TWI456061B - 豬水腫病疫苗 - Google Patents

豬水腫病疫苗 Download PDF

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Publication number
TWI456061B
TWI456061B TW097118119A TW97118119A TWI456061B TW I456061 B TWI456061 B TW I456061B TW 097118119 A TW097118119 A TW 097118119A TW 97118119 A TW97118119 A TW 97118119A TW I456061 B TWI456061 B TW I456061B
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Taiwan
Prior art keywords
dna
seq
dna construct
sequence represented
tobacco
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TW097118119A
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English (en)
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TW200914611A (en
Inventor
Kazutoshi Sawada
Kazuya Yoshida
Takeshi Matsui
Sou-Ichi Makino
Keiko Kawamoto
Original Assignee
Idemitsu Kosan Co
Nat Univ Corp Nara Inst
Nat University Corp Obihiro University Of Agriculture And Veterinary Medicine
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Application filed by Idemitsu Kosan Co, Nat Univ Corp Nara Inst, Nat University Corp Obihiro University Of Agriculture And Veterinary Medicine filed Critical Idemitsu Kosan Co
Publication of TW200914611A publication Critical patent/TW200914611A/zh
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Publication of TWI456061B publication Critical patent/TWI456061B/zh

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C12N15/8258—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon for the production of oral vaccines (antigens) or immunoglobulins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/02—Bacterial antigens
    • A61K39/025—Enterobacteriales, e.g. Enterobacter
    • A61K39/0258—Escherichia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P39/00—General protective or antinoxious agents
    • A61P39/02—Antidotes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/517—Plant cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/55—Medicinal preparations containing antigens or antibodies characterised by the host/recipient, e.g. newborn with maternal antibodies
    • A61K2039/552—Veterinary vaccine
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Mycology (AREA)
  • Epidemiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Oncology (AREA)
  • Communicable Diseases (AREA)
  • Toxicology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Claims (12)

  1. 一種DNA建構物，其特徵為包含編碼源自菸草之醇脫氫酶基因之5’-非轉譯區域；以及編碼連結於該區域且可表現之於胺基末端加成有源自菸草之分泌訊號胜肽，且於羧基末端加成有小胞體殘留訊號胜肽之Stx2e蛋白質的B次單元之DNA。
  2. 如申請專利範圍第1項之DNA建構物，其中上述小胞體殘留訊號胜肽於羧基末端包含以序列編號13表示之KDEL序列或以序列編號14表示之HDEL序列。
  3. 如申請專利範圍第1項之DNA建構物，其中源自菸草之醇脫氫酶基因之5’-非轉譯區域係具有序列編號1的鹼基序列的DNA。
  4. 如申請專利範圍第1項之DNA建構物，其中源自菸草之分泌訊號胜肽係被具有序列編號11的鹼基序列的DNA所編碼。
  5. 如申請專利範圍第1項之DNA建構物，其中Stx2e蛋白質的B次單元係具有：具有序列編號3的胺基酸序列。
  6. 如申請專利範圍第1項之DNA建構物，其係為包含以序列編號12所表示之鹼基序列的DNA或與具有以序列編號12所表示之鹼基序列的DNA在包含於2×SSC、42℃下雜化(hybridization)之嚴格條件下雜化之DNA。
  7. 如申請專利範圍第1項之DNA建構物，其係為具有以序列編號23、75、77、78、86任一者所表示之鹼基序列 的DNA或與具有以序列編號23、75、77、78、86任一者所表示之鹼基序列的DNA在包含於2×SSC、42℃下雜化之嚴格條件下雜化之DNA。
  8. 一種重組載體，其特徵為含有申請專利範圍第1至7項中任一項之DNA建構物。
  9. 一種轉形體，其特徵為係以申請專利範圍第8項之重組載體予以轉形者。
  10. 如申請專利範圍第9項之轉形體，其中該轉形體係轉形植物細胞或轉形植物。
  11. 如申請專利範圍第10項之轉形體，其中該植物為萵苣(Lactuca sativa)。
  12. 一種豬水腫病疫苗，其特徵為包含申請專利範圍第9至11項中任一項之轉形體，且不具有佐劑。
TW097118119A 2007-07-03 2008-05-16 豬水腫病疫苗 TWI456061B (zh)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2007174919 2007-07-03

Publications (2)

Publication Number Publication Date
TW200914611A TW200914611A (en) 2009-04-01
TWI456061B true TWI456061B (zh) 2014-10-11

Family

ID=40225903

Family Applications (1)

Application Number Title Priority Date Filing Date
TW097118119A TWI456061B (zh) 2007-07-03 2008-05-16 豬水腫病疫苗

Country Status (10)

Country Link
US (1) US9080180B2 (zh)
EP (1) EP2169054B1 (zh)
JP (1) JP5279089B2 (zh)
KR (1) KR20100044813A (zh)
CN (1) CN101868540B (zh)
AR (1) AR067417A1 (zh)
DK (1) DK2169054T3 (zh)
ES (1) ES2564685T3 (zh)
TW (1) TWI456061B (zh)
WO (1) WO2009004842A1 (zh)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2287300B1 (en) 2008-05-02 2018-06-27 Idemitsu Kosan Co., Ltd. Bacterial toxin vaccine
JP5641205B2 (ja) * 2010-07-13 2014-12-17 出光興産株式会社 糖鎖修飾が抑制された改変Ｓｔｘ２ｅタンパク質
US9787725B2 (en) 2011-01-21 2017-10-10 Qualcomm Incorporated User input back channel for wireless displays
CN102580071A (zh) * 2012-02-28 2012-07-18 青岛易邦生物工程有限公司 仔猪水肿病毒素亚单位灭活疫苗的制备方法
BR112016012036A2 (pt) 2013-11-26 2018-05-15 Idemitsu Kosan Co., Ltd. prevenção da diarréia pela escherichia coli
EP3075388A4 (en) 2013-11-26 2017-04-26 Idemitsu Kosan Co., Ltd Vaccine against colibacillosis
CN106574260A (zh) * 2014-08-08 2017-04-19 出光兴产株式会社 猪繁殖与呼吸障碍综合征防治剂
KR20160133120A (ko) 2015-05-12 2016-11-22 (주)애드바이오텍 IgY 함유 돼지부종병 예방용 사료 또는 사료 첨가제
CN107625960B (zh) * 2017-09-22 2020-10-02 江苏省农业科学院 一种通用型猪大肠杆菌病疫苗及其制备方法
KR102103013B1 (ko) * 2019-10-30 2020-04-21 전북대학교산학협력단 Stx2eA-C-말단 단편-Stx2eB-오량체 재조합 단백질； 및 F4를 발현하는 장관독소형 대장균 및 F18을 발현하는 장관독소형 대장균 사균체 혼합 항원을 포함하는 돼지의 병원성 대장균증의 예방 또는 치료용 백신 조성물

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Publication number Publication date
EP2169054A4 (en) 2010-06-02
WO2009004842A1 (ja) 2009-01-08
US9080180B2 (en) 2015-07-14
KR20100044813A (ko) 2010-04-30
TW200914611A (en) 2009-04-01
ES2564685T3 (es) 2016-03-28
CN101868540B (zh) 2014-11-26
DK2169054T3 (en) 2016-03-07
EP2169054B1 (en) 2015-12-23
JPWO2009004842A1 (ja) 2010-08-26
AR067417A1 (es) 2009-10-07
EP2169054A1 (en) 2010-03-31
JP5279089B2 (ja) 2013-09-04
CN101868540A (zh) 2010-10-20
US20110002950A1 (en) 2011-01-06

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