US10214768B2 - Coumarin compounds and their uses as fluorescent labels - Google Patents

Coumarin compounds and their uses as fluorescent labels Download PDF

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US10214768B2
US10214768B2 US15/851,014 US201715851014A US10214768B2 US 10214768 B2 US10214768 B2 US 10214768B2 US 201715851014 A US201715851014 A US 201715851014A US 10214768 B2 US10214768 B2 US 10214768B2
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Nikolai Romanov
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Illumina Cambridge Ltd
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    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence

Definitions

  • the present application relates to coumarin compounds.
  • the compounds may be used as fluorescent labels, particularly for nucleotide labeling in nucleic acid sequencing applications.
  • Non-radioactive detection of nucleic acids utilizing fluorescent labels is an important technology in molecular biology. Many procedures employed in recombinant DNA technology previously relied on the use of nucleotides or polynucleotides radioactively labeled with, for example 32 P. Radioactive compounds permit sensitive detection of nucleic acids and other molecules of interest. However, there are serious limitations in the use of radioactive isotopes such as their expense, limited shelf life and more importantly safety considerations. Eliminating the need for radioactive labels enhances safety whilst reducing the environmental impact and costs associated with, for example, reagent disposal. Methods amenable to non-radioactive fluorescent detection include by way of non-limiting example, automated DNA sequencing, hybridization methods, real-time detection of polymerase-chain-reaction products and immunoassays.
  • multiple spectrally distinguishable fluorescent labels in order to achieve independent detection of a plurality of spatially overlapping analytes.
  • the number of reaction vessels may be reduced to simplify experimental protocols and facilitate the production of application-specific reagent kits.
  • multiplex fluorescent detection allows for the analysis of several different nucleotide bases in a single electrophoresis lane. This increases throughput over detection systems using a single-color, and also can reduce the uncertainties associated with inter-lane electrophoretic mobility variations.
  • multiplex fluorescent detection can be problematic and there are a number of important factors, which constrain selection of fluorescent labels.
  • simultaneous excitation may be difficult because the absorption bands of the dyes for different spectral regions may be widely separated.
  • Many excitation methods use high power lasers and therefore the dye must have sufficient photo-stability to withstand such laser excitation.
  • a final consideration of particular importance in molecular biology methods is that the fluorescent dyes must be compatible with the reagent chemistries.
  • the fluorescent dyes used in DNA synthesis or sequencing reactions must be compatible with the solvents and reagents, buffers, polymerase enzymes and ligase enzymes used in those reactions.
  • PCT Publication No. WO 2007/135368 describes a class of rhodamine compounds used as fluorescent labels.
  • Coumarin dyes family has attracted attention of chemists due to their remarkable spectral properties. Nevertheless, there are only a few photo-stable fluorescent dyes with large Stokes shifts (LSS) that are commercially available. Most of these dyes also contain the coumarin fragment as a scaffold. For example, most of the dyes from Dyomics are coumarin derivatives absorbing at about 480-520 nm, and emitting in the region of 560-630. Other examples of this class of coumarin dyes include phosphorylated coumarin based dyes as disclosed in U.S. Publication No. 2014/0220588 and commercially available dyes Star440SXP and Star 470SXP from Abberior.
  • LSS fluorescent dyes include Pacific OrangeTM (abs. 390 nm, emission 540 nm; Stokes shift 150 nm, Invitrogen) and BD HorizonTM V500 (abs. 415 nm, emission 500 nm; Stokes shift 85 nm, BD Biosciences) ChromeoTM 494 (abs. 494 nm, emission 628 nm, Stokes Shift 134 nm, Active Motive).
  • Described herein are novel coumarin derivatives and their use as bio-molecule labels, particularly as labels for nucleotides used in nucleic acid sequencing.
  • improvements can be seen in the length, intensity and quality of sequencing read obtainable due to the use of these new fluorescent compounds.
  • Some embodiments described herein are related to fluorescent compounds of Formula (II) with a Stokes shift at least about 60 nm, salts, or mesomeric forms thereof:
  • Some embodiments described herein are related to nucleotide or oligonucleotide labeled with a compound of Formula (I) or Formula (II).
  • kits containing one or more nucleotides where at least one nucleotide is a labeled nucleotide described herein.
  • Some further embodiments described herein are related to methods of sequencing including incorporating a labeled nucleotide described herein in a sequencing assay, and detecting the labeled nucleotide.
  • FIG. 1 illustrates the usability of the A-nucleotide labeled with the new coumarin dye I-16 as described herein for sequencing analysis.
  • FIG. 2 illustrates the usability of the A-nucleotide labeled with a commercial fluorescent dye DY510XL with long Stokes shift for sequencing analysis.
  • FIG. 3 illustrates the usability of the A-nucleotide labeled with a commercial fluorescent dye Chromeo494 with long Stokes shift for sequencing analysis.
  • Embodiments described herein relate to new coumarin dyes and their derivatives of the structure of Formula (I) for use as fluorescent labels. Further embodiments relate to fluorescent compound of the structure of Formula (II) with a Stokes shift of at least about 60 nm.
  • These new fluorescent dyes may be used as fluorescent labels, particularly for nucleotide labeling in nucleic acid sequencing applications. Methods of preparing these fluorescent dyes and downstream sequencing applications utilizing these dyes are also exemplified.
  • the fluorescence intensities of the new dyes and their bio-conjugates are nearly equal when irradiated with either blue or green light sources.
  • the fluorescence intensities are about the same in some cases. As described below, this property holds true in solution and on flow cells, enabling simplified sequencing analysis with high quality.
  • the alkyl or substituted alkyl disclosed herein is C 1-12 alkyl, or more preferably C 1-6 alkyl.
  • the alkoxy disclosed herein is C 1-12 alkoxy, or more preferably C 1-6 alkoxy.
  • the alkenyl and alkynyl groups disclosed herein are C 2-6 alkenyl and C 2-6 alkynyl.
  • the haloalkyl, haloalkoxy, aminoalkyl, hydroxyalkyl, heteroalkyl groups disclosed herein are C 1-12 haloalkyl, C 1-12 haloalkoxy, C 1-12 aminoalkyl, C 1-12 hydroxyalkyl and C 1-12 heteroalkyl; more preferably C 1-6 haloalkyl, C 1-6 haloalkoxy, C 1-6 aminoalkyl, C 1-6 hydroxyalkyl and C 1-6 heteroalkyl.
  • the alkoxyalkyl group disclosed herein is C 1-6 alkoxy(C 1-6 alkyl).
  • the optionally substituted aryl disclosed herein is optionally substituted C 6-10 aryl, for example, phenyl.
  • the optionally substituted heteroaryl disclosed herein is optionally substituted 5-10 membered heteroaryl; more preferably, optionally substituted 5-6 membered heteroaryl.
  • the optionally substituted carbocyclyl disclosed herein is optionally substituted 3-7 membered carbocyclyl, in particular 3-7 membered cycloalkyl.
  • optionally substituted heterocyclyl disclosed herein are optionally substituted 3-7 membered heterocyclyl, more preferably 5-6 membered heterocyclyl.
  • any of R 2 through R 9 may be selected from an alkyl substituted with one or more substituents selected from carboxyl (—CO 2 H) or carboxylate (CO 2 ⁇ ), sulfo (SO 3 H) or sulfonate (SO 3 ⁇ ) groups.
  • the compounds of Formula (I) are also represented by its salt form Formula (I′) with an organic or inorganic cation:
  • K is an organic or inorganic cation
  • n is an integer selected from 1 to 20.
  • R 1 is
  • X is O. In some other embodiments, X is O. In some such embodiments, the compounds of Formula (I) are also represented by Formula (Ia):
  • R 1 is substituted with one or more substituents selected from the group consisting of alkyl, halo, and C-carboxy. In one embodiment, R 1 is substituted with a chloro (i.e., —Cl). In another embodiment, R 1 is substituted with a carboxyl (i.e., —C(O)OH).
  • R 1 is
  • R 1 is
  • R 10a , R 10b or R 10c when R 10a , R 10b or R 10c is connected to a pyridyl group bearing a positive charge on the nitrogen atom, R 10a , R 10b or R 10c may contain a negative charge so that R 1 as a whole is charge neutral.
  • R 10a , R 10b or R 10c when R 10a , R 10b or R 10c is connected to a pyridyl group bearing a positive charge on the nitrogen atom, the compound described herein may contain a counterion so that the compound as a whole is charge neutral.
  • R 10a , R 10b or R 10c is a substituted alkyl, for example, substituted C 1 , C 2 , C 3 , C 4 , C 5 , or C 6 alkyl.
  • the alkyl is substituted with carboxyl (—CO 2 H), carboxylate (CO 2 ⁇ ), sulfo (SO 3 H), or sulfonate (SO 3 ⁇ ).
  • the compounds of Formula (I) are also represented by Formula (Ib) and (Ic) or their salt Formula (Ib′) and (Ic′):
  • the bond represented by a solid and dashed line is a double bond and the compounds are also presented by Formula (I-1), (I′-1), (Ia-1), (Ib-1), (Ib′-1), (Ic-1), or (Ic′-1):
  • R 2 is alkyl. In one embodiment, R 2 is methyl. In some other embodiments, R 2 is H.
  • R 4 and R 5 is alkyl. In some such embodiments, each R 4 and R 5 is alkyl. In one embodiment, both R 4 and R 5 are methyl. In some alternative embodiments, at least one of R 4 and R 5 is H. In one such embodiment, both R 4 and R 5 are H.
  • the bond represented by a solid and dashed line is a single bond and the compounds are also presented by Formula (I-2), (I′-2), (Ia-2), (Ib-2), (Ib′-2), (Ic-2), or (Ic′-2):
  • R 2 and R 3 is alkyl. In some further embodiments, both R 2 and R 3 are alkyl. In one embodiment, both R 2 and R 3 are methyl. In some other embodiments, at least one of R 2 and R 3 is H. In one embodiment, both R 2 and R 3 are H.
  • R 4 and R 5 is H. In one such embodiment, both R 4 and R 5 are H. In some alternative embodiments, at least one of R 4 and R 5 is alkyl. In some such embodiments, each R 4 and R 5 is alkyl. In one embodiment, both R 4 and R 5 are methyl.
  • R 6 is a substituted alkyl, for example, substituted C 1 , C 2 , C 3 , C 4 , C 5 , or C 6 alkyl. In one embodiment, R 6 is alkyl substituted with carboxyl.
  • R 6 is an alkyl substituted with —C(O)OR 12 , and wherein R 12 is selected from the group consisting of optionally substituted alkyl, optionally substituted aryl, optionally substituted heteroaryl, and optionally substituted 3 to 7 membered cycloalkyl.
  • R 12 is an alkyl, for example, methyl, ethyl, or t-butyl.
  • R 6 is an alkyl substituted with —C(O)NR 13 R 14 , and wherein each R 13 and R 14 is independently selected from H, optionally substituted alkyl, optionally substituted aryl, optionally substituted heteroaryl and optionally substituted 3 to 7 membered cycloalkyl. In some further embodiments, R 13 and R 14 is independently selected from an alkyl substituted with one or more substituents selected from the group consisting of carboxyl, carboxylate, —C(O)OR 11 , sulfo and sulfonate.
  • R 7 is H.
  • R 6 and R 7 are joined together with the atoms to which they are attached to form an optionally substituted 3 to 10 membered heterocyclyl, for example, an optionally substituted 6 membered heterocyclyl.
  • the optionally substituted heterocyclyl contains one heteroatom.
  • the optionally substituted heterocyclyl is substituted with one or more alkyls, for example, methyl.
  • R 8 is H.
  • R 9 is H.
  • exemplary compounds of Formula (I) include Compounds I-1 through I-20 and Compounds I-22 through I-32 as shown in Table 1 below:
  • the compound is covalently attached to a nucleotide or oligonucleotide via R 6 , and wherein R 6 is a substituted alkyl, for example, a substituted C 1 , C 2 , C 3 , C 4 , C 5 , or C 6 alkyl. In one embodiment, R 6 is an alkyl substituted with carboxyl.
  • the compound is covalently attached to a nucleotide or oligonucleotide via R 8 , and wherein R 8 is a substituted alkyl, for example, a substituted C 1 , C 2 , C 3 , C 4 , C 5 , or C 6 alkyl. In one embodiment, R 8 is an alkyl substituted with carboxyl.
  • the compound is covalently attached to a nucleotide or oligonucleotide via R 10a , R 10b , or R 10c , and wherein each of R 10a , R 10b , or R 10C is a substituted alkyl, for example, a substituted C 1 , C 2 , C 3 , C 4 , C 5 , or C 6 alkyl. In one embodiment, each of R 10a , R 10b , or R 10C is an alkyl substituted with carboxyl.
  • the structure of compound of Formula (I) is represented in one or more mesomeric forms:
  • Some embodiments described herein are related to fluorescent compounds of Formula (II) with a Stokes shift at least about 60 nm, or salts, mesomeric forms thereof:
  • the fluorescent compounds of Formula (II) have a Stokes shift ranging from about 60 nm to about 100 nm, or from about 60 nm to about 90 nm.
  • the alkyl or substituted alkyl disclosed herein is C 1-12 alkyl, or more preferably C 1-6 alkyl.
  • the alkoxy disclosed herein is C 1-12 alkoxy, or more preferably C 1-6 alkoxy.
  • the alkenyl and alkynyl groups disclosed herein are C 2-6 alkenyl and C 2-6 alkynyl.
  • the haloalkyl, haloalkoxy, aminoalkyl, hydroxyalkyl, heteroalkyl groups disclosed herein are C 1-12 haloalkyl, C 1-12 haloalkoxy, C 1-12 aminoalkyl, C 1-12 hydroxyalkyl and C 1-12 heteroalkyl; more preferably C 1-6 haloalkyl, C 1-6 haloalkoxy, C 1-6 aminoalkyl, C 1-6 hydroxyalkyl and C 1-6 heteroalkyl.
  • the alkoxyalkyl group disclosed herein is C 1-6 alkoxy(C 1-6 alkyl).
  • the optionally substituted aryl disclosed herein is optionally substituted C 6-10 aryl, for example, phenyl.
  • the optionally substituted heteroaryl disclosed herein is optionally substituted 5-10 membered heteroaryl; more preferably, optionally substituted 5-6 membered heteroaryl.
  • the optionally substituted carbocyclyl disclosed herein is optionally substituted 3-7 membered carbocyclyl, in particular 3-7 membered cycloalkyl.
  • optionally substituted heterocyclyl disclosed herein are optionally substituted 3-7 membered heterocyclyl, more preferably 5-6 membered heterocyclyl.
  • any of R 1 through R 8 may be selected from an alkyl substituted with one or more substituents selected from carboxyl (—CO 2 H) or carboxylate (CO 2 ⁇ ), sulfo (SO 3 H) or sulfonate (SO 3 ⁇ ) groups.
  • the substituted alkyl is a substituted C 1 , C 2 , C 3 , C 4 , C 5 , or C 6 alkyl.
  • the compounds of Formula (II) are also represented by its salt form Formula (II′) with an organic or inorganic cation:
  • K is an organic or inorganic cation
  • n is an integer selected from 1 to 20.
  • R Het is a 9 membered heteroaryl optionally substituted with one or more R 10 .
  • R Het is selected from optionally substituted benzothiazolyl or benzoxazolyl, for example, 2-benzothiazolyl or 2-benzoxazolyl.
  • R Het is a 6 membered heteroaryl optionally substituted with one or more R 10 .
  • R Het is an substituted pyridyl, for example, 4-pyridyl with the structure:
  • R 10 when R 10 is connected to a pyridyl group bearing a positive charge on the nitrogen atom, R 10 may contain a negative charge so that R Het as a whole is charge neutral.
  • the compound described herein when R 10 is connected to a pyridyl group bearing a positive charge on the nitrogen atom, the compound described herein may contain a counterion so that the compound as a whole is charge neutral.
  • R 10 is a substituted alkyl, for example, substituted C 1 , C 2 , C 3 , C 4 , C 5 , or C 6 alkyl.
  • R 10 is substituted with carboxyl (—CO 2 H), carboxylate (CO 2 ⁇ ), sulfo (SO 3 H), or sulfonate (SO 3 ⁇ ).
  • the bond represented by a solid and dashed line in Formula (II) is a double bond and the compounds are also represented by Formula (II-1) or (II′-1):
  • R 2 is alkyl. In one embodiment, R 2 is methyl. In some other embodiments, R 2 is H.
  • R 4 and R 5 is alkyl. In some such embodiments, each R 4 and R 5 is alkyl. In one embodiment, both R 4 and R 5 are methyl. In some alternative embodiments, at least one of R 4 and R 5 is H. In one such embodiment, both R 4 and R 5 are H.
  • the bond represented by a solid and dashed line is a single bond and the compounds are also presented by Formula (II-2) or (II′-2):
  • R 2 and R 3 is alkyl. In some further embodiments, both R 2 and R 3 are alkyl. In one embodiment, both R 2 and R 3 are methyl. In some other embodiments, at least one of R 2 and R 3 is H. In one embodiment, both R 2 and R 3 are H.
  • R 4 and R 5 is H. In one such embodiment, both R 4 and R 5 are H. In some alternative embodiments, at least one of R 4 and R 5 is alkyl. In some such embodiments, each R 4 and R 5 is alkyl. In one embodiment, both R 4 and R 5 are methyl.
  • R 6 is a substituted alkyl, for example, substituted C 1 , C 2 , C 3 , C 4 , C 5 , or C 6 alkyl.
  • R 6 is alkyl substituted with carboxyl.
  • R 6 is an alkyl substituted with —C(O)OR 12 , and wherein R 12 is selected from the group consisting of optionally substituted alkyl, optionally substituted aryl, optionally substituted heteroaryl, and optionally substituted 3 to 7 membered cycloalkyl.
  • R 12 is an alkyl, for example, methyl, ethyl, or t-butyl.
  • R 6 is an alkyl substituted with —C(O)NR 13 R 14 , and wherein each R 13 and R 14 is independently selected from H, optionally substituted alkyl, optionally substituted aryl, optionally substituted heteroaryl and optionally substituted 3 to 7 membered cycloalkyl.
  • R 13 and R 14 is independently selected from an alkyl substituted with one or more substituents selected from the group consisting of carboxyl, carboxylate, —C(O)OR 11 , sulfo and sulfonate.
  • R 7 is H.
  • R 6 and R 7 are joined together with the atoms to which they are attached to form an optionally substituted 3 to 10 membered heterocyclyl, for example, an optionally substituted 6 membered heterocyclyl.
  • the optionally substituted heterocyclyl contains one heteroatom.
  • the optionally substituted heterocyclyl is substituted with one or more alkyl, for example, methyl.
  • R 1 is H.
  • R 8 is H.
  • a compound of Formula (I) or (II) when a compound of Formula (I) or (II) contains positively or negatively charged substituent groups, it may also contains a negatively or positively charged counterion such that the compound as a whole is neutral.
  • the above terms are to be interpreted synonymously with the phrases “having at least” or “including at least.”
  • the term “comprising” means that the process includes at least the recited steps, but may include additional steps.
  • the term “comprising” means that the compound, composition, or device includes at least the recited features or components, but may also include additional features or components.
  • covalently attached or “covalently bonded” refers to the forming of a chemical bonding that is characterized by the sharing of pairs of electrons between atoms.
  • a covalently attached polymer coating refers to a polymer coating that forms chemical bonds with a functionalized surface of a substrate, as compared to attachment to the surface via other means, for example, adhesion or electrostatic interaction. It will be appreciated that polymers that are attached covalently to a surface can also be bonded via means in addition to covalent attachment.
  • halogen or “halo,” as used herein, means any one of the radio-stable atoms of column 7 of the Periodic Table of the Elements, e.g., fluorine, chlorine, bromine, or iodine, with fluorine and chlorine being preferred.
  • alkyl refers to a straight or branched hydrocarbon chain that is fully saturated (i.e., contains no double or triple bonds).
  • the alkyl group may have 1 to 20 carbon atoms (whenever it appears herein, a numerical range such as “1 to 20” refers to each integer in the given range, e.g., “1 to 20 carbon atoms” means that the alkyl group may consist of 1 carbon atom, 2 carbon atoms, 3 carbon atoms, etc., up to and including 20 carbon atoms, although the present definition also covers the occurrence of the term “alkyl” where no numerical range is designated).
  • the alkyl group may also be a medium size alkyl having 1 to 9 carbon atoms.
  • the alkyl group could also be a lower alkyl having 1 to 6 carbon atoms.
  • the alkyl group may be designated as “C 1-4 alkyl” or similar designations.
  • C 1-6 alkyl indicates that there are one to six carbon atoms in the alkyl chain, i.e., the alkyl chain is selected from the group consisting of methyl, ethyl, propyl, iso-propyl, n-butyl, iso-butyl, sec-butyl, and t-butyl.
  • Typical alkyl groups include, but are in no way limited to, methyl, ethyl, propyl, isopropyl, butyl, isobutyl, tertiary butyl, pentyl, hexyl, and the like.
  • alkoxy refers to the formula —OR wherein R is an alkyl as is defined above, such as “C 1-9 alkoxy”, including but not limited to methoxy, ethoxy, n-propoxy, 1-methylethoxy (isopropoxy), n-butoxy, iso-butoxy, sec-butoxy, and tert-butoxy, and the like.
  • alkenyl refers to a straight or branched hydrocarbon chain containing one or more double bonds.
  • the alkenyl group may have 2 to 20 carbon atoms, although the present definition also covers the occurrence of the term “alkenyl” where no numerical range is designated.
  • the alkenyl group may also be a medium size alkenyl having 2 to 9 carbon atoms.
  • the alkenyl group could also be a lower alkenyl having 2 to 6 carbon atoms.
  • the alkenyl group may be designated as “C 2-6 alkenyl” or similar designations.
  • C 2-6 alkenyl indicates that there are two to six carbon atoms in the alkenyl chain, i.e., the alkenyl chain is selected from the group consisting of ethenyl, propen-1-yl, propen-2-yl, propen-3-yl, buten-1-yl, buten-2-yl, buten-3-yl, buten-4-yl, 1-methyl-propen-1-yl, 2-methyl-propen-1-yl, 1-ethyl-ethen-1-yl, 2-methyl-propen-3-yl, buta-1,3-dienyl, buta-1,2,-dienyl, and buta-1,2-dien-4-yl.
  • Typical alkenyl groups include, but are in no way limited to, ethenyl, propenyl, butenyl, pentenyl, and hexenyl, and the like.
  • alkynyl refers to a straight or branched hydrocarbon chain containing one or more triple bonds.
  • the alkynyl group may have 2 to 20 carbon atoms, although the present definition also covers the occurrence of the term “alkynyl” where no numerical range is designated.
  • the alkynyl group may also be a medium size alkynyl having 2 to 9 carbon atoms.
  • the alkynyl group could also be a lower alkynyl having 2 to 6 carbon atoms.
  • the alkynyl group may be designated as “C 2-6 alkynyl” or similar designations.
  • C 2-6 alkynyl indicates that there are two to six carbon atoms in the alkynyl chain, i.e., the alkynyl chain is selected from the group consisting of ethynyl, propyn-1-yl, propyn-2-yl, butyn-1-yl, butyn-3-yl, butyn-4-yl, and 2-butynyl.
  • Typical alkynyl groups include, but are in no way limited to, ethynyl, propynyl, butynyl, pentynyl, and hexynyl, and the like.
  • heteroalkyl refers to a straight or branched hydrocarbon chain containing one or more heteroatoms, that is, an element other than carbon, including but not limited to, nitrogen, oxygen and sulfur, in the chain backbone.
  • the heteroalkyl group may have 1 to 20 carbon atom, although the present definition also covers the occurrence of the term “heteroalkyl” where no numerical range is designated.
  • the heteroalkyl group may also be a medium size heteroalkyl having 1 to 9 carbon atoms.
  • the heteroalkyl group could also be a lower heteroalkyl having 1 to 6 carbon atoms.
  • the heteroalkyl group may be designated as “C 1-6 heteroalkyl” or similar designations.
  • the heteroalkyl group may contain one or more heteroatoms.
  • C 1-6 heteroalkyl indicates that there are one to six carbon atoms in the heteroalkyl chain and additionally one or more heteroatoms in the backbone of the chain.
  • aromatic refers to a ring or ring system having a conjugated pi electron system and includes both carbocyclic aromatic (e.g., phenyl) and heterocyclic aromatic groups (e.g., pyridine).
  • carbocyclic aromatic e.g., phenyl
  • heterocyclic aromatic groups e.g., pyridine
  • the term includes monocyclic or fused-ring polycyclic (i.e., rings which share adjacent pairs of atoms) groups provided that the entire ring system is aromatic.
  • aryl refers to an aromatic ring or ring system (i.e., two or more fused rings that share two adjacent carbon atoms) containing only carbon in the ring backbone. When the aryl is a ring system, every ring in the system is aromatic.
  • the aryl group may have 6 to 18 carbon atoms, although the present definition also covers the occurrence of the term “aryl” where no numerical range is designated. In some embodiments, the aryl group has 6 to 10 carbon atoms.
  • the aryl group may be designated as “C 6-10 aryl,” “C 6 or C 10 aryl,” or similar designations. Examples of aryl groups include, but are not limited to, phenyl, naphthyl, azulenyl, and anthracenyl.
  • an “aralkyl” or “arylalkyl” is an aryl group connected, as a substituent, via an alkylene group, such as “C 7-14 aralkyl” and the like, including but not limited to benzyl, 2-phenylethyl, 3-phenylpropyl, and naphthylalkyl.
  • the alkylene group is a lower alkylene group (i.e., a C 1-6 alkylene group).
  • heteroaryl refers to an aromatic ring or ring system (i.e., two or more fused rings that share two adjacent atoms) that contain(s) one or more heteroatoms, that is, an element other than carbon, including but not limited to, nitrogen, oxygen and sulfur, in the ring backbone.
  • heteroaryl is a ring system, every ring in the system is aromatic.
  • the heteroaryl group may have 5-18 ring members (i.e., the number of atoms making up the ring backbone, including carbon atoms and heteroatoms), although the present definition also covers the occurrence of the term “heteroaryl” where no numerical range is designated.
  • the heteroaryl group has 5 to 10 ring members or 5 to 7 ring members.
  • the heteroaryl group may be designated as “5-7 membered heteroaryl,” “5-10 membered heteroaryl,” or similar designations.
  • heteroaryl rings include, but are not limited to, furyl, thienyl, phthalazinyl, pyrrolyl, oxazolyl, thiazolyl, imidazolyl, pyrazolyl, isoxazolyl, isothiazolyl, triazolyl, thiadiazolyl, pyridinyl, pyridazinyl, pyrimidinyl, pyrazinyl, triazinyl, quinolinyl, isoquinlinyl, benzimidazolyl, benzoxazolyl, benzothiazolyl, indolyl, isoindolyl, and benzothienyl.
  • heteroarylkyl or “heteroarylalkyl” is heteroaryl group connected, as a substituent, via an alkylene group. Examples include but are not limited to 2-thienylmethyl, 3-thienylmethyl, furylmethyl, thienylethyl, pyrrolylalkyl, pyridylalkyl, isoxazollylalkyl, and imidazolylalkyl.
  • the alkylene group is a lower alkylene group (i.e., a C 1-6 alkylene group).
  • carbocyclyl means a non-aromatic cyclic ring or ring system containing only carbon atoms in the ring system backbone. When the carbocyclyl is a ring system, two or more rings may be joined together in a fused, bridged or spiro-connected fashion. Carbocyclyls may have any degree of saturation provided that at least one ring in a ring system is not aromatic. Thus, carbocyclyls include cycloalkyls, cycloalkenyls, and cycloalkynyls.
  • the carbocyclyl group may have 3 to 20 carbon atoms, although the present definition also covers the occurrence of the term “carbocyclyl” where no numerical range is designated.
  • the carbocyclyl group may also be a medium size carbocyclyl having 3 to 10 carbon atoms.
  • the carbocyclyl group could also be a carbocyclyl having 3 to 6 carbon atoms.
  • the carbocyclyl group may be designated as “C 3-6 carbocyclyl” or similar designations.
  • carbocyclyl rings include, but are not limited to, cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cyclohexenyl, 2,3-dihydro-indene, bicycle[2.2.2]octanyl, adamantyl, and spiro[4.4]nonanyl.
  • cycloalkyl means a fully saturated carbocyclyl ring or ring system. Examples include cyclopropyl, cyclobutyl, cyclopentyl, and cyclohexyl.
  • heterocyclyl means a non-aromatic cyclic ring or ring system containing at least one heteroatom in the ring backbone. Heterocyclyls may be joined together in a fused, bridged or spiro-connected fashion. Heterocyclyls may have any degree of saturation provided that at least one ring in the ring system is not aromatic. The heteroatom(s) may be present in either a non-aromatic or aromatic ring in the ring system.
  • the heterocyclyl group may have 3 to 20 ring members (i.e., the number of atoms making up the ring backbone, including carbon atoms and heteroatoms), although the present definition also covers the occurrence of the term “heterocyclyl” where no numerical range is designated.
  • the heterocyclyl group may also be a medium size heterocyclyl having 3 to 10 ring members.
  • the heterocyclyl group could also be a heterocyclyl having 3 to 6 ring members.
  • the heterocyclyl group may be designated as “3-6 membered heterocyclyl” or similar designations.
  • the heteroatom(s) are selected from one up to three of O, N or S, and in preferred five membered monocyclic heterocyclyls, the heteroatom(s) are selected from one or two heteroatoms selected from O, N, or S.
  • heterocyclyl rings include, but are not limited to, azepinyl, acridinyl, carbazolyl, cinnolinyl, dioxolanyl, imidazolinyl, imidazolidinyl, morpholinyl, oxiranyl, oxepanyl, thiepanyl, piperidinyl, piperazinyl, dioxopiperazinyl, pyrrolidinyl, pyrrolidonyl, pyrrolidionyl, 4-piperidonyl, pyrazolinyl, pyrazolidinyl, 1,3-dioxinyl, 1,3-dioxanyl, 1,4-dioxinyl, 1,4-dioxanyl, 1,3-oxathianyl, 1,4-oxathiinyl, 1,4-oxathianyl, 2H-1,2-oxazinyl, trioxanyl, hexahydr
  • An “O-carboxy” group refers to a “—OC( ⁇ O)R” group in which R is selected from hydrogen, C 1-6 alkyl, C 2-6 alkenyl, C 2-6 alkynyl, C 3-7 carbocyclyl, C 6-10 aryl, 5-10 membered heteroaryl, and 3-10 membered heterocyclyl, as defined herein.
  • C-carboxy refers to a “—C( ⁇ O)OR” group in which R is selected from hydrogen, C 1-6 alkyl, C 2-6 alkenyl, C 2-6 alkynyl, C 3-7 carbocyclyl, C 6-10 aryl, 5-10 membered heteroaryl, and 3-10 membered heterocyclyl, as defined herein.
  • R is selected from hydrogen, C 1-6 alkyl, C 2-6 alkenyl, C 2-6 alkynyl, C 3-7 carbocyclyl, C 6-10 aryl, 5-10 membered heteroaryl, and 3-10 membered heterocyclyl, as defined herein.
  • a non-limiting example includes carboxyl (i.e., —C( ⁇ O)OH).
  • a “cyano” group refers to a “—CN” group.
  • a “sulfonyl” group refers to an “—SO 2 R” group in which R is selected from hydrogen, C 1-6 alkyl, C 2-6 alkenyl, C 2-6 alkynyl, C 3-7 carbocyclyl, C 6-10 aryl, 5-10 membered heteroaryl, and 3-10 membered heterocyclyl, as defined herein.
  • a “sulfo” or “sulfonyl hydroxide” group refers to a “—S( ⁇ O) 2 —OH” group.
  • a “sulfino” group refers to a “—S( ⁇ O)OH” group.
  • a “sulfonate” group refers to —SO 3 ⁇ .
  • S-sulfonamido refers to a “—SO 2 NR A R B ” group in which R A and R B are each independently selected from hydrogen, C 1-6 alkyl, C 2-6 alkenyl, C 2-6 alkynyl, C 3-7 carbocyclyl, C 6-10 aryl, 5-10 membered heteroaryl, and 3-10 membered heterocyclyl, as defined herein.
  • N-sulfonamido refers to a “—N(R A )SO 2 R B ” group in which R A and R b , are each independently selected from hydrogen, C 1-6 alkyl, C 2-6 alkenyl, C 2-6 alkynyl, C 3-7 carbocyclyl, C 6-10 aryl, 5-10 membered heteroaryl, and 3-10 membered heterocyclyl, as defined herein.
  • a “C-amido” group refers to a “—C( ⁇ O)NR A R B ” group in which R A and R B are each independently selected from hydrogen, C 1-6 alkyl, C 2-6 alkenyl, C 2-6 alkynyl, C 3-7 carbocyclyl, C 6-10 aryl, 5-10 membered heteroaryl, and 3-10 membered heterocyclyl, as defined herein.
  • N-amido refers to a “—N(R A )C( ⁇ O)R B ” group in which R A and R B are each independently selected from hydrogen, C 1-6 alkyl, C 2-6 alkenyl, C 2-6 alkynyl, C 3-7 carbocyclyl, C 6-10 aryl, 5-10 membered heteroaryl, and 3-10 membered heterocyclyl, as defined herein.
  • amino group refers to a “—NR A R B ” group in which R A and R B are each independently selected from hydrogen, C 1-6 alkyl, C 2-6 alkenyl, C 2-6 alkynyl, C 3-7 carbocyclyl, C 6-10 aryl, 5-10 membered heteroaryl, and 3-10 membered heterocyclyl, as defined herein.
  • R A and R B are each independently selected from hydrogen, C 1-6 alkyl, C 2-6 alkenyl, C 2-6 alkynyl, C 3-7 carbocyclyl, C 6-10 aryl, 5-10 membered heteroaryl, and 3-10 membered heterocyclyl, as defined herein.
  • a non-limiting example includes free amino (i.e., —NH 2 ).
  • aminoalkyl refers to an amino group connected via an alkylene group.
  • alkoxyalkyl refers to an alkoxy group connected via an alkylene group, such as a “C 2-8 alkoxyalkyl” and the like.
  • a substituted group is derived from the unsubstituted parent group in which there has been an exchange of one or more hydrogen atoms for another atom or group.
  • substituents independently selected from C 1 -C 6 alkyl, C 1 -C 6 alkenyl, C 1 -C 6 alkynyl, C 1 -C 6 heteroalkyl, C 3 -C 7 carbocyclyl (optionally substituted with halo, C 1 -C 6 alkyl, C 1 -C 6 alkoxy, C 1 -C 6 haloalkyl, and C 1 -C 6 haloalkoxy), C 3 -C 7 -carbocyclyl-C 1 -C 6 -alkyl (optionally substituted with halo, C 1 -C 6 alkyl, C 1 -C 6 alkoxy, C 1
  • a compound may also contains a negatively or positively charged counterion such that the compound as a whole is neutral.
  • radical naming conventions can include either a mono-radical or a di-radical, depending on the context.
  • a substituent requires two points of attachment to the rest of the molecule, it is understood that the substituent is a di-radical.
  • a substituent identified as alkyl that requires two points of attachment includes di-radicals such as —CH 2 —, —CH 2 CH 2 —, —CH 2 CH(CH 3 )CH 2 —, and the like.
  • Other radical naming conventions clearly indicate that the radical is a di-radical such as “alkylene” or “alkenylene.”
  • R 1 and R 2 are defined as selected from the group consisting of hydrogen and alkyl, or R 1 and R 2 together with the atoms to which they are attached form an aryl or carbocyclyl, it is meant that R 1 and R 2 can be selected from hydrogen or alkyl, or alternatively, the substructure has structure:
  • A is an aryl ring or a carbocyclyl containing the depicted double bond.
  • Substrate moieties can be virtually any molecule or substance to which the fluorescent dyes described herein can be conjugated and, by way of non-limiting example, may include nucleosides, nucleotides, polynucleotides, carbohydrates, ligands, particles, solid surfaces, organic and inorganic polymers and combinations or assemblages thereof, such as chromosomes, nuclei, living cells and the like.
  • the dyes can be conjugated by an optional linker by a variety of means including hydrophobic attraction, ionic attraction and covalent attachment. Particularly the dyes are conjugated to the substrate by covalent attachment. More particularly the covalent attachment is by means of a linker group. In some instances, such labeled nucleotides are also referred to as “modified nucleotides.”
  • a particular useful application of the new fluorescent dyes with long Stokes shift as described herein is for labeling biomolecules, for example, nucleotides or oligonucleotides.
  • Some embodiments of the present application are directed to a nucleotide or oligonucleotide labeled with the new fluorescent compounds as described herein.
  • Fluorescent dye molecules with improved fluorescence properties can improve the speed and accuracy of nucleic acid sequencing.
  • Stokes Shift is a key aspect in the detection of the fluorescence in biological applications. For example, the detection of emitted light can be difficult to distinguish from the excitation light when using fluorophores with absorption and fluorescence max very close to each other (i.e., small Stokes shift), because the excitation and emission wavelengths greatly overlap. In contrast, fluorophores with large Stokes shifts are easy to distinguish because of the greater separation between the excitation and emission wavelengths.
  • the Stokes shift is especially critical in multiplex fluorescence applications, because the emission wavelength of one fluorophore may overlap, and therefore excite, another fluorophore in the same sample.
  • fluorescence signal intensity is especially important when measurements are made in water based biological buffers and/or at higher temperature as fluorescence of most dyes is significantly lower at such conditions.
  • the nature of the base to which a dye is attached also affects the fluorescence maximum, fluorescence intensity and other spectral dye properties.
  • the sequence specific interactions between the fluorescent dye and the nucleobase can be tailored by specific design of the fluorescent dyes. Optimization of the structure of the fluorescent dyes can improve their fluorescent properties and also improve the efficiency of nucleotide incorporation, reduce the level of sequencing errors and decrease the usage of reagents in, and therefore the costs of, nucleic acid sequencing.
  • the attachment to the biomolecules may be via R 6 , R 8 , R 10a , R 10b , R 10c or R 10 moiety of the compound of Formula (I) or Formula (II).
  • R 6 , R 8 , R 10a , R 10b , R 10c or R 10 is a substituted alkyl, for example alkyl substituted with carboxyl (i.e., —CO 2 H) or an activated form of carboxyl group, for example, amide or ester, which may be used for attachment to the amino group of the biomolecules.
  • R 6 , R 8 , R 10a , R 10b , R 10c or R 10 may contain an activated ester or amide residue most suitable for further amide/peptide bond formation.
  • activated ester refers to a carboxyl group derivative which is capable of reacting in mild conditions, for example, with a compound containing an amino group.
  • activated esters include but not limited to p-nitrophenyl, pentafluorophenyl and succinimido esters.
  • the dye compounds may be covalently attached to oligonucleotides or nucleotides via the nucleotide base.
  • the labeled nucleotide or oligonucleotide may have the label attached to the C5 position of a pyrimidine base or the C7 position of a 7-deaza purine base through a linker moiety.
  • the labeled nucleotide or oligonucleotide may also have a 3′-OH blocking group covalently attached to the ribose or deoxyribose sugar of the nucleotide.
  • the dye compounds as disclosed herein may include a reactive linker group at one of the substituent positions for covalent attachment of the compound to another molecule.
  • Reactive linking groups are moieties capable of forming a covalent bond.
  • the linker may be a cleavable linker.
  • Use of the term “cleavable linker” is not meant to imply that the whole linker is required to be removed.
  • the cleavage site can be located at a position on the linker that ensures that part of the linker remains attached to the dye and/or substrate moiety after cleavage.
  • Cleavable linkers may be, by way of non-limiting example, electrophilically cleavable linkers, nucleophilically cleavable linkers, photocleavable linkers, cleavable under reductive conditions (for example disulfide or azide containing linkers), oxidative conditions, cleavable via use of safety-catch linkers and cleavable by elimination mechanisms.
  • electrophilically cleavable linkers nucleophilically cleavable linkers, photocleavable linkers, cleavable under reductive conditions (for example disulfide or azide containing linkers), oxidative conditions, cleavable via use of safety-catch linkers and cleavable by elimination mechanisms.
  • Non-limiting examples of linker groups include those disclosed in PCT Publication No. WO2004/018493 (herein incorporated by reference), which connect the bases of nucleotides to labels such as, for example, the new fluorescent compounds described herein. These linkers may be cleaved using water-soluble phosphines or water-soluble transition metal catalysts formed from a transition metal and at least partially water-soluble ligands. In aqueous solution the latter form at least partially water-soluble transition metal complexes. Additional suitable linkers that may be used include those disclosed in PCT Publication No. WO2004/018493 and WO 2007/020457 (both of which are herein incorporated by references.
  • the linker comprises a spacer group of formula —((CH 2 ) 2 O) n —, wherein n is an integer between 2 and 50, as described in WO 2007/020457.
  • Nucleosides and nucleotides may be labeled at sites on the sugar or nucleobase.
  • a “nucleotide” consists of a nitrogenous base, a sugar, and one or more phosphate groups.
  • the sugar is ribose and in DNA is a deoxyribose, i.e. a sugar lacking a hydroxyl group that is present in ribose.
  • the nitrogenous base is a derivative of purine or pyrimidine.
  • the purines are adenine (A) and guanine (G), and the pyrimidines are cytosine (C) and thymine (T) or in the context of RNA, uracil (U).
  • the C-1 atom of deoxyribose is bonded to N-1 of a pyrimidine or N-9 of a purine.
  • a nucleotide is also a phosphate ester of a nucleoside, with esterification occurring on the hydroxyl group attached to the C-3 or C-5 of the sugar. Nucleotides are usually mono, di- or triphosphates.
  • nucleoside is structurally similar to a nucleotide but is missing the phosphate moieties.
  • An example of a nucleoside analog would be one in which the label is linked to the base and there is no phosphate group attached to the sugar molecule.
  • the base is usually referred to as a purine or pyrimidine, the skilled person will appreciate that derivatives and analogues are available which do not alter the capability of the nucleotide or nucleoside to undergo Watson-Crick base pairing.
  • “Derivative” or “analogue” means a compound or molecule whose core structure is the same as, or closely resembles that of a parent compound but which has a chemical or physical modification, such as, for example, a different or additional side group, which allows the derivative nucleotide or nucleoside to be linked to another molecule.
  • the base may be a deazapurine.
  • the derivatives should be capable of undergoing Watson-Crick pairing.
  • “Derivative” and “analogue” also mean a synthetic nucleotide or nucleoside derivative having modified base moieties and/or modified sugar moieties. Such derivatives and analogues are discussed in, for example, Scheit, Nucleotide analogs (John Wiley & Son, 1980) and Uhlman et al., Chemical Reviews 90:543-584, 1990. Nucleotide analogues can also comprise modified phosphodiester linkages including phosphorothioate, phosphorodithioate, alkyl-phosphonate, phosphoranilidate, phosphoramidate linkages and the like.
  • the dye may be attached to any position on the nucleotide base, through a linker, provided that Watson-Crick base pairing can still be carried out.
  • Particular nucleobase labeling sites include the C5 position of a pyrimidine base or the C7 position of a 7-deaza purine base.
  • a linker group may be used to covalently attach a dye to the nucleoside or nucleotide.
  • the labeled nucleoside or nucleotide may be enzymatically incorporable and enzymatically extendable.
  • a linker moiety may be of sufficient length to connect the nucleotide to the compound such that the compound does not significantly interfere with the overall binding and recognition of the nucleotide by a nucleic acid replication enzyme.
  • the linker can also comprise a spacer unit. The spacer distances, for example, the nucleotide base from a cleavage site or label.
  • the blocking group is separate and independent of the dye compound, i.e. not attached to it.
  • the dye may comprise all or part of the 3′-OH blocking group.
  • R′′ can be a 3′-OH blocking group which may or may not comprise the dye compound.
  • there is no blocking group on the 3′ carbon of the pentose sugar and the dye (or dye and linker construct) attached to the base can be of a size or structure sufficient to act as a block to the incorporation of a further nucleotide from a point other than the 3′ site.
  • the block can be due to steric hindrance or can be due to a combination of size, charge and structure.
  • blocking group allows polymerization to be controlled, such as by stopping extension when a modified nucleotide is incorporated. If the blocking effect is reversible, for example by way of non-limiting example by changing chemical conditions or by removal of a chemical block, extension can be stopped at certain points and then allowed to continue.
  • 3′-OH blocking groups include those disclosed in WO 2004/018497 and WO2014/139596, which are hereby incorporated by references.
  • the blocking group may be azidomethyl (—CH 2 N 3 ) or substituted azidomethyl (e.g., —CH(CHF 2 )N 3 or CH(CH 2 F)N 3 ), or allyl.
  • linker and blocking group are both present and are separate moieties which are both cleavable under substantially similar conditions.
  • deprotection and deblocking processes may be more efficient since only a single treatment will be required to remove both the dye compound and the blocking group.
  • polynucleotides incorporating dye compounds described herein.
  • Such polynucleotides may be DNA or RNA comprised respectively of deoxyribonucleotides or ribonucleotides joined in phosphodiester linkage.
  • Polynucleotides may comprise naturally occurring nucleotides, non-naturally occurring (or modified) nucleotides other than the labeled nucleotides described herein or any combination thereof, provided that at least one nucleotide labeled with a dye compound, according to the present application is present.
  • Polynucleotides may also include non-natural backbone linkages and/or non-nucleotide chemical modifications. Chimeric structures comprised of mixtures of ribonucleotides and deoxyribonucleotides comprising at least one labeled nucleotide are also contemplated.
  • non-limiting exemplary fluorescent dye conjugates are shown below:
  • kits including nucleosides and/or nucleotides labeled with the new fluorescent dyes described herein.
  • Such kits will generally include at least one nucleotide or nucleoside labeled with a dye together with at least one further component.
  • the further component(s) may be further modified or unmodified nucleotides or nucleosides.
  • nucleotides labeled with dyes may be supplied in combination with unlabeled or native nucleotides, and/or with fluorescently labeled nucleotides or any combination thereof.
  • Combinations of nucleotides may be provided as separate individual components or as nucleotide mixtures.
  • kits comprise one or more nucleotides wherein at least one nucleotide is a nucleotide labeled with a new fluorescent compound described herein.
  • the kits may comprise two or more labeled nucleotides.
  • the nucleotides may be labeled with two or more fluorescent labels. Two or more of the labels may be excited using a single excitation source, which may be a laser.
  • kits may contain four nucleotides, where the first of four nucleotides is labeled with a compound as disclosed herein, and the second, third, and fourth nucleotides are each may be labeled with a different compound, wherein each compound has a distinct fluorescence maximum and each of the compounds is distinguishable from the other three compounds.
  • the kits may be such that two or more of the compounds have a similar absorbance maximum but different Stokes shift.
  • the fluorescent dye compounds, labeled nucleotides or kits described herein may be used in sequencing, expression analysis, hybridization analysis, genetic analysis, RNA analysis or protein binding assays.
  • the use may be on an automated sequencing instrument.
  • the sequencing instrument may contain two lasers operating at different wavelengths.
  • kits comprise a plurality, particularly two, more particularly four, nucleotides labeled with a dye compound
  • the different nucleotides may be labeled with different dye compounds, or one may be dark, with no dye compounds.
  • the different nucleotides are labeled with different dye compounds it is a feature of the kits that said dye compounds are spectrally distinguishable fluorescent dyes.
  • the term “spectrally distinguishable fluorescent dyes” refers to fluorescent dyes that emit fluorescent energy at wavelengths that can be distinguished by fluorescent detection equipment (for example, a commercial capillary based DNA sequencing platform) when two or more such dyes are present in one sample.
  • the spectrally distinguishable fluorescent dyes can be excited at the same wavelength, such as, for example by the same laser in some embodiments.
  • two of the spectrally distinguishable fluorescent dyes can both be excited at one wavelength and the other two spectrally distinguishable dyes can both be excited at another wavelength in some embodiments.
  • Particular excitation wavelengths are about 460 nm.
  • At least one nucleotide may be labelled with a dye which excitable with two lasers with different wavelength.
  • a kit comprises a nucleotide labeled with a compound described herein and a second nucleotide labeled with a second dye wherein the dyes have a difference in absorbance maximum of at least 10 nm, particularly 20 nm to 50 nm. More particularly the two dye compounds have Stokes shifts of between 15-40 nm or between 20-40 nm. As used herein, the term “Stokes shift” is the distance between the peak absorption and peak emission wavelengths.
  • kit further comprises two other nucleotides labeled with fluorescent dyes wherein said dyes are excited by the lasers at about 440 nm to about 560 nm.
  • kits may contain nucleotides where the same base is labeled with two different compounds.
  • a first nucleotide may be labeled with a compound described herein.
  • a second nucleotide may be labeled with a spectrally distinct compound, for example a ‘red’ dye absorbing at greater than 600 nm.
  • a third nucleotide may be labeled as a mixture of the fluorescent dye compound described herein and the spectrally distinct compound, and the fourth nucleotide may be ‘dark’ and contain no label.
  • the nucleotides 1-4 may be labeled ‘green’, ‘red’, ‘red/green’, and dark.
  • nucleotides 1-4 can be labeled with a two dyes excited with a single laser, and thus the labeling of nucleotides 1-4 may be ‘green 1’, ‘green 2’ ‘green 1/green 2’, and dark.
  • kits may include a polymerase enzyme capable of catalyzing incorporation of the nucleotides into a polynucleotide.
  • Other components to be included in such kits may include buffers and the like.
  • the nucleotides labeled with the new fluorescent dyes described herein, and other any nucleotide components including mixtures of different nucleotides, may be provided in the kit in a concentrated form to be diluted prior to use. In such embodiments a suitable dilution buffer may also be included.
  • Nucleotides (or nucleosides) comprising a new fluorescent dye described herein may be used in any method of analysis which requires detection of a fluorescent label attached to a nucleotide or nucleoside, whether on its own or incorporated into or associated with a larger molecular structure or conjugate.
  • Some embodiments of the present application are directed to methods of sequencing including: (a) incorporating at least one labeled nucleotide as described herein into a polynucleotide; and (b) detecting the labeled nucleotide(s) incorporated into the polynucleotide by detecting the fluorescent signal from the new fluorescent dye attached to said modified nucleotide(s).
  • At least one labeled nucleotide is incorporated into a polynucleotide in the synthetic step by the action of a polymerase enzyme.
  • a polymerase enzyme e.g., a DNA sequence complementary to a nucleotide sequence.
  • other methods of incorporating labeled nucleotides to polynucleotides such as chemical oligonucleotide synthesis or ligation of labeled oligonucleotides to unlabeled oligonucleotides, are not excluded. Therefore, the term “incorporating” a nucleotide into a polynucleotide encompasses polynucleotide synthesis by chemical methods as well as enzymatic methods.
  • the detection step may be carried out whilst the polynucleotide strand into which the labeled nucleotides are incorporated is annealed to a template strand, or after a denaturation step in which the two strands are separated. Further steps, for example chemical or enzymatic reaction steps or purification steps, may be included between the synthetic step and the detection step.
  • the target strand incorporating the labeled nucleotide(s) may be isolated or purified and then processed further or used in a subsequent analysis.
  • target polynucleotides labeled with modified nucleotide(s) as described herein in a synthetic step may be subsequently used as labeled probes or primers.
  • the product of the synthetic step (a) may be subject to further reaction steps and, if desired, the product of these subsequent steps purified or isolated.
  • Suitable conditions for the synthetic step will be well known to those familiar with standard molecular biology techniques.
  • the synthetic step may be analogous to a standard primer extension reaction using nucleotide precursors, including modified nucleotides according to the present disclosure, to form an extended target strand complementary to the template strand in the presence of a suitable polymerase enzyme.
  • the synthetic step may itself form part of an amplification reaction producing a labeled double stranded amplification product comprised of annealed complementary strands derived from copying of the target and template polynucleotide strands.
  • Other exemplary “synthetic” steps include nick translation, strand displacement polymerization, random primed DNA labeling etc.
  • the polymerase enzyme used in the synthetic step must be capable of catalyzing the incorporation of modified nucleotides according to the present disclosure. Otherwise, the precise nature of the polymerase is not particularly limited but may depend upon the conditions of the synthetic reaction. By way of example, if the synthetic reaction is carried out using thermocycling then a thermostable polymerase is required, whereas this may not be essential for standard primer extension reactions. Suitable thermostable polymerases which are capable of incorporating the modified nucleotides according to the present disclosure include those described in WO 2005/024010 or WO 2006/120433.
  • polymerase enzymes need not necessarily be thermostable polymerases, therefore the choice of polymerase will depend on a number of factors such as reaction temperature, pH, strand-displacing activity and the like.
  • the modified nucleotides or nucleosides labeled with the new fluorescent dyes with longer Stokes shift according to the present application may be used in a method of nucleic acid sequencing, re-sequencing, whole genome sequencing, single nucleotide polymorphism scoring, any other application involving the detection of the modified nucleotide or nucleoside when incorporated into a polynucleotide, or any other application requiring the use of polynucleotides labeled with the modified nucleotides comprising fluorescent dyes according to the present application.
  • sequence-by-synthesis generally involves sequential addition of one or more nucleotides or oligonucleotides to a growing polynucleotide chain in the 5′ to 3′ direction using a polymerase or ligase in order to form an extended polynucleotide chain complementary to the template nucleic acid to be sequenced.
  • the identity of the base present in one or more of the added nucleotide(s) is determined in a detection or “imaging” step. The identity of the added base may be determined after each nucleotide incorporation step.
  • the sequence of the template may then be inferred using conventional Watson-Crick base-pairing rules.
  • the use of the modified nucleotides labeled with dyes according to the present disclosure for determination of the identity of a single base may be useful, for example, in the scoring of single nucleotide polymorphisms, and such single base extension reactions are within the scope of this application.
  • the sequence of a template polynucleotide is determined by detecting the incorporation of one or more nucleotides into a nascent strand complementary to the template polynucleotide to be sequenced through the detection of fluorescent label(s) attached to the incorporated nucleotide(s). Sequencing of the template polynucleotide is primed with a suitable primer (or prepared as a hairpin construct which will contain the primer as part of the hairpin), and the nascent chain is extended in a stepwise manner by addition of nucleotides to the 3′ end of the primer in a polymerase-catalyzed reaction.
  • each of the different nucleotide triphosphates may be labeled with a unique fluorophore and also comprises a blocking group at the 3′ position to prevent uncontrolled polymerization.
  • one of the four nucleotides may be unlabeled (dark).
  • the polymerase enzyme incorporates a nucleotide into the nascent chain complementary to the template polynucleotide, and the blocking group prevents further incorporation of nucleotides. Any unincorporated nucleotides are removed and the fluorescent signal from each incorporated nucleotide is “read” optically by suitable means, such as a charge-coupled device using laser excitation and suitable emission filters.
  • U.S. Pat. No. 5,302,509 discloses a method to sequence polynucleotides immobilized on a solid support. The method relies on the incorporation of fluorescently labeled, 3′-blocked nucleotides A, G, C and T into a growing strand complementary to the immobilized polynucleotide, in the presence of DNA polymerase.
  • the polymerase incorporates a base complementary to the target polynucleotide, but is prevented from further addition by the 3′-blocking group.
  • the label of the incorporated nucleotide can then be determined and the blocking group removed by chemical cleavage to allow further polymerization to occur.
  • the nucleic acid template to be sequenced in a sequencing-by-synthesis reaction may be any polynucleotide that it is desired to sequence.
  • the nucleic acid template for a sequencing reaction will typically comprise a double stranded region having a free 3′ hydroxyl group which serves as a primer or initiation point for the addition of further nucleotides in the sequencing reaction.
  • the region of the template to be sequenced will overhang this free 3′ hydroxyl group on the complementary strand.
  • the overhanging region of the template to be sequenced may be single stranded but can be double-stranded, provided that a “nick is present” on the strand complementary to the template strand to be sequenced to provide a free 3′ OH group for initiation of the sequencing reaction.
  • sequencing may proceed by strand displacement.
  • a primer bearing the free 3′ hydroxyl group may be added as a separate component (e.g. a short oligonucleotide) which hybridizes to a single-stranded region of the template to be sequenced.
  • the primer and the template strand to be sequenced may each form part of a partially self-complementary nucleic acid strand capable of forming an intra-molecular duplex, such as for example a hairpin loop structure.
  • Hairpin polynucleotides and methods by which they may be attached to solid supports are disclosed in PCT Publication Nos. WO 2001/057248 and WO 2005/047301. Nucleotides are added successively to the free 3′-hydroxyl group, resulting in synthesis of a polynucleotide chain in the 5′ to 3′ direction. The nature of the base which has been added may be determined, particularly but not necessarily after each nucleotide addition, thus providing sequence information for the nucleic acid template.
  • incorporation of a nucleotide into a nucleic acid strand (or polynucleotide) in this context refers to joining of the nucleotide to the free 3′ hydroxyl group of the nucleic acid strand via formation of a phosphodiester linkage with the 5′ phosphate group of the nucleotide.
  • the nucleic acid template to be sequenced may be DNA or RNA, or even a hybrid molecule comprised of deoxynucleotides and ribonucleotides.
  • the nucleic acid template may comprise naturally occurring and/or non-naturally occurring nucleotides and natural or non-natural backbone linkages, provided that these do not prevent copying of the template in the sequencing reaction.
  • the nucleic acid template to be sequenced may be attached to a solid support via any suitable linkage method known in the art, for example via covalent attachment.
  • template polynucleotides may be attached directly to a solid support (e.g. a silica-based support).
  • the surface of the solid support may be modified in some way so as to allow either direct covalent attachment of template polynucleotides, or to immobilize the template polynucleotides through a hydrogel or polyelectrolyte multilayer, which may itself be non-covalently attached to the solid support.
  • Arrays in which polynucleotides have been directly attached to silica-based supports are those for example disclosed in PCT Publication No. WO 2000/006770, wherein polynucleotides are immobilized on a glass support by reaction between a pendant epoxide group on the glass with an internal amino group on the polynucleotide.
  • PCT Publication No. WO2005/047301 discloses arrays of polynucleotides attached to a solid support, e.g. for use in the preparation of SMAs, by reaction of a sulfur-based nucleophile with the solid support.
  • a still further example of solid-supported template polynucleotides is where the template polynucleotides are attached to hydrogel supported upon silica-based or other solid supports.
  • Silica-based supports are typically used to support hydrogels and hydrogel arrays as described in PCT Publication Nos. WO 00/31148, WO 01/01143, WO02/12566, WO 03/014392, WO 00/53812 and U.S. Pat. No. 6,465,178.
  • a particular surface to which template polynucleotides may be immobilized is a polyacrylamide hydrogel.
  • Polyacrylamide hydrogels are described in the prior art, some of which is discussed above. Specific hydrogels that may be used in the present application include those described in WO 2005/065814 and U.S. Pub. No. 2014/0079923.
  • the hydrogel is PAZAM (poly(N-(5-azidoacetamidylpentyl) acrylamide-co-acrylamide)).
  • DNA template molecules can be attached to beads or microparticles for the purposes of sequencing; for example as described in U.S. Pat. No. 6,172,218. Further examples of the preparation of bead libraries where each bead contains different DNA sequences can be found in Margulies et al., Nature 437, 376-380 (2005), Shendure et al., Science. 309(5741): 1728-1732 (2005). Sequencing of arrays of such beads using nucleotides as described is within the scope of the present application.
  • the template(s) to be sequenced may form part of an “array” on a solid support, in which case the array may take any convenient form.
  • the method of the present disclosure is applicable to all types of “high density” arrays, including single-molecule arrays, clustered arrays and bead arrays.
  • Modified nucleotides labeled with dye compounds of the present application may be used for sequencing templates on essentially any type of array formed by immobilization of nucleic acid molecules on a solid support, and more particularly any type of high-density array.
  • the modified nucleotides labeled with the new fluorescent dyes described herein are particularly advantageous in the context of sequencing of clustered arrays.
  • distinct regions on the array comprise multiple polynucleotide template molecules.
  • the term “clustered array” refers to an array wherein distinct regions or sites on the array comprise multiple polynucleotide molecules that are not individually resolvable by optical means. Depending on how the array is formed each site on the array may comprise multiple copies of one individual polynucleotide molecule or even multiple copies of a small number of different polynucleotide molecules (e.g. multiple copies of two complementary nucleic acid strands).
  • Multi-polynucleotide or clustered arrays of nucleic acid molecules may be produced using techniques generally known in the art.
  • WO 98/44151 and WO 00/18957 both describe methods of amplification of nucleic acids wherein both the template and amplification products remain immobilized on a solid support in order to form arrays comprised of clusters or “colonies” of immobilized nucleic acid molecules.
  • the nucleic acid molecules present on the clustered arrays prepared according to these methods are suitable templates for sequencing using the modified nucleotides labeled with the new fluorescent dyes described herein.
  • the modified nucleotides labeled with dye compounds of the present application are also useful in sequencing of templates on single molecule arrays.
  • the term “single molecule array” or “SMA” as used herein refers to a population of polynucleotide molecules, distributed (or arrayed) over a solid support, wherein the spacing of any individual polynucleotide from all others of the population is such that it is possible to effect individual resolution of the polynucleotides.
  • the target nucleic acid molecules immobilized onto the surface of the solid support should thus be capable of being resolved by optical means. This means that, within the resolvable area of the particular imaging device used, there must be one or more distinct signals, each representing one polynucleotide.
  • each molecule is individually resolvable and detectable as a single molecule fluorescent point, and fluorescence from said single molecule fluorescent point also exhibits single step photo-bleaching.
  • the terms “individually resolved” and “individual resolution” are used herein to specify that, when visualized, it is possible to distinguish one molecule on the array from its neighboring molecules. Separation between individual molecules on the array will be determined, in part, by the particular technique used to resolve the individual molecules.
  • the general features of single molecule arrays will be understood by reference to PCT Publication Nos. WO 2000/006770 and WO 2001/057248.
  • one application of the modified nucleotides of the present disclosure is in sequencing-by-synthesis reactions, the utility of such labeled nucleotides is not limited to such methods. In fact, the nucleotides may be used advantageously in any sequencing methodology which requires detection of fluorescent labels attached to nucleotides incorporated into a polynucleotide.
  • the modified nucleotides labeled with dye compounds of the present application may be used in automated fluorescent sequencing protocols, particularly fluorescent dye-terminator cycle sequencing based on the chain termination sequencing method of Sanger and co-workers.
  • Such methods generally use enzymes and cycle sequencing to incorporate fluorescently labeled dideoxynucleotides in a primer extension sequencing reaction.
  • So called Sanger sequencing methods, and related protocols rely upon randomized chain termination with labeled dideoxynucleotides.
  • the present disclosure also encompasses modified nucleotides labeled with dye compounds as described herein which are dideoxynucleotides lacking hydroxyl groups at both of the 3′ and 2′ positions, such modified dideoxynucleotides being suitable for use in Sanger type sequencing methods and the like.
  • Table 2 summarizes the yield, characterization data, and spectral properties of the new coumarin fluorescent dyes disclosed in the examples.
  • the coumarin fluorescent dyes disclosed herein is coupled with appropriate amino-substituted nucleotide A-LN3-NH 2 or C-LN3-NH 2 after activation of carboxylic group:
  • the dye (10 ⁇ mol) is dissolved in dimethylformamide (1 mL) and then solvent is distilled off in vacuo. This procedure is repeated two more times.
  • the dried dye is dissolved in N,N-dimethylacetamide (DMA, 0.2 mL) in a 5 mL round-bottomed flask at room temperature.
  • DMA N,N-dimethylacetamide
  • N,N,N′,N′-Tetramethyl-O—(N-succinimidyl)uronium tetrafluoroborate (TSTU, 1.5 eq., 15 ⁇ mol, 4.5 mg) is added to the flask than N,N-diisopropylethylamine (DIPEA, 3 eq., 30 ⁇ mol, 3.8 mg, 5.2 ⁇ L) is added via micropipette to this solution. Reaction flask is sealed under nitrogen gas. After 15 minutes, the reaction progress is monitored by TLC (eluent H 2 O/Acetonitrile 1:9) and HPLC.
  • DIPEA N,N-diisopropylethylamine
  • N-LN3-NH 2 derivatives (20 mM, 1.5 eq, 15 ⁇ mol, 0.75 mL) is concentrated in vacuo then re-dissolved in water (20 ⁇ L).
  • Solution of the activated dye in DMA is transferred to the flask containing the solution of N-LN3-NH 2 .
  • More DIPEA (3 eq, 30 ⁇ mol, 3.8 mg, 5.2 ⁇ L) is added along with triethylamine (1 ⁇ L)
  • Progress of coupling is monitored hourly by TLC, HPLC and LCMS.
  • triethylamine bicarbonate buffer (TEAB, 0.05M approx., 3 mL) is added via pipette.
  • TEAB triethylamine bicarbonate buffer
  • Initial purification of the fully functionalized nucleotide is carried out by running the quenched reaction mixture through a DEAE-Sephadex® column (Sephadex poured into an empty 25 g Biotage cartridge, solvent system TEAB/MeCN). This removes most remaining dye.
  • Table 3 summarizes the structure and spectral properties of various nucleotides labelled with new coumarin dyes disclosed herein.
  • ffA-LN3-Dye refers to a fully functionalized A nucleotide with LN3 linker and labeled with a coumarin dye disclosed herein.
  • ffC-LN3-Dye refers to a fully functionalized C nucleotide with LN3 linker and labeled with a coumarin dye disclosed herein.
  • the R group in each of the structures refers to the coumarin dye moiety after conjugation.
  • nucleic acids can be sequenced utilizing methods and systems described in U.S. Patent Application Publication No. 2013/0079232, the disclosure of which is incorporated herein by reference in its entirety.
  • a nucleic acid can be sequenced by providing a first nucleotide type that is detected in a first channel, a second nucleotide type that is detected in a second channel, a third nucleotide type that is detected in both the first and the second channel and a fourth nucleotide type that lacks a label that is not, or minimally, detected in either channel.
  • the scatterplots were generated by RTA2.0.93 analysis of an experiment to compare the relative intensities of fully functionalized A nucleotide labeled with I-16, Dy510XL and ChromeoTM 494.
  • the comparisons were made in the same run (same flow cell and sequencing reagents) by rehybridizing the sequencing primer and performing a short (26 cycles) SBS run.
  • the scatterplots illustrated in FIG. 1 through FIG. 3 were at cycle 5 of each of the 26 cycle runs.
  • FIG. 1 illustrates the scatterplot of a fully functionalized nucleotide (ffN) mixture containing: A-I-16 (2 ⁇ M), C-NR440 (2 ⁇ M), dark G (2 ⁇ M) and T-NR550S0 (1 ⁇ M) in incorporation buffer with Pol812 (Blue exposure (Chan 1) 500 ms, Green exposure (Chan 2) 1000 ms; Scanned in Scanning mix).
  • ffN fully functionalized nucleotide
  • FIG. 2 illustrates the scatterplot of a ffN mixture containing: A-DY510XL (2 ⁇ M), C-NR440 (2 ⁇ M), darkG (2 ⁇ M) and T-NR550S0 (1 ⁇ M) in incorporation buffer with Pol812 (Blue exposure (Chan 1) 500 ms, Green exposure (Chan 2) 1000 ms; Scanned in SRE).
  • FIG. 3 illustrates the scatterplot of a ffN mixture containing: A-CH494 (2 ⁇ M), C-NR440 (2 ⁇ M), darkG (2 ⁇ M) and T-NR550S0 (1 ⁇ M) in incorporation buffer with Pol812 (Blue exposure (Chan 1) 500 ms, Green exposure (Chan 2) 1000 ms; Scanned in Scanning mix).
  • G nucleotide is unlabeled and shown as the lower left cloud (“dark G”).
  • the signal from the new coumarin dye I-16, DY510XL, and CH494 labeled “A” nucleotide is shown as the upper right cloud in FIGS. 1, 2, and 3 respectively.
  • the signal from the NR550S0 dye labeled “T” nucleotide is indicated by the upper left cloud, and NR440 dye labeled “C” nucleotide signal is indicated by the lower right cloud.
  • the X-axis shows the signal intensity for one channel and the Y-axis shows the signal intensity for the other channel.

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