TWI648053B - Method for producing oil-soluble placenta extract - Google Patents

Abstract

An object of the present invention is to provide a method for producing an oil-soluble placenta extract which comprises a phosphorus-containing component and can be blended in an oily cosmetic or a quasi-drug.

The solution comprises: chopping step 20, chopping the placenta; heating step 30, after chopping step 20, heating the chopped placenta; freezing step 40, after heating step 30, freezing the placenta Drying; powdering step 50, after freeze-drying step 40, the freeze-dried placenta is powdered; extraction step 60, after the powdering step 50, adding ether to the powdered placenta for extraction; Step 70, after the extraction step 60, removing the ether separation from the extract; and drying the step 80 under reduced pressure, after removing the step 70, drying the extract under reduced pressure to obtain a dried product containing the phosphorus-containing component. And the oil dissolving step 90, after the vacuum drying step 80, dissolving the dried product in the oil.

Description

 Method for producing oil-soluble placenta extract  

The present invention relates to a method for producing an oil-soluble placenta extract which can be blended into an oily cosmetic or quasi-drug such as a foundation, a lipstick, an emollient oil or a cleansing agent.

Placental extracts are widely used as cosmetic raw materials from placenta. However, most of the placenta blended with commercially available quasi-drugs or cosmetics is a water-soluble extract. The water-soluble placenta extract cannot be incorporated into oily dosage forms such as lipsticks, foundations, and emollient cosmetics.

Herein, Patent Document 1 discloses an incomplete rejuvenation nutritional supplement comprising: a placental extract, an extract from a placenta; a fat; and an additive selected from the group consisting of collagen, algae, plant extract, and royal jelly. The class consists of at least one of the groups.

Further, Patent Document 2 discloses a hair styling composition comprising a hydrophobic cerium oxide, a dispersion for dispersing hydrophobic cerium oxide, and a hair growth component; wherein it is described that a placental extract can be used as hair growth Ingredients, and the hair growth component can be made into an oil-soluble substance.

Further, Patent Document 3 discloses a cosmetic composition containing (A) selected from the group consisting of Morus Nigra Leaf, Magnolia Biondii Flower, Trifolium Pratense, and White Lupin Seeds. (Lupinus Albus Seed), Hypericum Perforatum, Origanum Vulgare Flower, Viola Tricolor, Centaurea Cyanus Flower, Melissa Officinalis, Calendula Officinalis Flower) one or more extracts and/or (B) purified purified oil and/or (C) whitening component by column adsorption; wherein olive oil can be used As an oil agent, a placental extract can be used as a whitening ingredient.

Further, Patent Document 4 discloses a percutaneous absorption type pregnancy line formation inhibitor which contains whey, a fibroblast formation promoting component, and an oil agent; wherein it is described that a placental extract can be used as a fibroblast growth promoting component. .

[Previous Technical Literature]

[Patent Literature]

[Patent Document 1] Japanese Laid-Open Patent Publication No. 2016-113442

[Patent Document 2] International Publication No. 2012/105096

[Patent Document 3] Japanese Patent Laid-Open Publication No. 2008-50292

[Patent Document 4] Japanese Patent Laid-Open Publication No. 2002-145788

Although Patent Document 1 to Patent Document 4 describe the blended oil and the placenta extract, there is no description about the specific method for producing the oil-soluble placenta extract.

Accordingly, the present invention is directed to a method for producing an oil-soluble placental extract comprising a phosphorus-containing component which can be incorporated into an oily cosmetic or quasi-drug.

The method for producing an oil-soluble placenta extract of the present invention according to claim 1, comprising: a chopping step of chopping the placenta; and a heating step of heating the chopped placenta after the chopping step a lyophilization step of lyophilizing the placenta after the heating step; a pulverization step of lyophilizing the placenta after the lyophilization step; and an extraction step of pulverizing After the step, the ether is added to the powdered bead plate for extraction; the removal step is performed after the extraction step, and the ether separation is removed from the extract; the vacuum drying step is performed after the removing step, and the extraction is performed. The liquid is dried under reduced pressure to obtain a dried product containing a phosphorus-containing component; and an oil-dissolving step is performed by dissolving the dried product in an oil after the vacuum drying step.

The present invention according to claim 2 is the method for producing an oil-soluble placenta extract according to claim 1, wherein in the extracting step, the extraction temperature is set to 20 ° C or higher and 60 ° C or lower, and the ether to be added is added. Amount of powder The weight of the aforementioned placenta of the finalization is 5 times the amount to 10 times the weight.

The present invention according to claim 3 is the method for producing an oil-soluble placenta extract according to claim 2, wherein in the extracting step, the extraction temperature is set to 40° C. or higher and 60° C. or lower, and the extraction time is set to 2 hours or less.

The present invention is the method for producing an oil-soluble placenta extract according to any one of claims 1 to 3, wherein in the heating step, the heating temperature is 110 ° C or more and 135 ° C or less. In the oil dissolving step, the ratio of the dried product dissolved in the oil is 0.1% or more and 5.0% or less.

According to the present invention, there can be provided a process for producing an oil-soluble placental extract comprising a phosphorus-containing component which can be blended in an oily cosmetic.

10‧‧‧Placenta selection steps

20‧‧‧Chopping steps

30‧‧‧heating steps

40‧‧‧ freeze drying step

50‧‧‧Powdering step

60‧‧‧ Extraction step

70‧‧‧ removal steps

80‧‧‧Decompression drying step

90‧‧‧ oil dissolution step

Fig. 1 is a view showing a manufacturing step of the oil-soluble placenta extract of the present embodiment.

Fig. 2 is a view showing the step of producing the oil-soluble placenta extract, wherein the heating temperature in the heating step is 120 ° C, and the heating time is 20 minutes or longer and 30 minutes or shorter, and the freeze-dried placenta is in the extraction step. A graph of the amount of the phosphorus-containing component at each extraction temperature and extraction time at a weight of 5 times (Fig. 2 (a)) or 10 times (Fig. 2 (b)).

Fig. 3 is a graph showing the results of an evaporation inhibition effect test of skin moisture when the oil-soluble placental extract was blended in a gel at 3%.

[Formation of the Invention]

The method for producing an oil-soluble placenta extract according to the first embodiment of the present invention comprises: a chopping step of chopping the placenta; a heating step of heating the chopped placenta after the chopping step; and a freeze-drying step After the heating step, the placenta is freeze-dried; the powdering step is after the freeze-drying step, the freeze-dried placenta is powdered; the extraction step is after the powdering step, adding ether to the powdered placenta The extraction step is performed after the extraction step, and the ether separation is removed from the extract; the vacuum drying step is performed after the removal step, and the extract is dried under reduced pressure to obtain a dried product containing the phosphorus component. And the oil dissolving step, after the vacuum drying step, dissolving the dried product in the oil.

According to the present embodiment, an oil-soluble placental extract containing a phosphorus-containing component of a predetermined amount or more can be produced. Further, by performing heat treatment in the heating step before the freeze-drying step to change the structure of the placenta, the extraction efficiency of the phosphorus-containing component can be improved.

In the second embodiment of the present invention, in the method for producing an oil-soluble placenta extract according to the first embodiment, in the extraction step, the extraction temperature is set to 20° C. or higher and 60° C. or lower, and the amount of the ether to be added is added. The weight of the aforementioned placenta was pulverized by 5 times to 10 times by weight.

According to this embodiment, the phosphorus-containing component can be extracted more efficiently.

In the third embodiment of the present invention, in the method for producing an oil-soluble placenta extract of the second embodiment, the extraction temperature is 40° C. or higher and 60° C. or lower, and the extraction time is 2 hours or shorter.

According to this embodiment, the phosphorus-containing component can be extracted more efficiently, and deterioration of the phosphorus-containing component can be avoided.

According to a fourth aspect of the present invention, in the method for producing an oil-soluble placenta extract according to any one of the first to third embodiments, in the heating step, the heating temperature is 110° C. or higher and 135° C. or lower, and the oil is dissolved. In the step, the ratio of the dried product dissolved in the oil is 0.1% or more and 5.0% or less.

According to this embodiment, an oil-soluble placental extract containing 0.1 to 0.6% of a phosphorus-containing component can be produced.

[Examples]

Hereinafter, a method for producing an oil-soluble placenta extract according to an embodiment of the present invention will be described.

Fig. 1 is a view showing a manufacturing step of the oil-soluble placenta extract of the present embodiment.

The method for producing the oil-soluble placenta extract of the present embodiment has a placenta selection step 10, a chopping step 20, a heating step 30, a freeze-drying step 40, a powdering step 50, an extraction step 60, a removing step 70, and a vacuum drying step. 80, and oil dissolution step 90.

First, in the placenta selection step 10, a placenta as a raw material for the oil-soluble placenta extract is selected.

The placenta can use a placenta of a mammal such as a pig, a horse, a sheep, etc., wherein since the placenta safety of the pig is particularly excellent, in the present embodiment, the placenta of the pig is selected.

Next, in the chopping step 20, the placenta selected from the placenta selection step 10 is used to remove the skin tissue, and the pile tissue is chopped to form a ground meat.

Next, in the heating step 30, the chopped placenta is heated in the chopping step 20.

In the heating step 30, a high-pressure vapor which is formed at a temperature of 110 ° C or more and 135 ° C or less in a high pressure environment is used. The heating using such high-pressure steam can be, for example, a device which is heated while being pressurized by an autoclave or the like.

The extraction efficiency of the phosphorus-containing component in the extraction step 60 can be improved by the heat treatment in the heating step 30.

Further, although the oil-soluble placenta extract is an extract derived from a natural product, it is not possible to set a sterilization step such as heating or filtration in a subsequent step like a water-soluble placenta extract. Therefore, it is preferable to set the heating temperature in the heating step 30 to 110° C. or more and 135° C. or less, and to set the heating time to 15 minutes or more and 1 hour or less to serve as a suitable sterilization treatment for the placenta. Further, from the viewpoint of the efficiency of the heat treatment, the heating temperature is preferably 120° C. or higher and 122° C. or lower, and the heating time is set to 20 minutes or longer and 30 minutes or shorter.

Next, in the freeze-drying step 40, the heated placenta in the heating step 30 is freeze-dried.

By freeze drying, the water content of the placenta will become lower. By preliminarily reducing the water content of the placenta before the extraction step 60, it is easier to carry out the separation operation other than the ether separation in the step 70.

Next, in the powdering step 50, the placenta which has been freeze-dried in the freeze-drying step 40 is powdered by pulverization or the like.

By bringing the placenta into a powder, the contact efficiency with ether can be improved.

Next, in the extraction step 60, extraction is carried out by adding ether to the powdered placenta in the powdering step 50. As the ether, diethyl ether is preferably used from the viewpoint of extractability of the phosphorus-containing component.

In the present embodiment, for the case where a weight of 5 times the amount of diethyl ether is added to the lyophilized placenta, and a case where the lyophilized placenta is added with a weight of 10 times of diethyl ether, the following conditions are carried out. extraction.

[Condition 1] Weight 5 times, extraction temperature: normal temperature (25 ° C), extraction time: 2 hours

[Condition 2] Weight 5 times, extraction temperature: normal temperature (25 ° C), extraction time: 16 hours

[Condition 3] Weight 5 times, extraction temperature: heating (55 ° C), extraction time: 2 hours

[Condition 4] Weight 10 times, extraction temperature: normal temperature (25 ° C), extraction time: 2 hours

[Condition 5] Weight 10 times, extraction temperature: normal temperature (25 ° C), extraction time: 16 hours

[Condition 6] Weight 10 times, extraction temperature: heating (55 ° C), extraction time: 2 hours

Further, since diethyl ether has a boiling point of 36.4 ° C at atmospheric pressure, when it is set to a temperature higher than this, a liquid state of diethyl ether is maintained by blowing nitrogen gas into a pressurized state.

The heating is carried out by indirect heating using steam. In order to avoid discoloration and deterioration of the phosphorus-containing component, the extraction time is set to 2 hours or less during heating. Further, the lower limit of the extraction time depends on the extraction temperature or the amount of diethyl ether to be added, etc., but is preferably 30 or more, more preferably 1 hour or more. Further, the higher the extraction temperature, the higher the extraction efficiency, but if the extraction temperature is too high, the phosphorus-containing component may be discolored and deteriorated. Therefore, the extraction temperature during heating is preferably 40 ° C or more and 60 ° C or less, more preferably 40 ° C. Above 55 ° C.

Next, in the removing step 70, the diethyl ether separated from the extract obtained by the extraction in the extraction step 60 is removed.

By reducing the water content of the placenta in advance by freeze-drying the placenta in the freeze-drying step 40, it is possible to remove the diethyl ether by a simple method such as filtration.

Next, in the vacuum drying step 80, the filtrate obtained by the filtration of the removal step 70 is dried under reduced pressure.

Thereby, diethyl ether is removed, and a paste-like dried product containing a phosphorus-containing component can be obtained.

Fig. 2(a) is a view showing that the heating temperature in the heating step is 120 ° C, the heating time is 20 minutes or more and 30 minutes or less, and when the freeze-dried placenta is added in the extraction step by a 5-fold weight of diethyl ether. A plot of the amount of phosphorus component for each extraction temperature and extraction time. Fig. 2(b) is a view showing that the heating temperature in the heating step is 120 ° C, the heating time is 20 minutes or more and 30 minutes or less, and when the freeze-dried placenta is added in the extraction step by 10 times by weight of diethyl ether. A plot of the amount of phosphorus component for each extraction temperature and extraction time.

In the second drawing, the highest value of the phosphorus-containing component of the paste-dried product obtained in the vacuum drying step is expressed as "100".

As shown in Fig. 2, it was found that when extraction was carried out at room temperature (25 ° C), the conditions of extracting the lyophilized placenta by adding 10 times the weight of diethyl ether for 16 hours (condition 5) were most efficient; When the extraction was carried out at 55 ° C, the conditions of extracting the lyophilized placenta by adding 5 times the weight of diethyl ether for 2 hours (condition 3) were the most efficient.

Further, under heating conditions, since it can be extracted with a small amount of a solvent (diethyl ether), it is a better condition in terms of the worker's health or environmental burden.

Further, in the extraction under normal temperature conditions, the amount of the phosphorus-containing component which is almost the same as that under the heating condition can be obtained by extending the extraction time. Therefore, the extraction under heating conditions or the room temperature can be selected according to the manufacturing environment of the manufacturing equipment or the like. Extraction under conditions.

Here, as a comparative example, after the chopping step 20, the heating step 30 is omitted and the process proceeds to the freeze-drying step 40, and ether is added to the placenta powdered by the powdering step 50 to carry out extraction of the phosphorus-containing component. The extraction conditions were set to Condition 5 (weight 10 times, extraction temperature: normal temperature (25 ° C), extraction time: 16 hours).

As a result, in the above-described embodiment, in the heating step 30, the heating temperature is 120 ° C, and the heating time is 20 minutes or more and 30 minutes or less, and the heat treatment is performed, and the freeze drying step 40, the powdering step 50, and the extraction step are performed. When the highest value in the case of extracting 10 times of diethyl ether in 60 is "100", the amount of the phosphorus component is "64".

In the embodiment, in the heating step 30, the heating temperature is 120 ° C, the heating time is 20 minutes or more and 30 minutes or less, and the heat treatment is performed, and the freeze drying step 40, the powdering step 50, and the extraction step 60 are carried out under the conditions. 5 When the extraction was carried out, as shown in Fig. 2, the amount of the phosphorus-containing component was "97". Therefore, it was found that the extraction efficiency of the phosphorus-containing component was increased by about 1.5 times by the heating step 30.

Next, in the oil dissolving step 90, the paste-dried dried product obtained in the reduced-pressure drying step 80 is dissolved in oil.

The oil is preferably olive oil or sesame oil, but other oils may be used.

After dissolving the paste-dried product in olive oil, if there is a precipitate, it is preferably removed by filtration or the like, and the oil-soluble placenta extract is completed.

In the oil dissolving step 90, the ratio of the dried product dissolved in the oil is preferably 0.1% or more and 5.0% or less. Thereby, the amount of the phosphorus-containing component contained in the oil-soluble placenta extract can be made 0.1 to 0.6%.

Moreover, when the ratio of the dry matter dissolved in the oil is 0.5% or more and 2.0% or less, the amount of the phosphorus-containing component contained in the oil-soluble placental extract can be made 0.2 to 0.5%.

Further, the analysis of the phosphorus-containing component was carried out by dry-ashing the sample using an ICP emission spectrometer (manufactured by Agilent Technologies, Inc.), followed by extraction with dilute hydrochloric acid.

In order to investigate the moisturizing effect, the oil-soluble placenta extract obtained by the above-described production method was tested for the evaporation inhibition effect of the skin moisture when it was blended at 3%. The results are shown in Figure 3. At this time, the skin moisture content in the general case (when no substance was applied) was set to 0 μS. Further, the test conditions were a temperature of 25 ° C and a humidity of 30%; and the measurement was carried out using a skin surface water content measuring device (SKICON-200EX).

As shown in Fig. 3, it is known that the oil-soluble placental extract exhibits a higher moisturizing power than glycerin, squalane, and a water-soluble placenta extract.

[Industrial availability]

The oil-soluble placenta extract of the production method of the present invention can be blended with an oily cosmetic or quasi-drug such as a foundation, a lipstick, an emollient oil, a cleansing agent or the like.

Claims (4)

A method for producing an oil-soluble placenta extract, characterized by having a chopping step of chopping a placenta; a heating step of heating the chopped placenta after the chopping step; and a freeze-drying step After the heating step, the placenta is freeze-dried; the powdering step is performed after the freeze-drying step, and the freeze-dried the placenta is powdered; and the extracting step is performed after the powdering step, and the powdering is performed. Adding ether to the placenta, extracting the extraction temperature to 20° C. or higher and 60° C. or less; and removing the step of removing the ether from the extract after the extraction step; and drying the step under the step of removing the pressure. Thereafter, the extract is dried under reduced pressure to obtain a dried product containing a phosphorus-containing component, and an oil-dissolving step is performed after the vacuum-drying step, and the dried product is dissolved in oil. The method for producing an oil-soluble placenta extract according to claim 1, wherein in the extracting step, the amount of the ether to be added is from 5 times the weight of the aforementioned placenta to 10 times the weight. The method for producing an oil-soluble placenta extract according to claim 2, wherein in the extracting step, the extraction temperature is set to 40 ° C or higher. Below °C, and the extraction time is set to 2 hours or less. The method for producing an oil-soluble placenta extract according to any one of claims 1 to 3, wherein in the heating step, the heating temperature is set to be 110 ° C or higher and 135 ° C or lower, and in the oil dissolving step, The ratio of the dried product dissolved in the oil is 0.1% or more and 5.0% or less.