CN109307758A - A kind of white blood cell detection kit - Google Patents

A kind of white blood cell detection kit Download PDF

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Publication number
CN109307758A
CN109307758A CN201811383531.8A CN201811383531A CN109307758A CN 109307758 A CN109307758 A CN 109307758A CN 201811383531 A CN201811383531 A CN 201811383531A CN 109307758 A CN109307758 A CN 109307758A
Authority
CN
China
Prior art keywords
dropper
electrode
blood cell
film
white blood
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201811383531.8A
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Chinese (zh)
Inventor
郭志程
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
GUANGZHOU FEIKANG BIOTECHNOLOGY CO Ltd
Original Assignee
GUANGZHOU FEIKANG BIOTECHNOLOGY CO Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
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Application filed by GUANGZHOU FEIKANG BIOTECHNOLOGY CO Ltd filed Critical GUANGZHOU FEIKANG BIOTECHNOLOGY CO Ltd
Priority to CN201811383531.8A priority Critical patent/CN109307758A/en
Publication of CN109307758A publication Critical patent/CN109307758A/en
Pending legal-status Critical Current

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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483Physical analysis of biological material
    • G01N33/487Physical analysis of biological material of liquid biological material
    • G01N33/49Blood
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966Animal cells
    • G01N33/56972White blood cells

Abstract

The present invention provides a kind of white blood cell detection kits, detection card including the multiple independent packagings being placed in external packing box, multiple buffering liquid pipes to match with detection card quantity are placed on detection card side, the side of buffering liquid pipe is placed with multiple sampling heads, detection card includes Test paper, blotting paper and gets stuck, Test paper and absorbent paper layer stacked group are together in getting stuck, and then overall package is in aluminium foil bag;Buffering liquid pipe includes tube body, and for buffer in tube body, nozzle passes through sealing film sealing;Sampling head includes cylinder, and inner barrel is provided with dropper, and needle tubing is fixed on the bottom of cylinder by connecting column, and the needle tubing is oppositely arranged up and down with dropper, and needle tubing is separated with dropper, and is furnished with detachable cap at the top of dropper.Raw white cell detection kit body is small, easy to use, and operation application is easy, does not need medium-and-large-sized supplementary instrument or equipment, investment reduction cost;It is stored at room temperature, is not necessarily to later maintenance;Testing precision is excellent.

Description

A kind of white blood cell detection kit
Technical field
The present invention relates to kit technical fields, and in particular to a kind of white blood cell detection kit.
Background technique
White blood cell count(WBC) mainly has counting by naked eye and instrument counting method.
Counting by naked eye is instilled in celebrating number disk with dilution by hemodilution certain multiple and after destroying red blood cell, A certain range of leukocyte count is counted under microscope, can acquire the sum of various leucocytes in every liter of blood through converting.
Instrument counting method, the cellanalyzer at most used at present are called blood cell analyzer, Hematometer, hemocytometer Number instrument etc., is that hospital clinical examines very widely used one of instrument.And five sorting techniques of modern cellanalyzer Many uses technology identical with very advanced flow cytometer now, such as scatters optical detection technique, sheath Flow Technique, laser Technology etc., the detection of multichannel single can get nearly 20 parameters.
A variety of classification can be carried out according to different methods, are such as divided into semi-automatic cellanalyzer, complete by the degree of automation Automatic blood cell analyzer and blood cell analysis work station, blood cell analysis assembly line;It is divided into capacitive, resistance by testing principle Anti- type, laser class, photoelectric type, joint-detection type, dry-type centrifugal laminated devices and damage-free type;By the level point of instrument classification leucocyte RBC analysis instrument is knitted for two points of groups, three points of groups, five points of groups, five points of group+nets.
Although people's multiple types leukocyte differential count self-reacting device is come out one after another, not only to limit its general because expensive And and its result only serve screening effect, there is no so far an instrument can completely replace microscope blood film carry out leucocyte Sort check.Therefore, clinically still there are many mechanisms using traditional microscope classification.Blood is painted into film, is passed through After Wright staining, under microscope, is counted respectively one by one by white blood cell morphology feature, obtain ratio or the institute of various leucocytes Account for percentage.In conjunction with white blood cell count(WBC) as a result, the absolute value of various leucocytes in every liter of blood can be found out indirectly.Accurate leucocyte As a result, from sturdy blood cell morphology basis and superior in quality blood smear making and dyeing, this is also for differential counting The key of quality control.So visual Microscopical Method For Detection has certain experience and technical requirements to operator, while needing corresponding Operation experiments platform and equipment use very inconvenient.
Summary of the invention
In view of the deficiencies of the prior art, the invention proposes a kind of white blood cell detection kits, at low cost, easy to use, The quantity of blood middle leukocytes can fast and accurately be measured.
To realize above-mentioned technical proposal, the present invention provides a kind of white blood cell detection kits, including are placed on outer packing The detection card of multiple independent packagings in box, detection card include Test paper, blotting paper and get stuck, the Test paper and In blotting paper stacked combination is placed in and gets stuck, then for overall package in aluminium foil bag, the Test paper includes insulating substrate, institute It states and offers large aperture single hole or small-bore round on insulating substrate, electrode film, the electricity are also covered on the insulating substrate The surface cover of pole film is covered with protection cap film, and it is that printing material is constituted that the electrode film surface, which is printed with using conductive silver paste or carbon slurry, Measure the working electrode in circuit, to electrode and reference electrode;It is placed on detection card side and matches with detection card quantity Liquid pipe is buffered, the buffering liquid pipe includes tube body, and for buffer in tube body, nozzle passes through sealing film sealing;It is placed on buffering The sampling head to match on liquid pipe side and with buffering liquid pipe quantity, the sampling head includes cylinder, the middle and upper part of the cylinder It is provided with locating ring, the inner barrel is provided with dropper, and the water dropper of the dropper is designed as infundibulate dropper mouth;Needle tubing passes through Connecting column is fixed on the bottom of cylinder, and the needle tubing is oppositely arranged up and down with dropper, and needle tubing is separated with dropper, at the top of dropper Equipped with detachable cap.
Preferably, the Test paper is made by the following method,
S1: insulating substrate processes round and location hole according to design size;
S2: electrode film is pasted on substrate, and conductive silver paste or carbon is then used to starch as printing material, using stencil Printing constitutes the working electrode in measurement circuit, to electrode and reference electrode and dries, and drying temperature is 50-100 DEG C, drying time For 30-60min;
S3: stick on protection epiphragma in electrode film surface.
Preferably, it is that 0.02M is selected from by Na that the buffer, which is concentration,2HPO4、NaH2PO4It forms or by Na2HPO4、 KH2PO4Composition, pH7.0-7.4.
Preferably, the electrode film is PES film or NC film.
Preferably, the protection epiphragma is polyethylene film.
Preferably, the insulating substrate is PVC board.
A kind of beneficial effect of white blood cell detection kit provided by the invention is:
1) raw white cell detection kit structure is simple, easy to use, specifically need to only open external packing box when test, then Some detection card is taken out, the hermetic bag of detection card is removed, inserts cards into the card slot of detecting instrument, then, inhaled with sampling head Take in the sealing film insertion tube of pierces buffer liquid pipe, gently get rid of after sample it is several lower so that sample outflow and buffering in sampling head needle tubing Liquid mixes, and then sheds sampling head cap, sample is added dropwise into detection card sample aperture, clock reaction 1 minute, pressing detection was pressed Button, instrument will test result output on a display screen.Compared to visual Microscopical Method For Detection, white blood cell detection kit is easy to operate, It can be completed in general environment, do not need particular test room;Compared to various Automated hematologic analyzers, this method only needs It is equipped with a portable instrumentation, price is insufficient the former 1 percent, and it is almost negligible, it is greatly saved cost of investment;
2) the detection card in raw white cell detection kit, buffering liquid pipe and sampling head are disposable product, can be kept away Exempt to reuse and lead to pollution of reporting to the leadship after accomplishing a task, and configured dosage using preceding corresponded to, without again in use process It is deployed, use is very convenient;
3) raw white cell detection kit body is small, easy to use;Operation application is easy, does not need medium-and-large-sized supplementary instrument Or equipment, investment reduction cost;It is stored at room temperature, is not necessarily to later maintenance;Testing precision is excellent.
Detailed description of the invention
Fig. 1 is schematic perspective view I of the invention.
Fig. 2 is schematic perspective view II of the invention.
Fig. 3 is the schematic perspective view of external packing box in the present invention.
Fig. 4 is the packaging structure schematic diagram that card is detected in the present invention.
Fig. 5 is the schematic perspective view that card is detected in the present invention.
Fig. 6 is the schematic diagram of internal structure that card is detected in the present invention.
Fig. 7 is the structural schematic diagram of Test paper in the present invention.
Fig. 8 is the structural schematic diagram that liquid pipe is buffered in the present invention.
Fig. 9 is the perspective view I of sampling head in the present invention.
Figure 10 is the perspective view II of sampling head in the present invention.
In figure: 1, external packing box;11, box body;12, upper cover;13, forward position is bent;14, notch;2, detection card;21, it seals Bag;22, Test paper;221, insulating substrate;2211, location hole;2212, round;222, electrode film;2221, electrode;223, it protects Protecting cover film;23, blotting paper;24, it gets stuck;25, well;3, liquid pipe is buffered;31, tube body;32, film is sealed;4, sampling head;41, Cap;42, locating ring;43, cylinder;44, connecting column;45, needle tubing;46, infundibulate dropper mouth;47, dropper.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete Whole description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.Ability Domain ordinary person every other embodiment obtained without making creative work, belongs to protection of the invention Range.
A kind of embodiment: white blood cell detection kit.
Referring to figs. 1 to shown in Figure 10, a kind of white blood cell detection kit, including external packing box 1, the external packing box 1 are wrapped Box body 11 is included, openable and closable upper cover 12 is provided at the top of the box body 11, the front of upper cover 12 is provided with bending forward position 13, described Bending forward position 13 is inserted into box body 11, and setting jagged 14 at the top of 11 front end face of box body, to facilitate bending forward position 13 Quick insertion box body 11 is opened out of box body 11.The detection card of 20 independent packagings is placed in the external packing box 1 2, detection card 2 includes Test paper 22 and blotting paper 23 and gets stuck 24, and the Test paper 22 and blotting paper 23 are laminated Be combined in and get stuck in 24, then for overall package in hermetic bag 21, the Test paper 22 includes insulating substrate 221, it is described absolutely Location hole 2211 is offered on edge substrate 221, is also provided with the small-bore round 2212 of 4 × 4 matrixes distribution, the insulating substrate Electrode film 222 is also covered on 221, the surface cover of the electrode film 222 is covered with protection cap film 223,222 surface of electrode film It is printed with and constitutes the working electrode in measurement circuit, to electrode and reference electrode (2221- electrode), 222 surface of electrode film printing electricity Pole material is that conductive silver paste or carbon are starched, and Test paper 22 is made by the following method: first by insulating substrate 221 according to setting Size is counted using laser mode processing round and location hole;Then electrode film 222 is pasted on insulating substrate 221, is then made It uses conductive silver paste or carbon to starch as printing material, is printed using stencil and constitute the working electrode in measurement circuit, to electrode and ginseng It than electrode and dries, drying temperature is 85 DEG C, drying time 50min;Finally stick on protection epiphragma on 222 surface of electrode film 223;Wherein insulating substrate 221 is PVC board, and electrode film 222 is PES film (polyethersulfone resin membrane), and protection epiphragma 223 is polyethylene Film needs to tear hermetic bag 21 in specific detection, then takes out detection card;The detection, which blocks, is placed with 20 on 2 sides A buffering liquid pipe 3 to match with detection 2 quantity of card, the buffering liquid pipe 3 includes tube body 31, and buffer is mounted in tube body 31, Buffer is the phosphate buffer solution of concentration 0.02M, pH7.2;The mouth of tube body 31 is sealed by sealing film 32;The buffer The side of pipe 3 is placed with 20 sampling heads 4 to match with buffering 3 quantity of liquid pipe, and the sampling head 4 includes cylinder 43, institute The middle and upper part for stating cylinder 43 is provided with locating ring 42, and the cylinder 43 is internally provided with dropper 47, and the water dropper of the dropper 47 is set It is calculated as infundibulate dropper mouth 46;Needle tubing 45 is fixed on the bottom of cylinder 43 by connecting column 44, the needle tubing 45 on dropper 47 Under be oppositely arranged, needle tubing 45 is separated with dropper 47, at the top of dropper 47 be furnished with detachable cap 41;Sampling head and buffer The needle tubing 45 of sampling head 4 is stretched in sample first when specifically used, quantitative sample is drawn under capillarity by pipe Then product puncture sealing film 32 with the needle tubing 45 of sampling head 4, sampling head is inserted into buffering liquid pipe, gently get rid of several lower so that sampling Sample outflow in scalp acupuncture pipe 45 is mixed with buffer, then sheds sampling head cap 41, sampling head 4 is swung to, squeeze tube body 31 ooze sample mixed liquor (into the well 25 of detection card 2) from infundibulate dropper mouth 46.
In the present embodiment, in specific test, external packing box 1 only need to be opened, some detection card 2 is then taken out, removes inspection The hermetic bag 21 of card 2 is surveyed, detection card is taken out, inserts cards into the card slot of detecting instrument, then, first by the needle tubing of sampling head 4 45 stretch in sample, and quantitative sample is drawn under capillarity, then puncture sealing film with 4 needle tubing 45 of sampling head 32, sampling head is inserted into buffering liquid pipe, gently gets rid of several lower so that the sample outflow in sampling head needle tubing 45 is mixed with buffer, so After shed sampling head cap 41, sampling head 4 is swung to, squeeze tube body 31 make sample mixed liquor from infundibulate dropper mouth 46 ooze to In the well 25 of detection card 2;Clock reaction 1 minute, pressing detection button, instrument will test result output on a display screen. The working electrode in measurement circuit is constituted, to electrode and reference using conductive silver paste or carbon slurry since 222 surface of electrode film is printed with Electrode, by the variation of measuring electrode impedance, is calculated using the standard curve in instrument and is obtained in the case where given voltage The quantity of blood middle leukocytes out.
It is easy to operate using raw white cell detection kit compared to visual Microscopical Method For Detection, it can be completed in general environment, Particular test room is not needed;Compared to various Automated hematologic analyzers, only needed using the operation of raw white cell detection kit Be equipped with a portable instrumentation, price is insufficient the former 1 percent, almost negligible, be greatly saved investment at This.
In addition, the detection card 2, buffering liquid pipe 3 and sampling head 4 in raw white cell detection kit are disposable product, It can lead to pollution of reporting to the leadship after accomplishing a task to avoid reuse, and configure dosage using preceding corresponded to, nothing in use process It need to be deployed again, use is very convenient.
Raw white cell detection kit body is small, easy to use;Operation application is easy, do not need medium-and-large-sized supplementary instrument or Equipment, investment reduction cost;It is stored at room temperature, is not necessarily to later maintenance;Testing precision is excellent.
The above is presently preferred embodiments of the present invention, but the present invention should not be limited to embodiment and attached drawing institute public affairs The content opened both falls within protection of the present invention so all do not depart from the lower equivalent or modification completed of spirit disclosed in this invention Range.

Claims (6)

1. a kind of white blood cell detection kit, characterized by comprising:
It is placed on the detection card of multiple independent packagings in external packing box, the detection card includes Test paper, blotting paper and card In shell, the Test paper and blotting paper stacked combination are placed in and get stuck, then in aluminium foil bag, the detection tries overall package Paper includes insulating substrate, and large aperture single hole or small-bore round are offered on the insulating substrate, is also pasted on the insulating substrate Be covered with electrode film, the surface cover of the electrode film is covered with protection cap film, the electrode film surface be printed with using conductive silver paste or Carbon slurry is that printing material constitutes the working electrode in measurement circuit, to electrode and reference electrode;
The buffering liquid pipe for being placed on detection card side and matching with detection card quantity, the buffering liquid pipe includes tube body, is delayed For fliud flushing in tube body, nozzle passes through sealing film sealing;
The sampling head for being placed on buffering liquid pipe side and matching with buffering liquid pipe quantity, the sampling head includes cylinder, institute The middle and upper part for stating cylinder is provided with locating ring, and the inner barrel is provided with dropper, and the water dropper of the dropper is designed as infundibulate Dropper mouth;Needle tubing is fixed on the bottom of cylinder by connecting column, and the needle tubing and dropper are oppositely arranged up and down, needle tubing and dropper it It is separated, it is furnished with detachable cap at the top of dropper.
2. white blood cell detection kit as described in claim 1, it is characterised in that: the Test paper is made by the following method ,
S1: insulating substrate processes round and location hole according to design size;
S2: electrode film is pasted on substrate, is then used conductive silver paste or carbon to starch as printing material, is printed using stencil It constitutes the working electrode in measurement circuit, to electrode and reference electrode and dry, drying temperature is 50-100 DEG C, and drying time is 30-60min;
S3: stick on protection epiphragma in electrode film surface.
3. white blood cell detection kit as claimed in claim 2, it is characterised in that: the buffer is that concentration is 0.02M choosing Free Na2HPO4、NaH2PO4It forms or by Na2HPO4、KH2PO4Composition, pH7.0-7.4.
4. white blood cell detection kit as claimed in claim 2, it is characterised in that: the electrode film is PES film or NC film.
5. white blood cell detection kit as claimed in claim 2, it is characterised in that: the protection epiphragma is polyethylene film.
6. white blood cell detection kit as described in claim 1, it is characterised in that: the insulating substrate is PVC board.
CN201811383531.8A 2018-11-20 2018-11-20 A kind of white blood cell detection kit Pending CN109307758A (en)

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Application Number Priority Date Filing Date Title
CN201811383531.8A CN109307758A (en) 2018-11-20 2018-11-20 A kind of white blood cell detection kit

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Application Number Priority Date Filing Date Title
CN201811383531.8A CN109307758A (en) 2018-11-20 2018-11-20 A kind of white blood cell detection kit

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Publication Number Publication Date
CN109307758A true CN109307758A (en) 2019-02-05

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112295620A (en) * 2020-10-12 2021-02-02 中国计量大学 Saliva draws crystallization micro-fluidic chip fast
CN113122426A (en) * 2021-03-08 2021-07-16 顾余 Biological cell sap test box capable of being detected at any time and conveniently
CN115379899A (en) * 2020-04-07 2022-11-22 瑞士斯保德精密诊断有限公司 Assay device

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Publication number Priority date Publication date Assignee Title
US20020048532A1 (en) * 2000-09-01 2002-04-25 Yueh-Hui Lin Disposable electrode for whole blood hemoglobin (HGB) and hematocrit (HCT) measurement, and preparation and application thereof
CN103424540A (en) * 2012-05-18 2013-12-04 嘉善加斯戴克医疗器械有限公司 Leukocyte classification kit and classification method thereof
CN105067685A (en) * 2015-07-23 2015-11-18 武汉大学 Blood multi-component detection test paper and preparation method thereof
WO2017154013A1 (en) * 2016-03-07 2017-09-14 Indian Institute Of Technology, Delhi An assay and kit for detection of endotoxin
CN107576788A (en) * 2017-11-03 2018-01-12 深圳市巴德生物科技有限公司 A kind of reference product for detecting leukocyte differentiation antigen and preparation method thereof
CN209086255U (en) * 2018-11-20 2019-07-09 广州菲康生物技术有限公司 A kind of white blood cell detection kit

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020048532A1 (en) * 2000-09-01 2002-04-25 Yueh-Hui Lin Disposable electrode for whole blood hemoglobin (HGB) and hematocrit (HCT) measurement, and preparation and application thereof
CN103424540A (en) * 2012-05-18 2013-12-04 嘉善加斯戴克医疗器械有限公司 Leukocyte classification kit and classification method thereof
CN105067685A (en) * 2015-07-23 2015-11-18 武汉大学 Blood multi-component detection test paper and preparation method thereof
WO2017154013A1 (en) * 2016-03-07 2017-09-14 Indian Institute Of Technology, Delhi An assay and kit for detection of endotoxin
CN107576788A (en) * 2017-11-03 2018-01-12 深圳市巴德生物科技有限公司 A kind of reference product for detecting leukocyte differentiation antigen and preparation method thereof
CN209086255U (en) * 2018-11-20 2019-07-09 广州菲康生物技术有限公司 A kind of white blood cell detection kit

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115379899A (en) * 2020-04-07 2022-11-22 瑞士斯保德精密诊断有限公司 Assay device
CN112295620A (en) * 2020-10-12 2021-02-02 中国计量大学 Saliva draws crystallization micro-fluidic chip fast
CN113122426A (en) * 2021-03-08 2021-07-16 顾余 Biological cell sap test box capable of being detected at any time and conveniently

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