JP2010506866A - Bactericidal insecticidal composition - Google Patents

Abstract

  A bactericidal insecticidal composition comprising a combination of (a) 3,4-dichloro-N- (2-cyanophenyl) isothiazole-5-carboxamide and (b) fipronil or etiprol.

Description

  The present invention relates to a bactericidal insecticidal composition. Specifically, it relates to a fungicidal insecticidal composition applied to a seedling box, and also relates to the composition having a plant growth promoting action as a secondary action.

  In paddy rice box seedlings, the method of treating long-lasting insecticides and fungicides at the time of sowing can be used as an effective means for labor saving and cost reduction of paddy rice cultivation because the number of control during the growing period of Honda can be reduced. Has already been promoted and is already widely practiced in various parts of Japan.

  Each active ingredient contained in the composition of the present invention, when treated alone at the time of sowing under normal cultivation conditions, does not affect the growth of rice, but it is a flooded seedling (commonly called pool raising), abnormally high temperature, Depending on conditions such as low temperature and drying, the growth of the root may be somewhat delayed.

  A mixed composition of the bactericidal compound 3,4-dichloro-N- (2-cyanophenyl) isothiazole-5-carboxamide and a known insecticide is already known (WO 2005/009131).

International Publication No. 2005/009131 Pamphlet

  The present inventors have shown that the combination shown below has an excellent control effect against pests of rice after transplanting to Honda, and promotes root growth to shorten the seedling raising period, even under different conditions. As a result, the present inventors have found that stable root growth of paddy rice seedlings can be achieved and the present invention has been completed.

Thus, the present invention provides (a) 3,4-dichloro-N- (2-cyanophenyl) isothiazole-5-carboxamide (hereinafter abbreviated as isothianyl);
(B) A fungicidal insecticidal composition comprising a combination of fipronil or etiprole is provided.

  The present invention also includes a method for controlling pests after transplanting to Honda, comprising seeding paddy rice seeds in a seedling box and applying the above composition before covering the soil, as well as floor soil of the seedling box or paddy rice seeds. The present invention also provides a method for controlling pests after transplanting to Honda, which comprises mixing the above composition with the soil to be covered.

  According to the present invention, the description specifically identified in the composition comprising the combination of isotianil of (a) and fipronil or etiprole of (b), the specific biological effect of the composition, It is not disclosed in the publication. So far, in WO2005 / 009131 fipronil and etiprol are disclosed in the general formula only as possible mixing partners for compound (A) in the long list of mixing partners. Furthermore, these mixtures are only described in WO 2005/009131 for their very good bactericidal activity.

  Surprisingly, the bactericidal insecticidal composition comprising the combination of (i) isotianil of (a) and fipronil or etiprole of (b) of the present invention exhibits an excellent control effect against rice pests and the composition. Compared to the case where no seedling is applied, the plant growth promotion effect of promoting the growth of roots of rice seedlings can be shown very well. However, stable root growth can be achieved. Therefore, the composition of the present invention is extremely advantageous and useful in terms of both productivity of pest control and shortening of the seedling raising period.

  The present invention further includes, in addition to the combination of the two components (a) and (b) above, the group consisting of imidacloprid, thiacloprid, clothianidin, dinotefuran, thiamethoxam, spinosad, tifluzamide, flametopyr, orisatrobin and BYF14182. A composition comprising at least one selected from the above is also provided, and the composition comprising a combination of the components (a), (b) and (c) is also capable of both pest control and productivity. It is extremely advantageous and useful.

  The isotianil of (a) in the present invention is one of disease control agents disclosed in JP-T-2001-522840, and fipronil and etiprole of (b), and among the above components of (c), Except for BYF14182, these compounds are described in Pesticide Manual (2003 edition, published by BCPC). Orisatrobin is a compound described in German Patent No. 19539324. BYF14182 is a compound described in WO2006 / 092921.

  In the combination composition of the present invention, the mixing ratio of each active ingredient is not limited and can be varied over a relatively wide range depending on the type of active compound to be mixed, the occurrence of pests, etc. it can. In general, the mixing ratio is, for example, 0.02 to 3 parts by weight, preferably 0.05 to 2.5 parts by weight of fipronil or etiprole of (b) with respect to 1 part by weight of isotianil of (a). When the range and (c) component are added, the (c) component can be used in the range of 0.5 to 2 parts by weight, preferably 1 to 1.5 parts by weight.

In accordance with the present invention, a further mixing ratio can be used as part by weight of (b) fipronil or ethiprole per part by weight of (a) isotianil (higher priority in the order given):
0.02, 0.05, 0.075, 0.1, 0.15, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0. 9, 1, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9, 3.

If component (c) is also included, according to the present invention, further mixing ratios as parts by weight of component (c) per part by weight of (a) isothianyl can be used (priority in the order given). Higher):
0.5, 0.6, 0.7, 0.8, 0.9, 1, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2.

  In using the combination composition of the present invention, the seeds are sown in a seedling box, and the composition is applied to the soil before covering the paddy rice seeds or the floor soil of the seedling box or the method of applying the composition before covering the soil. By the method of mixing, pest control after Honda transplantation can be accurately performed.

  Examples of the pests that are controlled by the combination composition of the present invention include the following, but are not limited thereto.

As paddy rice pests: Hemiptera: Nephotettix cinchetics, Japanese planthopper (Nilaparvata lugens), Japanese planthopper (Laodedelfax stratiellas (Fallen)), White-tailed planthopper (Sotaharva).

  Coleoptera: Rice beetle (Oulema oryzae (Kuwayama)), rice weevil (Lissorhoptrus oryzophilus Kuschel), etc.

  Lepidoptera: Nigella cypress (Chiro suppressalis (Walker)), Cnaphalocros medinalis (Guenee), Inetomi (Parnara guttata (Bremex et Grey)) and the like.

  As paddy rice diseases, there are rice blast disease (Piculararia oryzae), coat blight (Pericularia sasakii), Cercospora jansiana, rice red fungus disease (Rhizoctonia oryzae), mincion, and wheat. Barbrayana (Neovossia barclayana), rice sesame leaf blight fungus (Bipolaris oryzae), seedling blight (Pythium sp.), Barley leaf rot fungus (Rhizoctonia solani), cotton fungus (Achlisti white silkworm) , Rice brown sclerotia fungus (Sclerot um oryzae), rice sesame leaf blight (Bipolaris oryzae), black Zhu disease (Entyloma oryzae), grasses leaf blight fungus (Rhizoctonia solani), rice bacterial leaf blight (Xanthomonas campestris pv oryzae), such as.

  According to the invention, all plants and plant parts can be treated. Plants as used herein are all plants and plant populations (whether protected or not protected by the rights of plant varieties or plant breeders, including desirable and undesirable wild plants, varieties and plant varieties). ). Varieties and plant varieties are supplemented or supplemented by one or more biotechnological methods, such as by doubling haploids, protoplast fusion, random and directed mutagenesis, using molecular or genetic markers or by biotechnology and genetic engineering methods It can be a plant obtained by conventional breeding and breeding methods that can be done. Plant parts include all aboveground and underground parts and organs of plants, such as shoots, leaves, flowers and roots (examples include leaves, needles, stems, stems, flowers, fruit bodies, fruits and And seeds, roots, tubers and rhizomes). Harvested plants and vegetative and reproductive propagation materials such as cuttings, corms, rhizomes, toothpicks and seeds also belong to the plant part.

  Among the plants that can be protected by the method according to the invention, the main crops include corn, soybean, cotton, oilseed rape, such as Brassica napus (for example canola), turnip (Brassica rapa), mustard ( Various fruits of rice, wheat, rye, barley, millet, triticale, flax, vine and various plant taxa, such as B. juncea (e.g. mustard) and Abyssinia mustard (Brassica carinata) Vegetables such as Rosaceae sp. (Eg, seed fruits such as apples and pears, and stone fruits such as apricots, cherries, almonds and peaches, berries such as strawberries), Ribesioidae sp., Walnut family (Juglandaceae sp.), Birch family (Betulaceae sp.), Urushi family (Anacardiaceae sp.), Beech family (Fagaceae sp.), Mulberry family (Spaceae, Sp. Family) Oleaceae sp.), Actinidaceae sp., Lauraceae sp., Musaceae sp. (Eg, banana trees and planting), Rubiaceae sp. (Eg, coffee), camellia Theaceae (Steraceae sp.), Sterculaeae sp., Rutaceae sp. (Eg, lemon, orange and gray) Solaaceae sp. (Eg, tomato, potato, pepper, eggplant), Lilyaceae sp., Compositae sp. (Eg, lettuce, artichoke and chicory-root chicory, endive or Chicory), umbelliferae sp. (Eg carrot, parsley, celery and root celery), cucurbitaceae sp. (Eg cucumber-pickle cucumber, pumpkin, watermelon, cucumber and melon) ), Alliaceae sp. (Eg, onions and leaks), Brassicaceae (Cruciferae sp.) (Eg, white cabbage, purple cabbage, broccoli, cauliflower, brussels sprouts) , Bok choy, kohlrabi, radish, horseradish, cress, Chinese cabbage), legumes (Leguminosae sp. ) (E.g. peanuts, peas and legumes-such as bean beans and faba beans), Chenopodiaceae sp. (E.g. chard, spinach beet, spinach, beetroot), malvaceae (e.g. malvaceae) ), Asparagaceae (eg asparagus); horticulture and forest crops; ornamental plants; and genetically modified homologues of these crops.

  In the treatment of genetically modified organisms (GMO), for example plants or seeds, the treatment method according to the invention can be used. A genetically modified plant (or transgenic plant) is a plant in which a heterologous gene is stably integrated into the genome. The expression of a “heterologous” gene basically means a gene that has been provided or constructed from outside the plant, and when introduced into the nucleus, the chloroplast or mitochondrial genome is the protein of interest or Transformed by expressing the polypeptide or by down-regulating or suppressing other genes present in the plant (eg, using antisense technology, co-suppression technology or RNA interference-RNAi technology) Renew or improve the agricultural or other characteristics of the plant. A heterologous gene located in the genome is also called a transgene. A transgene defined by its specific location in the plant genome is called a transformation or transgenic event.

  Depending on the plant species or plant varieties, their location and growth conditions (soil, climate, vegetation period, nutrients), the treatment according to the invention can also result in superadditive (“synergistic”) effects. Thus, for example, by exceeding the expected effect, reducing the spread rate and / or expanding the activity spectrum and / or improving the activity of the active compounds and compositions that can be used according to the invention, Better growth, improve tolerance to high or low temperatures, improve tolerance to dryness or water or soil salt content, improve flowering performance, promote harvest, accelerate maturity, improve yield , Increase fruit size, lengthen plant height, darken leaves green, speed flowering, improve quality and / or nutritional value of harvested products, increase sugar concentration in fruits, stable storage of harvested products And / or processability can be improved.

  At a certain application rate, the active compound combinations according to the invention can also have a strengthening effect in plants. They are therefore also suitable for moving the plant's defense system from attack by unwanted microorganisms. If necessary, this can be one of the reasons for enhancing the activity of the combination according to the invention, for example against fungi. In the context of the present invention, plant strengthening (tolerance-inducing) substances are produced in such a way that, when undesired microorganisms are continuously inoculated, the treated plants exhibit a sufficient degree of resistance to these microorganisms. It should be understood as meaning a substance or a combination of substances that can stimulate the defense system. In this case, undesirable microorganisms are to be understood as meaning pests. In this way, the substances according to the invention can be used to protect plants from attack by the above mentioned pathogens within a certain period of time after treatment. The period during which the protection is effective usually ranges from 1 to 10 days, preferably from 1 to 7 days, after treatment of the plant with the active compound.

  Preferably, the plants and plant varieties to be treated according to the invention are all advantageous to these plants and all plants with genetic material that give useful characteristics (those obtained by breeding and / or biotechnological techniques) ) Is included.

  The plants and plant varieties to be treated according to the invention also preferably are resistant to one or more biological stresses, ie the plants are resistant to disease animals and fungi, for example nematodes, insects, mites, Higher protection against phytopathogenic bacteria, bacteria, viruses and / or viroids.

  Plants and plant varieties that can also preferably be treated according to the invention are plants that are resistant to one or more abiotic stresses. Non-biological stress conditions include, for example, dryness, low temperature exposure, heat exposure, osmotic stress, flooding, high salt soil, high mineral exposure, ozone exposure, high light exposure, and limited use of nitrogen nutrients May include restricted use of phosphorus nutrients, shade avoidance.

  Plants and plant varieties that can also be treated according to the present invention are plants characterized by improved recovery characteristics. Improvement in recovery in this plant means, for example, promotion of plant physiology, growth and development, for example, water use efficiency, efficient water retention, improvement of nitrogen use, improvement of carbon assimilation, improvement of photosynthesis, improvement of germination efficiency And the result of promoting maturity. Harvesting further includes but is not limited to early blooming, flowering regulation for hybrid seed production, seedling vitality, plant size, internode number and distance, root growth, seed size, fruit size, Includes pod size, number of pods or spikes, number of seeds per pod or spike, seed mass, improved ripening, reduced seed dispersal, reduced dehiscence and lodging resistance (under stress and non-stress conditions Can be affected by improvements in plant structure. Additional harvest characteristics include seed composition, eg, carbohydrate content, protein content, fat content and composition, nutritional value, reduced anti-nutritive compounds, improved processability and improved storage stability.

  Plants that can be treated according to the present invention are hybrid plants that already express the characteristics of hybrid or hybrid stresses, which generally result in higher yields, are stronger, have better health and improved resistance to biological and abiotic stresses. . Such plants are usually produced by crossing an inbred male sterile parent strain (female parent) with another inbred male fertile parent strain (male parent). Hybrid seed is usually recovered from male sterile plants and sold to farmers. Male sterile plants can sometimes be produced (eg in corn) by removing the tufts (ie, mechanically removing the male genital organs (or male flowers)), but more commonly, male sterility. Sex is the result of genetic determination in the plant genome. In that case, and especially when the seed is the desired product to be harvested from the hybrid plant, it is generally useful to ensure that male fertility is fully restored in the hybrid plant. This can be done by ensuring that the male parent has an appropriate fertility recovery gene that can restore male fertility in a hybrid plant containing genetic determinants involved in male sterility. it can. Genetic determinants for male sterility can be located in the cytoplasm. Examples of cytoplasmic male sterility (CMS) are described, for example, in Brassica species (WO 92/05251, WO 95/09910, WO 98/27806, WO 05/002324, WO 06/021972 and US 6,229,072). ing. However, genetic determinants for male sterility can also be located in the nuclear genome. Male sterile plants can also be obtained by plant biotechnology methods such as genetic engineering. A particularly useful means of obtaining male sterile plants is described, for example, in WO 89/10396, where a ribonuclease such as barnase is selectively expressed in, for example, stamen carpet tissue cells. Next, fertility can be restored by the expression of ribonuclease inhibitors such as balsters in carpet tissue cells (eg WO 91/02069).

  Plants or plant varieties (obtained by plant biotechnology methods such as genetic engineering) that can be treated according to the present invention are herbicide-tolerant plants, i.e. plants that are made tolerant to one or more certain herbicides. It is. Such plants can be obtained either by genetic transformation or by selection of plants containing mutations that confer such herbicide tolerance.

  A herbicide-tolerant plant is, for example, a glyphosate-tolerant plant, that is, a plant adapted to be resistant to the herbicide glyphosate or a salt thereof. Plants can be made resistant to glyphosate through various means. For example, glyphosate-tolerant plants can be obtained by transforming plants with a gene encoding the enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS). Examples of such EPSPS genes include the Salmonella typhimurium AroA gene (mutant CT7) (Comai et al., 1983, Science 221, 370-371), and the CP4 gene of the Agrobacterium sp. 1992, Curr. Topics Plant Physiol. 7, 139-145), a gene encoding Petunia EPSPS (Shah et al., 1986, Science 233, 478-481), tomato EPSPS (Gasser et al., 1988, J. Biol. Chem). 263, 4280-4289) or Eleusine EPSPS (WO 01/66704). It can also be a mutant EPSPS as described for example in EP0837944, WO00 / 66746, WO00 / 66747 or WO02 / 26995. Glyphosate-tolerant plants can also be obtained by expressing a gene encoding the glyphosate oxide-reductase enzyme as described in US Pat. Nos. 5,776,760 and 5,463,175. For example, as described in WO02 / 36782, WO03 / 092360, WO05 / 012515 and WO07 / 024782, glyphosate-tolerant plants can be obtained by expressing a gene encoding a glyphosate acetyltransferase enzyme. For example, as described in WO01 / 024615 or WO03 / 013226, glyphosate-tolerant plants can be obtained by selecting plants that contain natural mutations of the above genes.

  Other herbicide-tolerant plants are plants that have been made resistant to herbicides that inhibit the enzyme glutamine synthase, such as bialaphos, phosphinotricin or glufosinate, for example. Such plants can be obtained by expressing an enzyme that detoxifies the herbicide or a mutant glutamine synthase enzyme that is resistant to inhibition. One such effective detoxifying enzyme is an enzyme that encodes a phosphinothricin acetyltransferase (such as a bar or pat protein from Streptomyces species). Plants expressing exogenous phosphinothricin acetyltransferase include, for example, US Pat. Nos. 5,561,236; 5,648,477; 5,646,024; 5,273,894. No. 5,637,489; No. 5,276,268; No. 5,739,082; No. 5,908,810 and No. 7,112,665. .

  Further herbicide-tolerant plants are also plants that have been rendered tolerant to herbicides that inhibit the enzyme hydroxyphenylpyruvate dioxygenase (HPPD). Hydroxyphenylpyruvate dioxygenase is an enzyme that catalyzes a reaction in which para-hydroxyphenylpyruvate (HPP) is converted to homogentisic acid. Transforming plants resistant to HPPD inhibitors with a gene encoding a naturally resistant HPPD enzyme or a gene encoding a mutant HPPD enzyme as described in WO 96/38567, WO 99/24585 and WO 99/24586 Can do. Resistance to HPPD inhibitors can also be obtained by transforming plants with genes encoding certain enzymes that allow the formation of homogentisic acid despite the inhibition of native HPPD enzymes by HPPD inhibitors. . Such plants and genes are described in WO99 / 34008 and WO02 / 36787. As described in WO 2004/024928, in addition to the gene encoding HPPD-resistance, the plant resistance to HPPD inhibitors can also be improved by transforming the plant with a gene encoding the enzyme prefenate dehydrogenase. .

  Still further herbicide tolerant plants are plants that have been rendered tolerant to acetolactate synthase (ALS) inhibitors. Known ALS-inhibitors include, for example, sulfonylureas, imidazolinones, triazolopyrimidines, primimidinoxy (thio) benzoates and / or sulfonylaminocarbonyltriazolinone herbicides. Various mutations in the ALS enzyme (also known as acetohydroxyacid synthase, AHAS) are described, for example, in Train and Wright (2002, Weed Science 50: 700-712) and in US Pat. No. 5,605,01. No. 5,378,824, 5,141,870 and 5,013,659 are known to confer resistance to various herbicides and herbicide groups. It has been. Production of sulfonylurea and imidazolinone resistant plants is described in US Pat. Nos. 5,605,011; 5,013,659; 5,141,870; 5,767,361; No. 5,731,180; No. 5,304,732; No. 4,761,373; No. 5,331,107; No. 5,928,937 and No. 5,378 , 824; and International Publication No. WO 96/33270. Other imidazolinone-tolerant plants are also described, for example, in WO2004 / 040012, WO2004 / 106529, WO2005 / 020673, WO2005 / 093093, WO2006 / 007373, WO2006 / 015376, WO2006 / 024351 and WO2006 / 060634. Further sulfonylurea- and imidazolinone-tolerant plants are also described, for example in WO07 / 024782.

  For example, US Pat. No. 5,084,082 for soybeans, WO 97/41218 for rice, US Pat. Nos. 5,773,702 and WO 99/057965 for sugar beets, US Pat. Imidazolinone and / or sulfonylurea by induced mutagenesis, selection in cell culture in the presence of herbicides or mutation breeding as described in WO 01/065922 for sunflowers, 5,198,599 or sunflower Other plants that are resistant can be obtained.

  Plants or plant varieties that can also be treated according to the present invention (obtained by plant biotechnology methods such as genetic engineering) are insect resistant transgenic plants, ie plants that have been made resistant to attack by certain target insects. is there. Such plants can be obtained by transformation or by selection of plants containing mutations that confer such insect resistance.

As used herein, an “insect-resistant transgenic plant” includes any plant containing at least one transgene that includes a coding sequence that encodes:
1) Insecticidal crystal protein derived from Bacillus thuringiensis or an insecticidal portion thereof, such as the insecticidal crystal protein listed in Crickmore et al. (1998, Microbiology and Molecular Biology Reviews, 62: 807-813). In the cis-toxin nomenclature, updated by Crickmore et al. (2005), online, http://www.lifeci.sussex.ac.uk/Home/Neil_Crickmore/Bt/) or its insecticidal part, eg Cry protein class Cry1Ab, Cry1Ac, Cry1F, Cry2Ab, Cry3Aa or Cry3Bb protein or insecticide thereof Or 2) a binary toxin composed of a crystal protein from Bacillus thuringiensis or a part thereof that is insecticidal in the presence of a second other crystal protein from Bacillus thuringiensis or a Cry34 and Cry35 crystal protein ( (part of binary toxin) (Moellenbeck et al., 2001. Nat. Biotechnol. 19: 668-72; Schnepf et al., 2006, Applied Environm. Microbiol. 71.1765-1774); or 3) from Bacillus thuringiensis Hybrid insecticidal proteins comprising a portion of various insecticidal crystal proteins, such as the protein hybrids of 1) above or the protein hybrids of 2) above, such as corn Cry1A produced by Cosi event MON98034 (WO2007 / 027777). 105 protein; or 4) any one of the proteins A1) to A3) above (to obtain higher insecticidal activity against the target insect species and / or to expand the range of affected target insect species, and / or Or, due to changes introduced into the coding DNA during cloning or transformation, some, especially 1 to 10, amino acids have been replaced by another amino acid)), eg Cry3Bb1 protein in corn event MON863 or MON88017 or Cry3A protein in corn event MIR604;
5) Insecticidal protein derived from Bacillus thuringiensis or Bacillus cereus or its insecticidal part, for example http: // www. lifesci. sussex. ac. uk / home / Neil_Crickmore / Bt / vip. plant insecticidal (VIP) proteins listed in html, eg VIP3Aa protein class; or 6) Bacillus thuringiensis or B. Binary toxins composed of secreted proteins from Bacillus thuringiensis or Bacillus cereus that are insecticidal in the presence of a second secreted protein from Cereus, such as VIP1A and VIP2A proteins (WO94) / 21795); or 7) a hybrid insecticidal protein comprising portions derived from different secreted proteins from Bacillus thuringiensis or Bacillus cereus, eg, a hybrid of the protein of 1) above or a hybrid of the protein of 2) above; or 8) In order to obtain a higher insecticidal activity against the target insect species and / or to expand the range of affected target insect species, any one of the above 1) to 3) And / or due to changes introduced into the coding DNA during cloning or transformation, some, especially 1 to 10, amino acids have been replaced by another amino acid (while still encoding an insecticidal protein). )), VIP3Aa protein of cotton event COT102.

  Needless to say, insect-resistant transgenic plants, as used herein, also include any plant that contains a combination of genes that encode any one of the above classes 1-8 proteins. In certain embodiments, insect-resistant plants use insecticides to broaden the range of affected target insect species when using different proteins directed to different target insect species, or against the same target insect species. In order to delay the development of insect resistance to plants by using different proteins with different but different modes of action (eg binding to different receptor binding sites in insects) It contains a plurality of transgenes encoding any one protein from 1 to 8.

Plants or plant varieties that can also be treated according to the present invention (obtained by plant biotechnology methods such as genetic engineering) are resistant to abiotic stress. Such plants can be obtained by transformation or by selection of plants containing mutations that confer such stress tolerance. Particularly useful stress tolerant plants include:
a. Plants containing a transgene capable of reducing the expression and / or activity of the poly (ADP-ribose) polymerase (PARP) gene in plant cells or plants as described in WO00 / 04173 or EP040777984.5 or EP06009836.5 .
b. A plant containing a stress tolerance promoting transgene capable of reducing the expression and / or activity of a PARG-encoding gene in a plant or plant cell, as described in WO 2004/090140, for example.
c. Including nicotinamidase, nicotinate phosphoribosyltransferase, nicotinic acid mononucleotide adenyltransferase, nicotinamide adenine dinucleotide synthetase or nicotinamide phosphoribosyltransferase, as described for example in EP 04077624.7 or WO 2006/133828 or PCT / EP07 / 002433 A plant comprising a stress tolerance promoting transgene encoding a functional enzyme in a plant of the nicotinamide adenine dinucleotide salvage synthesis pathway.

Plants or plant varieties that can also be treated according to the present invention (obtained by plant biotechnology methods such as genetic engineering) are the quantitative, quality and / or storage stability changes of the harvested product and / or the specifics of the harvested product. Indicates a change in the properties of an ingredient: for example
1) transgenic plants that synthesize modified starch (its physicochemical properties, in particular amylose content or amylose / amylopectin ratio, degree of branching, average chain length, side chain distribution, viscous behavior, gel strength, starch particle size and And / or starch particle morphology has changed compared to synthetic starch in wild type plant cells or plants to make it more suitable for special applications). The transgenic plants that synthesize modified starch are, for example, EP 0571427, WO95 / 04826, EP0719338, WO96 / 15248, WO96 / 19581, WO96 / 27274, WO97 / 11188, WO97 / 26362, WO97 / 32985, WO97 / 42328, WO97 / 44472, WO97 / 45545, WO98 / 27212, WO98 / 40503, WO99 / 58688, WO99 / 58690, WO99 / 58654, WO00 / 08184, WO00 / 08185, WO00 / 08175, WO00 / 28052, WO00 / 77229, WO01 / 12782, WO01 / 12826, WO02 / 101059, WO03 / 071860, WO2004 / 056999, WO2 05/030942, WO2005 / 030941, WO2005 / 095632, WO2005 / 095617, WO2005 / 095619, WO2005 / 095618, WO2005 / 123927, WO2006 / 018319, WO2006 / 103107, WO2006 / 108702, WO2007 / 009823, WO00 / 22140, WO00 / 22140 063862, WO2006 / 076023, WO02 / 034923, EP06090134.5, EP0609028.5, EP06090227.7, EP0707097.1, EP070900099.7, WO01 / 14569, WO02 / 79410, WO03 / 078983, WO01 / 079993, WO01 / 19975 WO95 / 264 7, WO96 / 34968, WO98 / 20145, WO99 / 12950, WO99 / 66050, WO99 / 53072, US67334341, WO00 / 11192, WO98 / 22604, WO98 / 32326, WO01 / 98509, WO01 / 98509, WO2005 / 002359, US5824790, US 6013861, WO 94/04693, WO 94/09144, WO 94/11520, WO 95/35026, WO 97/20936.
2) A transgenic plant that synthesizes a non-starch carbohydrate polymer or synthesizes a non-starch carbohydrate polymer with altered properties without genetic modification compared to a wild-type plant. Examples include plants producing polyfructose (especially inulin and levan type) such as disclosed in EP 0 663 356, WO 96/01904, WO 96/21023, WO 98/39460 and WO 99/24593, WO 95/31553, US2002031826, US6284479, Plants producing α1,4 glucan as disclosed in US 5712107, WO 97/47806, WO 97/47807, WO 97/47808 and WO 00/14249, α-1,6 branched α-as disclosed in WO 00/73422 Plants producing 1,4-glucan, producing alternans as disclosed in WO 00/47727, EP06077301.7, US 5908975 and EP 0728213 Such as plants.
3) Examples include transgenic plants that produce hyaluronan as disclosed in WO2006 / 032538, WO2007 / 039314, WO2007 / 039315, WO2007 / 039316, JP2006304779 and WO2005 / 012529.

  Particularly useful transgenic plants that can be treated according to the present invention are unregulated against the Department of Agriculture (USDA) Animal and Plant Health Inspection Service (APHIS) in the United States, whether such petition is granted or pending. A plant containing a transformation event or a combination of transformation events that is the subject of a petition for a condition. This information is always readily available from APHIS (4700 River Road Riverdale, MD 20737, USA), for example at its Internet site (URL http://www.aphis.usda.gov/brs/not_reg.html). .

  Additional particularly useful plants containing one transformation event or a combination of transformation events are listed, for example, in databases from various national or local regulatory authorities (see, for example, http: //gmoinfo.jrc. see it / gmp_browse.aspx and http://www.agbios.com/dbase.php).

  More particularly, transgenic plants include plants that are agronomically neutral or contain the transgene in a beneficial position as described in any of the patent publications listed in Table A.

  When the combination composition of the present invention is used for controlling paddy rice pests, it can be converted into a usual preparation form.

  Examples of dosage forms include aerosol dispensers, capsule suspensions, cold spray concentrates, hot spray concentrates, encapsulated granules, fine granules, flowable concentrates for seed treatment, prepared solutions, powders, emulsifiable concentrates. concentrate), oil-in-water emulsion, water-in-oil emulsion, macro granule, fine granule, oil-dispersible powder, oil-miscible flowable concentrate, oil-miscible liquid, foam, paste, pesticide-coated seed, suspending agent (floorable) Agent), suspension-emulsion concentrate, soluble concentrate, suspension, soluble powder, granule, granule or tablet wettable powder, water solvent for seed treatment, hydration Agents, natural and synthetic materials impregnated with active compounds, microencapsulation in polymeric materials and seed coatings, and ULV-cooled Spray formulation, gas (under pressure), gas generating product, plant rodlet, dry seed treatment powder, seed treatment solution, ultra-low volume (ULV) liquid, ultra-low volume (ULV) suspension, granule or tablet Wettable powders, powdered wettable powders for slurry processing and the like may be included.

  These preparations can be prepared by methods known per se. For example, each of the above active ingredients is mixed with a solid or liquid carrier or diluent, if necessary, a surfactant, a bulking agent, an agriculturally acceptable additive or other formulation adjuvant. The preparation according to the invention can be prepared by:

Examples of the solid carrier and / or solid diluent that can be used in the formulation of the composition of the present invention include inorganic materials based on ore (for example, clay, kaolin, talc, calcium carbonate, diatomaceous earth, zeolite, bentonite, acidic clay, Active clays, attapulgous clay, montmorillonite, vermiculite, perlite, pumice, cinnabar, aragonite, calcite, marble, cuffstone, dolomite) and synthetic granules of inorganic and organic powders;
Synthetic products (white carbon, such as hydrophilic silica, hydrophobic silica and calcium silicate, aluminum oxide, synthetic zeolite, titanium dioxide, etc.);
Plant organic matter such as corn cob and tobacco stem from soy flour, tobacco powder, corn cob flour, walnut flour, wheat flour, wood flour, starch, crystalline cellulose, paper, sawdust, coconuts;
Synthetic or natural polymer compounds such as coumarone resin, petroleum resin, alkyd resin, polyalkylene glycol, ketone resin, ester gum;
Waxes such as carnauba wax and beeswax;
Water-soluble substances such as urea, lactose, sucrose, ammonium sulfate and potassium chloride can be exemplified.

Suitable liquid carriers and / or liquid diluents include, for example, oils and fats such as coconut oil, rapeseed oil, corn oil, soybean oil, rice bran oil;
Paraffinic or naphthenic hydrocarbon solvents such as kerosene, mineral oil, spindle oil, white oil, normal paraffin, isoparaffin, naphthene;
Aromatic hydrocarbon solvents such as xylene, alkylbenzene, alkylnaphthalene;
Alcohols such as ethanol, benzyl alcohol, isopropanol, cyclohexanol;
Polyhydric alcohols such as ethylene glycol, diethylene glycol, propylene glycol, polyethylene glycol;
Ether alcohols such as ethylene glycol ethyl ether, ethylene glycol phenyl ether, diethylene glycol ethyl ether, diethylene glycol butyl ether;
Ketones such as methyl ethyl ketone, diisobutyl ketone, cyclohexanone, acetophenone, isophorone, γ-butyrolactone;
Fatty acid methyl esters such as coconut oil fatty acid methyl ester, dimethyl succinate, dibasic acid methyl ester such as dimethyl glutamate and dimethyl adipate, esters such as ethyl acetate, amyl acetate, ethylene glycol acetate, diethylene glycol acetate;
Ethers such as dioxane and tetrahydrofuran;
Examples include polar solvents such as dimethylformamide, dimethylacetamide, dimethylsulfoxide, N-alkylpyrrolidone, water, and the like.

  In addition, for the purpose of emulsifying, dispersing, wetting, spreading, preventing decomposition, enhancing efficacy and / or improving the physical properties of the formulation (disintegration control, fluidity improvement, freezing prevention, provision of rain resistance, etc.) Surfactants, binders and other adjuvants can be used. As the surfactant that can be used, any type of nonionic, anionic, cationic and zwitterionic compounds can be used, but usually nonionic and / or anionic compounds are used. used. Suitable nonionic surfactants include, for example, sorbitan fatty acid esters, polyoxyalkylene sorbitan fatty acid esters, sucrose fatty acid esters; polyoxyalkylene fatty acid esters, polyoxyalkylene resin acid esters, polyoxyalkylene fatty acid diesters; polyoxy Alkylene castor oil, polyoxyalkylene hydrogenated castor oil; polyoxyalkylene alkyl ether; polyoxyalkylene alkyl phenyl ether, polyoxyalkylene dialkyl phenyl ether, polyoxyalkylene alkyl phenyl ether formalin condensate; polyoxyethylene / polyoxypropylene block polymer, alkyl Polyoxyethylene / polyoxypropylene block polymer ether, alkylphenyl polyoxye Polyoxyalkylene alkylamine, polyoxyalkylene fatty acid amide; polyoxyalkylene benzylphenyl (or phenylphenyl) ether, polyoxyalkylene styrylphenyl (or phenylphenyl) ether; polyoxyalkylene ether and Examples include ester type silicon and fluorine-based surfactants.

  Suitable anionic surfactants include, for example, alkyl sulfate salts, polyoxyalkylene alkyl ether sulfate salts, polyoxyalkylene alkyl phenyl ether sulfate salts, polyoxyalkylene benzyl (or styryl) phenyl (or phenylphenyl). Ether sulfate salt, polyoxyethylene / polyoxypropylene block polymer sulfate salt; paraffin (alkane) sulfonate salt, alpha olefin sulfonate salt, dialkyl sulfosuccinate salt, alkylbenzene sulfonate salt, mono or dialkyl naphthalene sulfonate salt, naphthalene sulfonate / formalin condensation Salt, alkyl diphenyl ether disulfonate, lignin sulfonate, polyoxyalkyl Alkyl phenyl ether sulfonate salt, polyoxyalkylene ether sulfosuccinic acid half ester salt; fatty acid salt, N-methyl fatty acid sarcosinate, resin acid salt; polyoxyalkylene alkyl ether phosphate salt, polyoxyalkylene mono- or dialkyl phenyl ether phosphate salt, polyoxy Examples thereof include alkylene benzyl (or styryl) phenyl (or phenylphenyl) ether phosphate salt, polyoxyethylene / polyoxypropylene block polymer phosphate salt; polyacrylate, polycarboxylate and the like.

  Cationic surfactants include alkyl trimethyl ammonium chloride, methyl polyoxyethylene alkyl alkyl ammonium chloride, alkyl N-methyl pyridinium bromide, mono- or dialkyl methylated ammonium chloride, alkyl pentamethylpropylenediamine dichloride, and other ammonium types; alkyl Examples thereof include benzalkonium types such as dimethylbenzalkonium chloride and benzethonium chloride.

  Examples of amphoteric surfactants include betaine types such as dialkyldiaminoethylbetaine and alkyldimethylbenzylbetaine.

  As binders, bentonite, casein, gelatin, starch, dextrin, lignin sulfonate, alginate, gum arabic, xanthan gum, carboxymethyl cellulose, methyl cellulose, polyvinyl alcohol, polyvinyl pyrrolidone, natural phospholipids (for example, cephalin and lecithin) and Examples thereof include synthetic phospholipids.

  Examples of other adjuvants include disintegrating preparation agents, antifreezing agents, preservatives, fluidity improvers, antifoaming agents, moisturizing agents, spreading agents, rain resistance imparting agents, and coloring agents.

  The above carriers, surfactants, binders and various adjuvants can be appropriately used alone or in combination depending on the purpose in consideration of the dosage form of the preparation, application scenes, and the like.

  In general, the preparation can be contained at a concentration of 0.1 to 95% by weight, preferably 0.5 to 90% by weight in total of each active ingredient.

  The combination compositions of the present invention, in use, are used as such or in the form of their various formulations, for example in a combined spray mixture consisting of individual formulations of one active compound, in one “prepared” form. Such as “tank mixing” at the time), and when applied continuously, can be used in combination with a single active ingredient (ie, sequentially in a reasonably short period (hours or days)). Preferably, the order in which compounds (a) and (b) and / or (c) are applied is not essential for practicing the present invention.

Examples Test example 1: Strength and growth test of paddy rice seedling Method About 2.5 liters of artificial soil for seedling seedling was spread in a standard seedling box for paddy rice (30 cm x 60 cm x 3 cm), and the sprouting seed pod (cultivar Koshihikari) was sown. Immediately after irrigation, 50 g of each active ingredient granular material prepared in advance so as to have a predetermined concentration was uniformly dispersed per box and covered with 1 l of artificial soil for raising seedlings. Thereafter, the seedling box was placed in a warm germination device and held at about 30 degrees for 2 days. After emergence, the seedlings were transferred to a nursery greenhouse, and the degree of seedling growth was continuously observed with the naked eye. Three weeks after sowing (suitable time for transplantation), the foliage and roots (so-called mat formation degree, especially the amount of rooting on the bottom of the box and the side) were visually observed. Further, a portion (10 cm × 10 cm) of the box seedling was cut out, and the strength of the box seedling was evaluated by measuring the breaking limit value of the seedling. That is, one of the seedlings was fixed, and the other seedling was gradually pulled by using a mechanical force gauge, and the value when the seedling broke was measured as the breaking limit value (= tensile strength). Evaluation by macroscopic observation is the average value of 3 repetitions, and the seeds for fracture limit value measurement (10 cm x 10 cm) are shown in Table 1 for the average results of measuring 6 pieces in total at 2 repetitions. Evaluation of visual observation Standard:
1 = Healthy 2 = Slightly delayed root growth 3 = Slightly delayed root growth but no practical problem 4 = Slightly problematic root growth 5 = Practical problem Root growth delay Evaluation criteria for box seedling strength:
Assuming that the average value of tensile strength by mechanical force gauge in the untreated section is 100,
1 = 95 or more 2 = 90 or more and less than 95 3 = 70 or more and less than 90 4 = 50 or more and less than 70 5 = less than 50

Test example 2
Test for control effect on rice beetles Method 50g of a granule composition containing 2% (w / w) isothianyl and 1% fipronil (w / w) in rice (variety Koshihikari) in a suitable time for transplantation cultivated in a standard nursery box for paddy rice After spraying, lightly irrigated and immediately transplanted using a rice planter. Cultivation management was carried out according to local practice, and the number of damaged strains was counted for 600 strains in each ward 70 days after transplantation.

  The results are shown in Table 2.

Test example 3
Test method for controlling rice blast disease Method 50g of the same granule composition as in Test Example 2 is sprayed on rice (variety Hitomebore) suitable for transplantation cultivated in a standard rice seedling box for paddy rice. And transplanted. Cultivation management was carried out according to local practices, and the lesion area rate was examined for 120 strains in each ward 72 days after transplantation. The results are shown in Table 3.

Formulation Example 1 (fine granules)
Put 85.5 parts by weight of clay mineral particles having a particle size distribution of 0.2-0.7 mm in a rotating container mixer, and spray 10 parts by weight of liquid diluent and 2 parts by weight of isotianil and 1 part by weight of fipronil under rotation. Then, 1.5 parts by weight of white carbon is added to make a fine granule.

Formulation Example 2 (fine granules)
84.5 parts by weight of clay mineral particles having a particle size distribution of 0.2-0.7 mm are placed in a rotating container mixer. Under rotation, 10 parts by weight of liquid diluent and 2 parts by weight of isothianyl, 1 part by weight of fipronil, imidacloprid 1 part by weight is sprayed and 1.5 parts by weight of white carbon is added to form a fine granule.

Formulation Example 3 (granular wettable powder)
30 parts by weight of isotianil, 15 parts by weight of fipronil, 15 parts by weight of sodium lignin sulfonate, 15 parts by weight of sodium bentonite (montmorillonite), 15 parts by weight of calcined diatomaceous earth powder and 10 parts by weight of talc are mixed thoroughly, and this is polyoxyalkylene After adding and kneading water containing 5 parts by weight of tristyrylphenyl ether sulfate sodium salt, the mixture is extruded with an extrusion granulator equipped with a 0.5 mm screen and dried to obtain a granular wettable powder.

Claims (12)

(A) 3,4-dichloro-N- (2-cyanophenyl) isothiazole-5-carboxamide;
(B) A bactericidal insecticidal composition comprising a combination of fipronil or etiprol.   The composition according to claim 1, further comprising (c) at least one member (c) selected from the group consisting of imidacloprid, thiacloprid, clothianidin, dinotefuran, thiamethoxam, spinosad, tifluzamide, furametopir, orisatrobin and BYF14182.   The composition according to claim 1 or 2, which has a plant growth promoting action as a secondary action.   The composition according to claim 3, wherein the plant growth promoting action is a rooting promoting action.   The composition according to claim 1, wherein the component (b) is blended within a range of 0.02 to 3 parts by weight with respect to 1 part by weight of the component (a).   The composition according to claim 1, 2 or 5, wherein the component (c) is blended in the range of 0.5 to 2 parts by weight with respect to 1 part by weight of the component (a).   A method for controlling pests, comprising applying the composition according to claim 1 or 2 to paddy rice.   A method for exerting a plant growth promoting action, which comprises applying the composition according to claim 1 or 2 to rice seedlings.   Use of the composition according to claim 1 or 2 for controlling pests.   Use of the composition according to claim 1 or 2 for exerting a plant growth promoting action.   A method for controlling pests after transplanting to Honda, comprising seeding paddy rice seeds in a nursery box and applying the composition according to claim 1 or 2 to the seeds before covering.   A method for controlling pests after transplanting to Honda, comprising mixing the composition of claim 1 or 2 with floor soil of a seedling box or soil covering rice seeds.