TWI698526B - 利用合成之信使ｒｎａ進行之人類誘導型多能幹細胞之無餵養細胞衍化（ｄｅｒｉｖａｔｉｏｎ） - Google Patents

利用合成之信使ｒｎａ進行之人類誘導型多能幹細胞之無餵養細胞衍化（ｄｅｒｉｖａｔｉｏｎ） Download PDF

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TWI698526B
TWI698526B TW104117573A TW104117573A TWI698526B TW I698526 B TWI698526 B TW I698526B TW 104117573 A TW104117573 A TW 104117573A TW 104117573 A TW104117573 A TW 104117573A TW I698526 B TWI698526 B TW I698526B
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cells
reprogramming
mrna
cell
feeder
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TW104117573A
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TW201600605A (zh
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繼武 王
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美商綠陽生物科技及製藥公司
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
    • C—CHEMISTRY; METALLURGY
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/48—Reproductive organs
    • A61K35/54—Ovaries; Ova; Ovules; Embryos; Foetal cells; Germ cells
    • A61K35/545—Embryonic stem cells; Pluripotent stem cells; Induced pluripotent stem cells; Uncharacterised stem cells
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    • C12N2500/00—Specific components of cell culture medium
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C12N2501/602—Sox-2
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/603—Oct-3/4
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C12N2501/604—Klf-4
    • C—CHEMISTRY; METALLURGY
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C12N2501/605—Nanog
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/13—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells
    • C12N2506/1307—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells from adult fibroblasts
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    • C12N2510/00—Genetically modified cells
TW104117573A 2014-05-30 2015-05-29 利用合成之信使ｒｎａ進行之人類誘導型多能幹細胞之無餵養細胞衍化（ｄｅｒｉｖａｔｉｏｎ） TWI698526B (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US14/292,317 US10119150B2 (en) 2012-05-13 2014-05-30 Feeder-free Derivation of human-induced pluripotent stem cells with synthetic messenger RNA
US14/292,317 2014-05-30

Publications (2)

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TW201600605A TW201600605A (zh) 2016-01-01
TWI698526B true TWI698526B (zh) 2020-07-11

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TW104117573A TWI698526B (zh) 2014-05-30 2015-05-29 利用合成之信使ｒｎａ進行之人類誘導型多能幹細胞之無餵養細胞衍化（ｄｅｒｉｖａｔｉｏｎ）

Country Status (7)

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KR (1) KR102361849B1 (fr)
CA (1) CA2950582C (fr)
IL (1) IL249193B (fr)
PH (1) PH12016502343A1 (fr)
SG (1) SG11201609898WA (fr)
TW (1) TWI698526B (fr)
WO (1) WO2015184318A1 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109913494A (zh) * 2019-02-12 2019-06-21 北京呈诺医学科技有限公司 一种新的ips细胞的诱导方法

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012012708A1 (fr) * 2010-07-22 2012-01-26 Regents Of The University Of Minnesota Cellules souches pluripotentes induites

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20120207744A1 (en) * 2009-03-19 2012-08-16 Mendlein John D Reprogramming compositions and methods of using the same
CA2864702A1 (fr) * 2012-02-22 2013-08-29 Amgen Inc. Modeles autologues de mammifere issus de cellules souches pluripotentes induites et procedes associes
US10155929B2 (en) * 2012-05-13 2018-12-18 Allele Biotechnology & Pharmaceuticals, Inc. Feeder-free derivation of human-induced pluripotent stem cells with synthetic messenger RNA

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012012708A1 (fr) * 2010-07-22 2012-01-26 Regents Of The University Of Minnesota Cellules souches pluripotentes induites

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Rosa A1, Brivanlou AH.,Synthetic mRNAs: powerful tools for reprogramming and differentiation of human cells.,Cell Stem Cell.2010 Nov 5;7(5):p.549-550. *

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SG11201609898WA (en) 2016-12-29
KR102361849B1 (ko) 2022-02-11
IL249193B (en) 2020-05-31
IL249193A0 (en) 2017-01-31
CA2950582A1 (fr) 2015-12-03
KR20170023014A (ko) 2017-03-02
WO2015184318A1 (fr) 2015-12-03
TW201600605A (zh) 2016-01-01
CA2950582C (fr) 2020-09-22
PH12016502343A1 (en) 2017-02-13

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