TWI757830B - Use of glycyrrhiza extract in preparation of medicament for treating glottic insufficiency with inhalation - Google Patents

Abstract

The use of Glycyrrhiza extract in preparation of medicine for treating glottis insufficiency with inhalation is provided. The use includes: providing a Glycyrrhiza extract, and laryngeal administrating the solution to a subject in need thereof. The advantages of using the Glycyrrhiza extract include reduced side effects compared to Western medical treatment, improving patient compliance and significantly improving hoarse symptom compared to the currently available treatment.

Description

Use of licorice extract for preparing medicine for treating vocal cord insufficiency

The present invention relates to a licorice extract and its use for preparing a medicine for treating insufficiency of vocal cords, in particular to a licorice extract that can be used by fumigation to treat glottis atresia caused by vocal cord paralysis or vocal cord atrophy Incomplete.

Vocal cord insufficiency is caused by incomplete closure of the vocal cords, including vocal cord mobility and mucosal lesions of the vocal cords. Vocal cord activity lesions include unilateral vocal cord paralysis caused by neuromuscular and joint dislocation, while vocal cord mucosal lesions include vocal cord defects, scarring, atrophy, and vocal cord grooves, both of which can cause the glottis to fail to close, collectively referred to as vocal cord insufficiency.

Unilateral vocal cord paralysis is generally caused by surgery, tumor, or stroke. Symptoms include hoarseness or accompanying dysphagia, which may lead to pneumonia and may be life-threatening. Current treatments for unilateral vocal cord paralysis include speech therapy, vocal cord injection, and Body fat or hyaluronic acid, and thyroid cartilage surgery, etc.

As for the atrophy of the vocal cords, it is because the congenital or acquired collagen of the vocal cords itself is reduced, resulting in insufficient volume of the vocal cords, so that it is not enough to maintain the closing force of the glottis, resulting in weakness in vocalization. Or hoarseness in severe cases. Acquired collagen reduction is generally caused by aging or overuse of the vocal cords. The treatment methods can also use speech therapy, vocal cord injection of autologous fat or hyaluronic acid, and thyroid cartilage surgery.

Insufficiency of the glottis has always been a challenge faced by every otolaryngologist. Among the above three treatment methods, speech therapy has different effects. Vocal cord injections require their own expenses or cannot last for a long time, and thyroplasty surgery requires surgery. Risks and complications.

Therefore, it is necessary to provide a medicament that reduces side effects and can indeed improve and/or treat glottic insufficiency and its use.

In view of the above problems, the present invention provides a licorice extract whose active ingredient is licorice, which is a component of pure traditional Chinese medicine. The licorice extract contains no western medicines and steroids. It is fragrant, sweet, and non-invasive. It is highly accepted by patients and the public, and the medicinal materials are simple and cheap.

According to the purpose of the present invention, there is provided a use of a licorice extract for preparing a medicine for treating vocal cord insufficiency, which comprises providing a licorice extract; and applying the licorice extract to a target by fumigation by oral or nasal inhalation the throat.

The licorice extract provided by the present invention is obtained by decocting and extracting an appropriate amount of licorice with an aqueous solution to obtain a licorice extract having the active ingredient extract of licorice.

S10~S40: Steps

Figure 1 is a schematic diagram of the preparation process of the licorice extract of the present invention; Figure 2 is the analysis chart of VHI-10 of patients with vocal cord atrophy treated with traditional Chinese medicine with licorice extract before and after treatment; Figure 3 is the VHI-10 of patients with vocal cord paralysis treated with traditional Chinese medicine with licorice extract before and after treatment 10. Analysis chart; Figure 4a is a schematic diagram of the vocal cords of a patient with vocal cord paralysis before treatment; Figure 4b is a schematic diagram of the vocal cords of a patient with vocal cord paralysis after treatment.

In order to make the above-mentioned purposes, technical features and benefits after practical implementation easier for those skilled in the art to understand, the following will be described in more detail with examples and drawings.

Licorice has the following Chinese medicinal properties: flat in nature, sweet in taste, and returns to the twelve meridians; {function cures mainly} invigorating the spleen and replenishing qi, clearing away heat and detoxifying, eliminating phlegm and relieving cough, relieving acute pain and reconciling various medicines; treating spleen and stomach weakness, fatigue Fatigue, palpitations, shortness of breath, cough and phlegm, epigastric pain, contracture and pain of limbs, carbuncle and sore, relieving drug toxicity, strong.

The present invention treats patients with insufficiency of glottis by means of fumigating licorice extract, improves the symptoms of hoarseness and achieves better therapeutic effect.

Glottic insufficiency includes unilateral vocal cord paralysis or vocal cord atrophy.

Referring to Figure 1, it is a schematic diagram of the preparation process of the licorice extract of the present invention.

In step S10, the raw material of licorice (Glycyrrhiza) is pre-treated to facilitate subsequent steps. The pretreatment can be any technique known to those with ordinary knowledge in the technical field. Pretreatment can include washing, soaking, squeezing, chopping, crushing, rolling, grinding, and any physical and/or chemical Sexual pretreatment. The licorice raw material may comprise licorice roots, rhizomes, or a combination thereof. In addition, commercially available licorice decoction pieces (decocting pieces) can also be used as the licorice raw material.

In step S20, the pretreated licorice raw material is extracted. The extraction step may be any technique known to those of ordinary skill in the art. The extraction step may include stirring, heating, or sonication, among others. The extraction solvent can be water or an organic solvent. Preferably, the extraction solvent can be water. Furthermore, the extraction is carried out continuously or at intervals. In one embodiment, the total extraction time may be 60 to 240 minutes; more preferably 90 to 120 minutes. In one embodiment, the extraction temperature may be 80 to 300 degrees; more preferably, 120 to 180 degrees. In one embodiment, the weight ratio of the licorice raw material to the extraction solvent may be 1:15 to 1:35; more preferably, 1:20 to 1:30. In one embodiment, the licorice extract is a solid product obtained by removing the extraction solvent from the licorice extract. In one embodiment, the extraction rate of the licorice raw material can be calculated by using the dry weight of the licorice extract after removing the extraction solvent, and the extraction rate (%)=the dry weight of the licorice extract after extraction/the weight of the licorice raw material before extraction×100%. In one embodiment, the extraction rate of the licorice extract of the licorice raw material may be 10% to 30%. In another embodiment, the extraction rate of the licorice extract of the licorice raw material may be 10% to 50%. In certain embodiments, 5 g of licorice raw material can yield at least 0.5 g, 1 g, 1.5 g, 2 g, 2.5 g of licorice extract.

In step S30, the licorice extract that has undergone the extraction step is purified to obtain a licorice extract (step S40). The purification step can be performed by any technique known to those of ordinary skill in the art. Purification steps may include precipitation, filtration, centrifugation, column chromatography, thin film chromatography, or preparative high performance liquid chromatography, and the like.

In a preparation example, the licorice decoction pieces are selected as the licorice raw material, and water is used as the extraction solvent; the detailed preparation steps are as follows: get 450g (about 120 money) licorice decoction pieces, add 11000c.c. of water for decocting, decocting 2 hours. Generally speaking, 450g licorice pieces will absorb about 2000c.c. After filtering licorice pieces, 9000c.c. licorice extract can be obtained. Then, 9000c.c. licorice extract was divided into 90 packs, each pack was 100c.c. licorice extract. The weight of the licorice extract contained in the licorice extract is calculated as follows: through rough extraction, that is, all the moisture of the licorice extract is removed by a vacuum concentrator (IKA, Germany) to obtain 100 c.c. The licorice extract weighs 0.5 to 1.5 g.

The licorice extract can be stored in various ways, for example: the licorice extract can be directly packaged and stored, the licorice extract can be concentrated to a specific storage concentration and then packaged and stored, or the licorice extract can be packaged in the form of powder or crystalline particles after the drying step. save.

The drying step may be any technique known to those of ordinary skill in the art. The drying step may include room temperature evaporation, heating, freeze drying, or concentration under reduced pressure, and the like. In addition, the licorice extract can be directly packaged in a sterile state at high temperature, and can also be stored in the above-mentioned various forms at room temperature, in a refrigerator, a moisture-proof box or an oven.

In one embodiment, the licorice extract encapsulated at high temperature can remain sterile for at least three months at room temperature or in a refrigerator.

In one embodiment, the powder or crystalline particle form can be placed in an environment with controlled environmental humidity to increase its storage time.

Preferably, the licorice extract of the present invention can be stored until taken out before administration, and configured in a form suitable for administration. Preferably, it is stored until it is taken out before being prepared as a drug for treating insufficiency of vocal cords, and is configured into a form suitable for preparation.

In one embodiment, a predetermined dose of licorice extract is administered to the nose of the target. The target can be mammals, such as humans, cows, sheep, pigs, dogs, cats, horses, donkeys, rabbits, and the like.

In one embodiment, the route of administering the medicament for the treatment of vocal cord insufficiency of the present invention is inhalation administration. In one embodiment, the use of the present invention is administered The route for preparing the medicament for the treatment of vocal cord insufficiency is mouth and nose inhalation.

In one embodiment, a commercially available fumigation machine that can generate atomization can be used, the licorice extract is warmed and then poured into the fumigation machine to generate a spray, and then the patient is asked to wear a mask and inhale it through the mouth or nose. Spray to the throat for the treatment of glottis insufficiency.

In one embodiment, the frequency of fumigation treatment is 1 to 3 times a week; more preferably, at least 3 to 5 times a week; more preferably, at least 1 to 3 times a day.

In one embodiment, when using licorice extract for fumigation treatment, each fumigation time is at least 5 to 30 minutes; more preferably, each fumigation time is at least 10 to 20 minutes.

The use of licorice extract: Using the Patient Voice Handicap Index-10 (VHI-10), the patients were asked to self-assess their language barriers. The higher the score, the more serious the voice problem. Generally speaking, the score level of voice disorder is as follows: 0-5 is normal, 6-10 is mild, 11-20 is moderate, and 21-40 is severe.

Referring to Figure 2, it is the difference of VHI-10 before and after treatment of 6 patients treated with Chinese medicine with licorice extract. The treatment time of patients receiving traditional Chinese medicine treatment with licorice extract varies from half a month to half a year, with an average treatment time of 3 to 4 months. The results showed that the mean value of VHI-10 before treatment was 22.0 points (standard deviation: 7.5), and the mean value of VHI-10 decreased to 11.8 points (standard deviation: 7.0, P=0.03) after treatment.

From the difference in VHI-10 scores before and after treatment shown in Figure 2 above, it can be seen that the patients treated with traditional Chinese medicine with the licorice extract in this case have significantly improved their voice, which means that the use of licorice extract does have a significant effect. therapeutic efficacy.

Referring to Figure 3, it is the difference in VHI-10 before and after treatment of 5 patients treated with traditional Chinese medicine with licorice extract. As mentioned above, the treatment time of the patients receiving treatment varies from half a month to half a year, with an average treatment time of 3 to 4 months. The mean of VHI-10 before treatment was 27.4 points (standard deviation: 7.2), and the mean VHI-10 decreased to 4.6 points (standard deviation: 5.5, P=0.04) after treatment, and the mean VHI-10 decreased by 22.8 (about 80%).

Referring to Figure 4a and Figure 4b, they are respectively a schematic diagram of the vocal cords before and after treatment with licorice extract and Chinese medicine for a patient with vocal cord paralysis using laryngeal scintigraphy. The treatment time with licorice extract and Chinese medicine is about 3 months. . Figure 4a shows the vocal cords before the treatment. It can be seen that the muscles on both sides of the vocal cords have atrophied. After the treatment with Chinese medicine with licorice extract, it can be seen from Figure 4b that the muscles on both sides of the vocal cords have recovered a lot. In addition, the patient's voice has also improved significantly, and the hoarseness has improved a lot. Therefore, the use of licorice extract in combination with traditional Chinese medicine does have obvious therapeutic effects.

Claims (5)

A use of a licorice extract for preparing a medicine for treating vocal cord insufficiency, comprising: administering a licorice (Glycyrrhiza) extract to the throat of a target, wherein the licorice extract is obtained by extracting an aqueous solution. The use according to claim 1, wherein the route of administering the drug is after the licorice extract is atomized, and then administered to the throat by means of oral or nasal breathing. The use of claim 1, wherein the atresia of the vocal cords is caused by vocal cord atrophy or vocal cord paralysis. The use according to claim 1, wherein every 100 c.c of the licorice extract contains 0.1 g to 10 g of the licorice extract. The use according to claim 1 further comprises a traditional Chinese medicine.

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