TWI725715B - Caulerpa microphysa extract and product containing extract thereof - Google Patents

Abstract

A Caulerpa microphysa extract is extracted from a Caulerpa microphysa algae. The Caulerpa microphysa extract has an anti-wrinkle ingredient, an anti-allergy ingredient and an anti-oxidation ingredient which include algal polysaccharides, polyphenols and flavonoids.

Description

Small leaf fern algae extract and its added products

The present invention relates to a Caulerpa microphysa extract and its added products; in particular, it relates to a Caulerpa microphysa extract with anti-wrinkle and anti-allergy functions and its addition product.

Regarding the conventional fern and algae extract, its extraction method and its use, for example, the invention patent of the Republic of China Patent Publication No. TW-I592161 "The extract of Pteridophyta and its flavonoid extraction method", which discloses a kind of Pteridophyllum Algae extract and its flavonoid extraction method. The extract of Pteridium aquilinum contains a polysaccharide and a flavonoid, and the polysaccharide and flavonoids are extracted from a Pteridium aquilinum, and the flavonoid has anti-inflammatory, antibacterial and antioxidant capabilities. ability.

Continuing, the aforementioned method for extracting flavonoids from Pteridium aquilinum of No. TW-I592161 includes: providing a Pteridium pteridinium body and adding it to a solution; and pulverizing the Pteridium pteridinium body in the solution to obtain a flavonoid. Long stem Pteridium algae solution; hot water extraction treatment of the long stem Pteridium algae solution to obtain a long stem Pteridium algae extract; filtration treatment of the long stem Pteridium algae extract to obtain a filtered long stem Pteridium algae Extraction: The filtered Pteridium aquilinum extract is subjected to separation treatment to obtain a flavonoid-containing extract.

As a matter of fact, the aforementioned extract of Pteridium aquilinum and its flavonoid extraction method No. TW-I592161 only reveals the extract of Pteridium aquilinum and its anti-inflammatory, antibacterial and antioxidant capabilities. Revealing the polysaccharide extract of Pteris sylvestris and the flavonoids extract of Psyllium sylvestris Things and so on.

Another conventional fern and algae extract, its extraction method and its use, for example: the invention patent of the Republic of China Patent Publication No. TW-I558408 "A sea grape extract with inhibiting allergy, its preparation method and application", which discloses one Sea grape extract with inhibiting allergy, its preparation method and its application. The extraction method of the sea grape extract includes: Step 1. Beat the sea grape in pure water, wherein the weight ratio of the sea grape to the pure water is 1 part of the sea grape: 1 to 20 parts of pure water, and mixed uniformly to obtain A sea grape syrup; Step 2. Continue to stir the sea grape syrup, and the stirring temperature is 20 to 100 ℃, and the stirring time is 30 to 120 minutes; Step 3. The sea grape syrup is filtered for the first time to obtain Get a sea grape extract.

In addition, the aforementioned sea grapes No. TW-I558408 is selected from fresh sea grapes, dried sea grapes or sea grape powder. The conditions of the first filtration are 5 microns and 1 μm, and after the first filtration, the sea grape extract can be further subjected to a second filtration, and the second filtration is a membrane filtration.

In fact, the aforementioned extract of Pteris sylvestris and its flavonoid extraction method No. TW-I558408 only reveals the extract of Pseus sylvestris and its anti-inflammatory, antibacterial, and antioxidant abilities, and only The disclosure includes polysaccharide extracts of Pteridium aquilinum and flavonoid extracts of Pteridium aquilinum, etc.

Another conventional fern and algae extract, its extraction method and its use, such as: "Sea grape extract extraction method and preparation of pharmaceutical composition for inhibiting the growth of prostate cancer tumor cells" invention patent of the Republic of China Patent Publication No. I484966 , Which reveals a method for extracting sea grape extract. After drying and pulverizing the sea grape, the crude extract is carried out with an alcohol solvent, and the crude sea grape extract is obtained after concentration under reduced pressure. Then the crude sea grape extract was dissolved in ethanol solution, and then mixed with n-hexane [hexane] and separated [partition] to obtain ethanol solution isolate. Next, dissolve the ethanol Water and ethyl acetate were added to the liquid separation product, separated and concentrated under reduced pressure to obtain sea grape ethyl acetate separation product. After the ethyl acetate isolate of sea grape is subjected to column chromatography and other steps, the natural extract of sea grape [Excaulerpa] is obtained. A pharmaceutical composition is further provided, which contains an effective amount of the natural extract of sea grape for the purpose of inhibiting the growth of prostate cancer tumor cells.

In fact, the aforementioned natural extract of sea grape and its extraction method No. I484966 only reveals how to extract the natural extract of sea grape and add it to the preparation of a pharmaceutical composition that inhibits the growth of prostate cancer tumor cells.

Another conventional fern-algae extract, its extraction method and its use, for example: Chinese Patent Publication No. CN-109833334 "Preparation method and application of grape fern-algae extract", which discloses a long-stemmed invention patent application Preparation method and application of grape fern algae extract. The preparation method of the grape Pteridium longiflorum extract comprises: dissolving a sample of the Pteridium longiflorum algae in an extraction solvent, followed by grinding and stirring, ultrasonication, filtration or centrifugation, and concentration to obtain the extract of the Pteridium longiflorum. liquid.

In addition, the extraction solvent of the aforementioned No. CN-109833334 is selected from water or an aqueous ethanol solution, and the volume concentration of the aqueous ethanol solution is 0.5% to 100%, and the combination of the Botrytis cinerea sample and the extraction solvent The ratio is 1g:0.1 to 100ml. The long-stem grape Pteridium algae extract has the functions of moisturizing and replenishing water, anti-inflammatory, promoting cell proliferation and promoting wound healing.

In fact, the aforementioned Botrytis cinerea extract and the preparation method of the aforementioned No. CN-109833334 only reveal how to extract the Botrytis cinerea extract to obtain the Botrytis cinerea extract, that is, to dissolve the Botrytis cinerea sample in the In the extraction solvent to obtain the Botrytis cinerea extract.

Another conventional fern and algae extract, its extraction method and its use, such as: Republic of China Patent Publication No. TW-M584745 "Sea grape extract repair film applied to radiation damage" new patent, which discloses one This is a sea grape extract repair film used for radiation damage. The sea grape extract repair film applied to radiation damage includes a dressing layer and an attachment layer.

In addition, one side of the dressing layer of No. TW-M584745 is in contact with the attachment layer, and the other side of the dressing layer is used to attach a skin tissue, and the attachment layer is used to support and shape the dressing layer. The dressing layer contains sea grape extract, sea grape polysaccharides, amino acids, vitamins and minerals, which are substances that can induce activation or promote tissue repair. The dressing layer contains blue-green algae extract phycobiliprotein, which has the effect of tissue repair.

In fact, the aforementioned sea grape extract repair film No. TW-M584745 only reveals the sea grape extract and its function of inducing activation or promoting tissue repair, and only reveals the phycobiliprotein containing blue-green algae extract And its function to achieve tissue repair.

Another conventional fern and algae extract, its extraction method and its use, such as: Republic of China Patent Publication No. TW-M486428 "The fine particle structure of sea grape extract for whitening and/or skin moisturizing", which discloses A fine particle structure of sea grape extract that whitens and promotes skin moisturization. The particle structure of the sea grape extract for whitening and moisturizing the skin includes an active core layer and a protective layer, and the active core layer is arranged in the protective layer.

In addition, the particle structure of the sea grape extract with whitening and promoting skin moisturization of the aforementioned No. TW-M486428 further includes a moisturizing layer, and the moisturizing layer is located between the active core layer and the protective layer, and the moisturizing layer Encapsulate the active core layer. The particle structure of the sea grape extract for whitening and moisturizing the skin further includes a moisture-proof layer, and the moisture-proof layer is coated outside the protective layer.

In fact, the particle structure of the aforementioned sea grape extract of No. TW-M486428 only reveals the inclusion of sea grape extract and its function of skin whitening or skin moisturizing, and only reveals that it contains the active core layer, protective layer, The particle structure of the moisture-retaining layer and the moisture-proof layer.

However, the aforementioned Republic of China Patent Publication No. TW-I592161, No. TW-I558408, No. I484966, Chinese Patent Publication No. CN-109833334, Republic of China Patent Publication No. TW-M584745 and No. TW-M486428 patents and patents There is still a need for further improvement of the fern-algae extract, its extraction method and its use in the application.

The aforementioned Republic of China Patent Publication No. TW-I592161, No. TW-I558408, No. I484966, China Patent Publication No. CN-109833334, Republic of China Patent Publication No. TW-M584745 and No. TW-M486428 patents and patent applications It is only a reference for the technical background of the present invention and an explanation of the current state of technology development, and it is not intended to limit the scope of the present invention.

In view of this, in order to meet the above technical problems and needs, the present invention provides a Pteridium vulgare extract and an additive product thereof, wherein the Pteridium vulgare extract is extracted from a body of Pteridium vulgare, and the Pteridium vulgare extract has An anti-wrinkle ingredient, an anti-allergic ingredient and an anti-oxidant ingredient, and the anti-wrinkle ingredient, anti-allergic ingredient and antioxidant ingredient include algae polysaccharides, polyphenols and flavonoids, and the small leaf fern algae extract can be used as an anti-wrinkle ingredient , Anti-allergic and anti-oxidant application of physiological activities, in order to expand the purpose of the application of conventional fern and algae extracts.

The main purpose of the present invention is to provide a small leaf fern algae extract and an added product thereof, wherein the small leaf fern algae extract is extracted from a small leaf fern algae body, and the small leaf fern algae extract has an anti-wrinkle ingredient and an anti-allergic ingredient And an anti-oxidant ingredient, and the anti-wrinkle ingredient, anti-allergic ingredient and antioxidant ingredient include algae polysaccharides, polyphenols and flavonoids, and the Pteridium vulgare extract can be used as anti-wrinkle, anti-allergic and anti-oxidant physiological activities In order to achieve the purpose or effect of enhancing the economic value of Pteridium vulgaris, the extract of Pteridium vulgare is not cytotoxic.

In order to achieve the above object, the leaflet of the preferred embodiment of the present invention The extraction methods of Pteridium algae extract include:

Providing at least one or several small leaf fern algae bodies, and the small leaf fern algae bodies include several stalks, several contracted upright stems, several globular twigs and several false roots;

Using a homogenizer in a body of water to evenly crush the phyllophyllum algae body to obtain several pieces of the phyllophyllia algae body;

Extracting the fragments of the small leaf fern algae in a hot water to obtain a small leaf fern algae extract;

Cooling the small leaf fern algae extract to obtain a cooled small leaf fern algae extract, and use a centrifuge to precipitate and remove impurities to obtain a supernatant small leaf fern algae extract; and

The supernatant Pteridium aquilinum body extract is freeze-dried to obtain a Pteridium aquilinum body extract powder, and the Pteridium aquilinum body extract powder contains algae polysaccharides, polyphenols and flavonoids.

In a preferred embodiment of the present invention, the Pteridophyte body is an artificially cultivated Pteridophyte body.

In the preferred embodiment of the present invention, the artificially cultivated small leaf fern algae adopts a water temperature of 23±3°C and a seawater salinity of 32±3ppt.

The temperature of the hot water in the preferred embodiment of the present invention is 120°C.

In order to achieve the above-mentioned purpose, the Pteridium parvum extract of the present invention includes:

A small leaf fern algae extract is extracted from a small leaf fern algae body, and the small leaf fern algae extract has an anti-wrinkle ingredient, an anti-allergic ingredient and an anti-oxidant ingredient, and the anti-wrinkle ingredient, anti-allergic ingredient and antioxidant ingredient Contains algae polysaccharides, polyphenols and flavonoids.

In a preferred embodiment of the present invention, the Pteridophyte body is selected from the Pteridophyte body of different growth periods.

In a preferred embodiment of the present invention, the Pteridium parvum body includes several stems, several constricted upright stems, several globular twigs and several false roots.

The Pteridium parvum extract of the preferred embodiment of the present invention does not have cytotoxicity.

In order to achieve the above-mentioned purpose, the added product of Pteridium parvum extract of the preferred embodiment of the present invention includes:

At least one cosmetic raw material, which has a first content; and

At least one Pteridium vulgare extract having a second content, and the Pteridium vulgare extract is extracted from a Pteridium vulgare, and the Pteridium vulgare extract is added to the cosmetic material to prepare a cosmetic composition Thing

Wherein, the Pteridium parvum extract has at least one anti-wrinkle ingredient, at least one anti-allergic ingredient and at least one antioxidant ingredient, and the anti-wrinkle ingredient, anti-allergic ingredient and antioxidant ingredient include algae polysaccharides, polyphenols and flavonoids.

In a preferred embodiment of the present invention, the cosmetic material is selected from a skin care product material, a color cosmetic product material or a perfume material to make a skin care product, a color cosmetic product or a perfume.

In a preferred embodiment of the present invention, the cosmetic raw material is selected from a powdered raw material, a paste raw material or a liquid raw material.

In order to achieve the above-mentioned purpose, the added product of Pteridium parvum extract of the preferred embodiment of the present invention includes:

At least one food material, which has a first content; and

At least one Pteridium vulgare extract having a second content, and the Pteridium vulgare extract is extracted from a Pteridium vulgare, and the Pteridium vulgare extract is added to the food material to prepare a food composition Thing

Wherein, the Pteridium parvum extract has at least one anti-wrinkle ingredient, at least one anti-allergic ingredient and at least one antioxidant ingredient, and the anti-wrinkle ingredient, anti-allergic ingredient and antioxidant ingredient include algae polysaccharides, polyphenols and flavonoids.

The food composition of the preferred embodiment of the present invention is a beverage, a food, a seasoning sauce, a seasoning powder, a health food or a health care Supplies.

In the preferred embodiment of the present invention, the Pteridium vulgare extract is a Pteridium vulgare extract essence, a Pteridium vulgare extract essential oil, a Pteridium vulgare extract essence tablet, a Pteridium vulgare extract granule, and a Pteridium aureum extract Essence powder or any combination.

1: Small leaf fern algae

10: storage carrier

10a: Mask

10b: Patch

10c: gauze body

10d: Bandage roll

10e: capsule

10f: Packing bag

10g: Distributed box

10h: OK stretch

100: processing equipment

2: Cosmetic raw materials

20: Food ingredients

3: Small leaf fern algae extract

a: cosmetic composition

b: Food composition

Figure 1: A schematic block diagram of the Pteridium parvum extract additive product of the first preferred embodiment of the present invention.

Figure 2: A schematic diagram of the structure of the Pteridium vulgare extract additive product and various storage carriers used in the preferred embodiment of the present invention.

Figure 3: A schematic flow diagram of the method for extracting Pteridium aquilinum extract according to a preferred embodiment of the present invention.

Figure 4: A schematic block diagram of the second preferred embodiment of the present invention with the addition of Pteridium aureum extract.

Fig. 5A: A schematic diagram of the evaluation of the skin of the first use case of the Pteridium parvum extract additive product of the preferred embodiment of the present invention before use.

Fig. 5B: A schematic diagram of the skin evaluation of the Pteridium parvum extract additive product of the preferred embodiment of the present invention after the first use example.

Fig. 6: A schematic diagram of the image of the Pteridium vulgare extract addition product of the preferred embodiment of the present invention adopting the Pteridium vulgare body.

Fig. 7A: A schematic diagram of the evaluation of the skin of the second use case of the Pteridium simonii extract additive product of the preferred embodiment of the present invention before use.

Figure 7B: A schematic diagram of the skin evaluation of the Pteridium parvum extract additive product of the preferred embodiment of the present invention after the second use example.

Fig. 8: A schematic diagram of the activity test of the Pteridium vulgare extract of the Pteridium vulgare extract additive product according to a preferred embodiment of the present invention.

Figure 9: A schematic diagram of the permeability of the Pteridium vulgare extract of the Pteridium vulgare extract additive product according to a preferred embodiment of the present invention.

Figure 10: A schematic diagram of the use of Wound Healing Test to evaluate the cell repair activity of the Pteridium parvum extract as a product added with the Pteridium parvum extract of the preferred embodiment of the present invention.

In order to fully understand the present invention, preferred embodiments are exemplified below and described in detail with the accompanying drawings, and they are not intended to limit the present invention.

The Pteridium vulgare extract and its additive products according to the preferred embodiment of the present invention are applicable to various extracts of Pteridium vulgare and its parts, its additive products and the extraction method thereof, but it is not intended to limit the present invention. The scope of the invention. Furthermore, the Pteris sylvestris extract of the preferred embodiment of the present invention is added and combined in various processed products, such as: preventive or anti-bacterial products, preventive or anti-allergic products, beauty care products, general foods or health foods. It is not intended to limit the scope of application of the present invention.

Figure 1 shows a schematic block diagram of the Pteridium parvum extract additive product according to the first preferred embodiment of the present invention. Please refer to Figure 1. For example, the first preferred embodiment of the present invention uses a storage carrier 10 or a device with similar functions, and the added product contains At least one cosmetic material 2 and at least one Pteridium aurantia extract 3, and the cosmetic raw materials 2 and Pteridium aureum extract 3 are made into a cosmetic composition a.

Please refer to FIG. 1 again. For example, the storage carrier 10 is used to store and carry at least one product assembly, so that an appropriate amount of the product assembly can be taken out of the storage carrier 10 during use. In addition, the storage carrier 10 can be made of various materials (for example: plastic, aluminum thin paper, glass, metal or other suitable materials), and the storage carrier 10 can also be made of a transparent (transparent) material or a semi-transparent (semi-transparent). Material or a non-transparent (opaque) material.

Fig. 2 shows a schematic diagram of the structure of the Pteridium parvum extract additive product and various storage carriers used in the preferred embodiment of the present invention. Please refer to As shown in Figures 1 and 2, for example, the storage carrier 10 is selected from a facial mask [facial mask] 10a, a patch [patch] 10b, a gauze body [gauze] 10c, a bandage roll [bandage] 10d, A capsule (capsule) 10e, a packing bag (packing bag) 10f, a sub-packing box (packing box) 10g, an OK bandage (adhesive bandage) 10h or any combination thereof, and the patch 10b can be selected from a beauty Adhesive skin closure, a sterilization patch or a medical patch, and the storage carrier 10 can also be selected from a surgical tape or a tape with similar functions.

Please refer to Figures 1 and 2. For example, the storage carrier 10 has a first side (first side) and a second side (second side), and the storage carrier 10 includes an outer surface (outer surface). ] And an inner surface (inner surface). The outer surface is located on the first side of the storage carrier 10, and the inner surface is located on the second side of the storage carrier 10. The first side of the storage carrier 10 is an outer side, and the second side of the storage carrier 10 is an inner side.

Please refer to Figures 1 and 2. For example, at least one dispensing space is located on the inner surface of the storage carrier 10, and the dispensing space is used to accommodate the cosmetic composition a or similar materials. And the configuration space can be selected from an airtight space, an unsealable airtight space, a semi-open space or any combination thereof.

Please refer to Figures 1 and 2. For example, the cosmetic material 2 has a first content, and the cosmetic material 2 is selected from a skin care material, a cosmetic material or a perfume material to make a Skin care products, cosmetics or perfume, and the cosmetic material 2 is selected from a powder (powder) material, a cream (cream or ointment) material, or a liquid [liquid] material.

Please refer to Figures 1 and 2. For example, use a The processing equipment 100 (or other equipment with similar functions) adds the Pteridophyta extract 3 to the cosmetic raw material 2, and the Pteridophyllum extract 3 is extracted from a field-collected Pteridium sp. or an artificially cultivated Pteridium sp. , And the Pteridium vulgare is selected from the artificial cultivation Pteridium lichenii [Taiwan native species] of different growth periods.

Please refer to Figures 1 and 2 again. For example, the Pteridium aquilinum extract 3 has a second content, and the Pteridium aquilinum extract 3 has at least one anti-wrinkle ingredient, at least one anti-allergic ingredient, and at least one anti-wrinkle ingredient, at least one anti-allergic ingredient, and at least one anti-wrinkle ingredient. An anti-oxidant ingredient, and the anti-wrinkle ingredient, anti-allergic ingredient and antioxidant ingredient include algae polysaccharides, polyphenols and flavonoids.

Please refer to Figures 1 and 2 again. For example, the Pteridophyte extract 3 is a Pteridophyta extract, a Pteridophyllum extract essential oil, a Pteridophyllum extract, and a Pteridophyllum extract. Extract essence particles, a small leaf fern algae extract essence powder or any combination thereof.

Please refer to Figures 1 and 2, for example, the combination of the cosmetic raw material 2 and the small leaf fern algae extract 3 is arranged in the arrangement space on the inner surface of the storage carrier 10 to form a cosmetic composition. And the storage carrier 10 can be selected from a cosmetic trial bag, a cosmetic bag, a cosmetic box or a cosmetic jar.

Fig. 3 shows a schematic flow diagram of the extraction method of Pteridium aquilinum extract according to a preferred embodiment of the present invention, which is compared with Fig. 1. Please refer to Figures 1 and 3, the method for extracting Pteridium aquilinum extract in a preferred embodiment of the present invention includes step S1: First, for example, it is provided by appropriate technical means (for example: picking operation or cleaning operation) At least one or several small leaf fern bodies 1, and the at least one or several small leaf fern bodies 1 comprise several stems, several contracted upright stems, several globular twigs and several false roots.

Please refer to Figures 1 and 3 again. For example, the small leaf fern body is selected as an artificially cultivated small leaf fern body, and the artificially cultivated small leaf fern body can be selected at a water temperature of 23±3°C or other Appropriate temperature range and sea The water salinity is 32±3ppt or other appropriate salinity range, and it can choose to add trace element culture solution, but it is not used to limit the scope of the present invention.

Please refer to Figures 1 and 3 again. The method for extracting Pteridium aquilinum extract in a preferred embodiment of the present invention includes step S2: Then, for example, in a body of water (such as pure water) by appropriate technical means A homogenizing device (homogenizing device) is used to uniformly crush the small leaf fern algae body 10 to obtain several small leaf fern algae body pieces [pieces].

Please refer to Figures 1 and 3 again. The extraction method of the Pteridium vulgare extract of the preferred embodiment of the present invention includes step S3: Then, for example, the Pteridium lichenii extract is heated in a hot water by appropriate technical means. The body fragments are subjected to extraction operation to obtain a small leaf fern alga body extract, and the temperature of the hot water is about 120°C.

Please refer to Figures 1 and 3 again. The method for extracting the Pteridium aquilinum extract in a preferred embodiment of the present invention includes step S4: Then, for example, the Pteridium aquilinum body extract is cooled by appropriate technical means. To obtain a cooled small leaf fern algae extract, and use a centrifuge [centrifuge] at 10°C or other temperature to perform precipitation and removal of impurities to obtain a supernatant small leaf fern algae extract, which is the leaflet Pteridium algae extract 3.

Please refer to Figures 1 and 3 again. The method for extracting Pteridium aquilinum extract in a preferred embodiment of the present invention includes step S5: Then, for example, use appropriate technical means to extract the supernatant Pteridium aquilinum body extract. Properly perform freeze-drying operation [freeze-drying] to obtain a small leaf fern algae extract powder [powder], and the small leaf fern algae extract powder contains algae polysaccharides [mannose, glucose, galactose or other], more Phenols and flavonoids.

Figure 4 shows a schematic block diagram of the second preferred embodiment of the present invention. Please refer to FIG. 4, compared to the first embodiment, the product containing the Pteridium vulgare extract of the preferred embodiment of the present invention includes at least one food material 20 and at least one Pteridium vulgare extract 3, and the food material 20 and small leaf fern algae extract 3 made into a food composition b.

Please refer to Figure 4 again. For example, the food composition 20 is a beverage, a food, a sauce, a seasoning powder, a health food, a health care product or other products with similar compositions, and The storage carrier 10 can be selected from a product trial package, a product bag, a product box, or a product can.

Figure 5A shows a schematic diagram of the evaluation of the skin of the first use case of the Pteridium parvum extract added product of the preferred embodiment of the present invention before use. Please refer to Fig. 5A. Before using the product with Pteridium aquilinum extract in a preferred embodiment of the present invention, the skin sebum, moisturizing, spots, wrinkles and sensitivity of the first use case are evaluated as requiring enhanced protection (the outermost area) ] Or need preventive protection [sub-peripheral area].

Fig. 5B shows a schematic diagram of the skin evaluation of the Pteridium vulgare extract additive product of the preferred embodiment of the present invention after the first use example. Please refer to Fig. 5B. After using the Pteridium aquilinum extract-added product of the preferred embodiment of the present invention, the moisturizing, wrinkle and sensitivity assessment of the skin of the first use case improved to good [central area].

Fig. 6 shows a schematic diagram of an image of a Pteridium vulgare extract additive product of a preferred embodiment of the present invention using Pteridium vulgare body. Please refer to Figure 6, the preferred embodiment of the present invention adopts the small leaf fern algae extract product to classify based on the length and number of the algae branch, and it contains several stems and numbers. One contracted erect stem, several globular branchlets and several false roots. The algae body of Pteridophyllum has a height of about 15mm, its stem is about 1mm cylindrical, and the top of its erect stem shrinks. The small leaf fern is bright green and grows in a creeping manner, and it has the differentiation of erect stems and stolons, and its erect stem is about 10 to 30 mm high, and its erect stem grows many densely grown branchlets, and its branchlets The top swells into a spherical shape, and its diameter is about 1.0 to 1.5 mm, and its stolon is cylindrical and smooth, and it grows a whisker-like pseudo root downward, and its width is about 0.6 to 1.0 mm.

Figure 7A shows a schematic diagram of the evaluation of the skin of the second use case of the Pteridium parvum extract additive product of the preferred embodiment of the present invention before use. Please refer to Figure 7A. Before using the Pteridophyllum vulgare extract added product of the preferred embodiment of the present invention, the sebum, elasticity, moisturizing, spots and wrinkles of the skin of the second use case are evaluated as requiring enhanced protection (outermost peripheral area) ] Or need preventive protection [sub-peripheral area].

Fig. 7B shows a schematic diagram of the skin evaluation of the Pteridium parvum extract additive product of the preferred embodiment of the present invention after the second use example. Please refer to Fig. 7B. After using the Pteridium vulgare extract added product of the preferred embodiment of the present invention, the moisturizing, elasticity and wrinkles of the skin of the second use case are evaluated to be improved to good [central area].

FIG. 8 shows a schematic diagram of the activity test of the Pteridium vulgare extract of the Pteridium vulgare extract additive product according to a preferred embodiment of the present invention. Please refer to Fig. 8, which is a preferred embodiment of the present invention. The activity test of the Pteridophyte extract of the Pteridophyllum microphylla extract contains collagen level [Collagen level] and collagen degrading enzyme MPP-1 [matrix metalloproteinase] -1, Matrix Metalloprotein-1] performance.

Figure 9 shows a schematic diagram of the permeability of the Pteridium vulgare extract of the Pteridium vulgare extract additive product according to a preferred embodiment of the present invention. Please refer to Fig. 9, which is the preferred embodiment of the present invention. The Pteridium vulgare extract of the product added with the Pteridium vulgare extract increases over time, which can increase its permeability to the skin (normal skin cells).

FIG. 10 shows a schematic diagram of the use of the Pteridium vulgare extract as an added product of the preferred embodiment of the present invention to evaluate the cell repair activity of the Pteridium vulgare extract using a wound healing test. Please refer to Figure 10, the Pteridophyllum vulgare extract of the preferred embodiment of the present invention is added to the product of Pteridium vulgare extract as its concentration increases, which can increase the healing rate of skin wounds, that is, it has cells (such as : Skin cells] repair activity.

As shown in Tables 1 and 2, the fern of the preferred embodiment of the present invention Algae extract added product of Pteridium aquilinum extract is not cytotoxic. As shown in Table 3, the Pteridium parvum L. extract of the preferred embodiment of the present invention has anti-allergic activity.

Table 1. Cytotoxicity of Pteris sphaeroides extract samples to NIH-3T3

Table 2. Cytotoxicity of Pteridium vulgare extract samples to CCD-966SK

Table 3. Anti-allergic activity of Pteris sylvestris extract samples

Relative to the blank control group, * p<0.05, **p<0.01

Each experimental condition has 3 repetitions, and the same experiment is carried out twice. The data is presented as the mean ± standard deviation, and analyzed by Student's t test, the p value is less than 0.05, indicating that there is a significant difference between the test product and the blank control group.

The above-mentioned experimental data are preliminary experimental results obtained under specific conditions, which are only used to easily understand or refer to the technical content of the present invention, and other related experiments are needed. The experimental data and results are not intended to limit the scope of rights of the present invention.

The foregoing preferred embodiments only illustrate the present invention and its technical features. The technology of this embodiment can still be implemented with various substantially equivalent modifications and/or alternatives; therefore, the scope of rights of the present invention shall be subject to a patent application. The scope defined by the scope shall prevail. The copyright in this case is restricted to the use of patent applications in the Republic of China.

1: Small leaf fern algae

10: storage carrier

100: processing equipment

2: Cosmetic raw materials

3: Small leaf fern algae extract

a: cosmetic composition

Claims (10)

A small leaf fern algae extract, which contains: A small leaf fern algae extract is extracted from a small leaf fern algae body, and the small leaf fern algae extract has an anti-wrinkle ingredient, an anti-allergic ingredient and an anti-oxidant ingredient, and the anti-wrinkle ingredient, anti-allergic ingredient and antioxidant ingredient Contains algae polysaccharides, polyphenols and flavonoids. According to the Pteridophyllum microphylla extract described in item 1 of the scope of patent application, the Pteridophyllum microphylla body is selected from different growth stages of Pteridium microphylla. According to the first item of the scope of the patent application, the Pteridium vulgaris extract, wherein the Pteridium vulgare body contains several stems, several contracted upright stems, several globular branchlets and several false roots. According to the first item of the scope of the patent application, the Pteridium aureum extract, wherein the Pteridium aureum extract is not cytotoxic. A small leaf fern algae extract additive product, which contains: At least one cosmetic raw material, which has a first content; and At least one Pteridium vulgare extract having a second content, and the Pteridium vulgare extract is extracted from a Pteridium vulgare, and the Pteridium vulgare extract is added to the cosmetic material to prepare a cosmetic composition Thing Wherein, the Pteridium parvum extract has at least one anti-wrinkle ingredient, at least one anti-allergic ingredient and at least one antioxidant ingredient, and the anti-wrinkle ingredient, anti-allergic ingredient and antioxidant ingredient include algae polysaccharides, polyphenols and flavonoids. According to the fifth of the scope of patent application, the added product of Pteridium aquilinum extract, wherein the cosmetic raw material is selected from a skin care product raw material, a color cosmetics raw material or a perfume raw material to make a skin care product, a color cosmetics product or a perfume . According to the fifth of the scope of patent application, the Pteridium aurantium extract additive product, wherein the cosmetic raw material is selected from a powdered raw material, a cream raw material or a liquid raw material. A small leaf fern algae extract additive product, which contains: At least one food material, which has a first content; and At least one extract of Pteris vulgaris, which has a second content, and the leaf The Pteridium aquilinum extract is extracted from a small Pteridium aquilinum, and the Pteridium aquilinum extract is added to the food material to prepare a food composition; Wherein, the Pteridium parvum extract has at least one anti-wrinkle ingredient, at least one anti-allergic ingredient and at least one antioxidant ingredient, and the anti-wrinkle ingredient, anti-allergic ingredient and antioxidant ingredient include algae polysaccharides, polyphenols and flavonoids. According to the eighth of the scope of patent application, the added product of Pteridium aureum extract, wherein the food composition is a beverage, a food, a seasoning sauce, a seasoning powder, a health food or a health care product. According to the 5 or 8 of the scope of patent application, the Pteridophyllum spp One small leaf fern algae extract granules, one small leaf fern algae extract powder or any combination thereof.

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