TWI448246B - Fungicidal and insecticidal composition - Google Patents

Description

Fungicidal and insecticidal compositions

The present invention relates to fungicidal and insecticidal compositions, and more particularly to the fungicidal and insecticidal compositions to be applied to nursery boxes, and also to other plant growth-promoting activities. As a component of secondary effects.

A method for cultivating rice seedlings in a seedling box, including treating seedlings with long-lasting insecticides and fungicides during their sowing period, which has been widely used and popular throughout Japan as a labor saving and rice cultivation reduction The cost-effective way is to allow for reduced insect/plant disease control work as rice grows in the field.

The individual active ingredients contained in the composition of the present invention, although it is used alone for treating seeded seeds, under normal conditions, it does not affect the growth of rice, depending on conditions, for example, irrigation (usually called It is "pool nursing", abnormal high temperature, low temperature, drought, etc., which may cause delay in root growth.

A composition containing 3,4-dichloro-N-(2-cyanophenyl)isothiazol-5-carboxyguanamine as a fungicide, and a known insecticidal mixture is known ( WO 2005/009131).

The present inventors have found that the compositions shown below exhibit excellent efficacy. Controls the growth of rice insect/plant diseases transplanted into rice fields, and accelerates the growth of roots that are short during seedling growth, and allows stable growth of good roots of rice seedlings, even under unusual conditions, and is the invention purpose.

Thus, the present invention provides a fungicidal and insecticidal composition comprising the following combination: (a) 3,4-dichloro-N-(2-cyanophenyl)isothiazol-5-carboxamide (after Briefly referred to as isotianil, and (b) fipronil or ethiprole.

The present invention also provides a method for controlling insect/plant diseases after transplanting rice seedlings into a paddy field, comprising: seeding rice seeds in a seedling box and applying the above-mentioned composition to seeds before covering the soil, and After the rice seedlings are transplanted to the paddy field, the method for controlling the insect/plant disease comprises: mixing the seed bed soil of the seedling box or seeding the rice with the same composition as described above.

According to the present invention, prior to the filing of this application, none of the publications explicitly states that (a) isotianil and (b) fipronil or ethiprole are included. The composition of the composition, and the effect of the specific organisms of the composition, so far, in WO 2005/009131, fipronil and ethiprole have been disclosed, only in the general formula, A long list can be mentioned as a possible partner for the compound (A) as a mixture, and in addition, these mixtures are only disclosed in WO 2005/009131 and are considered to have excellent fungicidal activity.

Surprisingly, the fungicidal and insecticidal compositions of the present invention are The composition type comprises (a) isotianil and (b) fipronil or ethiprole, which has excellent efficacy in controlling rice insects and is compared to no application. The root growth of the rice seedlings of the composition exhibits the activity of promoting plant growth, providing excellent root growth of the rice seedlings, and as a result, the growth period of the seedlings is shortened and under abnormal conditions, the rooting of the rice seedlings can be stabilized. Growing up, then, the compositions of the present invention are clearly advantageous and have both control for insect/plant disease and productivity during intervention of shortened seedling growth.

The present invention also provides a composition comprising, in addition to the combination of the two components a) and (b) above, (c) at least one selected from the group consisting of imidacloprid, thiacloprid, and ketone Ding (clothianidin), dinotefuran, thiamethoxam, spinosad, thifluzamide, furametpyr, oressastrobin, and BYF14182 Substances, which are also clearly advantageous and have both insect/plant disease control and the above mentioned productivity.

The component (a) of isotianil according to the present invention is a plant-disease controlling agent disclosed in Japanese Patent Publication (KOHYO) No. 2001-522840, and the above-mentioned component (b) of Fenpu Nepal (fipronil,) and ethiprole (ethiprole) and said component (c) except orysastrobin (orysastrobin) and BYF14182, is disclosed in the ^{Pesticide Manual (13 th Ed.,} published by BCPC, 2003), orysastrobin The oressastrobin is a compound disclosed in German Patent Publication No. 19539324, which is a compound disclosed in WO 2006/092291.

In the combined composition of the present invention, the mixing ratio of the active ingredients should not be Limiting, and may vary over a relatively wide range depending on the particular compound to be mixed, the insect/plant disease to be expected, and the like, and generally, the ratio of mixing may be, for example, for each weight (a Isotianil, using 0.02-3 parts by weight of (b) fipronil or ethiprole, preferably 0.05-2.5 parts by weight of component (b); When component (c) is also contained, (c) may be used in the range of 0.5 to 2 parts by weight, preferably 1 to 1.5 parts by weight, per part by weight of (a) isotianil.

According to another mixing ratio of the present invention, (b) parts by weight of (b) fipronil or ethiprole may be used per part by weight of (a) isotianil, Preferably, the order is: 0.02, 0.05, 0.075, 0.1, 0.15, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8 1.9, 2, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9, 3.

When component (c) is also contained, according to the present invention, the weight ratio of component (c) which can be used per part by weight of (a) isotianil is preferably 0.5, 0.6 in order. , 0.7, 0.8, 0.9, 1, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2.

In the use of the composition of the combination of the present invention, insect/plant disease control after transplanting rice seedlings into paddy fields can be suitably achieved by a method including sowing rice seeds to a seedling box and applying the composition to the seeds, Cover it with soil, or a method, including mixing for breeding The soil of the seed bed of the seedling box or the soil covered with the composition.

Exemplary insect/plant diseases for the purposes of the combined compositions of the present invention include, but are not limited to, the following: rice insects: Hemiptera organisms: Nephotettix cincticeps , Nilaparvata lugens , Laodelphax striatellus (Fallen), Whitebacked Fly ( Sogatella ƒurciƒera ) (Horvath), etc.: Coleoptera: Oulema oryzae (Kuwayama), rice water weevil (Lissorhoptrus oryzophilus Kuschel), and the like; Lepidoptera (Lepidoptera) biological: insect stem borer (Chilo suppressalis) (Walker), rice leafroller (Cnaphalocrocis medinalis) (Guenee), Parnara guttata (Pamara guttata) ( Bremex et Grey), etc.; and rice diseases: Pyricularia oryzae , Pellicularia sasakii , Cercospora janseana , Rhizoctonia oryzae , Gaeumannomyces graminis (Gaeumannomyces graminis var. graminis), Tilletia (Neovossia barclayana), brown spot fungus (Bipolaris oryzae), Pythium Genus (Pythium sp.), Rhizoctonia solani (Rhizoctonia solani), rice seedlings corruption bacteria genera (Achlya sp.) And southern blight fungus (Sclerotium rolfsii), small scoop Sclerotinia (Sclerotium oryzae), brown spot fungus ( Bipolaris oryzae ), Entyloma oryzae , Rhizoctonia solani , Xanthomonas campestris pv. oryzae , and the like.

According to the invention, all plants and plant parts can be treated, meaning all plants and plant groups, for example, desired and unwanted wild plants, plant cultivars and plant varieties (whether or not plant species) Or a plant breeder's rights protector), cultivars and plant varieties are those that can be grown, derived from traditional breeding and breeding methods, by one or more biotechnologies [eg, using double haploids (double Haploids), protoplast fusion, random and direct mutations, molecular or genetic markers, or enhanced or supplemented by methods of bioengineering and genetic engineering; plant parts refer to all parts and organs of plants on the surface and underground. For example, bamboo shoots, leaves, flower buds and roots, for example, leaves, needles, stems, shoots, calyx, fruit bodies, fruits, seeds and roots, bulbs and underground stems, crops and nutritious and reproductive materials, for example, cuttings, Bulbs, underground stems, stolons and seeds also belong to the plant part.

Among the plants to be protected according to the present invention, mention may be made of the main field crops such as corn, soybean, cotton, rapeseed, for example, Brassica napus (for example, canola), Brassica rapa , B. juncea (for example, mustard). And Brassica carinata , rice, wheat, sugar beets, sugar cane, oats, rye, barley, millet, triticale, flax, grapes and various fruits and various vegetables, for example, rose (eg, pome fruit) For example, apples and pears, but also include stone fruits, such as almonds, cherries, apricot kernels and peaches, berry fruits such as strawberries, Ribesioidae sp., Juglandaceae sp., and Betulaceae sp. .), Anacardiaceae sp., Fagaceae sp., Moraceae sp., Oleaceae sp., Actinidaceae sp., Lauraceae Sp.), Musaceae sp. (eg, banana trees and plantings), Rubiaceae sp. (eg, coffee), Theaceae sp., Sterculiceae sp., Rutaceae sp. (for example, lemon, Citrus and grapefruit), Solanaceae sp. (eg, tomato, potato, sweet pepper, eggplant), Liliaceae sp., Compositiae sp. (eg, lettuce, artichoke, and chicory (eg, lettuce, artichoke, and chicory) Chicory) - includes root chicory, endive or common chicory, Umbelliferae sp. (eg, carrot, parsley, celery, and celeriac (celeriac) )), Cucurbitaceae sp. (for example, cucumber - including pickling cucumber, squash, watermelon, gourds and melons), Alliaceae sp. (eg, onions and leeks), Cruciferae sp. (eg, cabbage, red cabbage, broccoli, cauliflower, brussel sprouts, pak choi, Indigo (kohlrabi), radish, wasabi, cress, Shandong cabbage), Leguminosae sp. (for example, peanuts, peas and beans - for example, climbing beans and broad beans), Polygonaceae (Chenopodiaceae sp.) (eg, beet (mangold), spinach beet (spinach beet), spinach (spinach), beetroot), malvaceae (eg, okra (okra)), asparagus (Asparagaceae) (eg, asparagus), horticultural and forestry crops, ornamental plants, and genetically modified homologs of these crops Homologues.

The treatment method according to the invention can be used to treat genetically modified organisms (GMOs), such as plants or seeds; genetically modified plants (or plants of the transgenic genes) in which the heterologous gene has been stably A plant that integrates into its genome, a gene that expresses "heterologous" primarily refers to a gene that is provided or assembled outside the plant, and when introduced into the nucleus, chloroplastic or mitochondria (mitochondrial) genome by expressing an interesting protein or polypeptide or by down-regulating or suppressing other genes (classes) already present in the plant (using, for example, antisense technology, co-suppression techniques or RNA interference-RNAi-technology, which can be obtained with new or improved agronomic or other transformed plants, a heterogeneous gene located in the genome, also known as a transgenic gene, A gene is defined by its particular location within the plant genome and is referred to as a transformation or transgenic event.

Depending on the plant variety or plant cultivar, their distribution and growth conditions (soil, climate, vegetative growth, nutrients), the treatment according to the invention may also result in a superadditive ("co-multiplying") effect, thus for example Reduced application rate and/or augmented activity spectrum and/or enhance activity of active compounds and compositions which can be used according to the invention, better plant growth, higher temperature or low temperature resistance, more drought tolerance or moisture or soil Salt content, increased flowering, easier harvesting, accelerated ripening, higher yield, larger fruit, higher plants, greener leaves, earlier flowering, better quality of harvest and / or higher nutritional value, higher sugar concentration in the fruit, harvest Better storage stability and/or processability of the product may exceed the actual expected effect.

At certain application rates, the active compound combinations according to the invention also have a potentiating effect in plants, and therefore they are also suitable for mobilizing the plant's defense system against attacks by unwanted microorganisms, which may be One of the reasons for the enhanced activity of the composition according to the invention, for example, against fungi, plant-fortified (resistance-inducing) substances, in the context, in the context of the meaning of those substances or combinations of substances, which are capable of Stimulating the plant's defense system such that after it is subsequently inoculated with unwanted microorganisms, the treated plants exhibit a substantial degree of resistance to these microorganisms, in the case of the present invention, unwanted microorganisms are understood to mean insects /plant disease , whereby the substance according to the invention can be applied to protect plants, which can be used against the attack of the above-mentioned pathogens during a period after the plants are treated, the effective period of such protection being usually after treatment of the plants with the active compound, It is from 1 to 10 days, preferably from 1 to 7 days.

Plants and plant cultivars suitably treated according to the invention, including all plants, which are genetic material having a special advantage in participating in these plants (whether by breeding and/or biotechnology) Acquired).

Plants and plant cultivars which are also suitably treated according to the invention are those which are resistant to one or more organisms, ie the plants exhibit a better defense against animal and microbial pests, for example against nematodes , insects, mites, phytopathogenic fungi, bacteria, viruses and/or viroids.

Plants and plant cultivars which may also be treated according to the invention are those plants which are resistant to one or more bio-pressures, and the biological resistance conditions may include, for example, drought, low temperature ridges, exposure to high temperatures Osmotic pressure, flooding, increased soil salinity, increased mineral content, exposure to ozone, high light, limited availability of nitrogen nutrients, limited availability of phosphorus nutrients, shadow avoidance.

Plants and plant cultivars which can also be treated according to the invention are those plants which are characterized by increased yield, resulting in increased yield of plants due to, for example, improved plant physiology, growth and development, for example, water use efficiency, water Retention effectiveness, improved nitrogen use, enhanced carbon assimilation, improved photosynthesis, increased germination efficiency and accelerated maturation; yields can be further improved by improved plant structure (under stress and pressure-free conditions), including , but not limited to, early flowering, flowering control for hybrid seed production, seedling robustness, plant size, number and distance of internodes, root growth, seed size, fruit size, pod size, number of pods or ears, The number of seeds in each pod or ear, seed quality, increased seed filling, reduced seed dissipation, reduced pod rupture and lodging resistance; in addition, yield characteristics include: seed composition, for example, carbohydrates Content, protein content, oil content and composition, nutritional value, anti-nutrition compound reduction, change Good processability and better storage stability.

Plants which can be treated according to the invention are mixed plants which exhibit the characteristics of heterosis or hybrid vieor, which generally result in higher yield, more vigorous, healthier and resistant against biological and non-biotic Physical stress, such plants are typically obtained by crossing a natural male-sterile parental line (maternal line) with another born male-infertile parental line (parent); the hybrid seed is typically self-male Sterile plants are harvested and sold to growers, and sometimes male sterile plants (eg, in the case of corn) can be produced by detasseling, ie, mechanical removal of male genital functions (or male flowers), However, more typically, male sterility lines result in determinants in the plant genome, in which case and in particular when the seed is a desired product obtained from a self-mixed plant, it is typical The ground is used to ensure that male fertility can be fully restored in mixed plants, which can be achieved by ensuring that the male paternal has appropriate fertility restorer genes, which can be genetically responsible for male-sterility Male fertility is restored in mixed plants with determinants; genetic determinants of male sterility may also be located in the cytoplasm, and examples of cytoplasmic male sterility (CMS) are described in For example, in the Brassica species (WO 92/05251, WO 95/09910, WO 98/27806, WO 05/002324, WO 06/021972 and US 6,229,072), however, the genetic determinants for male sterility are also It may be located within the nuclear genome, and male sterile plants may also be obtained by plant biotechnology methods (eg, genetic engineering methods). A particularly useful method for obtaining male-sterile plants is disclosed in WO 89/10396, for example, a ribonuclease, for example, a barnase, a taperum cell selectively expressed in stamens, and a ribozyme inhibitor expressed in a tapetum cell For example, barstar is restored (for example, WO 91/02069).

A plant or plant cultivar (obtained from a plant biotechnology method such as genetic engineering) which can be treated according to the invention is a plant which is tolerant to herbicides, ie is made to tolerate one or more herbicides Plants such plants can be obtained from genetic transformation, or by selecting plants that contain mutations (eg, herbicide tolerance).

The herbicide-tolerant plant is, for example, a plant that is resistant to glyphosate, i.e., a plant that is rendered tolerant to the herbicide galanthom or a salt thereof; the plant can be made resistant in different ways. The herbicide Jiaphos, for example, is obtained from a plant which can be transformed with a gene encoding the enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS). Examples of the EPSPS-like gene are the AroA gene of the Salmonella typhimurium bacterium (variant CT7) (comai et al., 1983, Science 221, 370-371), and the CP4 gene of Agrobacterium sp. (Barry et al., 1992, Curr. Topics Plant Physiol. 7, 139-145), a gene encoding a petunia EPSPS (Shah et al., 1986, Science 233, 478-481), a tomato EPSPS (Gasser et al., 1988, J. Biol. Chem. 263, 4280-4289), or an Eleusine EPSPS (WO 01/66704); also a mutated EPSPS, as disclosed, for example, in EP 0837944, WO 00/ 66746, WO 00/66747 or W002/26995; plants that tolerate glyphosate can also be coded by performance The gene for the oxidative-reductase enzyme is obtained, as disclosed in U.S. Patent Nos. 5,776,760 and 5,463,175; plants resistant to galaxin can also be obtained by expressing a gene encoding a gamma-transferase enzyme, for example, It is described in WO 02/36782, WO 03/092360, WO 05/012515 and WO 07/024782; plants that tolerate glyphosate can also be selected by naturally occurring-existing gene mutations Plants are obtained, for example, from WO 01/024615 or WO 03/013226.

Other herbicide-tolerant plants are, for example, plants which are resistant to inhibition of the glutamine synthase enzyme (for example, bialaphos, phosphinothricin or glufosinate). Plants can be obtained from a glutamine synthase enzyme that exhibits an enzyme detoxifying herbicide or a resistance to inhibition; such an effective detoxifying enzyme is a coding solid. An enzyme of phosphinothricin acetyltransferase (for example, a bar or pat protein derived from Streptomyces species; exhibiting an exogenous phosphinothricin acetyltransferase) Examples of plants are disclosed in, for example, U.S. Patent Nos. 5,561,236, 5,648,477, 5,646,024, 5,273,894, 5,637,489, 5,276,268, 5,739,082, 5,908,810, and 7,112,665.

Another herbicide-tolerant plant is also a plant that is resistant to the herbicide-inhibiting enzyme hydroxyphenylpyruvated ioxygenase (HPPD), and HPPD is a catalytic para-hydroxyphenylpyruvate ( HPP) is an enzyme that is transformed into a response to Homogentisate; plants that tolerate HPPD-inhibitors can encode a naturally occurring resistant HPPD enzyme gene, or a genetically modified HPPD enzyme that has been transformed, Revealed in WO 96/38567, WO 99/24585 and WO 99/24586; tolerant HPPD-inhibitors are also available: by the formation of certain enzymes to form homogentisate regardless of the original HPPD enzyme is inhibited by HPPD- Transgenic plants of the gene inhibited by such agents, such plants and genes are disclosed in WO 99/34008 and WO 02/36787; plants resistant to HPPD inhibitors, in addition to encoding HPPD-resistant enzymes, may also be Transgenic plants having a gene encoding an enzyme of prephenate dehydrogenase are improved, as disclosed in WO 2004/024928.

Further herbicide-tolerant plants are plants which are resistant to acetaminophen lactate synthase (ALS) inhibitors, and known ALS-inhibitors include, for example, sulfonyl urea, imidazolinone, triazolopyrimidine Classes, pyrimidinyloxy(thio)benzoates, and/or sulfonylaminocarbonyltriazolinone herbicides; different mutations in ALS enzymes (also known as acetamidine hydroxyacid synthase) , AHAS), also known to be resistant to different herbicides and a small group of herbicides, for example, revealed by Tranel and Wright (2002, Weed Science 50: 700-712), also disclosed in the United States Patent Nos. 5,605,011, 5,378,824, 5,141,870, and 5,013,659; plants resistant to sulfonylurea and plants resistant to imidazolinone are disclosed in U.S. Patent Nos. 5,605,011, 5,013,659, 5,141,870, 5,767,361, 5,731,180, 5,304,732, 4,761,373, 5,331,107, 5, 928, 937, and 5, 378, 824, and in the international publication WO 96/33270; other imidazolinone-inducing plants are also disclosed, for example, in WO 2004/040012, WO 2004/106529, WO 2005/020673, WO 2005/093093, WO 2006/007373, WO 2006/015376, WO 2006/024351 And WO 2006/060634; in addition, plants which are resistant to sulfhydryl urea and imidazolinone are also disclosed, for example, in WO 07/024782.

Other plants that are tolerant to imidazolinone and/or sulfonyl urea may be derived from induced mutations, selected from cell culture in the presence of a herbicide, or subjected to mutation breeding, for example, soy, WO 97/ in U.S. Patent 5,084,082. The rice of 41,218, the bee of US Patent 5,773,702 and WO99/057965, the lettuce of US Patent 5,198,599, or the sunflower of WO 01/065922.

Plants or plant cultivars (by plant biotechnology methods, such as genetic engineering) which are also treated according to the invention, are plants which are tolerant to insects, i.e., are made to certain targets. Insect-resistant plants, such plants can be made by gene-transformation, or by selecting plants that contain such insect-resistant variants.

The so-called "insect-resistant transgenic plant", including any plant containing at least one transgene, contains a coding sequence for preparation: (1) a species from Bacillus thuringiensis An insect-killing crystalline protein, or an insecticidal protein thereof, for example, a crystal protein listed by Crickmore et al. (1998, Microbiology and Molecular Biology Reviews, 62: 807-813), by Crickmore et al. (2005) Bacillus thuringiensis toxin nomenclature, online updated at: http://www.lifesci.sussex.ac.uk/Home/Neil_Crickmore/ Bt/), or part of the pesticide , for example, a Cry protein Cry1Ab, Cry1Ac, Cry1F, Cry2Ab, Cry3Aa, or Cry3Bb protein or an insecticidal portion thereof; or (2) a crystal protein from Bacillus thuringiensis or a portion thereof, Two kinds of insecticidal properties in the presence of crystal proteins from Suribacter or a part thereof, for example, binary toxins composed of Cry34 and Cry35 crystal proteins (Moel Lenbeck et al. 2001, Nat. Biotechnol. 19: 668-72: Schnepf et al. 2006, Applied Environm. Microbiol. 71.1765-1774); or (3) a mixed insecticidal protein, containing from Suli A part of a different insecticidal crystal protein of Bacillus thuringiensis , for example, a mixture of the above proteins 1) and a mixture of the above 2), for example, Cry1A.105 protein produced by corn MON98034 (WO 2007) /027777); or (4) a protein of any of the above A1) to A3), some of which, in particular, 1 to 10 amino acids are substituted with another amino acid to achieve a higher killing of the target insect species Insect activity, and/or expansion of insect species that can be affected, and/or during cloning or transformation, due to alterations introduced into the encoded DNA, such as the Cry3Bb1 protein in the corn project MON863 or MON88017, or Cry3A in the corn project MIR604 protein; (5) an insecticidal secreted from Bacillus thuringiensis, Bacillus (Bacillus thuringiensis) or Bacillus cereus (Bacillus cereus) protein, or a portion thereof insecticidal, insecticidal proteins such as plant (VIP), listed at: http://www.lifesci.sussex.ac.uk/home/Neil_Crickmore/Bt/vip.html, for example, proteins from the VIP3Aa protein class; or (6) from S. vivax ( Bacillus thuringiensis ) or a secreted protein of Bacillus cereus having insecticidal properties in the presence of a second secreted protein from S. cerevisiae or Bacillus cereus, such as by VIP1A And a binary toxin composed of a VIP2A protein (WO 94/21795); or (7) a mixed insecticidal protein comprising Bacillus thuringiensis or Bacillus cereus from different sources. a secreted protein, such as a mixed protein of the above 1) or a mixed protein of the above 2); or (8) any one of the above 1) to 3), some of which, in particular, 1 to 10 amine groups The acid is replaced by another amino acid to achieve higher insecticidal activity against the target insect, and/or to expand the affected target insect species, and/or to change the introduced encoded DNA when cloning or transformation ( Still coding an insecticide on the one hand Protein), for example, VIP3Aa protein in cotton in the project COT102.

Of course, an insect-tolerant transgenic plant referred to herein also includes any plant comprising a combination of genes encoding any of the above-described classes 1 to 8; in a specific embodiment, the insect-tolerant plant contains More than one transgenic gene encoding any of the above 1 to 8 proteins When used against different target insect species, to spread the affected insect species, or by using different insecticidal proteins, different modes of action for the same species of insects, for example, binding to different receptors of insects The binding position is used to delay the development of insect resistance to plants.

Plants or plant cultivars (obtained from genetically engineered plant biotechnology methods) which are also treated according to the invention are plants which are resistant to abiotic stresses, such plants being obtainable from genetic transformation, Or particularly useful stress-tolerant plants by selecting plants containing mutant factors (eg, stress resistance) include: a. containing a poly(ADP-ribose) polymerase (PARP) gene that reduces plant cells or plants. Plants for the expression and/or activity of the transgenic genes are disclosed, for example, in WO 00/04173 or EP 04077984.5 or EP 06009836.5.

b. A plant comprising a transgenic gene which increases the tolerance and/or activity of a PARG-encoded gene in a plant or plant cell, for example, as disclosed in WO 2004/090140.

c. A plant containing a plant-function enzyme that enhances stress tolerance for compiling a plant-function enzyme of the salvage adenine dinucleotide synthesis pathway, including: nicotinamide (nicotinamidase), nicotinate phosphoribosyltransferase, nicotinic acid mononucleotide adenyl transferase, nicotinamide adenine dinucleotide synthase or nicotine 醯Aminophosphoryltransferase (nicotine amide) Phosphorybosyltransferase), as disclosed in EP 04077624.7 or WO 2006/133827 or PCT/EP07/002433.

Plants or plant cultivars (derived from plant biotechnology methods such as genetic engineering) that are also treated according to the invention may exhibit altered production, quality and/or storage stability and/or harvest of the harvested product. The altered nature of a particular component of a product, for example: 1) the physico-chemical properties of a genetically modified plant that synthesizes a modified starch, particularly amylose content or amylose/amylopectin ( Amylose/amylopectin) ratio, degree of branching, average chain length, side chain distribution, viscous behavior, gel strength, starch granule size and/or starch granule morphology, etc., compared to wild-type plant cells or plants The starch is modified to make it more suitable for a particular application, said transgenic plant synthesizing a modified starch, as disclosed in, for example, EP 0571427, WO 95/04826, EP 0719338, WO 96/ 15248, WO 96/19581, WO 96/27674, WO 97/11188, WO 97/26362, WO 97/32985, WO 97/42328, WO 97/44472, WO 97/45545, WO 98/27212, WO 98/ 40503, WO 99/58688, WO 99/58690, WO 99/58654, WO 00/08 184, WO 00/08185, WO 00/08175, WO 00/28052, WO 00/77229, WO 01/12782, WO 01/12826, WO 02/101059, WO 03/071860, WO 2004/056999, WO 2005/ 030942, WO 2005/030941, WO 2005/095632, WO 2005/095617, WO 2005/095619, WO 2005/095618, WO 2005/123927, WO 2006/018319, WO 2006/103107, WO 2006/108702, WO 2007/009823, WO 00/22140, WO 2006/063862, WO 2006/072603, WO 02/034923, EP 06090134.5, EP 06090228.5, EP 06090227.7, EP 07090007.1, EP 07090009.7, WO 01/14569, WO 02/79410, WO 03/33540, WO 2004/078983, WO 01/19975, WO 95/26407, WO 96/34968, WO 98/20145, WO 99/12950, WO 99/66050, WO 99/53072, US 6734341, WO 00/11192, WO 98/22604, WO 98/32326, WO 01/98509, WO 01/ 98509, WO 2005/002359, US 5824790, US 6013861, WO 94/04693, WO 94/09144, WO 94/11520, WO 95/35026-WO 97/20936, 2) relative to non-genetically modified wild type A plant, a plant which synthesizes a non-starch carbohydrate polymer or a gene for synthesizing a non-starch carbohydrate polymer having an altered property, an example of a plant: a plant which produces polyfructose, in particular an inulin. And a levan-type, as disclosed in EP 0663956, WO 96/01904, WO 96/21023, WO 98/39460 and WO 99/24593 Plants which produce alpha-1,4-glucans (glucans), for example, are disclosed in WO 95/31553, US 2002031826, US 6284479, US 5712107, WO 97/47806, WO 97/47807, WO 97/47808 and In WO 00/14249, alpha-1,6-branched alpha-1,4- Plants of dextran, such as those disclosed in WO 00/73422, plants producing alternan, such as those disclosed in WO 00/47727, EP 06077301.7, US 5908975 and EP 0728213, 3) production of hyaluronan Plants, for example, are disclosed in WO 2006/032538, WO 2007/039314, WO 2007/039315, WO 2007/039316, JP 2006304779, and WO 2005/012529.

- Plants of particularly useful transgenic genes that can be treated according to the invention are plants containing a combination of transformation events, or transformational items, and US Department of Agriculture (USDA) US Animal and Plant Health Inspection Service (APHIS), which is the goal of a petition for a non-regulated state, whether or not such a petition is granted or remains unresolved, at any time, this information is readily available from APHIS (4700 River Road Riverdale, MD 20737, USA), for example, from its Internet address (URL http://www.aphis.usda.gov/brs/not_reg.html).

Additional particularly useful plants containing a single transformational item or a combination of transformational items are listed, for example, in a database of regulatory agencies of various countries or regions (see, for example, http://gmomfo .jrc.it/gmp_browse.aspx and http://www.agbios.com/dbase.php).

Other particular transgenic plants include plants containing a transgenic gene in an agriculturally neutral or advantageous position, as described in any of the patent publications listed in Table A.

The compositions of the present invention can be formulated into conventional formulation forms for use in the control of rice insect/plant diseases.

Examples of formulation types may include aerosol solution dispersions, capsule suspensions, cold mist concentrates, hot mist concentrates, coated granules, fine granules, flowable concentrates for treating seeds, ready to use Solution, dust-dyeing powder, emulsifiable concentrate, oil-based emulsion, water-based emulsion, macro-particle, micro-particle, oil-dispersible powder, flowable in oil Concentrates, liquids that can be mixed with oil, froths, pastes, seed coatings with pesticides, suspension concentrates (flowable concentrates), suspension-emulsion concentrates, soluble Concentrates, suspensions, soluble powders, granules, water-soluble granules or lozenges, water-soluble powders for treating seeds, wettable powders, natural and synthetic materials infiltrated with active compounds Particles coated in a polymeric material and coated seeds, as well as ULV-cold and thermal atomization formulations, gases (under pressure), gas generating products, plant rodlets, Powder for treating dry seeds, solution for treating seeds, ultra low capacity (ULV) liquid, ultra low volume (ULY) suspension, water dispersible granules or tablets, water dispersible powders for slurry treatment, and the like.

The formulations according to the invention can be produced according to known methods, which can be produced, for example, by mixing the above-mentioned active ingredient with a solid or liquid carrier or diluent, optionally with a surfactant, a stretching agent Agrochemically acceptable additives or other pharmaceutical adjuvants.

The solid carrier and/or solid diluent used in the formulation according to the invention may, for example, be an ore-based inorganic substance, for example, Clay, kaolin, talc, calcium carbonate, diatomaceous earth, zeolite, bentonite, acid clay, activated earth, American activated clay, montmorillonite, vermiculite, pearlite, pumice, quartz Synthetic granules of quartz (quartz sand), quartzite (calcite), calcite, marble, sepiolite, dolomite, and inorganic and organic powders; (synthetics) (eg, white carbon, such as hydrophilic vermiculite, hydrophobic vermiculite and calcium citrate, alumina, synthetic zeolite, titanium dioxide, etc.); plant organic materials (eg, soy flour, tobacco powder, corn) Cobs, walnut powder, wheat flour, wood chips, starch, crystalline cellulose, paper, sawdust, coconut shells, cornstalks, tobacco rods, etc.); synthetic or natural compounds (eg, xanthonone resin ( Coumarone resin)), petroleum resin, alkyd resin, polyalkylene glycol, ketone resin, ester gum, etc.; wax (for example, carnauba wax) (camauba wax), beeswax, etc.); water soluble Substances (eg, urea, lactose, sucrose, ammonium sulfate, potassium chloride, etc.).

Suitable liquid carriers and/or liquid diluents include, for example, fats and oils, for example, coconut oil, rapeseed oil, corn oil, soybean oil, rice bran oil, and the like; paraffin or naphthoic acid hydrocarbon solvents, For example, gasoline, mineral oil, spindle oil, white oils, normal olefins, isoolefins, naphthalene, etc.; Aromatic hydrocarbon solvents, for example, xylene, alkylbenzene, alkylnaphthalene, etc.; alcohols such as ethanol, benzyl alcohol, isopropanol, cyclohexanol, etc.; polyhydric alcohols, for example, ethylene glycol , diethylene glycol, propylene glycol, polyethylene glycol, etc.; ether-alcohols, for example, ethylene glycol ethyl ether, ethylene glycol phenyl ether, diethylene glycol ethyl ether, diethylene glycol butyl Ethers and the like; ketones, for example, methyl ethyl ketone, diisobutyl ketone, cyclohexanone, acetophenone, isophorone, gamma-butyrolactone, etc.; esters, for example, Fatty acid methyl esters (eg, coconut oil fatty acid methyl ester), dibasic acid methyl esters (eg, dimethyl succinate, dimethyl glytamate, dimethyl diphosphate) Ester, ethyl acetate, amyl acetate, ethylene glycol acetate, diethylene glycol acetate, etc.; ethers, for example, dioxane, tetrahydrofuran, etc.; polar solvents, for example, dimethylformamide , dimethyl acetamide, dimethyl hydrazine, N-alkyl pyrrolone, etc. or water, and the like.

In addition, surfactants, binders, and other adjuvants may be used for emulsifying, dispersing, wetting, spreading, anti-decomposing, and increasing potency of the active ingredient and/or for improving the physical properties of the formulation ( The control of dispersibility, the improvement of flowability, the related antifreeze effect and the rain resistance, etc.), the surfactants that can be used include any type of non-ionic, anionic, cationic and amphoteric surfactants. Although non-ionic and/or anionic surfactants are typically employed; suitable non-ionic surfactants may include, for example, sorbitan fatty acid esters, polyoxyalkylene sorbitan fatty acid esters, sucrose fatty acid esters. Classes, polyoxyalkylene fatty acid esters, polyoxygen Alkyl resin esters, polyoxyalkylene fatty acid diesters, polyoxyalkylene castor oil, polyoxyalkylene hardened castor oil, polyoxyalkylene alkyl ethers, polyoxyalkylene groups Alkyl phenyl ethers, polyoxyalkylene dialkyl phenyl ethers, polyoxyalkylene alkyl phenyl ether-formaldehyde condensates, polyoxyethylene/polyoxypropylene bulk copolymers, alkyl poly Oxyethylene/polyoxypropylene bulk copolymer ethers, alkylphenyl polyoxyethylene/polyoxypropylene bulk copolymer ethers, polyoxyalkylene alkylamines, polyoxyalkylene fatty acid guanamines , polyoxyalkylene benzylphenyl (or phenylphenyl) ethers, polyoxyalkylene styrylphenyl (or phenylphenyl) ethers, polyoxyalkylene ethers - and esters Types of anthrones and fluorinated surfactants and the like.

In addition, suitable anionic surfactants may include, for example, alkyl sulfate salts, polyoxyalkylene alkyl ether sulfate salts, polyoxyalkylene alkylphenyl ether sulfate salts, polyoxygen Alkylene benzyl (or styryl) phenyl (or phenyl phenyl) ether sulfate, polyoxyethylene / polyoxypropylene agglomerate sulfate, olefin (alkane) sulfonic acid Ester salts, alpha olefin sulfonate salts, dialkyl sulfosuccinates, alkyl benzene sulfonate salts, mono- or dialkyl naphthalene sulfonate salts, naphthalene sulfonate / formaldehyde Condensate salts, alkyl diphenyl ether disulfonate salts, lignosulfonates, polyoxyalkylene alkylphenyl ether sulfonate salts, polyoxyalkylene ether sulfo amber Acidic half-esters, fatty acid salts, N-methyl fatty acid sarcosinates, resinates, polyoxyalkylene alkyl ether monophosphates, polyoxyalkylene mono- or dialkyl Phenyl ether phosphates, polyoxyalkylene benzylated (or styrenated) phenyl (or phenylphenyl) ether phosphates, polyoxyethylene / polyoxypropylene bulk polymerization Phosphate salts, Polyacrylates, and so on.

The cationic surfactants used in the present invention include ammonium type surfactants, for example, alkyltrimethylammonium chloride, methyl-polyoxyethylene-alkylammonium chloride, alkyl-N-methyl bromide Pyridinium chloride, mono- or dialkylmethylated chloride, alkyl pentamethylpropylenediamine dichloride, etc.; benzalkonium type surfactant, for example, alkyl An alkyldimethyl benzalkonium chloride, a benzethonium chloride or the like.

The amphoteric surfactant may comprise a surfactant of the betaine type, for example, a dialkyldiaminoethyl inner salt, an alkyl dimethylbenzyl inner salt, and the like.

In addition, exemplary binders may include bentonite, casein, animal glue, starch, dextrin, lignosulfonate, alginate, gum arabic, xanthan gum, carboxymethyl cellulose, methyl fiber. , polyvinyl alcohol, polyvinylpyrrolidone, natural phospholipids, for example, cephalin and lecithin and synthetic phospholipids and the like.

In addition, other adjuvants include, for example, disintegration control agents, antifreeze agents, antiseptic agents, flowability improving agents, suds suppressors, humectants, extenders, and rain-resistant agents. (rain-resistance-conferring agents), colorants, and the like.

The above carriers and surfactants, as well as binders and various adjuvants may be suitably used singly or in combination, depending on the use, the type of the formulation, the stage of administration, and the like.

The total amount of the formulation may contain from 0.1 to 95% by weight, preferably from 0.5 to 90% by weight, of the active ingredient.

The combination composition of the invention may be used as such or in its various formulation versions, for example, in the form of a separate "ready-mix", in the form of a mixed spray mixture, in separate formulations consisting of the individual active compounds, For example, in the "cartridge-mixture" to be used, and in the subsequent application, separate active ingredients are used, ie, the active ingredients are used one after the other in a reasonably short time, for example, hours or days. Preferably, the order in which the compounds (a) and (b) and/or (c) are applied is not critical to the operation of the present invention.

Instance

Test Example 1: Testing the growth and strength of rice seedlings The standard seedling box for planting rice seedlings (30 cm x 60 cm x 3 cm) was filled into about 2.5 liters of artificial nursery soil and seeded with pre-germinated seed kernels. (Variety: Koshi-hikari), immediately after irrigation, uniformly apply 50 g of a granular formulation containing individual active ingredients at a predetermined concentration to each seedling box, and cover with 1 liter of artificially cultivated soil. On the seed, the seedling box is then placed in a warm nursery room and placed at about 30 ° C for 2 days. After the buds are emerged, the box is moved to a plastic greenhouse to grow the seedlings and continuously observe the growth with the naked eye. Three weeks after sowing (the most suitable stage for transplantation), the green stems and roots (the formation of the rice seedlings, especially the roots at the bottom and sides of the box) are observed with the naked eye. In addition, one piece is cut (10 The seedlings of the seedlings are divided into x10 cm) and the breakine limit values are determined to estimate the strength of the seedlings in the nursery box. More specifically, after one of the seedlings of the seedlings is tied, On the other side The mechanical force gauge is slowly stretched, and the value read at the moment of the rupture of the plexiform pad is the breaking limit value (stretching strength); Table 1 shows the average score obtained by the naked eye observation and the experiment. Group, one piece (10 cm x 10 cm) cut from the seedlings of the seedlings, and the average breaking limit measured at each end by triple measurement (6 pieces in total).

The evaluation criteria for visual inspection are: 1 = healthy 2= Root growth has some delay 3=The root growth has a moderate delay and will not adversely affect their actual use. 4 = delay in root growth slightly adversely affects their actual use 5=Severe delays in root growth adversely affect their actual use

Evaluation criteria for the strength of seedlings: (A score of 100 represents the average tensile strength of an untreated seedling mat using a mechanical strength meter) 1=95 or more, 2=90 to less than 95, 3=70 to less than 90, 4=50 to less than 70, 5=less than 50

Test Example 2: Evaluation of insect control effects against Pamara guttata (Bremex et Grey) Method: Once Asian rice ( Oryza sativa ) (variety: Koshi-hikari) was cultured in a standard seedling box At the most appropriate stage of transplantation, they consist of 50 grams per box, 2% (w/w) isotianil and 1% (w/w) fipronil particles. After being treated and slightly watered, the material was immediately transplanted into the paddy field with a rice planter, cultivated and managed according to the regional farming method, and 70 days after the transplant, the 600 stocks in each section were determined. The number of damaged blocks is shown in Table 2.

Test Example 3: Evaluation of the effect of plant disease control against Pyricularia oryzae method Once the Asian rice ( Oryza sativa ) (variety: Koshi-hikari) was cultured in a standard seedling box for the most appropriate transplantation stage They were treated with the same particle composition as in Example 2 in an amount of 50 g/box. After slightly watering, they were immediately transplanted into paddy fields with a rice planter, and cultivated and managed according to regional farming methods. Days, the proportion of damage in the 120 stocks in each section is examined and the results are shown in Table 3.

Formulation Example 1 (microparticles) 85.5 parts by weight of clay particles having a particle size distribution of 0.2-0.7 mm were filled in a rotary vessel type mixer, and 2 parts by weight of isotianil were added during the rotation. And microparticulates were prepared by adding 1 part by weight of fipronil and spraying 10 parts by weight of a liquid diluent, and then adding 1.5 parts by weight of white carbon.

Formulation Example 2 (microparticles) 84.5 parts by weight of clay particles having a particle size distribution of 0.2-0.7 mm were filled in a rotary vessel type mixer, and 2 parts by weight of isotianil were added during rotation. And 1 part by weight of fipronil and 1 part by weight of imidacloprid and sprayed with 10 parts by weight of a liquid diluent, and then 1.5 parts by weight of white carbon, to prepare a microparticle.

Formulation Example 3 (water-dispersible granules) 30 parts by weight of isotianil, 15 parts by weight of fipronil, 15 parts by weight of sodium lignosulfonate, and 15 parts by weight of bentonite Sodium (montmorillonite), 15 parts by weight of smoldering diatomaceous earth and 10 parts by weight of talc, after thorough mixing, adding 5 parts by weight of polyoxyalkylene stilbene phenyl group The water of the sodium ether sulfate was mixed, and the mixture was further kneaded, extruded by a 0.5 mm sieve through an additional extrusion granulator, and dried to obtain a water-dispersible granule.

Claims (8)

A fungicidal and insecticidal composition comprising: (a) 3,4-dichloro-N-(2-cyanophenyl)isothiazol-5-carboxamide, and (b) Fenpney ( a combination of fipronil), and (c) imidacloprid, wherein the component (b) is contained in a range of 0.02-3 parts by weight, and contains the component (c), per part by weight of the component (a). The range is from 0.5 to 2 parts by weight. A method for controlling an insect/plant disease, characterized by applying a composition according to item 1 of the patent application to rice. A method for visualizing plant growth-promoting activity, characterized by administering a composition according to item 1 of the patent application to rice seedlings. The method according to the third aspect of the patent application, characterized in that the plant-growing promoting activity promotes the growth of roots. A use of a composition according to item 1 of the scope of the patent application for controlling insect/plant diseases. A use of a composition according to item 1 of the patent application for the growth-promoting activity of a plant. A method for controlling insect/plant diseases after rice is transplanted to a rice field, comprising: seeding rice seeds in a seedling box, and applying the composition according to item 1 of the patent application to the soil before applying the soil to On the seed. A method for controlling insect/plant diseases after rice is transplanted to a paddy field, comprising: soil for seed bed of seedling box or soil covering rice seed and composition according to item 1 of the patent application scope mixing.