PH12016502343A1 - Feeder-free derivation of human-induced pluripotent stem cells with synthetic messenger rna - Google Patents

Feeder-free derivation of human-induced pluripotent stem cells with synthetic messenger rna

Info

Publication number
PH12016502343A1
PH12016502343A1 PH12016502343A PH12016502343A PH12016502343A1 PH 12016502343 A1 PH12016502343 A1 PH 12016502343A1 PH 12016502343 A PH12016502343 A PH 12016502343A PH 12016502343 A PH12016502343 A PH 12016502343A PH 12016502343 A1 PH12016502343 A1 PH 12016502343A1
Authority
PH
Philippines
Prior art keywords
stem cells
pluripotent stem
induced pluripotent
feeder
human
Prior art date
Application number
PH12016502343A
Other languages
English (en)
Inventor
Wang Jiwu
Original Assignee
Allele Biotechnology & Pharmaceuticals Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US14/292,317 external-priority patent/US10119150B2/en
Application filed by Allele Biotechnology & Pharmaceuticals Inc filed Critical Allele Biotechnology & Pharmaceuticals Inc
Publication of PH12016502343A1 publication Critical patent/PH12016502343A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/48—Reproductive organs
    • A61K35/54—Ovaries; Ova; Ovules; Embryos; Foetal cells; Germ cells
    • A61K35/545—Embryonic stem cells; Pluripotent stem cells; Induced pluripotent stem cells; Uncharacterised stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/98—Xeno-free medium and culture conditions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C12N2501/602—Sox-2
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C12N2501/603—Oct-3/4
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C12N2501/604—Klf-4
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C12N2501/605—Nanog
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/13—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells
    • C12N2506/1307—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells from adult fibroblasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
PH12016502343A 2014-05-30 2016-11-25 Feeder-free derivation of human-induced pluripotent stem cells with synthetic messenger rna PH12016502343A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US14/292,317 US10119150B2 (en) 2012-05-13 2014-05-30 Feeder-free Derivation of human-induced pluripotent stem cells with synthetic messenger RNA
PCT/US2015/033275 WO2015184318A1 (fr) 2014-05-30 2015-05-29 Dérivation sans cellules nourricières, de cellules souches pluripotentes induites humaines, au moyen d'arn messager synthétique

Publications (1)

Publication Number Publication Date
PH12016502343A1 true PH12016502343A1 (en) 2017-02-13

Family

ID=54699891

Family Applications (1)

Application Number Title Priority Date Filing Date
PH12016502343A PH12016502343A1 (en) 2014-05-30 2016-11-25 Feeder-free derivation of human-induced pluripotent stem cells with synthetic messenger rna

Country Status (7)

Country Link
KR (1) KR102361849B1 (fr)
CA (1) CA2950582C (fr)
IL (1) IL249193B (fr)
PH (1) PH12016502343A1 (fr)
SG (1) SG11201609898WA (fr)
TW (1) TWI698526B (fr)
WO (1) WO2015184318A1 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109913494A (zh) * 2019-02-12 2019-06-21 北京呈诺医学科技有限公司 一种新的ips细胞的诱导方法

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010108126A2 (fr) * 2009-03-19 2010-09-23 Fate Therapeutics, Inc. Compositions de reprogrammation et procédés d'utilisation de celles-ci
WO2012012708A1 (fr) * 2010-07-22 2012-01-26 Regents Of The University Of Minnesota Cellules souches pluripotentes induites
CA2864702A1 (fr) * 2012-02-22 2013-08-29 Amgen Inc. Modeles autologues de mammifere issus de cellules souches pluripotentes induites et procedes associes
US10155929B2 (en) * 2012-05-13 2018-12-18 Allele Biotechnology & Pharmaceuticals, Inc. Feeder-free derivation of human-induced pluripotent stem cells with synthetic messenger RNA

Also Published As

Publication number Publication date
TWI698526B (zh) 2020-07-11
IL249193B (en) 2020-05-31
TW201600605A (zh) 2016-01-01
CA2950582C (fr) 2020-09-22
IL249193A0 (en) 2017-01-31
SG11201609898WA (en) 2016-12-29
CA2950582A1 (fr) 2015-12-03
WO2015184318A1 (fr) 2015-12-03
KR102361849B1 (ko) 2022-02-11
KR20170023014A (ko) 2017-03-02

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