KR102410486B1 - Health functional food composition for improving skin condition comprising fish collagen and complex extract, and manufacturing method thereof - Google Patents

Abstract

The present invention is fish collagen; and a complex extract of Centella asiatica, rosemary, rooibos and camellia leaves; relates to a health functional food composition for skin improvement and a method for manufacturing the same, wherein the health functional food composition for skin improvement of the present invention promotes collagen synthesis and inhibits tyrosinase Since the effect is excellent, it can be usefully used for skin improvement purposes such as improvement of wrinkles and/or elasticity of the skin, and skin whitening.

Description

Health functional food composition for skin improvement comprising fish collagen and complex extract, and manufacturing method thereof

The present invention relates to a health functional food composition for skin improvement comprising fish collagen and a complex extract, and a method for preparing the same.

In the human skin, due to aging due to weight loss or increase in age, the secretion of various hormones involved in metabolism internally decreases, and at the same time, the function and activity of immune cells are reduced, so that the immunity and living body necessary for the living body are reduced. Protein biosynthesis is reduced, and externally, damage is caused to the skin due to ultraviolet rays and environmental pollution, which causes side effects such as decreased skin elasticity, decreased skin gloss, wrinkles, spots, freckles, and increased age spots. Elements cause deterioration of skin function and loss of visual beauty.

It has been reported that the reduction of skin wrinkles and skin elasticity is generally caused by several factors such as moisture content and collagen content of the skin, and collagen has the greatest influence among them.

Collagen is a fibrous tissue that gives the skin flexibility and is a major component that accounts for about 70% of the dermal tissue. Polypeptide chains of collagen fibers are synthesized with peptides (telopeptide) at both ends in the granular plasma reticulum of fibroblasts, and then secreted out of the cell in the form of procollagen combined in a triple helix structure. In the secreted procollagen, the terminal peptide is cleaved by a specific enzyme to produce tropocollagen, which is combined with each other in the extracellular matrix to be synthesized into collagen fibers.

The synthesis of these collagen fibers is rapidly reduced as skin aging progresses, and as the collagen fibers are deformed by the external environment, wrinkles and loss of elasticity occur in the skin.

On the other hand, with the development of medical technology and the improvement of living standards, the desire for beauty is getting stronger, and the perception that appearance itself is a social competitiveness is also increasing.

A person's skin color is determined by several complex factors such as season, race, and sex, and it is known that it is mainly determined by the amounts of melanin, carotene, and hemoglobin, of which an important factor is melanin.

The melanin is a phenolic macromolecular natural pigment widely present in living organisms. In the human body, it is synthesized from melanosomes in melanocytes in the basal layer of the epidermis, and metastasized to surrounding keratinocytes to represent the color of human skin.

Excessive production of melanin can cause darkening of the skin color and further various skin diseases including acquired hyperpigmentation such as melasma, postinflammatory melanoderma, and solar lentigo. can

Conventionally, products for skin improvement have been mainly developed in the form of cosmetics, and studies to improve the skin with functional foods by combining various ingredients are also in progress, but there is a problem that the effect is unclear or the effect is insignificant even when taken for a long time.

Therefore, there is a need to develop functional foods using natural products that are safe and have excellent collagen synthesis and whitening effects, which can be usefully used for skin improvement.

Korean Patent Publication No. 10-2021-0075256

The present inventors have recognized the problems of the prior art, and have demonstrated that a health functional food composition comprising fish collagen and a specific extract exhibits an excellent collagen synthesis effect and a tyrosinase activity inhibitory effect, thereby demonstrating that it can be usefully used for skin improvement purposes. came to the conclusion of the invention.

The present invention is fish collagen; and a complex extract of Centella asiatica, rosemary, rooibos and camellia leaves; relates to a health functional food composition for skin improvement comprising.

The present invention relates to a method for preparing the health functional food composition for skin improvement.

Hereinafter, this will be described in detail. All combinations of the various elements disclosed herein are within the scope of the present invention. In addition, it cannot be considered that the scope of the present invention is limited by the following specific description.

The present invention is fish collagen; and a complex extract of Centella asiatica, rosemary, rooibos and camellia leaves; provides a health functional food composition for skin improvement comprising.

In the present invention, "fish collagen" means collagen obtained from fish. For example, it may be collagen obtained from fish scales, fish skins, etc.

In the present invention, the fish collagen powder may be a fish scale collagen powder. Fish scale collagen powder has a very small molecular weight of 300 to 1,000 Da and is easily absorbed by the human body, thereby providing excellent effects on the skin.

According to embodiments of the present invention, the fish collagen may have a molecular weight of 300 to 1,000 Da, preferably 500 to 1,000 Da, but is not limited thereto.

In the present invention, "Centella asiatica" is also referred to as tiger grass, clam, etc., and mainly inhabits in humid areas such as southern islands of the Korean Peninsula and has scale-like leaves with a diameter of 2 to 5 cm. it is flesh In Korea, the outpost of Centella asiatica is used for medicinal purposes, and it is known that it has the effects of cooling heat, dampness, small bells and detoxification.

In the present invention, "Rosemary (Rosmarinus officinalis L.)" has a strong herbal scent and has been used as perfume and medicine since ancient times, and is also widely used as a spice for food as a deodorant and preservative. In addition, it has antibacterial action and strong antioxidant action.

The main components of rosemary include carnosic acid, carnosol, etc., and by providing hydrogen atoms to active free radicals to make stable non-radicals, it exhibits an antioxidant effect that removes active oxygen.

In the present invention, "rooibos (Aspalathus linearis)" refers to a coniferous tree belonging to the legume family, which grows in the alpine region of 450 m or more located west of Cape Town, South Africa. It is mainly used as tea by drying the leaves. It is sweet, has no caffeine, has an extremely low tannin concentration, and is known to have antioxidant properties.

In the present invention, "Camellia japonica" is a medicinal plant belonging to the family Theaceae, which is native to China and Japan, including Korea, and about 200 species are known. Among them, about 70 species are related to the subgenus Camellia, that is, Camellia japonica and C. sasanqua. The camellia tree native to Korea is one species (Camellia japonica L.) and is an arboreal tree of the family Theaceae and the genus Camellia. Camellia leaves are effective for psoriasis, sore throat, and burns, and branches and fruits are effective for dandruff, blood loss, epistaxis, eosinophilia, chondrosis, menstrual abnormalities, diuresis, sore throat, intestinal bleeding, and poisoning.毒), bruises, hematemesis and hemoptysis, haemostatic, and burns are known to be effective. In addition, it has been reported that camellia leaves and flowers contain triterpenes, flavonoids, tannins and fatty acids with antibacterial, antioxidant, and anti-inflammatory activities.

In the present invention, the camellia extract may be an extract extracted from one or more selected from the group consisting of leaves, stems, flowers, roots, fruits, seeds, and mixtures of camellia leaves, preferably extracts of camellia leaves. can

Centella asiatica, rosemary, rooibos and camellia leaves used as raw materials for the extract in the present invention may be used without limitation, those collected from nature, directly grown, or commercially available (including processed ones).

In the present invention, "extract" means the result of extraction using an appropriate extraction solvent and extraction method, and the extract is an extract obtained by extraction, a diluted or concentrated solution of the extract, a dried product obtained by drying the extract, a prepared product thereof, or purified products, or fractions.

In the present invention, "complex extract" refers to an extract extracted through a predetermined manufacturing process in a mixture state in which the raw materials of each extract are mixed at the same time.

According to embodiments of the present invention, the raw material for preparing the complex extract may be in a state in which centella asiatica, rosemary, rooibos and camellia leaves are crushed and evenly mixed.

According to embodiments of the present invention, the complex extract may be extracted from a raw material mixture obtained by mixing Centella asiatica, rosemary, rooibos and camellia leaves in a weight ratio of 2:2:1:1, and the weight ratio is that of the complex extract. It may be for showing an excellent effect by the active ingredient and a synergistic effect with fish collagen.

According to embodiments of the present invention, the health functional food composition may include the fish collagen and the complex extract in a weight ratio of 2: 1, and the weight ratio provides excellent effects and fish by the active ingredient of the health functional food composition. It may be for showing a synergistic effect with collagen.

In the present invention, "active ingredient" means a component that can exhibit the desired activity (effect) alone or with a carrier that has no activity by itself, and can be used interchangeably with the active ingredient in the present specification. .

The complex extract of Centella asiatica, rosemary, rooibos, and camellia leaves contained in the health functional food composition of the present invention may be extracted and mixed by a specific manufacturing method, thereby enhancing the skin improvement effect.

In the present invention, the complex extract may be extracted by a method comprising the following steps:

S1) pulverizing a raw material mixture of Centella asiatica, rosemary, rooibos and camellia leaves, and adding 80% (w/v) ethanol to the pulverized raw material mixture, followed by stirring to homogenize;

S2) ultrasonically extracting the homogenized raw material mixture solution by ultrasonication at 60 to 80° C. for 2 to 4 hours;

S3) separating the sonicated solution into a filtrate and a residue by filtering;

S4) adding purified water to the separated residue, followed by stirring to homogenize;

S5) heat-treating the homogenized solution at 130 to 150° C. for 2 to 4 hours to extract hot water;

S6) filtering the heat-treated solution to separate it into a filtrate and a residue; and

S7) mixing the filtrate separated in steps S3 and S6.

Hereinafter, each step of the complex extract manufacturing method will be described in detail.

The step S1 in the complex extract manufacturing method of the present invention is a step of preparing a raw material mixture solution by grinding the raw material mixture, adding a solvent, and stirring and homogenizing.

In step S1, the solvent is preferably 80% (w/v) ethanol for ultrasonic extraction to be performed following step S1, and when purified water is used, the yield of the active ingredient to be extracted may be low.

In step S1, the raw material mixture may be in a frozen state. That is, in step S1 of the present invention, the raw material mixture may be prepared in a frozen state and pulverized through a grinder.

The pulverization of step S1 may be performed to have a size of 10 to 500 μm, and preferably may be performed to have a size of 400 to 500 μm.

When pulverized to a size smaller than the above range, time and energy efficiency may be reduced in the process of pulverizing the raw material mixture in a frozen state. Therefore, the intended effect of the present invention may not be sufficiently exhibited.

In addition, the raw material mixture in step S1 may be subjected to a pretreatment step including the following steps before pulverization:

a) Washing and mixing Centella asiatica, rosemary, rooibos and camellia leaves, adding purified water and leaving it for 10 to 20 minutes, then removing the water;

b) freezing the water-removed mixture at -40 to -50°C and then leaving it at room temperature for 3 to 4 hours; and

c) Freezing the left mixture at -40 to -50°C after drying.

The shredding in step a) may be performed so that the size of the raw material is 0.1 to 1.0 cm, and preferably 0.3 to 0.7 cm. According to embodiments of the present invention, the slicing may be performed to be about 0.5 cm, but is not limited thereto.

Step a) is to immerse (permeate) the purified water into each raw material, and it is sufficient to leave it for a time for the purified water to be sufficiently absorbed into the raw material mixture, but it may be preferable to leave it for 10 to 20 minutes for manufacturing efficiency. have.

"Remove water" in step a) means removing the purified water existing outside the mixture, for example, it may be to remove the purified water existing outside the mixture by passing through a filter, but is not limited thereto . In addition, it may mean to remove 90% to 99.9%, preferably 95% to 99.9% of purified water existing outside the mixture, but is not limited thereto.

Step b) is a step of performing freezing and thawing on the raw material mixture immersed in purified water, and according to embodiments of the present invention, step b) is repeated three or more times, and it may be preferable to be continuously performed, , more preferably 3 to 5 times, more preferably 4 or 5 times, and may be continuously performed, but is not limited thereto.

When it is carried out a number of times less than the above range, the effect of increasing the extraction of the active ingredient due to the pretreatment may be insignificant, and when it is performed more times than the range, the effect of increasing the extraction of the active ingredient compared to time and energy consumption may not be large. It is not suitable for manufacturing efficiency.

In the experimental example to be described later, when step b) is repeatedly performed three or more times, it is specifically demonstrated that the skin improvement effect due to the composition can be greatly improved. It was confirmed that the extraction amount could be increased.

In the method for producing a complex extract of the present invention, step S2 is a step of ultrasonically extracting the homogenized raw material mixture solution.

The step S2 is carried out at 60 to 80 ℃, below the temperature range, extraction of the components included in the raw material mixture may not sufficiently occur, and if it exceeds the above range, the extraction yield may increase and the extraction time may be shortened. , there is a problem that the active ingredient contained in the raw material mixture may be denatured by heat. Therefore, in consideration of both the extraction yield and integrity of the active ingredient, the temperature in the above range is very advantageous.

Step S2 may be performed for 2 to 4 hours, and according to embodiments of the present invention, may be performed for about 3 hours, but is not limited thereto.

If the extraction is less than the time range, the extraction of the active ingredient contained in the raw material mixture may not sufficiently occur, and if it exceeds the above range, the extraction yield of the active ingredient relative to the time may be lowered, which may not be appropriate in terms of manufacturing efficiency.

In addition, when the ultrasonic extractor is irradiated with a focus on a specific position, the temperature of the medium may rise and the active ingredient contained in the raw material may be destroyed or denatured. In addition, the stirring may be performed by a stirring device provided in the ultrasonic irradiation device, or may be performed through a separately provided stirring device.

In the method for producing a complex extract of the present invention, steps S3 and S6 are steps of separating the residue and the filtrate containing the active material by ultrasonic extraction, and the separation may be performed by a known method.

In the method for manufacturing a complex extract of the present invention, steps S4 and S5 are steps of hot water extraction of the residue remaining after separation in step S3.

In step S5, the solvent is preferably purified water, and when the organic solvent is used, the extraction yield of the active ingredient to be extracted remaining in the residue may be low.

The step S5 may be performed at 130 to 150° C., and may be performed for 2 to 4 hours.

Below the temperature range, extraction of the components contained in the residue may not sufficiently occur, and if it exceeds the above range, the extraction yield may be increased and the extraction time may be shortened, but the active ingredient contained in the residue may be denatured by heat The problem is that it could be. Therefore, in consideration of both the extraction yield and integrity of the active ingredient, the temperature in the above range is very advantageous.

In addition, if the extraction is less than the time range, the extraction of the active ingredient contained in the residue may not sufficiently occur, and if it exceeds the above range, the extraction yield of the active ingredient relative to the time may be lowered, which may not be appropriate in terms of manufacturing efficiency. have.

Between the steps S2 and S3 in the complex extract manufacturing method of the present invention; and cooling the extract obtained between steps S5 and S6 to 2 to 6° C. and leaving (aging) for 4 to 6 hours, thereby increasing the skin improvement effect of the extracted active ingredient effect can be exhibited.

In the complex extract manufacturing method of the present invention, step S7 is a step of mixing the filtrate separated in steps S3 and S6, and the component maximally extracted from the raw material by a manufacturing method in which different extraction methods are continuously and sequentially performed. is a mixing step.

In the complex extract manufacturing method of the present invention, it may be advantageous to perform steps S1 to S7 continuously and sequentially in maximally extracting the active ingredient from the raw material. In this case, it was specifically demonstrated that the skin improvement effect can be increased due to collagen production and tyrosinase inhibitory activity.

In the present invention, "skin improvement" means, for example, improving skin elasticity, reducing wrinkles, whitening, etc. generally known to improve the quality of the skin.

In the present invention, "health functional food" refers to a food manufactured using raw materials or ingredients that are easily deficient in daily meals or have a useful function in the human body, and refers to food that helps maintain human health, but is limited thereto. This includes all health foods in the ordinary sense.

In the present invention, the health functional food may be mixed with food additives in the form of tablets, capsules, pills, granules, liquids, powders, flakes, syrups, gels, jellies, bars, films, etc. to be commercialized as health functional food.

Food additives that can be mixed in the health functional food include, for example, flavoring agents, sweeteners, flavoring agents, preservatives, natural carbohydrates, nutrients, vitamins, electrolytes, colorants, pextan and salts thereof, alginic acid and salts thereof, It may be an organic acid, a protective colloidal thickener, a pH adjuster, a stabilizer, a preservative, glycerin, alcohol, etc., but is not limited thereto, and if it can be added to food, it can exhibit a beneficial effect on the health functional food composition of the present invention may be included without limitation.

In addition, the health functional food composition of the present invention, in addition to the active ingredient, has already been verified in the art for safety and has been verified in the art to increase/reinforce the skin improvement effect, and to enhance the convenience of taking or ingestion through the addition of similar activity. It may further include any compound or natural extract known to have activity. Such compounds or extracts include compounds, extracts, and pharmaceuticals listed in compendial documents such as pharmacopeias of each country (“Korea Pharmacopoeia” in Korea) and health functional food regulations of each country (“health functional food standards and specifications” announced by the Ministry of Food and Drug Safety in Korea). Each country's laws governing the manufacture and sale of compounds or extracts and health functional foods that have been approved for items in accordance with the laws of each country (the "Pharmaceuticals Act" in Korea) governing the manufacture and sale of '), and compounds or extracts whose functionality is recognized are included.

Another aspect of the present invention, fish collagen; and Centella asiatica, rosemary, rooibos, and a complex extract of camellia leaves; provides a method for preparing a health functional food for skin improvement, including.

Unless otherwise stated in the manufacturing method of the health functional food for skin improvement of the present invention, it is the same as described in the health functional food for skin improvement, and the description is omitted to avoid excessive repetition of the specification.

Since the health functional food composition for skin improvement of the present invention has excellent collagen synthesis promoting effect and tyrosinase inhibitory effect, it can be usefully used for skin improvement purposes such as skin wrinkles and/or elasticity improvement, and skin whitening.

In addition, since the health functional food for skin improvement of the present invention can improve the quality of the skin, it can be usefully used to make lively skin.

Hereinafter, the present invention will be described in more detail by way of Examples. However, these examples are for illustrative purposes only, and the scope of the present invention is not limited by these examples. In addition, it will be understood that terms not specifically defined herein have meanings commonly used in the technical field to which the present invention pertains.

Example

(1) Preparation of raw material complex extract

1) Pre-treatment step of raw material mixture

1-1) After thoroughly washing Centella asiatica (leaf), rosemary (leaf), rooibos (leaf) and camellia (leaf) (purchased at the oriental medicine market in Dongdaemun-gu or online open market), cut into 0.5 cm pieces 2: It was mixed evenly in a weight ratio of 2:1:1. Thereafter, purified water was added to 300 g of the mixture, and left for about 15 minutes to allow the purified water to be absorbed into the raw material, and the raw material mixture was filtered through a sieve to remove moisture.

1-2) The obtained raw material mixture is frozen at a very low temperature of about -40 to -50 ° C using a freezer (QF-700), and the frozen raw material mixture is taken out at room temperature and left for about 3 to 4 hours ( thaw), and the above process (freezing and thawing) was repeated 3 additional times (total 4 times).

1-3) The raw material mixture subjected to the above freezing and thawing steps was dried by hot air drying, and then frozen at an ultra-low temperature of about -40 to -50°C to complete the raw material mixture pretreatment step.

2) Preparation of raw material complex extract

2-1) 100 g of the pre-treated raw material mixture is pulverized to a size of about 400 to 500 μm through a grinder in a frozen state, 80% (w/v) ethanol of about 10 times the volume is added, and then stirred for 10 minutes to homogenize did

2-2) The homogenized raw material mixture was extracted while maintaining the temperature at 60 to 80° C. while continuously stirring for 3 hours using an ultrasonic extractor (Power sonica, Hwasin Tech).

Thereafter, the obtained extract was cooled to 4° C., left for about 5 hours, and filtered through a filter (6 μm) to separate the filtrate and residues, and the filtrate (first extract) was stored separately.

2-3) After adding about 10 times the volume of purified water to the separated residue, homogenization by stirring for 10 minutes, hot water extraction at about 130 to 150° C. for 3 hours, and cooling the obtained extract to 4° C., After leaving it for about 5 hours, it was filtered through a filter (6 μm) to separate the filtrate and the residue, and the filtrate (the second extract) was stored separately.

2-4) Then, the first and second extracts were mixed, concentrated under reduced pressure and freeze-dried (Eyela NE, Tokyo, Japan) to prepare a raw material complex extract.

(3) fish Blend of collagen and raw material complex extract

Commercially purchased low-molecular-weight fish collagen (Geltech, 500 to 1,000 Da) and the prepared raw material complex extract were mixed in a weight ratio of 2:1, which was used as a sample in Examples.

Comparative Example 1

In the pretreatment step of the raw material mixture of Example, a sample in which fish collagen and raw material complex extract were mixed was prepared in substantially the same manner as in Example, except that the freezing and thawing of steps 1-2 were repeated a total of two times. .

Comparative Example 2

In the preparation of the raw material complex extract of Examples, a sample in which fish collagen and the raw material complex extract were mixed was prepared in substantially the same manner as in Examples, except for extracting in the following manner.

3-1) 100 g of the raw material mixture pretreated in steps 1-1 to 1-3 of the above example is pulverized to a size of about 400 to 500 μm through a grinder in a frozen state, and after administering about 10 times the volume of purified water, It was homogenized by stirring for 10 minutes.

에서 3시간 동안 열수추출하고, 수득된 추출물을 4

로 냉각시키고, 약 5시간 동안 방치시킨 후, 필터(6 ㎛)로 여과하여 여과액 및 잔사물을 분리하고, 여과액(제1' 추출물)은 따로 보관하였다.3-2) about 130 to 150 of the homogenized raw material mixture

hot water extraction for 3 hours, and the obtained extract was

After cooling with a furnace and leaving it for about 5 hours, it was filtered through a filter (6 μm) to separate the filtrate and the residue, and the filtrate (the first 'extract) was stored separately.

3-3) After adding about 10 times the volume of 80% (w/v) ethanol to the separated residue, homogenize by stirring for 10 minutes, and ultrasonic extractor while maintaining the homogenized raw material mixture to be 60 to 80 ℃ (Power sonica, Hwashin Tech) was used for extraction while continuously stirring for 3 hours.

Thereafter, the obtained extract was cooled to 4° C., left for about 5 hours, filtered through a filter (6 μm) to separate the filtrate and residues, and the filtrate (2nd 'complex extract) was stored separately. did

3-4) Thereafter, the 1' and 2' extracts were mixed, concentrated under reduced pressure and freeze-dried (Eyela NE, Tokyo, Japan) to prepare a raw material complex extract.

Experimental Example 1. Confirmation of collagen synthesis enhancing effect

The dermal layer of the skin is composed of fibroblasts, collagen, and elastin organically, and wrinkles are generated as the organic structures such as collagen and elastin are destroyed by skin damage such as aging and ultraviolet rays. Procollagen is converted to collagen and increases the collagen content in the dermal layer, thereby improving or alleviating the skin.

Human-derived fibroblasts, WI-38 cells, were inoculated into a 24-well plate containing DMEM medium (2 x 10 ⁵ cells/well), and cultured for 24 hours at 37°C, 5% CO ₂ condition, and then in each well. After removing the medium, 1 mg/ml of each of Examples, Comparative Examples and Control (retinol) samples were treated, and then cultured again for 24 hours. Then, the cell medium was collected.

The amount of procollagen synthesis was measured using an ELISA kit (Procollagen Type I C-peptide EIA kit MK101, Takara Korea Biomedical Inc.), and the amount of procollagen production was calculated by measuring the absorbance at 450 nm.

[Formula 1]

Procollagen production rate (%) = (Procollagen production amount during sample treatment / Procollagen production amount of control group) x 100

sample procollagen generation rate ( % ) Example 145 Comparative Example 1 121 Comparative Example 2 127 control 100

As can be seen in Table 1, the Example exhibited a very excellent procollagen production promoting effect compared to the control known to promote procollagen synthesis.

In addition, in the case of Comparative Examples 1 and 2 with a difference in the production method, the procollagen production promoting effect was exhibited, but in the case of the Examples, a significant procollagen production promoting effect was also exhibited compared to Comparative Examples 1 and 2, so that the It was confirmed that the difference could also increase the effect of procollagen production.

The above results confirm that the composition of the present invention can effectively promote collagen production to improve wrinkles and elasticity of the skin caused by skin aging.

Experimental Example 2. Confirmation of inhibition of tyrosinase activity

In order to determine whether the composition of the present invention has tyrosinase inhibitory activity, the compositions of Examples and Comparative Examples were treated with B16F10 melanoma cell line, and the degree of inhibition of tyrosinase activity was measured.

Specifically, the B16F10 melanoma cell line was dispensed in a 6-well plate (1Х10 ⁵ /well), and the medium was removed after 24 hours. Thereafter, 1 mg/ml of arbutin was added to the samples of Examples and Comparative Examples and as a control, respectively, and cultured for 3 days. At this time, α-MSH, a melanin formation stimulator, was also treated. Then, washed twice with PBS, lysis buffer (100 mM sodium phosphate (pH 6.8), 0.1 mM PMSF, 1% Triton X-100) was added and dissolved at -80°C for 30 minutes. After the cells were collected from the culture vessel, the sodium hydroxide solution (1 N, 10% DMSO) was reacted at 65° C. for 1 hour to dissolve, and the amount of melanin produced by absorbance per unit cell number at 400 nm was measured, and the inhibition rate was It was calculated by the following formula and shown in Table 2.

[Formula 2]

Tyrosinase activity inhibition rate (%) = {(Melanin production in untreated group - Melanin production in test sample) / Melanin production in control group} X 100

sample tyrosinase activity inhibition ( % ) Example 82 Comparative Example 1 40 Comparative Example 2 69 arbutin 49 untreated group ^* -

( ^* means the group treated only with α-MSH)

As shown in Table 1, the cell line treated with Example showed a very high tyrosinase activity inhibition rate compared to the arbutin-treated group.

In addition, in the case of Comparative Examples 1 and 2 with a difference in the manufacturing method in the complex extract, the effect of inhibiting tyrosinase activity was shown, but the group treated with the Example showed a significant tyrosinase activity inhibitory effect in comparison with this. It was confirmed that the tyrosinase activity inhibitory effect can be significantly increased even by the difference of .

The above results confirm that the composition of the present invention can inhibit the production of pigments such as melanin by effectively inhibiting tyrosinase activity, thereby inhibiting the production of spots, etc. that may be generated during skin aging.

Claims (10)

fish collagen; and
In the health functional food composition for skin improvement comprising; Centella asiatica, rosemary, rooibos and a complex extract of camellia leaves,
The complex extract,
S1) Centella asiatica, rosemary, rooibos and camellia leaves are minced and mixed, purified water is added, left for 10 to 20 minutes, and then water is removed;
S2) freezing the water-removed mixture at -40 to -50°C, and then leaving it at room temperature for 3 to 4 hours;
S3) freezing the left mixture at -40 to -50°C after drying;
S4) pulverizing the raw material mixture in the frozen state, adding 80% (w/v) ethanol to the pulverized raw material mixture, and stirring to homogenize;
S5) sonicating the homogenized raw material mixture solution at 60 to 80° C. for 2 to 4 hours to perform ultrasonic extraction;
S6) separating the sonicated solution into a filtrate and a residue by filtering;
S7) adding purified water to the separated residue, followed by stirring to homogenize;
S8) heat-treating the homogenized solution at 130 to 150° C. for 2 to 4 hours to extract hot water;
S9) separating the heat-treated solution into a filtrate and a residue by filtering; and
S10) mixing the filtrate separated in steps S6 and S9;
The step S2 is a health functional food composition for skin improvement that is repeated 3 to 5 times. According to claim 1,
The fish collagen is a health functional food composition for skin improvement having a molecular weight of 500 to 1,000 Da. According to claim 1,
The complex extract is a health functional food composition for skin improvement that is extracted from a raw material mixture obtained by mixing Centella asiatica, rosemary, rooibos and camellia leaves in a weight ratio of 2:2:1:1. According to claim 1,
The fish collagen and complex extract are included in a weight ratio of 2: 1 health functional food composition for skin improvement. delete delete According to claim 1,
The pulverization of the step S4 is a health functional food composition for skin improvement that is performed to have a size of 400 to 500 μm. According to claim 1,
between steps S5 and S6; and cooling the extract obtained between steps S8 and S9 to 2 to 6°C, and leaving it to stand for 4 to 6 hours. delete delete