M281175 八、新型說明： 【新型所屬之技術領域】 本新型係關於一種生物晶片，特別是由可使注入之反應試劑 能均勻覆蓋固著於底面之生物探針的中空容器所組成。 【先前技術】 近年來，由於微型化與微機電技術的快速發展，生物晶片已 成為用來研究基因功能及生命科學與現代醫療診斷發展的重要工 •具。再加上生物晶片具有體積輕巧、使用樣品（試劑）量少、反 應速度快、大量平行處理及可拋棄鱗優點，因此已廣泛地應用 纽物技術研究上，如：基因表現分析與治療、疾病與病毒檢測、 藥物筛選與暖以及個人化醫療等方面，而這些應_將會為整 個人類社會帶來革命性的衝擊。 傳統上，生物晶片1GG之設計多為於—載玻片⑽上固著生 物探針112，如第i圖所示。於使用時，操作者會將微量檢體滴 籲於載玻片U0上，再以蓋玻片120小心地覆蓋於上，最後再將整 ^片100 £入特製的反應盒内進行雜交反應。但是，採用此覆 盖盍玻片方式之生物晶片，於使用上报難控制檢體於晶片上的有 效體積量，並且於蓋玻片的使用操作上還必須要非常小心，避免 刮傷晶片上的探針。 而後’為了解決蓋玻片的使用不便以及反應體積量的控制不 易’因而發展出各式雜交反應盒，即將傳統之生 整合。其設計原理相當触，都是_預域切好酸面賴i M281175 材作為框架，雙面膠的上層膠用來黏著上蓋，下層膠則用來黏著 固著生物探針的載玻片。 參照如第2圖，於使用時，操作者先將框架230黏贴於固著 生物4木針212之載玻片210上，接著注入反應試劑，再將上蓋 站上另一種則是已將固著生物探針之載玻片⑽、框架33〇和 上蓋320依序黏貼在一起，操作者於使用時則藉由上蓋划之小 孔322注入反應試劑，然後再將小孔您覆蓋住，以免水分由小 φ孔322蒸發，如第3圖所示。 此類生物晶片的設計，確實解決了蓋玻片與載玻片之間操作 的不舰，然而操作者依然必須事切框雜貼於載玻片上，並 且於兀成反應之後，操作者須繼續進行晶片清洗與後續的偵測反 應’此時試劑的進出則造成相當不便。再加上，框架的厚度相當 薄’因此反應試劑注入後會完全和上下壁面翻，此時，由於表 =張力的作用’注人之試劑將完全靜止，因而反應的進行僅能靠 籲刀子的自然擴散來達成，難以借助外在的震動力量，協助反應的 速進行因此，對於習知之生物晶片實有改進之必要。 【新型内容】 5於以上的問題’本新型的主要目的在於提供—種生物晶 片藉以大體上解決先前技術所存在之問題。 本新型所揭露的生物晶片，可提伽m檢體進行生化反應。 本新型所揭露的生物晶片，可使注人之反應試劑均勻覆蓋底 面上固著之生物探針。 M281175 波動 瓜新受所揭露的生物晶Η ‘之汉應試劑於其内自由 晶 本新塑所揭露的生物晶片，可採用一體成型之方式製造。 因此，為達上述目的，本新型所揭露之生物晶片，包括：一 片載體.羯以固著複數個生物探針··一個以上反雜，娜於 生物探針設置於W載體上，其中，反應框内侧向外傾斜，’妙 成一傾斜面；以及—上蓋，用以覆蓋反應框，且與反應框之接觸 面密合。 其中’此傾斜面可為向外傾斜之一平面、向外傾斜之—弧面 或分段傾斜面。砂反雜之相可向賴斜，或者係大致 直於晶片載體。並且，於反應框之麵可具有一溝槽。 對庫，上盍可為一抛棄式覆蓋件；或者係由—個以上之側邊 =各個反雜之位置而設胁—蓋底上之結構，射反應框之 、、表物較㈣之雜略小·邊之高度小於反應框之高度。於 日士，’ =讀反應框之酬密合，且#反應框之糊具有一溝槽 ㈣應試射藉由毛細作用而導流人溝射，並填滿溝 明士 ^關t新型的特徵與實作，茲配合圖示作最佳實施例詳細說 的_ ^ 本發明的目的、構造、特徵、及其功能有進—步 ^鉍配合實施例詳細說明如下。） 【貫施方式】 以下舉出具體實施例以詳細說明本新型之内容，並以圖示作 M281175 為輔助說明。說明中提及之符號係參照圖式符號。 參照第4圖，於此，根據本新型一實施例之生物晶片，包括： -晶片載體410及-個以上反應框樣。其中，反應框42〇設置 於晶片載體410上，以形成開口成上之中空容器，且於容器内之 底面上固著複數個生物探針。換句話說，於晶片載體41〇上可固 著複數個生物探針化，而各個反應義各個生物探針群 412設置於晶片載體41〇上。於此，反應框42〇係以一體不可分 離之關係設置於晶片載體41〇上。 以下就生物晶片之單體加以詳細說明生物晶片之結構。 β ί…、苐5 6圖，係為本新型一實施例之生物晶片的單體 結構。於此，此單體係由一晶片載體和一反應框42〇組成； 其中’反應框420設置於晶片載體上，以形成開口成上 空容器。M281175 8. Description of the new type: [Technical field to which the new type belongs] This new type relates to a biochip, especially a hollow container that allows the injected reaction reagent to evenly cover the biological probe fixed on the bottom surface. [Previous technology] In recent years, due to the rapid development of miniaturization and micro-electromechanical technology, biochips have become important tools for studying gene functions and the development of life sciences and modern medical diagnostics. In addition, biochips have the advantages of light weight, small amount of samples (reagents), fast reaction speed, large number of parallel processing, and disposable scales. Therefore, they have been widely used in research on new technology, such as gene expression analysis and treatment, and disease. And virus detection, drug screening and warming, and personalized medicine, etc., and these should bring revolutionary impacts on the entire human society. Traditionally, the design of the biochip 1GG is mostly that the bioprobe 112 is fixed on the slide glass, as shown in Fig. I. During use, the operator will apply a small amount of sample to the slide U0, and then carefully cover it with a cover slip 120, and finally put the entire slice into a special reaction box for hybridization reaction. However, in the case of the biochip using the cover glass method, it is difficult to control the effective volume of the specimen on the wafer in the report of the use, and the use of the cover glass must be very careful to avoid scratching the probe on the wafer. needle. Then, in order to solve the inconvenience of the use of coverslips and the difficult control of the reaction volume, various hybridization reaction cassettes have been developed, which will integrate the traditional life. The design principle is quite touching, both of which are pre-cut cut acid surface and M281175 material as the frame. The upper layer of double-sided adhesive is used to adhere to the upper cover, and the lower layer of adhesive is used to adhere to the glass slide to which the biological probe is fixed. Referring to FIG. 2, in use, the operator first adheres the frame 230 to the glass slide 210 holding the biological needle 212, and then injects the reaction reagent, and then attaches the cover to the other station. The slide glass 生物, the frame 33, and the upper cover 320 which are attached to the bioprobe are sequentially adhered together. When using, the operator injects the reaction reagent through the small hole 322 marked on the upper cover, and then covers the small hole to avoid it. Water evaporates from the small φ holes 322, as shown in FIG. The design of this type of biochip really solves the problem of operation between the cover glass and the slide. However, the operator must still stick the frame to the slide, and after the reaction, the operator must continue Perform wafer cleaning and subsequent detection reactions. At this time, the in and out of reagents cause considerable inconvenience. In addition, the thickness of the frame is quite thin, so after the reaction reagent is injected, it will completely turn over the upper and lower walls. At this time, due to the effect of table = tension, the injected reagent will be completely stationary, so the reaction can only be performed by calling the knife. It is achieved by natural diffusion, and it is difficult to use the external vibration force to assist the speed of the reaction. Therefore, it is necessary to improve the conventional biochip. [New content] 5 problems above 'The main purpose of this new model is to provide a kind of biological wafer to substantially solve the problems existing in the prior art. The biochip disclosed by the novel model can be used for carrying out biochemical reactions on gamma specimens. The new type of biochip disclosed can uniformly cover the bioprobe fixed on the bottom surface with the injection reaction reagent. M281175 Fluctuation of the biological crystals disclosed by Guaxin ‘The Han Ying reagent is free to crystallize therein. The biochips disclosed by this new plastic can be manufactured by one-piece molding. Therefore, in order to achieve the above-mentioned purpose, the biochip disclosed in the present invention includes: a carrier. A plurality of bioprobes are fixed. One or more anti-hybrids are provided on the W carrier. Among them, the reaction The inside of the frame is tilted outwards, 'Miaocheng is an inclined surface; and-the upper cover is used to cover the reaction frame and is in close contact with the contact surface of the reaction frame. Among them, this inclined surface may be a plane inclined outward, an arc surface inclined outward, or a segmented inclined surface. The anti-sand phase may be oblique, or it may be approximately straight to the wafer carrier. Moreover, a groove may be provided on the surface of the reaction frame. For the library, the top can be a disposable cover; or it can be set by more than one side = each anti-miscellaneous position-the structure on the bottom of the cover, shooting the reaction frame, and the surface is more complicated Slightly smaller · The height of the side is smaller than the height of the reaction frame. In Ri Shi, '= the reward of reading the reaction frame is close, and the #reaction frame paste has a groove. The test shot guides people to gutter by capillary action and fills the groove. In conjunction with the implementation, the best embodiment is described in detail with the illustration. ^ The purpose, structure, features, and functions of the present invention are further advanced. The bismuth cooperation embodiment is described in detail below. ) [Performance Mode] The following are specific examples to explain the content of the new model in detail, and the illustration is used as an auxiliary description of M281175. The symbols mentioned in the description refer to the drawing symbols. Referring to FIG. 4, a biochip according to an embodiment of the present invention includes: a wafer carrier 410 and more than one reaction frame. Among them, the reaction frame 42 is disposed on the wafer carrier 410 to form an open hollow container, and a plurality of biological probes are fixed on the bottom surface of the container. In other words, a plurality of bioprobes can be fixed on the wafer carrier 41o, and each reaction and each bioprobe group 412 is provided on the wafer carrier 41o. Here, the reaction frame 42o is set on the wafer carrier 41o in an integral and inseparable relationship. The structure of the biochip will be described in detail below with respect to the monomer of the biochip. β ..., 苐 56 are diagrams of a single structure of a biochip according to an embodiment of the present invention. Here, the single system is composed of a wafer carrier and a reaction frame 42. The 'reaction frame 420 is disposed on the wafer carrier to form an opening into an overhead container.
晶片載體410之外緣可與反應框·之下緣外徑大致上相 同，如第5圖所示。然而為便於操作者拿取，晶片載體之外 緣亦可較反應框42G之下緣外歡，如第6騎示。並且， 出反應框420之下緣外徑的晶片載體彻上進行標示設計，例如， 缺角、凹槽、凸起或穿孔等，赠為使用者辨湖。 接著 抑 1曰曰片載體410之外緣較反應框420之下緣外徑大 之早體進行下舰構設計贿，然“賴與反雜之下緣外狎 大致上相同之單體亦可進行下列贿之結構設計變化。 - 參照第7圖，反應框420之頂侧與晶片載體之表面具有 M281175 一既定高度，此既定高度係依據欲注入之反應試劑的量而定，也 就疋，當注入反應試劑時，反應試劑之高度不高於此既定高度。 其中，此既定高度較佳範圍約為01〜0·5公分。此外，為使注入之 反應試劑能均勻覆蓋底面上固著之生物探針，因此於結構上，中 空容器之上_徑略大於下緣内徑，也狀，反應框42Q之内侧 係為向外傾斜之-傾斜面422，如第8、9圖所示。於此，此傾斜 面422内側與晶片載體410間之較佳夾角θ為約12〇度。The outer edge of the wafer carrier 410 may be substantially the same as the outer diameter of the reaction frame and lower edge, as shown in FIG. 5. However, for the convenience of the operator, the outer edge of the wafer carrier may be more pleasant than the lower edge of the reaction frame 42G, as shown in the sixth ride. In addition, the wafer carrier that has an outer diameter outside the lower edge of the reaction frame 420 is completely labeled, such as a notch, a groove, a protrusion, or a perforation, which is presented to the user to identify the lake. Next, the outer edge of the carrier 410 is larger than the lower edge of the reaction frame 420. The outer body has a lower carrier design bribe. The following structural design changes are made.-Referring to Figure 7, the top side of the reaction frame 420 and the surface of the wafer carrier have a predetermined height of M281175. This predetermined height depends on the amount of the reaction reagent to be injected, that is, When the reaction reagent is injected, the height of the reaction reagent is not higher than the predetermined height. Among them, the predetermined height is preferably in the range of about 01 to 0.5 cm. In addition, in order to inject the reaction reagent to uniformly cover the fixed surface on the bottom surface The biological probe is structurally, the diameter above the hollow container is slightly larger than the inside diameter of the lower edge, and it is also shaped. The inside of the reaction frame 42Q is an inclined surface-422, as shown in Figs. Here, a preferred angle θ between the inside of the inclined surface 422 and the wafer carrier 410 is about 120 degrees.
其中，反應框420可為向外傾斜之對稱結構，也就是反應框 420之外側亦為向外傾斜’如第8圖所示。另外，反應框伽亦 可為底寬頂窄之結構’也就是反應框樣之外侧大致與晶片載體 垂直而關（即，傾斜面422)向外傾斜，如第9圖所示。 於此，傾斜面422可為向外傾斜之一平面（如第8、9圖所 不），亦可為向外傾斜之-弧面（如㈣、n圖所示）。於此，此 弧面可為向反應框物外側凹之弧面（如第ig圖所示），亦或為 向反應框伽内側凹之弧面（如第u圖所示）。除此之外，此傾 斜面422還可為一分段傾斜面，其中每一 、 與:片載體形成一夾角，且此些夾角依據每—段::置 冒加。也就是說，當傾斜面422為兩段 成—第，而下段Among them, the reaction frame 420 may be a symmetrical structure inclined outward, that is, the outer side of the reaction frame 420 is also inclined outward, as shown in FIG. 8. In addition, the reaction frame can also have a structure with a wide bottom and a narrow top, that is, the outside of the reaction frame is approximately perpendicular to the wafer carrier and closed (that is, the inclined surface 422) is inclined outward, as shown in FIG. Here, the inclined surface 422 may be a plane inclined outward (as shown in Figs. 8 and 9), or it may be an arc surface inclined outward (as shown in Figs. ㈣ and n). Here, the arc surface may be a curved surface concave toward the outside of the reaction frame (as shown in Fig. Ig), or a curved surface concave toward the inside of the reaction frame (as shown in Fig. U). In addition, the inclined surface 422 can also be a segmented inclined surface, where each of the angles and the sheet carrier form an included angle, and these included angles are added according to each section of the :: set. In other words, when the inclined surface 422 is composed of two sections—the first section and the lower section
Ln 纽第—夾角會較第二夹角大，如第u 第一夹角之較佳角度為約12。度，而第二夾角之The angle of Ln Newty—the angle will be larger than the second angle. For example, the preferred angle of the first angle of u is about 12. Degrees, and the second angle
車乂佳角度為約100度。 人月I M281175 一 "炅均勻覆蓋底面上固著之生物彳 可將反應框420之交角面作圓“ 有〈生物I針， 钻… 角處理（如第13圖所示)，亦可。 針對反應框420内侧之交角面作圓角處理（如第Μ圖所示）/、 ^外，竭賴之下側可為_侧，但不影響上側 之、、々構设計，也就是，下侧内凹4 可上侧仍為平面之設計，此 % ’面上之轉本_之生物晶因表面張力作用導The car angle is about 100 degrees. Human month I M281175-"Uniformly cover the fixed organisms on the bottom surface." The corners of the reaction frame 420 can be rounded. "<Bio I needle, drill ... corner treatment (as shown in Figure 13), can also be. Fillet the corner surfaces of the inside of the reaction frame 420 (as shown in Figure M) /, ^, except that the lower side can be the _ side, but it does not affect the design of the upper side, the structure, that is, The lower side is concave 4 and the upper side is still a flat design.
，難以拿取之航發生，如第15、16圖卿。而擺置於平面上時， 曰曰片載體之下側可以四周邊緣與平面接觸（如第15圖所示）， 以可僅以相對之二週邊緣與平面接觸（如第％圖所示）。 此外糾第Π、18圖，於反應框42〇頂側可設計一溝槽 ^田反應才匡420與一上盘蓋合時，反應框420之Τ'頁側會與上蓋 山口以使療發之反應試劑可藉由表面張力而填滿溝槽424，進 而使内部呈現氣密狀態。 另外，根據本新型之生物晶片更包括：一上蓋5〇〇。此上蓋 _ 5〇〇可為-抱棄式覆蓋件，例如··黏著片（adhesivestrip)等，以 於試劑反應期間覆蓋反應框，並與反應框物之接觸面密合， 如苐19圖所示。 此外，上蓋500亦可為一蓋體盤，此蓋體盤係由一個以上之 侧邊520對應各個絲框之位置而設置於一蓋底51〇上，如第2〇 圖所不。於此，側邊520可以一體不可分離之關係設置於蓋底51〇 上0 以下就蓋體盤之單體詳細說明。 M281175 “、、第21 ® ’於圖中顯示一蓋體盤之單體結構，於此，蓋 體盤係由-蓋底510和_側邊52〇所組成—開口向下之中空容 器。並且侧邊52G可以—體不可分離之關係設置於蓋底510上。 並且，側邊520之内徑較反應框42〇之上緣外徑略大，使上蓋能 輕易蓋合；而側邊520之高度較反雜420之高度略小，以確保 上蓋能與反應框420之接觸面密合，如第22圖所示之組合狀態。 於此，盖底510之外緣可與側邊52〇之下緣外徑大致相同， 如第22圖所示，而’為便於操作者拿取，蓋底510大小亦可較 側邊520之下緣外徑大，如第23圖所示。 此外’反應框420和側邊520可為圍成大致上矩形；亦可為 如第24、25圖所顯示之大致上為圓形之反應框42〇和側邊別分 別格配大致上為矩形之晶片載體彻和蓋底51();或者係如第％、 27圖所顯示之大致上為圓形之反應框42〇和側邊52〇分別搭配大 致上為圓狀晶倾和蓋底训；t然亦可配合實際需求 而設計成其他各種幾何形狀。 多個反應框420之生物晶片，其反應框42〇可藉由串聯方式 排列（如第4圖所示）’亦可以矩陣方式排列，如帛28圖所示。 此外，多個反應框之生物晶片可為反應框42〇相連之方式，如第 29圖所示。並且於晶片載體可設計—個以上之分離槽414， 可藉由此分離槽414剝離不使用之晶片載體物，換句話說，當 使用多個反應框420之生物晶片時，可藉由分離槽414輕易地折 斷晶片載體410,以分離不使用之晶片載體41〇，如帛3〇圖所示。 M281175 於此，其上盍則搭配具有多個側邊52〇，以配蓋各個反應框 420 ’如第31、32圖所示。 丽述之各個實施例可藉由擠壓射出、真空成型等一體成型式 之程序製造。並且可使用塑膠材料製造成型。 藉由根據本新型一實施例之生斗勿晶片進行生化反應，可同時 進行多組賴，亦可分開進行檢_。此外，其係為反應盒與生 物晶片整合，生物晶片，並齡上蓋之配合，操作者完成反應實 驗後’可接續在此晶片載體與反應框所形成之中空容器内進行清 洗與後續的化學反應操作，錢其絲ϋ之搭配使用。另外，由 於垂直側面設計容易發生反應試齡生物^上的體積分佈非常 不均勻，於試劑中間部分會有嚴重下陷之現象，進而造成反應效 率不均的現象；因此，於此中空容器係為傾斜側面之設計，並將 側面交接線作圓角處理，以使反應試劑能在最少量的情況下均句 覆蓋晶片底面的生物探針，進而反應結果也將較為平均且效率較 回。再者，根據本新型一實施例之生物晶片於反應框的頂端設計 有毛細導水防漏流道（即，溝槽），於搭配上蓋進行高溫雜交實驗 時，蒸氣於上蓋凝結水珠，水珠將經由毛細現象導入毛細導水防 漏流道，而形成氣密，防止蒸氣持續向外蒸散，以維持試劑的體 積與濃度。最後，若將注人之反應試劑的高度低於反應框的内壁 時，可配合震盪1§的使用以使試劑於中空容器内可自由波動，因 而試劑具有較佳的擾動能量，進而提昇擴散速率，增進反應速率。 傳、、先上使用蓋玻片或岔閉式雜交反應盒進行反應時，其反應時門 12 M281175 其反應時間可 :為彳日守，然而於此藉由液面可擾動的設計 縮短到兩小時内。 定本Γί柄伽前述之較佳實施_如上，然_用以限 習相像技藝者，在不脫離本新型之精神和範圍 内田可作㈣之更触卿’目此本新叙專娜護範圍須視 本說明書崎之帽專纖騎料者鱗。 、 【圖式簡單說明】 第1圖係為說明先前技術之生物晶片之俯視圖； 第2圖係為說明先前技術之生物晶片與其反應盒之俯視圖； 第3圖係為說明另一先前技術之生物晶片與其反應盒之俯視 第4圖係為說明根據本新型第一實施例之生物晶片的俯視 圖； 第5圖係為說明根據本新型第二實施例之生物晶片單體的俯 視圖； 第6圖係為說明根據本新型第三實施例之生物晶片單體的俯 視圖； 第7圖係為說明根據本新型第三實施例之生物晶片單體的截 面圖； 第8圖係為第7圖中之反應框之第一實施例的放大圖； 第9圖係為第7圖中之反應框之第二實施例的放大圖； 第10圖係為第7圖中之反應框之第三實施例的放大圖； 13 M281175 第11圖係為第7圖中之反應框之第四實施例的放大圖； 第12圖係為第7圖中之反應框之第五實施例的放大圖； 第13圖係為說明根據本新型第四實施例之生物晶片單體的 俯視圖， 第Μ圖係為說明根據本新型第五實施例之生物晶片單體的 俯視圖； 第15圖係為說明根據本新型第六實施例之生物晶片單體的 截面圖； 第16圖係為說明根據本新型第七實施例之生物晶片單體的 截面圖； 第17圖係為說明根據本新型第八實施例之生物晶片單體的 截面圖； ’ 第18圖係為第17圖中之反應框的放大圖； 第19圖係鱗17圖中之反應框覆蓋根據本新型第一實施例 之上蓋的截面圖； =囷係為5兒明根據本新型第二實施例之上蓋的俯視圖； 第21圖係為說明根據本新型第三實施例之上蓋單體的截面 圖； 第22圖係為第21圖之上蓋覆蓋反應框時的截面圖； •第23圖係為說明根據本新型第四實施例之上蓋單體的截 圖； 叫 第24圖係為說明根據本新型第九實施例之生物晶片單體之 14 M281175 俯視圖； 本新型第四實施例之上蓋單體的俯視 本新型第十實施例之生物晶片單體之 本新型第五實施例之上蓋單體的俯視 第28圖係為賴根據本新型料-實施例之生物晶片之俯 9 第29圖料綱根據掏型第十二實施狀生物晶片之俯 第3〇圖料綱根據本新鮮十三實施例之生物晶片 視圖；It ’s difficult to take the voyage, such as Figures 15 and 16. When placed on a flat surface, the lower side of the carrier can be in contact with the plane around the edge (as shown in Figure 15), so that it can be in contact with the plane with only two opposite edges (as shown in Figure%). . In addition, as shown in Figures Π and 18, a groove can be designed on the top side of the reaction frame 42. When the field reaction cover 420 is combined with a top plate cover, the T 'page side of the reaction frame 420 will be connected with the top cover for the treatment. The reaction reagent can fill the groove 424 by surface tension, thereby making the interior airtight. In addition, the biochip according to the present invention further includes: a cover 500. This cover_500 may be a disposable cover, such as an adhesive strip, to cover the reaction frame during the reagent reaction and to closely contact the contact surface of the reaction frame, as shown in Figure 19 Show. In addition, the upper cover 500 may also be a cover plate, and the cover plate is provided on a cover bottom 51 by one or more side edges 520 corresponding to the positions of the respective wire frames, as shown in FIG. 20. Here, the side edge 520 can be integrally and inseparably arranged on the cover bottom 51 °. The following describes the details of the cover plate unit. M281175 ", the 21st" shows the unitary structure of a lid plate in the figure. Here, the lid plate is composed of-the bottom 510 and the side 52-the hollow container with an opening downward. The side 52G can be set on the cover bottom 510 in a non-separable relationship. In addition, the inner diameter of the side 520 is slightly larger than the outer diameter of the upper edge of the reaction frame 42, so that the upper cover can be easily closed; The height is slightly smaller than the height of the anti-aliasing 420 to ensure that the upper cover can closely contact the contact surface of the reaction frame 420, as shown in the combined state shown in Figure 22. Here, the outer edge of the cover bottom 510 can be connected to the side 52. The outer diameter of the lower edge is approximately the same, as shown in FIG. 22, and 'for the convenience of the operator, the size of the cover bottom 510 can also be larger than the outer diameter of the lower edge of the side 520, as shown in FIG. 23. In addition, the response The frame 420 and the side 520 may be formed into a generally rectangular shape; or, as shown in Figs. 24 and 25, the substantially circular reaction frame 42 and the side are respectively provided with a substantially rectangular wafer carrier. Thor and cover bottom 51 (); or the substantially circular reaction frame 42 and side 52 as shown in Fig. 27 and Fig. 27, respectively. The above is a round crystal tilt and bottom cover training; of course, it can also be designed into other various geometric shapes according to actual needs. For a biochip with multiple reaction frames 420, its reaction frame 42 can be arranged in series (such as the fourth (Shown in the figure) 'can also be arranged in a matrix, as shown in Figure 28. In addition, the biochips of multiple reaction frames can be connected in a manner that the reaction frames are 42 °, as shown in Figure 29. And the wafer carrier can be designed — More than one separation groove 414, by which the unused wafer carrier can be peeled off, in other words, when using a plurality of biological wafers of the reaction frame 420, the wafer carrier can be easily broken by the separation groove 414 410, to separate the unused wafer carrier 41, as shown in Figure 30. M281175 Here, the upper part is equipped with a plurality of side edges 52, to cover each reaction frame 420 ', such as sections 31 and 32. As shown in the figure. The various embodiments of Lishu can be manufactured by an integral molding process such as extrusion injection and vacuum molding. And they can be molded using plastic materials. Reaction can be simultaneous Multiple sets of tests can be performed, and the inspection can be performed separately. In addition, it is the integration of the reaction box and the biochip, and the combination of the biochip and the top cover. After the operator completes the reaction experiment, he can continue to the wafer carrier and the reaction frame. The formed hollow container is used for cleaning and subsequent chemical reaction operations, and is used in combination with Qian Qisi. In addition, because the vertical side design is prone to reactions, the volume distribution on the test-aged organisms is very uneven, and it will be severe in the middle part of the reagent. The phenomenon of sagging results in uneven reaction efficiency; therefore, the hollow container is designed with an inclined side, and the side cross-connections are rounded so that the reaction reagents can be covered evenly in the smallest amount. The bioprobe on the bottom surface of the wafer will have a more even reaction result and a higher efficiency. Furthermore, the biochip according to an embodiment of the present invention is designed with a capillary water-conducting leak-proof flow channel (ie, a groove) at the top of the reaction frame. When a high-temperature hybridization experiment is performed with the cover, steam condenses water drops and water drops on the cover. The capillary phenomenon is introduced into the capillary water-conducting leak-proof flow channel to form an airtightness, and the steam is prevented from continuously evaporating outward to maintain the volume and concentration of the reagent. Finally, if the height of the injected reaction reagent is lower than the inner wall of the reaction frame, it can be used in conjunction with shaking 1§ to make the reagent freely fluctuate in the hollow container, so the reagent has a better perturbation energy, thereby increasing the diffusion rate And increase the reaction rate. When using a cover glass or bifurcated hybridization reaction box to perform the reaction, the reaction time is 12 M281175. The reaction time can be as follows: However, the design of the liquid level can be reduced to two hours. Inside. The above-mentioned preferred implementation of the fixed version Γί handle _ as above, but _ is used to limit the practice of similar skills, and without departing from the spirit and scope of this new model, Tian Tian can make a closer contact with each other. Look at the Saki Hat Special Fiber Rider Scales in this manual. [Brief description of the drawings] Figure 1 is a plan view illustrating a biochip of the prior art; Figure 2 is a plan view illustrating a biochip and a reaction box of the prior art; Figure 3 is a description of another prior art biochip Top view of a wafer and its reaction box. FIG. 4 is a top view illustrating a biochip according to the first embodiment of the present invention. FIG. 5 is a top view illustrating a biochip unit according to the second embodiment of the present invention. FIG. 6 is a plan view FIG. 7 is a plan view illustrating a biochip unit according to a third embodiment of the present invention; FIG. 7 is a cross-sectional view illustrating a biochip unit according to a third embodiment of the present invention; FIG. 8 is a reaction in FIG. 7 An enlarged view of the first embodiment of the frame; FIG. 9 is an enlarged view of the second embodiment of the reaction frame in FIG. 7; FIG. 10 is an enlarged view of the third embodiment of the reaction frame in FIG. 13 M281175 Figure 11 is an enlarged view of the fourth embodiment of the reaction frame in Figure 7; Figure 12 is an enlarged view of the fifth embodiment of the reaction frame in Figure 7; Figure 13 is To explain the fourth embodiment of the present invention, FIG. 15 is a plan view illustrating a biochip unit according to a fifth embodiment of the present invention; FIG. 15 is a cross-sectional view illustrating a biochip unit according to a sixth embodiment of the present invention; FIG. 16 is a cross-sectional view illustrating a biochip unit according to a seventh embodiment of the present invention; FIG. 17 is a cross-sectional view illustrating a biochip unit according to an eighth embodiment of the present invention; The enlarged view of the reaction frame in FIG. 17; FIG. 19 is a cross-sectional view of the reaction frame in FIG. 17 covering the upper cover according to the first embodiment of the present invention; Top view of the top cover; FIG. 21 is a cross-sectional view illustrating a single cover according to a third embodiment of the present invention; FIG. 22 is a cross-sectional view when the top cover covers a reaction frame according to FIG. 21; A screenshot illustrating a cover unit according to the fourth embodiment of the present invention; called FIG. 24 is a top view of a M281175 illustrating a biochip unit according to the ninth embodiment of the present invention; Looking down at the new The top view of the fifth embodiment of the fifth embodiment of the biochip monomer of the tenth embodiment. The top view of FIG. 28 is the top view of the biochip according to the novel material-example. Twelfth embodiment of the biochip top view Figure 30 Outline of the biochip according to the fresh thirteenth embodiment;
第25圖係為說明根據 圖， 第26圖係為說明根據 俯視圖； 第27圖係為說明根據 圖； 第31圖係為說明根據本新型第十四實施例之生物晶片與第 貝施例之上盍蓋合時的截面圖；以及 第32圖係為說明根據本新型第十五實施例之生物晶片與第 七貫施例之上蓋蓋合時的截面圖 【主要元件符號說明】 100 ..........................生物晶片 110 ..........................載玻片 112 ..........................生物探針 120 ..........................蓋玻片 M281175 200 ..........................生物晶片 210 ..........................載玻片 212 ........................................生物探針 220................................上蓋 230 ..........................框架 300 ..........................生物晶片 310 ..........................載玻片 320 ..........................上蓋Fig. 25 is an illustration according to the figure, Fig. 26 is an illustration according to the top view; Fig. 27 is an illustration according to the figure; and Fig. 31 is an illustration of the biochip and the first embodiment according to the fourteenth embodiment of the present invention. A cross-sectional view of the top cover when closing; and FIG. 32 is a cross-sectional view illustrating the cover of the biochip according to the fifteenth embodiment of the present invention and the top cover of the seventh embodiment. [Key component symbol description] 100 .. ........................ Biochip 110 ............. ... Slide 112 ............... Bioprobe 120 ............... ............. Cover slip M281175 200 ............... Biochip 210 ... ............. Slide 212 ............. ....... Bioprobe 220 ................... ... Top cover 230 ............... Frame 300 ... ......... Biochip 310 ............... Slide 320 ... ........ cover
322 小孔 330 ..........................框架 410 ..........................晶片載體 4102 ..........................内凹 412 ..........................生物探針 414 ..........................分離槽 420 ..........................反應框 4222 ..........................上段 4224 ..........................下段 422 ..........................傾斜面 424 ................................溝槽 500 .......................…上蓋 510 ..........................蓋底 520 ..........................侧邊 16 M281175322 small hole 330 ............... frame 410 ... ........ wafer carrier 4102 ............... recessed 412 .......... ... Bioprobe 414 ........... Separation slot 420. ............ Response box 4222 ............ .... upper paragraph 4224 ..... lower paragraph 422 ... ..... inclined surface 424 ........ groove 500 .. ................. Top cover 510 ............... Cover bottom 520 ........... side 16 M281175