weight of active components, in particular active substances.
  • the compositions contain microbial pesticides such as TJ1000 and pesticides II from groups L1), L3) and L5) in an amount from 1 ⁇ 10 5 to 1 ⁇ 10 12 CFU, preferably from 1 ⁇ 10 7 CFU to 1 ⁇ 10 12 CFU, more preferably from 1 ⁇ 10 9 CFU to 1 ⁇ 10 12 CFU per gram total weight of the composition.
  • microbial pesticides such as TJ1000 and pesticides II from groups L1), L3) and L5 in an amount from 1 ⁇ 10 5 to 1 ⁇ 10 12 CFU, preferably from 1 ⁇ 10 7 CFU to 1 ⁇ 10 12 CFU, more preferably from 1 ⁇ 10 9 CFU to 1 ⁇ 10 12 CFU per gram total weight of the composition.
  • Solutions for seed treatment (LS), suspoemulsions (SE), flowable concentrates (FS), powders for dry treatment (DS), water-dispersible powders for slurry treatment (WS), water-soluble powders (SS), emulsions (ES), emulsifiable concentrates (EC) and gels (GF) are usually employed for the purposes of treatment of plant propagation materials, particularly seeds.
  • Preferred examples of seed treatment formulation types or soil application for pre-mix compositions are of WS, LS, ES, FS, WG or CS-type.
  • compositions in question give, after two-to-tenfold dilution, active components concentrations of from 0.01 to 60% by weight, preferably from 0.1 to 40%, in the ready-to-use preparations. Application can be carried out before or during sowing.
  • Methods for applying or treating compound I and compound II and compositions thereof, respectively, on to plant propagation material, especially seeds include dressing, coating, pelleting, dusting, soaking and in-furrow application methods of the propagation material.
  • compound I and compound II or the compositions thereof, respectively are applied on to the plant propagation material by a method such that germination is not induced, e. g. by seed dressing, pelleting, coating and dusting.
  • the plant propagation material is a seed, seed piece (i.e. stalk) or seed bulb.
  • the present method can be applied to a seed in any physiological state, it is preferred that the seed be in a sufficiently durable state that it incurs no damage during the treatment process.
  • the seed would be a seed that had been harvested from the field; removed from the plant; and separated from any cob, stalk, outer husk, and surrounding pulp or other non-seed plant material.
  • the seed would preferably also be biologically stable to the extent that the treatment would cause no biological damage to the seed. It is believed that the treatment can be applied to the seed at any time between harvest of the seed and sowing of the seed or during the sowing process (seed directed applications).
  • the seed may also be primed either before or after the treatment.
  • Treatment could vary from a thin film (dressing) of the formulation containing the combination, for example, a mixture of active ingredient(s), on a plant propagation material, such as a seed, where the original size and/or shape are recognizable to an intermediary state (such as a coating) and then to a thicker film (such as pelleting with many layers of different materials (such as carriers, for example, clays; different formulations, such as of other active ingredients; polymers; and colourants) where the original shape and/or size of the seed is no longer recognizable.
  • a thin film dressing
  • the formulation containing the combination for example, a mixture of active ingredient(s)
  • a plant propagation material such as a seed
  • a thicker film such as pelleting with many layers of different materials (such as carriers, for example, clays; different formulations, such as of other active ingredients; polymers; and colourants) where the original shape and/or size of the seed is no longer recognizable.
  • Seed can be treated by applying thereto the component 1) and component 2) present in the inventive mixtures in any desired sequence or simultaneously.
  • the seed treatment occurs to an unsown seed, and the term “unsown seed” is meant to include seed at any period between the harvest of the seed and the sowing of the seed in the ground for the purpose of germination and growth of the plant. Treatment to an unsown seed is not meant to include those practices in which the active ingredient is applied to the soil but would include any application practice that would target the seed during the planting process.
  • the treatment occurs before sowing of the seed so that the sown seed has been pre-treated with the combination.
  • seed coating or seed pelleting are preferred in the treatment of the combinations according to the invention.
  • the ingredients in each combination are adhered on to the seed and therefore available for pest control.
  • the treated seeds can be stored, handled, sowed and tilled in the same manner as any other active ingredient treated seed.
  • the present invention relates to a method for protection of plant propagation material from pests and/or improving the health of plants grown from said plant propagation material, wherein the soil, wherein plant propagation material is sown, is treated with an effective amount of an inventive mixture.
  • the present invention relates to a method for protection of plant propagation material from pests, wherein the soil, wherein plant propagation material is sown, is treated with an effective amount of an inventive mixture.
  • the present invention relates to a method for protection of plant propagation material from harmful fungi, wherein the soil, wherein plant propagation material is sown, is treated with an effective amount of an inventive mixture.
  • the present invention relates to a method for protection of plant propagation material from animal pests (insects, acarids or nematodes), wherein the soil, wherein plant propagation material is sown, is treated with an effective amount of an inventive mixture.
  • the total amounts of active components applied are, depending on the kind of effect desired, from 0.001 to 10 kg per ha, preferably from 0.005 to 2 kg per ha, more preferably from 0.05 to 0.9 kg per ha, in particular from 0.1 to 0.75 kg per ha.
  • the application rates for foliar or soil (e. g. in furrow) application methods preferably range from about 1 ⁇ 10 6 to 5 ⁇ 10 15 (or more) CFU/ha and even more preferably, the spore concentration is about 1 ⁇ 10 7 to about 1 ⁇ 10 12 CFU/ha.
  • the amount of the inventive mixtures is in the range from 0.01-10 kg, preferably from 0.1-1000 g, more preferably from 1-100 g per 100 kilogram of plant propagation material (preferably seeds).
  • the application rates with respect to plant propagation material preferably range from about 1 ⁇ 10 6 to 1 ⁇ 10 12 (or more) CFU/seed.
  • the concentration is about 1 ⁇ 10 6 to about 1 ⁇ 10 11 CFU/seed.
  • the application rates with respect to plant propagation material also preferably range from about 1 ⁇ 10 7 to 1 ⁇ 10 14 (or more) CFU per 100 kg of seed, preferably from 1 ⁇ 10 9 to about 1 ⁇ 10 11 CFU per 100 kg of seed.
  • the amount of active components applied depends on the kind of application area and on the desired effect. Amounts customarily applied in the protection of materials are 0.001 g to 2 kg, preferably 0.005 g to 1 kg, of active components per cubic meter of treated material.
  • the user applies the composition according to the invention usually from a predosage device, a knapsack sprayer, a spray tank, a spray plane, or an irrigation system.
  • the agrochemical composition is made up with water, buffer, and/or further auxiliaries to the desired application concentration and the ready-to-use spray liquor or the agrochemical composition according to the invention is thus obtained.
  • 20 to 2000 liters, preferably 50 to 400 liters, of the ready-to-use spray liquor are applied per hectare of agricultural useful area.
  • the compound ratios are advantageously chosen so as to produce a synergistic effect.
  • the solid material (dry matter) of the biopesticides (with the exception of oils such as Neem oil, Tagetes oil, etc.) are considered as active components (e.g. to be obtained after drying or evaporation of the extraction medium or filtration of the suspension medium in case of liquid formulations of the microbial pesticides).
  • the weight ratios and percentages used herein for a biological extract such as Quillay extract are based on the total weight of the dry content (solid material) of the respective extract(s).
  • the total weight ratios of compositions comprising at least one microbial pesticide in the form of viable microbial cells including dormant forms can be determined using the amount of CFU of the respective microorganism to calclulate the total weight of the respective active component with the following equation that 1 ⁇ 10 10 CFU equals one gram of total weight of the respective active component.
  • Colony forming unit is a measure of viable microbial cells, in particular fungal and bacterial cells.
  • CFU may also be understood as the number of (juvenile) individual nematodes in case of (entomopathogenic) nematode biopesticides, such as Steinernema feltiae.
  • the weight ratio of the component 1) and the component 2) generally depends from the properties of the active components used, usually it is in the range of from 1:10,000 to 10,000:1, regularly in the range of from 1:100 to 10,000:1, preferably in the range of from 1:100 to 5,000:1, more preferably in the range of from 1:1 to 1,000:1, even more preferably in the range of from 1:1 to 500:1 and in particular in the range of from 10:1 to 300:1.
  • the weight ratio of the component 1) and the component 2) usually is in the range of from 20,000:1 to 1:10, often in the range of from 10,000:1 to 1:1, regularly in the range of from 5,000:1 to 5:1, preferably in the range of from 5,000:1 to 10:1, more preferably in the range of from 2,000:1 to 30:1, even more preferably in the range of from 2,000:1 to 100:1 and in particular in the range of from 1,000:1 to 100:1.
  • the weight ratio of the component 1) and the component 2) usually is in the range of from 20,000:1 to 1:1,000, often in the range of from 10,000:1 to 1:100, regularly in the range of from 5,000:1 to 1:1, preferably in the range of from 5,000:1 to 10:1, more preferably in the range of from 2,000:1 to 30:1, even more preferably in the range of from 2,000:1 to 100:1 and in particular in the range of from 1,000:1 to 100:1.
  • the weight ratio of the component 1) and the component 2) usually is in the range of from 1:1 to 1:1000, often in the range of from 1:1 to 1:100, regularly in the range of from 1:1 to 1:50, preferably in the range of from 1:1 to 1:20, more preferably in the range of from 1:1 to 1:10, even more preferably in the range of from 1:1 to 1:4 and in particular in the range of from 1:1 to 1:2.
  • the total weight of component 1) is calculated on the basis of of the amount of CFU of component 1), wherein 1 ⁇ 10 10 CFU equals one gram of total weight of component 1); and the total weight of component 2) in case of microorganisms is calculated on the basis of of the amount of CFU of component 2), wherein 1 ⁇ 10 10 CFU equals one gram of total weight of component 2).
  • the chemical pesticides II were formulated separately as a stock solution having a concentration of 10000 ppm in dimethyl sulfoxide.
  • the stock solutions of the chemical pesticides II were mixed according to the ratio, diluted to the stated concentrations and pipetted onto a filter micro titer plate (MTP).
  • MTP filter micro titer plate
  • a spore suspension of the pathogen e.g. Botrytis cinerea, Septoria tritici , etc.
  • aqueous biomalt solution was added as well as different concentrations of spores or cells of microorganisms I and, if applicable, the microbial pesticides II.
  • the measured parameters were compared to the growth of the active compound-free control variant (100%) and the fungus-free and active compound-free blank value to determine the relative growth in % of the pathogens in the respective active compounds.

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