TWI534265B - 用於操縱植物之光合細胞中果聚糖生合成之方法、基因建構體及基因轉殖植物細胞 - Google Patents
用於操縱植物之光合細胞中果聚糖生合成之方法、基因建構體及基因轉殖植物細胞 Download PDFInfo
- Publication number
- TWI534265B TWI534265B TW098130928A TW98130928A TWI534265B TW I534265 B TWI534265 B TW I534265B TW 098130928 A TW098130928 A TW 098130928A TW 98130928 A TW98130928 A TW 98130928A TW I534265 B TWI534265 B TW I534265B
- Authority
- TW
- Taiwan
- Prior art keywords
- gene
- fusion
- plant
- fft
- sst
- Prior art date
Links
- 229920002670 Fructan Polymers 0.000 title claims description 185
- 238000000034 method Methods 0.000 title claims description 76
- 210000004027 cell Anatomy 0.000 title claims description 66
- 230000002068 genetic effect Effects 0.000 title claims description 54
- 230000015572 biosynthetic process Effects 0.000 title claims description 52
- 210000001916 photosynthetic cell Anatomy 0.000 title claims description 37
- 230000009261 transgenic effect Effects 0.000 title description 76
- 230000004927 fusion Effects 0.000 claims description 327
- 108090000623 proteins and genes Proteins 0.000 claims description 317
- 239000012634 fragment Substances 0.000 claims description 104
- 150000007523 nucleic acids Chemical class 0.000 claims description 82
- 102000039446 nucleic acids Human genes 0.000 claims description 70
- 108020004707 nucleic acids Proteins 0.000 claims description 70
- 230000001965 increasing effect Effects 0.000 claims description 64
- 238000012546 transfer Methods 0.000 claims description 62
- 102000004190 Enzymes Human genes 0.000 claims description 59
- 108090000790 Enzymes Proteins 0.000 claims description 59
- 239000002028 Biomass Substances 0.000 claims description 54
- 108020001507 fusion proteins Proteins 0.000 claims description 42
- 102000037865 fusion proteins Human genes 0.000 claims description 42
- 101001074840 Asparagus officinalis 6(G)-fructosyltransferase Proteins 0.000 claims description 33
- 101001074841 Allium cepa Bifunctional 6(G)-fructosyltransferase/2,1-fructan:2,1-fructan 1-fructosyltransferase Proteins 0.000 claims description 30
- 230000032683 aging Effects 0.000 claims description 27
- 230000001851 biosynthetic effect Effects 0.000 claims description 27
- 150000001720 carbohydrates Chemical class 0.000 claims description 22
- 101100534435 Festuca arundinacea 1-SST gene Proteins 0.000 claims description 19
- 238000003786 synthesis reaction Methods 0.000 claims description 19
- 101150078498 MYB gene Proteins 0.000 claims description 14
- 230000006696 biosynthetic metabolic pathway Effects 0.000 claims description 10
- UQHKFADEQIVWID-UHFFFAOYSA-N cytokinin Natural products C1=NC=2C(NCC=C(CO)C)=NC=NC=2N1C1CC(O)C(CO)O1 UQHKFADEQIVWID-UHFFFAOYSA-N 0.000 claims description 7
- 239000004062 cytokinin Substances 0.000 claims description 7
- 108010042889 Inulosucrase Proteins 0.000 description 392
- 241000196324 Embryophyta Species 0.000 description 382
- 240000004296 Lolium perenne Species 0.000 description 125
- 230000014509 gene expression Effects 0.000 description 120
- 239000013598 vector Substances 0.000 description 90
- 210000001519 tissue Anatomy 0.000 description 64
- 230000001105 regulatory effect Effects 0.000 description 58
- 241000209140 Triticum Species 0.000 description 57
- 230000012010 growth Effects 0.000 description 57
- 239000002773 nucleotide Substances 0.000 description 55
- 125000003729 nucleotide group Chemical group 0.000 description 55
- 230000009466 transformation Effects 0.000 description 47
- 102000004169 proteins and genes Human genes 0.000 description 44
- 229930006000 Sucrose Natural products 0.000 description 37
- 239000005720 sucrose Substances 0.000 description 37
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 36
- 210000002706 plastid Anatomy 0.000 description 35
- 230000000243 photosynthetic effect Effects 0.000 description 34
- 241000234643 Festuca arundinacea Species 0.000 description 33
- 108020004705 Codon Proteins 0.000 description 32
- 241000209082 Lolium Species 0.000 description 30
- 125000003275 alpha amino acid group Chemical group 0.000 description 30
- 238000012216 screening Methods 0.000 description 30
- 235000021307 Triticum Nutrition 0.000 description 27
- 238000003752 polymerase chain reaction Methods 0.000 description 27
- ZFTFOHBYVDOAMH-XNOIKFDKSA-N (2r,3s,4s,5r)-5-[[(2r,3s,4s,5r)-5-[[(2r,3s,4s,5r)-3,4-dihydroxy-2,5-bis(hydroxymethyl)oxolan-2-yl]oxymethyl]-3,4-dihydroxy-2-(hydroxymethyl)oxolan-2-yl]oxymethyl]-2-(hydroxymethyl)oxolane-2,3,4-triol Chemical class O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)OC[C@@H]1[C@@H](O)[C@H](O)[C@](CO)(OC[C@@H]2[C@H]([C@H](O)[C@@](O)(CO)O2)O)O1 ZFTFOHBYVDOAMH-XNOIKFDKSA-N 0.000 description 26
- 241000219194 Arabidopsis Species 0.000 description 26
- 108020004414 DNA Proteins 0.000 description 26
- 150000001413 amino acids Chemical class 0.000 description 24
- 238000004458 analytical method Methods 0.000 description 24
- 230000035876 healing Effects 0.000 description 24
- 239000003550 marker Substances 0.000 description 24
- 238000012360 testing method Methods 0.000 description 24
- 238000005516 engineering process Methods 0.000 description 21
- 239000004459 forage Substances 0.000 description 20
- 244000042324 Trifolium repens Species 0.000 description 19
- 239000002299 complementary DNA Substances 0.000 description 19
- 235000014633 carbohydrates Nutrition 0.000 description 18
- 238000004519 manufacturing process Methods 0.000 description 18
- 239000000047 product Substances 0.000 description 17
- 241000589158 Agrobacterium Species 0.000 description 16
- 230000008238 biochemical pathway Effects 0.000 description 16
- 239000005715 Fructose Substances 0.000 description 15
- 108700007698 Genetic Terminator Regions Proteins 0.000 description 15
- 230000037361 pathway Effects 0.000 description 15
- 230000008929 regeneration Effects 0.000 description 15
- 238000011069 regeneration method Methods 0.000 description 15
- 241000234642 Festuca Species 0.000 description 14
- 238000012742 biochemical analysis Methods 0.000 description 14
- 239000000203 mixture Substances 0.000 description 14
- 229920001184 polypeptide Polymers 0.000 description 14
- 108090000765 processed proteins & peptides Proteins 0.000 description 14
- 102000004196 processed proteins & peptides Human genes 0.000 description 14
- 108091008146 restriction endonucleases Proteins 0.000 description 14
- 241000701489 Cauliflower mosaic virus Species 0.000 description 13
- 229930091371 Fructose Natural products 0.000 description 13
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 13
- 108010051210 beta-Fructofuranosidase Proteins 0.000 description 13
- 230000001976 improved effect Effects 0.000 description 13
- 235000011073 invertase Nutrition 0.000 description 13
- 239000002245 particle Substances 0.000 description 13
- 230000000717 retained effect Effects 0.000 description 13
- 238000006467 substitution reaction Methods 0.000 description 13
- 101000981789 Arabidopsis thaliana Transcription factor MYB32 Proteins 0.000 description 12
- 108010060309 Glucuronidase Proteins 0.000 description 12
- 102000053187 Glucuronidase Human genes 0.000 description 12
- 235000013540 Trifolium repens var repens Nutrition 0.000 description 12
- 238000010586 diagram Methods 0.000 description 12
- 230000006870 function Effects 0.000 description 12
- 101150029559 hph gene Proteins 0.000 description 12
- 230000001404 mediated effect Effects 0.000 description 12
- 230000008901 benefit Effects 0.000 description 11
- 239000011159 matrix material Substances 0.000 description 11
- 239000002689 soil Substances 0.000 description 11
- 240000000385 Brassica napus var. napus Species 0.000 description 10
- 108091028043 Nucleic acid sequence Proteins 0.000 description 10
- 238000012512 characterization method Methods 0.000 description 10
- 230000000694 effects Effects 0.000 description 10
- 241001233957 eudicotyledons Species 0.000 description 10
- 238000002474 experimental method Methods 0.000 description 10
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 10
- 239000010931 gold Substances 0.000 description 10
- 229910052737 gold Inorganic materials 0.000 description 10
- 239000001573 invertase Substances 0.000 description 10
- 235000014698 Brassica juncea var multisecta Nutrition 0.000 description 9
- 235000006008 Brassica napus var napus Nutrition 0.000 description 9
- 235000006618 Brassica rapa subsp oleifera Nutrition 0.000 description 9
- 235000004977 Brassica sinapistrum Nutrition 0.000 description 9
- 244000061176 Nicotiana tabacum Species 0.000 description 9
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 9
- 108700019146 Transgenes Proteins 0.000 description 9
- 230000003115 biocidal effect Effects 0.000 description 9
- 239000000543 intermediate Substances 0.000 description 9
- 230000006798 recombination Effects 0.000 description 9
- 238000005215 recombination Methods 0.000 description 9
- 230000014616 translation Effects 0.000 description 9
- 244000025254 Cannabis sativa Species 0.000 description 8
- 108010003581 Ribulose-bisphosphate carboxylase Proteins 0.000 description 8
- 230000003111 delayed effect Effects 0.000 description 8
- 238000000926 separation method Methods 0.000 description 8
- 238000013519 translation Methods 0.000 description 8
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 7
- 238000009825 accumulation Methods 0.000 description 7
- 230000000408 embryogenic effect Effects 0.000 description 7
- 239000013604 expression vector Substances 0.000 description 7
- 230000002441 reversible effect Effects 0.000 description 7
- 238000000844 transformation Methods 0.000 description 7
- 102000004157 Hydrolases Human genes 0.000 description 6
- 108090000604 Hydrolases Proteins 0.000 description 6
- 240000003829 Sorghum propinquum Species 0.000 description 6
- 235000011684 Sorghum saccharatum Nutrition 0.000 description 6
- 238000007792 addition Methods 0.000 description 6
- 238000005571 anion exchange chromatography Methods 0.000 description 6
- 235000013339 cereals Nutrition 0.000 description 6
- 230000008859 change Effects 0.000 description 6
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 6
- 230000005714 functional activity Effects 0.000 description 6
- 230000007062 hydrolysis Effects 0.000 description 6
- 238000006460 hydrolysis reaction Methods 0.000 description 6
- 238000000338 in vitro Methods 0.000 description 6
- 238000002347 injection Methods 0.000 description 6
- 239000007924 injection Substances 0.000 description 6
- 230000014759 maintenance of location Effects 0.000 description 6
- 238000009304 pastoral farming Methods 0.000 description 6
- 210000001938 protoplast Anatomy 0.000 description 6
- 230000002786 root growth Effects 0.000 description 6
- 230000002103 transcriptional effect Effects 0.000 description 6
- -1 turf Substances 0.000 description 6
- 229920001817 Agar Polymers 0.000 description 5
- 241000589155 Agrobacterium tumefaciens Species 0.000 description 5
- 108010054576 Deoxyribonuclease EcoRI Proteins 0.000 description 5
- 102000005744 Glycoside Hydrolases Human genes 0.000 description 5
- 108010031186 Glycoside Hydrolases Proteins 0.000 description 5
- 244000100545 Lolium multiflorum Species 0.000 description 5
- 241001465754 Metazoa Species 0.000 description 5
- 108090000854 Oxidoreductases Proteins 0.000 description 5
- 102000004316 Oxidoreductases Human genes 0.000 description 5
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 5
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 5
- 240000008042 Zea mays Species 0.000 description 5
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 5
- 230000033228 biological regulation Effects 0.000 description 5
- 210000003763 chloroplast Anatomy 0.000 description 5
- 230000001186 cumulative effect Effects 0.000 description 5
- 235000021186 dishes Nutrition 0.000 description 5
- 210000002257 embryonic structure Anatomy 0.000 description 5
- 101150012864 ipt gene Proteins 0.000 description 5
- 239000002609 medium Substances 0.000 description 5
- 230000004048 modification Effects 0.000 description 5
- 238000012986 modification Methods 0.000 description 5
- 210000000056 organ Anatomy 0.000 description 5
- 230000008569 process Effects 0.000 description 5
- 230000001052 transient effect Effects 0.000 description 5
- 101150090724 3 gene Proteins 0.000 description 4
- 101001076701 Arabidopsis thaliana Beta-fructofuranosidase, insoluble isoenzyme CWINV3 Proteins 0.000 description 4
- 102000014914 Carrier Proteins Human genes 0.000 description 4
- ZAQJHHRNXZUBTE-NQXXGFSBSA-N D-ribulose Chemical compound OC[C@@H](O)[C@@H](O)C(=O)CO ZAQJHHRNXZUBTE-NQXXGFSBSA-N 0.000 description 4
- ZAQJHHRNXZUBTE-UHFFFAOYSA-N D-threo-2-Pentulose Natural products OCC(O)C(O)C(=O)CO ZAQJHHRNXZUBTE-UHFFFAOYSA-N 0.000 description 4
- 241000209510 Liliopsida Species 0.000 description 4
- 241000219823 Medicago Species 0.000 description 4
- 235000017587 Medicago sativa ssp. sativa Nutrition 0.000 description 4
- 241000209504 Poaceae Species 0.000 description 4
- 101000702488 Rattus norvegicus High affinity cationic amino acid transporter 1 Proteins 0.000 description 4
- 101710181960 Sucrose:sucrose 1-fructosyltransferase Proteins 0.000 description 4
- 241000219793 Trifolium Species 0.000 description 4
- 244000098338 Triticum aestivum Species 0.000 description 4
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 4
- 230000006978 adaptation Effects 0.000 description 4
- 239000008272 agar Substances 0.000 description 4
- 238000000246 agarose gel electrophoresis Methods 0.000 description 4
- 108091008324 binding proteins Proteins 0.000 description 4
- 239000003795 chemical substances by application Substances 0.000 description 4
- 229930002868 chlorophyll a Natural products 0.000 description 4
- 229930002869 chlorophyll b Natural products 0.000 description 4
- NSMUHPMZFPKNMZ-VBYMZDBQSA-M chlorophyll b Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C=O)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 NSMUHPMZFPKNMZ-VBYMZDBQSA-M 0.000 description 4
- 238000010276 construction Methods 0.000 description 4
- 235000005822 corn Nutrition 0.000 description 4
- 238000012217 deletion Methods 0.000 description 4
- 230000037430 deletion Effects 0.000 description 4
- 101150054603 ft gene Proteins 0.000 description 4
- 238000011534 incubation Methods 0.000 description 4
- 230000006698 induction Effects 0.000 description 4
- 230000008595 infiltration Effects 0.000 description 4
- 238000001764 infiltration Methods 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 238000007479 molecular analysis Methods 0.000 description 4
- 235000016709 nutrition Nutrition 0.000 description 4
- 230000029553 photosynthesis Effects 0.000 description 4
- 238000010672 photosynthesis Methods 0.000 description 4
- 230000035755 proliferation Effects 0.000 description 4
- 230000001737 promoting effect Effects 0.000 description 4
- 238000011002 quantification Methods 0.000 description 4
- 230000002829 reductive effect Effects 0.000 description 4
- 238000003757 reverse transcription PCR Methods 0.000 description 4
- 210000004767 rumen Anatomy 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 239000003104 tissue culture media Substances 0.000 description 4
- 238000010361 transduction Methods 0.000 description 4
- 230000026683 transduction Effects 0.000 description 4
- 230000010474 transient expression Effects 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 244000291564 Allium cepa Species 0.000 description 3
- 235000002732 Allium cepa var. cepa Nutrition 0.000 description 3
- 241000219195 Arabidopsis thaliana Species 0.000 description 3
- 101000981772 Arabidopsis thaliana Transcription factor MYB3 Proteins 0.000 description 3
- 235000011331 Brassica Nutrition 0.000 description 3
- 241000219198 Brassica Species 0.000 description 3
- 241000218631 Coniferophyta Species 0.000 description 3
- YQYJSBFKSSDGFO-UHFFFAOYSA-N Epihygromycin Natural products OC1C(O)C(C(=O)C)OC1OC(C(=C1)O)=CC=C1C=C(C)C(=O)NC1C(O)C(O)C2OCOC2C1O YQYJSBFKSSDGFO-UHFFFAOYSA-N 0.000 description 3
- 235000010469 Glycine max Nutrition 0.000 description 3
- 235000007340 Hordeum vulgare Nutrition 0.000 description 3
- 240000005979 Hordeum vulgare Species 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 244000183278 Nephelium litchi Species 0.000 description 3
- 240000007594 Oryza sativa Species 0.000 description 3
- 235000007164 Oryza sativa Nutrition 0.000 description 3
- 241001268782 Paspalum dilatatum Species 0.000 description 3
- 241000282849 Ruminantia Species 0.000 description 3
- 240000000111 Saccharum officinarum Species 0.000 description 3
- 235000007201 Saccharum officinarum Nutrition 0.000 description 3
- 235000007238 Secale cereale Nutrition 0.000 description 3
- 108090000848 Ubiquitin Proteins 0.000 description 3
- 102000044159 Ubiquitin Human genes 0.000 description 3
- 230000036579 abiotic stress Effects 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- 238000010367 cloning Methods 0.000 description 3
- 238000005520 cutting process Methods 0.000 description 3
- 238000009795 derivation Methods 0.000 description 3
- 230000029087 digestion Effects 0.000 description 3
- 230000024346 drought recovery Effects 0.000 description 3
- 230000009977 dual effect Effects 0.000 description 3
- 235000013399 edible fruits Nutrition 0.000 description 3
- 238000010195 expression analysis Methods 0.000 description 3
- 238000010230 functional analysis Methods 0.000 description 3
- 238000009396 hybridization Methods 0.000 description 3
- 108010005131 levanase Proteins 0.000 description 3
- 244000144972 livestock Species 0.000 description 3
- 108020004999 messenger RNA Proteins 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- 230000035764 nutrition Effects 0.000 description 3
- 230000001691 photoregulatory effect Effects 0.000 description 3
- 239000013612 plasmid Substances 0.000 description 3
- 238000006116 polymerization reaction Methods 0.000 description 3
- 230000002062 proliferating effect Effects 0.000 description 3
- 238000003762 quantitative reverse transcription PCR Methods 0.000 description 3
- 230000001850 reproductive effect Effects 0.000 description 3
- 235000009566 rice Nutrition 0.000 description 3
- 210000002107 sheath cell Anatomy 0.000 description 3
- 241000894007 species Species 0.000 description 3
- 230000035882 stress Effects 0.000 description 3
- 230000002194 synthesizing effect Effects 0.000 description 3
- 230000002792 vascular Effects 0.000 description 3
- IAJOBQBIJHVGMQ-UHFFFAOYSA-N 2-amino-4-[hydroxy(methyl)phosphoryl]butanoic acid Chemical compound CP(O)(=O)CCC(N)C(O)=O IAJOBQBIJHVGMQ-UHFFFAOYSA-N 0.000 description 2
- 244000205574 Acorus calamus Species 0.000 description 2
- 235000006480 Acorus calamus Nutrition 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 235000007319 Avena orientalis Nutrition 0.000 description 2
- 244000075850 Avena orientalis Species 0.000 description 2
- 240000002791 Brassica napus Species 0.000 description 2
- 235000011293 Brassica napus Nutrition 0.000 description 2
- 108091035707 Consensus sequence Proteins 0.000 description 2
- 229920000742 Cotton Polymers 0.000 description 2
- 244000077283 Distichlis palmeri Species 0.000 description 2
- 235000005422 Distichlis palmeri Nutrition 0.000 description 2
- 241000380130 Ehrharta erecta Species 0.000 description 2
- 239000005561 Glufosinate Substances 0.000 description 2
- 244000068988 Glycine max Species 0.000 description 2
- 244000299507 Gossypium hirsutum Species 0.000 description 2
- 229920001202 Inulin Polymers 0.000 description 2
- 101100288095 Klebsiella pneumoniae neo gene Proteins 0.000 description 2
- HGCNKOLVKRAVHD-UHFFFAOYSA-N L-Met-L-Phe Natural products CSCCC(N)C(=O)NC(C(O)=O)CC1=CC=CC=C1 HGCNKOLVKRAVHD-UHFFFAOYSA-N 0.000 description 2
- 235000003228 Lactuca sativa Nutrition 0.000 description 2
- 240000008415 Lactuca sativa Species 0.000 description 2
- 101150017476 MYB32 gene Proteins 0.000 description 2
- HGCNKOLVKRAVHD-RYUDHWBXSA-N Met-Phe Chemical compound CSCC[C@H](N)C(=O)N[C@H](C(O)=O)CC1=CC=CC=C1 HGCNKOLVKRAVHD-RYUDHWBXSA-N 0.000 description 2
- 240000003433 Miscanthus floridulus Species 0.000 description 2
- 241000207746 Nicotiana benthamiana Species 0.000 description 2
- 241001520808 Panicum virgatum Species 0.000 description 2
- 240000007377 Petunia x hybrida Species 0.000 description 2
- XYFCBTPGUUZFHI-UHFFFAOYSA-N Phosphine Chemical compound P XYFCBTPGUUZFHI-UHFFFAOYSA-N 0.000 description 2
- 108700001094 Plant Genes Proteins 0.000 description 2
- 241000209056 Secale Species 0.000 description 2
- 235000002595 Solanum tuberosum Nutrition 0.000 description 2
- 244000061456 Solanum tuberosum Species 0.000 description 2
- 235000009337 Spinacia oleracea Nutrition 0.000 description 2
- 244000300264 Spinacia oleracea Species 0.000 description 2
- 102000004357 Transferases Human genes 0.000 description 2
- 108090000992 Transferases Proteins 0.000 description 2
- 235000015724 Trifolium pratense Nutrition 0.000 description 2
- 235000010729 Trifolium repens Nutrition 0.000 description 2
- 240000002805 Triticum turgidum Species 0.000 description 2
- 244000274883 Urtica dioica Species 0.000 description 2
- 235000009108 Urtica dioica Nutrition 0.000 description 2
- 241000700605 Viruses Species 0.000 description 2
- 239000000370 acceptor Substances 0.000 description 2
- OIRDTQYFTABQOQ-KQYNXXCUSA-N adenosine Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O OIRDTQYFTABQOQ-KQYNXXCUSA-N 0.000 description 2
- 230000003698 anagen phase Effects 0.000 description 2
- 101150010487 are gene Proteins 0.000 description 2
- 210000004436 artificial bacterial chromosome Anatomy 0.000 description 2
- 125000003118 aryl group Chemical group 0.000 description 2
- 229930002875 chlorophyll Natural products 0.000 description 2
- 235000019804 chlorophyll Nutrition 0.000 description 2
- 230000002759 chromosomal effect Effects 0.000 description 2
- 238000003776 cleavage reaction Methods 0.000 description 2
- 230000004186 co-expression Effects 0.000 description 2
- 238000005094 computer simulation Methods 0.000 description 2
- 239000001177 diphosphate Substances 0.000 description 2
- 238000004520 electroporation Methods 0.000 description 2
- BJHIKXHVCXFQLS-UYFOZJQFSA-N fructose group Chemical group OCC(=O)[C@@H](O)[C@H](O)[C@H](O)CO BJHIKXHVCXFQLS-UYFOZJQFSA-N 0.000 description 2
- 238000010363 gene targeting Methods 0.000 description 2
- 150000004676 glycans Chemical class 0.000 description 2
- 125000003630 glycyl group Chemical group [H]N([H])C([H])([H])C(*)=O 0.000 description 2
- 238000011065 in-situ storage Methods 0.000 description 2
- 238000010348 incorporation Methods 0.000 description 2
- 230000001939 inductive effect Effects 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- 230000003993 interaction Effects 0.000 description 2
- JYJIGFIDKWBXDU-MNNPPOADSA-N inulin Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)OC[C@]1(OC[C@]2(OC[C@]3(OC[C@]4(OC[C@]5(OC[C@]6(OC[C@]7(OC[C@]8(OC[C@]9(OC[C@]%10(OC[C@]%11(OC[C@]%12(OC[C@]%13(OC[C@]%14(OC[C@]%15(OC[C@]%16(OC[C@]%17(OC[C@]%18(OC[C@]%19(OC[C@]%20(OC[C@]%21(OC[C@]%22(OC[C@]%23(OC[C@]%24(OC[C@]%25(OC[C@]%26(OC[C@]%27(OC[C@]%28(OC[C@]%29(OC[C@]%30(OC[C@]%31(OC[C@]%32(OC[C@]%33(OC[C@]%34(OC[C@]%35(OC[C@]%36(O[C@@H]%37[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O%37)O)[C@H]([C@H](O)[C@@H](CO)O%36)O)[C@H]([C@H](O)[C@@H](CO)O%35)O)[C@H]([C@H](O)[C@@H](CO)O%34)O)[C@H]([C@H](O)[C@@H](CO)O%33)O)[C@H]([C@H](O)[C@@H](CO)O%32)O)[C@H]([C@H](O)[C@@H](CO)O%31)O)[C@H]([C@H](O)[C@@H](CO)O%30)O)[C@H]([C@H](O)[C@@H](CO)O%29)O)[C@H]([C@H](O)[C@@H](CO)O%28)O)[C@H]([C@H](O)[C@@H](CO)O%27)O)[C@H]([C@H](O)[C@@H](CO)O%26)O)[C@H]([C@H](O)[C@@H](CO)O%25)O)[C@H]([C@H](O)[C@@H](CO)O%24)O)[C@H]([C@H](O)[C@@H](CO)O%23)O)[C@H]([C@H](O)[C@@H](CO)O%22)O)[C@H]([C@H](O)[C@@H](CO)O%21)O)[C@H]([C@H](O)[C@@H](CO)O%20)O)[C@H]([C@H](O)[C@@H](CO)O%19)O)[C@H]([C@H](O)[C@@H](CO)O%18)O)[C@H]([C@H](O)[C@@H](CO)O%17)O)[C@H]([C@H](O)[C@@H](CO)O%16)O)[C@H]([C@H](O)[C@@H](CO)O%15)O)[C@H]([C@H](O)[C@@H](CO)O%14)O)[C@H]([C@H](O)[C@@H](CO)O%13)O)[C@H]([C@H](O)[C@@H](CO)O%12)O)[C@H]([C@H](O)[C@@H](CO)O%11)O)[C@H]([C@H](O)[C@@H](CO)O%10)O)[C@H]([C@H](O)[C@@H](CO)O9)O)[C@H]([C@H](O)[C@@H](CO)O8)O)[C@H]([C@H](O)[C@@H](CO)O7)O)[C@H]([C@H](O)[C@@H](CO)O6)O)[C@H]([C@H](O)[C@@H](CO)O5)O)[C@H]([C@H](O)[C@@H](CO)O4)O)[C@H]([C@H](O)[C@@H](CO)O3)O)[C@H]([C@H](O)[C@@H](CO)O2)O)[C@@H](O)[C@H](O)[C@@H](CO)O1 JYJIGFIDKWBXDU-MNNPPOADSA-N 0.000 description 2
- 229940029339 inulin Drugs 0.000 description 2
- 238000002955 isolation Methods 0.000 description 2
- 230000002015 leaf growth Effects 0.000 description 2
- 210000001161 mammalian embryo Anatomy 0.000 description 2
- 235000013372 meat Nutrition 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 230000002503 metabolic effect Effects 0.000 description 2
- 108010068488 methionylphenylalanine Proteins 0.000 description 2
- 239000013642 negative control Substances 0.000 description 2
- 230000008520 organization Effects 0.000 description 2
- 230000002018 overexpression Effects 0.000 description 2
- 230000037039 plant physiology Effects 0.000 description 2
- 238000002264 polyacrylamide gel electrophoresis Methods 0.000 description 2
- 230000004853 protein function Effects 0.000 description 2
- 235000013526 red clover Nutrition 0.000 description 2
- 239000012882 rooting medium Substances 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 230000007017 scission Effects 0.000 description 2
- 238000010187 selection method Methods 0.000 description 2
- 238000009331 sowing Methods 0.000 description 2
- 239000010902 straw Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- 230000002195 synergetic effect Effects 0.000 description 2
- 238000004809 thin layer chromatography Methods 0.000 description 2
- 238000013518 transcription Methods 0.000 description 2
- 230000035897 transcription Effects 0.000 description 2
- 238000001890 transfection Methods 0.000 description 2
- 230000001131 transforming effect Effects 0.000 description 2
- 108091005703 transmembrane proteins Proteins 0.000 description 2
- 102000035160 transmembrane proteins Human genes 0.000 description 2
- 150000003641 trioses Chemical class 0.000 description 2
- 150000004043 trisaccharides Chemical class 0.000 description 2
- 238000011144 upstream manufacturing Methods 0.000 description 2
- 230000003612 virological effect Effects 0.000 description 2
- 238000003809 water extraction Methods 0.000 description 2
- 102000004899 14-3-3 Proteins Human genes 0.000 description 1
- 101710112812 14-3-3 protein Proteins 0.000 description 1
- 239000005631 2,4-Dichlorophenoxyacetic acid Substances 0.000 description 1
- 101150033839 4 gene Proteins 0.000 description 1
- 101710145411 Acid beta-fructofuranosidase Proteins 0.000 description 1
- 241001327399 Andropogon gerardii Species 0.000 description 1
- 241000490494 Arabis Species 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 240000000724 Berberis vulgaris Species 0.000 description 1
- 239000002126 C01EB10 - Adenosine Substances 0.000 description 1
- 241000287937 Colinus Species 0.000 description 1
- 241000195493 Cryptophyta Species 0.000 description 1
- 241000192700 Cyanobacteria Species 0.000 description 1
- 108010066133 D-octopine dehydrogenase Proteins 0.000 description 1
- YAHZABJORDUQGO-NQXXGFSBSA-N D-ribulose 1,5-bisphosphate Chemical compound OP(=O)(O)OC[C@@H](O)[C@@H](O)C(=O)COP(O)(O)=O YAHZABJORDUQGO-NQXXGFSBSA-N 0.000 description 1
- 238000001712 DNA sequencing Methods 0.000 description 1
- 108010006731 Dimethylallyltranstransferase Proteins 0.000 description 1
- 102000005454 Dimethylallyltranstransferase Human genes 0.000 description 1
- 108010042407 Endonucleases Proteins 0.000 description 1
- 102000004533 Endonucleases Human genes 0.000 description 1
- 101100437498 Escherichia coli (strain K12) uidA gene Proteins 0.000 description 1
- 108700023372 Glycosyltransferases Proteins 0.000 description 1
- 102000051366 Glycosyltransferases Human genes 0.000 description 1
- 108091092195 Intron Proteins 0.000 description 1
- 108010025815 Kanamycin Kinase Proteins 0.000 description 1
- FAIXYKHYOGVFKA-UHFFFAOYSA-N Kinetin Natural products N=1C=NC=2N=CNC=2C=1N(C)C1=CC=CO1 FAIXYKHYOGVFKA-UHFFFAOYSA-N 0.000 description 1
- 241000235058 Komagataella pastoris Species 0.000 description 1
- 241001480167 Lotus japonicus Species 0.000 description 1
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 1
- 240000003183 Manihot esculenta Species 0.000 description 1
- 235000016735 Manihot esculenta subsp esculenta Nutrition 0.000 description 1
- 108010052285 Membrane Proteins Proteins 0.000 description 1
- IAOZJIPTCAWIRG-UHFFFAOYSA-N Methyl alpha-aspartylphenylalaninate Chemical compound OC(=O)CC(N)C(=O)NC(C(=O)OC)CC1=CC=CC=C1 IAOZJIPTCAWIRG-UHFFFAOYSA-N 0.000 description 1
- 108700011259 MicroRNAs Proteins 0.000 description 1
- 241001434359 Muhlenbergia phleoides Species 0.000 description 1
- 108700005084 Multigene Family Proteins 0.000 description 1
- 240000002853 Nelumbo nucifera Species 0.000 description 1
- 235000006508 Nelumbo nucifera Nutrition 0.000 description 1
- 235000006510 Nelumbo pentapetala Nutrition 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 238000000636 Northern blotting Methods 0.000 description 1
- 241000238633 Odonata Species 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 241000209046 Pennisetum Species 0.000 description 1
- 239000002202 Polyethylene glycol Substances 0.000 description 1
- 108010076504 Protein Sorting Signals Proteins 0.000 description 1
- 238000011529 RT qPCR Methods 0.000 description 1
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 description 1
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 description 1
- 108700008625 Reporter Genes Proteins 0.000 description 1
- 241000589180 Rhizobium Species 0.000 description 1
- 240000003768 Solanum lycopersicum Species 0.000 description 1
- 238000002105 Southern blotting Methods 0.000 description 1
- 101710195626 Transcriptional activator protein Proteins 0.000 description 1
- 241000218989 Trichosanthes Species 0.000 description 1
- 235000007264 Triticum durum Nutrition 0.000 description 1
- 241000209143 Triticum turgidum subsp. durum Species 0.000 description 1
- 244000126014 Valeriana officinalis Species 0.000 description 1
- 235000013832 Valeriana officinalis Nutrition 0.000 description 1
- IXKSXJFAGXLQOQ-XISFHERQSA-N WHWLQLKPGQPMY Chemical group C([C@@H](C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(C)C)C(=O)N1CCC[C@H]1C(=O)NCC(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(O)=O)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(O)=O)NC(=O)[C@@H](N)CC=1C2=CC=CC=C2NC=1)C1=CNC=N1 IXKSXJFAGXLQOQ-XISFHERQSA-N 0.000 description 1
- 101500015412 Zea mays Ubiquitin Proteins 0.000 description 1
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 108020002494 acetyltransferase Proteins 0.000 description 1
- 102000005421 acetyltransferase Human genes 0.000 description 1
- 229960005305 adenosine Drugs 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 125000000539 amino acid group Chemical group 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 238000005349 anion exchange Methods 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 210000001106 artificial yeast chromosome Anatomy 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 238000004166 bioassay Methods 0.000 description 1
- 239000002551 biofuel Substances 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 238000005422 blasting Methods 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 230000003197 catalytic effect Effects 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 230000034994 death Effects 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000001212 derivatisation Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 235000019621 digestibility Nutrition 0.000 description 1
- 239000003623 enhancer Substances 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 230000001747 exhibiting effect Effects 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 230000001744 histochemical effect Effects 0.000 description 1
- 108010002685 hygromycin-B kinase Proteins 0.000 description 1
- 238000000126 in silico method Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 125000001972 isopentyl group Chemical group [H]C([H])([H])C([H])(C([H])([H])[H])C([H])([H])C([H])([H])* 0.000 description 1
- 125000000555 isopropenyl group Chemical group [H]\C([H])=C(\*)C([H])([H])[H] 0.000 description 1
- QANMHLXAZMSUEX-UHFFFAOYSA-N kinetin Chemical compound N=1C=NC=2N=CNC=2C=1NCC1=CC=CO1 QANMHLXAZMSUEX-UHFFFAOYSA-N 0.000 description 1
- 229960001669 kinetin Drugs 0.000 description 1
- AIHDCSAXVMAMJH-GFBKWZILSA-N levan Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)OC[C@@H]1[C@@H](O)[C@H](O)[C@](CO)(CO[C@@H]2[C@H]([C@H](O)[C@@](O)(CO)O2)O)O1 AIHDCSAXVMAMJH-GFBKWZILSA-N 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 235000009973 maize Nutrition 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000000442 meristematic effect Effects 0.000 description 1
- 210000000473 mesophyll cell Anatomy 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 239000002679 microRNA Substances 0.000 description 1
- 238000000520 microinjection Methods 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 230000004899 motility Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 108010058731 nopaline synthase Proteins 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 238000007500 overflow downdraw method Methods 0.000 description 1
- 230000036961 partial effect Effects 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 230000002688 persistence Effects 0.000 description 1
- 229910000073 phosphorus hydride Inorganic materials 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 230000008488 polyadenylation Effects 0.000 description 1
- 229920001223 polyethylene glycol Polymers 0.000 description 1
- 239000013641 positive control Substances 0.000 description 1
- 230000004481 post-translational protein modification Effects 0.000 description 1
- 230000003389 potentiating effect Effects 0.000 description 1
- 238000004382 potting Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 238000001273 protein sequence alignment Methods 0.000 description 1
- 230000009257 reactivity Effects 0.000 description 1
- 238000010188 recombinant method Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000010076 replication Effects 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 210000003705 ribosome Anatomy 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 238000002864 sequence alignment Methods 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 230000001568 sexual effect Effects 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 235000000346 sugar Nutrition 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 238000004114 suspension culture Methods 0.000 description 1
- 230000005737 synergistic response Effects 0.000 description 1
- 230000002123 temporal effect Effects 0.000 description 1
- UZKQTCBAMSWPJD-UQCOIBPSSA-N trans-Zeatin Natural products OCC(/C)=C\CNC1=NC=NC2=C1N=CN2 UZKQTCBAMSWPJD-UQCOIBPSSA-N 0.000 description 1
- UZKQTCBAMSWPJD-FARCUNLSSA-N trans-zeatin Chemical compound OCC(/C)=C/CNC1=NC=NC2=C1N=CN2 UZKQTCBAMSWPJD-FARCUNLSSA-N 0.000 description 1
- 230000014621 translational initiation Effects 0.000 description 1
- 235000016788 valerian Nutrition 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
- 238000012800 visualization Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- 230000003442 weekly effect Effects 0.000 description 1
- 238000001262 western blot Methods 0.000 description 1
- 238000004383 yellowing Methods 0.000 description 1
- 229940023877 zeatin Drugs 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
- C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
- C12N15/8246—Non-starch polysaccharides, e.g. cellulose, fructans, levans
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
- C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
- C12N9/1051—Hexosyltransferases (2.4.1)
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Nutrition Science (AREA)
- Medicinal Chemistry (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US9700808P | 2008-09-15 | 2008-09-15 | |
| AU2009902230A AU2009902230A0 (en) | 2009-05-18 | Plant Technology (2) |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| TW201014909A TW201014909A (en) | 2010-04-16 |
| TWI534265B true TWI534265B (zh) | 2016-05-21 |
Family
ID=42004736
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| TW098130928A TWI534265B (zh) | 2008-09-15 | 2009-09-14 | 用於操縱植物之光合細胞中果聚糖生合成之方法、基因建構體及基因轉殖植物細胞 |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US9890387B2 (enExample) |
| EP (1) | EP2337848A4 (enExample) |
| JP (2) | JP5805534B2 (enExample) |
| AR (2) | AR073593A1 (enExample) |
| AU (1) | AU2009291522B2 (enExample) |
| CA (1) | CA2737059C (enExample) |
| MX (1) | MX2011002786A (enExample) |
| NZ (2) | NZ603035A (enExample) |
| TW (1) | TWI534265B (enExample) |
| UY (1) | UY32117A (enExample) |
| WO (1) | WO2010028456A1 (enExample) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9840695B2 (en) | 2009-04-28 | 2017-12-12 | Agriculture Victoria Services Pty Ltd | Plant technology |
| CN102811617A (zh) | 2010-01-22 | 2012-12-05 | 拜耳知识产权有限责任公司 | 杀螨和/或杀虫活性物质结合物 |
| US9265252B2 (en) | 2011-08-10 | 2016-02-23 | Bayer Intellectual Property Gmbh | Active compound combinations comprising specific tetramic acid derivatives |
| HUP1400505A2 (en) * | 2011-11-03 | 2015-03-02 | Syngenta Participations Ag | Polynucleotides, polypeptides and methods for enhancing photossimilation in plants |
| WO2021079297A1 (en) * | 2019-10-25 | 2021-04-29 | Agresearch Limited | Methods for improving photosynthetic organisms |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU5843794A (en) * | 1992-12-28 | 1994-07-19 | Stichting Scheikundig Onderzoek In Nederland | Method for obtaining transgenic plants showing a modified fructan pattern |
| NL1000064C1 (nl) * | 1994-07-08 | 1996-01-08 | Stichting Scheikundig Onderzoe | Produktie van oligosacchariden in transgene planten. |
| IN1997CH00924A (en) * | 1996-05-03 | 2005-03-04 | Syngenta Mogen Bv | Regulating metabolism by modifying the level of trehalose-6-phosphate |
| US6365800B1 (en) * | 1998-03-12 | 2002-04-02 | E. I. Du Pont Nemours & Company | Transgenic crops accumulating fructose polymers and methods for their production |
| EP0952222A1 (en) * | 1998-04-17 | 1999-10-27 | Centrum Voor Plantenveredelings- En Reproduktieonderzoek (Cpro-Dlo) | Transgenic plants presenting a modified inulin producing profile |
| MXPA01001049A (es) * | 1998-07-30 | 2002-08-20 | Metabolix Inc | Enzimas para produccion de biopolimeros. |
| AUPQ815500A0 (en) * | 2000-06-14 | 2000-07-06 | Molecular Plant Breeding Nominees Ltd | Modification of fructan biosynthesis |
| AUPQ994600A0 (en) * | 2000-09-06 | 2000-09-28 | Agriculture Victoria Services Pty Ltd | Manipulation of plant senescene |
| US6791015B2 (en) * | 2000-10-30 | 2004-09-14 | E. I. Du Pont De Nemours And Company | Fructan biosynthetic enzymes |
| EP1874934B1 (en) * | 2005-04-19 | 2013-11-27 | University of Kentucky Research Foundation | Transformed plants accumulating terpenes |
| JP4714894B2 (ja) * | 2005-06-22 | 2011-06-29 | 独立行政法人農業・食品産業技術総合研究機構 | 耐冷性植物及びその開発方法 |
| JP5257880B2 (ja) * | 2008-02-29 | 2013-08-07 | 独立行政法人農業・食品産業技術総合研究機構 | 植物由来高重合度フルクタン合成スクロース：フルクタン−６−フルクトシルトランスフェラーゼ遺伝子 |
-
2009
- 2009-09-14 JP JP2011526355A patent/JP5805534B2/ja not_active Expired - Fee Related
- 2009-09-14 CA CA2737059A patent/CA2737059C/en active Active
- 2009-09-14 AU AU2009291522A patent/AU2009291522B2/en active Active
- 2009-09-14 US US13/063,992 patent/US9890387B2/en active Active
- 2009-09-14 MX MX2011002786A patent/MX2011002786A/es active IP Right Grant
- 2009-09-14 NZ NZ603035A patent/NZ603035A/en not_active IP Right Cessation
- 2009-09-14 UY UY0001032117A patent/UY32117A/es active IP Right Grant
- 2009-09-14 TW TW098130928A patent/TWI534265B/zh not_active IP Right Cessation
- 2009-09-14 NZ NZ591777A patent/NZ591777A/xx not_active IP Right Cessation
- 2009-09-14 WO PCT/AU2009/001211 patent/WO2010028456A1/en not_active Ceased
- 2009-09-14 EP EP09812549A patent/EP2337848A4/en not_active Withdrawn
- 2009-09-15 AR ARP090103539A patent/AR073593A1/es active IP Right Grant
-
2015
- 2015-06-02 JP JP2015112338A patent/JP6224030B2/ja not_active Expired - Fee Related
-
2021
- 2021-04-27 AR ARP210101128A patent/AR122447A2/es unknown
Also Published As
| Publication number | Publication date |
|---|---|
| US9890387B2 (en) | 2018-02-13 |
| CA2737059A1 (en) | 2010-03-18 |
| US20110277187A1 (en) | 2011-11-10 |
| AR073593A1 (es) | 2010-11-17 |
| EP2337848A1 (en) | 2011-06-29 |
| WO2010028456A1 (en) | 2010-03-18 |
| AU2009291522A1 (en) | 2010-03-18 |
| JP5805534B2 (ja) | 2015-11-04 |
| NZ591777A (en) | 2012-12-21 |
| CA2737059C (en) | 2020-01-07 |
| AR122447A2 (es) | 2022-09-14 |
| MX2011002786A (es) | 2011-09-21 |
| NZ603035A (en) | 2014-01-31 |
| JP2012501662A (ja) | 2012-01-26 |
| JP6224030B2 (ja) | 2017-11-01 |
| TW201014909A (en) | 2010-04-16 |
| UY32117A (es) | 2010-03-26 |
| EP2337848A4 (en) | 2012-05-09 |
| AU2009291522B2 (en) | 2015-09-03 |
| JP2015154789A (ja) | 2015-08-27 |
| BRPI0913562A2 (pt) | 2015-08-04 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US10450580B2 (en) | Transcriptional regulation for improved plant productivity | |
| US9840695B2 (en) | Plant technology | |
| JP2002531096A (ja) | 色素体を形質転換するための方法 | |
| CN110408650A (zh) | NOR-like1基因及其编码的蛋白质在调控番茄果实产量中的应用 | |
| JP6224030B2 (ja) | フルクタン生合成の修飾、植物バイオマスの増大、および植物における生化学的経路の生産性を増強する方法 | |
| AU2010256356B2 (en) | Isolation and targeted suppression of lignin biosynthetic genes from sugarcane | |
| CN111434679B (zh) | 株型相关蛋白在调控植物株型中的应用 | |
| US10006039B2 (en) | Production of oil in vegetative tissues | |
| CA2299388A1 (en) | Processes for increasing the yield in plants | |
| CN107058317B (zh) | 一种花粉特异性启动子及其应用 | |
| WO2001027241A2 (en) | Method of introducing a plurality of genes into plants | |
| EP2348109A1 (en) | Genes having activity of promoting endoreduplication | |
| AU2015264827B2 (en) | Modification of fructan biosynthesis, increasing plant biomass, and enhancing productivity of biochemical pathways in a plant (2) | |
| CN110317826B (zh) | 调控PvGRF9含量或活性的物质在调控植物茎生长发育中的应用 | |
| US7112723B2 (en) | Globulin 2 regulatory region and method of using same | |
| US7109390B2 (en) | Alternative splicing factors polynucleotides polypeptides and uses therof | |
| US8642749B2 (en) | Regulatory region preferentially expressing to seed embryo and method of using same | |
| CN110373425A (zh) | NOR-like1基因及其编码的蛋白质在调控番茄果实采后失水中的应用 | |
| CN100424177C (zh) | 融合杀虫基因cryci及其应用 | |
| EP1228216A2 (en) | Plants with a modified flower and seed development | |
| CN107739403A (zh) | 一种与植物开花期相关的蛋白及其编码基因与应用 | |
| KR101370768B1 (ko) | 식물체 내 액포로부터 세포질로의 자당 수송 증가용 조성물 | |
| BRPI0913562B1 (pt) | Método para intensificar a produtividade de uma via biossintética de frutana e construto genético | |
| CZ2000451A3 (cs) | Způsob zvýšení výnosů rostlin |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MM4A | Annulment or lapse of patent due to non-payment of fees |