CN216082766U

CN216082766U - Sperm analysis slide

Info

Publication number: CN216082766U
Application number: CN202122472948.5U
Authority: CN
Inventors: 杜仲秋; 甘泉; 李�浩; 梁波
Original assignee: Suzhou Basecare Medical Device Co ltd
Current assignee: Suzhou Basecare Medical Device Co ltd
Priority date: 2021-10-14
Filing date: 2021-10-14
Publication date: 2022-03-18
Anticipated expiration: 2031-10-14

Abstract

The utility model relates to the technical field of medical inspection, and particularly discloses a sperm analysis slide. The glass slide comprises a glass slide, wherein a first analysis area and a second analysis area are arranged on the glass slide, a mark point is arranged between the first analysis area and the second analysis area, an edge layer surrounding the second analysis area is arranged at the periphery of the second analysis area, the edge layer is provided with a first inlet facing the mark point and a first exhaust port deviating from the mark point, the first cover glass is glued with the first analysis area through a first adhesive, and the second cover glass is glued with the edge layer through a second adhesive; the first cover glass, the first adhesive and the first analysis area enclose a first cavity, and the height of the first cavity is between 5 and 7 micrometers; and the second cover glass, the second adhesive, the edge layer and the second analysis area enclose a second cavity, and the height of the second cavity is between 10 and 15 micrometers. The structure provides two analysis cavities with different heights for the sperm sample, and facilitates the detection of different indexes of the sperm sample.

Description

Sperm analysis slide

Technical Field

The utility model relates to the technical field of medical inspection, in particular to a sperm analysis slide.

Background

In the current society, the ratio of infertile couples of the right age is rising year by year, but limited by shortage of medical resources and influence of traditional thinking, only less than 10% of patients actually receive regular treatment; more patients are regretted for life because the treatment is not timely and the result misses the best opportunity. The treatment process involves the quality detection and analysis of sperm, and the carrier for analyzing sperm samples is a sperm analysis slide.

Sperm analysis slides currently in use are of a split configuration, including slides and coverslips. The cover glass is easy to slide and damage during operation, the detection specimen is easy to overflow, and the operation is inconvenient. Meanwhile, the detection indexes of the existing sperm analysis glass are too few, and the existing sperm analysis glass can only be used for detecting the activity and the concentration of the sperm and cannot detect the sperm morphology indexes.

SUMMERY OF THE UTILITY MODEL

The utility model aims to provide a sperm analysis slide, which aims to solve the problems of inconvenient slide operation and too few detection indexes.

In order to achieve the purpose, the utility model adopts the following technical scheme:

a sperm analysis slide comprising a slide, a first coverslip, and a second coverslip; the glass slide is provided with a first analysis area and a second analysis area, a mark point is arranged between the first analysis area and the second analysis area, the peripheral edge of the second analysis area is provided with an edge layer surrounding the second analysis area, the edge layer is provided with a first inlet facing the mark point and a first exhaust port deviating from the mark point, the first cover glass is glued with the first analysis area through a first adhesive, and the second cover glass is glued with the edge layer through a second adhesive; the first cover glass, the first adhesive and the first analysis area enclose a first cavity, and the height of the first cavity in the direction perpendicular to the glass slide is between 5 micrometers and 7 micrometers; the second cover glass, the second adhesive, the edge layer and the second analysis region enclose a second cavity, and the height of the second cavity in the direction perpendicular to the slide is between 10 micrometers and 15 micrometers.

The first adhesive located in the first analysis area is cured to form an adhesive layer, the adhesive layer is arranged at the periphery of the first analysis area, and the adhesive layer is provided with a second inlet facing the mark point and a second exhaust port facing away from the mark point.

Further, the size of the second inlet port is larger than the size of the second exhaust port.

Preferably, an information area is further arranged on the glass slide and used for storing sample information.

Further, the information area, the first analysis area and the second analysis area are sequentially arranged in parallel.

Preferably, the first inlet port is larger in size than the first exhaust port.

Preferably, the thickness of the second cover glass is 0.4 mm.

Preferably, the first cover glass has a length and a width of 20 mm and a thickness of 0.18 mm.

Preferably, the edge layer is formed by curing the ink.

Preferably, the first adhesive and the second adhesive are both UV adhesives.

The utility model has the beneficial effects that:

through the structural design of the sperm analysis slide, sperm samples at the marking points can respectively infiltrate into the first cavity and the second cavity under the action of the cavity pressure of the first cavity and the second cavity. The first inlet port enables the sperm sample to enter the first cavity, and the arrangement of the first exhaust port facilitates the discharge of air in the second cavity, so that the efficiency of the sperm sample permeating into the second cavity is improved, and the effect of the sperm sample permeating into the second cavity is guaranteed. Meanwhile, the arrangement of the edge layer also ensures that the height of the second cavity in the direction vertical to the glass slide can be adjusted within a limited range. The height of the first cavity in the direction vertical to the glass slide is limited to be between 5 and 7 micrometers, so that the sperms in the first cavity can be distributed in a single layer, the sperms can not be overlapped when an operator observes the sperms in the first cavity in the direction vertical to the glass slide, and the detection of the operator on the sperm morphology indexes can be smoothly completed through the improvement; the height of the second cavity in the direction perpendicular to the glass slide is limited to 10-15 microns, so that the sperm sample can move freely in the second cavity, and the accurate result of the detection of the sperm motility and concentration index by an operator is ensured. The structure optimization enables the detection of three indexes of the vitality, the concentration and the form of the sperm sample on one glass slide, thereby saving a large amount of cost such as manpower, material resources and time. So that the operator can work efficiently.

Drawings

FIG. 1 is a top view of a sperm analysis slide provided in accordance with an embodiment of the present invention;

FIG. 2 is an exploded view of a sperm cell analysis slide provided in accordance with an embodiment of the present invention.

In the figure:

100. a glass slide; 110. an edge layer; 121. a first exhaust port; 122. a first access port; 130. marking points; 140. an information area; 200. a first cover glass; 300. and a second cover glass.

Detailed Description

In order to make the technical problems solved, technical solutions adopted and technical effects achieved by the present invention clearer, the technical solutions of the embodiments of the present invention will be described in further detail below with reference to the accompanying drawings, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

In the description of the present invention, unless expressly stated or limited otherwise, the terms "connected," "connected," and "fixed" are to be construed broadly, e.g., as meaning permanently connected, removably connected, or integral to one another; can be mechanically or electrically connected; either directly or indirectly through intervening media, either internally or in any other relationship. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.

In the present invention, unless otherwise expressly stated or limited, "above" or "below" a first feature means that the first and second features are in direct contact, or that the first and second features are not in direct contact but are in contact with each other via another feature therebetween. Also, the first feature being "on," "above" and "over" the second feature includes the first feature being directly on and obliquely above the second feature, or merely indicating that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature includes the first feature being directly under and obliquely below the second feature, or simply meaning that the first feature is at a lesser elevation than the second feature.

The technical scheme of the utility model is further explained by the specific implementation mode in combination with the attached drawings.

As shown in fig. 1 and 2, the present embodiment provides a sperm analysis slide including a slide 100, a first cover slip 200, and a second cover slip 300; a first analysis area and a second analysis area are arranged on the glass slide 100, a mark point 130 is arranged between the first analysis area and the second analysis area, an edge layer 110 surrounding the second analysis area is arranged at the periphery of the second analysis area, the edge layer 110 is provided with a first inlet 122 facing the mark point 130 and a first exhaust port 121 deviating from the mark point 130, the first cover glass 200 is glued with the first analysis area through a first adhesive, and the second cover glass 300 is glued with the edge layer 110 through a second adhesive; the first cover glass 200, the first adhesive and the first analysis region enclose a first cavity, and the height of the first cavity in the direction perpendicular to the slide 100 is between 5 micrometers and 7 micrometers; the second coverslip 300, second adhesive, border layer 110 and second analysis area enclose a second cavity having a height in a direction perpendicular to the slide 100 of between 10 and 15 micrometers.

Through the structural design of the sperm analysis slide, the sperm sample at the marker 130 can infiltrate into the first chamber and the second chamber, respectively, under the pressure of the chambers of the first chamber and the second chamber. First inlet port 122 makes the sperm sample can enter into first chamber, and the setting up of first exhaust port 121 has made things convenient for the discharge of air in the second chamber to improve the efficiency that the sperm sample permeates the second chamber, guaranteed the effect that the sperm sample permeates the second chamber. At the same time, the provision of the edge layer 110 also ensures that the height of the second cavity in the direction perpendicular to the slide 100 can be adjusted within a defined range. The height of the first cavity in the direction perpendicular to the glass slide 100 is limited to be between 5 micrometers and 7 micrometers, so that the sperms in the first cavity can be distributed in a single layer, the sperms can not be overlapped when an operator observes the sperm sample in the first cavity in the direction perpendicular to the glass slide 100, and the detection of the operator on the sperm morphology index can be smoothly completed through the improvement; by limiting the height of the second cavity in the direction perpendicular to the slide 100 to be between 10 and 15 micrometers, the sperm sample can move freely in the second cavity, thereby ensuring that the operator can obtain an accurate result in the detection of the sperm motility and concentration index. The above structure optimization enables detection of three indexes of sperm sample's motility, concentration and morphology on one slide 100 at the same time, thereby saving a large amount of costs of manpower, material resources, time, etc. So that the operator can work efficiently.

The edge layer 110 consists of two L-shaped structures, the lateral parts of which are arranged on the side of the second analysis area remote from the marker point 130. In other embodiments of this embodiment, the edge layer 110 is composed of other shapes and structures, which are not listed here, and all can achieve the purpose of designing the second cavity.

Specifically, the size of the first intake port 122 is larger than the size of the first exhaust port 121. The above-mentioned contents ensure the infiltration efficiency of the sperm sample, and simultaneously avoid the occurrence of the condition that the sperm sample in the second chamber largely flows out from the first exhaust port 121.

Preferably, the marker 130 is depicted on the bottom end of the slide 100, and the placement of the marker 130 improves the efficiency of the operator in loading the sperm sample into the sperm analysis slide, ensuring that the sperm sample fills the first and second chambers. In other embodiments of this embodiment, the marking of the mark point 130 can be accomplished by attaching a label and processing a groove, and the specific manner is common knowledge in the art and will not be described herein.

In this embodiment, the first adhesive located in the first analysis area is cured to form an adhesive layer, the adhesive layer is disposed at the periphery of the first analysis area, and the adhesive layer is provided with a second inlet opening facing the mark point 130 and a second outlet opening facing away from the mark point 130. Above-mentioned setting makes the gluing layer when the first analysis area of bonding and first coverslip 200, can also play the effect that surrounds first chamber, has avoided the sperm sample overflow who is located first intracavity to flow out first chamber. The second inlet port makes the sperm sample can enter into first chamber, and the setting up of second gas vent has made things convenient for the discharge of air in the first chamber to improve the efficiency of sperm sample infiltration first chamber, guaranteed the effect of sperm sample infiltration first chamber.

Specifically, the size of the second inlet port is larger than the size of the second exhaust port. The sperm sample infiltration efficiency is guaranteed, and meanwhile the phenomenon that the sperm sample in the first cavity flows out from the second exhaust port in a large quantity is avoided.

Preferably, the slide 100 is further provided with an information area 140, and the information area 140 is used for storing sample information. The arrangement of the information area 140 avoids the condition of information loss of the sperm sample, and improves the accuracy of sample processing.

Specifically, the sample information is recorded on a label, and the label is attached to the information area 140. In other embodiments of this embodiment, the sample information can be recorded on the slide 100 by directly writing on the information area 140 and recording by processing grooves, and the specific manner is common knowledge in the art and will not be described herein.

Further, the information area 140, the first analysis area and the second analysis area are sequentially arranged in parallel. The arrangement simplifies the structure of the slide 100, reduces the space occupied by the slide 100, and facilitates batch processing of sperm analysis slides by an operator.

In the present embodiment, the thickness of the second cover glass 300 is 0.4 mm; the first cover glass 200 was 20 mm long and wide and 0.18 mm thick.

Preferably, the edge layer 110 is formed by curing ink. The ink has the characteristics of low cost, easiness in curing and difficulty in dissolving, can effectively avoid the adverse effect of the edge layer 110 on the sperm sample in the second cavity in the subsequent treatment process, and ensures the accuracy of the subsequent detection operation.

In this embodiment, the first adhesive and the second adhesive are both UV adhesives. The UV (Ultraviolet Rays) glue is an Ultraviolet ray curing glue, and has the advantages of high curing speed, good bonding effect, high bonding strength and the like. The requirement that the distance between the first cover glass 200 and the first analysis area is limited to be between 5 and 7 micrometers can be met by using the UV adhesive; the matching of the UV glue and the edge layer 110 can meet the requirement that the distance between the second cover glass 300 and the second analysis area is limited to 10-15 micrometers, the improvement can effectively avoid the situation that the distance between the cover glass and the glass slide 100 is difficult to adjust, and the error of the chamber height is reduced.

It should be understood that the above-described embodiments of the present invention are merely examples for clearly illustrating the present invention, and are not intended to limit the embodiments of the present invention. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. And are neither required nor exhaustive of all embodiments. Any modification, equivalent replacement, and improvement made within the spirit and principle of the present invention should be included in the protection scope of the claims of the present invention.

Claims

1. A sperm analysis slide, comprising:

a first cover glass (200) and a second cover glass (300);

the glass slide (100) is provided with a first analysis area and a second analysis area, a mark point (130) is arranged between the first analysis area and the second analysis area, an edge layer (110) surrounding the second analysis area is arranged at the periphery of the second analysis area, the edge layer (110) is provided with a first inlet (122) facing the mark point (130) and a first exhaust port (121) deviating from the mark point (130), the first cover glass (200) is glued with the first analysis area through a first adhesive, and the second cover glass (300) is glued with the edge layer (110) through a second adhesive;

the first cover glass (200), the first adhesive and the first analysis region enclose a first cavity, the height of the first cavity in a direction perpendicular to the slide (100) is between 5 micrometers and 7 micrometers; the second cover glass (300), the second adhesive, the edge layer (110), and the second analysis region enclose a second cavity having a height in a direction perpendicular to the slide (100) of between 10 and 15 micrometers.

2. The sperm analysis slide of claim 1, wherein the first adhesive in the first analysis zone is cured to form an adhesive layer disposed about the periphery of the first analysis zone, the adhesive layer defining a second inlet opening facing the indicia (130) and a second outlet opening facing away from the indicia (130).

3. A sperm analysis slide as described in claim 2, wherein the second inlet port is sized larger than the second vent port.

4. A sperm analysis slide according to claim 1, wherein an information area (140) is further provided on the slide (100), said information area (140) being adapted to store sample information.

5. A sperm analysis slide as described in claim 4, wherein said information zone (140), said first analysis zone, and said second analysis zone are juxtaposed in sequence.

6. The sperm analysis slide of claim 1 wherein the first inlet port (122) is sized larger than the first outlet port (121).

7. The sperm analysis slide of claim 1, wherein the second coverslip (300) has a thickness of 0.4 mm.

8. The sperm analysis slide of claim 1, wherein the first coverslip (200) is 20 mm long and wide and 0.18 mm thick.

9. The sperm analysis slide of claim 1, wherein the edge layer (110) is formed by curing an ink.

10. The sperm analysis slide of any of claims 1-9, wherein the first adhesive and the second adhesive are both UV glue.