CA2737059C - Modification of fructan biosynthesis, increasing plant biomass, and enhancing productivity of biochemical pathways in a plant - Google Patents
Modification of fructan biosynthesis, increasing plant biomass, and enhancing productivity of biochemical pathways in a plant Download PDFInfo
- Publication number
- CA2737059C CA2737059C CA2737059A CA2737059A CA2737059C CA 2737059 C CA2737059 C CA 2737059C CA 2737059 A CA2737059 A CA 2737059A CA 2737059 A CA2737059 A CA 2737059A CA 2737059 C CA2737059 C CA 2737059C
- Authority
- CA
- Canada
- Prior art keywords
- plant
- fusion
- plants
- fft
- sst
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 229920002670 Fructan Polymers 0.000 title claims abstract description 197
- 239000002028 Biomass Substances 0.000 title claims abstract description 56
- 230000015572 biosynthetic process Effects 0.000 title claims abstract description 51
- 230000002708 enhancing effect Effects 0.000 title claims abstract description 18
- 230000001965 increasing effect Effects 0.000 title abstract description 44
- 230000008238 biochemical pathway Effects 0.000 title abstract description 18
- 230000004048 modification Effects 0.000 title abstract description 10
- 238000012986 modification Methods 0.000 title abstract description 10
- 150000007523 nucleic acids Chemical class 0.000 claims abstract description 86
- 238000000034 method Methods 0.000 claims abstract description 76
- 108020004707 nucleic acids Proteins 0.000 claims abstract description 74
- 102000039446 nucleic acids Human genes 0.000 claims abstract description 74
- 108020001507 fusion proteins Proteins 0.000 claims abstract description 37
- 102000037865 fusion proteins Human genes 0.000 claims abstract description 37
- 210000001916 photosynthetic cell Anatomy 0.000 claims abstract description 33
- 230000004927 fusion Effects 0.000 claims description 261
- 230000009261 transgenic effect Effects 0.000 claims description 202
- 108090000623 proteins and genes Proteins 0.000 claims description 175
- 230000001105 regulatory effect Effects 0.000 claims description 68
- 210000004027 cell Anatomy 0.000 claims description 66
- 102000004190 Enzymes Human genes 0.000 claims description 64
- 108090000790 Enzymes Proteins 0.000 claims description 64
- 230000002068 genetic effect Effects 0.000 claims description 63
- 101001074841 Allium cepa Bifunctional 6(G)-fructosyltransferase/2,1-fructan:2,1-fructan 1-fructosyltransferase Proteins 0.000 claims description 40
- 101001074840 Asparagus officinalis 6(G)-fructosyltransferase Proteins 0.000 claims description 31
- 230000001851 biosynthetic effect Effects 0.000 claims description 31
- 101100534435 Festuca arundinacea 1-SST gene Proteins 0.000 claims description 25
- 150000001720 carbohydrates Chemical class 0.000 claims description 21
- 230000009758 senescence Effects 0.000 claims description 17
- 101150078498 MYB gene Proteins 0.000 claims description 14
- UQHKFADEQIVWID-UHFFFAOYSA-N cytokinin Natural products C1=NC=2C(NCC=C(CO)C)=NC=NC=2N1C1CC(O)C(CO)O1 UQHKFADEQIVWID-UHFFFAOYSA-N 0.000 claims description 14
- 239000004062 cytokinin Substances 0.000 claims description 14
- 230000006696 biosynthetic metabolic pathway Effects 0.000 claims description 12
- 230000002786 root growth Effects 0.000 abstract description 7
- 241000196324 Embryophyta Species 0.000 description 375
- 108010042889 Inulosucrase Proteins 0.000 description 327
- 240000004296 Lolium perenne Species 0.000 description 114
- 230000014509 gene expression Effects 0.000 description 107
- 239000012634 fragment Substances 0.000 description 100
- 239000013598 vector Substances 0.000 description 92
- 230000009466 transformation Effects 0.000 description 61
- 244000098338 Triticum aestivum Species 0.000 description 57
- ZFTFOHBYVDOAMH-XNOIKFDKSA-N (2r,3s,4s,5r)-5-[[(2r,3s,4s,5r)-5-[[(2r,3s,4s,5r)-3,4-dihydroxy-2,5-bis(hydroxymethyl)oxolan-2-yl]oxymethyl]-3,4-dihydroxy-2-(hydroxymethyl)oxolan-2-yl]oxymethyl]-2-(hydroxymethyl)oxolane-2,3,4-triol Chemical class O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)OC[C@@H]1[C@@H](O)[C@H](O)[C@](CO)(OC[C@@H]2[C@H]([C@H](O)[C@@](O)(CO)O2)O)O1 ZFTFOHBYVDOAMH-XNOIKFDKSA-N 0.000 description 45
- 239000002773 nucleotide Substances 0.000 description 43
- 125000003729 nucleotide group Chemical group 0.000 description 43
- 230000012010 growth Effects 0.000 description 39
- 230000000243 photosynthetic effect Effects 0.000 description 37
- 229930006000 Sucrose Natural products 0.000 description 35
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 35
- 239000005720 sucrose Substances 0.000 description 35
- 102000004169 proteins and genes Human genes 0.000 description 34
- 241000234643 Festuca arundinacea Species 0.000 description 32
- 238000004519 manufacturing process Methods 0.000 description 32
- 210000001519 tissue Anatomy 0.000 description 32
- 108020004414 DNA Proteins 0.000 description 29
- 241000219194 Arabidopsis Species 0.000 description 28
- 235000021307 Triticum Nutrition 0.000 description 28
- 239000003550 marker Substances 0.000 description 28
- 239000013612 plasmid Substances 0.000 description 28
- 241000209082 Lolium Species 0.000 description 25
- 108700019146 Transgenes Proteins 0.000 description 22
- 125000003275 alpha amino acid group Chemical group 0.000 description 22
- 150000001413 amino acids Chemical class 0.000 description 21
- 101000702488 Rattus norvegicus High affinity cationic amino acid transporter 1 Proteins 0.000 description 20
- 229940024606 amino acid Drugs 0.000 description 20
- 238000012512 characterization method Methods 0.000 description 20
- 238000003752 polymerase chain reaction Methods 0.000 description 20
- 238000009825 accumulation Methods 0.000 description 19
- 238000004458 analytical method Methods 0.000 description 19
- 239000002299 complementary DNA Substances 0.000 description 19
- 238000005516 engineering process Methods 0.000 description 19
- 241000209504 Poaceae Species 0.000 description 18
- 108020004705 Codon Proteins 0.000 description 17
- 235000014633 carbohydrates Nutrition 0.000 description 17
- 238000010367 cloning Methods 0.000 description 17
- 241000589158 Agrobacterium Species 0.000 description 16
- 244000061456 Solanum tuberosum Species 0.000 description 16
- 240000008042 Zea mays Species 0.000 description 16
- 238000000338 in vitro Methods 0.000 description 16
- 108091028043 Nucleic acid sequence Proteins 0.000 description 15
- 235000002595 Solanum tuberosum Nutrition 0.000 description 15
- 230000037361 pathway Effects 0.000 description 15
- 108091008146 restriction endonucleases Proteins 0.000 description 15
- 238000006467 substitution reaction Methods 0.000 description 15
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 14
- 244000061176 Nicotiana tabacum Species 0.000 description 14
- 230000001404 mediated effect Effects 0.000 description 14
- 241000219195 Arabidopsis thaliana Species 0.000 description 13
- 101000981789 Arabidopsis thaliana Transcription factor MYB32 Proteins 0.000 description 13
- 241000701489 Cauliflower mosaic virus Species 0.000 description 13
- 241001268782 Paspalum dilatatum Species 0.000 description 13
- 244000042324 Trifolium repens Species 0.000 description 13
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 13
- 230000000694 effects Effects 0.000 description 13
- 108090000765 processed proteins & peptides Proteins 0.000 description 13
- 239000000047 product Substances 0.000 description 13
- 238000005215 recombination Methods 0.000 description 13
- 108700007698 Genetic Terminator Regions Proteins 0.000 description 12
- 240000007594 Oryza sativa Species 0.000 description 12
- 235000007164 Oryza sativa Nutrition 0.000 description 12
- 229920001184 polypeptide Polymers 0.000 description 12
- 102000004196 processed proteins & peptides Human genes 0.000 description 12
- 230000006798 recombination Effects 0.000 description 12
- 108700026244 Open Reading Frames Proteins 0.000 description 11
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 description 11
- 238000002474 experimental method Methods 0.000 description 11
- 230000014634 leaf senescence Effects 0.000 description 11
- 235000009973 maize Nutrition 0.000 description 11
- 230000008929 regeneration Effects 0.000 description 11
- 238000011069 regeneration method Methods 0.000 description 11
- 235000013540 Trifolium repens var repens Nutrition 0.000 description 10
- 238000012742 biochemical analysis Methods 0.000 description 10
- 230000000408 embryogenic effect Effects 0.000 description 10
- 241001233957 eudicotyledons Species 0.000 description 10
- 239000004459 forage Substances 0.000 description 10
- 230000002441 reversible effect Effects 0.000 description 10
- 241000894007 species Species 0.000 description 10
- 238000012546 transfer Methods 0.000 description 10
- VAWYEUIPHLMNNF-OESPXIITSA-N 1-kestose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)OC[C@]1(O[C@@H]2[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O2)O)[C@@H](O)[C@H](O)[C@@H](CO)O1 VAWYEUIPHLMNNF-OESPXIITSA-N 0.000 description 9
- GIUOHBJZYJAZNP-DVZCMHTBSA-N 1-kestose Natural products OC[C@@H]1O[C@](CO)(OC[C@]2(O[C@H]3O[C@H](CO)[C@@H](O)[C@H](O)[C@H]3O)O[C@@H](O)[C@@H](O)[C@@H]2O)[C@@H](O)[C@@H]1O GIUOHBJZYJAZNP-DVZCMHTBSA-N 0.000 description 9
- 240000000385 Brassica napus var. napus Species 0.000 description 9
- 230000003115 biocidal effect Effects 0.000 description 9
- 238000002955 isolation Methods 0.000 description 9
- VAWYEUIPHLMNNF-UHFFFAOYSA-N kestotriose Natural products OC1C(O)C(CO)OC1(CO)OCC1(OC2C(C(O)C(O)C(CO)O2)O)C(O)C(O)C(CO)O1 VAWYEUIPHLMNNF-UHFFFAOYSA-N 0.000 description 9
- 239000000203 mixture Substances 0.000 description 9
- 239000002245 particle Substances 0.000 description 9
- 235000009566 rice Nutrition 0.000 description 9
- 235000014698 Brassica juncea var multisecta Nutrition 0.000 description 8
- 235000006008 Brassica napus var napus Nutrition 0.000 description 8
- 235000006618 Brassica rapa subsp oleifera Nutrition 0.000 description 8
- 235000004977 Brassica sinapistrum Nutrition 0.000 description 8
- 241000234642 Festuca Species 0.000 description 8
- DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 description 8
- 108010051210 beta-Fructofuranosidase Proteins 0.000 description 8
- 230000003111 delayed effect Effects 0.000 description 8
- 235000011073 invertase Nutrition 0.000 description 8
- 239000002689 soil Substances 0.000 description 8
- 230000035882 stress Effects 0.000 description 8
- 241000508723 Festuca rubra Species 0.000 description 7
- 244000100545 Lolium multiflorum Species 0.000 description 7
- 108090000848 Ubiquitin Proteins 0.000 description 7
- 102000044159 Ubiquitin Human genes 0.000 description 7
- 238000003556 assay Methods 0.000 description 7
- 230000008901 benefit Effects 0.000 description 7
- 239000013604 expression vector Substances 0.000 description 7
- 101150012864 ipt gene Proteins 0.000 description 7
- 238000007479 molecular analysis Methods 0.000 description 7
- 210000001938 protoplast Anatomy 0.000 description 7
- 230000001052 transient effect Effects 0.000 description 7
- 235000010469 Glycine max Nutrition 0.000 description 6
- 244000068988 Glycine max Species 0.000 description 6
- 101710181960 Sucrose:sucrose 1-fructosyltransferase Proteins 0.000 description 6
- 238000007792 addition Methods 0.000 description 6
- 238000006243 chemical reaction Methods 0.000 description 6
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 6
- 210000002257 embryonic structure Anatomy 0.000 description 6
- 101150029559 hph gene Proteins 0.000 description 6
- 230000001939 inductive effect Effects 0.000 description 6
- 238000002347 injection Methods 0.000 description 6
- 239000007924 injection Substances 0.000 description 6
- 239000001573 invertase Substances 0.000 description 6
- 210000000056 organ Anatomy 0.000 description 6
- 238000009304 pastoral farming Methods 0.000 description 6
- 210000004767 rumen Anatomy 0.000 description 6
- 239000000758 substrate Substances 0.000 description 6
- YAHZABJORDUQGO-NQXXGFSBSA-N D-ribulose 1,5-bisphosphate Chemical compound OP(=O)(O)OC[C@@H](O)[C@@H](O)C(=O)COP(O)(O)=O YAHZABJORDUQGO-NQXXGFSBSA-N 0.000 description 5
- YQYJSBFKSSDGFO-UHFFFAOYSA-N Epihygromycin Natural products OC1C(O)C(C(=O)C)OC1OC(C(=C1)O)=CC=C1C=C(C)C(=O)NC1C(O)C(O)C2OCOC2C1O YQYJSBFKSSDGFO-UHFFFAOYSA-N 0.000 description 5
- 244000299507 Gossypium hirsutum Species 0.000 description 5
- 241000209510 Liliopsida Species 0.000 description 5
- 241000227653 Lycopersicon Species 0.000 description 5
- 241001465754 Metazoa Species 0.000 description 5
- 240000000111 Saccharum officinarum Species 0.000 description 5
- 235000007201 Saccharum officinarum Nutrition 0.000 description 5
- 238000005571 anion exchange chromatography Methods 0.000 description 5
- 239000003242 anti bacterial agent Substances 0.000 description 5
- 230000033228 biological regulation Effects 0.000 description 5
- 235000013339 cereals Nutrition 0.000 description 5
- 210000003763 chloroplast Anatomy 0.000 description 5
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 5
- 239000010931 gold Substances 0.000 description 5
- 229910052737 gold Inorganic materials 0.000 description 5
- 230000007062 hydrolysis Effects 0.000 description 5
- 238000006460 hydrolysis reaction Methods 0.000 description 5
- 239000000463 material Substances 0.000 description 5
- 230000002103 transcriptional effect Effects 0.000 description 5
- 230000001131 transforming effect Effects 0.000 description 5
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 4
- WFPZSXYXPSUOPY-ROYWQJLOSA-N ADP alpha-D-glucoside Chemical compound C([C@H]1O[C@H]([C@@H]([C@@H]1O)O)N1C=2N=CN=C(C=2N=C1)N)OP(O)(=O)OP(O)(=O)O[C@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O WFPZSXYXPSUOPY-ROYWQJLOSA-N 0.000 description 4
- 241000589155 Agrobacterium tumefaciens Species 0.000 description 4
- 244000025254 Cannabis sativa Species 0.000 description 4
- 108091035707 Consensus sequence Proteins 0.000 description 4
- 229920000742 Cotton Polymers 0.000 description 4
- 241000234645 Festuca pratensis Species 0.000 description 4
- 235000007340 Hordeum vulgare Nutrition 0.000 description 4
- 240000005979 Hordeum vulgare Species 0.000 description 4
- 102000004020 Oxygenases Human genes 0.000 description 4
- 108090000417 Oxygenases Proteins 0.000 description 4
- 108010003581 Ribulose-bisphosphate carboxylase Proteins 0.000 description 4
- 230000036579 abiotic stress Effects 0.000 description 4
- 238000000246 agarose gel electrophoresis Methods 0.000 description 4
- 239000003795 chemical substances by application Substances 0.000 description 4
- 229930002868 chlorophyll a Natural products 0.000 description 4
- 229930002869 chlorophyll b Natural products 0.000 description 4
- NSMUHPMZFPKNMZ-VBYMZDBQSA-M chlorophyll b Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C=O)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 NSMUHPMZFPKNMZ-VBYMZDBQSA-M 0.000 description 4
- 230000018044 dehydration Effects 0.000 description 4
- 238000006297 dehydration reaction Methods 0.000 description 4
- 238000012217 deletion Methods 0.000 description 4
- 230000037430 deletion Effects 0.000 description 4
- 238000001212 derivatisation Methods 0.000 description 4
- 230000029087 digestion Effects 0.000 description 4
- 235000013399 edible fruits Nutrition 0.000 description 4
- 230000005714 functional activity Effects 0.000 description 4
- 239000011521 glass Substances 0.000 description 4
- 238000011534 incubation Methods 0.000 description 4
- 238000001764 infiltration Methods 0.000 description 4
- 239000011159 matrix material Substances 0.000 description 4
- 230000004060 metabolic process Effects 0.000 description 4
- 230000037039 plant physiology Effects 0.000 description 4
- 239000013600 plasmid vector Substances 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 230000008672 reprogramming Effects 0.000 description 4
- 238000012216 screening Methods 0.000 description 4
- 230000004083 survival effect Effects 0.000 description 4
- 238000003786 synthesis reaction Methods 0.000 description 4
- 239000003104 tissue culture media Substances 0.000 description 4
- UZKQTCBAMSWPJD-UQCOIBPSSA-N trans-Zeatin Natural products OCC(/C)=C\CNC1=NC=NC2=C1N=CN2 UZKQTCBAMSWPJD-UQCOIBPSSA-N 0.000 description 4
- 230000010474 transient expression Effects 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 239000001707 (E,7R,11R)-3,7,11,15-tetramethylhexadec-2-en-1-ol Substances 0.000 description 3
- 101710112812 14-3-3 protein Proteins 0.000 description 3
- WFPZSXYXPSUOPY-UHFFFAOYSA-N ADP-mannose Natural products C1=NC=2C(N)=NC=NC=2N1C(C(C1O)O)OC1COP(O)(=O)OP(O)(=O)OC1OC(CO)C(O)C(O)C1O WFPZSXYXPSUOPY-UHFFFAOYSA-N 0.000 description 3
- 229920000936 Agarose Polymers 0.000 description 3
- 244000291564 Allium cepa Species 0.000 description 3
- 235000002732 Allium cepa var. cepa Nutrition 0.000 description 3
- 241000218631 Coniferophyta Species 0.000 description 3
- 108010031186 Glycoside Hydrolases Proteins 0.000 description 3
- 102000005744 Glycoside Hydrolases Human genes 0.000 description 3
- 108010033040 Histones Proteins 0.000 description 3
- 235000003228 Lactuca sativa Nutrition 0.000 description 3
- 240000008415 Lactuca sativa Species 0.000 description 3
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 3
- 240000004658 Medicago sativa Species 0.000 description 3
- 235000017587 Medicago sativa ssp. sativa Nutrition 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 241000207746 Nicotiana benthamiana Species 0.000 description 3
- 240000007377 Petunia x hybrida Species 0.000 description 3
- BLUHKGOSFDHHGX-UHFFFAOYSA-N Phytol Natural products CC(C)CCCC(C)CCCC(C)CCCC(C)C=CO BLUHKGOSFDHHGX-UHFFFAOYSA-N 0.000 description 3
- 241000282849 Ruminantia Species 0.000 description 3
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 3
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 3
- 240000003829 Sorghum propinquum Species 0.000 description 3
- 235000011684 Sorghum saccharatum Nutrition 0.000 description 3
- HNZBNQYXWOLKBA-UHFFFAOYSA-N Tetrahydrofarnesol Natural products CC(C)CCCC(C)CCCC(C)=CCO HNZBNQYXWOLKBA-UHFFFAOYSA-N 0.000 description 3
- 241000700605 Viruses Species 0.000 description 3
- 235000007244 Zea mays Nutrition 0.000 description 3
- 108010050516 adenylate isopentenyltransferase Proteins 0.000 description 3
- BOTWFXYSPFMFNR-OALUTQOASA-N all-rac-phytol Natural products CC(C)CCC[C@H](C)CCC[C@H](C)CCCC(C)=CCO BOTWFXYSPFMFNR-OALUTQOASA-N 0.000 description 3
- 230000002759 chromosomal effect Effects 0.000 description 3
- 238000004520 electroporation Methods 0.000 description 3
- 239000000284 extract Substances 0.000 description 3
- BJHIKXHVCXFQLS-UYFOZJQFSA-N fructose group Chemical group OCC(=O)[C@@H](O)[C@H](O)[C@H](O)CO BJHIKXHVCXFQLS-UYFOZJQFSA-N 0.000 description 3
- 230000006698 induction Effects 0.000 description 3
- 244000144972 livestock Species 0.000 description 3
- 239000002609 medium Substances 0.000 description 3
- 108020004999 messenger RNA Proteins 0.000 description 3
- 230000029553 photosynthesis Effects 0.000 description 3
- 238000010672 photosynthesis Methods 0.000 description 3
- BOTWFXYSPFMFNR-PYDDKJGSSA-N phytol Chemical compound CC(C)CCC[C@@H](C)CCC[C@@H](C)CCC\C(C)=C\CO BOTWFXYSPFMFNR-PYDDKJGSSA-N 0.000 description 3
- 230000008635 plant growth Effects 0.000 description 3
- 238000003762 quantitative reverse transcription PCR Methods 0.000 description 3
- 210000002107 sheath cell Anatomy 0.000 description 3
- 239000000725 suspension Substances 0.000 description 3
- 238000013518 transcription Methods 0.000 description 3
- 230000035897 transcription Effects 0.000 description 3
- 102000004899 14-3-3 Proteins Human genes 0.000 description 2
- IAJOBQBIJHVGMQ-UHFFFAOYSA-N 2-amino-4-[hydroxy(methyl)phosphoryl]butanoic acid Chemical compound CP(O)(=O)CCC(N)C(O)=O IAJOBQBIJHVGMQ-UHFFFAOYSA-N 0.000 description 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- 241001327399 Andropogon gerardii Species 0.000 description 2
- 101000981772 Arabidopsis thaliana Transcription factor MYB3 Proteins 0.000 description 2
- 235000007319 Avena orientalis Nutrition 0.000 description 2
- 244000075850 Avena orientalis Species 0.000 description 2
- 241000283690 Bos taurus Species 0.000 description 2
- 235000011331 Brassica Nutrition 0.000 description 2
- 241000219198 Brassica Species 0.000 description 2
- 240000002791 Brassica napus Species 0.000 description 2
- 235000011293 Brassica napus Nutrition 0.000 description 2
- 108091026890 Coding region Proteins 0.000 description 2
- 241000380130 Ehrharta erecta Species 0.000 description 2
- 235000016164 Elymus triticoides Nutrition 0.000 description 2
- 108010042407 Endonucleases Proteins 0.000 description 2
- 102000004533 Endonucleases Human genes 0.000 description 2
- VGGSQFUCUMXWEO-UHFFFAOYSA-N Ethene Chemical compound C=C VGGSQFUCUMXWEO-UHFFFAOYSA-N 0.000 description 2
- 239000005977 Ethylene Substances 0.000 description 2
- 229930091371 Fructose Natural products 0.000 description 2
- 239000005715 Fructose Substances 0.000 description 2
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 2
- 108700023224 Glucose-1-phosphate adenylyltransferases Proteins 0.000 description 2
- 239000005561 Glufosinate Substances 0.000 description 2
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 2
- 206010020649 Hyperkeratosis Diseases 0.000 description 2
- 229920001202 Inulin Polymers 0.000 description 2
- 244000017020 Ipomoea batatas Species 0.000 description 2
- 235000002678 Ipomoea batatas Nutrition 0.000 description 2
- 241000235058 Komagataella pastoris Species 0.000 description 2
- HGCNKOLVKRAVHD-UHFFFAOYSA-N L-Met-L-Phe Natural products CSCCC(N)C(=O)NC(C(O)=O)CC1=CC=CC=C1 HGCNKOLVKRAVHD-UHFFFAOYSA-N 0.000 description 2
- COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 description 2
- 241000511731 Leymus Species 0.000 description 2
- 235000002262 Lycopersicon Nutrition 0.000 description 2
- 101150017476 MYB32 gene Proteins 0.000 description 2
- 108010052285 Membrane Proteins Proteins 0.000 description 2
- HGCNKOLVKRAVHD-RYUDHWBXSA-N Met-Phe Chemical compound CSCC[C@H](N)C(=O)N[C@H](C(O)=O)CC1=CC=CC=C1 HGCNKOLVKRAVHD-RYUDHWBXSA-N 0.000 description 2
- 240000003433 Miscanthus floridulus Species 0.000 description 2
- 108700005084 Multigene Family Proteins 0.000 description 2
- 241000772415 Neovison vison Species 0.000 description 2
- 241000208125 Nicotiana Species 0.000 description 2
- 241001520808 Panicum virgatum Species 0.000 description 2
- 244000130556 Pennisetum purpureum Species 0.000 description 2
- 244000081757 Phalaris arundinacea Species 0.000 description 2
- 108010068086 Polyubiquitin Proteins 0.000 description 2
- 108010029485 Protein Isoforms Proteins 0.000 description 2
- 102000001708 Protein Isoforms Human genes 0.000 description 2
- 238000011529 RT qPCR Methods 0.000 description 2
- 235000014704 Saccharum spontaneum Nutrition 0.000 description 2
- 240000005382 Saccharum spontaneum Species 0.000 description 2
- 241000209056 Secale Species 0.000 description 2
- 235000007238 Secale cereale Nutrition 0.000 description 2
- 101000959886 Solanum tuberosum Aspartic protease inhibitor 2 Proteins 0.000 description 2
- 101000959868 Solanum tuberosum Aspartic protease inhibitor 8 Proteins 0.000 description 2
- 241000746413 Spartina Species 0.000 description 2
- 235000009337 Spinacia oleracea Nutrition 0.000 description 2
- 244000300264 Spinacia oleracea Species 0.000 description 2
- 108010043934 Sucrose synthase Proteins 0.000 description 2
- 108091023040 Transcription factor Proteins 0.000 description 2
- 241000219793 Trifolium Species 0.000 description 2
- 235000015724 Trifolium pratense Nutrition 0.000 description 2
- 235000010729 Trifolium repens Nutrition 0.000 description 2
- 241000219870 Trifolium subterraneum Species 0.000 description 2
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 2
- 230000002378 acidificating effect Effects 0.000 description 2
- 101150010487 are gene Proteins 0.000 description 2
- 210000004436 artificial bacterial chromosome Anatomy 0.000 description 2
- 125000003118 aryl group Chemical group 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 239000002551 biofuel Substances 0.000 description 2
- 230000003197 catalytic effect Effects 0.000 description 2
- 229930002875 chlorophyll Natural products 0.000 description 2
- 235000019804 chlorophyll Nutrition 0.000 description 2
- 230000004186 co-expression Effects 0.000 description 2
- 238000010276 construction Methods 0.000 description 2
- 235000005822 corn Nutrition 0.000 description 2
- 230000001186 cumulative effect Effects 0.000 description 2
- 235000013365 dairy product Nutrition 0.000 description 2
- 230000001419 dependent effect Effects 0.000 description 2
- 230000024346 drought recovery Effects 0.000 description 2
- 239000003623 enhancer Substances 0.000 description 2
- 230000001747 exhibiting effect Effects 0.000 description 2
- 238000010195 expression analysis Methods 0.000 description 2
- 238000000855 fermentation Methods 0.000 description 2
- 230000004151 fermentation Effects 0.000 description 2
- 238000010230 functional analysis Methods 0.000 description 2
- 238000010363 gene targeting Methods 0.000 description 2
- 125000003630 glycyl group Chemical group [H]N([H])C([H])([H])C(*)=O 0.000 description 2
- 230000001744 histochemical effect Effects 0.000 description 2
- 238000009396 hybridization Methods 0.000 description 2
- 238000000126 in silico method Methods 0.000 description 2
- 238000003780 insertion Methods 0.000 description 2
- 230000037431 insertion Effects 0.000 description 2
- 230000003993 interaction Effects 0.000 description 2
- 230000002015 leaf growth Effects 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 230000002503 metabolic effect Effects 0.000 description 2
- 108010068488 methionylphenylalanine Proteins 0.000 description 2
- 235000013336 milk Nutrition 0.000 description 2
- 239000008267 milk Substances 0.000 description 2
- 210000004080 milk Anatomy 0.000 description 2
- 238000010369 molecular cloning Methods 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 210000002706 plastid Anatomy 0.000 description 2
- 238000006116 polymerization reaction Methods 0.000 description 2
- 238000004382 potting Methods 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 108020001580 protein domains Proteins 0.000 description 2
- 235000013526 red clover Nutrition 0.000 description 2
- 230000001850 reproductive effect Effects 0.000 description 2
- 230000004044 response Effects 0.000 description 2
- 238000003757 reverse transcription PCR Methods 0.000 description 2
- 238000004809 thin layer chromatography Methods 0.000 description 2
- UZKQTCBAMSWPJD-FARCUNLSSA-N trans-zeatin Chemical compound OCC(/C)=C/CNC1=NC=NC2=C1N=CN2 UZKQTCBAMSWPJD-FARCUNLSSA-N 0.000 description 2
- 238000010361 transduction Methods 0.000 description 2
- 230000026683 transduction Effects 0.000 description 2
- 238000001890 transfection Methods 0.000 description 2
- 238000011144 upstream manufacturing Methods 0.000 description 2
- 235000013311 vegetables Nutrition 0.000 description 2
- 230000003612 virological effect Effects 0.000 description 2
- 238000003809 water extraction Methods 0.000 description 2
- -1 (N/S)DPNG Proteins 0.000 description 1
- 239000005631 2,4-Dichlorophenoxyacetic acid Substances 0.000 description 1
- KCYOZNARADAZIZ-CWBQGUJCSA-N 2-[(2e,4e,6e,8e,10e,12e,14e)-15-(4,4,7a-trimethyl-2,5,6,7-tetrahydro-1-benzofuran-2-yl)-6,11-dimethylhexadeca-2,4,6,8,10,12,14-heptaen-2-yl]-4,4,7a-trimethyl-2,5,6,7-tetrahydro-1-benzofuran-6-ol Chemical compound O1C2(C)CC(O)CC(C)(C)C2=CC1C(\C)=C\C=C\C(\C)=C\C=C\C=C(/C)\C=C\C=C(/C)C1C=C2C(C)(C)CCCC2(C)O1 KCYOZNARADAZIZ-CWBQGUJCSA-N 0.000 description 1
- 101150033839 4 gene Proteins 0.000 description 1
- 101710145411 Acid beta-fructofuranosidase Proteins 0.000 description 1
- 101710197633 Actin-1 Proteins 0.000 description 1
- 102000007469 Actins Human genes 0.000 description 1
- 108010085238 Actins Proteins 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 241000589156 Agrobacterium rhizogenes Species 0.000 description 1
- 239000004382 Amylase Substances 0.000 description 1
- 101100439969 Arabidopsis thaliana CLPD gene Proteins 0.000 description 1
- 101100148680 Arabidopsis thaliana SAG12 gene Proteins 0.000 description 1
- 235000007558 Avena sp Nutrition 0.000 description 1
- 244000063299 Bacillus subtilis Species 0.000 description 1
- 235000014469 Bacillus subtilis Nutrition 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 241000219310 Beta vulgaris subsp. vulgaris Species 0.000 description 1
- 235000011299 Brassica oleracea var botrytis Nutrition 0.000 description 1
- 240000003259 Brassica oleracea var. botrytis Species 0.000 description 1
- 101100228196 Caenorhabditis elegans gly-4 gene Proteins 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 102000014914 Carrier Proteins Human genes 0.000 description 1
- 108091062157 Cis-regulatory element Proteins 0.000 description 1
- RGJOEKWQDUBAIZ-IBOSZNHHSA-N CoASH Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCS)O[C@H]1N1C2=NC=NC(N)=C2N=C1 RGJOEKWQDUBAIZ-IBOSZNHHSA-N 0.000 description 1
- 241000287937 Colinus Species 0.000 description 1
- KCYOZNARADAZIZ-PPBBKLJYSA-N Cryptochrome Natural products O[C@@H]1CC(C)(C)C=2[C@@](C)(O[C@H](/C(=C\C=C\C(=C/C=C/C=C(\C=C\C=C(\C)/[C@H]3O[C@@]4(C)C(C(C)(C)CCC4)=C3)/C)\C)/C)C=2)C1 KCYOZNARADAZIZ-PPBBKLJYSA-N 0.000 description 1
- 108010037139 Cryptochromes Proteins 0.000 description 1
- 241000195493 Cryptophyta Species 0.000 description 1
- 241000192700 Cyanobacteria Species 0.000 description 1
- 108010066133 D-octopine dehydrogenase Proteins 0.000 description 1
- 238000001712 DNA sequencing Methods 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 101100437498 Escherichia coli (strain K12) uidA gene Proteins 0.000 description 1
- 241000701959 Escherichia virus Lambda Species 0.000 description 1
- 241000206602 Eukaryota Species 0.000 description 1
- 108010060309 Glucuronidase Proteins 0.000 description 1
- 102000053187 Glucuronidase Human genes 0.000 description 1
- 235000009432 Gossypium hirsutum Nutrition 0.000 description 1
- 108091092195 Intron Proteins 0.000 description 1
- 108010025815 Kanamycin Kinase Proteins 0.000 description 1
- FAIXYKHYOGVFKA-UHFFFAOYSA-N Kinetin Natural products N=1C=NC=2N=CNC=2C=1N(C)C1=CC=CO1 FAIXYKHYOGVFKA-UHFFFAOYSA-N 0.000 description 1
- QEFRNWWLZKMPFJ-ZXPFJRLXSA-N L-methionine (R)-S-oxide Chemical compound C[S@@](=O)CC[C@H]([NH3+])C([O-])=O QEFRNWWLZKMPFJ-ZXPFJRLXSA-N 0.000 description 1
- QEFRNWWLZKMPFJ-UHFFFAOYSA-N L-methionine sulphoxide Natural products CS(=O)CCC(N)C(O)=O QEFRNWWLZKMPFJ-UHFFFAOYSA-N 0.000 description 1
- 102000003960 Ligases Human genes 0.000 description 1
- 108090000364 Ligases Proteins 0.000 description 1
- 241001480167 Lotus japonicus Species 0.000 description 1
- 241000218922 Magnoliophyta Species 0.000 description 1
- 240000003183 Manihot esculenta Species 0.000 description 1
- 235000016735 Manihot esculenta subsp esculenta Nutrition 0.000 description 1
- 102000000532 Methionine Sulfoxide Reductases Human genes 0.000 description 1
- 108060004795 Methyltransferase Proteins 0.000 description 1
- 108700011259 MicroRNAs Proteins 0.000 description 1
- NWBJYWHLCVSVIJ-UHFFFAOYSA-N N-benzyladenine Chemical compound N=1C=NC=2NC=NC=2C=1NCC1=CC=CC=C1 NWBJYWHLCVSVIJ-UHFFFAOYSA-N 0.000 description 1
- 235000006508 Nelumbo nucifera Nutrition 0.000 description 1
- 240000002853 Nelumbo nucifera Species 0.000 description 1
- 235000006510 Nelumbo pentapetala Nutrition 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 238000000636 Northern blotting Methods 0.000 description 1
- 101000880550 Oryza sativa subsp. japonica Calcium-dependent protein kinase 13 Proteins 0.000 description 1
- 101000908192 Oryza sativa subsp. japonica Calcium-dependent protein kinase 25 Proteins 0.000 description 1
- 101000884367 Oryza sativa subsp. japonica Calcium-dependent protein kinase 6 Proteins 0.000 description 1
- 108090000854 Oxidoreductases Proteins 0.000 description 1
- 102000004316 Oxidoreductases Human genes 0.000 description 1
- CBENFWSGALASAD-UHFFFAOYSA-N Ozone Chemical compound [O-][O+]=O CBENFWSGALASAD-UHFFFAOYSA-N 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 101150009729 Pal2 gene Proteins 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 108700023158 Phenylalanine ammonia-lyases Proteins 0.000 description 1
- 108090000679 Phytochrome Proteins 0.000 description 1
- 108010067969 Phytochrome A Proteins 0.000 description 1
- 108700001094 Plant Genes Proteins 0.000 description 1
- 239000002202 Polyethylene glycol Substances 0.000 description 1
- 102100037935 Polyubiquitin-C Human genes 0.000 description 1
- 102000001253 Protein Kinase Human genes 0.000 description 1
- 108010076504 Protein Sorting Signals Proteins 0.000 description 1
- 241000589516 Pseudomonas Species 0.000 description 1
- 101710165374 Putative helicase Proteins 0.000 description 1
- 101150041925 RBCS gene Proteins 0.000 description 1
- 101150051586 RIM21 gene Proteins 0.000 description 1
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 description 1
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 description 1
- 108700008625 Reporter Genes Proteins 0.000 description 1
- 241000589180 Rhizobium Species 0.000 description 1
- 101100438804 Rhodobacter capsulatus (strain ATCC BAA-309 / NBRC 16581 / SB1003) helB gene Proteins 0.000 description 1
- 238000002105 Southern blotting Methods 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 235000021536 Sugar beet Nutrition 0.000 description 1
- 102000040945 Transcription factor Human genes 0.000 description 1
- 101710195626 Transcriptional activator protein Proteins 0.000 description 1
- 102000004357 Transferases Human genes 0.000 description 1
- 108090000992 Transferases Proteins 0.000 description 1
- 235000007264 Triticum durum Nutrition 0.000 description 1
- 240000002805 Triticum turgidum Species 0.000 description 1
- 241000209143 Triticum turgidum subsp. durum Species 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 239000011543 agarose gel Substances 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 125000000539 amino acid group Chemical group 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 230000000692 anti-sense effect Effects 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 210000001106 artificial yeast chromosome Anatomy 0.000 description 1
- 229960001230 asparagine Drugs 0.000 description 1
- KCYOZNARADAZIZ-XZOHMNSDSA-N beta-cryptochrome Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C1OC2(C)CC(O)CC(C)(C)C2=C1)C=CC=C(/C)C3OC4(C)CCCC(C)(C)C4=C3 KCYOZNARADAZIZ-XZOHMNSDSA-N 0.000 description 1
- 108091008324 binding proteins Proteins 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 239000004464 cereal grain Substances 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- RGJOEKWQDUBAIZ-UHFFFAOYSA-N coenzime A Natural products OC1C(OP(O)(O)=O)C(COP(O)(=O)OP(O)(=O)OCC(C)(C)C(O)C(=O)NCCC(=O)NCCS)OC1N1C2=NC=NC(N)=C2N=C1 RGJOEKWQDUBAIZ-UHFFFAOYSA-N 0.000 description 1
- 239000005516 coenzyme A Substances 0.000 description 1
- 229940093530 coenzyme a Drugs 0.000 description 1
- 238000012790 confirmation Methods 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 230000034994 death Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- KDTSHFARGAKYJN-UHFFFAOYSA-N dephosphocoenzyme A Natural products OC1C(O)C(COP(O)(=O)OP(O)(=O)OCC(C)(C)C(O)C(=O)NCCC(=O)NCCS)OC1N1C2=NC=NC(N)=C2N=C1 KDTSHFARGAKYJN-UHFFFAOYSA-N 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 235000019621 digestibility Nutrition 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 230000008641 drought stress Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 230000006353 environmental stress Effects 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 101150021535 erd1 gene Proteins 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 101150054603 ft gene Proteins 0.000 description 1
- 238000003633 gene expression assay Methods 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 108010002685 hygromycin-B kinase Proteins 0.000 description 1
- 235000013666 improved nutrition Nutrition 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 230000000977 initiatory effect Effects 0.000 description 1
- JYJIGFIDKWBXDU-MNNPPOADSA-N inulin Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)OC[C@]1(OC[C@]2(OC[C@]3(OC[C@]4(OC[C@]5(OC[C@]6(OC[C@]7(OC[C@]8(OC[C@]9(OC[C@]%10(OC[C@]%11(OC[C@]%12(OC[C@]%13(OC[C@]%14(OC[C@]%15(OC[C@]%16(OC[C@]%17(OC[C@]%18(OC[C@]%19(OC[C@]%20(OC[C@]%21(OC[C@]%22(OC[C@]%23(OC[C@]%24(OC[C@]%25(OC[C@]%26(OC[C@]%27(OC[C@]%28(OC[C@]%29(OC[C@]%30(OC[C@]%31(OC[C@]%32(OC[C@]%33(OC[C@]%34(OC[C@]%35(OC[C@]%36(O[C@@H]%37[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O%37)O)[C@H]([C@H](O)[C@@H](CO)O%36)O)[C@H]([C@H](O)[C@@H](CO)O%35)O)[C@H]([C@H](O)[C@@H](CO)O%34)O)[C@H]([C@H](O)[C@@H](CO)O%33)O)[C@H]([C@H](O)[C@@H](CO)O%32)O)[C@H]([C@H](O)[C@@H](CO)O%31)O)[C@H]([C@H](O)[C@@H](CO)O%30)O)[C@H]([C@H](O)[C@@H](CO)O%29)O)[C@H]([C@H](O)[C@@H](CO)O%28)O)[C@H]([C@H](O)[C@@H](CO)O%27)O)[C@H]([C@H](O)[C@@H](CO)O%26)O)[C@H]([C@H](O)[C@@H](CO)O%25)O)[C@H]([C@H](O)[C@@H](CO)O%24)O)[C@H]([C@H](O)[C@@H](CO)O%23)O)[C@H]([C@H](O)[C@@H](CO)O%22)O)[C@H]([C@H](O)[C@@H](CO)O%21)O)[C@H]([C@H](O)[C@@H](CO)O%20)O)[C@H]([C@H](O)[C@@H](CO)O%19)O)[C@H]([C@H](O)[C@@H](CO)O%18)O)[C@H]([C@H](O)[C@@H](CO)O%17)O)[C@H]([C@H](O)[C@@H](CO)O%16)O)[C@H]([C@H](O)[C@@H](CO)O%15)O)[C@H]([C@H](O)[C@@H](CO)O%14)O)[C@H]([C@H](O)[C@@H](CO)O%13)O)[C@H]([C@H](O)[C@@H](CO)O%12)O)[C@H]([C@H](O)[C@@H](CO)O%11)O)[C@H]([C@H](O)[C@@H](CO)O%10)O)[C@H]([C@H](O)[C@@H](CO)O9)O)[C@H]([C@H](O)[C@@H](CO)O8)O)[C@H]([C@H](O)[C@@H](CO)O7)O)[C@H]([C@H](O)[C@@H](CO)O6)O)[C@H]([C@H](O)[C@@H](CO)O5)O)[C@H]([C@H](O)[C@@H](CO)O4)O)[C@H]([C@H](O)[C@@H](CO)O3)O)[C@H]([C@H](O)[C@@H](CO)O2)O)[C@@H](O)[C@H](O)[C@@H](CO)O1 JYJIGFIDKWBXDU-MNNPPOADSA-N 0.000 description 1
- 229940029339 inulin Drugs 0.000 description 1
- 125000001972 isopentyl group Chemical group [H]C([H])([H])C([H])(C([H])([H])[H])C([H])([H])C([H])([H])* 0.000 description 1
- 238000005304 joining Methods 0.000 description 1
- QANMHLXAZMSUEX-UHFFFAOYSA-N kinetin Chemical compound N=1C=NC=2N=CNC=2C=1NCC1=CC=CO1 QANMHLXAZMSUEX-UHFFFAOYSA-N 0.000 description 1
- 229960001669 kinetin Drugs 0.000 description 1
- AIHDCSAXVMAMJH-GFBKWZILSA-N levan Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)OC[C@@H]1[C@@H](O)[C@H](O)[C@](CO)(CO[C@@H]2[C@H]([C@H](O)[C@@](O)(CO)O2)O)O1 AIHDCSAXVMAMJH-GFBKWZILSA-N 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 230000004807 localization Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000000442 meristematic effect Effects 0.000 description 1
- 210000000473 mesophyll cell Anatomy 0.000 description 1
- 108010020410 methionine sulfoxide reductase Proteins 0.000 description 1
- 239000002679 microRNA Substances 0.000 description 1
- 238000002493 microarray Methods 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 238000001000 micrograph Methods 0.000 description 1
- 238000000520 microinjection Methods 0.000 description 1
- 230000000869 mutational effect Effects 0.000 description 1
- 239000013642 negative control Substances 0.000 description 1
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 1
- 108010058731 nopaline synthase Proteins 0.000 description 1
- 230000031787 nutrient reservoir activity Effects 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 230000036542 oxidative stress Effects 0.000 description 1
- UOJMTSCORVQOHS-UHFFFAOYSA-N pachypodol Natural products COc1cc(ccc1O)C2=C(C)C(=O)c3c(O)cc(C)cc3O2 UOJMTSCORVQOHS-UHFFFAOYSA-N 0.000 description 1
- 230000014306 paracrine signaling Effects 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 239000000137 peptide hydrolase inhibitor Substances 0.000 description 1
- 230000002688 persistence Effects 0.000 description 1
- 108010082527 phosphinothricin N-acetyltransferase Proteins 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 230000008119 pollen development Effects 0.000 description 1
- 238000002264 polyacrylamide gel electrophoresis Methods 0.000 description 1
- 230000008488 polyadenylation Effects 0.000 description 1
- 229920001223 polyethylene glycol Polymers 0.000 description 1
- 239000013641 positive control Substances 0.000 description 1
- 230000001323 posttranslational effect Effects 0.000 description 1
- 235000012015 potatoes Nutrition 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 230000004853 protein function Effects 0.000 description 1
- 108060006633 protein kinase Proteins 0.000 description 1
- 238000001273 protein sequence alignment Methods 0.000 description 1
- 238000000275 quality assurance Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000010076 replication Effects 0.000 description 1
- 230000003362 replicative effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000025474 response to light stimulus Effects 0.000 description 1
- 230000003938 response to stress Effects 0.000 description 1
- 101150025220 sacB gene Proteins 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 230000001932 seasonal effect Effects 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000002864 sequence alignment Methods 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 244000000000 soil microbiome Species 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000000638 solvent extraction Methods 0.000 description 1
- 238000009331 sowing Methods 0.000 description 1
- 125000006850 spacer group Chemical group 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000020936 starving conditions Nutrition 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 108010022290 sucrose - fructan 6-fructosyltransferase Proteins 0.000 description 1
- 235000000346 sugar Nutrition 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 238000004114 suspension culture Methods 0.000 description 1
- 230000005737 synergistic response Effects 0.000 description 1
- 230000008685 targeting Effects 0.000 description 1
- 230000002123 temporal effect Effects 0.000 description 1
- 150000003505 terpenes Chemical class 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- NGSWKAQJJWESNS-ZZXKWVIFSA-N trans-4-coumaric acid Chemical compound OC(=O)\C=C\C1=CC=C(O)C=C1 NGSWKAQJJWESNS-ZZXKWVIFSA-N 0.000 description 1
- 238000000844 transformation Methods 0.000 description 1
- 238000012250 transgenic expression Methods 0.000 description 1
- 238000013519 translation Methods 0.000 description 1
- 230000014621 translational initiation Effects 0.000 description 1
- 238000012800 visualization Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- 230000003442 weekly effect Effects 0.000 description 1
- 238000001262 western blot Methods 0.000 description 1
- 238000004383 yellowing Methods 0.000 description 1
- 229940023877 zeatin Drugs 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
- C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
- C12N15/8246—Non-starch polysaccharides, e.g. cellulose, fructans, levans
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
- C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
- C12N9/1051—Hexosyltransferases (2.4.1)
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Nutrition Science (AREA)
- Medicinal Chemistry (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US9700808P | 2008-09-15 | 2008-09-15 | |
| US61/097,008 | 2008-09-15 | ||
| AU2009902230A AU2009902230A0 (en) | 2009-05-18 | Plant Technology (2) | |
| AU2009902230 | 2009-05-18 | ||
| PCT/AU2009/001211 WO2010028456A1 (en) | 2008-09-15 | 2009-09-14 | Modification of fructan biosynthesis, increasing plant biomass, and enhancing productivity of biochemical pathways in a plant |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2737059A1 CA2737059A1 (en) | 2010-03-18 |
| CA2737059C true CA2737059C (en) | 2020-01-07 |
Family
ID=42004736
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2737059A Active CA2737059C (en) | 2008-09-15 | 2009-09-14 | Modification of fructan biosynthesis, increasing plant biomass, and enhancing productivity of biochemical pathways in a plant |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US9890387B2 (enExample) |
| EP (1) | EP2337848A4 (enExample) |
| JP (2) | JP5805534B2 (enExample) |
| AR (2) | AR073593A1 (enExample) |
| AU (1) | AU2009291522B2 (enExample) |
| CA (1) | CA2737059C (enExample) |
| MX (1) | MX2011002786A (enExample) |
| NZ (2) | NZ603035A (enExample) |
| TW (1) | TWI534265B (enExample) |
| UY (1) | UY32117A (enExample) |
| WO (1) | WO2010028456A1 (enExample) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9840695B2 (en) | 2009-04-28 | 2017-12-12 | Agriculture Victoria Services Pty Ltd | Plant technology |
| CN102811617A (zh) | 2010-01-22 | 2012-12-05 | 拜耳知识产权有限责任公司 | 杀螨和/或杀虫活性物质结合物 |
| US9265252B2 (en) | 2011-08-10 | 2016-02-23 | Bayer Intellectual Property Gmbh | Active compound combinations comprising specific tetramic acid derivatives |
| HUP1400505A2 (en) * | 2011-11-03 | 2015-03-02 | Syngenta Participations Ag | Polynucleotides, polypeptides and methods for enhancing photossimilation in plants |
| WO2021079297A1 (en) * | 2019-10-25 | 2021-04-29 | Agresearch Limited | Methods for improving photosynthetic organisms |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU5843794A (en) * | 1992-12-28 | 1994-07-19 | Stichting Scheikundig Onderzoek In Nederland | Method for obtaining transgenic plants showing a modified fructan pattern |
| NL1000064C1 (nl) * | 1994-07-08 | 1996-01-08 | Stichting Scheikundig Onderzoe | Produktie van oligosacchariden in transgene planten. |
| IN1997CH00924A (en) * | 1996-05-03 | 2005-03-04 | Syngenta Mogen Bv | Regulating metabolism by modifying the level of trehalose-6-phosphate |
| US6365800B1 (en) * | 1998-03-12 | 2002-04-02 | E. I. Du Pont Nemours & Company | Transgenic crops accumulating fructose polymers and methods for their production |
| EP0952222A1 (en) * | 1998-04-17 | 1999-10-27 | Centrum Voor Plantenveredelings- En Reproduktieonderzoek (Cpro-Dlo) | Transgenic plants presenting a modified inulin producing profile |
| MXPA01001049A (es) * | 1998-07-30 | 2002-08-20 | Metabolix Inc | Enzimas para produccion de biopolimeros. |
| AUPQ815500A0 (en) * | 2000-06-14 | 2000-07-06 | Molecular Plant Breeding Nominees Ltd | Modification of fructan biosynthesis |
| AUPQ994600A0 (en) * | 2000-09-06 | 2000-09-28 | Agriculture Victoria Services Pty Ltd | Manipulation of plant senescene |
| US6791015B2 (en) * | 2000-10-30 | 2004-09-14 | E. I. Du Pont De Nemours And Company | Fructan biosynthetic enzymes |
| EP1874934B1 (en) * | 2005-04-19 | 2013-11-27 | University of Kentucky Research Foundation | Transformed plants accumulating terpenes |
| JP4714894B2 (ja) * | 2005-06-22 | 2011-06-29 | 独立行政法人農業・食品産業技術総合研究機構 | 耐冷性植物及びその開発方法 |
| JP5257880B2 (ja) * | 2008-02-29 | 2013-08-07 | 独立行政法人農業・食品産業技術総合研究機構 | 植物由来高重合度フルクタン合成スクロース：フルクタン−６−フルクトシルトランスフェラーゼ遺伝子 |
-
2009
- 2009-09-14 JP JP2011526355A patent/JP5805534B2/ja not_active Expired - Fee Related
- 2009-09-14 CA CA2737059A patent/CA2737059C/en active Active
- 2009-09-14 AU AU2009291522A patent/AU2009291522B2/en active Active
- 2009-09-14 US US13/063,992 patent/US9890387B2/en active Active
- 2009-09-14 MX MX2011002786A patent/MX2011002786A/es active IP Right Grant
- 2009-09-14 NZ NZ603035A patent/NZ603035A/en not_active IP Right Cessation
- 2009-09-14 UY UY0001032117A patent/UY32117A/es active IP Right Grant
- 2009-09-14 TW TW098130928A patent/TWI534265B/zh not_active IP Right Cessation
- 2009-09-14 NZ NZ591777A patent/NZ591777A/xx not_active IP Right Cessation
- 2009-09-14 WO PCT/AU2009/001211 patent/WO2010028456A1/en not_active Ceased
- 2009-09-14 EP EP09812549A patent/EP2337848A4/en not_active Withdrawn
- 2009-09-15 AR ARP090103539A patent/AR073593A1/es active IP Right Grant
-
2015
- 2015-06-02 JP JP2015112338A patent/JP6224030B2/ja not_active Expired - Fee Related
-
2021
- 2021-04-27 AR ARP210101128A patent/AR122447A2/es unknown
Also Published As
| Publication number | Publication date |
|---|---|
| US9890387B2 (en) | 2018-02-13 |
| CA2737059A1 (en) | 2010-03-18 |
| US20110277187A1 (en) | 2011-11-10 |
| AR073593A1 (es) | 2010-11-17 |
| EP2337848A1 (en) | 2011-06-29 |
| WO2010028456A1 (en) | 2010-03-18 |
| AU2009291522A1 (en) | 2010-03-18 |
| JP5805534B2 (ja) | 2015-11-04 |
| NZ591777A (en) | 2012-12-21 |
| AR122447A2 (es) | 2022-09-14 |
| MX2011002786A (es) | 2011-09-21 |
| NZ603035A (en) | 2014-01-31 |
| JP2012501662A (ja) | 2012-01-26 |
| JP6224030B2 (ja) | 2017-11-01 |
| TW201014909A (en) | 2010-04-16 |
| UY32117A (es) | 2010-03-26 |
| TWI534265B (zh) | 2016-05-21 |
| EP2337848A4 (en) | 2012-05-09 |
| AU2009291522B2 (en) | 2015-09-03 |
| JP2015154789A (ja) | 2015-08-27 |
| BRPI0913562A2 (pt) | 2015-08-04 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US20220380790A1 (en) | Spatially modified gene expression in plants | |
| US9840695B2 (en) | Plant technology | |
| US10450580B2 (en) | Transcriptional regulation for improved plant productivity | |
| US9163233B2 (en) | Compositions and methods for modulating expression of gene products | |
| US11913008B2 (en) | Vector comprising sorghum terminator and method of use | |
| CA2737059C (en) | Modification of fructan biosynthesis, increasing plant biomass, and enhancing productivity of biochemical pathways in a plant | |
| MX2014007711A (es) | Metodos para mejorar rendimiento de cultivos. | |
| CA2931387A1 (en) | Drought tolerant plants | |
| AU2015264827B2 (en) | Modification of fructan biosynthesis, increasing plant biomass, and enhancing productivity of biochemical pathways in a plant (2) | |
| WO2009104181A1 (en) | Plants having genetically modified lignin content and methods of producing same | |
| BRPI0913562B1 (pt) | Método para intensificar a produtividade de uma via biossintética de frutana e construto genético | |
| CA2828931C (en) | Regulatory regions preferentially expressing in non-pollen plant tissue |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request |
Effective date: 20140724 |