CN103562158B

CN103562158B - The method for improving plant quality

Info

Publication number: CN103562158B
Application number: CN201180068862.2A
Authority: CN
Inventors: 里诺·米格尔·迪亚斯; 马丁·沃佩尔; 大嶋明久; 杨晓军; 若昂·爱德华多·维拉迈尔
Original assignee: Bayer Intellectual Property GmbH
Current assignee: Bayer CropScience AG
Priority date: 2010-12-31
Filing date: 2011-12-27
Publication date: 2018-04-27
Anticipated expiration: 2031-12-27
Also published as: CN103562158A

Abstract

The present invention relates to a kind of method for improving plant quality, and the described method includes use the processing crop of the micronutrient containing active ingredient and/or crop growing loci or the region and/or the propagulum that are intended to long-term cropping for improving plant quality dosage.

Description

The method for improving plant quality

Technical field

The present invention relates to a kind of new method for improving plant quality, the described method includes to improve containing for plant quality dosage There are the active ingredient processing crop and/or crop growing loci or the region and/or the crop that are intended to long-term cropping of micronutrient Brood body.

Background technology

Zinc is the required micronutrient of the normal growth of plant and the mankind, development and health.At present it is known that large area agriculture Industry land used zinc-deficiency, causing the nutritional quality of crop yield and cereal crops seriously reduces.In many countries, zinc deficiency is still within The situation that can not be identified or be underestimated, is not handled.Therefore, lacked there is an urgent need to understand in these countries and solve zinc Weary situation, to promote crop production and human health.Zinc has crop under the conditions of various stress factors such as arid, high temperature and salt More preferable tolerance is also particularly important.

To applying zinc fertilizer on soil and/or plant leaf blade, to solve the problems, such as crop zinc-deficiency provide one it is simple and efficient Scheme, and add the zinc concentration of food.This strategy prevents the unnecessary loss of grain-production significantly, and helps to change Kind public health.For example, the enrichment of zinc can save up to 48 every year in India in rice and wheat seed, 000 children's Life.For people millions of all over the world, additionally several milligrams of zinc can cause disease or dead and health daily Difference between efficient life.By ensuring that there is crop sufficient zinc to supply, we can by provide significant health, Social and economic benefit helps to solve this global problem (Zinc Fertilizer Brochure 2009, published by theInternational Zinc Association IFA；see also Comparative Quantification/ Global and Regional Burden of Diseases Attributable to Selected Major Risk Factors of Health Risks,Ezzati,Lopez,Rodgers,Murray(eds.),Chapter 5–Zinc Deficiency,WHO,2004)。

It is required that zinc is grown with breeding for the normal health of plant, animals and humans.When the available zinc of plant During supply deficiency, the product quality of crop yield and crop will be weakened.In plant, zinc is made in many important biochemical pathways Key effect is played for the constituent or regulation and control confactor of numerous different enzymes and protein.When supply plant zinc not When sufficient, zinc cannot correctly play its physiological action so as to cause plant growth unfavorable, can cause plant generation is visible stress disease Shape, these symptoms can change and may include with species：(blade becomes chlorosis between arteries and veins between hypoevolutism (height reduction), arteries and veins It is yellow), the bronzed disease of chlorosis blade, (wherein blade exists for small and abnormal shape blade and/or blade hypoevolutism and rosettes Screw thread is formed on the stem of shortening).The so-called stealth for not having clearly visible symptom lacks the loss that can cause 20% plant products.Zinc Stealthy shortage can cause the significant economic loss of peasant caused by shortage soil.Therefore, soil testing or crop analysis are passed through Identify that zinc deficiency soil is necessary as early as possible.Come once identifying, the soil of zinc deficiency can be handled easily by zinc fertilizer Sufficient zinc is provided to crop to supply, wherein zinc sulfate is fertilizer (cf.Zinc in Soils most widely used so far and Crop Nutrition by B.J.Alloway,2^ndEd.Brussels,Paris,2008)。

Furthermore it is known that comparing untreated grapevine seedling, the grapevine seedling handled with zinc sulfate or Difar has There are yield and the growth of obvious bigger (referring to ISHS Acta Horticulturae (gardening) 1987,199,157-161). And also it has been shown that influence (the cf. not due to yield of prevention zinc-deficiency in barleyEPPOBull., 1981,11,277- 285).The micronutrients such as copper, manganese and zinc can be by being fed in plant containing this kind of micronutrient fungicide.However, this Type depending on the complex compound that the metal and other molecules are formed, if absorb efficiently or inefficiently micronutrient.Therefore, It has been found that compared with the zinc in zineb, the zinc in some ziram preparations is not easy to be absorbed (cf.University Of Florida, IFAS Extension, HS1159,2009).

In addition, it has been disclosed together with insecticide to plant, the composition of more specifically potato delivering zinc oxide (referring to state Border Patent publication No 95/20874).

The content of the invention

The present invention provides a kind of method for the quality for improving plant, is characterized in, with improving, containing for plant quality dosage is micro- The active ingredient processing crop of amount nutrient and/or the region of plant growth or the region of intentional long-term cropping and/or plant are numerous Body is grown, this active ingredient preferably has Fungicidally active concurrently at the same time.

Preferably, the active ingredient containing micronutrient be selected from containing at least one metal ion activity into Point, the metal ion is selected from the group：Zinc, manganese, molybdenum, iron and copper or micronutrient boron.

It is highly preferred that these active ingredients be selected from a kind of such as Propineb of the compound containing zinc, more foster mycin Z (zinc salt), Zineb, ziram, thiadiazoles zinc (zinc thiodazole), zinc naphthenate and Mancozeb (also comprising manganese)；Or it is selected from Compound containing manganese such as maneb, Carbatene and mancopper (also comprising copper)；Or selected from such as good fortune of the compound containing iron U.S. iron；Or selected from the compound containing copper such as bordeaux mixture, COPPER OXYCHLORIDE 37,5, ternary copper sulphate, cupric oxide, cupric octoate, hydrogen Cupric oxide, copper 8-hydroxyquinolinate and copper naphthenate.

It is highly preferred that the active ingredient is selected from Propineb and Mancozeb.

Most preferably, the active ingredient is Propineb.

It is known that the active ingredient mentioned above containing micronutrient has fungicidal properties, and be used to control Make various plant diseases (cf.The Pesticide Manual, 15th Edition 2009).

Term " plant quality " (quality of plant) is defined as crop and/or the situation of its product, and the situation passes through It is several individually or the aspect that is combined with each other determines, such as yield (such as biomass increase, the increasing of valuable components content Add and/or the improvement of some component contents or composition) and plant growing way (plant vigor) (such as the improvement of plant growth And/or blade is greener).

One index of plant quality, the index in particular for plant situation are its yield, and " yield " is interpreted as planting Any plant parts or product with economic value of thing production, such as fruit on seed, leaf, root, proper sense, Vegetables, nut, seed, timber (in terms of forestry) are even spent (in terms of gardening and ornamental plant).In addition plant product can Also it can will be further employed and/or process after harvesting.

According to the present invention, " volume increase " of crop refers to compared under equal conditions without using the work containing micronutrient The yield of the same product of the crop production of property component, the product yield of each crop are increased with measurable amount.Increased yield It can especially be characterized by following improved crop characteristic：

Plant weight increase,

Plant height increase,

Zn content increase,

Iron content increase,

Calcium content increase,

The increase of biomass, such as the fresh weight (FW) and/or dry weight (DW) of higher,

The grain yield of higher,

The acidity of higher,

The anthocyanidin content of higher,

Tiller is more,

Blade bigger,

Young sprout growth increase,

* increases (such as soluble protein),

Oil content increase,

Content of starch increase,

Pigment content increase,

Nutrient content increase,

Protein content increase,

Vitamin content increase (such as vitamin B₁、B₂, C and E),

Content of fatty acid increase,

Metabolite content increase,

Carotenoid content increases (such as vitamin A),

The amount increase of essential amino acid,

Improved trophic component,

Improved protein composition,

Improved aliphatic acid composition,

Improved metabolite composition,

Improved carotenoid composition,

Improved sugar composition,

Improved amino acid composition,

The fruit color for improving or optimizing,

Improved leaf color,

The memory capacity of higher,

The machinability of harvested product higher.

An embodiment according to the present invention, compared to each check plant being not handled by, according to it to crop yield Improvement ability can improve at least 5%, 10%, 25%, 50% or even more.

Another is directed to plant quality, and the index especially for crop situation is " plant growing way ".Plant growing way is embodying At several aspects, such as overall visual appearance and growth.The improvement of plant growing way can especially pass through following improved plant characteristic To characterize：

Improved plant vigor,

Improved plant growth,

Improved development of plants,

Improved visual appearance,

Improved plant support (less plant lodging),

Improved sprouting,

The root growth of enhancing and/or more flourishing root system,

Strengthen noclulation, especially rhizobium dross,

The blade of bigger,

The size of bigger,

Plant weight increase,

Plant fresh weight increases (FW),

Plant weights increase (DW),

Plant height increase,

Increased tiller number,

Increased young sprout growth,

Increased root growth (widely with system), the increased yield in lean soil or unfavourable weather,

The photosynthetic activity of enhancing,

Increased pigment content (such as chlorophyll content),

More prematurity,

Bear fruit earlier,

Earlier with improved budding,

Grain Ripening earlier,

More preferable size distribution,

The grain hardness of higher,

Improved self-defence mechanism,

Improved resistance and plant are to biological and abiotic stress resistance, such as fungi, bacterium, virus, insect, heat Stress, cold stress, drought stress, ultraviolet stress and/or salt stress,

Less unproductive tiller,

Less basal leaf is dead,

Required input is less (such as fertilizer or water),

Greener leaf,

The full maturity within the growth period of shortening,

Required fertilizer is less,

Required seed is less,

Easily harvest,

Faster, more consistent maturation,

Longer storage phase,

Longer panicle,

Aging postpones,

Stronger and/or more voluminous tiller,

Component has more preferable extractibility,

The seed quality (sow in subsequent season and produced for seed) of raising,

The ethene of reduction produces and/or suppresses reaction of the plant to it.

An embodiment according to the present invention, compared with each check plant being not handled by, according to improved characteristic Type plant growing way has been respectively increased at least 5%, 10%, 25%, 50%, or even more.

According to the plant of processing, different quality parameter is more preferably increased compared to other.According to the crop of processing, carry below To some mass parameters.

An embodiment according to the present invention, the preferred mass parameter of potato are：

Increased protein content (such as soluble protein),

Increased content of starch,

The fresh weight (FW) and/or dry weight (DW) of increased biomass such as higher,

Increased Zn content,

More preferable size distribution.

An embodiment according to the present invention, the preferred mass parameter of rice are：

Increased carotenoid content (such as vitamin A),

Increased Zn content,

Increased iron content,

Increased protein content (such as soluble protein).

An embodiment according to the present invention, the preferred mass parameter of corn are：

Increased carotenoid content (such as vitamin A),

Increased Zn content,

Increased iron content,

Increased protein content (such as soluble protein),

Increased oil content,

Increased content of starch.

An embodiment according to the present invention, the preferred mass parameter of apple are：

Increased calcium content,

The acidity of higher.

An embodiment according to the present invention, the preferred mass parameter of citrus are：

The increase of Vitamin C content.

The preferred mass parameter of an embodiment according to the present invention, tomato and cucumber is：

Increased calcium content.

An embodiment according to the present invention, the preferred mass parameter of grape are：

The anthocyanidin content of higher,

The acidity of higher.

The active ingredient containing micronutrient can be used as or with formulation customary in the art workable for the present invention Form uses, such as solution, emulsion, suspension, powder, paste.Then applied by this area conventional method.Therefore, For example, the available preparation for including the active ingredient containing micronutrient, as properly with other agricultural chemical activity compounds and Usual additive mixing is dressed seed.Also a kind of application type include by material workable for the present invention with itself or with Other agricultural chemical activity compounds and common dosage forms auxiliary agent are formulated as dosage form and are applied together, and have these preparations Solid plant inorganic agent is prepared, such as particle or bait.

When active ingredient containing micronutrient workable using the present invention, different according to application type, it is applied Dosage can change within the specific limits.When handling seed, the amount of application of the active ingredient containing micronutrient is general For every kilogram of 10~1000mg of seed, preferably every kilogram of 10~300mg of seed.When using solid dosage forms, contain micronutrient The amount of application of the active ingredient of element is generally every kilogram of 20~800mg of preparation, preferably every kilogram of 30~700mg of preparation.

The active ingredient containing micronutrient that the present invention uses generally with including it is at least one it is above-mentioned refer to contain The composition forms of the active ingredient of micronutrient are applied.Preferable Fungicidal composition includes that agricultural is acceptable to be added Add agent, solvent, carrier, surfactant or replenishers.

According to the present invention, carrier should be understood it is a kind of naturally either synthesis organic or inorganic material, its with Active ingredient is mixed or combined to reach more preferable applicability, is particularly applied to plant or plant part or seed.Carrier It can be solid or liquid, be generally inertia and be suitable for the material of agricultural.

Suitable solid or liquid-carrier have：Such as ammonium salt grinds mineral matter such as kaolin, clay, talcum with natural Powder, chalk, quartz, attapulgite, montmorillonite or diatomite, and the synthetic mineral matter ground such as fine grinding silica flour, aluminium oxide With natural or synthetic silicate, resin, wax, solid fertilizer, water, alcohols, especially butanol, organic solvent, mineral oil With vegetable oil and its derivative.Also these carriers can be used with.Solid carrier is suitable for having for granule：Such as crush and be classified Natural mineral matter such as calcite, marble, float stone, sepiolite, dolomite, while also include some synthesis it is organic and inorganic Food granule and organic material particles such as sawdust, cocoanut shell, corncob and tabacco straw.

Suitable liquid gas replenishers or carrier are all liquid, are at room temperature and atmospheric pressure gaseous state, such as aerosol Propellant such as butane, propane, nitrogen and carbon dioxide etc..

The polymer of tackifier such as carboxymethyl cellulose and natural and synthesis powder, particle and latex type, such as me Uncle's natural gum, polyvinyl alcohol, polyvinyl acetate, or other natural phospholipids, as cephalin, lecithin and synthetic phospholipid also can be Used in formula.Other possible additives are mineral and vegetable oil and wax, are optionally modified.

If using water as replenishers, secondary solvent for example can also be used as by the use of organic solvent.Suitable organic solvent Mainly have：Aromatic compound, such as dimethylbenzene, toluene or alkylnaphthalene, chloride aromatic compound；Or the chemical combination of chloride aliphatic hydrocarbon Thing, such as chlorobenzene, vinyl chloride or dichloromethane；Aliphatic hydrocarbon, such as hexamethylene or paraffin；Such as mineral oil fractions, mineral matter and plant Oil；Alcohols, such as butanol or ethylene glycol, and its ether and esters；Ketone, such as acetone, methyl ethyl ketone, hexone or hexamethylene Ketone；Intensive polar solvent, such as dimethylformamide and dimethyl sulfoxide (DMSO) and water.

Constituent according to the present invention may also include other components, such as surfactant.Suitable surfactant There are emulsifying agent, dispersant or the wetting agent with ion or non-ionic nature, or the mixture of these surfactants.These Example have polyacrylate, lignosulphonates, phenolsulfonate, naphthalene sulfonate, ethylene oxide and fatty alcohol or and aliphatic acid Or with the condensation polymer of fatty amine, the phenol (optimizing alkyl phenol or aryl phenol) of substitution, phosphate ester salt, taurine derivatives (preferably Taurine Arrcostab), phosphate, the polyol fat ester of polyethoxylated alcohols or phenols, and contain sulfate, sulfonate With the derivative of phosphate compounds.When in applied to water, if a kind of active ingredient therein and/or a kind of inert carrier It is insoluble in water, then surfactant is then required.The ratio of surfactant is calculated as by present composition weight 5%~40%.

It can use colouring agent such as inorganic dyestuff, for example, it is iron oxide, titanium oxide, Prussian blue, and organic dyestuff, it is such as alizarin Uniformly dyeing material, azo dyes and metallized phthalocyanine dye, and micronutrient, such as molysite, manganese salt, boron salt, mantoquita, cobalt salt, molybdenum salt And zinc salt.

If suitable, there may also be other annexing ingredients, such as protective colloid, binding agent, adhesive, thickening Agent, thixotroping material, penetrating fluid, stabilizer, chelating agent, complexing agent.In general, usually can be by reactive compound with being usually used in The solid or liquid additive of preparation are used in combination.

In general, composition according to the present invention includes being 0.05%~99%, 0.01%~98% by weight, preferably 0.1%~95%, particularly preferred 0.5%~90% active ingredient combinations, are most preferably 10%~70%.

The combination of the reactive compound of the present invention or composition can be used or according to their own physics with itself And/or chemical characteristic, the application form in the form of its preparation or thus prepared, as aerosol, capsule suspension, harl agent, Thermal fog, encapsulation particle, fine grained, the suspending agent for handling seed, instant solution, pulvis, emulsifiable concentrate, oil-in-water breast Agent, water-in-oil emulsion, big granula, fine granule, oil-dispersing property pulvis, oil-suspending agent, oil-soluble liquor, foaming agent, paste, pesticide The seed of coating, suspension concentrates, dense suspension emulsion, solvable concentrate, suspension, wettable powder, soluble powder, powder and Granula, water-soluble granular formulation or tablet, handle the water solube powder, wettable powder, the natural production rich in active ingredient of seed Thing and synthetic, also include macromolecule and Coating Materials microcapsules applied to seed, and the agent of ULV harls and thermal fog.

The preparation referred to can be prepared with a kind of known method, for example, by mixture active ingredient or by activity into Divide and combined with least one additive.Suitable additive is all common dosage forms auxiliary agents, for example, organic solvent, replenishers, Solvent or diluent, solid carrier and filler, surfactant (such as adjuvant, emulsifying agent, dispersant, protective colloid, wetting Agent and tackifier), dispersant and/or binding agent or fixative, preservative, dyestuff and pigment, defoamer, inorganic and organic thickening Agent, waterproofing agent, also drier and UV stabilizer, gibberellin if applicable, also have water and the auxiliary agent further handled. Prepare the processing procedure of next step in the case of each according to formulation, if desired for wet-milling, dry grinding or granulation.

The present composition not only includes can use appropriate equipment to be applied to the instant composition on plant or seed, also wraps Include the commercial concentrate formulation that must be diluted with water before use.

The present invention active compound can (commercial) preparation and the conduct prepared by these preparations and other The application form of the mixture of (known) active ingredient, such as insecticide, attractant, disinfectant, fungicide, acaricide, nematicidal Agent, fungicide, growth regulator, herbicide, fertilizer, safener and semiochemical.

According to the present invention directly plant or plant parts are handled with active ingredient or by acting on its surrounding environment, dwelling Breath ground or storage space, its using conventional treatment method, such as by impregnating, spraying, spraying, irrigating, evaporating, dusting, It is atomized, sows, blistering, smearing, paving, pouring (spray), trickle irrigation is handled；In the case of brood body, especially in sub-cases Under, and be the powder as processing dry seeds, handle the solution of seed, the water-soluble powder of pulp processing, by carrying out one Layer or multilayer cladding or coating etc. are handled.Additionally the reactive compound may be applied by ultralow volumetric method, or will Active agent preparations or reactive compound inject soil in itself.

Present invention additionally comprises a kind of method for handling seed.The invention further relates to according to one kind side referred in aforementioned paragraphs The seed of method processing.

The reactive compound or composition of the present invention is especially suitable for handling seed.One as caused by harmful organism Most of Crop damage, is during the storage of seeds or after planting and caused by the infection during germination or after germination.This A stage is even more important, because the plant roots and the particularly sensitive or even small infringement of bud in growth may result in the dead of plant Die.Therefore, the use of appropriate composition is very useful when protecting seed and sprouting plant.

Control plant pathogenic fungi to be early well known by handling vegetable seeds, and be a class persistently improved Topic.However, processing seed is involved in the problems, such as that a series of cannot find is satisfied with solution.Accordingly, it is desirable to research and develop protection kind Son and the method for sprouting plant, it can be with without using plant protection product with extra after plant germination at seeding time, or at least Greatly reduce extra use.It is desirable that can be by optimizing the amount of application of reactive compound, so that being seed and sprouting Plant provides maximum protection and is damaged against plant pathogenic fungi, and applied active ingredient does not damage plant itself.Particularly, Processing seed method should also be as consider genetically modified plants in fungicidal properties, with realize using minimum crop protect Shield agent reaches to seed and sprouts the optimal protection of plant.

Therefore, the present invention is also specifically related to a kind of method for handling seed and plant germination, to improve plant quality.

It is also regarded as it is advantageous that the method for the present invention can also be specifically used on transgenic seed, by this The plant that seed grows up to can express a kind of insect resistance protein.By using the active compound or compositions-treated kind of the present invention Son, or even by expression such as insecticidal proteins, can control some pests.It was unexpectedly determined that it is observed that further collaboration Effect, it more improves the quality through handling plant.

The composition of the present invention is suitably applied agricultural, greenhouse, forest, gardening or the seed of viticultural all plants Processing.Particularly, the seed (such as wheat, barley, rye, triticale, millet, oat) of cereal form, corn, cotton, soybean, Rice, potato, sunflower, beans, coffee, beet (such as sugar beet and fodder beet), peanut, rape, poppy flower, olive, Coconut, cocoa, sugarcane, tobacco, vegetables (such as tomato, cucumber, onion, romaine lettuce), meadow and ornamental plant (seeing below).To cereal The processing of class (such as wheat, barley, rye, triticale and oat), corn and rice paddy seed is even more important.

Further illustrate further below, it is especially heavy with the active compound or compositions-treated transgenic seed of the present invention Will.Vegetable seeds described herein includes at least one foreign gene that can express more peptide or proteins with insecticidal properties. The foreign gene in transgenic seed can be derived from microbial species such as bacillus, rhizobium, pseudomonad, sand Thunder Salmonella, Trichoderma, corynebacteria, sacculus mould or glue branch mould.Preferable foreign gene is derived from bacillus, its base Because product has the activity for killing European corn borer and/or western corn snout moth's larva.Particularly preferred foreign gene is derived from Su Yunjin Bacillus (Bacillus thuringiensis).

In the context of the present invention, active compound of the invention or composition are applied individually or with appropriate preparation For seed.The state of preferred process seed is sufficiently stable so as to which the processing will not cause any infringement for its.It is, in general, that can Seed is handled with any time point between plant seeding and harvest.Conventionally used seed from the cob of plant, Separate or be stripped out in housing, stalk, coating, hair or flesh.Therefore, such as harvest, cleaning can be used simultaneously The dry seed that 15% weight is less than to moisture.Alternatively, can also use it is dry after for example with water-treated and do again Dry seed.

When handling seed, the dosage of the present composition that generally must be noted that to consider to be applied to seed and/or selected The dosage for the additional additive selected, would not be adversely affected so as to the seed of sprouting, or final plant will not be damaged Evil.Must especially it keep firmly in mind, active ingredient might have biological detoxifying function at certain application rates.

The composition of the present invention can be applied directly, that is to say, that, it is not necessary to, also need not be further including further component Dilution.In general, it is preferred to the composition is applied to seed with suitable dosage form.Handle seed appropriate formulation and Method known to those skilled in the art and is described in, such as the following files：US 4,272,417、US 4,245,432、 US 4,808,430、US 5,876,739、US 2003/0176428 A1、WO 2002/080675、WO 2002/028186。

The active compound of the present invention is convertible into usual Seed dressing formulations, such as solution, emulsion, supensoid agent, powder Agent, foaming agent, slurries agent or other be used for seed Coating Materials, also have ULV preparations.

These preparations are prepared with known method, and reactive compound or active compound are mixed with typical additives Close, it is such as common replenishers and solvent or diluent, colouring agent, wetting agent, dispersant, emulsifying agent, defoamer, preservative, secondary Raw thickener, adhesive, gibberellin and water.

The Suitable colouring agents that may be present in the Seed dressing formulations that can be used in the present invention include being useful for the normal of this purposes Use colouring agent.The pigment being slightly soluble in and the dyestuff for being dissolved in water can be used.The colouring agent example that can be mentioned includes known title Rhodamine B, C.I. pigment red 112s and C.I. solvent reds 1.

May be present in can be used in the present invention Seed dressing formulations in Suitable humectants include it is all be used for promote wetting and For the common material of active agrochemical substance preparation.

It is active that the suitable dispersant and/or emulsifying agent that may be present in the Seed dressing formulations that can be used in the present invention include institute Common nonionic, anion and cation dispersing agent in agrochemical substances preparation.It is preferred that nonionic or anion can be used Dispersant or nonionic or anionic dispersant agent composition.The non-ionic dispersing agent being particularly suitable has：Ethylene oxide-epoxy third Alkane block copolymer, alkyl phenol polyethylene glycol ethers and tristyryl phenol polyglycol ether, and their phosphate or sulphur Acid salt derivant.Particularly suitable anionic dispersing agents have：Lignosulfonate, polyacrylate and aryl sulphonate-formaldehyde Condensation product.

The suitable defoamer that may be present in the Seed dressing formulations for the present invention is activated including all active agrochemicals The common compound for suppressing foam in compound preparation.Preferably using silicone antifoams agent, magnesium stearate, silicone emulsion, higher alcohols, Aliphatic acid and their salt and organic fluoride and its mixture.

The suitable preservatives that may be present in the Seed dressing formulations for the present invention include all tools in agrochemical composition There is the compound of this purposes.By way of example, Dichlorophenol and Bian alcohol hemiformal be there can be mentioned.

The suitable secondary thickener that may be present in the Seed dressing formulations for the present invention includes institute in agrochemical composition There is the compound with this purposes.Preferably cellulose derivative, acrylic acid derivative, polysaccharide such as xanthans or aluminium-magnesium silicate, Modified clay, phyllosilicate such as attapulgite and bentonite and superfine silicic acid.

The proper adhesive that may be present in the Seed dressing formulations for the present invention includes all common bondings for dressing seed Agent.It is preferred that it there can be mentioned：Polyvinylpyrrolidone, polyvinyl acetate, polyvinyl alcohol and thylose.

May be present in for the present invention Seed dressing formulations in suitable gibberellin be preferably gibberellin A1, A3 (=it is red mould Acid), A4 and A7, particularly preferably using gibberellic acid.Gibberellin is known (to contrast R.Wegler " Chemie der Pflanzenschutz and-”[Chemistry of Crop Protection Agents and Pesticides],Vol.2,Springer Verlag,1970,pp.401-412)。

Seed dressing formulations for use in the present invention can be used directly or be diluted with water in advance the various types of seeds of post processing.Can It can be used for the seed dressing of genetically modified plants for the Seed dressing formulations of the present invention or its dilution preparation.In this context, produced with expression The interaction of thing can produce cooperative effect.

Add the preparation of water preparation using Seed dressing formulations for use in the present invention or by it, handle the convenient mixing device of seed Including all mixing apparatus that usually can be used to dress seed.The detailed process used during seed dressing includes, and seed is added in mixer, The specific required amount of Seed dressing formulations of addition, are directly added into itself or are diluted with water in advance, mixed until described Preparation is uniformly distributed on seed.Optionally, operation is then dried.

According to the present invention, all plants and plant parts can be carried out handling.Plant refers to all plants and plant Whether population (is subject to Plant variety right or plant to educate such as wild plant, cultivar and plant variety can use and worthless The protection of kind person's right).Cultivar and plant variety can be the plants obtained by traditional mating system, and the method can Aided in or supplemented by one or more biological technique methods, such as by using dihaploid, protoplast fusion, random With directed mutagenesis, molecule or genetic marker or pass through bioengineering and gene engineering method.Plant parts refer to all ground Upper and under ground portion and plant organ, such as bud, leaf, Hua Hegen, are thus listed for example, leaf, needle, stem, branch, flower, fructification, fruit Real and seed and root, stem tuber, bulb and root-like stock.Crop and nourish and generate and case of propagation material, for example, cutting, bulb, Root-like stock, stem tuber, stolon and seed also belong to plant parts.

The reactive compound of the present invention is combined with good Plant Tolerance and to the favourable toxicity of warm-blooded animal, can be by environment very Good receiving, this is adapted to protect plant and plant organ, to improve harvest, improves the quality of harvest material.They may be preferably As crop protection agents.They for normal sensitive and resistant species and have work for all or some growth phases Property.

Can by the present invention method handle plant in, it may be mentioned that have main field crops as corn and soybean, cotton Flower, Btassica rape such as colea (Brassica napus) (such as La Nuola (canola) rape), turnip (Brassica Rapa), mustard type rape (B.juncea) (such as mustard) and brassicacarinata (Brassica carinata), rice, small Wheat, beet, sugarcane, oat, rye, barley, millet, triticale, flax, grape and various fruit and various plants The vegetables such as rose family of group is (for example, pip fruit also has drupe as apricot, cherry, almond and peach, berry as apple and pears Class fruit is as strawberry), Grossulariaceae, Juglandaceae, Betulaceae, Anacardiaceae, Fagaceae, Moraceae, Oleaceae, Actinidiaceae, camphor tree Section, Musaceae (such as Banana tree and plant), Rubiaceae (such as coffee), Theaceae, Sterculiaceae, Rutaceae (such as lemon, orange and Portugal Grape shaddock), Solanaceae (such as tomato, potato, pepper, eggplant), Liliaceae, composite family (such as romaine lettuce, globe artichoke and witloof-include cichory root, Hare's-lettuce or bitter dish), Umbelliferae (such as carrot, caraway, celery and celeriac), Curcurbitaceae (such as cucumber-include salting cucumber, south Melon, watermelon, cucurbit and muskmelon), green onion section (such as onion and leek), Cruciferae (such as cabbage, violet cabbage, broccoli, cauliflower, Small cabbage, pakchoi, wild cabbage, radish, horseradish, Chinese celery, Chinese cabbage), pulse family (such as peanut, pea and Kidney bean, bean or pea-akala bean or pea With broad bean, Chenopodiaceae (such as sugar lettuce, Radix Betaes, spinach, beet root), Malvaceae (such as gumbo), Asparagaceae (such as asparagus), gardening And forest crop, ornamental plant, and the transgenosis homologue of these crops.The particularly important is to rice, corn, potato, The processing of cereal and grape.

As already mentioned above, all plants and its position can be handled according to the present invention.In a preferable implementation In scheme, wild plant species and cultivated plant kind are handled, or those pass through traditional biological breeding method such as hybridization or former Plant that raw plast fusion obtains or part thereof.In a further preferred embodiment, genetically modified plants have been handled and have been led to The cultivated plant of genetic engineering acquisition is crossed, if the plant of appropriate combination conventional method (genetically modified organism) acquisition or its portion Point.Term " part ", " part of plant ", " plant part " have been carried out explaining above.In particular it is preferred that according to this The plant of the cultivated plant of processing is invented, it all can buy or use under any circumstance.Plant cultivars should Being understood to mean plant has new features (character), it is obtained by traditional breeding, gene mutation or by DNA recombinant techniques .These can become cultivar, biology or genotype.

The processing method of the present invention can be used for handling genetically modified organism (GMOs), such as plant or seed.Genetic modification Plant (or genetically modified plants) is the plant that foreign gene stable integration enters genome.Term " foreign gene " is substantially a kind of The gene for providing and assembling outside plant, when it is introduced into nucleus, chloroplaset or mitochondrial genomes, by expressing mesh Albumen or polypeptide, or make other be present in the gene deregulation of plant or silence (application as antisense technology, co-suppression technology or RNA interference-RNAi technology), thus assign be converted plant it is new or the economical character of improvement or other characteristics.Foreign gene quilt It is positioned in genome and is called transgenosis.Transgenosis is defined because of its specific location in Plant Genome, is referred to as turning Change or transgenic event.

According to floristics and plant variety, the location of they and growth conditions (soil, weather, growth period, battalion Support), treatment in accordance with the present invention method may cause the effect of superadditivity (" collaboration ").Thus, for example, in fact it could happen that it is following super Cross the effect of actual desired：Reduce amount of application and/or widen reactive compound of the present invention workable for field of activity and/or increase With the activity of composition, can make plant growth obtain more preferably, tolerance of the enhancing to high temperature or low temperature, enhancing is to drought or flood or soil The tolerance of salt content, strengthen the performance bloomed, be more easy to harvest, hasting of maturity, the crop of higher, the fruit of bigger, higher Plant, greener leaf, more prematurity, harvest product higher quality and/or the nutritive value of higher, fruit higher contains Sugar amount, more preferable bin stability and/or the processing possibility for harvesting product.

At certain application rates, active compound of the invention can also fortification of plants.Therefore they also be adapted for for The system of defense of plant is transferred to resist harmful microorganism.This is probably that activity of the invention is improved as antimycotic if appropriate A reason.In the context of the present invention, the material of fortification of plants (induction of resistance) be understood to mean that these materials or Ability of the material combination with activated plant defense system, i.e., ought then be inoculated with harmful microorganism, processed plant to The resistance in substantial extent is shown when resisting these microorganisms.Under existing conditions, harmful microorganism can be understood as planting Thing pathogenic epiphyte, bacterium and virus.Therefore, material of the invention can be used for, and after treatment in one period, protection plant is exempted from Injured by pathogen mentioned above.After plant is handled with the reactive compound, to the protecting effect one of plant generation As extend to 1~10 day, preferably 1~7 day.

, according to the invention it is preferred to the plant handled and Plant cultivars, which include having, can assign these plants spy All plants of the inhereditary material of not favourable, useful character.(no matter being obtained by breeding and/or animal nutrition).

, according to the invention it is preferred to the plant handled and Plant cultivars are coerced for that can resist one or more biologies Urgent plant, that is to say, that the plant is to resist animal and microbial pests such as nematode, insect, mite class, plant-pathogenic true When bacterium, bacterium, virus and/or viroid, stronger resistance is shown.

The example of plant with nematode resistance is described in following U.S. Patent application：No’s11/765,491,11/ 765,494,10/926,819,10/782,020,12/032,479,10/783,417,10/782,096,11/657,964,12/ 192,904,11/396,808,12/166,253,12/166,239,12/166,124,12/166,209,11/762,886,12/ 364,335,11/763,947,12/252,453,12/209,354,12/491,396 and 12/497,221.

The plant that can be handled according to the present invention is to have given expression to the hybrid plant of hybridization or hybrid vigour feature, the spy Point usually comes at high yield, vigor, health and the ability for resisting biology and abiotic stress.This plant usually passes through a kind of male Sterile self-mating system parent (female parent) and another male sterility self-mating system parent (male parent) hybridization obtain.Usually from male sterile line Harvest hybrid seed is simultaneously sold to farmer.Male sterile plants (such as corn) are obtained sometimes through emasculation, that is, mechanically Male reproductive organ (or male flower) is removed, but more typically male sterility is the gene-determined of Plant Genome.This In the case of, especially when seed is intended to from the product of hybrid plant harvest, determine that hybrid plant male fertility is completely extensive It is highly useful again.This point can have suitable restoring gene by ensuring male parent, can recover male containing determining The male fertility of the hybrid plant of sterile gene is realized.Male sterile genetic determinant may also be located at cytoplasm In.The example of cytoplasmic male sterility (CMS), such as Brassica plants are described.However, male sterility genetic determinant It may be alternatively located in Matrix attachment region.Male sterile plants can also be obtained by such as genetic engineering of Plant Biotechnology method.Obtain Male sterile plants particularly useful method is obtained to be described in WO 89/10396, for example, ribalgilase, such as bacillus RNase selective expression in the tapetal cell of stamen.Then suppressed by expressing ribalgilase in tapetal cell Son recovers fertility such as barstar.

According to the present invention, the plant that can be handled or Plant cultivars (pass through Plant Biotechnology such as genetic engineering Obtain) it is herbicide-resistant plant, that is to say, that and plant has tolerance to one or more herbicides of administration.The plant can lead to Genetic transformation is crossed, or is obtained by screening the plant containing the mutation for assigning the herbicide tolerant.

The plant of antiweed has such as glyphosate-tolerant plant, i.e., has tolerance to herbicide glyphosate or its salt Plant.Plant can obtain glyphosate tolerant by a variety of methods.Such as the tolerance plant of glyphosate can be by using coding Enzyme 5- enol pyruvylshikimate -3- phosphate synthases (EPSPS) gene carry out conversion plant obtain.EPSPS genes Example has AroA genes (CT7 mutant) (Comai et of salmonella typhimurium (Salmonella typhimurium) Al., 1983, Science 221,370-371), the CP4 genes of Agrobacterium bacterium (Barry et al., 1992, Curr.Topics Plant Physiol.7,139-145), coding petunia EPSPS (Shah et al., 1986, Science 233,478-481), tomato EPSPS (Gasser et al., 1988, J.Biol.Chem.263,4280-4289) orBelong to The gene of EPSPS (WO 01/66704).It is alternatively the EPSPS of mutation.Glyphosate-tolerant plant can also be encoded by expression The gene of glyphosate oxidoreductase obtains.Glyphosate-tolerant plant also can encode glyphosate acetyl based transferase by expression Gene obtain.Glyphosate-tolerant plant can also be obtained by screening the plant being mutated containing naturally occurring said gene .The plant that expression assigns the EPSPS genes of plant glyphosate-tolerant has been described.The gene of other conferring glyphosate tolerances Plant such as decarboxylase gene is also described.

Other herbicide tolerant plants are herbicide such as double propylamine phosphines, grass for example, to suppressing glutamine synthelase Fourth phosphine or glufosinate-ammonium have the plant of tolerance.The plant can release the enzyme of herbicide toxicity or the mutation of anti-suppression by expressing Glutamine synthelase obtains.The detoxication enzyme for having such effect is coding glufosinate transacetylase (as come from streptomyces Bar or pat albumen) enzyme.The plant of expression external source glufosinate transacetylase has been described.

The plant of other herbicide-resistants is the resistance to plant for suppressing hydroxyphenyl pyravate dioxygenase (HPPD) herbicide.HPPD It is the enzyme for being catalyzed the reaction that hydroxyphenyl pyravate is converted into alcapton.Plant is natural to the tolerance available code of HPPD inhibitor The gene of the genes of existing resistance HPPD enzymes or encoding mutant or chimeric HPPD enzymes is converted, such as WO 96/38567, Described in WO 99/24585, WO 99/24586, WO 2009/144079, WO 2002/046387 or US 6,768,044. The resistance to HPPD inhibitor can be also obtained with the gene-transformed plant for encoding some enzymes, although these enzymes to suppress in HPPD Agent suppresses still form alkapton in the case of natural HPPD enzymatic activitys.Such plant and gene are in WO 99/34008 and WO It is described in 02/36787.Plant in addition to the gene of conversion coding HPPD resistance enzymes, also may be used the tolerance of HPPD inhibitor Improved with the gene-transformed plant by using enzyme of the coding with prephenate dehydrogenase (PDH) activity, such as WO 2004/ Described in 024928., can be by its gene in addition, make plant obtain to the stronger tolerance of HPPD- inhibitor herbicides Addition coding is metabolizable in group or the gene of the enzyme of degraded HPPD- inhibitor is realized, such as WO 2007/103567 and WO CYP450 enzymes described in 2008/150473.

Also other herbicide resistant plants are the plants of resistance to acetolactate synthestase (ALS) inhibitor.Known ALS suppresses Agent includes, for example, sulfonylurea, imidazolone type, triazolo pyrimidine class, pyrimidine radicals oxygen (thio) benzoic ether and/or sulphonyl Amino carbonyl triazole quinoline herbicides.The different mutation (known Acetohydroxyacid synthase, AHAS) of known ALS enzymes impart To different herbicides and the other tolerance of classes of herbicides, for example, such as in Tranel and Wright (2002, Weed Science 50:Described in 700-712).Describe the production to sulfonylureas tolerant plants and imidazolinone-tolerant plant.Also describe Other imidazolinone-tolerant plants.Also illustrate other sulfonylureas and imidazolinone-tolerant plant.

Other can obtain the plant of imidazolone and/or sulfonylurea with tolerance by mutagenesis, containing except Screened in the cell culture fluid of careless agent or mutation breeding, for example, soybean is described in United States Patent (USP) No.5,084,082, water Rice is described in WO 97/41218, and beet is described in United States Patent (USP) No.5, and 773,702 and WO 99/057965, romaine lettuce is described in U.S. State patent No.5,198,599, sunflower is described in WO 01/065922.

According to the present invention, accessible plant or plant variety (being obtained by biological technique method such as genetic engineering) are anti- Worm genetically modified plants, that is plant can resist the attack of some specific insects.The plant can be by genetic transformation, or passes through sieve Select the plant containing the mutation for assigning this insect-resistant and obtain.

" insect-resistant transgenic plants " used herein include any plant containing at least one transgenosis, the transgenosis Include the coded sequence for encoding following albumen：

1) the insecticidal crystal albumen from bacillus thuringiensis (Bacillus thuringiensis) or its desinsection portion Point, such as Crickmore et al. (1998, Microbiology and Molecular Biology Reviews, 62:807- 813) the insecticidal crystal albumen listed by, Crickmore et al. (2005) are to B. thuringiensis Toxin or insecticidal part Name is updated, for example, crystalline protein albuminoid Cry1Ab, Cry1Ac, Cry1B, Cry1C, Cry1D, Cry1F, Cry2Ab, Cry3Aa, or Cry3Bb, or its insecticidal part (such as EP 1999141 and WO 2007/107302), or have what synthetic gene encoded The albumen, such as U.S. Patent application No 12/249, described in 016；

2) crystalline protein from bacillus thuringiensis or its part with insecticidal action, it is from Su Yun gold buds There is insecticidal activity, such as by Cry34 and Cry35 crystalline protein groups in the presence of the second of spore bacillus other crystalline proteins or part thereof Into binary toxin (Moellenbeck et al.2001, Nat.Biotechnol.19:668-72；Schnepf et Al.2006, Applied Environm.Microbiol.71,1765-1774) or Cry1A or Cry1F albumen and Cry2Aa or Binary toxin (the US Patent Appl.No.12/214,022and EP of Cry2Ab or Cry2Ae albumen composition 08010791.5)；

3) the hybrid insecticidal proteins of the different insecticidal crystal albumen from bacillus thuringiensis are included, such as above-mentioned 1) miscellaneous Kind albumen or above-mentioned hybrid proteins 2), such as the Cry1A.105 albumen (WO produced by corn transformation event MON 89034 2007/027777)；

4) above-mentioned 1) to 3) any albumen, some of which amino acid, especially 1-10 amino acids are by other amino Acid is substituted to obtain to the insecticidal activity of targeted insect higher and/or widen coverage to targeted insect, and/or due to Change is introduced in coding DNA in clone or conversion process, such as the Cry3Bb1 of corn transformation event MON863 or MON88017 Albumen, or the Cry3A albumen of corn transformation event mir 604；

5) the desinsection secretory protein from bacillus thuringiensis or Bacillus cercus (Bacillus cereus), or Its insecticidal part, such as trophophase desinsection (VIP) albumen that given data storehouse is listed, the albumen from VIP3Aa protides；

6) secretory protein from bacillus thuringiensis or Bacillus cercus, its from bacillus thuringiensis or There is pesticidal, the binary toxin being such as made of VIP1A and VIP2A albumen in the presence of second secretory protein of Bacillus cercus (WO 94/21795)；

7) hybrid that the part comprising the different secretory proteins from bacillus thuringiensis or Bacillus cercus forms Insecticidal proteins, such as above-mentioned hybrid proteins 1) or above-mentioned hybrid proteins 2)；

8) above-mentioned 5) to any albumen in 7), the amino acid of some of which amino acid, especially 1-10 is by other amino Acid, which substitutes, to be obtained to the insecticidal activity of targeted insect higher, and/or widens the coverage to targeted insect, and/or due to Clone or conversion process in, by change be incorporated into the DNA of coding (still codified insecticidal proteins), such as corn transformation event The VIP3Aa albumen of COT102；Or

9) secretory protein from bacillus thuringiensis or Bacillus cercus, it is from from Su Yun gold gemma bars There is pesticidal, to be formed such as VIP3 and Cry1A or Cry1F binary toxin (US Patent in the presence of the crystalline protein of bacterium Appl.No.61/126083 and 61/195019), or be made of VIP3 albumen and Cry2Aa or Cry2Ab or Cry2Ae albumen Binary toxin (US Patent Appl.No.12/214,022 and EP 08010791.5)；

10) above-mentioned 9) described albumen, the amino acid of some of which amino acid, especially 1-10 is by other amino acid replacements Obtain to the insecticidal activity of targeted insect higher, and/or widen the coverage to targeted insect, and/or due in clone or In conversion process, it is incorporated into changing in the DNA of coding (still codified insecticidal proteins).

Certainly, insect-resistant transgenic plants used herein, further include the albumen containing coding any one of above-mentioned 1 to 10 Any plant of the assortment of genes of matter.In one embodiment, insect-resistant transgenic plants are included in a variety of above-mentioned 1 to 10 classes and appointed A kind of transgenosis of albumen, when acting on different target insect with different albumen, expands the coverage of targeted insect, or Same target insect is acted on by using different insecticidal proteins to postpone resistance development of the insect to plant, but it has not The same mode of action, for example it is incorporated into the different receptor binding site of insect.

" insect-resistant transgenic plants " used herein, further include any plant containing at least one transgenosis, described to turn base Because suppressing the growth of the pest when being absorbed by plant insect containing sequence, the RNA caused by expression double-stranded RNA.

The also accessible plant of the present invention or Plant cultivars (being obtained by Plant Biotechnology such as genetic engineering) are The plant of resisting abiotic stress.The plant can assign the anti-stress ability by genetic transformation, or by screening to contain The plant of mutation obtains.Particularly useful anti-stress plant includes:

1) containing turn that can reduce (ADP- ribose) polymerase (PARP) expression poly- in plant cell or plant and/or activity The plant of gene.

2) plant containing the transgenosis for strengthening resistance to stress ability, the transgenosis can be reduced to be compiled in plant or plant cell The expression of the gene of code PARG and/or activity.

3) plant of the transgenosis containing enhancing stress ability, the transgenes encoding nicotinamide adenine dinucleotide are mended Rescue the plant function enzyme of route of synthesis, including nicotinamidase, nicotinoyl acid ribose phosphate transferase, nicotinic acid single nucleotide adenyl Transferase, nicotinoyl adenine-dinucleotide synzyme or Nicotinic Acid Amide phosphorylation transferase.

The accessible plant of the present invention or Plant cultivars (being obtained by Plant Biotechnology method such as genetic engineering) Harvest product show to change quantity, quality and/or storage stability and/or the characteristic for changing harvest product special component, Such as：

1) genetically modified plants of synthesis modification starch, compared with the plant cell of wild type or plant, the reason of modified starch Change the content or straight chain/amylopectin ratio, the degree of branching, average chain length, side chain distribution, viscosity of property, especially amylose Performance, gel strength, amylum body size and/or morphology of starch grain have change.Therefore this is more suitable for special applications.

2) genetically modified plants, it synthesizes non-starch carbohydrate polymer, or with not carrying out the wild type of genetic modification Plant compares the non-starch carbohydrate polymer of synthesis modification.The example is the plant of production polyfructosan, especially synanthrin Type and fructan-type, produce the plant of α-Isosorbide-5-Nitrae-glucan, produce α -1, the plant of 6 α-Isosorbide-5-Nitrae glucan, produces alternan (alternan) plant.

3) genetically modified plants of hyaluronic acid are produced.

4) transgenosis or hybrid plant, such as have ' high-content soluble solids ', ' low acid ' (LP) and/or ' storage Phase is grown ' onion of characteristic.

The accessible plant of the present invention or Plant cultivars (being obtained by Plant Biotechnology method such as genetic engineering) To change the plant of cellulosic nature such as cotton.The plant can change by genetic transformation, or by screening containing this is assigned The plant of the mutation of the cellulosic nature of change obtains, including：

A) plant of the cellulose synthase gene changed containing form, such as cotton.

B) rsw2 the or rsw3 homologous nucleic acids plant such as cotton changed containing form, Sucrose Phosphate Synthase up-regulated expression Plant such as cotton.

C) plant of sucrose synthase up-regulated expression such as cotton.

D) for example, by lowering fiber selectivity β -1,3- dextranase, fibrocyte base portion plasmodesmus gate is changed The plant of time such as cotton.

E) for example, passing through N-acetylglucosamine transferase gene and the table of chitin synthetase gene including nodC Reach, the plant such as cotton has the cellulose for changing reactivity.

The accessible plant of the present invention or Plant cultivars (being obtained by Plant Biotechnology method such as genetic engineering) To change the plant of the ingredient properties of oil, such as rape or relevant Brassica plants.The plant can by genetic transformation, or Obtained by screening containing the plant that can assign the mutation for changing oil component characteristic, including：

A) the oily plant with high oleic acid content, such as rape are produced.

B) the oily plant with low linolenic, such as rape are produced.

C) the oily plant with low-level saturated fatty acid, such as rape are produced.

The accessible plant of institute of the invention or Plant cultivars (are obtained by Plant Biotechnology method such as genetic engineering ) it is such as potato of the plant with virus resistance, for example, the anti-Potyvirus Y (transformation events from Tecnoplant SY230 and SY233, Argentina)；It is to be disease-resistant such as the plant of anti-late blight of potato (such as RB genes)；It shows cold-induced Sweetened reduction (carrying Nt-Inhh, IIR-INV genes) has Dwarfing phenotypes (A-20 oxidase genes).

The accessible plant of the present invention or Plant cultivars (being obtained by Plant Biotechnology method such as genetic engineering) To change the plant of seed breakage properties, such as rape or relevant Brassica plants.The plant can by genetic transformation, or Obtained by screening containing the plant that can assign the mutation for changing seed breakage properties, including with such as kind of delay or reduction The broken plant such as rape of son.

The accessible genetically modified plants with special applications value of the present invention are to contain transformation event or and transformation event The plant of combination, these are to apply in the U.S. to the animal of United States Department of Agriculture (USDA) with plant health Quarantine Bureau (APHIS) The theme of non-control state, and though this application whether approved or still in examination.Whenever this information can be from APHIS (4700 River Road Riverdale, MD 20737, USA) is obtained.Day is declared in the non-control state application Phase, APHIS include following information in the application examined or APHIS has been approved by：

- the petition：The numbering of the petition.The technology description of transformation event can be in the single request text obtained from APHIS Found in part, such as the website in APHIS, by reference to the numbering of this petition.These descriptions are hereby incorporated by reference.

The extension of-the petition：With reference to the earlier application for requiring to delay.

- mechanism：Submit the title of petition unit.

- control project：Related plant species.

- transgenic phenotypes：The plant characteristic that transformation event assigns.

- transformation event or strain：It is required that the transformation event title (sometimes referred to as strain) of non-control state.

- APHIS documents：Can be required by APHIS and by the APHIS issues and relevant various files of the petition.

The accessible genetically modified plants with special applications value of the present invention are comprising more than one coding one or more The plant of the gene of a toxin, the plant such as sold below with trade name：YIELD(such as corn, cotton, soybean),(such as corn),(such as corn),(such as corn),(such as jade Rice),(cotton),(cotton), Nucotn 33B (cotton),(such as corn),With(potato).It can be mentioned that the examples of herbicide tolerant plants be maize varieties, cotton Flower mutation and soya bean varieties, they are sold with following trade names：Roundup(glyphosate tolerant, such as corn, cotton, big Beans), Liberty(resistance to glufosinate, such as rape),(resistance to imidazolone type) and(resistance to sulfonylurea, it is such as beautiful Rice).The herbicide-resistant plant (the herbicide-resistant plant bred in a usual manner) that can be mentioned that include it is various with The plant of (such as corn) title sale.

Other plants with special applications value for including independent transformation event or combining transformation event, for example, coming Listed in the database from the regulator in country variant or area.

When applying the compounds of this invention, amount of application can change in a wide in range scope.The agent of reactive compound It is universal and favourable that amount/amount of application treatment in accordance with the present invention method, which apply,.

The processing of-plant parts, such as blade (processing of leaf)：From 0.1~10,000g/ha, preferably 10~1,000g/ha, More preferably 50~300g/ha；If carrying out immersion liquid or instillation processing, dosage can even reduce, especially when using inert substance During such as asbestos or perlite；

The processing of-seed：2~200g is used per 100kg seeds, preferably uses 3~150g per 100kg seeds, more preferably 2.5~25g is used per 100kg seeds, most preferably uses 2.5~12.5g per 100kg seeds；

The processing of-soil：0.1~10,000g/ha, preferably 1~5,000g/ha.

The dosage of the present invention is provided as the illustrative examples of the method for the present invention.Should in one skilled in the art Adjustment application dosage is known how, especially in accordance with the property of pending plant or crop.

Combination can be used in a period of time after treatment according to the present invention, resist pest and/or plant pathogenic Fungi and/or microorganism, to protect plant.Plant produces the time range of protection after with combined treatment, and usual span is 1 ~28 days, preferably 1~14 day, more preferably 1~10 day, even more preferably 1~7 day, or propagulum material is after treatment Guard time scope may be up to 200 days.

Embodiment

Following embodiments are used to illustrate the present invention, but are not limited to the scope of the present invention.

Embodiment A potatos are tested

Potato Cultivars are Xia Bodi, plant the black earth (pH8.35, using the content of zinc in soil in Gansu zinc-deficiency 0.34 μ g/g, organic matter 8.86%)：Height above sea level 2050m, 16.4 DEG C~19.7 DEG C, rainfall 18mm~43.3mm of mean temperature, and And the relative humidity 34.5%~67.8% between June~September.Each processing has four repetitions.Each piece of region area 100m²Have 500 mounds.Reference product uses the fungicide 80%WP Mancozebs that in the market can be bought.Soil does not contaminate Disease.Field management is similar in processing procedure：On April 28th, 2010 is sowed, ridging on June 25th, 2010；Irrigate and divide three times Not in May 6, June 19, August 12 days, each 50L；Respectively weeding twice is carried out in June 9 and July 23.Prior to seeding With 210kg/ha (NH₄)H₂PO₃、750kg/ha Ca(H₂PO₄)·H₂O and 165kg/ha CO (NH₂)₂Amount of application fertilising.

The influence lifted after harvest with regard to Propineb to potato tubers nutritional quality is assessed.The sample each handled It is to randomly select the detection that 2-3 stem tuber carries out nutritional quality to laboratory.

The detection of soluble powder protein content is carried out with Coomassie brilliant G-250 method：By 0.5g Potato Tuber Tissues The grinding in water (2mL), and be homogenized in 6mL water.Homogenate centrifuges 10min under 4 DEG C, 4000rpm, and supernatant is used as solubility The source of albumen.Then dyed with Coomassie brilliant G-250.Absorbance is surveyed under 595nm.Protein content μ g/g fresh weights (FW) represent.

Content of starch assay method：25g Potato Tuber Tissues are ground and dry at 80 DEG C.Take 500mg samples 80 10min is extracted with 80% ethanol and remove supernatant at DEG C.Residue keeps 3h with will be all with 2%HCl processing, in boiling water bath Starch Conversion saccharogenesis.Sugared content can then be detected to correspond to content of starch.Ba(OH)₂Solution is used for making albumen precipitation.Phenol Phthalein is used as indicator, and 5%ZnSO₄It is slowly added to until supernatant reddens.Content of starch is represented with μ g/g fresh weights (FW).

Dry matter assay method：Each fresh sample takes 1000g potato tubers, is dried in 200 DEG C of baking ovens to perseverance Weight.The amount of dry matter is represented with mass percent.

The detection method of Zn content：With reference to national (P.R.China) standard：Measure (the GB/T 5009.14- of Zinc in Foods 2003,ICS 67.040 C 53)。

* the number of days of DAS=after planting

The Tuber yields and nutritional quality measured after harvest

^[1]It is top:The weight of single stem tuber>150g

^[2]Level-one:The weight of single stem tuber>100g

^[3]Two level:The weight of single stem tuber>50g

Soluble protein, content of starch, Zn content and the dry matter of the potato tubers handled with safe and sound raw fungicide, phase Than untreated control and the practice of peasant, show significantly to increase.

Embodiment B rice tests (field trial)

Rice varieties raise excellent No. 6 of II, are planted in 40m²Region application rate be 22.5kg/ha (each processing 3 repetitions). The safe and sound raw fungicide 70WP (Propineb 700g/kg) that can be then bought with the market and zinc fertilizer ZnSO₄Processing.

Field management is conventional and similar in processing procedure：What is planted before is winter crops rape, and rice is 2010 Sowing on June 15, in.(the NH of 300kg/ha is pressed prior to seeding₄)₂PO₃, 150kg/ha KCl and 375kg/ha CO (NH₂)₂Apply Dosage is applied fertilizer.

Influence of the Propineb to the Zn content in the grain of rice, is assessed under the field condition of China.Each processing with Machine chooses the measure that 500g samples carry out Zn content to laboratory.

^[1]A:40 DAS (number of days after planting), B:52 DAS,C:66 DAS

Zn content in the leaf and grain that are measured after harvest

, can in Rice Leaf and grain compared to untreated control and the practice of peasant when being handled with safe and sound raw fungicide The content of dissolubility zinc shows to significantly increase.

Embodiment C corn trials (field trial)

Corn variety is No. 2 rich, is planted in 80m²Region application rate for 60kg/ha, (each processing has three weights It is multiple), this experiment is located at the experiment station of Tianjin Plant Protection Institute.The type of soil is the black earth of pH7.5, content of organics 7.54%.The safe and sound raw fungicide 70WP (Propineb 700g/kg) that can be then bought with the market and zinc fertilizer ZnSO₄At implementation Reason.

Field management is conventional and similar in processing procedure：What is planted before is winter wheat, and corn was June 22 in 2010 Day sowing；Irrigated three times respectively in stem elongation phase, heading stage and pustulation period.(the NH of 225kg/ha is pressed prior to seeding₄)₂PO₃, 150kg/ha KCl and 150kg/ha CO (NH₂)₂Amount of application applies fertilizer.

Influence of the Propineb to the Zn content in niblet, is assessed under the field condition of China.Each processing Randomly select the measure that 1000g samples carry out Zn content to laboratory.

The detection method of Zn content：With reference to national (People's Republic of China (PRC)) food security standard：The measure of Zinc in Foods (GB/T 5009.14-2003, are promulgated by Ministry of Health of the People's Republic of China, 67.040 C 53 of ICS).

^[1]A:30 DAS (number of days after planting), B:54 DAS

Zn content in the leaf and seed that are measured after harvest

, can in maize leaves and seed compared to untreated control and the practice of peasant when being handled with safe and sound raw fungicide The content of dissolubility zinc shows to significantly increase.

Embodiment D rice tests (field trial)

Rice varieties are single round-grained rice Jiahua-1, are planted in 30m²Region application rate for 30kg/ha, (each processing has four Repeat).Safe and sound raw fungicide 70WP (Propineb 700g/kg) implementation that can be then bought with the market is handled.

The influence that Propineb lifts nutritional quality in the grain of rice, is assessed under the field condition of China.Each place Reason randomly selects the detection that 1000g samples carry out nutritional quality to laboratory.

Fiber content detection method：With reference to national (People's Republic of China (PRC)) food security standard：Cellulose in food Measure (GB/T 5515.5-2008, are promulgated by Ministry of Health of the People's Republic of China, 67.040 C 53 of ICS).

The detection method of crude protein content：With reference to national (People's Republic of China (PRC)) food security standard：Albumen in food The measure of matter (GB/5009.5-2010, is promulgated by Ministry of Health of the People's Republic of China, 67.040 C 53 of ICS).

Vitamin B₁Detection method：With reference to national (People's Republic of China (PRC)) food security standard：To infant, milk and Dairy produce vitamin B₁Measure (GB/5413.11-2010, is promulgated by Ministry of Health of the People's Republic of China, 67.040 C of ICS 53)。

Vitamin B₂Detection method：With reference to national (People's Republic of China (PRC)) food security standard：To infant, milk and Dairy produce vitamin B₂Measure (GB/5413.12-2010, is promulgated by Ministry of Health of the People's Republic of China, 67.040 C of ICS 53)。

The measure of iron content：With reference to national (People's Republic of China (PRC)) food security standard：The survey of iron in food, magnesium, manganese Fixed (GB/T 5009.90-2003, are promulgated by Ministry of Health of the People's Republic of China, 67.040 C 53 of ICS).

^[1]A:40 DAS (number of days after planting), B:52 DAS

The nutritional quality measured after harvest

When being handled with safe and sound raw fungicide, compared to untreated control and the practice of peasant, crude protein, dimension are given birth in the grain of rice Plain B1 and vitamin B2 show to significantly increase.

Embodiment E corn trials (field trial)

Corn variety is epoch No. 1, and the region application rate planted in 50m2 is that (each processing has four weights to 45kg/ha It is multiple).Safe and sound raw fungicide 70WP (Propineb 700g/kg) implementation that can be then bought with the market is handled.

The influence that Propineb lifts niblet nutritional quality, is assessed under the field condition of China.Each place Reason randomly selects the detection that 1000g samples carry out nutritional quality to laboratory.

The detection method of content of vitamin E：With reference to national (People's Republic of China (PRC)) food security standard：Infant is eaten Product, milk and dairy produce vitamin A. D. E measure (GB/T 5413.9-2010, are promulgated by Ministry of Health of the People's Republic of China, ICS 67.040 C 53)。

Vitamin B₁Detection method：With reference to national (People's Republic of China (PRC)) food security standard：To infant, milk And dairy produce vitamin B₁Measure (GB/5413.11-2010, is promulgated by Ministry of Health of the People's Republic of China, ICS 67.040 C 53)。

Vitamin B₂Detection method：With reference to national (People's Republic of China (PRC)) food security standard：To infant, milk And dairy produce vitamin B₂Measure (GB/5413.12-2010, is promulgated by Ministry of Health of the People's Republic of China, ICS 67.040 C 53)。

^[1]A:32 DAS (number of days after planting), B:55 DAS

The nutritional quality measured after harvest

When being handled with safe and sound raw fungicide, compared to untreated control and the practice of peasant, crude protein, dimension in niblet Raw element E, vitamin B₁And vitamin B₂Show to significantly increase.

Embodiment F grape experiments (field trial)

Apply Propineb to the product in vineyard and the improvement of vegetation to verify, apply safe and sound raw sterilization with being with or without Agent 70%WP carries out the contrast of some grape varieties.

Method

Test and carry out (Vinha Grande de Algeruz [VGA] in different vineyaries；Vinha das Faias [VF]；Vinha do Desembargador [VD] and Quinta dos Cistus [QC]), it is all located at Lisbon south about 40km, in Sai Tubaer peninsulas region, in different type and different types of soil, (sandy soil and clay-chalk, Zn content are 1.1-0.5ppm).Grape rootstock is 41B (QC) and 1103- Henry Paulsons (in other places).The age in vineyard was at 1 year (QC) To between 20 years (VG).

Table 1：Grape variety, positioned at the vineyard in Sai Tubaer peninsulas region and feature

Kind	Vineyard	Soil types	Age (year)	pH
					Dynaudio	VGA	Sandy soil	20	5.8
My tribute is sharp	VGA	Sandy soil	20	5.8
					It is domestic it is how auspicious plus	VGA	Sandy soil	20	5.8

Kind	Vineyard	Soil types	Age (year)	pH
					Pinot Blanc	VGA	Sandy soil	20	5.8
Sauvignon Blanc	VGA	Sandy soil	20	5.8
					Luo Leiluo	VGA	Sandy soil	20	5.8
Barrow carminum	VGA	Sandy soil	20	5.8
					Card is difficult to understand red	VGA	Sandy soil	20	5.8
Frank Duo Ruijia	VGA	Sandy soil	20	5.8
					Hila	VGA	Sandy soil	20	5.8
Draw Te Linjia Mount Tais	VF	Sandy soil	7	4.9
					A Ruitu	VF	Sandy soil	7	4.9
Theresa Weld sieve	VF	Sandy soil	5	4.9
					Frank Duo Ruijia	VF	Sandy soil	5	4.9
Hila	VD	Clay-chalk	8	8.1
					Cabernet Sauvignon	VD	Clay-chalk	8	8.1
Wei Ounie	QC	Clay-chalk	1	8.3
					Lapie adds	QC	Clay-chalk	1	8.3
Moscato	QC	Clay-chalk	1	8.3

Applied by using knapsack sprayer.The volume of water 322 between 355L/ha, and concentration need to adjust with Ensure fixed dosage.

Processing and untreated region are all made of (12 plants) totally 2 pieces of regions by 6 plants of plants.

In treatment region 4 applications have been carried out using the safe and sound raw fungicide 70%WP of 2.5kg/ha.In growth phase Between BBCH 77 to 85, these applications are implemented with maximum exposure face.

Table 2：Grape variety, vineyard and the application time in the certain growth stage

Sampled in 18/08 pair of blade, carry out the analysis of leaf to assess the content of zinc.

During harvest, micro wine production is all carried out to untreated and processing region.Then, grape wine be submitted into One sensory testing of row.Although having occurred that evolution in terms of the evaluation of grape wine, sense organ taste is still evaluation Portugal The decisive and critical factor of grape wine.There is no the organoleptic feature that a kind of single chemical analysis can determine grape wine, therefore, Sense organ taste is still irreplaceable.

Conclusion：

The analysis of leaf：

Table 3：Zn content mg/kg

Analysis performs (Unidade de by INIAde Ambiente e Recursos Naturais) (internal standard method LAP.PL12 VD (2009/09/20)

Sense organ taste

Sense organ taste is carried out by 4 professional tasters more than 20 years experiences, they are not aware that the grape that will be tasted Wine.Grape wine is numbered and arranged at random.Arrange to taste (Cover, 1936) for the different of couple stimulation, taster is by face To the grape wine of processing and untreated areas, and select one he more have a preference for.

From upper table, we can be observed according to grape variety as a result, or our analysis is absorbed in application or not Apply the influence of safe and sound raw fungicide, observe X²Distribution.N is equal to total trial test number, X₁It is that the trial test for applying safe and sound raw fungicide is praised Into grape wine number, X₂It is the favorable grape wine number of trial test for not applying safe and sound raw fungicide.

X²=[(| X₁-X₂|)-1]²/N

It is that we obtain the result is that X²=8.1, that is to say, that mode of the hobby of taster with applying safe and sound raw fungicide 5% level of signifiance is reached.

Claims

1. purposes of the Propineb in vitamin content in improving rice and corn, wherein amount of application are following：For plant portion The amount of the processing of position is 0.1~10,000g/ha, and the amount for the processing of seed is every 100kg 2~200g of seed, and for soil The amount of the processing of earth is 0.1~10,000g/ha.

2. purposes according to claim 1, it is characterised in that the Propineb has Fungicidally active.

3. purposes according to claim 1, it is characterised in that compared with untreated each check plant, the vitamin Content improves at least 5%, 10%, 25%, 50% or more.

4. purposes according to claim 1, it is characterised in that the dosage for improving vitamin content is following：

Amount for the processing of plant parts is 10~1,000g/ha, the amount for the processing of seed be per 100kg seeds 3~ 150g, and be 1~5,000g/ha for the amount of the processing of soil.

5. purposes according to claim 1, it is characterised in that the vitamin is vitamin B₁, vitamin B₂And vitamin E。