TWI862531B - 突變型piggyBac轉位酶 - Google Patents

突變型piggyBac轉位酶 Download PDF

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Publication number
TWI862531B
TWI862531B TW108145098A TW108145098A TWI862531B TW I862531 B TWI862531 B TW I862531B TW 108145098 A TW108145098 A TW 108145098A TW 108145098 A TW108145098 A TW 108145098A TW I862531 B TWI862531 B TW I862531B
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seq
piggybac
translocase
nucleic acid
acid sequence
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TW108145098A
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Chinese (zh)
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TW202035687A (zh
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尼勒 Ｊ 阿格拉沃
克麗斯汀 Ｍ 達瑞斯
珍妮特 Ｌ 史蒂芬斯
宏帝諾 黎
塔拉凡 諾利亞 布藍可
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美商安進公司
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K40/00—Cellular immunotherapy
    • A61K40/10—Cellular immunotherapy characterised by the cell type used
    • A61K40/11—T-cells, e.g. tumour infiltrating lymphocytes [TIL] or regulatory T [Treg] cells; Lymphokine-activated killer [LAK] cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K40/00—Cellular immunotherapy
    • A61K40/30—Cellular immunotherapy characterised by the recombinant expression of specific molecules in the cells of the immune system
    • A61K40/32—T-cell receptors [TCR]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K40/00—Cellular immunotherapy
    • A61K40/40—Cellular immunotherapy characterised by antigens that are targeted or presented by cells of the immune system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
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    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70503—Immunoglobulin superfamily
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0681—Cells of the genital tract; Non-germinal cells from gonads
    • C12N5/0682—Cells of the female genital tract, e.g. endometrium; Non-germinal cells from ovaries, e.g. ovarian follicle cells
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    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
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    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
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    • C12N2800/00—Nucleic acids vectors
    • C12N2800/10—Plasmid DNA
    • C12N2800/106—Plasmid DNA for vertebrates
    • C12N2800/107—Plasmid DNA for vertebrates for mammalian
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    • C12N2800/00—Nucleic acids vectors
    • C12N2800/90—Vectors containing a transposable element

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  • Animal Behavior & Ethology (AREA)
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  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Mycology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Cell Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Reproductive Health (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Hematology (AREA)
  • Developmental Biology & Embryology (AREA)
TW108145098A 2018-12-10 2019-12-10 突變型piggyBac轉位酶 TWI862531B (zh)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201862777325P 2018-12-10 2018-12-10
US62/777,325 2018-12-10
US201962925516P 2019-10-24 2019-10-24
US62/925,516 2019-10-24

Publications (2)

Publication Number Publication Date
TW202035687A TW202035687A (zh) 2020-10-01
TWI862531B true TWI862531B (zh) 2024-11-21

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EP (1) EP3894549B1 (enExample)
JP (1) JP7441840B2 (enExample)
KR (1) KR102999228B1 (enExample)
CN (2) CN113195712B (enExample)
AU (2) AU2019398101B2 (enExample)
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US20230348561A1 (en) 2022-04-28 2023-11-02 Immatics US, Inc. Dominant negative tgfbeta receptor polypeptides, cd8 polypeptides, cells, compositions, and methods of using thereof
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CN121127601A (zh) * 2023-05-12 2025-12-12 隆萨生物制剂股份有限公司 使用转座酶进行混合瞬时转染以用于高产率产物生产
AU2024300966A1 (en) 2023-07-21 2026-01-08 Amgen Inc. Vectors incorporating a combination of promoters driving selectable marker expression
US20250134931A1 (en) 2023-11-01 2025-05-01 Immatics US, Inc. Membrane-bound il-15, cd8 polypeptides, cells, compositions, and methods of using thereof
CN120737218B (zh) * 2025-08-15 2026-01-06 云南彤宁生物制药技术有限公司 融合蛋白、调控系统及利用该系统构建转基因细胞的方法

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SG11202106049RA (en) 2021-07-29
AU2026201887A1 (en) 2026-04-02
CL2025004022A1 (es) 2026-04-10
CA3121268A1 (en) 2020-06-18
CL2023003339A1 (es) 2024-06-07
CL2021001488A1 (es) 2021-11-26
DK3894549T3 (da) 2024-10-14
SI3894549T1 (sl) 2024-10-30
BR112021011063A2 (pt) 2021-08-31
JP2022512332A (ja) 2022-02-03
MX2021006783A (es) 2021-07-15
ES2991895T3 (es) 2024-12-05
EP3894549A1 (en) 2021-10-20
PT3894549T (pt) 2024-10-16
KR20210101270A (ko) 2021-08-18
US20220064610A1 (en) 2022-03-03
CN113195712B (zh) 2024-09-13
IL283314A (en) 2021-07-29
FI3894549T3 (fi) 2024-10-23
JP7441840B2 (ja) 2024-03-01
LT3894549T (lt) 2024-09-25
KR102999228B1 (ko) 2026-07-31
AU2019398101A1 (en) 2021-06-10
HUE068630T2 (hu) 2025-01-28
AU2019398101B2 (en) 2025-12-18
EP3894549B1 (en) 2024-07-31
PL3894549T3 (pl) 2024-11-25
TW202035687A (zh) 2020-10-01
WO2020123327A1 (en) 2020-06-18
CN113195712A (zh) 2021-07-30
CN118995660A (zh) 2024-11-22
US12570966B2 (en) 2026-03-10

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