TWI334884B

TWI334884B - Anti-bacterial vaccine compositions

Info

Publication number: TWI334884B
Application number: TW097148939A
Authority: TW
Inventors: E Lowery David; E Fuller Troy; J Kennedy Michael
Original assignee: Upjohn Co
Priority date: 2001-03-15
Filing date: 2002-02-08
Publication date: 2010-12-21
Also published as: CY1112014T1; US7476391B2; US20050003512A1; CA2852293A1; EP1368456B1; AR071888A2; PE20021008A1; AU2002240033B2; DE60239318D1; TWI328035B; PT1368456E; JP2011097938A; TW201040262A; MXPA03008296A; WO2002075507B1; TW200916577A; TWI335934B; US20040110268A1; WO2002075507A2; TWI335935B

Description

1334884 九、發明說明：. 【發明所屬之技術領域】本申請案是2000年4月6曰建檔之美國專利申請案第 09/545,199號的部份接續申請案，其聲言1999年9月10曰建檔之美國臨時專利申請案第60/153,453號和1999年4月9曰建檔之60/128,689號的益處。本發明通常係關於確認引起巴斯德桿菌科（Pasteurellaceae) 細菌之毒力的基因，藉此容許產製可用於疫苗之新穎的減毒突變種品系，並確認瞄準毒力基因及其產物的新穎抗-病毒劑6 【先前技術】巴斯德桿菌科包括數種感染各種動物的重要病原。除了敗血性巴斯德桿菌（P. multocida)之外，該科的著名成員包括溶血性巴斯德桿菌（Pasteurella (Mannheimia) haemolytica)、胸膜肺炎放線桿菌（Actinobacillus pleuropneumoniae)和睡眠嗜血·桿菌 (Haemophilus somnus)。敗血性巴斯德桿菌是革蘭氏陰性、非活動性的球桿菌，其在許多野生動物和家畜的正常菌叢中找到，並已知在全世界的許多動物物種中引起疾病 [Biberstein在 M, Kilian，W. Frederickson和 E.L. Biberstein(編輯），Haemophilus, Pasteurella和 Actinobacillus. Academic Press, London，第61-73頁（1981)中]。在感染之後顯露的疾病包括敗血症、支氣管肺炎、鼻炎和創傷感染[在She wen等人，在 C.L. Gyles和 C.O. Thoen(編輯），Pathogenesis of Bacterial 136852.doc

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Infections in Aflimals. Iowa State University Press，Ames，第 216-225頁（1993)中回顧，以引用的方式併入本文中被敗血性巴斯德桿菌感染，通常起因於在緊迫期間的侵入，但亦可藉著空氣或接觸傳染，或經由蚤和蝨媒介者傳染。在家禽中，敗血性巴斯德桿菌感染引起急性至極急性的敗血症，在與過度擁擠、產卵、換羽或劇烈氣候改變有關的緊迫條件下，在馴養的火雞和野生水禽中是特別普遍的在牛在感染之後有類似的出血性敗血症，並顯露出包括高燒和抑鬱的病況，通常接著是很快地死亡。最可能是經由空氣接觸傳染，但亦可在明顯氣候變化的期間發生感木。在兔子，感染引起再發的化膿性鼻炎，通常接著是結膜炎、中耳炎、鼻竇炎、皮下膿腫和慢性支氣管肺炎。在嚴重感染時’兔子因為急性纖維蛋白性支氣管肺炎、敗血症或内毒素血症而致死。通常在緊迫期間產生疾病狀〜在猪貝太血性巴斯德桿菌之疾病狀態包括萎縮性鼻炎和細菌性肺炎。亦在狗、描、山羊和綿羊中檢測到類似的肺火病況。通常在許多動物的口腔菌叢中檢測到敗血性巴斯德桿菌’並因此通常是咬傷和抓傷的污染物。敗金性巴斯德桿菌品系通常是藉著英膜▲清群和菌體血清，來命名。藉著特徵性的熱_穩定抗原的表現，來區別5 個英膜群（A、B、D、Ε和〇和個菌體血清型。大多數的品系是宿主專—的，並很、感染一或兩種以上的動物。不同血清型的存在，提出關於接種疫苗的問題，因為傳統的完整死細胞細菌，通常僅提供血清型_專一的保 136852.doc 1334884 護。然而’已經證貪以一種血清型的自然感染，可導致對抗多種血清型的免疫學保護[Shewen等人，在C L Gyles和 C.O，Thoen(編輯）’ path〇genesis 0f Bacterial Infections in Animals. Iowa State University Press, Ames 第 216-225 頁（1993) 中]’且亦可藉著使用在活體内生長的失活細菌，刺激交叉保護[Rimler 等人，Am J Vet Res. 42:2117-2121 (1981)]。已經利用一種活的自然突變種敗血性巴斯德桿菌品系作為疫备’並已經顯示刺激強的免疫反應[Davis，p〇uitry Digest. 20:430-434(1987)，Schlink等人，Avian Dis. 31(1):13-21 (1987)]。然而’已經顯示如果接受者受到緊迫，該減毒品系便恢復成有毒力的狀態，或引起死亡[Davis，p〇ultry Digest· 20:430- 434 (1987)，Schlink等人 ’ Avian Dis. 31(1):13-21 (1987)]。巴斯德桿菌科的其他成員，胸膜肺炎放線桿菌，對豬顯不出嚴格的宿主專一性，且是高傳染性豬胸膜肺炎的病原體。感染通常產生激烈的出血病況，並相信藉著直接傳染的方式發生。該疾病經常是致命的，且結果在豬產業上導致嚴重的經濟損失。胸膜肺炎放線桿菌感染可以是慢性或急性的’而感染的特徵為出血、壞死性支氣管肺炎，伴隨有纖維蛋白性胸膜炎。迄今，已經將細菌毒力歸因於結構蛋白質’包括也清型-專一的莢膜多醣類、脂多醣和表面蛋白質’以及細胞外的溶胞毒素。不管這些毒力因子的純化作用，以及在許多情況下的選殖，仍不瞭解在胸膜肺炎放線桿菌感染中，這些毒力因素的正確角色。以在莢膜多醣類中之抗原差異，以及細胞外毒素的產製 136852.doc iS] 1334884 為基礎’已經確認出12個血清型的胸膜肺炎放線桿菌。血清型1、5和7與在美國的胸膜肺炎放線桿菌感染最有關聯，而血清型1、2、5、7和9則盛行於歐洲。有至少3個重要的胸膜肺炎放線桿菌細胞外毒素，其為溶血素 (Haemolysin)家族的成員，並被稱為RTX毒素。RTX毒素由許多革蘭氏陰性細菌產製，包括大腸桿菌（E. c〇li)、普通變形桿菌（Proteus vulgarisa)和溶血性巴斯德桿菌，而蛋白質通常共享結構和功能特徵。然而，得自各種血清型的毒素之差異在於宿主專一性、標乾細胞和生物學活性。主要的胸膜肺炎放線桿菌RTX毒素包括ΑρχΙ、ΑρχΙΙ和 ApxIII。ΑρχΙ和ΑρχΙΙ具有溶血活性，而ΑρχΙ是較有效的。ApxIII顯示無溶血_活性，但對肺泡巨嗟細胞和嗤中性白血球是胞毒性的。大多數的胸膜肺炎放線桿菌血清型產生這二種毒素中的兩種。例如，血清型1、5、9和11表現 ΑρχΙ和ΑρχΙΙ，而血清型2、3、4、6和8表現ΑρχΙΙ和 ApxIII。然而血清型1〇僅產生ΑρχΙ，而血清型7和12僅表現ΑρχΙΙ。產生ΑρχΙ和ΑρχΙΙ的那些胸膜肺炎放線桿菌血清型是最強毒力品系的細菌。在老鼠模式和使用隨機突變之野外型細菌感染豬的實驗中’ 5登實Αρχ毒素是毒力因子[Tascon等人’ Mol. Microbiol. 14:207-216 (1994)]。亦已經利用瞄準的突變生成作用，使編碼ΑορΑ外膜毒力蛋白質的基因失活，產生其他的胸膜肺炎放線桿菌突變種[Mulks和Buysee，Gene 165:61-66 (1995)]。 I36852.doc t S1 1334884 在溶血性巴斯德桿菌，巴斯德桿菌科物種中，已經證實至少 11種血清型（1 ' 2、5·9、12-14和 16) [Angen等人，Vet Microbiol 65(4):283-90 (1999)]，其為在新生的、斷奶的、生長中的和成年的羔羊、犢牛和山羊中爆發嚴重急性肺炎的原因[Ackermann 等人，Microbes Infect 2(9):1079-88 (2000)]。運輸、病毒感染、過度擁擠和其他的緊迫狀況，使動物易罹患溶血性巴斯德桿菌感染[Ackermann等人，在前]。咸相信溶血性巴斯德桿菌的害白金球毒素（Lkt)在發病原理中扮演重要的角色，引起細胞溶解和細胞凋零，其導致牛運輸熱的肺臟病理學特徵[Highlander等人，Infect

Immun 68(7):3916-22 (2000)]，以及在牛肺炎性巴斯德桿菌症中的肺臟傷害[Jeyaseelan 等人，Microb Pathog 30(2):59- 69 (20()1)]。Lkt是形成-孔隙的外毒素，其具有僅在反芻動物白血球和血小板中誘導細胞溶解的獨特性質[Jeyaseelan 等人’在前]。許多細胞類型的細胞溶解，是由花生四烯酸（AA)調節’而其藉著磷酸脂酶的產製作用則是由G_蛋白質-偶聯受體調節[Jeyaseelan等人（2001)，在前]。目前的研究指出溶血性巴斯德桿菌Lkt與牛CD 18，所有β2整合素的八同亞單元結合[Jeyaseelan等人，Infect Immun 68(1):72-9 (2000)]。亦已經顯示LFA-1是Lkt受體，與LFA-1結合之Lkt 並非標乾細胞專一的，Lkt與牛LFA-1結合，與鈣升高和細胞溶解有關’且牛LFA-1表現與Lkt_誘導之標靶細胞細胞 ’谷解的荨級有關[Jeyaseelan等人，Infect Immun 68(1):72-9 (2000)] 〇 136852.doc ί S3 -10- 1334884 當企圖產製疫苗組合物時，傳統的完整死細胞細菌僅提供血清型-專一的保護[Maclnnes和Smart在前]，然而，已經證實利用高毒力的血清型自然感染，可刺激對多種血清型的強保護性免疫[Nielsen，Nord Vet Med. 3 1:407-13 (1979), Nielsen, Nord Vet Med. 36:221-234(1984), Nielsen, Can J Vet Res. 29:580-582(1988), Nielsen, ACTA Vet Scand. 15:80-89(1994)]。一種已限定之活-減毒疫苗品系，產生失活形式的ΑρχΙΙ毒素’已經顯示可望對豬提供交叉保護 [Prideaux等人，Infection & Immunity 67:1962-1966 (1999)]，而其他未限定之活-減毒突變種亦已經顯示承諾[Inzana等人，Infect Immun. 61:1682-6,（1993)，Paltineanu等人，在 International Pig Veterinary Society，1992，第 214 頁中， Utrera荨人，在International Pig Veterinary Society，1992, 第213頁中]。因為與疫苗調配物有關的問題包括具有未限定、自然突變的細菌，故在此項技藝中需要合理地建構活的減毒細菌品系，以便在疫苗中使用，其將安全地刺激對抗同種或異種之巴斯德桿菌血清型的保護性免疫^尚需要確認減毒的細菌品系和細菌毒力所需的基因。藉此有助於確認抗'細菌劑之方法的發展。【發明内容】叙而5 ’本發明提供產製和使用包括減毒之革蘭氏陰性細菌的疫苗組合物的材料和方法。一方面，本發明之疫苗組合物包括在細菌之巴斯德桿菌科中的減毒物種，其為 136852.doc Γ 1334884 在此項技藝中已知的，且一部份描述在De whir st等人，j

Bacteriol. 174:2002-2013 (1992)中，全部以引用的方式併入本文中。在該科中的物種，包括但不限於放線桿菌病伴隨放線才干函（A. actinomycetemcomitans)、莢膜放線桿菌（a capsulatus、馬放線桿菌（A equun)、木狀放線桿菌（a lignieresii)、胸膜肺炎放線桿菌（胸膜肺炎嗜血桿菌 pleuropneumoniae))、精子放線桿菌（A seminis)、豬放線桿菌（A. suis)(豬嗜血桿菌（Η· suis))、瑨放線桿菌 ureae)(瑨巴斯德桿菌（p. ureae))、莢膜放線桿菌、比斯卡德分類11 (Bisgaard taxon 11)、埃及嗜血桿菌（H aegyptius)、嗜沫嗜血桿菌（H. aphr〇philus)、嗜沫嗜血^旱菌 (H. aphrophihis)(副流感嗜血桿菌（H parainnuenzae))、杜克雷氏嗜血桿菌（Η· ducreyi)、嗜血紅蛋白嗜血桿菌（H. haemoglobinophilus)、溶血性嗜血桿菌（H haem〇lyticus)、流行性感i嗜血桿菌（H. influenzae)、副随道嗜血桿菌（η paracuniculus)、副雞嗜血桿菌（H paragalHnarum)、副溶血性嗜血桿菌（H. parahaemolyticus)、副流感嗜血桿菌、副嗜沫嗜灰桿菌（H. paraphrophilus)、副嗜沫溶血性嗜灰桿菌 (H. paraphr〇haem〇lytiCUS)、副嗜沫嗜血桿菌、副豬嗜血桿菌（H” parasuis)、副豬嗜血桿菌第5型、賽格尼斯嗜血桿菌 (H. segnis)、睡眠嗜血桿菌、嗜血桿菌屬（Haem〇phUus)最 J群、嗜血♦干菌分類C(Haemophilus taxon C)、產氣巴斯德桿菌（P. aerogenes)、安納提斯巴斯德桿菌（p anatis) '禽巴斯德杯菌（P_ avium)(禽嗜血桿菌（η. avium))、犬巴斯德桿 136852.doc •12· 1334884 菌（P. canis)、·德格曼提斯巴斯德桿菌（ρ· dagmatis)、雞巴斯德桿菌（Ρ· gallinarum)、溶血性巴斯德桿菌、繭蜜糖巴斯德桿菌（P. trehalosi)((溶血性巴斯德桿菌生物型τ》、蘭給巴斯德桿菌（P. langaa)、敗血性巴斯德桿菌、侵肺巴斯德桿菌（P. pneumotropica)、口炎巴斯德桿菌（P stomatis)、佛倫提巴斯德桿菌（P. v〇lantium)(副流感嗜血桿菌）、佛倫提巴斯德桿菌、巴斯德桿菌A種、巴斯德桿菌B種和副嗜沐溶血性嗜血桿菌。較佳的是，疫苗組合物包括減毒的溶血性巴斯德桿菌、胸膜肺炎放線桿菌、睡眠嗜血桿菌或敗也性巴斯德桿菌的細菌。在最佳的具體實施例中，本發明之疫苗組合物包括減毒的敗血性巴斯德桿菌和胸膜肺炎放線桿菌之細菌品系。本發明的一項觀點，提供在以序列識別：1、3、7、9、 11 ' 13、15、17、19、21、23、25、29、31、33、37、 39、41、51、53、55、57、58、60、68、70、72、74、 76 、 78 、 80 、 82 、 84 、 100 、 1〇2 、 104 、 106 、 108 、 110 、 112 、 114 、 116 、 118 、 120 、 122 、 124 、 126 、 128 、 130 、 132 、 134 、 135 、 136 、 138 、 140 、 142 、 144 、 146 、 148 、 150 、 152 、 154 、 156 、 158 、 160 、 162 、 163 、 164 、 166 、 168、170、172和174號中任一個代表的基因序列中，含有功能突變的革蘭氏陰性細菌生物，或其物種同系物，其中該突變抑制或廢止了編碼基因產物（也就是由該基因編碼之多肽）的表現及/或生物活性；該功能突變導致細菌品系的減弱毒力。調節（也就是增加或減少）基因產物之表現及/ 13 136852.doc L S1 ⑴4884 :生物活性的功能突變’包括在中，哎在i咅·不身之蛋白質編碼區次在負責或涉及基因表現之控制除。刪除突變種包括其中刪除㈣入或刪那此士卩或部份特定基因序列的二。亦企圖使組合物，較佳的突轡釦法主ΛΑ f # 疋疫田、.且合物，包括經過佐#^的革蘭氏陰性細菌生物’可視需要包括適當的或在藥學上可接受的稀釋劑或載劑。為了修改品舉使其在疫苗調配物中是有效的，、士、圭 T疋有^的’減毒必須明顯得足以防

=病原誘發嚴㈣臨床症狀，但亦不重要得足以容許細菌在宿主中有限地複製和生長。本發明亦提供多核苷酸，其編碼在革蘭氏陰性細菌中對毒力而言必要的基因產物。本發明之多核芬酸包括DNA，像是互補DNA、基因組DNA，包括互補或反_義DNA，以及全部或部份合成的DNA; RNA，包括有意義和反義股；以及例如在Corey，TIBTECH 15:224-229(1997)中描述的肽核酸》本發明之毒力基因多核茹酸，包括在序列識別：

1、3、7、9、11、13、15、17、19、21、23、25、29、 31、33、37、39、41、51、53、55、57、58、60、68、 70、72、74、76、78、80、82、84、100、102、104 ' 106 、 108 、 110 、 112 、 114 ' 116 、 118 、 120 、 122 、 124 、 126 ' 128 、 130 、 132 、 134 、 135 、 136 、 138 、 140 、 142 、 144 、 146 、 148 、 150 、 152 、 154 、 156 、 158 、 160 、 162 、 163 ' 164、166、168、170、172和 174號中陳述的那些，或其物種同系物，編碼由序列識別：1、3、7、9、11、 13、15 ' 17、19、21、23、25、29、31、33、37、39、 136852.doc -14- 1334884 41、51、53、·55、57、58、60、68、70、72、74、76、 78、80、82、84、100、102、104、1〇6、ΐ〇8、no、 112 、 114 ' 116 、 118 、 120 、 122 、 124 、 126 、 128 、 130 、 132 、 134 、 135 、 136 、 138 、 140 、 142 、 144 、 146 、 148 、 150 、 152 ' 154 ' 156 、 158 、 160 、 162 、 163 、 164 、 166 、 168、170、172和174號之多核苷酸’或其物種同系物編碼之毒力基因產物的多核牮酸，以及在中等或高度嚴格的條件下，與在序列識別：1 ' 3、7 、9、 11、13 、15 、17、 19、 21、 23 ' 25 '29 、31、 33 ' 37、 39 ' 41 '51 ' 53、 55 ' 57 ' 58、60 '68 '70、 72 > 74、 76、78 、80 、82、 84、 100、 ‘ 102 、 104 、 106、 108、 110 、112、 114、 116、 118、 120 、122、 124 > 126、 128、 130 、132、 134、 135、 136、 138 、140、 142、 144、 146、 • 148 、150、 152 > 154、 156、 158 、160、 162、 163 > 164 > • 166 、168、 170、 172和 174號中陳述之任一個多核甞酸，或其物種同系物之非編

碼股（或互補物）雜交的多核：y：酸》因此，本發明包括得自在序列識別：1、3、7、9、11、13、15、17、19、21、 23、25、29、31、33、37、39、41、51、53、55、57、 58、60、68、70、72、74、76、78、80、82、84、100、 102、 1〇4、 1〇6、 108、 11〇、 112、 114、 116、 118、 120、 122 、 124 、 126 、 128 、 130 、 132 、 134 、 135 、 136 、 138 、 140 、 142 、 144 、 146 、 148 、 150 、 152 、 154 、 156 、 158 、 160、162 ' 163、164、166、168、170、172和 174號中陳述之巴斯德桿菌科的基因序列，以及得自其他革蘭氏陰性 136852.doc IS1 -15- 1334884 細菌生物的相.關基因序列，包括其天然存在（也就是物種同系物）和人為誘導的變體。本發明亦包括編碼從在序列識別：1、3、7、9、11、13、15、17、19、21、23、25、 29、31、33、37、39、41、51、53、55、57、58、6〇、 68、70、72、74、76、78、80、82、84、1〇〇、1〇2、 104' 106' 108' 110' 112>

114 、 116 、 118 、 120 、 122 、 134 、 135 、 136 、 138 、 140 、 152 、 154 、 156 、 158 、 160 、 124 、 126 ' 128 ' 130 、 132 、 142 、 144 、 146 、 148 、 150 、 164' 166、168、170、172和174號中陳述之任一個多核甞酸，及其物種同系物推衍之多肽的多核站酸。本發明之多核站酸的序列知識，得以迅速地獲得該多核誓酸的每個可能的片段。因此本發明提供本發明之多核苷酸的片段。

本發明更包含包括本發明之多核#酸的表現構築體。亦包括以本發明之多核苷酸轉化、轉移感染或電穿透的宿主細胞。本發明提供產製由本發明之多核莊酸編碼之多肽的方法」包括使本發明之宿主細胞在容許，且最好促進由該多核#酸編碼之基因產物表現的條件下生長，並從該宿主細胞或其生長的培養基令分離基因產物的步驟。確認本㈣之乡核料，㈣間得編碼的乡肽。本發月之夕肽。括王長和片段，或截短的蛋白質·其變體·融合或喪合型蛋白質；以及類似顯似物包括其令已經將保留性胺基酸置換導入野外型多月士中& r玉夕肽中的那些。亦提供專一地認出本發明之多肚的抗體，並包括i 亚匕栝早株和多株抗體、單鏈抗體、嵌合型抗體、人類化抗體、^ ^ ^ ^ ^ 八顯抗體和互補性決定區 I36852.doc • 16- 1334884 (CDR)-移植的抗體，以及包括專一地認出本發明之多肽的 CDR序列的化合物。本發明亦提供對本發明之抗體具有免疫專一性的抗-遺傳性型抗體。根據本發明另一項觀點，提供確認新穎的抗-細菌劑的方法’該新穎的抗_細菌劑調節革蘭氏陰性細菌毒力基因的功旎，或基因產物。本發明之方法，包括針對干擾由在序列識別：1、3、7、9、11、13、15、17、19、21、23、 25、29、31、33、37、39、41、51、53、55、57、58、 60 、 68 、 70 、 72 、 74 、 76 、 78 、 80 、 82 、 84 、 100 、 102 、 104 、 106 、 108 、 110 、 112 、 114 、 116 、 118 、 120 ' 122 、 124 、 126 、 128 、 130 、 132 、 134 、 135 、 136 、 138 、 140 、 142 、 144 、 146 、 148 、 150 、 152 、 154 、 156 、 158 、 160 、 162、163、164、166、168、170、172和 174號之任一個中陳述之DNA序列，或其物種同系物編碼的毒力基因產物表現的能力，來篩選可能的製劑，或針對干擾全部或部份由在序列識別：1、3、7、9、11、13、15、17、19、21、 23、25、29、31、33、37、39、41、51、53、55、57、 58 、 60 、 68 、 70 、 72 、 74 、 76 、 78 、 80 、 82 、 84 、 100 、 102、 104、 106、 108' 110、 112、 114、 116、 118、 120、 122 、 124 、 126 、 128 、 130 、 132 、 134 、 135 、 136 、 138 、 140 、 142 、 144 、 146 、 148 、 150 、 152 、 154 、 156 、 158 、 160、162、163、164、166、168、170、172和 174號之任一個中陳述之DNA序列，其物種同系物，或其互補股編碼的細菌基因產物之生物功能的能力，來篩選可能的製劑， 136852.doc - 17- ί S1 1334884 接著確認在這類篩選測定中提供正面結果的製劑。特定而言，干擾毒力基因產物表現的製劑包括反_義多核站酸，以及與該毒力基因序列互補的核酶（rib〇Zyme)。本發明更包括經由使用寡核苷酸-指揮之三股螺旋形成，調節本發明之基因產物轉錄的方法。 ▲干擾毒力基因產物之功能的製冑，包括毒力基因產物的變體、毒力基因產物的結合夥伴，以及這類結合夥伴的變體，和酵素抑制劑（其中產物為酵素）。提供藉著在本文中描述之方法確認出的新穎抗·細菌劑，以及治療罹患革蘭氏陰性細菌感染之個體的方法，其涉及以有效降低細菌存在之含量，投與這類新穎的抗-細菌劑。在考量下列的發明詳細說明時，對熟諳此藝者而言，本發明許多額外的觀點和益處將變得更明顯，它將描述其製備之具體實施例。

【實施方式】在本文中使用的#力基因”，為其功能或產物，對於成 :地在宿主動物中建立及/或維持細菌感染而言是必要基因。因此，毒力其^ _ 土因及/或藉此編碼之蛋白質，涉主生物的發病原理，但對生長可能不是必要的。在本文中使用的"附加簽名的突變生成作用(陶"，是以物第W〇 “"Μ51號中普遍描述的方法，以引用的方式併入本文φ，t 模式中確認毒力必要之細菌二你1如在菌企症之老鼠要之細萄基因的方法。在該方法中，使 136852.doc 1334884

用轉因子整合·’產製在基因組中分別具有隨機突變的細菌品系；每個插入突變帶有不同的DNA簽名標籤，其容許彼此區別突變種。標籤包括4〇個鹼基對的可變中心區，位在 20個鹼基對之不變”臂"的側面，其容許藉著聚合酶連鎖反應（PCR)共同-擴大中心部份。在微量滴定盤上裝配附加標籤的突變種品系，然後混合形成”接種物集合,，，以供感染研究使用。在接種之後的適當時間，從動物中分離細菌，並集合形成"回收集合"。分別擴大、標示在回收集合中的標籤和在接種物集合中的標籤，然後用來探測濾紙，其已帶有在接種物中代表突變種之所有不同標籤排成的陣列。具有減弱毒力的突變種品系，是不能從被感染動物中回收的那t也就疋說’帶有當以得自接種物集合之標籤探測時，提供雜交信號，但當以得自回收集合之標籤標籤探測時，不提供信號之標籤的品系。在該方法的變化中，可使用非·放射性的檢測方法，像是化學發光。

附加簽名之突變生成作用容許在單一的動物中，就毒力的喪失’同時篩選大量的插入突變種品系。篩選突變種敗血性巴斯德桿菌品系的19個集合，結果確認出6〇個以上降低毒力的品系，藉著後續決定個別突變種的約略叫。，證實其中許多在毒力上是減弱的。筛選胸臈肺炎放線桿菌突 :種二結果確認出超過100個品系，其在35個不同的基因有大變作用。在這些之中’在22個基因中的突變作用，產生明顯減毒的胸膜肺炎放線桿m藉著定序兩股，並推街編碼的胺基酸序列，判定藉著㈣插^瓦解了開 I36852.doc is] 19 1334884 放編閱架構的核苷酸序列。藉著將該序列與DNA和蛋白質資料庫序列相比較’定出新奇的多核苷酸和胺基酸序列兩者。在這些物種中毒力基因的知識，容許確認在溶血性巴斯德桿菌中的物種同系物。細菌’更特定而言是敗A性巴斯德桿菌、胸膜肺炎放線桿菌和溶血性巴斯德桿菌之毒力基因的確認，提供了顯示出降低毒力的微生物（也就是減毒品系），其在疫苗中是有 Φ 用的。這類微生物包括含有至少一個功能突變，使由序列識別：1、3、7、9、11、13、15、17、19、21、23、25、 29、31、33、37、39、41、51、53、55、57、58、60、 68、70、72、74、76、78、80、82、84、100、1〇2、 1〇4 、 106 、 108 、 110 、 112 、 114 、 116 、 118 、 12〇、 122 ' 124 、 126 、 128 、 130 ' 132 、 134 、 135 、 136 、 138 、 140 、 M2、 144、 146、 148、 15〇、 152、 154、 156、 158、 16〇、 162、163、164、166、168、17〇、m和 174號中任一個代 • 表之基因失活的巴斯德桿菌科突變種。熟諳此藝者將瞭解 "功能突變"可發生在本發明之基因的蛋白質編碼區，以及在調節毒力基因RNA轉錄的調節區中。熟諸此藝者亦知曉本發明之減毒的敗錄巴斯德桿菌、胸膜肺炎放線桿菌和溶血性巴斯德桿菌品系，包括帶有一個以上功能突變的那些。-個以上的突變可能導致加成或協同的減毒程度。可藉著設計來製備多個突變，或可偶缺地起因於最初企圖導入單-突變的删除事件。帶有多個删除之減毒品系的實例為鼠傷寒沙門氏桿g (saim_ua 136852.doc i s] 1334884 typhimurium)品系·，其中在功能上刪除了 cya和crp基因。該突變種鼠傷寒沙門氏桿菌已經顯示可望作為活疫苗。在敗血性巴斯德桿菌、胸臈肺炎放線桿菌和溶血性巴斯德桿菌中確認毒力基因，可提供在其他病原性物種中關於類似基因的資訊。舉例來說，an)A基因的確認，引導在多種數目的病原中，包括親水性產氣單孢菌（Aer〇m〇nas hydrophUa)、殺鮭產氣單孢菌（Aer〇m〇nas…则仏他）、鼠傷寒沙門氏桿菌、腸炎沙門氏桿菌（Salm〇neUa enteritidis)、都伯林沙門氏桿菌（Salm_Ua祕叫、雞沙門氏桿菌（Salmonella gallanerum)、百日咳球桿菌（B〇rdelia pertussis)、小腸結腸型耶爾森氏菌（加心鳴Hc〇litica)、淋病雙球菌（Neisseria gonorrh〇eae)和炭疽芽孢桿菌 (Bacillus anthracis)，確認被保留的基因。在許多的這些物種中，已經證實在aroA基因中帶有突變的減毒細菌品系，在疫苗調配上是有效的。使用在敗血性巴斯德桿菌中確認的毒力基因序列，可在其他生物中確認類似或同系的基因，特別是在巴斯德桿菌科中，以及胸膜肺炎放線桿菌、溶血性巴斯德桿菌和睡眠嗜血桿菌。同樣地，胸膜肺炎放線桿菌毒力基因的確認，可容許在其他生物中之相關基因的確認。使用敗血性巴斯德桿菌、胸膜肺炎放線桿菌和溶血性巴斯德桿菌基因作為探針的南方雜交作用，可在衍生自其他生物的染色體庫中，確認這些相關的基因。或者，在跨越物種界限的基因確認中，PCR可能是同樣有效的。作為另一種選擇，亦可使用例如敗血性巴斯德桿菌突變種 136852.doc 21 1334884 與得自其他物種之染色體庫的互補作用，來確認具有類似或相關毒力活性的基因。_，相關毒力基因的確認可引導其他生物之減毒品系的產製’其在作為其他疫苗調配物上可能是有料。已經證實存在於其他物種（例如溶血性巴斯德桿菌、胸膜肺炎放線桿菌和睡眠嗜企桿菌）中之敗血性巴斯德桿菌基因的實例，包括基因exbB、atpG、 pnp、guaB和 yjgF ° 使用STM確認的減毒敗血性巴斯德桿菌品系是插入突變種，其令已經經由將轉因子序列插入開放編閱架構或調節 DNA序列中，而使毒力基因成為無·功能的。這些插入突變物仍含有所有細菌毒力所需的遺傳資訊，並有可能藉著刪除被插入的轉因子而恢復為病原狀態。因此，在製備疫苗調配物時，#望取得蕙集自減毒品系的資訊，並創造出其中移除-#•、大部份或全部毒力基因序列的刪除突變種品系，藉此排除細菌將恢復成毒力狀態的可能性。預期使用STM確認之減毒插入突變種的疫苗特性，是與攜帶相同基因刪除之細菌的那些相同或類似的。然而，插入犬變種有可能對鄰接的基因序列，發揮"極性"的影響，而結果使插入突變種可能具有與刪除相同基因序列之突變品系不同的特徵。可使用任何在此項技藝中已熟知和慣用的許多技術，來建構刪除突變種。在一個實例中，可使用利用逆可選擇標記的策略，已經普遍地在許多細菌中使用該策略來刪除基因。關於回顧，參見例如Reyrat等人 ’ Infection and Immunity 66:4〇11_ 136852.doc -22· 1334884 4017 (1998)，以引用的常使用雙重選擇策略，方式併入本文中。在該技術中，經其中利用衍生自想要之刪除兩側的側面DNA序列，建構編碼可選擇和逆可選擇標記兩者的質體。使用可選擇標記’選擇其中以適當的位置和方式，已經將該質體整合到基因組内的適當細菌使用逆可選擇標記，選擇極少百分比之自發地排除被整合這些細菌的溶離份將僅含有想要的刪除，之質體的細菌。而無其他的外來

DNA存在。使用該技術的關鍵，是適當之逆可選擇標記的便利性》

在其他的技術中，對於DNA之指定位置重組作用使用 cre-lox系統。該系統由34個鹼基對的ι〇χ序列所組成其可被細菌ere重組酶基因認出。如果1〇χ位置以適當的方位出現在DNA中，則位在lox位置側面的序列將會被重組酶切除，結果刪除了所有的序列，除了一個1时序列的剩餘副本。使用標準重組技術，有可能在敗血性巴斯德捍菌、胸膜肺炎放線桿菌或溶血性巴斯德桿菌基因組中刪除感興趣的彳示輕基因’並以位在l〇x位置側面的可選擇標兮己 (例如編碼康黴素抗藥性的基因）置換之。cre重組酶的暫時性表現（藉著在敗血性巴斯德桿菌、胸膜肺炎放線桿菌或溶血性巴斯德桿菌中有功能之啟動基因的控制之下，含有 ere基因之自殺質體的電穿透作用），應導致1〇χ側面標記的有效排除。該過程將產生含有想要之刪除突變，和一個 ΐ〇χ序列副本的突變種。在另外的方法中，有可能以標記基因，像是綠螢光蛋白 136852.doc -23- 1334884 質（GFP)、β·半乳糖贫酶或蟲榮光素酶，直接置換在敗灰 !生巴斯U干菌、胸膜肺炎放線桿菌或溶血性巴斯德桿菌基因組中想要職的序列。在該技術t，藉著pcR製備位二想要刪除側面的DNA斷片，並選殖到敗血性巴斯德桿菌、胸膜肺炎放線桿菌或溶血性巴斯德桿菌的自殺（非·複製）載體内。在側面彳列之間選殖表現卡£，其含有纟敗灰性巴斯德桿菌、胸膜肺炎放線桿菌或溶血性巴斯德桿菌中有作用的啟動基因，以及適當的標記基因。將該質體導入野外型敗血性巴斯德桿菌、胸膜肺炎放線桿菌或溶血性巴斯德桿菌内。分離併入並表現該標記基因（或許以極低之頻率）的細菌，並檢查適當的重組事件（也就是以該標記基因置換野外型基因）。可藉著將其投與易感受的動物，來證實這些生物降低的毒力，及其免疫原性。雖然有可能單獨投與本發明之無毒力的微生物，但最好是以含有適當佐劑（們）和在藥學上可接受之稀釋劑（們）或載劑（們）的疫苗組合物之形式，投與或多個這類突變種微生物。就與本發明之無毒力微生物可相容的意義而言，載劑（們）必須是"可接受的"，且對待免疫個體而言是無害的。載劑通常是水或生理鹽水，其將疋無菌和無熱原的。待免疫個體是需要保護，使其免於由敗血性巴斯德桿菌、胸膜肺炎放線桿菌、溶企性巴斯德桿菌或其他病原性微生物之毒力形式引起之疾病的個體。應瞭解本發明之疫苗可用於人類醫學和獸醫醫學的範疇中因此，待免疫之個體可以是人類或其他動物，例如農 136852.doc •24· 场動物’包括牛、'綿羊、豬、馬、山羊和禽類（例如雞、火雞鴨和鵝）’同伴動物，像是狗和！结；外來及/或動物圓的動物；以及實驗動&，包括老鼠、大鼠、兔子、天竺鼠和倉鼠。本發明亦提供敗血性巴斯德桿菌、胸膜肺炎放線桿菌或溶血性巴斯德桿菌毒力所需之多肽和相對應的多核苷酸。本發明包括天然存在和非_天然存在的多核苷酸及其多肽產物兩者。天然存在的毒力產物，包括各種基因和多肽物種，以及在敗血性巴斯德桿菌、胸膜肺炎放線桿菌或溶血性巴斯德桿菌以外之生物中表現的相對應之物種同系物。非-天然存在的毒力產物，包括天然存在之產物的變體，像是類似物，以及包括共價修改的毒力產物。在較佳的具體實施例中’本發明提供包括在序列識別：1、3、7、9、 11 ' 13、 15 > 17 、19 、21、 23、 25 ' 29、31 '33 '37 39 ' 41、 51 '53 '55 、57、 58 ' 60、 68 ' 70 '72 '74 Ί6、 78、 80 '82 、84、 100、 102 、104 、106、 108、 110 % 112 、114 、 116、 118、 120、 122 、124 、126、 128、 130 132 、134 135、 136 > 138 ' 140 ' 142 ' 144、 146、 148 150 、152 ·> 154、 156、 158 ' 160 、162 、163、 164 ' 166 % 168 、170 Λ 172和 174號中陳述之, 字列的毒力多核苷i 酸，及其物種同系物，以及具有由該多核嘗酸編碼之胺基酸序列的多肽·。本發明提供編碼細菌毒力基因產物之新穎的經過分離和純化的敗血性巴斯德桿菌、胸膜肺炎放線桿菌和溶血 136852.doc -25- !生巴斯德桿菌多核铝酸（例如DNA序列和RNA轉錄本，兩者的有意義和互補反義股）。本發明之Dna序列包括基因組和cDNA序列，以及全然或部份以化學方式合成的DNA 序列。本發明之基因組DNA，包括本發明之多肽的蛋白質編碼區’並包括可在相同物種之其他細菌品系中找到的變體。在本文中使用並在此項技藝中已瞭解的"合成"，意指與酵素方法相對，純粹在化學上產製多核钻酸的方法。因此’ ”全然”合成的DNA序列是全部藉著化學方式產製的，而"部份"合成的DNAs則包括其中所得的DNA中只有部份是由化學方式產製的那些。編碼敗血性巴斯德桿菌毒力基因產物的較佳DNA序列，是在序列識別：1、3、7、9、 11、13、15、17、19、21、23、25、29、31、33、37、 39、41、51、53、55、57、58、60、68、70、72、74、 76 ' 78、80、82、84、100、102、104、106、108、110、 112、114、116、118和120號中陳述的，及其物種同系物。編碼毒力基因產物之較佳的胸膜肺炎放線桿菌Dna序列’是在序列識別：122、124、126、128、130、132、 134 、 135 、 136 、 138 、 140 、 142 、 144 、 146 、 148 、 150 、 152、154、156、158、160、162、163和 164號中陳述的，及其物種同系物。較佳的溶血性巴斯德桿菌毒力基因產物，是在序列識別號：166、168、170、172和174號中陳述的，及其物種同系物。熟諳此藝者將迅速地瞭解本發明的較佳DNA，包括雙股的分子，例如具有在序列識別： 1、3、7、9、11、13、15、17、19、21、23、25、29、 136852.doc •26- 1334884 31、33、37、39、41、51、53、55、57、58、60、68、 70、72、74、76、78、80、82、84、100、102、104、 106 ' 108 、 110 、 112 、 114 、 116 、 118 、 120 、 122 、 124 、 126 、 128 、 130 、 132 、 134 ' 135 ' 136 、 138 、 140 、 142 、 144 、 146 、 148 、 150 、 152 、 154 、 156 、 158 、 160 、 162 、

163、164、166、168、170、172和 174號中陳述之序列，及其物種同系物的分子，根據DNA的Watson-Crick鹼基配對規則，連同具有可從序列識別：1、3、7、9、11、13、 15 ' 17、19、21、23、25、29、31、33、37、39、41、 51 ' 53、55、57 ' 58、60、68、70、72、74、76、78、 80、82、84、100、1〇2、104、106、108、110、112、 114 、 116 、 118 、 120 、 122 、 124 、 126 、 128 、 130 、 132 、 134 、 135 、 136 、 138 、 140 、 142 、 144 、 146 、 148 、 150 、 1 C Λ

“ ' 154 、 156 、 158 、 160 、 162 、 163 、 164 、 166 、 168 、 170 ' 172和174號之序列推衍之序列的互補分子（"非-編碼股"或"互補物"）。較佳的還有編碼由序列識別：i、3、7、 9、11 ' 13 ' 15、17、19、21、23、25、29、31、33、 37 ' 39、41、5卜 53、55、57、58、60、68、70、72、 74 ' 76、78、80、82、84、100、102、104、106、108、 110、 112、 114、 116、 118、 120、 122、 124、 126、 128、 13〇、 132 、 134 、 135 、 136 、 138 、 140 、 142 、 144 、 146 、 148 、 150 、 152 、 154 、 156 、 158 、 160 、 162 、 163 、 164 、 166、168、170、172和174號中陳述之任一個多核牮酸，及其物種同系物編碼之基因產物的多核：y：酸。本發明尚包 136852.doc 1S1 -27- 括敗血性巴斯德桿菌、胸膜肺炎放線桿菌和溶血性巴斯德桿菌DNA的物種（最好是細菌）同系物。由本發明k供之多核β酸序列資訊’使得有可能藉著已熟知的技術’包括南方及/或北方雜交作用，和聚合酶連鎖反應（PCR)，確認和分離編碼相關細菌毒力分子的多核苷酸。相關之多核甞酸的實例，包括編碼與由序列識別： 1 ' 3、7 、9、11 '13 ' 15 ' 17、 19、 21、23 、25 、29、 31、 33 ' 37、39 、41 ' 51、 53 ' 55、 57、58 '60 、68 ' 70、 Ί1、 74 ' 76 '78 、80、 82 '84、 100、 102、 104、 106 、108 ' 110、 112、 114、 116 、118 、120、 122、 124、 126 、128 ' 130、 132、 134 > 135 ' 136 ' 138 ' 140、 142 > 144 ' 146 、148、 150、 152、 154 ' 156 、158、 160、 162、 163 、164 '166' 168、 170、 172和 174 •號中陳述之任一個多核茹酸 ’及其物種同系物編碼之毒力基因產物同種之多肽，以及共享本發明之毒力基因產物1 的一或多個生物及/ 或物理特性’在結構上相關之多肽的多核苷酸〇本發明亦包括編碼細菌基因產物之DNA序列，其在中等或南度嚴格的條件下，與在序列識別：1、3、7、9、11、 13、15 ' 17、19、21、23、25 ' 29、31、33、37、39 ' 41、51 ' 53、5S、57、58 ' 60、68、70、72、74、76 ' 78 ' 80 ' 82、84、1〇〇、1〇2、104、106、108、110、 112 ' 114、116 ' 118、和 12〇、122、124、126、128、 130 、 132 、 134 、 135 、 136 、 138 、 140 、 142 、 144 、 146 、 148 、 150 、 152 、 154 、 156 、 158 、 160 、 162 、 163 、 164 、 136852.doc 1334884 166 168、170、172和174號中陳述之任一個多核苷酸，及其物種同系物的非'編碼股或互補物雜交。其將與編碼毒力多肽的DNA序列雜交，但本發明企圖包括遺傳密碼的簡併。代表性的高度嚴格條件，包括在65。〇至75。〇下以包括〇·2Χ SSC/G.l% SDS之緩衝溶液的最後沖洗，而代表性的中等嚴格條件，包括在35。〇至45。〇下以包括U SSC/0.1% SDS之緩衝溶液的最後沖洗。應瞭解在此項技藝中，可按照在 Ausubel 等人（編輯），Pr〇t〇c〇ls in

Biology，John Wiley & Sons (1994)第6 〇 3至6 4 1〇頁中的描述，經由改變溫度和緩衝溶液，或鹽濃度，達到相等嚴格度的條件。可憑經驗決定，或以探針之烏嘌呤/胞嘧啶 (GC)鹼基配對的長度和百分比為基礎，精確地計算在雜交條件中的修改。可按照在Sambrook等人（編輯），MoUcuUr Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press: Cold Spring Harbor, New York (1989), 第9.47至9.51頁申的描述，計算雜交條件。

亦提供併入毒力基因序列的自動複製之重組表現構築體’像是質體和病毒DNA載體。亦提供表現構築體，其中編碼毒力多肽的多核甞酸，以可操作之方式與内源或外源的表現控制DNA序列和轉錄終止序列連接。可藉著pcR選殖毒力基因’使用敗血性巴斯德桿菌基因組DNA作為模板。為了易於將基因插入表現載體内，選擇PCR引子，使知PCR-擴大之基因’在5'端在開始密碼子之前，具有限制酵素位置’並在3’端在終止密碼子tag、TGA或ΤΑ A 136852.doc -29- 1334884 之後，具有限制酵素位署。^ # 置右需要，可根據Grosjean和

Fiers，Gene，18:199-209 (Ί_、 „ . (2) ’ 以及 Konigsberg和 Godson,

Proc. Natl. Acad Sci me a、Λ • (USA)，80:687-691(1983)描述的大腸才干函岔碼子優先權，改變美田击μ — s 7 ^'愚因中的畨碼子，但不改變胺基酸。使用密碼子的最優化作用，彳導致在大腸桿菌中產製時增加基因產物的表現。如果以細胞外的方式產製基因產物，在大腸桿菌或其他細菌的細胞質中，或進入細胞培

養基中’則選殖該基因，不堂甘― +而其開始畨碼子，並放在表現載體内信號序列之後。

根據本發明的另一項觀點，提供宿主細胞，包括使用本發明之多核#酸序列，以容許本發明之毒力多肽表現的方式，穩定或暫時地轉化、轉移感染或電穿透的原核生物和真核生物細胞。本發明之表現系統包括細菌、酵母菌、真菌、病毒、無脊椎動物和哺乳動物細胞系統。本發明之宿主細胞是用來發展專—地與毒力基因產物產生免疫反應之抗體的有價值之免疫原來源。本發明之宿主細胞顯然可用在大規模產製毒力多肽的方法中，其中使細胞生長在適當的培養基中，並從該細胞或從細胞生長於其中的培養基中’藉著例如免疫親和力純化作用，或任何此項技藝中已热知並慣用的眾多純化技術，分離想要的多肽產物。可使用任何適當的宿主細胞，像是大腸桿菌，其他的細菌，包括敗血性巴斯德桿菌、桿菌屬(BaciUus)和金黃色葡萄球菌 (s. au娜），酵母菌，包括巴斯德畢赤酵母菌（piehia p祕七) 和酒酵母菌（SaCCharomyces cerevisiae)，昆蟲細胞或哺乳動 136852.doc -30- 1334884 物細胞，包括CHO細胞，利用此項技藝中已知的適當載體，來表現基因產物。可直接產製蛋白質，或與肽或多肽融合’以細胞内或細胞外之方式產製，藉著分泌至細菌細胞的近膜間隙内’或進入細胞培養基中。蛋白質的分泌需要信號肽（亦稱為前-序列）；已知許多得自原核生物和真核生物的信號序列，對於重組蛋白質之分泌是有功能的。在蛋白質分泌製程期間’藉著信號肽酶移除信號肽，產生成熟的蛋白質。欲簡化蛋白質純化製程’可在基因密碼序列的5·或3,端附加純化標籤。常用的純化標籤包括一片6個組胺酸殘基 (美國專利第5,284,933號和5,310,663號）、由Schmidt和 Skerra，Protein Engineering，6:109_122(1993)描述的鏈黴菌抗生物素蛋白·親和力標籤、FLAG肽[Hopp等人，Biotechnology， 6:1205-1210 (1988)]、縠胱甘肽 S-轉移酶[Smith 和 Johnson， Gene，67:3M0 (1988)]，以及硫氧化還原蛋白[LaVallie等人， • Bl〇/TeChn〇l〇gy，丨1:187·^ (1993)]。欲移除這些肽或多肽，可在融合接合處插入蛋白水解切開認知位置。常用的蛋白酶為因子Xa、凝血酶和腸激酶。本發明亦提供由本發明之多核苷酸編碼的經過純化和分之敗ik丨生巴斯德桿菌、胸臈肺炎放線桿菌和溶血性巴斯德桿菌毒力多肽。目前較佳的是包括由在序列識別：i、、9、11、13、15、17、19、21、23、25、29、31、 33、37、39、41、51、53、55、57、58、60、68、70、 72、74、76、78、80、82、84、1〇〇、1〇2 ' 1〇4、ι06、 136852.doc i S] 31 1334884 108、 110、 112、 114、 116、 118、 120、 122、 m、 126、 128 、 130 、 132 、 134 、 135 、 136 ' 138 、 140 、 142 、 144 、 146 、 148 、 150 、 152 、 154 、 156 、 158 、 160 、 164 、 166 、 168、170、172和174號中陳述之任一個多核苷酸，及其物種同系物編碼之胺基酸序列的多肽。本發明包括由選自包括下列之DNA編碼的毒力多肽：a)在序列識別：1、3、 7 、 9 、 11 ' 13 、 15 、 17 、 19 、 21 、 23 、 25 、 29 、 31 、 33 、

37、39、41、51、53、55、57、58、60、68、70、72、 74、76、78、80、82、84、100、102、104 ' 1〇6、1〇8、 110、 112、 114、 116、 118、 120、 122、 124、 126、 128、 130 、 132 、 134 、 135 、 136 、 138 、 140 、 142 、 144 、 146 、 148 、 150 、 152 、 154 、 156 、 158 、 160 、 164 、 166 、 168 、 170、172和174號之任一個中陳述的DNA序列，及其物種同系物；b)編碼由序列識別：1、3、7、9、11、13、15、

17、19、21、23、25、29、31、33、37、39、41、51、53、 55、57、58、60、68、70、72、74、76、78、80、82、84、 100、102、104、106、1〇8、110、112、114、116、118、120、 122 、 124 、 126 、 128 、 130 、 132 、 134 、 135 、 136 、 138 、 140 、 142 、 144 、 146 、 148 、 150 、 152 、 154 、 156 、 158 、 160 、 164 、 160、168、170、172和174號中任一個，及其物種同系物編碼之敗血性巴斯德桿菌、胸膜肺炎放線桿菌或溶血性巴斯德桿菌多肽的DNA分子；以及c)編碼毒力基因產物之DNA 分子’其在中等嚴格的條件下與（a)或（b)之DNA雜交。本發明亦包括與本發明之較佳多肽，具有至少大約 136852.doc 1S1 -32- 1334884

99。/❶、至少大約95:至少大約9〇%、至少大約85%、至少大約議、至少大約75%、至少大約7〇%、至少大約洲、至v大約60/〇、至少大約55〇/〇和至少大約同一性及/或同種性的多肽。關於本發明之較佳多肤，在本文中將胺基酸序列"同-性"百分比定義為在將兩個序列排成一直線並導入間隙（若需要）’以便達到最大序列同一性百分比之後’在候選序列中，與在毒力基因產物序列中之殘基相同的胺基酸殘基的百分比，但不將任何保留性置換視為序列同一性的一部份。關於本發明之較佳多肽，在本文中將序列同種性"百分比定義為在將序列們排成一直線並導入間隙（若需要），以便達到最大序列同一性百分比之後在候選序列中與在毒力夕肽序列中之殘基相同的胺基酸殘基的百分比，且亦將任何保留性置換視為序列同一性的—部份。可按照在表八和3中的陳述，定義保留性置換。

表A

保留性置換I

胺基酸 GAP ILV CSTM NQ DE KR HFWY NQDE 側鏈特徵脂肪族無-極性極性-不帶電極性-帶電荷芳香族其他可從天然的細菌細胞來源中分離本發明之多肽，或可以化子方式合成，但較佳的是藉著涉及本發明之宿主細胞的 136852.doc •33- 重組程序產製。·本發明之毒力基因產物可以是全長的多月太具有生物活性的片&，或其保留特有之生物或免疫學 ’舌丨生的變體。變體可包括毒力多肽類似物，其中刪除或取代或多個指定之（也就是天然編碼的）胺基酸，或其中加入一或多個非·指定的胺基酸：（1)不喪失一或多個該毒力基因產物特有的生物活性或免疫學特徵；或使該毒力基因產物特殊的失物活性失去能力。刪除變體亦企圖包括缺乏對生物活性並非必要之部份多肽的片段，而插入變體包括融合多肽，其中野外型多肽或其片段已經與其他多肽融合。體毒力多肽包括其中已經藉著修改編碼本發明之多肽的多核铝酸，而導入保留性置換的那些。在此項技藝中承認的保留性置換，根據其相關的物理特性將胺基酸分類，並可按照在表A(得自W0 97/09433，第10頁，1997年3月13 日公告（PCT/GB96/02197 ’ 9/6/96建檔））中的陳述定義。或者’可按照在Lehninger，[Biochemistry，第 2版；Worth Publishers, Inc· NY:NY (1975)，第 71-77頁]中的定義將保留性胺基酸分組’如同在表B中的陳述。

表B

保留性置換II

側鏈特徵胺基酸無-極性(忌水性的） A.脂肪族的： ALIVP B.芳香族的： FW C.含硫的： Μ D.介於兩者間難以確定的： G 不帶電-極性的 A.羥基： STY I36852.doc -34 B. 醯胺： · C. 硫風基： D. 介於兩者間難以確定的

NQ C G 帶正電(驗性的）：帶負電(酸性的）：

本發明之變體毒力產物包括成熟的毒力基因產物，也就是其中移除前導或信號序列，具有額外的胺基終端殘基》期待在-1位置處具有額外之甲硫胺酸殘基的毒力基因產物’還有在位置-2和-1處具有額外之甲硫胺酸和離胺酸的毒力產物。這些類型的變體，對於在細菌細胞類型中的重組蛋白質產製是特別有用的。本發明之變體亦包括其中已經導入衍生自其他蛋白質之胺基終端序列的基因產物，以及包括未曾在天然存在之蛋白質中發現的胺基終端序列的變體。本發明亦包括具有起因於使用獨特表現系統之額外胺基酉文殘基的變體多肽。例如，使用市售的載體，其以帶有穀胱甘肽-S-轉移酶（GST)之融合蛋白質的形式表現想要的多肽’在從想要的多肽中切開GST組份之後，提供了在位置_ 1處具有額外之甘胺酸殘基的想要多肽。亦期待使用其他載體系統表現而產生的變體。一的"一詞，表示本發明之抗體的可變區獨佔地認得並與毒力多肽結合(也就是能夠區別單一毒力多肽與相關的毒本發明亦包括抗體（例如單株和多株抗體、單鏈抗體、嵌合型抗體、人類化、人類的和CDR-移植的抗體，包括含有專一地認得本發明之多肽的CDR序列的化合物），以及對f力基因產物或其片段專一的其他結合蛋白質。"專 136852.doc •35· 1334884 力多肽’不管在多’肽家族中發現的序列同一性、同種性或類似性）’但亦可經由與抗體可變區之外側序列的交互作用’特別是在該分子之恆定區中，與其他蛋白質產生交互作用（例如在ELIS A技術中，金黃色葡萄球菌蛋白質a或其他抗體）。決定本發明抗體之結合專一性的篩選測定，是此項技藝中已熟知並慣用的。關於這類測定的總括性討論’參見Harlow 等人（編輯），Antibodies A Laboratory Manual; Cold Spring Harbor Laboratory； Cold Spring Harbor，NY (1988)，第6章。亦期待認得並與本發明之毒力多肽片段結合的抗體’其限制條件為該抗體，如同上文定義，是第一優先對從其中衍生該片段的本發明之毒力多月太專一的。由本發明提供之DNA和胺基酸序列資訊，亦使得有可能有系統地分析毒力基因及其編碼之基因產物的結構和功月b。關於編碼本發明之毒力基因產物的多核芬酸之知識，亦可能獲得反-義多核苷酸，其認得並與編碼本發明之毒力多肽的多核甞酸雜交。提供全長和片段的反_義多核甞酸。熟諸此藝者將知曉本發明之片段的反·義分子包括⑴ 專一地認出並與特異RNA雜交的那些（藉著比較編碼本發明之毒力多肽的DNA與編碼其他已知分子之DNA的序列來決定），以及（ii)認出並與編碼毒力蛋白質家族之變體的 RNA雜交的那些。與編碼蛋白質毒力家族之其他成員的 RNA雜交的反義多核甞酸，亦可經由序列比較來確認，確認分子家族序列的特徵或簽名。 136852.doc ^ IS] 1334884 本發明更期待經由核酶的使用，調節基因表現的方法β 關於回顧，參見Gibson*Shillitoe，Mol.Bi〇tech.7:125· 137 (1997)。可使用核酶技術，以序列專一之方式，經由 ⑴互補RNA與標靶mRNA的雜交作用，以及（ii)經由互補股固有的核酸酶（nuclease)活性’切開已雜交的mRNA，抑制 mRNA的轉譯。可藉著經驗法則確認核酶，但更佳的是以在標靶mRNA上的可進入位置為基礎而特別設計的 [Bramlage等人 ’ Trends in Biotech 16:434-438 (1998)]。可使用此項技藝中已熟知並慣用的外源或内源之遞送技術，完成將核酶遞送至標靶細胞。外源的遞送方法可包括使用瞄準微脂粒，或直接局部注射。内源的方法包括使用病毒載體和非-病毒質體。當將核酶設計成與編碼毒力基因產物之多核苷酸的特有區域互補時，其可專一地調節毒力基因的表現。因此，專一地調節"企圖意指本發明之核酶僅認得單一的多核苷酸。同樣地，亦可將核酶設計成調節所有或一些蛋白質家族的表現。設計該類型的核酶，使其認得在所有或一些編碼蛋白質家族的多核㈣中，受到保護的多核#酸序列。本發明肖包括經由使用寡核#酸_指揮之三股螺旋形成作用’調節本發明之毒力基因轉錄的方法。關於回顧參見 Lavrovsky等人，Biochem M〇1 Med 62:ii22 (Η”）。，用序列專-的寡核#酸，按照在WwCdek模式中的定義’其在主凹溝（maj〇r groove)中與雙股DNA雜交，完成三股螺旋形成作用。序列專—之寡核答酸的雜交作用几 136852.doc •37· 隨後調節DNA-結合蛋白質（包括例如轉錄因子和聚合酶）的舌丨生。對雜交作用而言，較佳的標靶序列包括轉錄調節區，其調節毒力基因產物的表現。能夠形成三股螺旋的寡核站酸，亦可用於標靶DNA序列的特定位置之共價修改作用。將可用於共價修改作用的寡核：y：酸與各種傷害DNa之製劑偶聯，如同在Lavrovsky等人[在前]中的描述。敗血性巴斯德桿菌、胸膜肺炎放線桿菌和溶血性巴斯德桿菌毒力基因的確認，使得該基因和基因產物可用在確認抗-細菌劑的方法中。這類方法包括測定可能之製劑，其干擾以在序列識別：1、3、7、9、11、13、1 5、17、19、 21、23、25、29、31、33、37、39、41、51、53、55、 57、58、60、68、70、72、74、76、78、80、82、84、 100、102 ' 104、106、108、110、112、114、116、118、 120 、 122 、 124 、 126 、 128 、 130 、 132 、 134 、 135 、 136 、 138 、 140 、 142 、 144 、 146 、 148 、 150 、 152 、 154 、 156 、 158 、 160 、 162 、 163 、 164 、 166 、 168 、 170 、 172和174號任一個中陳述之DNA序列，及其物種同系物物（也就是以序列識別：1、3、7、9、11、13、15、17、19、21、23、 25、29、31、33、37、39、41、51、53、55、57、58、 60 、 68 、 70 、 72 、 74 、 76 、 78 、 80 、 82 、 84 、 100 、 102 、 104' 106、 108、 110、 112、 114、 116、 118、 120、 122、 124 、 126 、 128 、 130 、 132 、 134 、 135 、 136 、 138 、 140 、 142 、 144 > 146 、 148 、 150 、 152 、 154 ' 156 、 158 、 160 、 162、163、164、166、168、Π0、172和 174號之 DNA序列 136852.doc -38- 代表的基因，其編褐毒力基因產物，或是與序列識別： 1 ' ： 3、7 、9、11 '13、 15、 17、 19 ' 21 ' 23 、25 、29、 3卜 33 ' 37、39 、41、 51、 53 ' 55、 57 > 58 '60 、68、 70、 72、 74、76 '78 、80、 82 、84 、100、 102、 104、 106 、108 、110、 112、 114、 116 、118 、120、 122、 124、 126 、128 、130、 132、 134、 135 、136 、138、 140、 142、 144 、146 、148、 150、 152、 154 、156 > 158 ' 160、 162、 1334884 163、164、166、168、170、172 和 174 號之 DNA 序列相鄰’該基因編碼毒力基因產物，或涉及毒力基因產物表現的調節）代表之毒力基因產表現的能力，或測定可能之製劑’其干擾全部或部份由在序列識別：1、3、7、9、11、 13 、 15 、 17 、 19 、 21 、 23 、 25 、 29 、 31 、 33 、 37 、 39 、 41 、 51 、 53、55、57、58、60、68、70、72、74、76、78、80、82、84、 100、102、104、1〇6、ι〇8、110、112、114、116、118、120、 122、124、126、128、130、132、134、135、136、138、140、 142、144、146、148、150、152、154、156、158、160、162、 163、164、166、168、170、172和 174號任一個中陳述之DNA 序列’其物種同系物或其互補股編碼之細菌基因產物之功能的能力’接著確認在這類測定中肯定的製劑。在這些測疋中有用的多核：y：酸和多肽，不僅包括如同本文中揭示之基因和編碼的多肽，還包括其實質上具有與野外型基因和多肽相同活性的變體。由上述方法產製的毒力基因產物，可用於高輸貫量測定中’綿選抑制劑。待篩選之可能製劑的來源為化學化合物 136852.doc m -39- 2、鏈黴菌、其仙菌和真菌的發酵培養基，以及植物和 /、他卓木的細胞卒取物。至於德亡口 , 主於帶有已知之酵素活性的蛋白 r以該活性為基礎建立測定，並就抑制該活性的能力來綿選大量可能的製劑。至於與其他蛋白質或核酸產生交互作用的蛋白質，建立結合測^，直接測量這類交互作用，並就抑制結合交互作用的能力，筛選可能的製劑。企圖根據本發明的該項觀點，使用此項技藝中已知的不同測定。當已知或藉著對已知基因產物之序列類似性而預測毒力基因產物的功能時，可在關鍵為該基因產物之功能及/或特性的酵素或其他類型之生物學及/或生化測定中，篩選可能的抑制劑。當已知或藉著對已知基因產物之序列類似性而預測毒力基因產物與其他蛋白質或核酸產生交互作用時，可直接在結合測定中篩選該交互作用的抑制劑。本發明企圖倍增測定，以便筛選和確認與毒力基因產物結合的抑制劑。在-個實例中，固定毒力基因產物，並在假疋之抑制劑化合物的存在和缺乏下，評估其與結合夥伴的交互作用。在另一個實例中，在假定之抑制劑化合物的存在和缺乏下，在溶液測定中評估在毒力基因產物與其結合夥伴之間的交互作用。在兩個測定中，降低在毒力基因產物與其結合夥伴之間的結合作用，便確認該化合物為抑制劑°在其中毒力基因產物結合夥伴為蛋白質的那些情況下，亦期待其他的測定。例如，期待二_雜化物測定的變化’其中藉著按照在1995年8月3曰公告之PCT出版物第 95/20652號中的描述，檢測在轉化或轉移感染之宿主細 136852.doc -40· 胞2衫信號’來確認蛋白f/蛋白f交互作用的抑制劑β 又到本發明期待的候選化合物，包括選自的化合物。為了確認小分子抑月括⑴化學庫、⑺天❹物庫和（3)包括隨機的肤、 =酸或有機分子的綜合庫。化學庫由已知化合物的結物’或經由天然產物筛選確認為"命中”或"指標，，的匕二物所組成。天然產物庫是微生物、動物、植物或海洋物的集合，可用來創造混合物，以便經由：⑴得自土壌、植物或海洋微生物之肉湯的發酵和萃取作用，或⑺植物或海洋线料取仙來篩選。天然產㈣“聚^ 及其衍生物、非-核糖體肽及其變體(非-天然存在的)。關於回顧’參見Seienee 282:63_68 (1998)。綜合庫是由大量的，、寡核:y：酸或有機化合物構成的混合物。它們相對上較谷易藉著傳統的自動合成方法、PCR、選殖或專利合成法來製備。特別感興趣的是肽和寡H酸綜合庫。其他亦 :興趣的庫包括肽、蛋白質、肽模仿物、多平行合成的集合、重組和多肽庫。關於綜合化學和從其中產生之庫的回參見 Myers, Curr. Opin· Biotechnol. 8:7〇i_7〇7 (I"7)。經由使用在本文中描述的各種庫來確認抑揚調節劑，容許修改候選者”命中”（或"指標"），使對調節活性"命中"的能力發揮最大效用。亦可設計其他受到本發明期待的候選抑制劑，並包括可溶形式的結合夥伴，以及嵌合或融合蛋白質形式的結合夥伴。在本文中使用的結合夥伴’廣泛地包括抗體、抗體片 136852.doc 41 1334884 段和經過修改的化合物，其包括對已確認之毒力基因的表現產物’為免疫專一的抗體功能部位。當毒力基因產物的結合夥伴（也就是配體）是未知的時，可使用其他測定’包括經由測量受試之結合夥伴與標乾蛋白質的直接結合，來確認標靶蛋白質之結合夥伴的測定，以及經由利用離子喷霧質譜分析/HPLC方法，或其他物理和分析方法的親和力超過濾作用，確認標靶蛋白質之結合 ^ 夥伴的測定。或者，可使用在Fields和Song，Nature， 340:245-246 (1989)，以及 Fields 和 Sternglanz，Trends in

Genetics，i〇:286_292 (1994)中描述的酵母菌兩個·雜化物系統，兩者均以引用的方式併入本文中，間接評估這類結合父互作用。兩個-雜化物系統是檢測在兩個蛋白質或多肽之間交互作用的遺傳測定。可用它來確認與已知的感興趣蛋白質結合的蛋白質，或描畫對該交互作用很重要的功能部位或殘基。已經發展出該方法學的變化，來選殖編碼 φ DNA·結合蛋白質之基因，確認與蛋白質結合的肽，並篩選藥物。兩個-雜化物系統係利用一對交互作用蛋白質使轉錄激活功能部位與DNA-結合功能部位非常接近的能力，後者與報告者基因的上游激活序列（UAS)結合，且通常在酵母菌中進行。該測定需要建構兩個雜化物基因，其編碼（1)與第一個蛋白質融合的〇]^八_結合功能部位，和與第二個蛋白質融合的激活功能部位。DNA_結毫功能部位使第一個雜化物蛋白質瞄準報告者基因的1;八8;然而，因為大多數的蛋白質缺少激活功能部位，該dna'结合雜 -42· 136852.doc IS1 1334884 化物蛋白質不能激活報告者基白質，A合右幻轉錄。第二個雜化物蛋表規m卜b 自己不旎激活報告者基因的表現，因為它不與UAS結合。 + …、而备兩個雜化物蛋白質句出現時，第一和第二個蛋白質、 , 貝07非共仏父互作用，將激活功能部位拾在IMS上，激活了報主n . 教活了報告者基因的轉錄。當已

力基因產物(例如第一個蛋白質)會與其他蛋白質或核酸產生交互作用時，可使用該測定來檢測干擾該結合交互作用的製劑。將不同的受試製劑加至該系統中，監視報告者基因的表現；抑制劑的出現將導致報告者信號的缺乏。。

當毒力基因產物的功能是未知的，且沒有已知與該基因產物結合的配體時’亦可使用酵母菌兩個雜化物測定來確認與該基因產物結合的蛋白質。在確認與第一個蛋白質 (標乾蛋白質）結合之蛋白質的測定中，產製大量的分別編碼不同的第二個蛋白質的雜化物基因，並在該測定中篩選。通常，由其中總cDNA或基因組DNA與激活功能部位連接的大量質體群編碼第二個蛋白質。該系統適用於各種蛋白質，且其甚至不需要知道第二個結合蛋白質的身份或功此。该系統是高度敏感的’並可檢測其他方法未透露的交互作用；即使是可誘發轉錄作用的暫時性交互作用，產生穩定的mRNA，其可重覆地轉譯，產生報告者蛋白質。可使用其他測定，搜尋與標靶蛋白質結合的製劑。_種確認受試配體與標靶蛋白質之直接結合的這類筛選方法，描述在美國專利第5,585,277號中，以引用的方式併入本文中。該方法依賴蛋白質通常以折疊和未折疊狀態的混合物 136852.doc -43· 1334884 形式存在，並在兩種狀態之間不斷交替的原理。當受試配體與折疊形式的標靶蛋白質結合時（也就是當受試配體為標靶蛋白質之配體時），與配體結合的標靶蛋白質分子維持其折疊狀態。因此，當出現與標靶蛋白質結合之受試配體時，折疊的標靶蛋白質以比在缺乏配體時更高的程度存在。可藉著任何能區別折疊和未折疊狀態之標靶蛋白質的方法’來判定配體與標靶蛋白質的結合。幾乎任何的製劑都可作為受試配體，藉著該方法來評估，包括但不限於金屬、多肽、蛋白質、脂質、多醣類、多核苷酸和小的有機分子。其他確認標靶蛋白質之配體的方法，描述在^卟〇1以等人，Anal. Chem·，69:1683-1691 (1997)中，以引用的方式併入本文中。該技術在溶液相中，就與標靶蛋白質的結合’一次篩選20-30個製劑的综合庫。藉著離心超過遽作用，從其他的庫組份中分離出與標靶蛋白質結合的製劑。接著使保留在濾紙上的特別選出之分子，從標靶蛋白質中 # .釋放，並藉著1·1151^0和氣動協助之電喷霧（離子噴霧）電離質譜分析分析之。該程序以對標靶蛋白質最高的親和力來選擇庫組份，並對小分子庫是特別有用的。在敗血性巴斯德桿菌感染的活體内老鼠模式中，就其對毒力的影響，評可利用菌血；肺炎的模式

評估藉著最初筛選確認的抑制劑/結合劑。心内膜炎、敗血性關節炎、軟組織膿腫或中。例如，可在;ί 合物之前或之後， 136852.doc * 44 - 1334884 子攻毒。對照組動物，僅投與生理鹽水代替假定之抑制劑/ 結合劑化合物，則提供標準，藉此可定出受試動物的惡化。其他的動物模式包括在Animal and Plant Health Inspection Sevice，USDA，1994 年 1 月 1 日編輯，§§113.69-113.70 ; Panciera和Corstvet，Am. J. Vet. Res. 45:2532-2537 ; Ames 等人，Can. J. Comp. Med. 49:395-400 (1984);以及Mukkur， Infection and Immunity 18:583-585 (1977)中描述的那些。干擾細菌毒力的抑制劑/結合劑可防止感染的建立，或一旦已建立感染，可逆轉其結果。在疫苗組合物中，可使用任何此項技藝中已知的佐劑，包括以油為基礎的佐劑，像是福瑞德氏（Freund's)完全佐劑和福瑞德氏不完全佐劑，以黴菌酸鹽為基礎的佐劑（例如海藻糖二黴菌酸鹽），細菌的脂多酿（LPS)，肽聚糖 (peptidoglycans)(也就是胞壁質、黏肽或糖蛋白，像是N-歐巴卡（Opaca)、胞壁醯基二肽[MDP]或MDP類似物），蛋白多糖（例如萃取自肺炎克雷伯氏桿菌（Klebsiella pneumoniae))，鏈球菌製品（例如OK432)，拜歐斯丁（Biostim)TM(例如 01K2)，歐洲專利109 942號、歐洲專利 180 564號和歐洲專利23 1 039號的"艾思康（Iscoms)"，氫氧化I呂，皂角嘗， DEAE-葡聚聽，中性油（像是米格來醇（miglyol))，植物油 (像是花生油），微脂粒，普魯尼克（Pluronic)R多元醇，瑞比（Ribi)佐劑系統（參見，例如英國專利-A-2 189 Ml)，或介白素類，特別是刺激細胞調節之免疫的那些。另一種佐劑是由厄麥可雷塔菌（Amycolata)( —種放線菌目 •45- 136852.doc i S] 1334884 (ACtin〇mycetales)中之細菌屬）的萃取物所組成，已經描述在美國專利第4’877,612號中。此外，可購f專利的佐劑混合物。所使用之佐劑，一部份將視接受者生物而定。投藥的佐劑含量，將視動物的類型和尺寸而定。可藉著例行的方法迅速地定出最適切之劑量。

疫田組合物可視需要含有疫苗-可相容的在藥學上可接受之（也就是無菌和無_毒的）液態、半固態或固態稀釋劑，其作為藥學媒劑、賦形劑或介質。可使用此項技藝中已知的任何稀釋劑。代表性的稀釋劑，包括但不限於聚氧乙烯山梨糖醇單月桂酸酯、硬脂酸鎂、羥基苯甲酸甲酯或丙酿、滑石、讓酸鹽、；殿粉、乳糖、蔬糖、右旋糖、山梨糖醇、甘露糖醇、阿拉伯樹膠、磷酸鈣、礦物油、可可脂和可可油。

亦可以便於遞送之形式包裝疫苗組合物。可將組合物封入膠囊、膠鍵（caplet)、小藥囊、亘狀膠囊、明膠、紙或其他令益中。當進入接受生物内的免疫原組合物是可相容的時二這些遞送形式是較㈣，特較在以單位劑量形式遞送該免疫原組合物時。可以例如錠劑、膠囊、栓劑或豆狀膠囊之形式包裝劑量單位。可藉著任何傳統的方法，將疫苗組合物導入待免疫的個體内’包括例藉著靜脈内、皮内、肌肉内、乳房内、腹腔内或皮下注射；藉著口服、舌下、經鼻、經肛門或經陰道遞送。處理可包括單一劑量，或在一段期間内多個劑量。本發明亦包括使用本發明之減毒細菌品系，來製造預防 136852.doc -46- 或減輕細菌感染及/或與其有關之症狀的疫苗醫藥。本發 ^亦提供使用本發明之抑制劑，來製造預防或減輕細菌感染及/或與其有關之症狀的醫藥的用途。藉著下列的實例解釋本發明。實例i描述敗血性巴斯德桿菌突變種的建構K列2係關於敗血性巴斯德桿菌突變種的篩選。實例3提出判定敗血性巴斯德桿菌突變種之毒力的方法。實例4描述敗血性巴斯德桿菌毒力基因的選殖。實例5提出在其他物種中，與敗血性巴斯德桿菌毒力基因有關之基因的確認。實例6描述胸膜肺炎放線桿菌突變種的建構。實例7提出減毒之胸臈肺炎放線桿菌突變種的篩選。實例8係關於胸膜肺炎放線桿菌毒力基因的確。實例9描述胸膜肺炎放線桿菌突變種和野外型細菌的双*爭性攻毒。實例10定出已確認之胸膜肺炎放線桿菌基因的特徵。實例11提出胸膜肺炎放線桿菌突變種保護對抗野外型細菌攻毒的效果。實例12描述在溶血性巴斯德桿菌中’物種同系物毒力基因的確認。實例1 建構附加標籤-轉因子之敗血性巴斯德桿菌突變種的庫在親代載體pLOF/Km[Herrero等人，J Bacteriol. 172:6557-67 (1990)](先前已證實其在敗血性巴斯德桿菌中是有功能但無目標的[Lee等人，Vet Microbiol· 50:143-8 (1996)])中，建構附加標籤-轉因子突變種的庫。將質體pL〇F/Km建構成為自殺載體PGP704的修改，並包括在Tac啟動基因的控制之下的轉因子酶基因’以及編碼康黴素抗藥性的迷你（mini)_ 136852.doc -47· 1334884 丁1110可置換單元。按照下述，藉著修改？1^〇?/1<：爪，使其接受含有半-隨機[NK]35序列的序列標籤，來建構質體pTEF-1。首先修改質體pLOF/Km，排除在多選殖區域中獨特的〖卩111限制位置，然後在迷你-丁1110區導入新的〖卩111位置。以ΚρηΙ消化質體，並根據製造者建議的草案，以克勒諾 (Klenow)片段填滿所得的突起末端。根據製造者建議的草案（Gibco BRL, Gaithersburg, MD和 Boehringer Mannheim, Indianapolis, IN)，進行在本文中描述的限制消化和連接作用。鈍端產物自我-連接，產生命名為pLOF/Km--ΚρηΙ的質體，為了擴大，將其轉化至大腸桿菌DH5ot^pir内。在37 °C下，在Luria-Bertani(LB)培養基中繁殖大腸桿菌 ΌΗ5α:(λρΪΓ φ 80dlacZAM15, recAl, endAl, gyrA96, thi-l, hsdR17 (rk'5 mk, supE44, relAl, deoR, Δ (lacZYA-argF) U169。使用得自 QIAGEN Inc.(Santa Clarita，CA)的 QIAGEN SpinPreps製備質體，並以Sfil消化，其切開迷你-TnlO可置換單元中的獨特位置。藉著黏接寡核苷酸 TEF1(序列識別86號）和TEF3(序列識別87號）來製備Sfil-Kpnl-Sfil接合體，並將所得的雙股接合體連接到Sfil位置内，產生質體pTEF-Ι。藉著Genosys Biotechnologies (The Woodlands，TX)合成寡核苷酸TEF1和TEF3(以及所有在本文中描述的其他寡核苷酸）。 TEF1 S'-AGGCCGGTACCGGCCGCCT 序列識另ij : 86號 TEF3 5'-CGGCCGGTACCGGCCTAGG 序歹識另: 87號如下製備在其ΚρηΙ位置内插入獨特序列標籤的pTEF-1。 -48- 136852.doc 1 S1 1334884 使用 GeneAmp XL PCR套組（PE Applied Biosystems，Foster

City，CA) ’ 在包括每種dNTP各250 μΜ，1.5 mM Mg(OAc)2，引子TEF14(序列識別：88號）和TEF15(序列識別：89號）各 100微微莫耳，1毫微克TEF26(序列識別：9〇號）作為模板 DNA和2.5單位重組Tth DNA聚合酶XL的條件下，進行 PCR，產生雙股DNA標籤。 TEF14 5，-CATGGTACCCATTCTAAC 序列識別：88號 TEF15 5'-CTAGGTACCTACAACCTC 序列識別：89號 TEF26 序列識別：90號 5，-CTAGGTACCTACAACCTCAAGCTT-[NK]35-AAGCTTGGTTAGAATGGGTACCATG 反應條件包括最初在95 °C下培養1分鐘，接著是在95 °C 下30秒，在45°C下45秒和在72°C下15秒，循環30次，接著在72°C下最後培養2分鐘。以ΚρηΙ消化PCR產物，並使用 QIAGEN 核甞酸移除套組（QIAGEN，Inc., Chatsworth, GA)，根據製造者建議的草案純化。將獨特的標籤序列連接到先前已使用標準程序，以ΚρηΙ消化，並以牛小腸鹼性鱗酸酶（Boehringer Mannheim)脫鱗酸化的直線化pTEF-Ι之迷你-Tnl 0單元内。將所得的質體庫轉化至大腸桿菌 DH5otipir 内。根據 DIG 使用者指引（Boehringer-Mannheim) 進行菌落點分析，如下進行雜交和檢測。基本上根據Genius非-放射性的使用者指引（Boehringer Mannheim Biochemicals)、DIG-PCR標示套組的產品傳單 (Boehringer Mannheim Biochemicals)，以及 CSPD的產品傳 •49- 136852.doc i S1 1334884 單（Boehringer Mannheim Biochemicals) * 進行雜交作用〇為了產製探針，建立100微升的初級PCR反應，使用

Amplitaq PCR 緩衝溶液（PE Applied Biosystems)、200 μΜ dNTPs、引子TEF5(序列識別：91號）和TEF6(序列識別： 92 號）各 140 微微莫耳、2 mM MgCl2、2.5 單位 Amplitaq(PE Applied Biosystems)和 1 毫微克質體 DNA。 TEF5 5'-TACCTACAACCTCAAGCT 序列識別：91 號 TEF6 5'-TACCCATTCTAACCAAGC 序列識別：92號循環條件包括最初在95°C下培養2分鐘，接著是在95°C 下30秒，在50°C下45秒和在72°C下15秒，循環35次，最後在 72 °C 下培養 3 分鐘。在 2%-3:1 NuSieve GTG(FMC BioProducts，Rockland，ME, USA):瓊脂糖凝膠上，使用電泳分離擴大產物，並切開和純化109個鹼基對的產物。使用QIAGEN凝膠萃取套組（QIAGEN)進行凝膠萃取作用。在50微升PCR反應中，使用DIG PCR套組、引子TEF24和 TEF25各50微微莫耳，以及帶有2 mM dNTP母液之DIG探針合成混合物的1:1混合物，標示大約1 5毫微克的初級產物。 TEF24 5'-TACCTACAACCTCAAGCTT 序列識別：93號 TFE25 5'-TACCCATTCTAACCAAGCTT 序歹4 識另: 94號 PCR條件包括最初在95°C下培養4分鐘，接著是在95°C 下30秒，在5(TC下45秒和在72。(：下15秒，循環25次’最後在72 t下培養3分鐘。在90微升的總反應體積中’以 Hindlll消化已標示的PCR產物’並從不變的引子臂中純化，使用 2%-3:1 NuSieve GTG (FMC BioProducts):填脂糖凝 -50- 136852.doc (S3 1334884 夥。切開含有已標不之可變標戴的區域，並將整個凝膠片溶解於95°C的10毫升DIG EasyHyb中1〇分鐘，並使其變性。使用 Hybond®-N+膜（Amersham-Pharmacia Biotech)製備墨點。在96孔培養盤中’使用大約3〇毫微克的pcr產物，製備每個標籤的標輕DNA。加入等體積的οι n NaOH，使試樣變性，並使用得自Schleicher和Schuell (Keene, NH USA)的Minifold ITM Dot-Blot褒置，以極少的真空，將每個試樣塗在膜上。以150微升中和溶液（〇 5 M Tris/3 Μ 他(：1，阳7.5)和150微升2乂88(：沖洗各孔。在如咖1丨111^11 (Stratagene，La Jolla, CA，USA)中，以UV使膜交聯，並在 42 °C下，在20毫升DIG EasyHyb緩衝溶液中預先雜交 (prehybridized) 1小時。加入變性的探針，並在42它下進行雜交過夜。以含有0.1% SDS的2X SSC沖洗該膜兩次，每次5分鐘。在以標準Genius檢測草案（Genius手冊）處理之刖’以50毫升預先加溫15分鐘至68 °C之含有0.1% SDS的 0.1 X SSC緩衝溶液，進行兩次高嚴格度的沖洗。為了安全、較低之成本、容易使用和減少危險物質，想要使用非-放射性的檢測系統。在最初的實驗中，使用先刖描述的類似程序[Mei等人，Mol Microbiol. 26:399-407 (1997)]，在陰性對照組中獲得無法接受的雜交背景值。為了降低背景’將標籤長度從30個鹼基對增加至總共70個，延長擴大因子使其包含所有位在可變區側面的序列，使用較低濃度的dig-dUTP，並藉著凝膠純化移除位在序列標籤區域側面的保留序列。最重要的是，在檢測得自轉因子本 136852.doc 1S1 1334884 身的背景雜交之後，使用PCR產生隊]35序列標藏，作為墨點中的標靶DNA，而非含有附加標籤之轉因子的完整質體。使料些修改，排除背景，使得化學發光/非·放射性篩選變得更有效。藉著菌落墨點，篩選大約4百個不同的轉化物，其起因於PTEF-1與PCR產生之序列標籤的連接作用，並將％個最強的雜交菌落集合在微量滴定盤内，以供進一步使用。即使重複標籤的可能性極低，亦對其他標籤探測主標籤的半個培養盤，以便證實沒有標籤重複。純化含有這些標籤的質體，並轉化至大腸桿菌S17-l:Xpir (pir，recA，thi，pro, hsd, (r-m+), RP4-2, (Tc::Mu), (Km::Tn7), [TmpR], [SmR]) 内’並在37°C下，在Luria-Bertani (LB)培養基中繁殖已經轉化的細菌。在接合交配時，使用9 6個含有附加標籤之質體pTEF-Ι的大腸桿菌S17-1 :λρίι·轉化物中的每一個，產生敗血性巴斯德桿菌的轉因子突變種。敗血性巴斯德桿菌品系丁 F5是衍生自UC6731(—種牛臨床分離物）的天然莕啶酮酸抗藥性突變種。在37t和5% C〇2中，使敗血性巴斯德桿菌品系生長在腦心浸液（BHI)培養基（Difco Laboratories， Detroit，MI，USA)上，此時在培養盤上生長。藉著使每個大腸桿菌Sl7-Upir/pTEFl:[NK]35純種系和TF5品系生長至後對數期’建立交配。將每種附加標籤之pTEF_丨純種系各50微升培養物與200微升TF5培養物混合，並將各50微升的交配混合物沾染在先前已放在含有1 〇〇 mM IPTG和10 mM MgS04之BHI盤上的0.22 TM濾紙上。在37°C和5%C02 -52- 136852.doc IS] 1334884 中培養過夜之後，將每個濾紙上的交配混合物洗到3毫升 PBS中，並各取25微升放在bhin5Gk1GG盤上。在選擇性過夜生長之後’藉著牙籤移至200微升BHIN50K50内，將菌落集人在微量滴定盤内，確定在微量滴定盤的每個孔中總是含有帶有相同序列標蕺的轉因子突變種。在過夜生長之後，在各孔中加入50微升75%甘油，並將培養盤冷凍儲存在。藉著將轉因子突變種移至微量滴定盤，聚集19個集合，確定每孔含有帶有適合該孔之標籤的轉因子突變種。換句話說，在每個微量滴定盤中的特定孔，總是含有帶有相同序列標籤的轉因子突變種，即使轉因子在那些突變種中的位置可能是不同的。實例2 減毒之敗血性巴斯德桿菌突變種的老鼠篩選使用敗血症之老鼠模式篩選19個敗血性巴斯德桿菌轉因子突變種的集合。從-8(TC儲存中移出集合之敗血性巴斯德桿菌轉因子突變種的冷凍培養盤，並藉著從每孔中移出 10微升至新的96孔圓底培養盤（Corning Costar，Cambridge， MA，USA)(含有200微升腦心浸液（DIFCO)，其帶有50微克/ 毫升萘咬酮酸（Sigma)和50微克/毫升康黴素（Sigma) (BHIn5°k5(>))中，繼代培養。在37°C和5% C02中培養該盤過夜’不需振盪。藉著從各孔中移出10微升至新的平底 96-孔培養盤（c〇rning Costar)(每孔含有1〇〇微升BHI)中，並在37°C下培養，以大約150 rpm振盪，繼代該過夜培養盤。使用微量滴定盤讀取器監視〇〇540。在大約0.2至0.25 -53- 136852.doc i S] 1334884 的OD54〇時’藉著混合從微量滴定盤每孔中各別取出的1 〇〇微升’集合各盤形成"輸入集合以BHI適當地稀釋培養物至大約104、105、l〇6CFU/毫升之劑量，並藉著腹腔内才又藥’以每種稀釋度各0.2毫升感染雌性14-16克的BALB/c 老鼠。在感染之後2天，將一或兩隻存活的老鼠安樂死，並收獲脾臟。在1.0毫升無菌的〇.9〇/0生理鹽水中，將整個脾臟均質化。製備從1〇-2至1〇-5的勻漿稀釋度，並平舖於 BHIN Κ培養盤上。在過夜生長之後，在1〇毫升βΗΙ肉湯中集合至少20,〇〇〇個菌落，形成"回收集合,，，並以35〇〇

Xg離心0.5毫升的回收集合，然後根據先前描述的草案 [Wilson，在 F.M. Ausubel 等人（編輯），Current pr〇t〇c〇丨 s in M〇1CCUlar Βί〇1οπ 第1冊 John Wiley and Sons, New York, 第2.4.1 2.4.5頁（1997)中]，使用小球來製備基因組DNA。利用有毒力之野外型敗血性巴斯德桿菌的最初實驗，指 :可從脾臟、肺臟、腎臟和肝臟回收該生物，表示真的感木之敗血症模式。按照在實例丨中的描述，進行”輸入"和，回收集合兩者的墨點，並藉著目視檢查和藉著半-定量分斤平估兩者。按照在實例1中的描述進行雜交作用，除了用得自輸入和回收集合的0.5微克基因組DNA作為模板之外。半-定量分析指出在單一純種系中是否已經發生顯著的減/。如果突變種不能在宿主中存活，此時與輸入信、較回收彳§號應該是極低的，產生高輸入/回收比例。大多數的突變種在活體内的生長像在活體外的-樣好，並因此发e & ，、15琥的比例應該大約等於丨。選擇由定量分 136852.doc -54- 1334884 析選出在回收集合中降低很多的純種系，進行進一步的研究。在目視評估由墨點製成的軟片之後，亦選擇具有可疑之輸入/回收比例的額外純種系。實例3 判定敗企性巴斯德桿菌候選突變種的毒力從原始的集合盤中分離顯示減少從脾臟回收之每個可能的突變種，並分別用在攻毒實驗中，以便證實並例行地評估由該轉因子突變引起的減毒。使得自在活體内篩選之個別候選突變種，在”艽的〗％ c〇2中，生長在綿羊血液瓊脂盤中過夜。將每種突變種大約6個菌落接種在BHI$湯中，並容許生長6小時。製備稀釋物，並按照上述分別以1〇2、 ΙΟ3' 104、l〇5CFU感染5隻老鼠。藉著在6天後，相對於野外型比較死亡率，來判定減毒，假設存活的老鼠是受到保護的’然後以超過野外型品系之ldw大約2〇〇倍的濃度，以一個劑量的野外型敗血性巴斯德桿菌攻毒。然後對每個攻毒組老鼠定出存活率。結果指出120個可能的轉因子突變種中有62個是減毒的，具有比野外型品系更高至少1〇倍的LDm近似值。在表 1中列舉純種系及其乙〇5〇近似值。亦與每個攻毒組平行地執行利用野外型品系的對照組實驗，而在野外型攻毒組中所有案例的死亡率為1 〇〇%。除了 LDw值之外，表1亦提供得自疫苗接種和攻毒實驗的數據。簡言之，以-次劑量藉著腹腔内注射，利用在表 1中出不之個別敗血性巴斯德桿菌品系疫苗接種的老鼠組 136852.doc is] •55· 1334884 (n=5至10)，比具有毒力之野外型品系的LD5〇更高大約200 倍。觀察動物28天，隨後計算其死亡率數字。表1 敗血性巴斯德桿菌毒力基因

核甞酸序列識別编號：代表性的分難物可能的基因功能疫苗接種的存活/總數攻毒的存活/絶數一野外型 - 0/10 - <10 23 PM1BI guaB 10/10, 10/10. 10/10 9/10. 9/10 4.3x106 11 PMID1 dsbB 10/10, 5/10 10/10, 5/5 8.4 x 104 3 PM1BD7 atpG 5/5, 10/10 10/10 >3x 105 74 ΡΜ1ΒΗΠ yhcJ(HIOI45) 10/10 5/10 >2x105 70 PMIBF6 yabK (ΗΠ020) 3/5, β/10 9/9 >2x105 19 PM2G8 fhaC 4/5.9/10 9/9 >4x 105 76 PM3C9 yiaO (ΗΙ0Ϊ46) 3/5 >6x 105 118 PM3GU UnkO 4/5, 10/10 10/10 >3 x 105 31 PM7B4 iroA (UnkB) 0/5 17 PM4C6 thaB (fhaB2) 2/5, 10/10. 9/10 10/10, 9/9 >3 x 106 9 PM4G10-T9 dnaA 4/5 >5x105 1 PM4D5-T5 atpB 5/5 >4x105 53 PM4D5*TI UnkC2 5/5 >4x105 15 PM4F2 maB(fhaBt) 3/5, 6/10, 10/10 6/6. 10/10 >3x 105 41 PM5F7 mrcB 4/5 1 x103 7 PM5E2 dcvB 0/5, 3/10 2/3 ND 68 PM6H5-TI xylA 5/5 >3x 105 78 PM6H8 yigF(HI0719) 5/5, 9/10 9/9 >3x105 108 PM7D12 P叩 5/5, 9/10 9/9 5\ PM8CIR1-T2 UnkCl 5/5 -6 x I05 37 PM8CI-T3 mxlB 5/5 x 105 58 PM8C1R1-T6 UnkOI 5/5 -6x105 45 PMI0H7 purF(HII207) 3/5, 8/10. 8/10 8/8, 8/8 >3x105 25 PM10H10.T2 ΗΠ501 5/5 >1x104 72 PM11G8-T2 YRiK 5/5 >2.4 x 103 21 PMI1G8-T4 grcA 5/5 >2.4 x 103 84 PM12H6 yyam (ΗΪ0687) 3/5, 0/10 -2.2 x 103 33 PM15G8-T2 kdlB 5/5 >1.2 x 105 116 PM15G8-T1 UnkK 5/5 >1.2 x 105 104 PM16G1I-T1 hmbR 3/5 >1.9 x 105 29 PM16GH-T2 hxuC 3/5 >1.9x105 35 PM16H8 iRtC 5/5. 10/10 10/10 >2.4x105 80 PM16H3 ylcA (ΗΙ00Ϊ9) 5/5, 10/10 > 2.0 x 105 49 PMI7H6-T1 sopE 4/5 *6x105 120 PM17H6 UnkP 4/5 -6x105 5 PM18F5-T8 cap5E 5/5 >2.4x105 82 PM18F5-T10 y〇jB (HI0345) 5/5 >2*4x105 13 PM19A1 exbB 5/5. 10/10 10/10 >1.2x105 112 PM19D4 rci 5/5.8/10 8/8 -1.6 x 105 39 PM20AI2 mioC (HI0669) 3/5, mo 8/8 -2x104 60 PM20C2 UnkD2 5/5. 10/10 10/10 >8.2 x 106 56· 136852.doc 1334884 實例4 選殖並確認敗血性巴斯德桿菌毒力所需之基因進一步分析已證實其為減毒的每個轉因子突變種’以便判定被瓦解之開放編閱架構的身份。擴大、純化，並以已知不能在轉因子中切開的限制酵素，消化得自每個突變種的DNA，通常產生與轉因子雜交的4-8千個鹼基之片段。使用由轉因子編碼的康黴素抗藥性來選擇，每個轉因子突變種至少選殖一個片段。使用得自pLOF/Km之已標示的18000個鹼基之Mlul片段作為探針，對每個減毒的突變種進行與多個限制酵素的南方雜交作用，確認可供選殖之適當尺寸的片段。將得自每個突變種的迷你-TnlO單元和側面的DNA選殖到PUC19 内，並使用内部引子TEF32和TEF40，引子步行（walking) ’ 而在一些案例中為萬能的pUC-19引子，定出位在侧面的序列。 TEF-32 GGCAGAGCATTACGCTGAC 序列識別：95號 TEF-40 GTACCGGCCAGGCGGCCACGCGTATTC 序列識別：96號使用得自 PE Applied Biosystems (Foster City，CA)的 BigDyeTM染料中止劑化學（Dye Terminator Chemistry)套組進行定序反應，並在ABI Prism 377 DNA定序器上執行。獲得每個純種系的假定被中斷之開放編閱架構的雙股序列。使用定序者（Sequencer) 3.0軟體（Genecodes, Corp·，Ann Arbor, MI)集合並分析序列數據。使用GCG程式[Devereux等人 ’ 1997，Wisconsin 套裝版本 9.0 ’ 9.0 版 Genetics Computer -57- 136852.doc

1 SI 1334884

Group, Inc” Madisonl，a b : 目則可獲得的資料庫中搜尋同種的序列。在3 7 %確遇已減毒的純 J ’屯種糸中，在迷你·Τη丨〇可置換單元有多個插入。分別將含* 寻匕括其側面序列的每個插入選殖到 pGP704内，並與野冰别+ 、交配，產生新的敗血性巴斯德桿菌突變種，每個僅攜帶客彻夕個原始插入中的一個。再分別測贫各個突變種，來判定成Λ、赤 ° J疋成為減毒表現型之原因的插入。藉著

定序兩個股’ U被瓦解、預期之開放編閱架構的核嘗酸序列’並使用預期的胺基酸序列，就類似序列搜尋目前可獲得的資料庫。該序列與已知的基因、未知的基因，以及先前定序之假定的開放編閱架構相配，或不與任何先前確認之序列相配。對於與先前確認之序列具有同種性的那些基因，在表1中陳述已指派之可能的功能。實例5

在其他物種中確認相關的基因在不同的實驗中，亦使用胸膜肺炎放線桿菌（App)進行 STM。一個App品系，在基因中含有插入，定序該基因（序列識別97號）’並確認其為敗血性巴斯德桿菌atp(}基因的物種同系物。該結果暗示在其他細菌物種中，存有先前未知之敗血性巴斯德桿菌基因的同系物，亦可使其突變而產生其他細菌物種的減毒品系，以供在疫苗組合物中使用。為了判定其他敗血性巴斯德桿菌基因的同系物是否存在其他的細菌物種中，對得自其他物種的基因組DNA進行南方雜交作用，使用胸膜肺炎放線桿菌atpG基因作為探針。 I36852.doc -58- 1334884 使用CTAB方法分離胸膜肺炎放線桿菌、溶血性巴斯德桿菌（Ph)、敗血性巴斯德桿菌和睡眠嗜血桿菌（Hs)基因組 DNA，並在37°C下以EcoRI和Hindlll消化2小時。在0.7%瓊脂糖凝膠上，在TEF緩衝溶液中以40伏特分離已消化的 DNA過夜。接著將凝膠浸入0.1 M HC1中30分鐘，浸入 0.5MNaOH/l·5MNaCl中兩次，各15分鐘，浸入2.5M NaCl/lM Tris，pH 7.5中兩次。使用20Χ SSC缓衝溶液（3 Μ NaCl/0,3 Μ檸檬酸鈉），將DNA移至硝基纖維素膜 (Amersham Hybond N+)上過夜。在自動交聯裝置上，使用 UV Stratalinker (120毫焦耳），使DNA與該膜交聯。使該膜在50 °C下，在5X SSC/1%封阻溶液/〇. 1%月桂醯肌胺酸鈉 /0.02% SDS中預先雜交大約7小時，然後在5〇°C下，在含有PCR產生之atgG探針的相同溶液中雜交過夜。在〇61^八111??〇11系統2400中，利用〇61^八111卩乂1?€11套組，使用引子DEL-1389(序列識別：98號）和TEF-46(序列識別：99號）來製備探針。模板為基因組胸膜肺炎放線桿菌 DNA。 DEL-1389 TCTCCATTCCCTTGCTGCGGCAGGG 序列識別：98號 TEF-46 GGAATTACAGCCGGATCCGGG 序列識別：99號利用在94°C下5分鐘的最初加熱步驟，在94°C下變性30 秒，在5(TC下黏接30秒，並在72。(：下延長3分鐘’循環30 次，以及在72°C下5分鐘的最後延伸步驟，完成PCR。在瓊脂糖凝膠上分離擴大產物，使用QIAquick凝膠純化套組 (QIAGEN)純化，並使用 DIG-High 引子套組（Boehringer ·59· 136852.doc i si 1334884

Mannheim)標示。從雜交溶液中移出墨點，並以2χ §^漂洗，並以相同的緩衝溶液沖洗兩次，每次5分鐘。然後在 60 C下以0.5Χ SSC沖洗墨點兩次，每次15分鐘。使用核酸檢測套組（Boehringer Mannheim)使同種的譜帶顯色。使用EcoRI消化的DNA，在溶血性巴斯德桿菌、睡眠嗜血桿菌和胸膜肺炎放線桿菌中檢測出單一的譜帶。使用

EcoRI消化的DNA，從敗血性巴斯德桿菌中檢測到兩個譜帶。實例6 建構附加標籤-轉因子之敗血性巴斯德桿菌突變種的庫轉因子突變生成作用，使用先前已經報告，在胸膜肺炎放線桿菌中具有功能且隨機的pL〇F/Km[Tasc〇n等人，j Bacteriol. 175:5717-22 (1993)]。欲建構胸膜肺炎放線桿菌之附加標籤轉因子突變種，在接合交配時，使用96個分別含有預先選擇之附加標籤質體（pTEF_1:[NK]35)的大腸桿菌 S 17-1. Λ pir轉化物，產生胸膜肺炎放線桿菌品系Ap225(衍生自在活體内繁殖之ATCC 27088品系的血清型1自發性莕啶酮酸抗藥性突變種）的轉因子突變種。在37和5% c〇2 中’使胸膜肺炎放線桿菌品系生長在帶有丨〇微克/毫升B_ 於驗酿胺腺嘌呤二核苷酸（vi。）（Sigma，st. Louis，

Missouri)的腦心浸液（BHI)(Difc〇 Laboratories，Detroit， MI)中’此時在培養盤上生長。在^它下在LuriaBertani (LB)培養基中繁殖大腸桿菌S17-l^pir (λρίι·，recA，thi， pro, hsdR (rk-，mk+)，RP4-2, (TcR::Mu)，（KmR::Tn7)，[TmpR], 136852.doc •60- 1334884 [SmR])。在必需使用抗生素時，使用ι〇〇微克/毫升氨苄青黴素（Sigma)、50微克 /毫升萘啶酮酸（Ν5())(3ί§π^^σ 5〇(K5〇) 或100(K °°)微克/毫升康黴素（§igma)。藉著使每個大腸桿菌S17-l:λpir/pTEFl:[NK]35純種系和 AP225品系生長至後對數期，引起交配。將每種附加標籤-pTEF-Ι純種系的一等份5〇微升之培養物與ι5〇微升的 APP225培養物混合’然後將每種交配混合物各5〇微升點在先刖放在含有100 μΜ IPTG和10 mM MgS04之BHIV10培養盤上的0.22 μΜ濾紙上《在37°C和5%C〇2下培養過夜之後’從每個濾紙上將交配混合物洗到2毫升PBs中，並各取 200微升放在ΒΗΐνι«ΝπΚΐ(Η)培養盤上。在選擇性過夜生長之後’藉著以牙籤移至200微升BHIV〗0N50K50中，將菌落聚集在微量滴定盤内’確定在微量滴定盤的每孔中總是含有帶有相同序列標籤的轉因子突變種。在過夜生長之後，在母孔中加入50微升75°/。甘油’並將培養盤冷凍儲存在_8〇 °C下。 APP似乎不像敗血性巴斯德桿菌這麼偏愛迷你_Τη丨〇單元的多個插入。判定僅大約3%的突變種含有多個插入，其與先前報告的4%相符[Tascon等人，J Bacteriol. 175:5717-22 (1993)]。在ΛΡΡ中的問題，包括確認許多突變種（下文討論）含有23S RNA區域内的插入：總共28個突變種插入13個獨特的位置。這可能代表23S RNA含有優先的插入位置’且受到這些插入影響的APP生長，足以在宿主中導致不同的存活。使用APP 23S RNA探針的南方墨點 ^ 36852.doc IS] -61 - 1334884 刀析，暗不與在流行性感冒嗜血桿菌中的5個等人，Science 269:496·512 (1995)]，以及在大腸桿菌中7 個完整的操縱子[Blattner 等人，Science 277:1453 1474 (1997)]相比較，APP可能僅含有3個核糖體操縱子。該位置優先權及其對生長速率的影響，可能是"飽和突變生成作用的重要障礙，因為重大數目的純種系將在這些内含有插入，而對於獲得額外的獨特減毒突變，大量體積的篩選將是必要的。

實例7 對胸膜肺炎放線桿菌突變種進行豬的篩選使用豬氣管内注射的模式，篩選2〇個胸膜肺炎放線桿菌轉因子大變種的集合，其含有總共大約8〇〇個突變種。從-80°C的儲存中移出集合的胸膜肺炎放線桿菌轉因子大變種的冷凍培養盤，並藉著從各孔中移出2〇微升，至含有180微升BHIVi^^K5〇之新的96孔圓底培養盤（c〇rning

Costai·’ Cambridge，MA，USA)中，繼代培養之。在 37<>(：和 5% C〇2中培養該培養盤過夜，不需振盪。然後藉著從各孔中移出10微升，至每孔含有100微升BHIVio之新的平底％孔培養盤（Corning Costar)中，並在37它下培養，以15〇 rpm振盪，繼代培養該過夜培養盤。使用微量滴定盤讀取器監視OD562。在大約0.2至〇 25的〇〇562時，藉著混合從微量滴定盤每孔中各別取出的100微升，集合各盤形成”輸入集合”。以BHI適當地稀釋培養物至大約2X106CFU/毫升。藉著氣管内投藥，使用氣管内管，每種經過稀釋的集合各 -62- 136852.doc I S1 1334884 取4.0毫升來感染ι〇_2〇公斤的sPF豬（whiteshire Hamr〇c， Albion, IN)。在感染後大約20小時時，將所有存活的動物安樂死，並移出肺臟。藉著將15〇毫升無菌的pBS注入肺中，然後搮搓使液體分散，進行灌洗以便移出存活的細菌。回收灌洗液，並重複該過程兩次。以45〇 χ §離心灌洗液1〇分鐘，分離大的碎屑。然後以2,800 χ g離心上清液，使細菌形成小球。以1 〇-2至丨0-5之稀釋範圍，在bhiv1〇n5〇k5〇培養盤上，將小球再懸浮於5毫升b hi中。在過夜生長之後’在10毫升BHI肉湯中集合至少ι〇〇〇〇〇個菌落，形成回收集合"。藉著CTAB方法[Wilson,在Ausubel等人（編輯），Current Protocols in Molecular Biology，第 1冊 j〇hn Wiley and Sons，New York,第 2.4.1-2.4.5 頁（1997)中]，使用每種回收集合各0.7毫升的部份，來製備基因組dna。從動物中回收，從肺臟灌洗液中例行地是在丨〇8CFU的範圍内》

藉著按照先前描述的目視檢查和藉著半-定量分析，進行墨點沾染和評估兩者。所有的雜交和檢測均按照上述進行。簡言之，藉著初級PCR擴大作用製備探針，接著是想要產物的瓊脂糖凝膠純化，以及併入dig-dUTP的二級pcR 擴大作用。藉著Genosys Biotechnologies (The Woodlands, TX)合成寡核苷酸，包括 TEF5、TEF6、TEF24、TEF25、 TEF48和TEF62。亦使用引子TEF69、TEF65和TEF66倒轉 PCR反應並定序之。 TEF69 GACGTTTCCCGTTGAATATGGCTC 序列識別：166號 -63- 136852.doc ί S1 1334884 TEF65 GCCGGATCCGGGATCATATGACAAGA 序列識別：167號； TEF66 GACAAGATGTGTATCCACCTTAAC 序列識別：I68號然後以Hindlll消化已標示的PCR產物，從獨特的標籤區中分離不變的引子臂。切除含有已標示之可變標藏的區域，然後將整個凝膠片溶解於DIG EasyHyb中，並使其變性。製備墨點，並使用標準CSPD檢測草案檢測之。使軟片曝光以便進行目視評估，並對每個墨點試樣判定每秒的發光計數（LCPS)。使用每個突變種的LCPS^/LCPS回收比例來判定可能減毒的突變種。選擇按照在輸入集合中出現，但在回收集合中極度降低而選出的純種系，進行進一步的研究。在目視評估由墨點製成的軟片之後，亦選擇具有可疑之輸入/回收比例的額外純種系。總共選出110個純種系》實例8 確認胸膜肺炎放線桿義毒力基因藉著逆PCR和直接的產物定序，對110個突變種分別定出一部份位在側面的序列。按照上述，使用逆PCR，產生側面DNA產物，以便進行直接定序。使用得自PE Applied Biosystems (Foster City，CA)的BigDyeTM_ 料中止劑化學 (Dye Terminator Chemistry)套組進行定序反應，並在ABI Prism 377 DNA定序器上執行。使用定序者（Sequencer) 3.0 軟體（Genecodes, Corp., Ann Arbor, MI)集合並分析序列數據。使用 GCG程式[Devereux和 Haeberli, Wisconsin套裝版本 9.0，9.0 版 Genetics Computer Group, Inc., Madison 136852.doc -64- (1"7)]，在目前可獲得的資料庫中搜尋同種的序列。表2顯示確認並延伸的胸膜肺炎放線桿菌基因，使其開砍碥閱架構是可判定的。提供核铝酸序列的序列識別號从及所在之處推讨的胺基酸序列。表2 胸膜肺炎放線桿菌開放編閱架構 ,開放編閱架構 134號 132號 140號 152號 150號 160號 142號 146號 158號

aPtG exbB 〇mpj>5 〇mpP5~2 % fkPA rpmF

P〇tD yaeH apvC

pnp apvA-orl apvA-or2 apvB apvD 序列識別序列識別序列識別序列識別序列識別序列識別序列識別序列識別序列識別 ipdA密碍子·無中止密碼子序列識別：148號序列識別：156號序列識別：164號序列識別：128號無起始密碼子-中止密碼子 dksA 序列識別：136號 dnaK 序列識別：138號 HI0379 序列識別：144號無起始密碼子-無中止密碼子序列識別：154號序列識別：122號序列識別：124號序列識別：126號序列識別：130號 RNA或非編碼序列 tRNA-leu 序列識別：162號 tRNA-glu 序列識別：163號藉著與細菌資料庫相比較，分派在表3(下文，實例9)中列舉的假定身份。11〇個突變種代表Μ群獨特的轉因子插入。每個位點之不同突變的號碼，隨著一些總是在ORF中含有單一位置之插入的純種系，至在相同ORF之不同位置中含有插入的純種系而有所不同。篩選在110個突變種中檢測出的三種多個插入，藉著產生多個PCR譜帶，並產生多個序列電泳圖來判定。 136852.doc • 65- !334884 實例9 胸膜肺炎放線桿菌突變種與野外型App225的競爭性攻毒從原始集合盤中，從每個上文確認其在回收族群中缺乏或極度減少的獨特減毒突變種組中分離代表性的純種系，並用在與野外型品系（AP225)的競爭性攻毒實驗中，以便證實藉著轉因子突變作用引起的相對減毒作用。使突變種和野外型品系生長在BHIVl〇中，至〇.6_〇9的〇〇59〇。將野外型和突變種品系各大約5〇xl〇6CFU，加至4毫升·中。藉著氣管内投藥，利用氣管内管，使用總共4毫升之劑量來感染10-20公斤的卿豬。在感染後大約2〇小時時，安樂死所有存活的動物，並移出肺臟。按照上述進行肺臟灌洗。在㈣和麵vi〇n5〇k1〇0上進行培養盤計數，判定在輸入培養物和在肺臟灌洗試樣兩者之中，野外型對突變種的相對數目。按照[突變種CFU/野外型咖]“/[突變種CFU/野外型CFU]曰收，計算競爭指數⑹）。在3玲可能的轉因子突變種中，Μ個是明顯減毒的，且 $低於0.2的競爭指數（CI)。從—個集合中，選出以隨筛選結果為基礎，似乎未被減料轉因子突變該突變種在活體内具有大一。亦對該』 =7個=的競爭作用’結果CI為。.8。接著判定突種在2個本丙胺酸tRNAs之間含有插入。數在=中列舉獨特的減毒之單〜插入突變種的競爭指 =。、卿和exbB App突變種的競爭指數指出突變種不肖b元全有效地與野外型口糸 ”卜b系競爭，並因此是減毒的。 136852,doc -66 - 1334884

表3 胸膜肺炎放線桿菌突變種的毒力和建議功能突變種類似性假定或已知的功能 C.I. AP20A6 atpH ATP合成酶 .009 AP7F10 atpG ATP合成酶 .013 AP17C6 lpdA 二氫硫辛醯胺脫氫酶 .039 AP11E7 exbB 鐵化合物的運載 .003, .003, .006 AP3H7 potD 亞精胺/腐胺運載 .308 AP8H6 0mpP5 黏附素(adhesin)/OmpA同系物 .184 AP18H8 OmpP5-2 黏附素/OmpA同系物 .552 AP13E9 tig 肽基-丙基異構酶 .050 AP13C2 fkpA 肽基-丙基異構酶 <.001 AP15C11 pnp 多核苷酸碟酸化酶 .032 AP18F12 hupA 類組織蛋白-蛋白質 .001 AP20F8 dksA dnaK突變作用的劑量依賴性抑制劑 .075 AP5G4 dnaK 熱休克蛋白質-分子的監護蛋白 (chaperone) .376 AP17C9 tRNA-leu 蛋白質合成 .059 AP5D6 tRNA-glu 蛋白質合成 .055 AP18B2 rpmF 蛋白質合成 .112 AP10E7 yaeA 未知 .001 AP19A5 HI0379 未知 .061 AP10C10 apvA 未知 .157 AP18F5 apvB 未知 .103 AP2A6 apvC 未知 .091 AP2C11 apvD 未知 .014 CI的精確性似乎是極佳的，像是exbB突變種在3種不同的動物中競爭，產生0.003、0.003和0.006的CI。以在下文描述的實例11中，利用7個突變種對豬（n=8)之初步疫苗接種的結果為基礎，進一步證實以在大動物研究中的一次競 136852.doc -67- 1334884 爭為基礎，使用競爭指數的數目來分派減毒作用。實例1 〇定出減毒之胸膜肺炎放線桿菌毒力基因的特徵已確認的胸膜肺炎放線桿菌基因，代表4個廣泛功能的種類：生物合成的酵素、細胞的運載組份、細胞的調節組份和未知的。 atpG基因，編碼f〇f丨H+-ATP酶複合物的亞單元，可能在ATP的產製上，或在質子運載上藉著水解Ατρ產生功能。亦在敗血性巴斯德桿菌中確認了相關的atpQ減毒突變種*亦確認另一個atp基因，atpH，其編碼FiS亞單元。 atp突變種的表現型包括不能適應的感酸性表現型[F〇ster， J. Bacteriol. m:6896·6·⑽叫]，在鼠傷寒沙門氏桿菌[.士 del Portillo等人，infect· Immun 61:4489 4492 (1993)]和敗血性巴斯德桿® (在上文）中喪失毒力，且在兩者中降低轉化頻率，並在流行性桿冒嗜血桿菌Rd中誘導能力調節基因 [Gwinn 等人，J Bacteriol. 179:7315-20 (1997)]。

LpdA是二氫硫辛醢胺脫氫酶，其為兩種酵素複合物的組份：丙酮酸脫氫酶和2-明戊二酸脫氫酶。雖然與毒力的關係是未知的，但當暴露在得自人類的殺細菌蛋白質下時，在鼠傷寒沙門氏桿菌中誘導LpdA的產製，這可能暗示該誘導作用可能涉及企圖修補外膜[Qi等人，MW Microbiol. 17:523-31 (1995)] 〇生長和存活必要之珍貴化合物的運载，在活體内是很重要的。ExbB是TonB運載藉人你认 4|? /v 戰稷口物的一部份[Hantke和 136852.doc •68- 1334884

Zimmerman，Microbiology Letters. 49:3 1-35 (1981)]，以至少兩種不同的方式與TonB產生交互作用[Karlsson等人，

Mol Microbiol. 8:389-96 (1993)，Karlsson等人，Mol Microbiol. 8:379-88 (1993)]。獲得鐵對於病原而言是必要的。在該研究中，已經在APP和敗血性巴斯德桿菌兩者中確認減毒的 exbB突變種。在其他細菌中，已經確認數個T〇nB_依賴性鐵受體[Biswas等人，Mol. Microbiol. 24:169-179 (1997)， Braun, FEMS Microbiol Rev. 16:295-307 (1995)，Elkins等 • 人 ’ Infect. Immun. 66:151-160 (1998)，Occhino等人，Mol Microbiol· 29:1493-507 (1998)，St〇jiljk〇vic^Srinivasan，j Bactedol. 179:805-12 (1997)]。為了獲得鐵，胸膜肺炎放線桿菌產生2個鐵傳遞蛋白-結合蛋白質，其同樣地依賴 ExbB/ExbD/TonB系統。P〇tD是胞漿周圍的結合蛋白質，其為亞精胺（多胺）運载所必需的[Kashivvagi等人，j Bi〇1 Chem. 268:19358-63 (1993)]。巴斯德桿菌科的其他成員， φ 溶血性巴斯德桿菌，含有P〇tD的同系物（Lpp38)，其在恢復期中為主要的免疫原，或是免疫牛的外膜蛋白質 [Pandher和 Murphy, Vet Microbiol. 51:331-41 (1996)]。在溶血性巴斯德桿菌中，PotD似乎與内和外膜兩者有關。 P〇tD在毒力上，或在與保護性抗體之交互作用上的角色是未知的，雖然先前的研究已經顯示肺炎鏈球菌 (StreptOCOCCUS pneum〇niae)之p〇tD突變種是減毒的等人，Infect. Immun. 66:5620-9 (1998)]。亦確認相對上較少的"典型毒力因子"，像是黏附素或毒 136852.doc •69· 1334884 素’除了流行性感冒嗜血桿菌OMP P5的同系物之外。流行性感冒嗜血桿菌OMP P5是主要的外膜蛋白質，其與蛋白質之OmpA波林（porin)家族有關[Munson等人，M Infect Immiin. 61:4017-20 (1993)]。已經顯示在不可作為代表之流行性感冒嗜血桿菌中的OMP P5，編碼以絲狀結構表現之細毛（fimbrial)亞單元蛋白質[Sirak〇va等人，

Immun. 62:2002-2〇(1994)]，其貢獻毒力，並與黏液素和表皮細胞兩者結合[Miyamoto 和 Bakaletz，Microb Path〇g. 21:343-56 (1996)，Reddy等人，lnfect. immun. 64:1477-9 (1996)，Sirakova等人，Infect. Immun. 62:2002-20 (1994)]。一項重大的發現是確認兩個不同的〇rF4，其似乎編碼〇Mp P5同系物。亦有具有兩個極類似蛋白質的案例，得自杜克雷氏嗜jk桿菌的MOMP和Omp A2。尚待決定這兩者在功能上是否涉及細毛的產製，且兩個這類〇RFs的出現是否代表分歧的複製，具有多餘或互補的功能。有趣的是，兩個 OMP P5突變種似乎具有不同的（^值，暗示在對於單一副本之必要性或功能性上的差異。已經顯示OMP P5在慢性感染期間’經歷分子改變[Duim等人，Infect. Immun. 65:1351-1356 (1997)]，然而，這似乎限於單一基因經歷點突變，產生胺基酸改變，而非歸因於多個基因之差示表現的"型式轉變··。

蛋白質折疊酵素對於胞漿周圍和細胞外蛋白質的有效折疊而言’是很重要的輔助劑，並確認出其產物具有肽基_ 丙基異構酶活性的兩個基因：fkpA和tig(觸發因子）。FkpA 136852.doc

ί SI -70- 1334884 是胞漿周圍蛋白質，其為FK506-結合蛋白質家族的成員 [Horne和 Young, Arch Microbiol. 163:357-65 (1995); Missiakas 等人，Mol Microbiol. 21:871-84 (1996)]。已經顯示 FkpA 助成鼠傷寒沙門氏桿菌的細胞内存活[Horne等人，Infect. Immun. 65:806-10 (1997)，而嗜肺軍團桿菌（Legionella Pneumophila)同系物，mip [Engleberg等人，Infect· Immun. 57:1263-1270 (1989)]，則負貴毒力和感染巨噬細胞 [Cianciotto 等人，J. Infect· Dis. 162:121-6 (1990);. Cianciotto等人，Infect. Immun. 57:1255-1262 (1989)]。Tig或觸發因子 [Crooke和 Wickner，Proc. Natl. Acad. Sci. USA. 84:5216-20 (1987)， Guthrie 和 Wickner，J Bacteriol. 172:5555-62 (1990)，在 Hesterkamp和 Bukau，FEBS Lett. 389:32-4 (1996)中回顧]，是含有代表性之FKBP區域的肽基丙基異構酶[Callebaut和 Mornon，FEBS Lett. 374:211-215 (1995)]，但不受 FK506 影響[Stoller等人，EMBO J. 14:4939-48(1995)]。已經顯示 tig 與核糖體和初生多肽鏈有關[Hesterkamp等人，Proc Natl Acad Sci USA 93:4437-41 (1996)，Stoller等人，EMBO J. 14:4939-48 (1995)]。可能的角色包括在大腸桿菌中，對細胞分裂有未知的影響[Guthrie和Wickner，J Bacteriol. 172:5555-62 (1990)]，在酿腹鍵球菌（Streptococcus pyogenes)半胱胺酸蛋白酶的分泌和激活作用中的角色 [Lyon等人，EMBO J. 17:6263-75 (1998)]，以及在枯草桿菌（Bacillus subtilis)中，在饑餓條件下存活[Gothel等人， Biochemistry 37:13392-9 (1998)]。 71 I36852.doc i S1 1334884 細菌病原使用許多機制，對等地調節基因表現，以便在宿主内各式各樣的環境中存活。在原核生物中，在mRNA 穩定性上的差異可調節基因表現[Be丨“⑶和Higgins，Gene 72:15-23 (1988)]。例如，rnr(vacB)是在副痢疾桿菌 (Shigella flexneri)中，表現質體攜帶之毒力基因所必需的

[Tobe等人，J Bacteriol. 174:6359-67 (1992)]，並編碼RNA 酶 R核糖核酸酶[ChengfA，j.Bi〇i.chem.273:14077-14080 (1998)]。PNP是多核苷酸磷酸化酶，其涉及mRNA

的降解。Null pnp/rnr突變種是致命的，暗示可能有功能的重疊。因此很可能rnr和pnp兩者均涉及毒力基因表現的調節。在老鼠敗血症模式中（實例2) ’敗血性巴斯德桿菌的 pnp突變種是無毒力的。在枯草桿菌中，其他與pnp有關之表現型包括能力上的缺陷和感冷性[Wang和Bechhofer，J

Bacteriol. 178:2375-82 (1996)]。

Hup A是細菌類-組織蛋白蛋白質，其在大腸桿菌中與 HupB組合’構成HU蛋白質。報告已暗示hupA和hupB單一的突變種’未證明任何可觀察到的表現型[Huisman等人， J Bacteriol. 171:3704-12 (1989)，Wada等人，J Mol Biol. 204:581-91 (1988)]，然而，已經顯示hupA-hupB雙重突變種是感冷性的’感熱休克性的，並在許多形式的指定位置之DNA重組作用中被阻斷[wada等人，J Mol Biol. 204:581-91 (1988)，Wada等人，Gene. 76:345-52(1989)]。

先前一項受到制限的資料指出，hupA直接涉及毒力 [Turner等人，Infect. Immun. 66:2099-106 (1998)] ° hupA -72- 136852.doc i Si 減毒的機制仍是未知的。

DnaK是已熟知和高度保留的熱休克蛋白質，涉及對各種緊迫丨生環境改變的調節反應[在LincjqUist和Craig，Annu

Rev Genet· 22:631-77 (1988)中回顧]。DnaK在小腸結腸型耶爾森氏菌中’在被巨噬細胞吞噬之後，亦是最顯著誘發的緊 k 蛋白質之一 [Yamam〇t〇

38.295 300 (1994)]’ 而豬布氏桿菌（Bruceiia su is)的 dnaK 大變種’無法在人類類-巨噬細胞的細胞中繁殖[K〇Mer等人 ’ Mol Microbiol. 20:701-12 (1996)] » 相反的，其他的細胞内病原卓核白血球增多性李士德菌（Listeria monocytogenes)，在吞嗤作用之後未顯示dnaK的誘導[Hanawa等人，Infect

Immun. 63:4595-9 (1995)]。霍亂弧菌（Vibrio cholera)的 dnaK突變種，在活體外影響ToxR及其調節毒力因子的產製’但並未從在活體内生長的細胞中獲得類似的結果 [Chakrabati等人，infect· immun 67:1025-1033 (1999)]。胸臈肺炎放線桿菌dnaK突變種的CI，比大多數減毒的突變種更高，雖然仍約為陽性對照組品系的一半。

DksA在大腸桿菌之dnaK突變種中，是絲狀和感溫性生長的劑量依賴性抑制劑[Kang和Craig，J Bacteriol. 172:2055-64 (1990)]。目前還未對DksA定義分子功能，但在雞和剛畔化的雛雞中’已經確認該基因對於鼠傷寒沙門氏桿菌的毒力是很重要的[Turner等人，infect. Immun 66:2099-106 (1998)]。在那項工作中，注意到北“突變種不能利用葡萄糖或組胺酸良好地生長，但可利用縠胺醢胺 136852.doc -73- 1334884 或榖胺酸作為唯一碳來源，良好地生長。該觀察可能表示 dksA突變種是在穀胺酸之生物合成中，以某種方式受到傷害的[Turner等人，Infect. Immun. 66:2099-106 (1998)]。確認三個在蛋白質合成中扮演某種角色的基因：tRNA-leu、tRNA-glu和rpmF。除了蛋白質合成之外，tRNA's在肽聚糖的合成[Stewart等人，Nature 230:36-38 (1971)]、卟吩環合成[Jahn等人，Trends Biochem Sci. 17:215-8 (1992)]、為了降解而猫準蛋白質[Tobias等人，Science 254:1374-7 (1991)]、轉譯後在蛋白質中添加胺基酸[Leibowitz和 Soffer，B.B.R.C. 36:47-53 (1969)]，以及細菌-真核生物之交互作用的調解[Gray等人，J Bacteriol. 174:1086-98 (1992)， Hromockyj等人，Mol Microbiol.6:21 13-24 (1992)]中，亦具有各式各樣功能性的角色。更特定而言，tRNA-leu涉及轉錄減毒作用[Carter 等人，Proc. Natl. Acad. Sci. USA 83:8127-8131 (1986)]、由山梅花假單孢菌（Pseudomonas syringae)形成的病變[Rich和 Willis, J Bacteriol. 179:2247-58 (1997)]，以及泌尿道病原性之大腸桿菌的毒力 [Dobrindt 等人，FEMS Microbiol Lett. 162:135-141 (1998), Ritter等人，Mol Microbiol. 17:109-21 (1995)]。不知道我們確認的tRNA是否代表胸膜肺炎放線桿菌中，tRNA-leu的次要物種。無論如何，tRNA-leu可能具有各種功能中的任何一種。RpmF是核糖體蛋白質，在大腸桿菌中，其基因亦是含有脂肪酸生物合成酵素之操縱子的一部份。將需要進一步的研究，指出在胸膜肺炎放線桿菌中是否也是這樣， 136852.doc -74- 1334884

雖然在流行性感冒嗜血桿菌中出現相同的fab基因和rpmF 聚集[Fleischmann等人，Science 269:496-512 (1995)]。fab 基因的表現並非必要的，視起源於rpmF上游的轉錄本而定’因為已經在rpmF中確認有第二個啟動基因[zhang和 Cronan, Jr., J Bacteriol. 180:3295-303 (1998)] 〇最後一種減毒突變種，包括在未知功能的基因，或在先刖尚未確認之基因中的突變作用。先前已經分別在大腸桿菌[Blattner等人，Science 277:1453-1474 (1997)]和流行性感冒嗜血桿菌[Fleischmann 等人，Science 269:496-512 (1995)]中確認TyaeA和HI03 79的同系物。已經將其餘未知的命名為胸膜肺炎放線桿菌毒力基因（apV)。apVC基因對 ΗΙ0893顯示出明顯的類似性，然而，所提出之hi〇893的類似性是不確定的，就像轉錄阻遏物類似脂肪酸反應調節者 Bm3Rl — 樣[Palmer 等人，J Biol Chem. 273:18109-16 (1998)]。apvD基因亦是最類似得自大腸桿菌的假定膜蛋白 (b0878)，其具有未知的功能[Blattner等人，Science 277:1453-1474 (1997)]。另外兩個未知物，apVA 和 apvB，在公告之資料庫中無顯著相配的。實例11 胸膜肺炎放線桿菌突變種的安全性和效力使用9組（n=8) SPF豬（4-5週齡，3-10公斤）來判定7個胸膜肺炎放線桿菌突變種，作為活的減毒疫苗品系的安全性和效力。在第1天，以每種突變種各101GCFU，經鼻内感染7 組。一組在第1和15天時，接種市售的疫苗Pleuromune -75- 136852.doc i S] 1334884 (Bayer)，而一個無經驗組則不接種疫苗。在第29天時，以每隻豬1-5x105CFU的野外型APP225，經鼻内攻毒所有的組別。在研究的第42天時，安樂死所有存活的動物，並進行屍體解剖。在表4中出示結果。表4 胸膜肺炎放線桿菌突變種的效力在鼻内攻毒之後的死亡率％疫苗疫苗接種攻毒 Pleuromune 0 37.5 exbB 0 0 tig 12.5 0 fkp A 12.5 0 HI0385 50.0 0 pnp 0 0 yaeE 0 0 atpG 0 0 無 N/A 50.0 當以鼻内1 010CFU之劑量投與時，exbB、atpG、pnp和 yaeA突變種並未引起死亡。fkpA和tig突變種組各有1隻死亡，而HI0379組（在實例9中顯示為受試的7個突變種中最高的CI)有4隻死亡。使用該模式時野外型LD5Q通常是lxl 07 CFU，表示這些突變種均為至少100倍減毒的，且在CI和減毒之間有合理的關連。實例12 確認溶血性巴斯德桿菌之物種同系物以在敗血性巴斯德桿菌和胸膜肺炎放線桿菌中確認的毒力基因之序列為基礎，企圖在溶金性巴斯德桿菌中進行相關基因的確認，也就是物種同系物。利用PCR，以下文出 136852.doc •76· 1334884 示之簡併引子，企圖按照指示擴大溶血性巴斯德桿菌基因。由 Sigma-Genosys (The Woodlands, TX)合成的引子序列，包括標準單一字母名稱，其中B代表（C、G或T)，D代表（G、A或T)，Η代表（A、C或T)，K代表（G或T)，Μ代表 (Α或C)，Ν代表（A、G、C或Τ)，R代表（Α或G)，S代表或C)，V代表（G、Α或C)，W代表（Α或Τ)，且γ代表（C或 T)。 atp GTEF146 ATG GCN GGN GCN AAR GAR AT 序列識別 176號 TEF148 GCN GCY TTC ATN GCN ACC AT 序列識別 177號 gua BTEF240 GGN TTY ATY CAY AAA AAY ATG 序列識別 178號 TEF243 TCT TTN GTR ATN GTN ACA TCR TG 序列識別 179號 pnp TEF141 GCS GGY AAA CCR CGT TGG GAT TGG 序列識別 180號 TEF142 CRC CTA ARA TRT CTG AAA GCA CCA C 序列識別 181 號

pur FTEF244ATGTGYGGNATYGTNGGNAT TEF247 CAT ATC AAT ACC ATA CAC ATT 序列識別182號序列識別183號序列識別184號序列識別185號

yjg FTEF162 GGN CCN ΤΑΥ GTN CAR G TEF 163 NGC NAC YTC NAC RCA 為了擴大最初簡併的PCR產物，使用3.3X XL緩衝溶液II (PE Applied Biosystems)、200 μΜ dNTPs、適當的引子各 25微微莫耳、0.8mM MgCl2、0.5單位rTth DNA聚合酶、 XL (PE Applied Biosystems)和大約 1微克 TF1 DNA，建立 -77- I36852.doc IS! 1334884 50微升反應。循環條件為94°C 1.5分鐘；接著是94°C 15秒，40-60°(： 6〇秒，72°C 1.5分鐘的35次循環；以及最後保持在72°C下5分鐘。使用QIAGEN凝膠萃取套組（QIAGEN，Valencia CA)，從瓊脂糖凝膠中純化每個PCR產物的譜帶。使用得自 PE Applied Biosystems (Foster City，CA)的料中止劑化學（Dye Terminator Chemistry)套組進行定序反應，並在ABI Prism 377 DNA定序器上執行。獲得每個純種系開放編閱架構（ORF)的雙股序列。使用定序者 3.0軟體（Genecodes，Corp.，Ann Arbor, MI)集合並分析序列數據。使用GCG程式，藉著在目前可獲得的資料庫中搜尋同種的序列，證實ORF的身份。使用 Vectorette 套組（Genosys Biotechnologies, The Woodlands, TX)，獲得每個基因額外的側面序列。根據製造者建議的草案，製備Vectorette庫。使用Perkin Elmer Applied Biosystems GeneAmp XL PCR套組組份，利用下列的反應條件，產生Vectorette PCR產物。使用3.3X XL緩衝溶液II (PE Applied Biosystems)、200 μΜ dNTPs ' 適當的引子各25微微莫耳（下示）、0.8 mM MgCl2、0.5單位rTth DNA聚合酶、XL(PE Applied Biosystems)和 1微升適當的 vectorette庫建立50微升反應。循環條件為94°C 1.5分鐘；接著是94°C20秒，6〇t45秒，72°C4分鐘的35次循環；以及最後保持在72°C下7分鐘。每個庫的第二個引子為製造者的vectorette引子。 •78- 136852.doc IS1 1334884 表5 基因 Vectorette 庫引子(們） atpG Bglll, Hindlll TEF217 GAAGCCGCCATACGCTCTTGGG 序列舖.別186號 Clal TEF218 GTTGCTTCCTTTGCCTGCACTGG 序列識別187號 guaB EcoRI TEF265 GGCTCAGAAACAATACCACTTTCA 序列識.別188號 Hindlll, TaqI TEF268 GCACCAAAGCAGAATTTGTCC 序列識別189號 pnp Clal, Hindi TEF219 GGTGATGATGTCGATGATAGTCCC 序列識別190號 TaqI TEF220 GGCGTATTAGCCGTGATGCCAACC 序列镞別191號 BamHI TEF286 GACCACTTAGGCGATATGGACTT 序列識別192號 purF TaqI TEF271 ACCATCATAAATCGCCTGATTC 序列識別193號 TEF292 ACCTGCGGCATCTTGTCCTC 序列識別194鱿 Hindi TEF274 ACGGGTTTATTTTGCCTCTG 序列識別195號 yjgp Clal TEF221 CGCCGGTTTCAGGATTCACGGG 序列謐別196號 EcorV TEF281 CTGAACAACGTGAAAGCCAT 序列識別197號

按照上述以譜帶純化Vectorette PCR產物，並定序。分別在序列識別166、168、170、172和174號中陳述atpG、 guaB、pnp、purF和yjgF基因的多核嘗酸序列。分別在序列識別167、169、171、173和175號中陳述由這些基因編碼的多肤。對熟諳此藝者而言，預期在本發明中發生許多修改和變化，如同在上文解釋性之實例中陳述的》最後，應該只將在附錄之申請專利範圍中發表的這類限制，放置在本發明中。 -79- 136852.doc

I 1334884 序列表 <110> Lowery E.# David, et al. <120>抗-扣茵疫苗组合物 <130> 28341/00435 <140> 09/809,665 <141> 2001-03-15 <150> 60/153,453 <151> 1.999-09-10 <150> 60/128,689 <151> 1999-04-09 <150> 09/545,199 <151> 2000-04-06

<160> 197 <170> Patentln Ver. 2.0

<210> 1 <211> 1112 <212> DNA <2 η3 >敗血性巴斯德桿菌 <220>

<221> CDS <222> (210) . . (1001) <220> <223> atpB <220>

< 2 21 > jnisc 特徵 <222> 1099 <223> n=A或T或G或C

<220> <221> misc特徵 Π04" <223> n=A或T或G或C <400> 1 gtcaacaaca ttttatggtg gagaggccgt taaatttata tccacaattt ttttgattgt acttgctttt aaactgttca attcaatgca ttttattgca ttttttgttg gatattttat 120 aacaatagtt ttaaacaata ttcttccatt ttttataagt aagtacttaa atataaagca 180 ttttcataaa tatcaataaa ggattagtt atg gca gca gag ctt aca aca gcg 233 Met 1 Ala Ala Glu Leu 5 Thr Thr Ala 99a Cat att ggg cac cat tta gca ttc ttg aaa aca ggg gat tct ttc 281 Gly Tyr lie Gly His His Leu Ala Phe Leu Lys Thr Gly Asp Ser Phe 10 15 20 136852-序列表,doc 1334884 tgg cat gtt cat tta gat acc ctt eta ttt tea att att tea ggt gca 329

Trp His Val His Leu Asp Thr Leu Leu Phe Ser lie lie Ser Gly Ala 25 30 35 40 att ttt ctt ttt gtt ttt tea aaa gtt gca aaa aaa gca aeg ccg ggt 377

He Phe Leu Phe Val Phe Ser Lys Val Ala Lys Lys Ala Thr Pro Gly 45 50 55 gtg cct age aag atg caa tgt ttt gtt gag ata atg gtt gat tgg att 425

Val Pro Ser Lys Met Gin Cys Phe Val Glu He Met Val Asp Trp lie 60 65 70 gat ggg ate gta aaa gaa aat ttc cat ggt cct cgt cat get gtt gga 473

Asp Gly lie Val Lys Glu Asn Phe His Gly Pro Arg His Ala Val Gly 75 80 85 cca tta gca tta act att ttc tgc tgg gta ttc att atg aat get ate 521

Pro Leu Ala Leu Thr lie Phe Cys Trp Val Phe lie Met Asn Ala lie 90 95 100

gat ttg ate cca gta gat ttc eta cct caa tta gcc cat tta ttt ggt 569

Asp Leu lie Pro Val Asp Phe Leu Pro Gin Leu Ala His Leu Phe Gly 105 110 115 120 att gaa tac tta aga get gtt cca aca gca gat ate agt gga aca tta 617 lie Glu Tyr Leu Arg Ala Val Pro Thr Ala Asp lie Ser Gly Thr Leu 125 130 135 ggc tta tea att ggt gtc ttc ttc tta att att tcc tat aca ate aaa 665

Gly Leu Ser lie Gly Val Phe Phe Leu lie lie Phe Tyr Thr lie Lys 140 145 150 tea aaa ggt atg agt ggc ctt gtt aaa gaa tat aeg ctt cat cct ttt 713

Ser Lys Gly Met Ser Gly Phe Val Lys Glu Tyr Thr Leu His Pro Phe 155 160 165 aat cat cct ttg tta att ccg gtt aac tta geg ctt gaa tea gtc aca 761

Asn His Pro Leu Leu lie Pro Val Asn Leu Ala Leu Glu Ser Val Thr 170 175 180 tta tta gca aaa cct gtt tet ttg geg ttc cgt ctt etc ggg aat atg 809

Leu Leu Ala Lys Pro Val Ser Leu Ala Phe Arg Leu Phe Gly Asn Met 185 190 195 200

tat gca ggt gaa ctt ate ttt att ctt att gca gtg atg tac atg gca 857

Tyr Ala Gly Glu Leu lie Phe lie Leu lie Ala Val Met Tyr Met Ala 205 210 215 aat aat ttt gca ctt aat tea atg ggt att ttc atg cat ttg get tgg 905

Asn Asn Phe Ala Leu Asn Ser Met Gly lie Phe Met His Leu Ala Trp 220 225 230 get att ttc cat att ctt gtg att acc tta caa gca ttt att ttt atg 953

Ala lie Phe His lie Leu Val lie Thr Leu Gin Ala Phe lie Phe Met 235 240 245 atg ctt aca gtg gtc tat ttg agt atg ggt tat aac aaa gca gaa cac 1001

Met Leu Thr Val Val Tyr Leu Ser Met: Gly Tyr Asn Lys Ala Glu His 250 255 260 taatttttta caaacaaaac caaaccttgg gtctaaattt caatcttatg gagaacatta 1061 tggaacaccg taattaccac aacaatcatc gcacccgnaa ttnttcttgc t 1112 -2- 136852·序列表.doc 1334884 <210> 2 <2XX> 264 <212:>蛋白質 <213>敗血性巴斯德样菌 <400> 2

Met Ala Ala Glu Leu Thr Thr Ala Gly Tyr lie Gly His His Leu Ala IS 10 15

Phe Leu Lys Thr Gly Asp Ser Phe Trp His Val His Leu Asp Thr Leu 20 25 30

Leu Phe Ser lie lie Ser Gly Ala lie Phe Leu Phe Val Phe Ser Lys 35 40 45

Val Ala Lys Lys Ala Thr Pro Gly Val Pro Ser Lys Met Gin Cys Phe 50 55 60

Val Glu lie Met Val Asp Trp lie Asp Gly lie Val Lys Glu Asn Phe 65 70 75 80

His Gly Pro Arg His Ala Val Gly Pro Leu Ala Leu Thr lie Phe Cys 85 90 95

Trp Val Phe He Met Asn Ala lie Asp Leu lie Pro Val Asp Phe Leu 100 105 110

Pro Gin Leu Ala His Leu Phe Gly He Glu Tyr Leu Arg Ala Val Pro 115 120 125

Thr Ala Asp lie Ser Gly Thr Leu Gly Leu Ser He Gly Val Phe Phe 130 135 140

Leu lie lie Phe Tyr Thr lie Lys Ser Lys Gly Met Ser Gly Phe Val * 145 150 155 160

Lys Glu Tyr Thr Leu His Pro Phe Asn His Pro Leu Leu lie Pro Val 165 170 175

Asn Leu Ala Leu Glu Ser Val Thr Leu Leu Ala Lys Pro Val Ser Leu 160 185 190

Ala Phe Arg Leu Phe Gly Asn Met Tyr Ala Gly Glu Leu lie Phe lie 195 200 205

Leu lie Ala Val Met Tyr Met Ala Asn Asn Phe Ala Leu Asn Ser Met 210 215 220

Gly lie Phe Met His Leu Ala Trp Ala lie Phe His He Leu Val lie 225 230 235 240

Thr Leu Gin Ala Phe lie Phe Met Met Leu Thr Val Val Tyr Leu Ser 245 250 255

Met Gly Tyr Asn Lys Ala Glu His 260

<210> 3 <211> 1972 <212> DNA <213>敗血性巴斯德样菌 136852-序列表.doc Ιΐΐ <220>

<221> CDS <222> (364)..(1230) <220> <223> atpG <400> 3 agcgggccat ttggctcagt tcggcttcgg attcttatga tgcacacgta agcaattatc 60 acatggtcaa aaagtaactg aattattgaa acaaaaccaa tactctccgt tatctgtagc 120 acaacaagca ttagtgttat ttgcagtaga gtttggttac ttagaagaag tggacttaga 180 tcgtattggt tcacttgaat cagcactttt agagtatgct aaccataact atgctgattt 240 tatgcgtgag ttaacccaat ctggcaatta caatgatgaa attaaagagt cattaaaagg 300 cattttggat agcttcaaag caaacagtgc gtggtaagtt aacactttaa atggagagac 360 aaa atg gca ggt get aaa gag ata aga acc aaa ate geg agt gta aaa 408

Met Ala Gly Ala Lys Glu lie Arg Thr Lys lie Ala Ser Val Lys 1 5 10 15 agt aca caa aaa att act aaa geg atg gaa atg gtt get gee teg aaa 456

Ser Thr Gin Lys lie Thr Lys Ala Met Glu Met Val Ala Ala Ser Lys 20 25 30 atg cgt aaa aeg caa gaa ege atg tet tet tea ege cct Cat tea gaa 504

Met Arg Lys Thr Gin Glu Arg Met Ser Ser Ser Arg Pro Tyr Ser Glu 35 40 45 aca ata cgt aac gtg att age cac gtt tee aaa gca aeg att ggt tac 552

Thr lie Arg Asn Val lie Ser His Val Ser Lys Ala Thr lie Gly Tyr 50 55 60 aag cat cca ttt tta gtg gat ege gaa gta aaa aaa gtg ggc atg att 600

Lys His Pro Phe Leu Val Asp Arg Glu Val Lys Lys Val Gly Met lie 65 70 75 gtt gtg tee aca gat cgt ggt ett tgt ggt ggc tta aac gtg aac ttg 648

Val Val Ser Thr Asp Arg Gly Leu Cys Gly Gly Leu Asn Val Asn Leu 80 65 90 95 ttt aaa act gta tta aat gaa atg aaa gaa tgg aaa gaa aaa gat gtt 696

Phe Lys Thr Val Leu Asn Glu Met Lys Glu Trp Lys Glu Lys Asp Val 100 105 110 tee gtt caa ttg agt tta ate ggt tet aaa tet ate aac ttt ttc caa 744

Ser Val Gin Leu Ser Leu He Gly Ser Lys Ser He Asn Phe Phe Gin 115 120 125 tet ttg gga att aaa att tta acc caa gat tea ggt att ggt gat act 792

Ser Leu Gly He Lys He Leu Thr Gin Asp Ser Gly He Gly Asp Thr 130 135 140 ccc tet gtt gag cag tta att ggt tea gtc aat tet atg att gat get 840

Pro Ser Val Glu Gin Leu lie Gly Ser Val Asn Ser Met He Asp Ala 145 150 155 tat aaa aaa ggg gaa gta gat gtt gtg tat tta gtt tat aac aaa ttt 888

Tyr Lys Lys Gly Glu Val Asp Val Val Tyr Leu Val Tyr Asn Lys Phe 160 165 170 175 136852-序列表.doc -4- 1S1 1334884 ate aac aeg atg teg caa aag cca gta ttg gaa aaa tta att cca tta 936

He Asn Thr Met Ser Gin Lys Pro Val Leu Glu Lys Leu lie Pro Leu 180 185 190 cca gaa tta gat: aat gat gaa tta ggc gaa aga aaa caa gtt tgg gat 964

Pro Glu Leu Asp Asn Asp Glu Leu Gly Glu Arg Lys Gin Val Trp Asp 195 200 205 tat att tac gaa cct gat geg aaa gta tta tta gat aat tta ttg gtt 1032

Tyr lie Tyr Glu Pro Asp Ala Lys Val Leu Leu Asp Asn Leu Leu Val 210 215 220 cgt tat tta gaa tet cag get tat caa gca gca gtt gaa aac ett get 1080

Arg Tyr Leu Glu Ser Gin Val Tyx Gin Ala Ala Val Glu Asn Leu Ala 225 230 235 tet gag caa gee get ega atg gtc gee atg aaa gca gca aca gat aac 1128

Ser Glu Gin Ala Ala Arg Met Val Ala Met Lys Ala Ala Thr Asp Asn 240 245 250 255

gca ggt aac tta att aat gag tta cag tta gtc tat aac aaa get cgt 1176

Ala Gly Asn Leu lie Asn Glu Leu Gin Leu Val Tyr Asn Lys Ala Arg 260 265 270 caa gca agt att aca aat gaa tta aat gaa att gtt gee ggt gca gca 1224

Gin Ala Ser lie Thr Asn Glu Leu Asn Glu lie Val Ala Gly Ala Ala 275 280 285 gca att taacaaatag aggatcggta atggcaactg gaaaaattgt acaaatcatc 1280 Ala lie ggtgcggtta ttgacgttga attcccacaa gatgcagtac caaaagtata tgatgeetta 1340 aatgttgaaa caggtttagt aettgaagtt caacaacaat taggtggtgg tgtagttcgc 1400 tgtatcgcaa tgggatcatc tgatggatta aaacgcggtt taagcgtaac aaatacgaat 1460 aacccaattt ctgttccagt gggaacgaaa acattgggtc gtatcatgaa cgtattgggt 1520 gaaccaatcg atgagcaagg tgaaatcggt gcagaagaga attggtctat tcaccgtgcg 1580 ccaccaagtt atgaagaaca atctaacagt actgaacttt tagaaacggg aattaaagtt 1640

ategaettag tttgtccgtt tgcgaaaggg ggtaaagtag gtttattcgg tggtgcgggt 1700 gtcggtaaaa ccgtcaatat gatggaatta atccgtaaca tcgcaattga gcactcaggt 1760 tactctgtct ttgcgggggt aggtgagcgt aegegtgaag gtaaegaett ctatcatgag 1820 atgaaagact etaaegtatt agataaagtg* tctcttgttt atggtcaaat gaacgagcca 1880 ccaggtaacc gtttacgtgt ggcattaaca ggcttaacta tggcggaaaa attccgtgat 1940 gaaggtcgtg atgtettatt ettegttgat aa 1972 <210> 4 <211> 289 <212>蛋白質 <213>敗血性巴斯德样菌 <4 00 > 4

Met Ala Gly Ala Lys Glu He Arg Thr Lys lie Ala Ser Val Lys Ser 136852-序列表.doc

l SI 1334884 1 5 10 15

Thr Gin Lys lie Thr Lys A上a Met Glu Met Vai Ala Aia Ser bys Met 20 25 30

Arg Lys Thr Gin Glu Arg Met Ser Ser Ser Arg Pro Tyr Ser Glu Thr 35 40 45 lie Arg Asn Val He Ser His Val Ser Lys Ala Thr lie Gly Tyr Lys 50 55 60

His Pro Phe Leu Val Asp Arg Glu Val Lys Lys Val GXy Met lie Val 65 70 75 80

Val Ser Thr Asp Arg Gly Leu Cys Gly Gly Leu Asn Val Asn Leu Phe 85 90 95

Lys Thr Val Leu Asn Glu Met Lys Glu Trp Lys Glu Lys Asp Val Ser 100 105 110

Val Gin Leu Ser Leu lie Gly Ser Ly曰 Ser lie Asn Phe Phe Gin Ser 115 120 125

Leu GXy He Lys lie Leu Thr Gin Asp Ser Gly He Gly Asp Thr Pro 130 135 140

Ser Val Glu Gin Leu lie Gly Ser Val Asn Ser Met lie Asp Ala Tyr 145 150 155 160

Lys Lys Gly Glu Val Asp Val Val Tyr Leu Val Tyr Asn Lys Phe lie 165 170 175

Asn Thr Met Ser Gin Lys Pro Val Leu Glu Lys Leu lie Pro Leu Pro 180 185 190

Glu Leu Asp Asn Asp Glu Leu Gly Glu Arg Lys Gin Val Trp Asp Tyr 195 200 205 lie Tyr Glu Pro Asp Ala Lys Val Leu Leu Asp Asn Leu Leu Val Arg 210 215 220

Tyr Leu Glu Ser Gin Val Tyr Gin Ala Ala Val Glu Asn Leu Ala Ser 225 230 235 240

Glu Gin Ala Ala Arg Met Val Ala Met Lys Ala Ala Thr Asp Asn Ala 245 250 255

Gly Asn Leu lie Asn Glu Leu Gin Leu Val Tyr Asn Lys Ala Arg Gin 260 265 270

Ala Ser lie Thr Asn Glu Leu Asn Glu lie Val Ala Gly Ala Ala Ala 275 280 285 lie

<210> 5 <211> 1357 <212> DNA <2 13 >敗血性巴斯德样菌 <220> -6- 136852-序列表.doc

I SI 1334884 <221> CDS <222> (1)..(813)

<220> <223> capSE

<400> 5 gtc gac tat att tat cat act gcc gca tta aag caa gtg cct tea tgc 48 Val Asp Tyr lie Tyr His Ala Ala Leu Lys Gin Val Pro Ser Cys 1 5 10 15 gag ttt tat ccg tta gag gca gtg aaa acc aat att tta ggt aeg gca 96 Glu Phe Tyr Pro Leu Glu Ala Val Lys Thr Asn lie Leu Gly Thr Ala 20 25 30 aat gtc tta gaa gcc gcc ate caa aac cag ata aaa ege gtc gtc tgt 144 Asn Val Leu Glu Ala Ala lie Gin Asn Gin lie Lys Arg Val Val Cys 35 40 45 ctt age aca gat aaa geg gtg tac cca att aat geg atg ggc att tet 1.92 Leu Ser Thr Asp Lys Ala Val Tyr Pro lie Asn Ala Met Gly lie Ser 50 55 60 aaa gca atg atg gaa aaa gtc ate ate gca aaa teg cgt aac eta gaa 240 Lys Ala Met Met Glu Lys Val lie lie Ala Lys Ser Arg Asn Leu Glu 65 70 75 80 ggc aca cca aeg aca ate tgt tgt act ege tat ggc aat gtc atg gca 288 Gly Thr Pro Thr Thr lie Cys Cys Thr Arg Tyr Gly Asn Val Met Ala 85 90 95 teg cgt ggt teg gtt ate cca tta ttt gtc gat caa ata cgt caa ggc 336 Ser Arg Gly Ser Val lie Pro Leu Phe Val Asp Gin lie Arg Gin Gly 100 105 110 aag cct ttt act att act gat cct gag atg aca ege ttt atg atg aca 384 Lys Pro Phe Thr lie Thr Asp Pro Glu Met Thr Arg Phe Met Met Thr 115 120 125 ttg gaa gat get gtg gat tta gtc eta tat gca ttt aaa aat ggt caa 432 Leu Glu Asp Ala Val Asp Leu Val Leu Tyr Ala Phe Lys Asn Gly Gin 130 135 140 aat ggt gat gtt ttt gta caa aaa gcc ecc gca gca acc att ggt acc 480 Asn Gly Asp Val Phe Val Gin Lys Ala Pro Ala Ala Thr lie Gly Thr 145 150 155 160 ctt gcc aaa gca att acc gaa tta tta tet gtc cca aat cac cct att 528 Leu Ala Lys Ala lie Thr Glu Leu Leu Ser Val Pro Asn His Pro lie 165 170 175 tcc act ata ggt aeg cgt cat gga gag aaa gca ttc gaa get tta tta 576 Ser lie He Gly Thr Arg His Gly Glu Lys Ala Phe Glu Ala Leu Leu 180 185 190 age cgt gaa gaa atg gtt cat gca att aat gaa ggt aat tat tat ege 624 Ser Arg Glu Glu Met Val His Ala lie Asn Glu Gly Asn Tyr Tyr Arg 195 200 205 ate cca gcc gat caa ege agt、 tta aat tac age aaa tat gtc gaa aaa 672 He Pro Ala Asp Gin Arg Ser Leu Asn Tyr Ser Lys Tyr Val Glu Lys 210 215 220 136852-序列表.doc IS1 1334884 ggg gaa cca aaa att acc gaa gtc acc gac tac aac tea cat aat act 720

Gly Glu Pro Lys lie Thr Glu Val Thr Asp Tyr Asn Ser His Asn Thr 225 2i0 235 240 gag cgt ttg act gtc aag gaa atg aag cag tea ctg ett aaa ett gaa 768

Glu Arg Leu Thr Val Lys Glu Met Lys Gin Leu Leu Leu Lys Leu Glu 245 250 255 ttc ata cag aaa atg att gag ggt gaa tac ate tea ccg gag gta 813

Phe lie Gin Lys Met lie Glu Gly Glu Tyr lie Ser Pro Glu Val 260 265 270 taaaaatgaa agtcttagta actggttcaa atggttttat tgcgaaaaat ctgattcagt 873 ctttatctga ggaacaagat attgagattt tatgttatca ccgtcaatcc tctgagaaaa 933 egettattea tcatgtattg agtgctgatt ggattattca tcttgcgggt gegaategte 993 cacctgaaga acaagaattt atgacatcaa atacacaatt gacggaaaaa atttgccgta 1053

ttttacagcg tcatcagaaa aaaacgcctt tgttatattc ctctagcatt caagtagaaa 1113 gtcccaaaat aagtaettat tcgcaaacca aattagaaag tgaatatcat gttcatcaat 1173 tacataaaga aaatggtaat ccgatttata tctgccgttt agctaatgtc tttggcaadt 1233 ggtcacgacc tcactataac teggtagteg ccacattttg ccataactta attcatgatt 1293 tacccatcga aattcatgat catactgcag aaataagget catttatatt gatgatgteg 1353 ttga 1357 <210> 6 <211> 271 <212>蛋白質 <213>敗血性巴斯德桿05 <400> 6

Val Asp Tyr lie Tyr His Ala Ala Ala Leu Lys Gin Val Pro Ser Cys 15 10 15

Glu Phe Tyr Pro Leu Glu Ala Val Lys Thr Asn lie Leu Gly Thr Ala 20 25 30

Asn Val Leu Glu Ala Ala lie Gin Asn Gin lie Lys Arg Val Val Cys 35 40 45

Leu Ser Thr Asp Lys Ala Val Tyr Pro lie Asn Ala Met Gly lie Ser 50 55 60

Lys Ala Met Met Glu Lys Val lie lie Ala Lys Ser Arg Asn Leu Glu 65 70 75 80

Gly Thr Pro Thr Thr lie Cys Cys Thr Arg Tyr Gly Asn Val Met Ala 85 90 95

Ser Arg Gly Ser Val lie Pro Leu Phe Val Asp Gin lie Arg Gin Gly 100 105 110

Lys Pro Phe Thr lie Thr Asp Pro Glu Met Thr Arg Phe Met Met Thr 115 120 125 136852·序列表.doc

1 SI 1334884

Leu Giu Asp Ala Val Asp Leu Val Leu Tyr Ala Phe Lys Asn Gly Gin 130 135 140

Asn Gly Asp Val Phe Val Gin hys Ala Pro Ala Ala Thr lie Gly Thr 145 ISO 155 160

Leu Ala Lys Ala lie Thr Glu Leu Leu Ser Val Pro Asn His Pro lie 165 170 175

Sar lie lie Gly Thr Arg His Gly Glu Lys Ala Phe Glu Ala Leu Leu 180 185 190

Ser Arg Glu Glu Met Val His Ala lie Asn Glu Gly Asn Tyr iyr Arg 195 200 205 lie Pro Ala Asp Gin Arg Ser Leu Asn Tyr Ser Lys Tyr Val Glu Lys 210 215 220

Gly Glu Pro Lys lie Thr Glu Val Thr Asp Tyr Asn Ser His Asn Thr 225 230 235 240

Glu Arg Leu Thr Val Lys Glu Met Lys Gin Leu Leu Leu Lys Leu Glu 245 250 255

Phe lie Gin Lys Met lie Glu Gly Glu Tyr lie Ser Pro Glu. Val 260 265 270 <210> 7 <211> 6132

<212> DNA <213>敗血性巴斯德桿菌 <220>

<221> CDS <222> (4032) .. (4727) <220> <223> devB <400> 7 gtcaacaatt accgcacttt agtggagtaa ataacaatgg cgaaaaagaa taacgggcat 60

gaaaaagagg atgatgttcg cctagataaa tggctttggg ctgcccgttt ttataaaaca 120 cgtactttag caaaagacat gattgatggc ggtaaagtgc attataatgg gcagcgcacg 180 aaacccaata aaacggttga aattggtggt gtgatcaaac ttcgtcaagg taatgacgaa 240 aaagaagtgg aagtgcttgc gctttctacg caacgtcgtg gggcgccaga agcacaattg 300 ttgtatcaag aaacagaaaa aagccttgaa caacgtgcga aaatggcgat tgcacgtaag 360 attaatgcat taacgatgcc gcatcctgat cgtcgcccga ataaaaaaga gcggcgtgat 420 ttattgaaat ttaaacatca agatagcttt tcatcttgat gatgtgattt acctactttt 4β0 cttattaaag aaaggaatat ggggaagctg tgtgcttgcc cttaacctga ataaaggctt 540 tttatgacag acaacacaga caatgacaaa ctgtatcgct accttttcca agaccgcgcg 600 gtgcgcggtg aatgggtacg gttaaaccaa acgtttactg atacgttaaa tacacatcaa 660 9 136852·序列表.doc

1 SI 1334884

tatccgaaag tcatccaaaa cttgctcggt gaaatgatgg tggcgaccag tttattgacg 720 gcgacgttaa aatttgaagg ggatattact gttcaagtac aaggtgatgg accattaaaa 790 ttagcattag ttaatggcaa tcatcagcag caaattcgcg cattagcgcg tttacaagcg 840 gatgtcagtg atgatatgag tttggcgcaa ttagtcggga aaggggtatt agtgattacg 900 attgcaccga cagaaggcga gcgttaccaa ggcgtgattg cgttagataa gccaacjatt 960 actgcctgtc ttgaagatta ttttgtgcgt tcagaacaat tgcaaaccca gcttattatt 1020 cgtgctggcg aatttgaagg acaacctgtg gcagccggta tgttgttaca aattatgccg 1080 gacggttcag gttctccaga ggattttgaa cacttagcaa cattggcagc gacggtgaaa 1140 gaggaagaac tatttggttt aacagcagaa gaattattgt accgtttata tcatgaagag 1200 cgtgttgaaa ttttcccttc acagccgatc tcatttttct gtggctgctc acaagaacgt 1260 tctggtgccg cattgttgtt gatttctgat gaagaattgg atgaagtctt ggcagagcat 1320 aacggtacca ttgatatgca gtgtgaatgt tgtgggacgc attatttctt caataaagca 1380 gcgattatgc aattgaaagt agaaaaataa gtcttggtat tatggatttt gttgcaggct X440 atcaagatat ttgatagcct gtttttcctt tgcagcaaac gattttatga gaaaaacgcc 1500 gtcttctcac acagtagttt aggtattgca tagcatgaag cgggaaacta tgtttggcgc 1560 ttgtctgttt aaaaaggtta tctatgttag cactttcacc atcattactt gaaaaaacac 1620 ttgaaatcgc ggatcaagca ggagattttt taacgcgatt ttataccgac tcagggcaaa 1680 atgcgttagc aattcaaacc aaacaagata acacgccagt gactgccgtg gatttatttt 1740 tgagtcaatt tttaattgaa aaattgaccg cactgacacc tgaggtcccg attctttccg 1800 aagagagttg taaaatcccc ttacaagatc gtgcacattg ggcagaatat tggttaattg I860 acccactcga tggaacccaa caatttatta atcgcaccga tcaattttcc attttgatta 1920 cacttgtcca acacaatcaa cccgtcttaa gcattaccca tgcccctatt ttacaaacga 1980 cctattatgc tatgcagggc tttggtgctt acagaaggca gggcaatcaa caagaaaagc 2040 taaacaacca agcgcgacct gagcagcgaa aaatcaaaat tgctgtggga gtcgggggcg 2100 ttgaacggaa aattcagccc ttgttaaatc cagcttatca atatgaattt ttggtttatg 2160 gttcaagtgg cttaaaaggc ggcttagtgg ccgatggcac ttgtgattgc tatattcgag 2220 taggaaaaac gggtgaatgg gatacggggg cggctgaaat cctcttacgt gaaatgggag 2280 gtgctgtgtt tgactttgct tttcaaccgc ttagctataa tcagagagaa agttttatta 2340 atcccaattt tgtgatggta gcgaatacag aatttgattg gcagaaaatt tttcaatttc 2400 attcgcacta ggcattattt attataagat gcgatatctt atgacctcta tttttaacaa 2460 acggatttca ggaaaaaaat gaaaattgaa gcagacaaca attgtattgt aatttttggt 2520 gcatcaggtg atttaactta tcgtaaattq attcctgcac tqtataactt atataaaatc 2580 -10- 136852-序列表.doc tsi 1334884 ggtcgtttga ctgagcattt ctccgtgtta ggtgtggcaa gaacggaatt aagtgatgag 2640 ggtttccgtg aaaaaatgcg ccaagcgttg atcaaaagtg aaaaagcgaa tggcgaaaca 2700 ctcgatcaat tttgtagcca cctttattat caggcattaa ataccgcgga tgctgccgat 2760 tatggcaagt taattcctcg tcttgatgac ttacatgata aatatcaaac ttgtggtaac 2820 acactttact atttatctac gccgccaagc ctttatggcg tgattccaga atgccttgcg 2880 gcacatgggt taaatactga agagtttggc tggaagcggt taattgtgga aaaaccgttt 2940 ggttatgata tacgcacggc aaaagaactc gatattcaaa ttcaccgttt ctttgatgaa 3000 caccaaattt atcgtattga ccactatctt ggtaaagaaa cggttcaaaa tctgcttgtg 3060 ttgcgttttt ctaatggatg gtttgaacca ctctggaacc gtaatttcat tgattatatt 3120 gaaatcacgg gcgcagaatc tatcggtgta gaagagcgtg gtggttatta cgatgattct 3180

ggcgcaatgc gtgatatgtt ccaaaaccat ttgttgcaag tgttagccat ggttgcgatg 3240 gagccaccag caattattaa tgccgactca atgcgtgatg aagtggcaaa agtcttgtat 3300 tgtttacatc cattaagtga ggatgactta gaaaatcatt tagtcttagg gcaatatacg 3360 gcaggcacag ttgaaggtga agcggttaag ggctacttac aggaaaaagg tgtgccggca 3420

gagtctaata cggaaactta catggcatta cgttgtgaaa ttgacaactg gcgttgggcg 34BO ggtgtgccat tttatgtgcg tactgggaaa cggttaccaa gtcgagtgac cgaaattgtg 3540 attcatttca aaaccacacc acatccggta tttagccaaa aagcaccaga aaacaaatta 3600 attatccgta ttcaacccga tgaagcgatt tcgatgcgtt ttggtttgaa aaaaccggga 3660 gcaggttttg aagcaaaaga agtgtcgatg gatttccgtt atgcggatct tgcctcacca 3720 agcttactga ccgcttatga gcgtttatta ttggattcta tgaaaggcga tgccactttg 3780 tttgcgcgta ctgatgcggt acatgcctgt tggcagtttg tggagccgat tttacaatat 3840 aaagcacaaa atgggcgtgC ttatgagtat gaagccggta cttggggacc gacagaagcc 3900

gacaaactga tcgcgaaaac gggtcgtgtt tggcgtaaac caagtggatt aatgaaaaag 3960 aaagtgtaat gtccgcctct ttcgtaagaa atgcgagggg ctaatgtgag cagattgagt 4020 aaggaaagat c atg aat aca ate at仁 ttt gac agt gca cag cat gee gta 4070 Met Asn Thr lie lie Phe Asp Ser Ala Gin His Ala Val 15 10 gag aaa att gca Glu Lys lie Ala 15 gtg cat att tcc Val His lie Ser 30 act tta get caa Thr Leu Ala Gin tat age tta gaa Tyr Ser Leu Glu 25 aeg ccg aaa ttg Thr Pro Lys Leu 40 gag att caa tgg Glu lie Gin Trp 55 caa gaa ttg tta geg Gin Glu Leu Leu Ala 20 tta tcc gga ggc tea Leu Ser Gly Gly Ser 35 gca ccg tat aac acc Ala Pro Tyr Asn Thr 50 ggt ege cct 4118 Gly Arg Pro tta ttt aaa 4166 Leu Phe Lys 45 aaa aat ttg 4214 Lys Asn Leu 60 -II- 136852-序列表.doc

l SI 1334884 cat ttt tgg tgg ggc gat gat cgt atg gtg cca cca acc gat cca gaa 4262

His Phe Trp Trp Gly Asp Asp Arg Met Val Pro Pro Thr Asp Pro Glu 65 70 75 agt aat tac ggc gag gtg caa aaa ttg tta ttc gat cat att cag ate 4310

Ser Asn Tyr Gly Glu Val Gin Lys Leu Leu Phe Asp His lie Gin lie 80 85 90 cct gca gaa aat att cac ege att cgt ggt gaa gcc ccc gtt gag agt 4358

Pro Ala Glu Asn lie His Arg lie Arg Gly Glu Ala Pro Val Glu Ser 95 100 105 gaa ett cac cgt ttt gaa caa geg eta agt geg gtc att cct ggg caa 4406

Glu Leu His Arg Phe Glu Gin Ala Leu Ser Ala Val lie Pro Gly Gin 110 115 120 125 gtt ttt gat tgg att att ttg ggc atg gga aeg gac ggg cac aeg gcc 4454

Val Phe Asp Trp lie lie Leu Gly Met Gly Thr Asp Gly His Thr Ala 130 135 140

tea tta ttc ccg cat caa acc gat ttt gac gat cct cat ttc gcc gtg 4502

Ser Leu Phe Pro His Gin Thr Asp Phe Asp Asp Pro His Phe Ala Val 145 150 155 ate geg aaa cac cct gaa aca ggg caa att cgt att tea aaa aca geg 4550 lie Ala Lys His Pro Glu Thr Gly Gin lie Arg lie Ser Lys Thr Ala 160 165 170 aaa ttg att gaa caa gca aag egg gtg acc tat ttg gtg aca ggt age 4598

Lys Leu He Glu Gin Ala Lys Arg Val Thr Tyr Leu Val Thr Gly Ser 175 180 185 agt aaa gcc gag ate tta aaa gaa att caa act act ccg gca gaa caa 4646

Ser Lys Ala Glu lie Leu Lys Glu lie Gin Thr Thr Pro Ala Glu Gin 190 195 200 205 ctg cct tat cct get geg aaa ate aaa geg aag cat ggg gtg aeg gaa 4694

Leu Pro Tyr Pro Ala Ala Lys lie Lys Ala Lys His Gly Val Thr Glu 210 215 220 tgg tat ttg gat aag gat geg gca aaa tta ctg taatgegteg tgagattttt 4747

Trp Tyr Leu Asp Lys Asp Ala Ala Lys Leu Leu 225 230

caacattttt gcaaagagac ttgaaacaaa atagaccata gcgttcgttt tcaacgagtg 4807 ctgaaaatga aggetetegt tgaaaatggc gccatttagt gggtaagctt aaggttcgct 4867 cagacagcgc tatcaaaagg gtaaaagaat gtatcaactc tattttaatc caccaaaatc 4927 ttcatggtca ttacgcgttt gggtgttatt gaaagaactg gcaattcctt ttgagcctaa 4987 aattgtacgt tatttggatg atttaagtga acaacgccaa caatttaagg cgttttcgcc 5047 gacttcaaaa atcccagtat tgcatgctga tggtgttgtc atttgggaca gtttagcgat 5107 tategagett ttggcagaaa gttatccgca cgtgtgggcg caagataagg cgacaagagc 5167 gtggtcacgt tctgcttgtg ctgaaatgca ctctggcttt gaaaatttgc gtgaaatgtg 5227 tgatttcgcc cctttagctc gcaaaccgtt acaagaaatg cccgctgtgt taagccaaga 5287 gctaacaagg ettaaccaat tattagaaga agggttaaca aatcacactg ggcgatttaa 5347 -12- 136852-序歹[(表.doc 1334884

tgcaaacctc cagtgctttt ggtcgagtac aatcctcgtt gtcttccttc cgtaattcct 5407 atgatgactt tgccgcttat cgtgcggtgt taaatcgttt aactggcttt catagcgcga 5467 ttaaagcggc taatcgccca agcaaattag tgctcacaga aagtcgtcat gatattggtt 5527 ttcatgatct caacgtagag accccgcaag gaaaaacctt aattgacaag ctcaacctac 5587 aatttcctct cggtacatgg ttattaattc aaggacattc tggtgtaggg aaaacaacct 5647 tgttaagaac cattgcggga ctatggcctt atgctagtgg gacaattcaa cgtccacaac 5707 aagatactct gtttctttct caaaaacctt atttgccaca aggtcgccta cttgatgccc 5767 tattttatcc tgaactggcg cctgaagacg tgaatgagca acaagttata gacatactcg S827 cgaaagtaca actcgggcat ttaagcgaCa aactagaaca agaaaatgat tggacacggg 5887 tactctcttt aggtgaacaa caacgtctgt cgtttgctcg cattttattg cataaaccta 5947 ctgttgtttt cttagatgaa gccactgcca gcatggatga aggactggaa gatgcgatgt 6007 accgcttact gaaagatgaa ctgcctcaga ttactgtgat cagtgttgga caccgttcga 6067 cgttaattcc gcaccattca cagcaattac acattcaata acaagacagg gcgttgtgga 6127 gtcga 6132 <2i〇> 8 <211> 232 <212>蛋白質 <213>敗血性巴斯德样蔺 <400> 8

Met Asn Thr lie lie Phe Asp Ser Ala Gin His Ala Val Glu Lys lie 1 5 10 15

Ala Gin Glu Leu Leu Ala Tyr Ser Leu Glu Gly Arg Pro Val His lie 20 25 30

Ser Leu Ser Gly Gly Ser Thr Pro Lys Leu Leu Phe Lys Thr Leu Ala 35 40 45

Gin Ala Pro Tyr Asn Thr Glu lie Gin Trp Lys Asn Leu His Phe Trp 50 55 60

Trp Gly Asp Asp Arg Met Val Pro Pro Thr Asp Pro Glu Ser Asn Tyr 65 70 75 80

Gly Glu Val Gin Lys Leu Leu Phe Asp His lie Gin lie Pro Ala Glu Θ5 90 95

Asn lie His Arg lie Arg Gly Glu Ala Pro Val Glu Ser Glu Leu His 100 105 110

Arg Phe Glu Gin Ala Leu Ser Ala Val lie Pro Gly Gin Val Phe Asp 115 120 125

Trp lie lie Leu Gly Met Gly Thr Asp Gly His Thr Ala Ser Leu Phe 130 135 140

Pro His Gin Thr Asp Phe Asp Asp Pro His Phe Ala Val lie Ala Lys -13· 136852·序列表,doc

l SI 1334884 145 150 155 160

His Pro Glu Thr Gly Gin lie Arg lie Ser Lys Thr Ala Lys Leu lie 16S 170 175

Glu Gin Ala Lys Arg Va.l Thr Tyr Leu Val Thr Glv Ser Ser Lys Ala 180 185 190

Glu lie Leu Lys Glu lie Gin Thr Thr Pro Ala Glu Gin Leu Pro Tyr 195 200 205

Pro Ala Ala Lys lie Lys Ala Lys His Gly Val Thr Glu Trp Tyr Leu 210 215 220

Asp Lys Asp Ala Ala Lys Leu Leu 225 230 <210> 9 <211> 2438 <212> DNA <213>敗血性C*斯德桿菌 <220>

<221> CDS <222> (1635) . . (2396) <220> <223> dnaA <400> 9 aaaagcgaaa 60 taaagcatta 120 cttaaatgaa 180 cactaaataa 240 gcttacaatc 300 aaacttaaca 360 gccttcgttg 420 attacgggtt 480 caactttgta 540 ccaattcaat 600 actggactgg 660 caaaaagtct 720 gcccctgttg 780 gtattcgttt 840 atgatgaaat 900 ttaggcttaa 960 gacccaatgc ttgccaccgg cggctctatg attgcgacaa tcgatcttct ggctgtaaac acattaaagt gctcgtgtta gtcgccgcgc ctgaaggcat gaagctgcgc accctgatat cgaattatat accgcatcag ttgatagtca caaggctata ttattccagg tcttggtgat gccggtgata aaatttttgg tcccaacaca agcggcatct tatgccgctt ttttccgttc aatttatagc ttaacagctt gaacactata aaatgaaaag ttaattcaga cagagagttg tgacaaatca aaatccccct gttcttctag aacaaaatca cgcaaaacaa ggctacaaat gctttttgtt gccttcggtg ctttagtcct tgttcccctg taaatgccaa tactgcctta ttgaccgcag ggattgggac actcttattc ctggacgcca agtcccaatt ttcttagcct cttcctttgc ttttattgca atggcgtggc aacatggggc attgctacta ctatgggggg gctggtgttt tttattttgc cctcagtacg ttagtcaaaa ttaaaggtgc tggtgcttta ttccgccagt agttgttggt cccgttatta tcatcatcgg catggaactt ccgtggacat ggcattaggt aaaaacagca cttatcaaca taacgatgcc cgatggcaac attattgaca acgttaggtg ttgcggtgct tgctaaaggc taattcctat catgcttggt attgtcgtcg gctatatcct ctgcttattc -14. 136852·序列表.doc I Si 1334884 ttaatttcca acctgtcatt gatgcacctt ggtttagtgt gccagaaatt actacccctg 1020 aatttaaatt agaagctatt ctttatttat taccgattgc tatcgcccca gcagttgagc 1080 atgtcggtgg gatcatggca atcagttcgg tgacagggaa agacttccta caaaaaccag 1140 gattacatcg cactttatta ggggatggta ttgcaacgag tgccgcctca ttcttaggag 1200 gaccacctaa tacaacttat gctgaagtca ctggtgctgt catgcttacg cgcaacttta 1260 accctaaaat catgacatgg gcagccgttt gggcaattgc gatttccttc tgtggtaaag 1320 tcggggcttt cctctctacc attccaacta ttgtcatggg tggcattatg atgttagtgt 1380 ttggctctat cgccgtagtc ggtatgagta cactgatccg tggtaaagtg gatgtaacag 1440 aagcgcgtaa tctgtgtatt atttccgttg tgatgacgtt tggcatcggt ggtatgtttg 1500 tgaactttgg agaagtctcc ttaaaaggga ttagtttatg cgccgttgtc gcgattttac 1560

tcaacttaat cttacccaaa gccaaaaaca ccccaataga agaaaatcga taagagaaaa 1620 ttaagggtta agtc ttg ctt aac cct tea ttt ttc gtt tat cct tat tet 1670

Leu Leu Asn Pro Ser Phe Phe Val Tyr Pro Tyr Ser 1 5 10 cct ttt ttc gat ttt gta ggt tgc ttt ttg tta gaa aat ttc caa tta 1718

Pro Phe Phe Asp Phe Val Gly Cys Phe Leu Leu Glu Asn Phe Gin Leu 15 20 25 cct ttg cct att cat caa etc gat gat gaa aeg ctg gat aat ttc tat 1766

Pro Leu Pro lie His Gin Leu Asp Asp Glu Thr Leu Asp Asn Phe Tyr 30 35 40 ccc gac aat aat tta ttg ttg etc aat teg eta ege aaa aat ttt act 1814

Pro Asp Asn Asn Leu Leu Leu Leu Asn Ser Leu Arg Lys Asn Phe Thr 45 50 55 60 tgt eta aca caa caa ttt ttt tat att tgg ggc gag caa age agt ggt 1862

Cys Leu Thr Gin Gin Phe Phe Tyr lie Trp Gly Glu Gin Ser Ser Gly 65 70 75

aaa agt cac etc tta aaa ggc att act cat cat ttt ttc ctt tta cag 1910

Lys Ser His Leu Leu Lys Gly lie Thr His His Phe Phe Leu Leu Gin 80 8S 90 ege ccc get ate tat gtg ccc tta gaa aaa tcc caa tat ttc tea ccg 1958

Arg Pro Ala He Tyr Val Pro Leu Glu Lys Ser Gin Tyr Phe Ser Pro 95 100 105 geg gta etc gaa aac tta gaa caa caa caa ttg gtt tgt tta gat aat 2006

Ala Val Leu Glu Asn Leu Glu Gin Gin Gin Leu Val Cys Leu Asp Asn 110 115 120 tta cag gca att ata ggc aat act gaa tgg gaa tta geg att ttt gat 2054

Leu Gin Ala lie lie Gly Asn Thr Glu Trp Glu Leu Ala lie Phe Asp 125 130 135 140 tta ttt aat ege ata aaa tet gtt gaa aat aca ctg ctt gtg ate agt 2102

Leu Phe Asn Arg lie Lys Ser Val Glu Asn Thr Leu Leu Val lie Ser 145 150 155 gca aat caa tcc cca act gca tta cct gta age tta cct gac tta get 2150 -15- 136852·序列表.doc 1334884

Ala Asn Gin Ser Pro Thr Ala Leu Pro Val Ser Leu Pro Asp Leu Ala 160 165 170 tea cgt tta ege tgg gga gaa age tat caa ctg gtc ecc tta aac gat 2198

Ser Arg Leu Arg Trp Gly Glu Ser Tyr Gin Leu Val Pro Leu Asn Asp 175 180 185 caa caa aaa ate cat gta ttg caa aaa aat gca cat caa cgt ggt ate 2246

Gin Gin Lys lie His Val Leu Gin Lys Asn Ala His Gin Arg Gly lie 190 195 200 gaa etc ccc gat gaa gta get aat ttt ett ttg aaa ege tta gag ege 2294

Glu Leu Pro Asp Glu Val Ala Asn Phe Leu Leu Lys Arg Leu Glu Arg 205 210 215 220 gat atg aaa aeg tta ttt gaa gca eta agt aaa tta gat aaa gca tea 2342

Asp Met Lys Thr Leu Phe Glu Ala Leu Ser Lys Leu Asp Lys Ala Ser 225 230 235 tta caa gee caa cgt aaa tea aeg att ccc ttt gta aaa gaa att tta 2390

Leu Gin Ala Gin Arg Lys Leu Thr lie Pro Phe Val Lys Glu lie Leu 240 245 250 aag eta taaaaaaaga ccacctcttt atcaggtgat ctttcttgtc ga 2438

Lys Leu <210> 10 <211> 254 <212>蛋白質 <213>敗血性巴斯德桿菌 <400> 10

Leu Leu Asn Pro Ser Phe Phe Val Tyr Pro Tyr Ser Pro Phe Phe Asp 15 10 15

Phe Val Gly Cys Phe Leu Leu Glu Asn Phe Gin Leu Pro Leu Pro He 20 25 30

His Gin Leu Asp Asp Glu Thr Leu Asp Asn Phe Tyr Pro Asp Asn Asn 35 40 45

Leu Leu Leu Leu Asn Ser Leu Arg Lys Asn Phe Thr Cys Leu Thr Gin 50 55 60

Gin Phe Phe Tyr lie Trp Gly Glu Gin Ser Ser Gly Lys Ser His Leu 65 70 75 80

Leu Lys Gly lie Thr His His Phe Phe Leu Leu Gin Arg Pro Ala lie 85 90 95

Tyr Val Pro Leu Glu Lys Ser Gin Tyr Phe Ser Pro Ala Val Leu Glu 100 105 110

Asn Leu Glu Gin Gin Gin Leu Val Cys Leu Asp Asn Leu Gin Ala lie 115 120 125 lie Gly Asn Thr Glu Trp Glu Leu Ala lie Phe Asp Leu Phe Asn Arg 130 135 140 lie Lys Ser Val Glu Asn Thr Leu Leu Val lie Ser Ala Asn Gin Ser 145 150 155 160 16 · 136852·序列表.doc

1 SI 1334884

Pro Thr Ala Leu Pro Val Ser Leu Pro Asp Leu Ala Ser Arg Leu Arg 165 170 175

Trp Gly Glu Ser Tyr Gin Leu Val Pro Leu Asn Asp Gin Gin Lys lie 180 185 190

His Val Leu Gin Lys Asn Ala His Gin Arg Gly lie Glu Leu Pro Asp 195 200 205 olu Val Ala Asn Phe Leu Leu Lys Arg Leu Glu Arg Asp Met Lys Thr 210 215 220

Leu Phe Glu Ala Leu Ser Lys Leu Asp Lys Ala Ser Leu Gin Ala Gin 225 230 235 240

Arg Lys Leu Thr lie Pro Phe Val Lys Glu lie Leu Lys Leu 245 250

<210> 11 <211> 2060 <212> DNA <213>敗血性巴斯德桿菌 <220>

<221> CDS <222> (056)..(1389) <220> <223> dsbB <400> 11 gaattcttct tacgtatgct cccagtcacg ttgccagttc tcatttgtgg tttagtgacc 60 tgcttcttag tggaaaaatt tggtgtattt ggctatggcg ccaaattgcc acgtaaagta 120 tggggcatct tggcaaagtt tgatcgcaat aatcaacaaa aaatgtcacg acaagatcgt 180 ttgaaacttt ttgtgcaagc tttaattggt atttggttgg ttgttggact cgcattccat 240 ctcgccgccg tcggtatcat tggtttaacg gtgattattt tggctacttc attttgtggt 300

gtcaccagcg agcatgctat tggtaaagcc tttcaggaat ccttaccctt cacagcattg 360 ttagtggtgt tcttctcggt tgttgccgtc atcattgacc aacatctgtt tgcgccaatt 420 attcagtttg tgctggctgc cagtgaacat actcagcttg ctcttttcta tatttttaac 480 ggtttgttat ccgccatttc agataatgtg tttgtggcca cagtttatat caatgaaacc 540 aaagcggcat tagaggctgg cttaattgct caaccacaat atgaattact ggcagtagca 600 attaataccg gtaccaatct tccttctgtt gcaaccccaa atggtcaagc cgcattctta 660 tttttattga cctcatcact ggcaccatta attcgtcttt cttatggtag aatggtttat 720 atggcattgc cttataccat cgtattatcc tgtattggtt tattgactgt ggaatatatt 780 ttgcctggcg caaccaatgt gctcattcaa attggtttat taaaaccaat gtaatgacaa 840 gtaaaaggag gaaac atg eta age ttt ttt aag aca etc tea aca aaa ega 891 Met Leu Ser Phe Phe Lys Thr Leu Ser Thr Lys Arg 15 10 -17- 136852·序列表.doc

1 SI agt gca tgg ttt eta ttg ttt age tea get tta eta tta gag get ate 939

Ser Ala Trp Phe Leu Leu Phe Ser Ser Ala T.eu Leu Leu niu 為 lie 15 20 25 get ett tat ttt caa cat ggc atg ggg etc gee cct tgt gtc atg tgt 987

Ala Leu Tyr Phe Gin His Gly Met Gly Leu Ala Pro Cys Val Met Cvs 30 35 40 att tac gag agg gta get att ett ggc att get ttc tee ggt tta ttg 1035 lie Tyr Glu Arg Val Ala lie Leu Gly lie Ala Phe Ser Gly Leu Leu 45 · 50 55 60 ggg tta etc tac ccg agt teg atg ett ttg ege ett gtg geg tta tta 1083

Gly Leu Leu Tyr Pro Ser Ser Met Leu Leu Arg Leu Val Ala Leu Leu 65 70 75 att ggt tta age agt gca ate aaa ggc tta atg att age ate acc cat 1131 lie Gly Leu Ser Ser Ala lie Lys Gly Leu Met lie Ser lie Thr His 80 85 90 tta gat eta caa ett tac cct gca cct tgg aaa caa tgt tea geg gtg 1179

Leu Asp Leu Gin Leu Tyr Pro Ala Pro Trp Lys Gin Cys Ser Ala Val 95 100 105 gca gaa ttt ccc gag act tta ccc tta gat cag tgg ttt cct gca etc 1227

Ala Glu Phe Pro Glu Thr Leu Pro Leu Asp Gin Trp Phe Pro Ala Leu 110 115 120 ttc etc cct tea ggc tea tgc agt gaa gta aca tgg caa ttt etc ggc 1275

Phe Leu Pro Ser Gly Ser Cys Ser Glu Val Thr Trp Gin Phe Leu Gly 125 130 135 140 ttt tet atg gtg caa tgg ate gtc gtc att ttt gca etc tat acc tta 1323

Phe Ser Met Val Gin Trp He Val Val lie Phe Ala Leu Tyr Thr Leu 145 150 155 tta ett get etc att ttc ate age caa gtc aaa cgt eta aaa ccc aag 1371

Leu Leu Ala Leu lie Phe lie Ser Gin Val Lys Arg Leu Lys Pro Lys 160 165 170 cag ege aga etc ttt cat taagtcataa aaaatggtgc gataaageae 1419

Gin Arg Arg Leu Phe His 175 catttttcat ttctcgttcg gtatagatta aatttcttgc acgacaaact gcagggaatg 1479 tettgetaat aatettgett taegetgett taagcgtttt aaacgtaatg cgcgtccttg 1539 tgacaaacga tataattttt ttgtcggctt caaaaagctt ttaegteteg cca-actctt 1599 cctcttattg tgtcgttacg gttgtcgcta acgtatccgt ttctttcttg attrcgttga 1659 tgttttctat tteggegget tgtgtatcta atgtttctcc aatttgtgct tgcrgtaccc 1719 gataataatg cacaatgctg ttcatataac gaeggatatt ttccacatag tgatatgett 1779 catagcctcg cgcatacccg tattttaacc ccgtgtaata gcgtttttct getaatagtg 1839 gcaaattctt cttcacatct aaccaattat cgggatcacc acctaggctc ttcgttaaac 1899 ggcgcgcatc taacaaatgc cctaatccca tattataege egetaaggea aacraaatac 1959 gctcatcttc tttaatcgta tcaqgcattt qcqtaataaa ccaatacaaa ta:*:ctqaac 2019 -18· 136852-序列表,doc i S3 1334884 20^0 cggctttaat actttgttcc ggatccgttc tgtcttgaat t <210> 12 <211> 178 <212>蛋白賀 <213>敗血性巴斯德样菌 <400> 12

Met Leu Ser Phe Phe Lys Thr Leu Ser Thr Lys Arg Ser Ala Trp Phe 15 10 15

Leu Leu Phe Ser Ser Ala Leu Leu Leu Glu Ala lie Ala Leu T/r Phe 20 25 30

Gin His Gly Met Gly Leu Ala Pro Cys VaX Met Cys lie Tyr Glu Arg 35 40 45

Val Ala lie Leu Gly lie Ala Phe Ser Gly Leu Leu Gly Leu Leu Tyr 50 55 60

Pro Ser Ser Met Leu Leu Arg Leu Val Ala Leu Leu lie Gly Leu Ser 6S 70 75 80

Ser Ala lie Lys Gly Leu Met lie Ser lie Thr His Leu Asp Leu Gin 85 90 95

Leu Tyr Pro Ala Pro Trp Lys Gin Cys Ser Ala Val Ala Glu Phe Pro 100 105 1X0

Glu Thr Leu Pro Leu Asp Gin Trp Phe Pro Ala Leu Phe Leu Pro Ser 115 120 125

Gly Ser Cys Ser Glu Val Thr Trp Gin Phe Leu Gly Phe Ser Met Val 130 135 140

Gin Trp lie Val Val He Phe Ala Leu Tyr Thr Leu Leu Leu Ala Leu 145 150 155 160 lie Phe lie Ser Gin Val Lys Arg Leu Lys Pro Lys Gin Arg Arg Leu 165 170 175

Phe His

<210> 13 <211> 4426 <212> DNA <213:>敗血性巴斯德样菌 <220>

<221> CDS <222> (2756)..(3211) <220> <223> exbB <400> 13 gaattcttga cctggtgtga ggctcttatt gacttccacc ccaattgcgc gttctttcac 60 tttattaata aagtcacgta ccactggtaa cgccacatcc gcttctaata atgccatgcg 120 •19· 136852-序列表.doc 1334884 gacttcacgt agcgtttcct taatattgtc atcagttaaa cgtccacgtc cactaatgtt 180 acgtagcgcc tttgacaagc gatccgacaa attctcaaac atgcaatctt cctatttaat 240 ctcgaaaaaa ttgccctaat tatactgaaa tcccatagat tttcatcctt tgtaataaat 300 ttatagcgat ttcacagtga gaaagttaga atggacggag aacaaaggtg agggaatttc 360 aatgtggctt tcaatttttt ccgtgttttt ttatctattg agtgtcttac tcattacccc 420 tatgttgtta aaaattcaag cgggcgaacc cgcatcggtt cccaatagga tgcctttttt 480 aacggcagca ttactcgcca ttcttctgca ctttatcaat cttattcctc tttttaccga 540 tttaaccagc ggtcaaaatt tctcggttat cgaaatcagt tccttaatca gtgttatgac 600 agcaacgatc gccacattag cgtttttatt tcgcatacac actttatggt ttttattacc 660 gattatttat tctttcgcca tcattaatct ggtcctcagt accttaatgc cggcgcattt 720

tttatatcat cttaatcaag atatgggctt atttattcat gtaggcttat cactgttgac 780 ttattcggtc tgttcgattg tcgcgttata ttccattcag ttagtttgga tcgatcgcgc 840 cttaaaaaat aaaaaactgc ctttttcacc aatgattcca ccgttaatga ccgtagagcg 900 ccattttttt cgtttaatgc tgataggtga aatattactc acgattgtcc tcatttcagg 960 gagctaccat ttatcgaaaa cctttgcccc acaagacatc cagaaagctg ttttttcttt 1020 cttggcttgg atagtctttg ggagtgcact ggttggtcat tggaagctcc attggcgtgg 1080 aaaaaaagtg gtgtattatg cgcttttagg tatcattctc ctgactatcg cctattttgg 1140 tagtcgtgta atgcttgaaa tataaacaag atttatttat gctacgccat agtaaaagtg 1200 cggttcaaaa aacgaaaaaa tgaccgcact tttcgatttt tgattaactc gcaaaaggat 1260 gcattttgga cagtattccc cttagtaccc tctttattac actcatcatc ttattgatca 1320 tctctgctta tttttcaggc tctgaaaccg gtttgttgtc tgccaatcgt tatcgactac 1380

gccatttagc cgaaaaaggg catagaggtg cgaaaaaagc ggaaaaatta ctgaaaaaaa 1440 cagatgtttt gcttagcctg attttaattt gtaataactt agtcaatatt actgcttctg 1500 ccattaccac gattatcggc atgcgcttat atggcgatgc gggagtcgcc attgcaactg 1560 gggcattaac ctttgtgatg cttatttttg ccgaaatttt gccgaaaact atcgccgccc 1620 gttatccaga aaaagtggca ttcacgtcca gtcatttgtt gtccgttttt ctgcgacttt 1680 ttaccccgct ggtctattta atgaatttaa ttattcaggg gattttggca ctattacgtc 1740 taaaatcaga taataaatca acctcattaa gcccagagga attacgttcc atcgtaaatg 1800 aatcaggtaa atttattcct tccgcccacc aagaaatgct gttatctatt ttggatttag 1860 aaggggtcac cgtagatgac attatggtgc cacgtaatga cattgggggt attgatattg 1920 acgatgattg gaaagccatt atgcgtcaac ttaaccatgc agcgcacggg cgtgttgtac 1980 tgtataaagg aaatatggat gaaaatattc tggggatgtt acgtgtacgt gaagcctatc 2040 -20- 136852-序列表.doc is] gcttaatgct cgataaaaat gaatttaaca aagaaacttt aatccgtgcc gccgatgaag 2100 tgtattttat tcctgaaggt acgccactga atagccaatt attaaatttc cgcaacaata 2160 aagaaaggat tggtttagtt gtagatgaat atggtgatat taaaggctta gtcaccttag 2220 aagatatctt agaagagatt gtcggtgaat ttaccacttc aacagcccca tcaattaacg 2280 atgaagttat cccacaatca gacggttcgc ttatcattga gggatccgcc aatttacgtg 2340 atttgaataa attatttgac tggaatctcg ataccgaaga tgcacgtacc ttcaacggct 2400 taattttaga gcatttagaa gaaattccag aagaaggaac ggtatgtgaa attaatgggc 2460 tacaaatcac gattctagaa gtgaatgaca acatgattaa acaagccaaa gtcattaaac 2520 tttaattcaa catctggcta agcgatgtca tcaagacatc gcttttttat tccgtacatg 2580 aatgtttgat ccaacacaac atttattcaa cattggataa ataatcatcc taaatcgcac 2640 gaatttctta tttaccccgt ttttggcttt tgctagaatc ttgcaattga aattaattct 2700 caataccgta taatgttcaa cattattttg cgatacaaat taaaggatta ttaaa atg 2758

Met 1 cca caa tta ttc caa ttc tta caa caa tat att gat tac att att eta 2806

Pro Gin Leu Phe Gin Phe Leu Gin Gin Tyr lie Asp Tyr lie lie Leu 5 10 15 ggc tta ett gcc ttc atg age ttt att atg gtt tgg ett gtg att gaa 2854

Gly Leu Leu Ala Phe Met Ser Phe lie Met Val Trp Leu Val lie Glu 20 25 30 ege ttt ett ttc tta agt ege gtc aac gtg gca tet tat gaa age ata 2902

Arg Phe Leu Phe Leu Ser Arg Val Asn Val Ala Ser Tyr Glu Ser lie 35 40 45 cat gaa tta gac att gac tta caa ege cac etc aca get ate tet aca 2950

His Glu Leu Asp He Asp Leu Gin Arg His Leu Thr Ala lie Ser Thr 50 55 60 65 ate ggt tet aat gca cct tat gta ggt ttg ett ggt acc gtc att ggt 2998 lie Gly Ser Asn Ala Pro Tyr Val Gly Leu Leu Gly Thr Val lie Gly 70 75 80 att etc tta act ttc tat gaa tta ggt cac tee ggt ggc gat att gat 3046 lie Leu Leu Thr Phe Tyr Glu Leu Gly His Ser Gly Gly Asp lie Asp 85 90 95 geg geg gcc att atg gtg cac tta tea tta gcc tta aaa gcc aca gca 3094

Ala Ala Ala lie Met Val His Leu Ser Leu Ala Leu Lys Ala Thr Ala 100 105 110 gta ggt att tta gtc gcc att cct gca atg gtg tgt tac aac ggt tta 3142

Val Gly lie Leu Val Ala lie Pro Ala Met Val Cys Tyr Asn Gly Leu 115 120 125 gga cgt aaa gtc gaa gtt aat cgt ttg aaa tgg ttt gcc tta aat gag 3X90

Gly Arg Lys Val Glu Val Asn Arg Leu Lys Trp Phe Ala Leu Asn Glu 130 135 140 145 aaa aaa gcc aaa caa caa gca tagggagccg tcatgaaaaa gtttgatgaa 3241 •21 - 136852-序列表.doc t si 1334884 hys hys A上a Xjys G丄II Gin A上a 150 atcaacatta tcccttttat tgacatcatg ttggtactat tggctatcgt tctgattaca 3301 gcctctttta tttcacaagg taaaatccaa gtgaatgtac caaaagcaag ttcaacagtt 3361 gcgtttcgtt cagatgattt agccaaattg ctgactatta cggaaagcgg tgaaattttt 3421 tatcacgata aaccgattac gatagaggca ttggaacaag aaatcagtaa ttgggaaaaa 3481 gatcaaaaag tcaccttgaa ggtagatgca aaatccagtt tccaagatct cgtttctatc 3541 actgatatta tggctaaaaa tgaaattaaa aatgtcgcta tcgtgacggt taaagaaaag 3601 gcatctcaat gatagataaa agtcgttctt gcatcgggtt tgcaatttca ttgctttttc 3661 acgcaagttt tgtctctttc ctgtattgga ttgtacaaaa agacgatgac agcgcgaatg 3721 gatttgctgc cgatatcatc tcaactcata tttccatgga aatgctggcg gctaccgttt 3781

tagaagaacc agagccggaa ccagagccgg cgcctccggt agtagaacct gaactgccaa 3841 aagaagtagt cgcagatccg acggtaaaac ctgagccacc aaaagaaccc gaaaaaccaa 3901 aagagcctga aaagccaaaa gagaaaccga aagaaaaacc aaaagaaaag ccgaaaaaac 3961 cgaagaaaga acaacgtgat ttaccaaagt cagatcgcca aattgattct aattcatcga 4021 tcaatcaaca agcgaccaca acaggcaaca tcacaaccaa taatccgaat ctggtcggta 4061 aaggtaatag cacagatgaa gtcaatgctt atcgctcggc tttacgcaga gaaattgaaa 4141 aacataaacg ctatccaaac cgtgcacgca tgatgcgcaa acaaggtgtg gtaacaatca 4201 cgttccatct taataatgcc ggcgtaatta gtaatgcgcg aatcagcaaa tcttccggct 4261 cagaagaatt agataacgct gcactggtag ctgtcaataa tgcccgtcca attggtccac 4321 tgcctgttgg tatgccaaat gaagtgagcg ttcctgtcag tttcagaatc acaaattaaa 4381 aaagtgcggt aaaatttacc gcactttttt ctctctatta gaatt 4426

<210> 14 <211> 152 <212>蛋白質 <213>敗血性巴斯德桿菌 <400> 14

Met Pro Gin Leu Phe Gin Phe Leu Gin Gin Tyr lie Asp Tyr lie lie 1 5 10 15

Leu Gly Leu Leu Ala Phe Met Ser Phe lie Met Val Trp Leu Val lie 20 25 30

Glu Arg Phe Leu Phe Leu Ser Arg Val Asn Val Ala Ser Tyr Glu Ser 35 40 45 lie His Glu Leu Asp He Asp Leu Gin Arg His Leu Thr Ala lie Ser 50 55 60

Thr lie Gly Ser Asn Ala Pro Tyr Val Gly Leu Leu Gly Thr Val lie •22· 136852-序列表.doc isi 1334884 65 70 75 βΟ

Gly I上e Leu Leu Thr Phe Tyr Giu Leu G上y His Ser G丄y Gly Asp lie 85 90 95

Asp Ala Ala Ala lie Met Val His Leu Ser Leu Ala Leu Lys Ala Thr 100 105 110

Ala Val Gly lie Leu Val Ala lie Pro Ala Met Val Cys Tyr Asn Gly 115 120 125

Leu Gly Arg Lys Val Glu Vai Asn Arg Leu Lys Trp Phe Ala Leu Asn 130 135 140

Glu Lys Lys Ala Lys Gin Gin Ala 145 150

<210> 15 <211> 6876 <212> DNA <213>敗血性巴斯德样菌 <220>

<221> CDS <222> (534) . . (6863) <220> <223> fhaBl <400> 15 agatgcgtga tctgatcctt caactcagca aaagttcgat ttattcaaca aagccgccgt 60 cccgtcaagt cagcgtaatg tctgccagtg ttacaccaat taaccaattc tgattagaaa 120 aactcatcga gcatcaaatg aaactgcaat ttattcatat caggattatc aataccatat 180 ttttgaaaaa gccgtttctg taatgaagga gaaaactcac cgaggcagtt ccataggatg 240 gcaagatcct ggtatcggtc tgcgattccg actcgtccaa catcaataca acctattaat 300 ttcccctcgt caaaaataag gttatcaagt gagaaatcac catgagtgac gactgaatcc 360

ggtgagaatg gcaaaagctt atgcatttct ttccagactt gttcaacagg ccagccatta 420 cgctcgtcat caaaatcact cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga 480 gcgagacgaa atacgcgatc gctgttaaaa ggacaactac aaacaggaat cga atg 536

Met 1 caa ccg gcg cag gaa cac tgc cag cgc ate aac aat att gtt aac caa 584

Gin Pro Ala Gin Glu His Cys Gin Arg lie Asn Asn lie Val Asn Gin 5 10 15 gaa aac ggt tta ttc cat aca etc ggt aat atg atg tta gaa gca gag 632

Glu Asn Gly Leu Phe His Thr Leu Gly Asn Met Met Leu Glu Ala Glu 20 25 30 cgt Cct gtt tat aat att ggc gat att tat gcg agt aaa aaa tta aca 680

Arg Ser Val Tyr Asn lie Gly Asp lie Tyr Ala Ser Lys Lys Leu Thr 35 40 45 •23· 136852-序列表.doc isi 1334884 gtt cat act cat aat ttg att aat gat gtg cgt tta tct ggc aat gtg 728

Val His Thr His Asn Leu lie Asn Asp Val Arq Leu Ser Glv Asn Val 50 55 60 65 agt tat aag cct ate ggt tea agt cgt gat tat gat ate agt cgt gtt 776

Ser Tyr Lys Pro lie Gly Ser Ser Arg Asp Tyr Asp lie Ser Arg Val 70 ' 75 80 geg gta cat ggt tgg cac aat aat gtt tat aag etc aac tta aat ctg 824

Ala Val His Gly Trp His Asn Asn Val Tyr Lys Leu Asn Leu Asn Leu 85 90 95 caa gaa caa gat aaa acc gat att aaa gtt gtg aaa atg ggg get ate 872

Gin Glu Gin Asp Lys Thr Asp lie Lys Val Val Lys Met Gly Ala lie 100 105 110 cgt tct gat ggt gat ttt gac ttt aag gga ata aag geg aca tea tea 920

Arg Ser Asp Gly Asp Phe Asp Phe Lys Gly lie Lys Ala Thr Ser Ser 115 120 125

gaa tea aaa ccg cag tta att aat cat gga tta att aat gtc aaa gga 968

Glu Ser Lys Pro Gin Leu lie Asn His Gly Leu lie Asn Val Lys Gly 130 135 140 145 aca ttt aat geg gaa get gat caa gtg gtg aac caa atg aaa geg ttc 1016

Thr Phe Asn Ala Glu Ala Asp Gin Val Val Asn Gin Met Lys Ala Phe 150 155 160 aac caa aat gca tta gca age gtg ttt aag aat cca geg aaa ate aeg 1064

Asn Gin Asn Ala Leu Ala Ser Val Phe Lys Asn Pro Ala Lys lie Thr 165 170 175 atg tac tat caa cca ett act cgt tat att tgg aca cca tta teg ggt 1112

Met Tyr Tyr Gin Pro Leu Thr Arg Tyr lie Trp Thr Pro Leu Ser Gly 180 1B5 190 aat gca teg cgt gaa ttt aac aat tta gag tct ttc etc gat gee ttg 1160

Asn Ala Ser Arg Glu Phe Asn Asn Leu Glu Ser Phe Leu Asp Ala Leu 195 200 205 ttt ggc tea aca aca ate tta aaa tea agt ttc tat agt aeg gaa aat 1208

Phe Gly Ser Thr Thr lie Leu Lys Ser Ser Phe Tyr Ser Thr Glu Asn 210 215 220 225 ttt agt get tat cag ett eta tct cat att cag cat tea cca atg tac 1256

Phe Ser Ala Tyr Gin Leu Leu Ser His lie Gin His Ser Pro Met Tyr 230 235 240 caa aaa geg atg gca caa gtg ttt ggt gca gag tgg cat agt aaa tee 1304

Gin Lys Ala Met Ala Gin Val Phe Gly Ala Glu Trp His Ser Lys Ser 245 250 255 tat gat gag atg ega aac aaa tgg aaa age ttt aaa gaa aat cca aca 1352

Tyr Asp Glu Met Arg Asn Lys Trp Lys Ser Phe Lys Glu Asn Pro Thr 260 265 270 gat ttc att tat tac cca tea gaa aaa gca aaa ate eta geg gga aaa 1400

Asp Phe He Tyr Tyr Pro Ser Glu Lys Ala Lys lie Leu Ala Gly Lys 275 280 285 eta gaa ggt aag ett aca aeg eta caa aat ggt gaa tat gee gaa cgt 1448

Leu Glu Gly Lys Leu Thr Thr Leu Gin Asn Gly Glu Tyr Ala Glu Arg 290 295 300 305 -24- 136852-序列表.doc 1334884 ggt aag ttt gat gag agt ate caa att ggt aaa cac caa tta teg eta 1496

Gly Lys Phe Asp Glu Ser lie Gin lie Gly Lys His Gin Leu Ser Leu 310 315 320 cca tea gta gag ett aaa geg gag ttt agt gat aaa gaa cgt ttg gaa 1544

Pro Ser Val Glu Leu Lys Ala Glu Phe Ser Asp Lys Glu Arg Leu Glu 325 330 335 gag gac ggg gta gat tta tcc teg ate gee gaa etc tta gaa atg cca 1592 jlu Asp Gly Val Asp Leu Ser Ser lie A丄a Glu Leu Leu Glu Met Pro 340 345 350 aac tta ttt att gat aat agt ate caa tta gaa aag aaa aag ttg tet 1640

Asn Leu Phe lie Asp Asn Ser He Gin Leu Glu Lys Lys Lys Leu Ser 355 360 365 cct att gag gat eta gat gaa gaa cca cgt aaa aat ctg gat ata gaa 1688

Pro lie Glu Asp Leu Asp Glu Glu Pro Arg Lys Asn Leu Asp lie Glu 370 375 380 385

gaa age cat tet aat tea teg gat gac gtg ett age atg aat gat gat 1736

Glu Ser His Ser Asn Ser Ser Asp Asp Val Leu Ser Met Asn Asp Λβρ 390 395 400 gag tet gat aca gac gat age aag tgg agt atg ggc aat gat 9在9 aaa 1784

Glu Ser Asp Thr Asp Asp Ser Lys Trp Ser Met Gly Asn Asp Glu Lys 405 410 415 gag atg ccc gat gat aag ctg ggt ata agt cgt gat gat cgt gga aat 1832

Glu Met Pro Asp Asp Lys Leu Gly lie Ser Arg Asp Asp Arg Gly Asn 420 425 430 aaa cca cct cgt act gat cct aca get gat tat ett aac cct gat gaa 1880

Lys Pro Pro Arg Thr Asp Pro Thr Val Asp Tyr Leu Asn Pro Asp Glu 435 440 445 ttc ttt gaa aat ggt tat etc ttg aat gag eta eta cag gag ett gga. 1928

Phe Phe Glu Asn Gly Tyr Leu Leu Asn Glu Leu Leu Gin Glu Leu Gly 450 455 460 465 gaa gag cog tta eta aaa gaa ggg gaa gat cat ttt aaa cgt tet acc 1976

Glu Glu Pro Leu Leu Lys Glu Gly Glu Asp His Phe Lys Arg Ser Thr

470 475 4β0 aat eta gtc cgt eta ggc gag aga gat agg caa aat aga gaa aag aga 2024

Asn Leu Val Arg Leu Gly Glu Arg Asp Arg Gin Asn Arg Glu Lys Arg 485 490 495 gaa aaa gag ggg tat ttt gat ctg cct ggt aca tta gat atg aaa ctg 2072

Glu Lys Glu Gly Tyr Phe Asp Leu Pro Gly Thr Leu Asp Met Lys Leu 500 505 510 cag gag tta ttc gaa aaa aga aaa caa aaa cac gaa gca gaa cag aaa 2120

Gin Glu Leu Phe Glu Lys Arg Lys Gin Lys His Glu Ala Glu Gin Lys 515 520 525 gca aga ata gaa aaa gca ett eta caa aaa tea gaa caa caa gaa aaa 2168

Ala Arg lie Glu Lys Ala Leu Leu Gin Lys Ser Glu Gin Gin Glu Lys 530 535 540 545 cgt gtt gaa gaa cgt aag caa gag gaa aaa cgt caa geg caa gat aaa 2216

Arg Val Glu Glu Arg Lys Gin Glu Glu Lys Arg Gin Ala Gin Asp Lys 550 555 560 -25· 136852·序歹!|表.doc 1334884

att get aag caa gta gaa att gca aaa gaa atg caa egg gta gaa gaa 2264 lie Ala Lys Gin 565 Val Glu lie Ala Lys 570 Glu Met Gin Arg Val 575 Glu Glu att ege cag aga gaa aaa caa ett geg ate caa ctg caa gaa gaa gag 2312 lie Arg Gin 580 Arg Glu Lys Gin Leu 585 Ala lie Gin Leu Gin 590 Glu Glu Glu aag aaa caa caa gaa gaa aaa cat tta tee gag gag aaa aaa caa get 2360 Lys Lys 595 Gin Gin Glu Glu Lys 600 His Leu Ser Glu Glu 605 Lys Lys Gin Ala gaa cag aaa caa aaa get gag gag aaa gtt gca caa gaa aga tta gac 2408 Glu 610 Gin Lys Gin Lys Ala 615 Glu Glu Lys Val Ala 620 Gin Glu Arg Leu Asp 625 att gaa caa cag aaa geg tat gaa gaa atg geg aag ega gag gca gag 2456 lie Glu Gin Gin Lys 630 Ala Tyr Glu Glu Met 635 Ala Lys Arg Glu Ala 640 Glu gca tea aaa aat gtt tta ttg aaa geg att 9at gaa gaa cgt cca aaa 2504 Ala Ser Lys Asn 645 Val Leu Leu Lye Ala 650 lie Asp Glu Glu Arg 655 Pro Lys gtg gaa act gat cca ett ttc cgt aca aaa ttg aaa tat ate aat caa 2552 Val Glu Thr Asp 660 Pro Leu Phe Arg 665 Thr Lys Leu Lys Tyr 670 lie Asn Gin gat gac tat get ggt gca aat tat ttc ttc aat aaa gtt ggt tta aat 2600 Asp Asp 675 Tyr Ala Gly Ala Asn 680 Tyr Phe Phe Asn Lys 685 Val Gly Leu Λ3Ζ1 aca aaa ggt cat caa aaa gta aat gtg tta ggg gat aac tat ttt gat 2648 Thr 690 Lys Gly His Gin Lys 695 Val Asn Val Leu Gly Asp Asn Tyr 700 Phe Asp 70S cat caa gtg att act ege teg att gag aaa aaa gta gat aac cac ett 2696 His Gin Val lie Thr 710 Arg Ser lie Glu Lys 715 Lys Val A.sp Asn His 720 Leu aac caa aaa tac aat etc age gat gtg gaa tta gtt aaa cag ctg atg 2744 Asn Gin Lys Tyr 725 Asn Leu Ser Asp Val 730 Glu Leu Val Lys Gin 735 Leu Met gac aat tee aca aca caa geg cag gag ttg gat ttg aaa eta ggt geg 27 92 Asp Asn Ser 740 Thr Thr Gin Ala Gin 745 Glu Leu Asp Leu Lys 750 Leu Gly Ala gca tta act aaa gaa caa caa get aac ttg acc caa gat ate gtt tgg 2840 Ala Leu 755 Thr Lys Glu Gin Gin 760 Ala Asn Leu Thr Gin 765 Asp lie Val Trp tat gtc aaa aeg aag gta aag ggc aaa gat gtg ttt gtt cca aag gtt 2888 Tyr 770 Val Lys Thr Lys Val 775 Lys Gly Lys Asp Val 780 Phe Val Pro Lys Val 785 tat ttc get tet gaa aeg etc gta gaa gee caa aaa tta caa ggt tta 2936 Tyr Phe Ala Ser Glu 790 Thr Leu Val Glu Ala 795 Gin Lys Leu Gin Gly 800 Leu ggc act ggg act ate aga gtt ggt gaa get aag att aaa gee aaa gat 2964 Gly Thr Gly Thr 805 lie Arg Val Gly Glu 810 Ala Lys lie Lys Ala 815 Lys Asp 136852-序列表.doc -26- ιζι 1334884 gtg gtg aat acc ggg aca tta get ggg aga aaa etc aat gtt gaa gcg 3032

Val Val Asn Thr Gly Thr Leu Ala Gly Arg Lys Leu Asn Val Glu Ala 820 825 830 agt aat aaa ate aaa aat caa ggg agt ate tta agt act caa gaa aca 3080

Ser Asn Lys lie Lys Asn Gin Gly Ser lie Leu Ser Thr Gin Glu Thr 835 840 845 cgt tta gtc ggg cgt aaa ggt att gaa aac gta tet cgt tea ttt gca 3128

Arg Leu Val Gly Arg Lys Gly He Glu Asn Val Ser Arg Ser Phe Ala 850 855 860 Θ65 aat gat gaa tta gga gtc act gca caa ege tea gaa ate aaa aeg gaa 3176

Asn Asp Glu Leu Gly Val Thr Ala Gin Arg Ser Glu He Lys Thr Glu 870 875 880 ggt cat tta cat ett gaa aca gat aag gat tea act att gat gta caa 3224

Gly His Leu His Leu Glu Thr Asp Lys Asp Ser Thr He Asp Val Gin 885 890 895

gca teg gat att aaa gca aaa aca age ttt gtg aag act ggt gat gtg 3272

Ala Ser Asp lie Lys Ala Lys Thr Ser Phe Val Lys Thr Gly Asp Val 900 905 910 aat etc aaa aat aca tac aat act aaa cat gee tac cgt gag aaa ttc 3320

Asn Leu Lys Asn Thr Tyr Asn Thr Lys His Ala Tyr Arg Glu Lys Phe 915 920 925 teg ccg agt gca eta caa gtt gca gaa ett gat gtg gca ggg ett aaa 3368

Ser Pro Ser Ala Leu Gin Val Ala Glu Leu Asp Val Ala Gly Leu Lys 930 935 940 945 gtc cca ett tta ggc gtg teg tet cca tee agt tat tea gag cat act 3416

Val Pro Leu Leu Gly Val Ser Ser Pro Ser Ser Tyr Ser Glu His Thr 950 955 960 agt gag gca act tea gag gga tea ate ttc gaa gta ggg cac tta cat 3464

Ser Glu Ala Thr Ser Glu Gly Ser lie Phe Glu Val Gly His Leu His 965 970 975 ett geg gta gac aga gat gtg aac caa geg ggg agt aaa att aag get 3512

Leu Ala Val Asp Arg Asp Val Asn Gin Ala Gly Ser Lys lie Lys Ala 980 985 990 aag tat acc act ggt gtt gtg aaa ggg aac ttt aat act gaa geg ggc 3560

Lys Tyr Thr Thr Gly Val Val Lys Gly Asn Phe Asn Thr Glu Ala Gly 995 1000 1005 aag aat att aaa cat gtc gaa aaa gaa gaa tat agt tea cag eta ttt 3608

Lys Asn lie Lys His Val Glu Lys Glu Glu Tyr Ser Ser Gin Leu Phe 1010 1015 1020 1025 get tea gca cac geg agt ggt ggt ggc acc tea gtt cgt tat gac tat 3656

Ala Ser Ala His Ala Ser Gly Gly Gly Thr Ser Val Arg Tyr Asp Tyr 1030 1035 1040 aac age caa gat ggt ggc aat gee tet gtt ggt gtt ccg aca aac cat 3704

Asn Ser Gin Asp Gly Gly Asn Ala Ser Val Gly Val Pro Thr Asn His 1045 1050 1055 act gga gtt ggg gca gag gca gga atg tea ttc acc cat acc aaa gac 3752

Thr Gly Val Gly Ala Glu Ala Gly Met Ser Phe Thr His Thr Lys Asp 1060 1065 10*70 -27 136852·序列表.doc

1 SI

1334884 aaa gaa aca ctg ccc act cac acc aat agt gaa tta caa gtc aaa cat Lys Glu Thr Leu Leu Thr His Thr Asn Ser Glu Leu Gin Val Lys His 107b 1080 1085 ggg aaa tta cat gtg ctt ggt tat gcc gat att ggt gga gta gat att Gly Lys Leu His Val Leu Gly Tyr Ala Asp lie Gly Gly Val Asp lie 1090 X095 1100 1105 aat act aaa eta cca gaa gat gca caa age aaa gca cag aaa gag ata Asn Thr Lys Leu Pro Glu Asp Ala Gin Ser Lys Ala Gin Lys Glu lie 1110 1115 1120 geg get age aag cca gag aag acc gag caa tet gca cag gat gtg get Ala Ala Ser Lys Pro Glu Lys Thr Glu Gin Ser Ala Gin Asp Val Ala 112S 1130 1135 caa get caa tea aat gcc aat aag gat aag gaa aat aaa gcc cca gaa Gin Ala Gin Ser Asn Ala Asn Lys Asp Lys Glu Asn Lys Ala Pro Glu 1140 1145 1150 ata aaa gaa tta tea gag get gaa ate geg gat etc atg tea gaa aaa lie Lys Glu Leu Ser Glu Ala Glu lie Ala Λβρ Leu Met Ser Glu Lys 1155 1160 1165 tea aaa geg tat ttt gat gat ttt gca gag caa geg aaa aaa gca cct Ser Lys Ala Tyr Phe Asp Asp Phe Ala Glu Gin Ala Lys Lys Ala Pro 1X70 1175 1180 1185 gaa aac aat ega ttt gaa ttg tet geg aaa gaa att aag teg age aaa Glu Asn Asn Arg Phe Glu Leu Ser Ala Lys Glu lie Lys Ser Ser Lys 1190 1195 1200 caa aaa gac caa tat gat cat gag tet gaa egg aeg act ttt aaa gtt Gin Lys Asp Gin Tyr Asp His Glu Ser Glu Arg Thr Thr Phe Lys Val 1205 1210 1215 gga cct gaa geg gag get cat tet geg gtt gcc gat atg gtg age cat Gly Pro Glu Ala Glu Ala His Ser Ala Val Ala Asp Met Val Ser Hxs 1220 1225 1230 tta gtg aaa gaa tat aga gat gca caa aat ggg act aaa caa gac ggt Leu Val Lys Glu Tyr Arg Asp Ala Gin Asn Gly Thr Lys Gin Asp Gly 1235 1240 1245 aca gta gca tta caa cat get agt gat gtc tta aat att gtg aeg ggg Thr Val Ala Leu Gin His Ala Ser Asp Val Leu Asn lie Val Thr Gly 1250 1255 1260 1265 gat tta geg ggg agt tea get aaa ttg tet gtt gaa aga aca cat gag Asp Leu Ala Gly Ser Ser Ala Lys Leu Ser Val Glu Arg Thr His Glu 1270 1275 1280 aca aaa ega aeg aca gaa aeg ggg gat att gtt act aag att ggt ggc Thr Lys Arg Thr Thr Glu Thr Gly Asp lie Val Thr Lys lie Gly Gly 1285 1290 1295 aat gtc aca ctg tea gca ege agt ggt agt gtg aac ctt aaa aat gta Asn Val Thr Leu Ser Ala Arg Ser Gly Ser Val Asn Leu Lys Asn Val 1300 1305 1310 caa agt: gac gaa caa get aat ttg acc tta aga gca aaa gaa gat gtg Gin Ser Asp Glu Gin Ala Asn Leu Thr Leu Arg Ala Lys Glu Asp Val 1315 1320 1325 136852-序歹!1 表.doc •28-

3800 3848 3896 3944 3992 4040 4088 4136 41Θ4 4232 4280 4328 4376 4424 4472 4520 I SI 1334884

aat gtg ctg tet ggt gaa aaa aca ega gaa acc aca gaa aca gta tea 4568 Asn Val Leu Ser Gly Glu Lys Thr Arg Glu Thr Thr Glu Thr Val Ser 1330 1335 1340 1345 aga cag aaa ett tet cat ggt gtg aac gca ggt tgc agt atg atg agt 4616 Arg Gin Lys Leu Ser His Gly val Asn Ala Gly Cys Ser Met Met Ser 1350 1355 1360 ggc gee tgt act gee ggt gtt age aca tea ett gaa gga aat gaa age 4664 Gly Ala Cys Thr Ala Gly Val Ser Thr Ser Leu Glu Gly Asn Glu Ser 1365 1370 1375 tat aeg tea gaa cgt gaa aeg get caa aat aac agt ttc tta aaa gca 4712 Tyr Thr Ser Glu Arg Glu Thr Ala Gin Asn Asn Ser Phe Leu Lys Ala 1380 1385 1390 cgc aac atg aaa gtt gaa gca ggt cgc gat ttt aat gtt gtc agt teg 4760 Arg Asn Met Lys Val Glu Ala Gly Arg Asp Phe Asn Val val Ser Ser 1395 1400 1405 aat att gat gca gat aag etc gat etc cac gtt aaa gga aaa aeg aat 4808 Asn lie Asp Ala Asp Lys Leu Asp Leu His Val Lys Gly Lys Thr Asn 1410 1415 1420 1425 gtg gta tec aaa caa gat aeg tta caa aaa gtg aeg cat gga gtt gac 4856 Val Val Ser Lys Gin Asp Thr Leu Gin Lys Val Thr His Gly Val Aep 1430 1435 1440 tat aat ett tee get ggc gtt gca ett tet agt gca aca att get acc 4904 Tyr Asn Leu Ser Ala Gly Val Ala Leu Ser Ser Ala Thr lie Ala Thr 1445 1450 1455 cca acc ggt aat gtt ggt ttc ggt tat acc aat gag acc gaa age aag 4952 Pro Thr Gly Asn Val Gly Phe Gly Tyr Thr Asn Glu Thr Glu Ser Lys 1460 1465 1470 egg aeg gtt aat caa caa gca ggg att aaa geg aat aaa att aca 999 5000 Arg Thr Val Asn Gin Gin Ala Gly lie Lys Ala Asn Lys lie Thr Gly 1475 1480 1485 caa aeg cat gac tta aat ett gag ggg gga tat ett gtc age aac gat 504Θ Gin Thr His Asp Leu Asn Leu Glu Gly Gly Tyr Leu Val Ser Asn Asp 1490 1495 1500 1505 aag gat aat cag ctg aaa gtt acc ggc gat gtc aca act aaa gee ett 5096 Lys Asp Asn Gin Leu Lys Val Thr Gly Asp Val Thr Thr Lys Ala Leu 1510 1515 1520 cac gat caa cat gat aaa gat ggt gga aca ttt ggt Cta tet gtg ggt 5144 His Asp Gin His Asp Lys Asp Gly Gly Thr Phe Gly Leu Ser Val Gly 1525 1530 1535 ate agt gaa cgt ggt act acc gee ttt aat gta ega 99t ggg ega get 5192 lie Ser Glu Arg Gly Thr Thr Ala Phe Asn Val Arg Gly Gly Arg Ala 1540 1545 1550 gaa cag aaa cac tat aat gca aeg cag aaa tee act ett tet ggc gtg 5240 Glu Gin Lys His Tyr Asn Ala Thr Gin Lys Ser Thr Leu Ser Gly Val 1555 1560 1565 gat acc tet caa geg aat gta tea ggt caa gtg aat aca gac tta acc 5288 Asp Thr Ser Gin Ala Asn Val Ser Gly Gin Val Asn Thr Asp Leu Thr 1570 1575 1580 1585 136852-序列表.doc -29-

ί SI 1334884

aag gca aaa get gtc aca cgt gat gat act tac gca agt aeg caa ttt Lys Ala Lys Ala Val Thr Arg Asp Asp Thr Tyr Ala Ser Thr Gin Phe 1590 1595 1600 agt ttt gaa gtg gca gat att gtg gaa tta gga cag aga geg aaa aac Ser Phe Glu Val Ala Asp lie Val Glu Leu Gly Gin Arg Ala Lys Asn 1605 1610 1615 aag ctg tea gca cca aac aat gac acc gat atg geg tea ggc tee aca Lys Leu Ser Ala Pro Asn Asn Asp Thr Asp Met Ala Ser Gly Ser Thr 1620 1625 1630 tta ege teg cgt tet act aca gaa gaa gca gat gta cca aca aca aga Leu Arg Ser Arg Ser Thr Thr Glu Glu Ala Asp Val Pro Thr Thr Arg 1635 1640 1645 teg cgt gta aeg gat gag geg gat tet gta tee gtg aaa aat ccg att Ser Arg Val Thr Asp Glu Ala Asp Ser Val Ser Val Lys Asn Pro lie 1650 1655 1660 1665 tat gaa agt gca gat get gtt gta cca aca cca cgt agt aga aat gtg Tyr Glu Ser Ala Asp Ala Val Val Pro Thr Pro Arg Ser Arg Asn Val 1670 1675 1680 gac agt acc gat ett gtg gac aat cca ctg tat get agt gee act aca Asp Ser Thr Asp Leu Val Asp Asn Pro Leu Tyr Ala Ser Ala Thr Thr 1685 1690 1695 aaa gca aac ate cat gat tat gaa gaa att cca gee gtt tat age aaa Lys Ala Asn lie His Asp Tyr Glu Glu lie Pro Ala Val Tyr Ser Lys 1700 1705 1710 gtc ggt gat aac aat get gat ett gtt cgt cat aaa aeg gca act agt Val Gly Asp Asn Asn Ala Asp Leu Val Arg His Eys Thr Ala Thr Ser 1715 1720 1725 gat gag cat ett tat gca gag att aat gaa cca aca tat age cgt gtt Asp Glu His Leu Tyr Ala Glu lie Asn Glu Pro Thr Tyr Ser Arg Val 1730 1735 1740 1745 ggt gat aaa aat gca gat atg aga cgt cat aac geg gca ggt aca aca Gly Asp Lys Asn Ala Asp Met Arg Arg His Asn Ala Ala Gly Thr Thr 1750 1755 1760 gac tat gee gat gtc gtg caa gca cat aca aga aag gca gat gat cca Asp Tyr Ala Asp Val Val Gin Ala His Thr Arg Lys Ala Asp Asp Pro 1765 1770 1775 eta cca gca tta ccg aat cag ggt aaa gca aga aeg gta aac gac ggt Leu Pro Ala Leu Pro Asn Gin Gly Lys Ala Arg Thr Val Asn Asp Gly 1780 1785 1790 tea gag cat att tat act gat att age gac gtg ggc act caa act aaa Ser Glu His lie Tyr Thr Asp He Ser Asp Val Gly Thr Gin Thr Lys 1795 1800 1805 get att gat agt act tat gca aca gta ggc atg ccg aaa geg aat gee Ala lie Asp Ser Thr Tyr Ala Thr Val Gly Met Pro Lys Ala Asn Ala 1810 1815 1820 1825 gtt aac ttg ata ggg caa aat ggc tta ggc age att tat cac age cca Val Asn Leu lie Gly Gin Asn Gly Leu Gly Ser He Tyr His Ser Pro 1830 1835 1840 5336 5384 5432 5480 5528 5576 5624 5672 5720 5768 5816 5864 5912 5960 6008 6056 136852-序列表、doc -30· l Si 1334884

gac agt get tat aaa aca tgg caa ttg ett gat caa ttt gee aac aaa Asp Ser Ala Tyr Lys Thr Trp Gin Leu Leu Asp Gin Phe Ala Asn Lys 1845 1850 1855 ggc gga gat geg gtc ttc tta ege cct gca aca gaa atg aaa tgt gca Gly Gly Asp Ala Val Phe Leu Arg Pro Ala Thr Glu Met Lys Cys Ala I860 1865 1870 ggt gca cct tta aaa tat.acc ttt ate gtg cgt gat tat ttg etc aga Gly Ala Pro Leu Lys Tyr Thr Phe lie Val Arg Asp Tyr Leu Leu Arg 1875 ΧΘ80 1885 ege cat acc tta gat aaa tea aga tta ttt tat aac gca cat aat aaa Arg His Thr Leu Asp Lys Ser Arg Leu Phe Tyr Asn Ala His Asn Lys 1890 1895 1900 1905 acc tta ttt age gtg cct ate gtt gat gca aaa gtc aaa atg ctg ttt Thr Leu Phe Ser Val Pro lie Val Asp Ala Lys Val Lys Met Leu Phe 1910 1915 1920 get gaa aaa aat ate caa gtc aat tac gat cgt age ett aca gee att Ala Glu Lys Asn lie Gin Val Asn Tyr Asp Arg Ser Leu Thr Ala lie 1925 1930 1935 gat ctg agt aaa cgt att geg acc ttt aat age cca gaa gga gtt gta Asp Leu Ser Lys Arg lie Ala Thr Phe Asn Ser Pro Glu Gly Val Val 1940 1945 1950 gaa gtc cct tat gat ttt att aat gtg gta cct cca atg ega gca cct Glu Val Pro Tyr Asp Phe lie Asn Val Val Pro Pro Met Arg Ala Pro 1955 I960 1965 gat gee gtt cgt caa tea gca etc geg tgg caa gaa gga aaa tgg get Asp Ala Val Arg Gin Ser Ala Leu Ala Trp Gin Glu Gly Lys Trp Ala 1970 1975 1980 1985 aac gat ggt tgg gtt gaa gta gaa aaa cat acc ttg cgt cac cgt cgt Asn Asp Gly Trp Val Glu Val Glu Lys His Thr Leu Arg His Arg Arg 1990 1995 2000 tat gee aat gtg ttt get gtg ggt gat gtg gca ggg gtc cca aaa ggc Tyr Ala Asn Val Phe Ala Val Gly Asp Val Ala Gly Val Pro Lys Gly 2005 2010 2015 aaa aeg get gee agt gtc aaa tgg caa gtt cct gtt gca gtg gca cat Lys Thr Ala Ala Ser Val Lys Trp Gin Val Pro Val Ala Val Ala His 2020 2025 2030 tta etc gca gaa tta gag ggc aaa cct tgt gat gaa att tac aac ggt Leu Leu Ala Glu Leu Glu Gly Lys Pro Cys Asp Glu He Tyr Asn Gly 2035 2040 2045 tat aca tet tgt cca tta att act caa tta gga aag ggg atg eta gca Tyr Thr Ser Cys Pro Leu lie Thr Gin Leu Gly Lys Gly Met Leu Val 2050 2055 2060 2065 gaa ttt gat tat aac aac cac tta aca cct tet ttc ccc ggt gta ata Glu Phe Asp Tyr Asn Asn His Leu Thr Pro Ser Phe Pro Gly Val lie 2070 2075 2080 geg cca tta gaa gaa ctg tgg gca aca tgg gca att aaa aca tta ggt Ala Pro Leu Glu Glu Leu Trp Ala Thr Trp Ala He Lys Thr Leu Gly 2085 2090 2095 6104 6152 6200 624β 6296 6344 6392 6440 6488 6536 6584 6632 6680 6728 6776 6824 136852-序歹!I表.doc -31 - u] 1334884 tta aaa ccc act tat tta ggt atg tta cgt gga tta get taaggagcgt Leu Lys Pro Thr Tvr Leu Glv Met Leu Arq Gly Jjeu Ala 2100 2105 2110 tga 6873 6876

<210> 1β <211> 2110 <212>蛋白質 <213>敗血性巴斯德样菌 <400> 16 Met Gin Pro Ala Gin Glu His Cys Gin Arg He Asn Asn lie Val Aen 15 10 15 Gin Glu Asn Gly Leu Phe His Thr Leu Gly Asn Met Met Leu Glu Ala 20 25 30 Glu Arg Ser Val Tyr Asn lie Gly Asp He Tyr Ala Ser Lys Lys Leu 35 40 45 Thr Val His Thr His Asn Leu lie Asn Asp Val Arg Leu Ser Gly Asn 50 55 60 Val Ser Tyr Lys Pro lie Gly Ser Ser Arg Asp Tyr Asp lie Ser Arg 65 70 75 80 Val Ala Val His Gly Trp His Asn Asn Val Tyr Lys Leu Asn Leu Asn 85 90 95 Leu Gin Glu Gin Asp Lys Thr Asp lie Lys Val Val Lys Met Gly Ala 100 105 110 lie Arg Ser Asp Gly Asp Phe Asp Phe Lys Gly lie Lys Ala Thr Ser 115 120 125 Ser Glu Ser Lys Pro Gin Leu He Asn His Gly Leu lie Asn Val Lys 130 135 140 Gly Thr Phe Asn Ala Glu Ala Asp Gin Val Val Asn Gin Met Lys Ala 145 150 155 160 Phe Asn Gin Asn Ala Leu Ala Ser Val Phe Lys Asn Pro Ala Lys lie 165 170 175 Thr Met Tyr Tyr Gin Pro Leu Thr Arg Tyr lie Trp Thr Pro Leu Ser 100 185 190 Gly Asn Ala Ser Arg Glu Phe Asn Asn Leu Glu Ser Phe Leu Asp Ala 195 200 205 Leu Phe Gly Ser Thr Thr lie Leu Lys Ser Ser Phe Tyr Ser Thr Glu 210 215 220 Asn Phe Ser Ala Tyr Gin Leu Leu Ser His lie Gin His Ser Pro Met 225 230 235 240 Tyr Gin Lys Ala Met Ala Gin Val Phe Gly Ala Glu Trp His Ser Lys 245 250 255 Ser Tyr Asp Glu Met Arg Asn Lys Trp Lys Ser Phe Lys Glu Asn Pro 260 265 ' 270 136852-序列表.doc -32- 131

Thr Asp Phe lie Tyr Tyr Pro Ser Glu Lys Ala Lys lie Leu Ala Gly 275 280 285

Lys Leu Glu Gly Lys Leu Thr Thr Leu Gin Asn Gly Glu Tyr Ala Glu 290 295 300

Arg Gly Lys Phe Asp Glu Ser lie Gin lie Gly Lys His Gin Leu Ser 305 310 315 320

Leu Pro Ser Val Glu Leu Lys Ala Glu Pne Ser Asp Lys Glu Arg Leu 325 330 335

Glu Glu Asp Gly Val Asp Leu Ser Ser lie Ala Glu Leu Leu Glu Met 340 345 350

Pro Asn Leu Phe He Asp Asn Ser lie Gin Leu Glu Lys Lys Lys Leu 355 360 365

Ser Pro lie Glu Asp Leu Asp Glu Glu Pro Arg Lys Asn Leu Asp lie 370 375 380

Glu Glu Ser His Ser Asn Ser Ser Asp Asp Val Leu Ser Met Αβη Λβρ 385 390 395 400

Asp Glu Ser Αερ Thr Asp Asp Ser Lys Trp Ser Met Gly Asn Asp Glu 405 410 415

Lys Glu Met Pro Asp Λβρ Lys Leu Gly lie Ser Arg Asp Asp Arg Gly 420 425 430

Asn Lys Pro Pro Arg Thr Asp Pro Thr Val Asp Tyr Leu Asn Pro Asp 435 440 445

Glu Phe Phe Glu Asn Gly Tyr Leu Leu Asn Glu Leu Leu Gin Glu Leu 450 455 460

Gly Glu Glu Pro Leu Leu Lys Glu Gly Glu Asp His Phe Lys Arg Ser 465 470 475 480

Thr Asn Leu Val Arg Leu Gly Glu Arg Asp Arg Gin Asn Arg Glu Lys 485 490 495

Arg Glu Lys Glu Gly Tyr Phe Asp Leu Pro Gly Thr Leu Asp Met Lys 500 505 510

Leu Gin Glu Leu Phe Glu Lys Arg Lys Gin Lys His Glu Ala Glu Gin 515 520 525

Lys Ala Arg lie Glu Lys Ala Leu Leu Gin Lys Ser Glu Gin Gin Glu 530 535 540

Lys Arg Val Glu Glu Arg Lys Gin Glu Glu Lys Arg Gin Ala Gin Asp 545 550 555 560

Lys lie Ala Lys Gin Val Glu lie Ala Lys Glu Met Gin Arg Val Glu 565 570 575

Glu lie Arg Gin Arg Glu Lys Gin Leu Ala lie Gin Leu Gin Glu Glu 580 585 590

Glu Lys Lys Gin Gin Glu Glu Lys His Leu Ser Glu Glu Lys Lys Gin 595 600 605 •33· U6852·序列表.doc 1Ϊ3 1334884

Ala Glu Gin Lys Gin Lys Ala Glu Glu Lys Val Ala Gin Glu Arg Leu 610 615 620

Asp lie Glu Gin Gin Lys Ala Tyr Glu Glu Met Ala Lys Arg Glu Ala 625 630 635 640

Glu Ala Ser Lys Asn Val Leu Leu Lys Ala lie Asp Glu Glu Arg Pro 645 650 655

Lys Val Glu Thr Asp Pro Leu phe Arg Thr L> s Leu Lys Tyr lie Asn 660 665 670

Gin Asp Asp Tyr Ala Gly Ala Asn Tyr Phe Phe Asn Lys Val Gly Leu 675 680 685

Asn Thr Lys Gly His Gin Lys Val Asn Val Leu Gly Asp Asn Tyr Phe 690 695 700

Asp His Gin Val lie Thr Arg Ser lie Glu Lys Lys Val Asp Asn His 705 710 715 720

Leu Asn Gin Lys Tyr Asn Leu Ser Asp Val Glu Leu Val Lys Gin Leu 725 730 735

Met Asp Asn Ser Thr Thr Gin Ala Gin Glu Leu Asp Leu Lys Leu Gly 740 745 750

Ala Ala Leu Thr Lys Glu Gin Gin Ala Asn Leu Thr Gin Asp lie Val 755 760 765

Trp Tyr Val Lys Thr Lys Val Lys Gly Lys Asp Val Phe Val Pro Lys 770 775 780

Val Tyr Phe Ala Ser Glu Thr Leu Val Glu Ala Gin Lys Leu Gin Gly 7Θ5 790 795 800

Leu Gly Thr Gly Thr He Arg Val Gly Glu Ala Lys He Lys Ala Lys 805 810 815

Asp Val Val Asn Thr Gly Thr Leu Ala Gly Arg Lys Leu Asn Val Glu 820 825 830

Ala Ser Asn Jbys lie Lys Asn Gin Gly Ser lie Leu Ser Thr Gin Glu 835 840 845

Thr Arg Leu Val Gly Arg Lys Gly lie Glu Asn Val Ser Arg Ser Phe 850 855 860

Ala Asn Asp Glu Leu Gly Val Thr Ala Gin Arg Ser Glu lie Lys Thr 865 870 875 880

Glu Gly His Leu His Leu Glu Thr Asp Lys Asp Ser Thr lie Asp Val 885 890 895

Gin Ala Ser Asp lie Lys Ala Lys Thr Ser Phe Val Lys Thr Gly Asp 900 905 910

Val Asn Leu Lys Asn Thr Tyr Asn Thr Lys His Ala Tyr Arg Glu Lys 915 920 925

Phe Ser Pro Ser Ala Leu Gin Val Ala Glu Leu Asp Val Ala Gly Leu 930 935 940 136852·序列表.doc -34·

l SI 1334884

Lys Val Pro Leu Leu Gly Val Ser Ser Pro Ser Ser Tyr Ser Glu His 945 950 955 960

Thr Ser Glu Ala Thr Ser Glu Gly Ser lie Phe Giu Val Gly His Leu 965 970 975

His Leu Ala Val Asp Arg Asp Val Asn Gin Ala Gly Ser Lys lie Lys 980 985 990

Ala Lys Tyr Thr Thr Gly Val Val Lys Gly Asn Phe Asn Thr Glu Ala 995 1000 1005

Gly Lys Asn lie Lys His Val Glu Lys Glu Glu Tyr Ser Ser Gin Leu 1010 1015 1020

Phe Ala Ser Ala His Ala Ser Gly Gly Gly Thr Ser Val Arg Tyr Asp 1025 1030 1035 1040

Tyr Asn Ser Gin Asp Gly Gly Asn Ala Ser Val Gly Val Pro Thr Asn

1045 1050 1055

His Thr Gly Val Gly Ala Glu Ala Gly Met Ser Phe Thr His Thr Lys 1060 1065 1070

Asp Lys Glu Thr Leu Leu Thr His Thr Asn Ser Glu Leu Gin Val Lys 1075 1080 1085

His Gly Lys Leu His Val Leu Gly Tyr Ala Asp He Gly Gly Val Asp 1090 1095 1100 lie Asn Thr Lys Leu Pro Glu Asp Ala Gin Ser Lys Ala Gin Lys Glu 1105 1110 1115 1120 lie Ala Ala Ser Lys Pro Glu Lys Thr Glu Gin Ser Ala' Gin Asp Val 1125 1130 1135

Ala Gin Ala Gin Ser Asn Ala Asn Lys Asp Lys Glu Asn Lys Ala Pro 1140 1145 1150

Glu lie Lys Glu Leu Ser Glu Ala Glu lie Ala Asp Leu Net Ser Glu 1155 1160 1X65

Lys Ser Lys Ala Tyr Phe Asp Asp Phe Ala Glu Gin Ala Lys Lys Ala 1170 1175 1180

Pro Glu Asn Asn Arg Phe Glu Leu Ser Ala Lys Glu He Lys Ser Ser 1185 1190 1195 1200

Lys Gin bys Asp Gin Tyr Asp His Glu Ser Glu Arg Thr Thr Phe Lys 1205 1210 1215

Val Gly Pro Glu Ala Glu Ala His Ser Ala Val Ala Asp Met Val Ser 1220 1225 1230

His Leu Val Lys Glu Tyr Arg Asp Ala Gin Asn Gly Thr Lys Gin Asp 1235 1240 1245

Gly Thr Val Ala Leu Gin His Ala Ser Asp Val Leu Asn lie Val Thr 1250 1255 1260

Gly Asp Leu Ala Gly Ser Ser Ala Lys Leu Ser Val Glu Arg Thr His 1265 1270 1275 1280 •35· 136852-序歹 1J 表.doc i si 1334884 CUu Ttir Lys Arg Thr Thr Glu Thr Gly Asp He Val Thr Lys lie Gly 1285 1290 1295

Gly Asn Val Thr Leu Ser Ala Arg Ser Gly Ser Val Asn Leu Lys Asn 1300 1305 1310 Val Gin Ser Asp Glu Gin Ala Asn Leu Thr Leu Arg Ala Lys Glu Asp 1315 1320 1325 Val Asn Val Leu Ser Gly Glu Lys Thr Arg Glu Thr Thr Glu Thr Val 1330 1335 1340 Ser Arg Gin Lys Leu Ser His Gly Val Asn Ala Gly Cys Ser Met Met 1345 1350 1355 1360 Ser Gly Ala Cys Thr Ala Gly Val Ser Thr Ser Leu Glu Gly Asn Glu 1365 1370 1375 Ser Tyr Thr Ser Glu Arg Glu Thr Ala Gin Asn Asn Ser Phe Leu Lys 1380 1385 1390 Ala Arg Asn Met Lys Val Glu Ala Gly Arg Asp Phe Asn Val Val Ser 1395 1400 1405 Ser Asn lie Asp Ala Asp Lys Leu Asp Leu His Val Lys Gly Lys Thr 1410 1415 1420 Asn Val Val Ser Lys Gin Asp Thr Leu Gin Lys Val Thr His Gly Val 1425 1430 1435 1440 Asp Tyr Asn Leu Ser Ala Gly Val Ala Leu Ser Ser Ala Thr lie Ala 1445 1450 1455 Thr Pro Thr Gly Asn Val Gly Phe Gly Tyr Thr Asn Glu Thr Glu Ser 1460 1465 1470 Lys Arg Thr Val Asn Gin Gin Ala Gly He Lys Ala Asn Lys lie Thr

1475 1480 1485 Gly Gin Thr His Asp Leu Asn Leu Glu Gly Gly Tyr Leu Val Ser Asn 1490 1495 1500 Asp Lys Asp Asn Gin Leu Lys Val Thr Gly Asp Val Thr Thr Lys Ala 1505 1510 :515 1520 Leu His Asp Gin His Asp Lys Asp Gly Gly Thr Phe Gly Leu Ser Val 1525 1530 1535

Gly lie Ser Glu Arg Gly Thr Thr Ala Phe Asn Val Arg Gly Gly Arg 1540 1545 1550 Ala Glu Gin Lys His Tyr Asn Ala Thr Gin lys Ser Thr Leu Ser Gly 1555 1560 " 1565 Val Asp Thr Ser Gin Ala Asn Val Ser Gly 3ln Val Asn Thr Asp Leu 1570 1575 1580 Thr Lys Ala Lys Ala Val Thr Arg Asp Asp Thr Tyr Ala Ser Thr Gin 1585 1590 1595 1600

Phe Ser Phe Glu Val Ala Asp lie Val Glu leu Gly Gin Arg Ala Lys 1605 1610 1615 136852-序列表.doc •36· tsi 1334884

Asn Lys Leu Ser Ala Pro Asn Asn Asp Thr Asp Met Ala Ser Gly Ser 1620 1625 1630

Thr Leu Arg Ser Arg Ser Thr Thr Glu Glu Ala Asp Val Pro Thr Thr 1635 1640 1645

Arg Ser Arg Val Thr Asp Glu Ala Asp Ser Val Ser Val Lys Asn Pro 1650 1655 1660 lie Tyr Glu Ser Ala Asp Ala Val Val Pro Thr Pro Arg Ser Arg Asn 1665 1670 1675 1680

Val Asp Ser Thr Asp Leu Val Asp Asn Pro Leu Tyr Ala Ser Ala Thr 1685 1690 1695

Thr Lys Ala Asn lie His Asp Tyr Glu Glu lie Pro Ala Val Tyr Ser 1700 1705 1710

Lys Val Gly Asp Asn Asn Ala Asp Leu Val Arg His Lys Thr Ala Thr 1715 1720 1725

Ser Asp Glu His Leu Tyr Ala Glu lie Asn Glu Pro Thr Tyr Ser Arg 1730 1735 1740

Val Gly Asp Lys Asn Ala Asp Met Arg Arg His Asn Ala Ala Gly Thr 1745 1750 1755 1760

Thr Asp Tyr Ala Asp Val Val Gin Ala His Thr Arg Lys Ala Asp Asp 1765 1770 1775

Pro Leu Pro Ala Leu Pro Asn Gin Gly Lys Ala Arg Thr Val Asn Asp 1780 1785 1790

Gly Ser Glu His lie Tyr Thr Asp lie Ser Asp Val Gly Thr Gin Thr 1795 1800 1805

Lys Ala lie Asp Ser Thr Tyr Ala Thr Val Gly Met Pro Lys Ala Asn 1810 1815 1820

Ala Val Asn Leu lie Gly Gin Asn Gly Leu Gly Ser lie Tyr His Ser 1825 1830 X835 1840

Pro Asp Ser Ala Tyr Lys Thr Trp Gin Leu Leu Asp Gin Phe Ala Asn 1845 1850 1855

Lys Gly Gly Asp Ala Val Phe Leu Arg Pro Ala Thr Glu Met Lys Cys 1860 1865 1870

Ala Gly Ala Pro Leu Lys Tyr Thr Phe lie Val Arg Asp Tyr Leu Leu 1875 1880 1885

Arg Arg His Thr Leu Asp Lys Ser Arg Leu Phe Tyr Asn Ala His Asn 1890 1895 1900

Lys Thr Leu Phe Ser Val Pro He Val Asp Ala Lys Val Lys Met Leu 1905 1910 1915 1920

Phe Ala Glu Lys Asn lie Gin Val Asn Tyr Asp Arg Ser Leu Thr Ala 1925 1930 1935 lie Asp Leu Ser Lys Arg He Ala Thr Phe Asn Ser Pro Glu Gly Val 1940 1945 1950 -37- 136852·序列表.doc

1334884

Val Glu Val Pro Tyr Asp Phe lie Asn Val Val Pro Pro Met Arg Ala \9SS 1^60 1965

Pro Asp Ala Val Arg Gin Ser Ala Leu Ala Trp Gin Glu Gly r»ys Trp 1970 1975 I960

Ala Asn Asp Gly Trp Val Glu Val Glu Lys His Thr Leu Arg His Arg 1985 1990 1995 2000

Arg Tyr Ala Asn Val Phe Ala Val Gly Asp Val Ala Gly Val Pro hys 2005 2010 201S

Gly Lys Thr Ala Ala Ser Val Lys Trp Gin Val Pro Val Ala Val Ala 2020 2025 2030

His Leu Leu Ala Glu Leu Glu Gly Lys Pro Cys Asp Glu He Tyr Asn 2035 2040 2045

Gly Tyr Thr Ser Cys Pro Leu lie Thr Gin Leu Gly Lys Gly Met Leu 2050 2055 2060

Val Glu Phe Asp Tyr Asn Asn His Leu Thr Pro Ser Phe Pro Gly Val 2065 2070 2075 2080 lie Ala Pro Leu Glu Glu Leu Trp Ala Thr Trp Ala lie Lys Thr Leu 2065 2090 2095

Gly Leu Lys Pro Thr Tyr Leu Gly Met Leu Arg Gly Leu Ala 2100 2105 2110

<210> 17 <211> 3247 <212> DNA <213>敗血性巴斯德样茵 <220>

<221> CDS <222> (1479) .. (3245) <220>

<223> £haB2 <400> 17 gtcgaccttg cgggtgaaaa ggtatctcta aattttggcg atatcattca tgcttaccaa 60 aaccagcccc tatcaacaaa agttgttttt caattagtga aagatttgac ggaagtttta 120 taccgttctg gctacgtgac aagtgcaatt ggtttaaaaa attcaaaaat cagcaatggc 180 gatcttgaat ttattgtact gtggggaaga actcgcgatc tgtttgtgaa tggggagaaa 240 ccaacccgtt ttagagataa aacaatgtta tcagtcctac ccaatttaat cggaaatcgc 300 ttaagtattc acgacattga ccagttgatc gaaatcttaa atactacgaa taaaaaagcc 360 acagtgaatg tggttgcaag tgaagaaaaa ggcagctcaa atctaaatat tgaaagacaa 420 tatgatgttt ttccgcaagt gagtgtcgga ttcaataatt caggtgctgg caataatgcc 480 aatgggcgta atcaagctac attgaatatt gcttggagtg atctattagg cacgaatgat 540 cqttqqaqtt tctcqaqtaq ttaccgttta tataaaaatc atcat.gctaa ccaacaacqc 600 •38· 136852-序列表.doc

LSI 1334884 aattatactt tgtcttacag tcagcctata ggcttttcta cagtagaaat taaagcatcg 660 gaatctacgt atgaaaaaga acttcgcggt acaaatactc attcttctca tgggaaaacc 720 caaagcttag ctgtcaagct gatgcatgtg ttattgcgta ataaggagag tattttatct 780 acatataccg agttcgagtt taaaaaacgg attagttatt tttctgatat tttgattggg 840 aaatatcaca ataataaagt gagcgtaggg ttatcttaca tgactaattt tgcttacggg 900 aagctttaca gcgacattgc ttacgcgaat gggttgagat ggtttggggc gaattattca 960 gcatatgatg caaatcgtga aaaaacctta aaattattgt caggaagtat taattggcag 1020 cgtccaatat ccctgtttga acgtgcgatg aattatcaat tacgtattgg tgcccaatat Χ0Θ0 ggttttgata gtttgtattc tgaaaatcaa ttttcaattg gtgatgaata tacagtaaga 1140 ggatttaaag gtggtgcggt ttctggtgat agtggtgcgt atttatcaca aacactgacg 1200

gttccttttt atccacaaaa agcatattta tctcaggtat ccccttttat tggatttgat 1260 atgggtaaag tacatattaa atcaaagcat aaaacaacca ctttagtcgg ttttgcccta 1320 ggcttgaaaa cgcaaataaa gttattttca ttatcattaa cctatgcaca accaatgaat 1380 ggtgtgagtg gtgttacgca acatcgtcaa aaaccgattt attatttctc aggatcactt 1440 tctttttaat ctcttttaag tttaaggatt aacttaat atg aac aaa aat cgt tac 1496

Met Asn Lys Asn Arg Tyr 1 5 aaa etc att ttt agt caa gtc aaa ggt tgt etc gtt cct gtg gca gaa 1544

Lys Leu lie Phe Ser Gin Val Lys Gly Cys Leu Val Pro Val Ala Glu 10 15 20 tgt att aac tea get att age aat ggt tea tet gat tea aca tee aca 1592

Cys lie Asn Ser Ala lie Ser Asn Gly Ser Ser Asp Ser Thr Ser Thr 25 30 35 tea gaa caa gtt gaa gag gaa cct ttc ett eta gaa caa tat tea ett 1640

Ser Glu Gin Val Glu Glu Glu Pro Phe Leu Leu Glu Gin Tyr Ser Leu

40 45 50 tee tee gtg tet: tta tta gta aaa age aeg ttc aat cct gtt teg tat 1688

Ser Ser Val Ser Leu Leu Val Lys Ser Thr Phe Asn Pro Val Ser Tyr 55 60 65 70 gca atg caa ttg act tgg aaa cag ett tet att tta ttt tta act gtg 1736

Ala Met Gin Leu Thr Trp Lys Gin Leu Ser lie Leu Phe Leu Thr Val 75 80 85 att tet gtt cct gtt ttg get gag gga aaa ggg gat gaa aga aat caa 1784 lie Ser Val Pro Val Leu Ala Glu Gly Lys Gly Asp Glu Arg Asn Gin . 90 95 100 tta aca gtg att gat aat age gat cat att aaa tta gat gca tet aat 1832

Leu Thr Val lie Asp Asn Ser Asp His lie Lys Leu Asp Ala Ser Asn 10S 110 115 ett get ggt aat gat aaa aca aaa ate tat caa gca gaa aat aaa gtt 1880

Leu Ala Gly Asn Asp Lys Thr Lys lie Tyr Gin Ala Glu Asn Lys Val 120 125 130 -39- 136852·序列表 _doc

i SI 1334884

ctg gtt att gat att get aaa cca aat ggg aaa ggg att tea gat aac Leu Val lie Asp lie Ala Lys Pro Asn Gly Lys Gly He Ser Asp Asn 135 140 145 150 cgt ttt gaa aaa ttt aat att cca aat age geg gtg ttt aat aat aat Arg Phe Glu Lys Phe Asn lie Pro Asn Ser Ala Val Phe Asn Asn Asn 155 160 165 ggg act gaa geg cag gca aga tea aca tta att ggt tac att ccg caa Gly Thr Glu Ala Gin Ala Arg ".er Thr Leu lie Gly Tyr lie Pro Gin 170 175 180 aat caa aat tta. agg gga ggg aaa gaa get gat gtt ata tta aat caa Asn Gin Asn Leu Arg Gly Gly Lys Glu Ala Asp Val lie Leu Asn Gin 185 190 195 gtg aca ggt cct caa gaa agt aaa att gtt ggc geg ett gaa gta tta Val Thr Gly Pro Gin Glu Ser Lys lie Val Gly Ala Leu Glu Val Leu 200 205 210 ggt aaa aaa get gat ate gtc att gca aac caa aat ggt att acc tta Gly Lys Lys Ala Asp lie Val lie Ala Asn Gin Asn Gly lie Thr Leu 215 220 225 230 aat ggt gta aga aca ata aat tea gat cgt ttt gtt gcc act aeg ogt Asn Gly Val Arg Thr lie Asn Ser Asp Arg Phe Val Ala Thr Thr Ser 235 240 245 gag ett ata gat ccg aat cag atg atg tta aag gtt aca aaa gga aat Glu Leu lie Asp Pro Asn Gin Met Met Leu Lys Val Thr Lys Gly Asn 250 255 260 gtg ate att gat att gat ggt ttt teg aca gat gga tta aag tat tta Val lie lie Asp lie Asp Gly Phe Ser Thr Asp Gly Leu Lys Tyr Leu 265 270 275 gat att att get aaa aaa att gaa caa aag caa tea att aca tea ggg： Asp lie lie Ala Lys Lys lie Glu Gin Lys Gin Ser lie Thr Ser Gly 280 285 290 gat aat tea gaa gca aaa aca gat gtc act ett att geg ggt tcc agt Asp Asn Ser Glu Ala Lys Thr Asp Val Thr Leu lie Ala Gly Ser Ser 295 300 305 310 gaa tat gat tta age aaa cat gag ctg aaa aaa aeg age ggt gaa aat Glu Tyr Asp Leu Ser Lys His Glu Leu Lys Lys Thr Ser Gly Glu Asn 315 320 325 gta tet aat gat gtt att get ate aeg gga tet agt aca ggc gca atg Val Ser Asn Asp Val lie Ala lie Thr Gly Ser Ser Thr Gly Ala Met 330 335 340 cat ggt aaa aat att aag ttg att gtg aca gat aaa ggt gca ggc gta His Gly Lys Asn lie Lys Leu lie Val Thr Asp Lys Gly Ala Gly Val 345 350 355 aaa cat gat gga att att ttg tet gaa aat gat att cag att gaa atg Lys His Asp Gly lie lie Leu Ser Glu Asn Asp lie Gin lie Glu Met 360 365 370 aat gaa ggt gac tta gaa ett ggc aat aeg att cag caa aca gtg gta Asn Glu Gly Asp Leu Glu Leu Gly Asn Thr lie Gin Gin Thr Val Val 375 380 385 390 1928 1976 2024 2072 2120 2168 2216 2264 2312 2360 2408 2456 2504 2552 2600 2648 136852·序列表.doc 40· 2696 aaa aaa gac cga aat att cga gcc aag aaa aaa att gaa gtg aaa aac

Lys Lys Asp Arg Asn lie Arg Ala Lys Lys Lys lie Glu Val Lys Asn 395 400 405 get aat cgt gtt ttt gtt ggt agt caa aeg aaa tea gat gaa att teg 2744

Ala Asn Arg Val Phe Val Gly Ser Gin Thr Lys Ser Asp Glu lie Ser 410 415 420 tta gag geg aaa caa gtt aaa ate aga aaa aac gca gag att agg agt 2*792

Leu Glu Ala Lys Gin Val Lys lie Arg I.ys Asn Ala Glu lie Arg Ser 425 430 435 aeg aca caa gcc aaa ate gta gca aag ggt gcc ctg tet att gag caa 2840

Thr Thr Gin Ala Lys He Val Ala Lys Gly Ala Leu Ser lie Glu Gin 440 445 450 aat geg aag etc gtc get aaa aag ata gat gtg gca aca gaa act eta 2888

Asn Ala Lys Leu Val Ala Lys Lys lie Asp Val Ala Thr Glu Thr Leu 455 460 465 470 act aat get ggg cgt att tat ggt cga gag gtt aag ett gac act aat 2936

Thr Asn Ala Gly Arg He Tyr Gly Arg Glu Val Lys Leu Asp Thr Asn 475 480 485 aat ttg att ait gat aaa gaa att tat get gaa egg aaa ttg agt att 2984

Asn Leu lie Asn Asp Lys Glu lie Tyx Ala Glu Arg Lys Leu Ser lie 490 495 500 ttg aeg aaa gga aaa gat ett gaa att att caa gat aga tat ttg tet 3032

Leu Thr Lys Gly Lys Asp Leu Glu lie lie Gin Asp Arg Tyr Leu Ser 505 510 515 cca ctg atg ege gta aaa agt agt gtc ege ttt tta ggc tet ccg ttt 3080

Pro Leu Met Arg Val Lys Ser Ser Val Arg Phe Leu Gly Ser Pro Phe 520 525 530 ttc tea ata tet ccg teg atg etc gca age ett agt gca cag ttt aag 3128

Phe Ser lie Ser Pro Ser Met Leu Ala Ser Leu Ser Ala Gin Phe Lys 535 540 545 550 cct ggt ttt gtg aat aag gga etc att gaa agt geg ggg agt gca gaa 3176

Pro Gly Phe Val Asn Lys Gly Leu lie Glu Ser Ala Gly Ser Ala Glu 555 560 565 tta act ttt aaa gaa aaa acc agt ttt tta aca gag ggc aat aat ttt 3224

Leu Thr Phe Lys Glu Lys Thr Ser Phe Leu Thr Glu Gly Asn Asn Phe 570 575 580 att aga get aaa gat geg tta ac 3247 lie Arg Ala Lys Asp Ala Leu 585 <210> 18 <211> 589 <212>蛋白質 <213>敗血性巴斯德样箱 <400> 18

Met Asn Lys Asn Arg Tyr Lys Leu lie Phe Ser Gin Val Lys Gly Cys 1 5 10 15 136852·序列表.doc .41 - IVi

Leu Val Pro Val Ala Glu Cys lie Asn Ser Ala lie Ser Asn Gly Ser 20 25 30 %

Ser Asp Ser Thr Ser Thr Ser Glu Gin Val Glu Glu Glu Pro Phe Leu 35 40 45

Leu Glu Gin Tyr Ser Leu Ser Ser Val Ser Leu Leu Val Lys Ser Thr 50 55 60

Phe Asn Pro Val Ser Tyr Ala Met Gin Leu Thr Trp Lys Gin Leu Ser 65 70 75 80 lie Leu Phe Leu Thr Val lie Ser Val Pro Val Leu Ala Glu Gly Lys 85 90 95

Gly Asp Glu Arg Asn Gin Leu Thr Val lie Asp Asn Ser Asp His lie 100 105 110

Lys Leu Asp Ala Ser Asn Leu Ala Gly Asn Asp Lys Thr Lys lie Tyr 115 120 125

Gin Ala Glu Asn Lys Val Leu Val lie Asp lie Ala Lys Pro Asn Gly 130 135 140

Lys Gly lie Ser Asp Asn Arg Phe Glu Lys Phe Asn lie Pro Asn Ser 145 150 155 160

Ala Val Phe Asn Asn Asn Gly Thr Glu Ala Gin Ala Arg Ser Thr Leu 165 170 175 lie Gly Tyr lie Pro Gin Asn Gin Asn Leu Arg Gly Gly Lys Glu Ala 180 185 190

Asp Val lie Leu Asn Gin Val Thr Gly Pro Gin Glu Ser Lys lie Val 195 200 205

Gly Ala Leu Glu Val Leu Gly Lys Lys Ala Asp lie Val He Ala Asn 210 215 220

Gin Asn Gly He Thr Leu Asn Gly Val Arg Thr lie Asn Ser Asp Arg 225 230 235 240

Phe Val Ala Thr Thr Ser Glu Leu lie Asp Pro Asn Gin Met Met Leu 245 250 255

Lys Val Thr Lys Gly Asn Val lie lie Asp lie Asp Gly Phe Ser Thr 260 265 270

Asp Gly Leu Lys Tyr Leu Asp lie lie Ala Lys Lys lie Glu Gin Lys 275 280 285

Gin Ser lie Thr Ser Gly Asp Asn Ser Glu Ala Lys Thr Asp Val Thr 290 295 300

Leu lie Ala Gly Ser Ser Glu Tyr Asp Leu Ser Lys His Glu Leu Lys 305 310 315 320

Lys Thr Ser Gly Glu Asn Val Ser Asn Asp Val lie Ala lie Thr Gly 325 330 335

Ser Ser Thr Gly Ala Met His Gly Lys Asn lie Lys Leu lie Val Thr 340 345 350 136852、序列表.doc -42-

l SI 1334884

Asp Lys Gly Ala Gly Val Lys His Asp Gly lie lie Leu Ser Glu Asn 355 360 365

Asp lie Gin lie Glu Met Asn Glu Gly Asp Leu Glu Leu Gly Asn Thr 370 375 380 lie Gin Gin Thr Val Val Lys Lys Asp Arg Asn lie Arg Ala Lys Lys 365 390 395 400

Lys lie Glu Val Lys Asn Ala Asn Arg Val Phe Val Gly Ser Gin Thr 405 410 415

Lys Ser Asp Glu lie Ser Leu Glu Ala Lys Gin Val Lys lie Arg Lys 420 425 430

Asn Ala Glu lie Arg Ser Thr Thr Gin Ala Lys lie Val Ala Lys Gly 435 440 445

Ala Leu Ser lie Glu Gin Asn Ala Lys Leu Val Ala Lys Lys lie Asp

450 455 460

Val Ala Thr Glu Thr Leu Thr A9n Ala Gly Arg lie Tyr Gly Arg Glu 465 470 475 480

Val Lys Leu Asp Thr Asn Asn Leu lie Asn Asp Lys Glu lie Tyr Ala 485 490 495

Glu Arg hys Leu Ser lie Leu Thr Lys Gly Lys Asp Leu Glu lie lie 500 505 510

Gin Asp Arg Tyr Leu Ser Pro Leu Met Arg Val Lys Ser Ser Val Arg 515 520 525

Phe Leu Gly Ser Pro Phe Phe Ser lie Ser Pro Ser Met Leu Ala Ser 530 535 540

Leu Ser Ala Gin Phe Lys Pro Gly Phe Val Asn Lys Gly Leu lie Glu 545 550 555 560

Ser Ala Gly Ser Ala Glu Leu Thr Phe Lys Glu Lys Thr Ser Phe Leu 565 570 575

Thr Glu Gly Asn Asn Phe lie Arg Ala Lys Asp Ala Leu 580 585

<210> 19 <211> 3247 <212> DNA <213>敗血性巴斯德桿菌 <220> <221> CDS <222> (1) .. (1446) <220> <223> fhaC <400> 19 gtc gac ctt gcg ggt gaa aag gta cct eta aat ttt ggc gat ate att 48

Val Asp Leu Ala Gly Glu Lys Val Ser Leu Asn Phe Gly Asp lie lie 1 5 10 15 -43- 136852-序列表.doc ISi 1334884 cat get tac caa aac cag ccc eta tea aca aaa gtt gtt ttt caa tta 96

His Ala Tyr Gin Asn Gin Pro Leu Ser Thr Lys Val Val Phe Gin Leu 20 25 30 gtg aaa gat ttg aeg gaa gtt tta tac cgt tet ggc tac gtg aca agt 144

Val Lys Asp Leu Thr Glu Val Leu Tyr Arg Ser Gly Tyr Val Thr Ser 35 40 45 gca att ggt eta aaa aat tea aaa ate age aat ggc gat ett gaa ttt 192

Ala He Gly Leu Lys Asn Ser Lys He Ser Asn Giy Asp Leu Glu Phe SO 55 60 att gta ctg tgg gga aga act ege gat ctg ttt gtg aat ggg gag aaa 240 lie Val Leu Trp Gly Arg Thr Arg Asp Leu Phe Val Asn Gly Glu Lys 65 70 75 80 cca acc cgt ttt aga gat aaa aca atg tta tea gtc eta ccc aat tta 288

Pro Thr Arg Phe Arg Asp Lys Thr Met Leu Ser Val Leu Pro Asn Leu 85 90 95

ate gga aat ege tta agt att cac gac att gac cag ttg ate gaa ate 33€

He Gly Asn Arg Leu Ser lie His Asp lie Asp Gin Leu lie Glu lie 100 105 110 tta aat act aeg aat aaa aaa gee aca gtg aat gtg gtt gca agt gaa 384

Leu Asn Thr Thr Asn Lys Lys Ala Thr Val Asn Val Val Ala Ser Glu 115 120 125 gaa aaa ggc age tea aat eta aat att gaa aga caa tat gat gtt ttt 432

Glu Lys Gly Ser Ser Asn Leu Asn lie Glu Arg Gin Tyr Asp Val Phe 130 135 140 ccg caa gtg agt gtc gga ttc aat aat tea ggt get ggc aat aat gee 480

Pro Gin Val Ser Val Gly Phe Asn Asn Ser Gly Ala Gly Asn Asn Ala 145 150 155 160 aat ggg cgt aat caa get aca ttg aat att get tgg agt gat eta tta 528

Asn Gly Arg Asn Gin Ala Thr Leu Asn lie Ala Trp Ser Asp Leu Leu 165 170 175 ggc aeg aat gat cgt tgg agt ttc teg agt agt tac cgt tta tat aaa 576

Gly Thr Asn Asp Arg Trp Ser Phe Ser Ser Ser Tyr Arg Leu Tyr Lys 180 185 190 aat cat cat get aac cag caa ege aat tat act ttg tet tac agt cag 624

Asn His His Ala Asn Gin Gin Arg Asn Tyr Thr Leu Ser Tyr Ser Gin 195 200 205 cct ata ggc ttt tet aca gta gaa att aaa gca teg gaa tet aeg tat 672

Pro lie Gly Phe Ser Thr Val Glu lie Lys Ala Ser Glu Ser Thr Tyr 210 215 220 gaa aaa gaa ett ege ggt ata aat act cat tet tet cat ggg aaa acc 720

Glu Lys Glu Leu Arg Gly lie Asn Thr His Ser Ser His Gly Lys Thr. 225 230 235 240 caa age tea get gtc aag ctg atg cat gtg tta ttg cgt aat aag gag 768

Gin Ser Leu Ala Val Lys Leu Met His Val Leu Leu Arg Asn Lys Glu 245 250 255 agt att tta tet aca tat acc gag ttc gag ttt aaa aaa egg att agt 816

Ser lie Leu Ser Thr Tyr Thr Glu Phe Glu Phe Lys Lys Arg lie Ser 260 265 270 44- 136852-序列表.doc tSl 1334884 tat ttt tct gat att ttg att ggg aaa tat cac aat aat aaa gtg age 864 Tyr Phe Ser Asp Tie Leu lie Gly Lys Tyr His Asn Asn T.ys Val Ser 275 280 285 gta ggg tta tct tac atg act aat ttt get tac ggg aag ett tac age 912 Val Oly Leu Ser Tvr Met Thr Asn Phe Ala Tvr Oly hys Leu Tyr Ser 290 295 — 300 · gac att get tac geg aat ggg ttg aga tgg ttt ggg geg aat tat tea 960 Asp lie Ala Tyr Ala Asn Gly Leu Arg Trp Phe Glv Ala Asn Tyr Ser 305 310 315 * 320 gca tat gat gca aat cgt gaa aaa acc tta aaa tta ttg tea gga agt 1008 Ala Tyr Asp Ala Asn Arg Glu Lys Thr Leu Lys Leu Leu Ser Gly Ser 325 330 335 att aat tgg cag cgt cca ata tcc ctg ttt gaa cgt geg atg aat tat 1056 He Asn Trp Gin Arg Pro lie Ser Leu Phe Glu Arg Ala Met Asn Tyr 340 345 350

caa tta cgt att ggt gcc caa tat ggt ttt gat agt ttg tat tct gaa 1104 Gin Leu Arg lie Gly Ala Gin Tyr Gly Phe Asp Ser Leu Tyr Ser Glu 355 360 365 aat caa ttt tea att ggt gat gaa tat aca gta aga gga ttt aaa ggt 1152 Asn Gin Phe Ser lie Gly Asp Glu Tyr Thr Val Arg Gly Phe Lys Gly 370 375 380 ggt geg gtt tct ggt gat agt ggt geg tat tta tea caa aca ctg aeg 1200 Gly Ala Val Ser Gly Asp Ser Gly Ala Tyr Leu Ser Gin Thr Leu Thr 385 390 395 400 gtt cct ttt tat cca caa aaa gca tat tta tct cag gta tcc cct ttt 1248 Val Pro Phe Tyr Pro Gin Lys Ala Tyr Leu Ser Gin Val Ser Pro Phe 405 410 415 att gga ttt gat atg ggt aaa gta cat att aaa tea aag cat aaa aca 1296 He Gly Phe Asp Met Gly Lys Val His lie Lys Ser Lys His Lys Thr 420 425 430

acc act tta gtc ggt ttt gcc eta ggc ttg aaa aeg caa ata aag tta 1344 Thr Thr Leu Val Gly Phe Ala Leu Gly Leu Lys Thr Gin lie Lys Leu 435 440 445 ttt tea tta tea tta acc tat gca caa cca atg aat ggt gtg agt ggt 1392 Phe Ser Leu Ser Leu Thr Tyr Ala Gin Pro Met Asn Gly Val Ser Gly 450 455 460 gtt aeg caa cat cgt caa aaa ccg att tat tat ttc tea gga tea ett 1440 Val Thr Gin His Arg Gin Lys Pro lie Tyr Tyr Phe Ser Gly Ser Leu 465 470 475 480 tct ttt taatctcttt taagtttaag gattaaetta atatgaacaa aaatcgttac 1496 Ser Phe aaactcattt ttagtcaagt caaaggttgt ctcgttcctg tggcagaatg tattaactca 1556 getattagea atggttcatc tgattcaaca tccacatcag aacaagttga agaggaacct 1616 ttccttctag aacaatattc actttcctcc gtgtctttat tagtaaaaag cacgttcaat 1676 cctgtttcgt atgcaatgca attgaettgg aaacagcttt ctattttatt tttaactgtg 1736 136852-序列表.doc isi 1334884

atttctgttc ctgttttggc tgagggaaaa ggggatgaaa gaaatcaatt aacagtgatt 1796 gataatagcg atcatattaa attagatgca tctaatcttg ctggtaatga taaaacaaaa 1856 atctatcaag cagaaaataa agttctggtt attgatattg ctaaaccaaa tgggaaaggg 1916 atttcagata accgttttga aaaatttaat attccaaata gcgcggtgtt taataataat 1976 gggactgaag cgcaggcaag atcaacatta attggttaca ttccgcaaaa tcaaaattta 2036 这aagaagctga tgttatatta aatcaagtga caggtcctca agaaagtaaa 2096 attgttggcg cgcttgaagt attaggtaaa aaagctgata tcgtcattgc aaaccaaaat 2156 ggtattacct taaatggtgt aagaacaata aattcagatc gttttgttgc cactacgagt 2216 gagcttatag atccgaatca gatgatgtta aaggttacaa aaggaaatgt gatcattgat 2276 attgatggtt tttcgacaga tggattaaag tatttagata ttattgctaa aaaaattgaa 2336 caaaagcaat caattacatc aggggataat tcagaagcaa aaacagatgt cactcttatt 2396 gcgggttcca gtgaatatga tttaagcaaa catgagctga aaaaaacgag cggtgaaaat 2456 gtatctaatg atgttattgc tatcacggga tctagtacag gcgcaatgca tggtaaaaat 2516 attaagttga ttgtgacaga taaaggtgca ggcgtaaaac atgatggaat tattttgtct 2576 gaaaatgata ttcagattga aatgaatgaa ggtgacttag aacttggcaa tacgattcag 2636 caaacagtgg taaaaaaaga ccgaaatatt cgagccaaga aaaaaattga agtgaaaaac 2696 gctaatcgtg tttttgttgg tagtcaaacg aaatcagatg aaatttcgtt agaggcgaaa 2756 caagttaaaa tcagaaaaaa cgcagagatt aggagtacga cacaagccaa aatcgtagca 2816 aagggtgccc tgtctattga gcaaaatgcg aagctcgtcg ctaaaaagat agatgtggca 2876 acagaaactc taactaatgc tgggcgtatt catggtcgag aggttaagct tgacactaat 2936 aatttgatta atgataaaga aatttatgct gaacggaaat tgagtatttt gacgaaagga 2996 aaagatcttg aaattattca agatagatat ttgtctccac tgatgcgcgt aaaaagtagt 3056 gtccgctttt taggctctcc gtttttctca atatctccgt cgatgctcgc aagccttagt 3116 gcacagttta agcctggttt tgtgaataag ggactcattg aaagtgcggg gagtgcagaa 3176 ttaactttta aagaaaaaac cagtttttta acagagggca ataattttat tagagctaaa 3236 gatgcgttaa c 3247 <210> 20 <211> 482 <212>蛋白質 c213>敗血性巴斯德样菌 <400> 20

Val Asp Leu Ala Gly Glu Lys Val Ser Leu Asn Phe Gly Asp lie lie 1 5 * 10 15 -46- 136852·序列表.doc

I SI 1334884

His A丄a Tyr Gin Asn Gin Pro Leu Ser Thr Lys Val Val Phe Gin Leu 20 25 30

Val Lys Asp Leu Thr Glu Val Leu Tyr Arg Ser Gly Tyr Val Thr Ser 35 40 45

Ala lie Gly Leu Lys Asn Ser Lys lie Ser Asn G丄y Asp Leu Glu Phe 50 55 60 lie Val Leu Trp Gly Arc Thr Arg Asp Leu Phe Val Asn Gly Glu Lys 65 70 75 80

Pro Thr Arg Phe Arg Asp Lys Thr Met Leu Ser Val Leu Pro Asn Leu 85 90 95 lie Gly Asn Arg Leu Ser lie His Asp He Asp Gin Leu lie Glu lie 100 105 110

Leu Asn Thr Thr Asn Lys Lys Ala Thr Val Asn Val Val Ala Ser Glu 115 120 125

Glu Lys Gly Ser Ser Asn Leu Asn lie Glu Arg Gin Tyr Asp Val Phe 130 135 140

Pro Gin Val Ser Val Gly Phe Asn Asn Ser Gly Ala Gly Asn Asn Ala 145 150 155 160

Asn Gly Arg Asn Gin Ala Thr Leu Asn lie Ala Trp Ser Asp Leu Leu 165 170 175

Gly Thr Asn Asp Arg Trp Ser Phe Ser Ser Ser Tyr Arg Leu Tyr Lys 180 185 190

Asn His His Ala Asn Gin Gin Arg Asn Tyr Thr Leu Ser Tyr Ser Gin 195 200 205

Pro He Gly Phe Ser Thr Val Glu lie Lys Ala Ser Glu Ser Thr Tyr 210 215 220

Glu Lys Glu Leu Arg Gly lie Asn Thr His Ser Ser His Gly Lys Thr 225 230 235 240

Gin Ser Leu Ala Val Lys Leu Met His Val Leu Leu Arg Asn Lys Glu 245 250 255

Ser lie Leu Ser Thr Tyr Thr Glu Phe Glu Phe Lys Lys Arg He Ser 260 265 270

Tyr Phe Ser Asp lie Leu lie Gly Lys Tyr His Asn Asn Lys Val Ser 275 280 285

Val Gly Leu Ser Tyr Met Thr Asn Phe Ala Tyr Gly Lys Leu Tyr Ser 290 295 300

Asp lie Ala Tyr Ala Asn Gly Leu Arg Trp Phe Gly Ala Asn Tyr Ser 305 310 315 320

Ala Tyr Asp Ala Asn Arg Glu Lys Thr Leu Lys Leu Leu Ser Gly Ser 325 330 335 lie Asn Trp Gin Arg Pro lie Ser Leu Phe Glu Arg Ala Met Asn Tyr 340 345 350 -47- 136852·序列表.doc i si 1334884

Gin Leu Arg lie Gly Ala Gin Tyr Gly Phe Asp Ser Leu Tyr Ser Glu 355 360 365

Asn Gin Phe Ser He Gly Asp Glu Tvr Thr Val Arg Glv Phe Lvs Gly 370 375 380 * ·

Gly Ala Val Ser Gly Asp Ser Gly Ala Tyr Leu Ser Gin Thr Leu Thr 385 390 395 400

Val Pro Phe Tyr Pro Gin Lys .via Tyr Leu Ser Gin Val Ser Pro Phe 405 410 415 lie Gly Phe Asp Met Gly Lys Val His lie Lys Ser Lys His Lys Thr 420 425 430

Thr Thr Leu Val Gly Phe Ala Leu Gly Leu Lys Thr Gin lie Lys Leu 435 440 445

Phe Ser Leu Ser Leu Thr Tyr Ala Gin Pro Met Asn Gly Val Ser Gly 450 455 460

Val Thr Gin His Arg Gin Lys Pro lie Tyr Tyr Phe Ser Gly Ser Leu 465 470 475 480

Ser Phe

<210> 21 <211> 1170 <212> DNA <213>敗血性巴斯德样菌 <220>

<221> CDS <222> (639) .. (1022) <220> <223> greA <400> 21

gtcaacaaac ggcaaccact tcggcaaaaa gcgattgcgc ttgtgttctg ctctaagctc 60 aagcgttggg atcgcgcgaa ttgcgttgac cactggtgag atcggggtca cctgtaaaac X20 gtacgataag atcgccatgc atttcattgt tttttatttt tccattggtt aatagactgg 180 tttcaaattg aaattggtca cttagtacga gtttggcggt taaggcggtg agcacttttt 240 gtgtactggc gggtaacata aaggtactgg cttggtgcgc tacaattttt tcattacgat 300 ttaagttttt agccacaaaa cctaggctgg tcccttcggg taaatgagcg ttgatttcag 360 caagatcaat ctcagcataa ctgaaatgac tgacgagtaa actacatata agtatcgttc 420 gtctgaaaag gcgtaaaagc gtggcagtaa aaaaagaaga tattttatac ataattggct 480 cgagcagttg ctattttttt attgtcgaac aataatagta tttgaaccct cgagagtaaa 540 tccttttctc gttaaacact tattttttta ttcaactacg gcattgtttt tacaatgttg 600 -48- 136852·序列表.doc

1 SI 1334884 tggttttgtt tttatctaaa aaggaagaaa aaacgatt atg aaa cag att cca atg 656

Met Lys Gin lie Pro Met 1 5 act ata cgt ggt gcg gaa caa tta aga caa gaa etc gat ett ttg aaa 704

Thr He Arg Gly Ala Glu Gin Leu Arg Gin Glu Leu Asp Phe Leu Lys 10 15 20 aac act cgt ege cca gaa att att aat get ace gca gaa get cgt gaa 752

Asn Thr Arg Arg Pro Glu lie lie Asn .Ma lie Ala Glu Ala Arg Glu 25 30 35 cat ggc gat eta aaa gaa aat gca gaa tac cat get gcg cgt gaa cag 800

His Gly Asp Leu Lys Glu Asn Ala Glu Tyr His Ala Ala Arg Glu Gin 40 45 50 caa gga ttt tgt gaa gga ega ate caa gaa att gaa ggg aaa tta gcg 848

Gin Gly Phe Cys Glu Gly Arg He Gin Glu lie Glu Gly Lys Leu Ala 55 60 65 70

aat agt caa att att gat gtc aca aag ate cca aat aat ggc aaa gtg 896

Asn Ser Gin lie lie Asp Val Thr Lys lie Pro Asn Asn Gly Lys Val 75 80 85 att ttt ggt gee aca att ttg tta ctg aat att gac aeg gaa gaa gaa 944 lie Phe Gly Ala Thr lie Leu Leu Leu Asn lie Asp Thr Qlu Glu Glu 90 95 100 gtc teg tac caa att gta ggc gat gat gaa gee aat att aaa gca ggg 992

Val Ser Tyr Gin lie Val Gly Asp Asp Glu Ala Asn lie Lys Ala Gly 105 110 115 eta att tea gtt aac gee aeg ega ttg aat tagagaaage taaatggatt 1042 Leu lie Ser Val Asn Ala Thr Arg Leu Asn 120 125 gcccaagatc ttggcgtcaa acaaacgtta attgacactt ccgtcattaa agegattaeg 1102 caaaatgcct taatggacga acaggcaaga attgagcaac atggcagtac accgaatact 1162 ttcgttga 1170

<210> 22 <211> 128 <212=>蛋白質 <213>敗血性巴斯德桿菌 <400> 22

Met Lys Gin He Pro Met Thr lie Arg Gly Ala Glu Gin Leu Arg Gin 1 5 10 15

Glu Leu Asp Phe Leu Lys Asn Thr Arg Arg Pro Glu He He Asn Ala 2〇 25 30

He Ala Glu Ala Arg Glu His Gly Asp Leu Lys Glu Asn Ala Glu Tyr 35 40 45

His Ala Ala Arg Glu Gin Gin Gly Phe Cys Glu Gly Arg lie Gin Glu 50 55 60

He Glu Gly Lys Leu Ala Asn Ser Gin lie He Asp Val Thr Lys He 65 70 75 80 -49- 136852·序列表.doc 1S1 1334884

Pro Asn Asn Gly Lys Val lie Phe 85 lie Asp Thr Glu Glu Glu Val Ser 100

Ala Asn lie Lys Ala Gly Leu lie 115 120

Gly Ala Thr lie Leu Leu Leu Asn 90 95

Tyr Gin lie Val Gly Asp Asp Glu 105 110

Ser Val Asn Ala Thr Arg Leu Asn 125

<210> 23 <211> 4666 <212> DNA <213>敗血性巴斯德样銪 <220>

<221> CDS <222> (980)·.《2440> <220> <223> guaB <400> 23 aacacatgaa cctcttcccc aaaatacgca tcatcaattc ctgcttgttc aaaatgttgt gcgatcaatt tattccgatc aatcgttggt tcaatttgct gtgtttgttt tgggaaagac tgttgattat tctgacgatt ggcgatttcg aatagcacat catttggcca ttttaatcca tctgcgattg ccatacccac tactaaactc gcccaataca aactcataat cacttgtcca cgtccacgtc ccgcagtttg atattctgct atattgtcaa gcaagaattg attggtcgag ggtgctaacg cttgcgtcaa ataagattca aaatgttgga cttgctgttc tatttgtatc aagatatctt gttctgaata acctaaaagt tagctagcga gtaatgcaag tacgttttgc tccatatctc gccaatcttg gcgaaatgct 60 aaacgtgcaa tcaactttct acctaattct 120 aatacttcaa tcagctctgc ccaaggttga 180 aaattaatgc caaagccaat cacgagatta 240 accaaaatcc ctgctaactt gcgcccatgt 300 atgttcaaag cacctgcttg ctttagcgtt 360 aagccttcta aattgaccct ttggtcacat 420 gcaaaaggag aaagccawtg acgaccacgt 480 aagcaaatag cgcctttccc caaatgtgca 540 ttaataatcg gcttaatata aagtgggtaa 600 tttaagcgac ttaattgagg tatgagacga 660 ccttgttgtt tcaatttrrc gatattgtgt 720 gcagtcaatt ctgctaaaga aaactgttga 7β0 ataaaaatcc ttatttarat aaccaaagag 840 aggcaactta ttatagacaa tgattttctc gaaaatcgat aaaaaaatcc attcccaaac 900 agcaacgaaa tctgtataat gcgaccgcaa tattttttac cctttta:二t ttcatatcaa 960 cctaagagag aatattgca atg tta cga gta ata aaa gaa gca tta acc ttc 1012

Met Leu Arg Val lie Lys Glu Ala Leu Thr Phe 15 10 gat gat gtt ttg ctt gtc cca gca cat tct act gtg etc zca aac acc 1060

Asp Asp Val Leu Leu Val Pro Ala His Ser Thr Val Leu Pro Asn Thr 15 20 25 •50· 136852·序列表.doc ί s] 1334884

gca gac ctt tcc act caa etc acc aaa act ate cgc etc aat ate cca 1108 Ala Asp Leu Ser Thr Gin Leu Thr Lys Thr lie Arg Leu Asn lie Pro 30 35 40 atg tta tcc gcc gcc atg gat acc gtg aca gaa act aaa ctg gca ate 1156 Met Leu Ser Ala Ala Met Asp Thr Val Thr Glu Thr Lys Leu Ala lie 45 50 55 tct ctt gca caa gaa ggt ggc ate ggg ttt att cat aaa aat atg tct 1204 Ser Leu Ala 31n Glu Gly Gly He Gly Phe lie His Lys Asn Met Ser 60 65 70 75 att gag cgt caa geg gaa cgt gtc cgc aaa gtg aaa aaa ttt gag age 1252 lie Glu Arg Gin Ala Glu Arg Val Arg Lys Val Lys Lys Phe Glu Ser 80 85 90 ggt att gta tcc gat cct gtc acc gtt tea cca acc tta tct tta gca 1300 Gly lie Val Ser Asp Pro Val Thr Val Ser Pro Thr Leu Ser Leu Ala 95 100 105 gaa tta agt gaa tta gtg aag aaa aat ggt ttt geg agt ttc cct gtt 1348 Glu Leu Ser Glu Leu Val Lys Lys Asn Gly Phe Ala Ser Phe Pro Val 110 115 120 gtt gat gat gaa aaa aat ctt gtc ggt ate att act ggt cgt gat aca 1396 Val Asp Asp Glu Lys Asn Leu Val Gly lie lie Thr Gly Arg Asp Thr 125 130 135 cgc ttt gtc aeg gat tta aat aaa aca gtg geg gac ttt atg acc cct 1444 Arg Phe Val Thr Asp Leu Asn Lys Thr Val Ala Asp Phe Met Thr Pro 140 145 150 155 aaa get cgt ctt gtc aeg gtg aaa cgc aat gca agt cgc gat gaa att 1492 Lys Ala Arg Leu Val Thr Val Lys Arg Asn Ala Ser Arg Asp Glu lie 160 165 170 ttt ggt eta atg cat aca cac cgt gta gaa aaa gtc ctt gtt gtc age 1540 Phe Gly Leu Met His Thr His Arg Val Glu Lys Val Leu Val Val Ser 175 180 185 gac gat ttc aaa tta aaa ggc atg ate acc tta aaa gac tac caa aaa 1588 Asp Asp Phe Lys Leu Lys Gly Met lie Thr Leu Lys Asp Tyr Gin Lys 190 195 200 tcc gag caa aaa cca caa gcc tgt aaa gat gaa ttt ggt cgt tta cgt 1636 Ser Glu Gin Lys Pro Gin Ala Cys Lys Asp Glu Phe Gly Arg Leu Arg 205 210 215 gtc ggt get gca gta gga gca gga cct ggt aat gaa gaa cgt att gat 1684 Val Gly Ala Ala Val Gly Ala Gly Pro Gly Asn Glu Glu Arg lie Asp 220 225 230 235 gca eta gtg aaa gca ggg gtc gat gtg tta ttg att gac cca tea cac 1732 Ala Leu Val Lys Ala Gly Val Asp Val Leu Leu He Asp Ser Ser His 240 245 250 ggt cat tea gaa ggt gtg tta caa cgt gtg c9t gaa act cgt geg aaa 1780 Gly His Ser Glu Gly Val Leu Gin Arg Val Arg Glu Thr Arg Ala Lys 255 260 265 cac cca gat ttg cca att gtt gca ggt aat gtg gca acc get gaa ggc 1Θ28 Tyr Pro Asp Leu Pro lie Val Ala Gly Asn Val Ala Thr Ala Glu Gly 270 275 280 136852·序列表.doc •51 - 1334884 gca att gcg ttg get gat gca ggg gca agt gca gtg aaa gtg ggg att 1876 Ala lie Ala Leu Ala Asp AJa G].y Ala Ser Ala Va]. Lyf? val Gly lie 285 290 295 ggt ect ggt tea att tgt aca aca cgt att gtc aca ggc gtg ggc gtt 1924 Gly Pro Glv Ser He Cys Thr Thr Ara lie Val Thr Gly Val Gly Val 300 * 305 " 310 315 cca caa att aca gcg att gcc gat gcg gca gaa gca eta aaa gat egg 1972 Pro Gin lie Thr Ala lie Ala Asp Ala Ala Glu Ala Leu Lys Asp Arg 320 325 330 ggt att cct gtg att gca gat ggc ggt ate cgt ttc tet ggt gat att 2020 Gly lie Pro Val lie Ala Asp Gly Gly lie Ara Phe Ser Gly Asp lie 335 340 345 teg aaa gcc att gcg gcg ggc gcc tet tgt gtt atg gtg ggt tee atg 2068 Ser Lys Ala lie Ala Ala Gly Ala Ser Cys Val Met Val Gly Ser Met 350 355 360

ttt gca ggt aca gaa gaa gca cca ggt gaa ate gaa ett tat caa ggt 21X6 Phe Ala Gly Thr Glu Glu Ala Pro Gly Glu lie Glu Leu Tyr Gin Gly 365 370 375 cgt gcc ttt aaa tet tat ega ggt atg gga teg tta ggt gcg atg age 2164 Arg Ala Phe Lys Ser Tyr Arg Gly Met Gly Ser Leu Gly Ala Met Ser 380 385 390 395 aaa ggc tea age gac ege tat ttc cag tee gat aat gca get gac aaa 2212 Lys Gly Ser Ser Asp Arg Tyr Phe Gin Ser Asp Asn Ala Ala Asp Lys 400 405 410 tta gta cca gaa ggt att gaa gga cgt att cca tat aaa gga ttc tta 2260 Leu Val Pro Glu Gly lie Glu Gly Arg lie Pro Tyr Lys Gly Phe Leu 415 420 425 aaa gaa att ate cat caa caa atg ggt gga ttg cgt tet tgt atg ggc 2308 Lys Glu lie lie His Gin Gin Met Gly Gly Leu Arg Ser Cys Met Gly 430 435 440 tta aeg ggt tgt gca acc att gat gaa etc cgt acc aaa gcg cag ttt 2356 Leu Thr Gly Cys Ala Thr lie Asp Glu Leu Arg Thr Lys Ala Gin Phe 445 450 455

gtg ege att agt ggt gca ggg ate caa gaa age cat gtg cat gat gtg 2404 Val Arg lie Ser Gly Ala Gly lie Gin Glu Ser His Val His Asp Val 460 465 470 475 act ate aca aaa gaa gcc cct aat tat cgt atg ggt taaacattgc 2450

Thr lie Thr Lys Glu Ala Pro Asn Tyr Arg Met Gly 480 485 ttaggtgggg attatcccca cctaagttta ttttaaataa caacgttaat agagaagett 2510 atttttatgt atggcattaa aattaaaaat gttattaaac tttttctatt aaagttttta 2570 agaaataaat ategatataa aatcaatatt caacatcatc tcattagtat tgaaggaaaa 2630 tgcggtgagt ttgattcaag ccagctaaac tatgtttatt tggtgaaaga tcctgagata 2690 agaaataatc gactgacact gtacttgaat gattttttca aaattggggt aaattatcat 2750 qgatttactc aaatqtatca aacactatca tccaaqtatg gttttgatga cgcaacqttt 2810 •52- 136852·序列表.doc ί Si 1334884

tttgaatatc tttgtaagaa agggcctttt tctattcaaa tttggcgtaa aaaacaaact 2870 caaaattatg tgattcttga tgaagcctat actgactata cacaaggttt tgaaattcaa 2930 tctcctgaaa aaatatttat tccttggggg actacttatg aagcctcatt tcagcagaca 2990 caatttaaag aaaaaggaat ctcttatgac tttatcttcc ctattcggat agggcgttta 3050 ttactcaagg atgtgtggat cacaccaagt gtccgaaaag atgtaccgat tttagcgtta 3110 tacactgagt gctatcatga atccgcgaca gaaaaaagtt atcaggaatt aaccgccgca 3170 ttacgtgaga accaacagtt aatcagatca tgtgttgaag aacgagccga tccgaaatta 3230 tataagtctg ttttacgcct taacgcgaca gaatttgaat tgcgttatta tcgacatata 3290 cgagacgatt ttgatagggg atacactaaa ttcagcatta gagatacgac agactattta 3350 gattatgtga tcaacgagcc ttatgaaaat caattagtga taacggatta tttagtgatt 3410 gaggcgcaaa atttaataaa aatggattat accgataatt ccattattaa acgccgacca 3470 ccaaaaataa aagaaaagtt tcgtgatgca caaagcctga tttggacaga tgatctcaat 3530 cataaaatcg gttttaccag tgatgaccgc gctatcgtct ttgataaagc ggacattgaa 3590 tcctttactc tggcaaatat agagacaacc cgaagacata atqgcagttc actcagcatc 3650 tgttttgtgg ataaaaataa ggaagccatc actgtatttc tagctgaaca tcattttctg 3710 ataccctatg tggataaaat aaaaacactg acacaaaaag aggtgttgtt tcttgaagaa 3770 tatatagagg atgtttaaaa aaccgataac atcttgatgt catcgcaaat tcaaactcct 3830 tttacacaat atatttaaac tttaacccga tttaatactt acgtaaaaac aactaagaga 3890 acttaaatga acaacattca caaccataaa attttaattt tggacttcgg ttcacaatat 3950 acccagttga ttgcccgccg tgtacgtgaa attggcgtgt actgcgaact ttgggcatgg 4010

ttctggtggt 4070 caacgccggt 4130 aggtggttta 4190 agccgcggac 4250 agacgtttgg 4310 cgcaatgacc 4370 tgtccaattc 4430 tgtggtgaag 4490 cgttgctcgt 4550 cgttaactgc 4610 cgttga 4666 gatgtatccg cctgaaagta gtacccgtat actgaaactt gcgttatttg atgagccatg tcaacctgtc cacccagaag atctgtggtt cttaaagcac cgtatzaggca aagccgatat ccactgaaga tagggatctg ctactcaccg ctcaattaaa gcgataaagt caatcgcagc tgactcatac gtgaacgtaa aagtgggcga gcttagaaaa tcgtgagttt aaacagccca ttatggtatg agaatttggt tgatgatgtc gacgcgtttg tatgtcggat aaaaagcggg ctggacacca tgatgaagtg agtgtccgcg aatccaactg cgagctcccg caaaccatgg tatgcatcag gcaagttcac ccagatcatt gaacgtcgtc cttgaattat gaaaatatca attttaggct ctcacgttaa ggattattct aatacgtatt cgatgcaact tgaatctaaa agcccaaatt tccaagtcac gtttttatgg taacgaattt ttgaagatgc tatctggtgg tccccgtaag -53- Π6852·序列表.doc

l SI 1334884 <21〇> 24 <211> 487 <2W>蛋白質 <213>敗血性巴斯德桿菌 <400> 24

Met Leu Arg Val He Lys Glu Ala Leu Thr Phe Asp Asp Val Leu Leu 1 5 10 15

Val Pro Ala His Ser Thr Val Leu Pro Asn Thr Ala Asp Leu Ser Thr 20 25 30

Gin L.eu Thr Lys Thr lie Arg Leu Asn lie Pro Met Leu Ser Ala Ala 35 40 45

Met Asp Thr Val Thr Glu Thr Lys Leu Ala lie Ser Leu Ala Gin Glu 50 55 60

Gly Gly lie Gly Phe lie His Lys Asn Met Ser lie Glu Arg Gin Ala 65 70 75 80

Glu Arg Val Arg Lys Val Lys Lys Phe Glu Ser Gly lie Val Ser Asp 85 90 95

Pro Val Thr Val Ser Pro Thr Leu Ser Leu Ala Glu Leu Ser Olu Leu 100 105 110

Val Lys Lys Asn Gly Phe Ala Ser Phe Pro Val Val Asp Asp Glu Lys 115 120 125

Asn Leu Val Gly lie lie Thr Gly Arg Asp Thr Arg Phe Val Thr Asp 130 135 140

Leu Asn Lys Thr Val Ala Asp Phe Met Thr Pro Lys Ala Arg Leu Val 145 ISO 155 160

Thr Val Lys Arg Asn Ala Ser Arg Asp Glu lie Phe Gly Leu Met His 165 170 175

Thr His Arg Val Glu Lys Val Leu Val Val Ser Asp Asp Phe Lys Leu 180 185 190

Lys Gly Met lie Thr Leu Lys Asp Tyr Gin Lys Ser Glu Gin Lys Pro X95 200 205

Gin Ala Cys Lys Asp Glu Phe Gly Arg Leu Arg Val Gly Ala Ala Val 210 215 220

Gly Ala Gly Pro Gly Asn Glu Glu Arg lie Asp Ala Leu Val Lys Ala 225 230 235 240

Gly Val Asp Val Leu Leu lie Asp Ser Ser His Gly His Ser Glu Gly 245 250 255

Val Leu Gin Arg Val Arg Glu Thr Arg Ala Lys Tyr Pro Asp Leu Pro 260 265 270

He Val Ala Gly Asn Val Ala Thr Ala Glu Gly Ala lie Ala Leu Ala 275 280 285

Asp Ala Gly Ala Ser Ala Val Lys Val Gly He Gly Pro Gly Ser lie 290 295 300 -54· 136852·序歹11表.doc 151 1334884

Cys Thr Thr Arg lie Val Thr Gly Val Gly Val Pro Gin lie Thr Ala 305 310 315 320 lie Ala Asp Ala Ala Glu Ala Leu Lys Asp Arg Gly lie Pro Val lie 325 330 335

Ala Asp Gly Gly lie Arg Phe Ser Gly Asp He Ser Lys Ala lie Ala 340 345 350

Ala Gly Ala Ser Cys Val Met Val Gly Ser Met Phe Ala Gly Thr Glu 355 - 360 365

Glu Ala Pro Gly Glu He Glu Leu Tyr Gin Gly Arg Ala Phe Lys Ser 370 375 360

Tyr Arg Gly Met Gly Ser Leu Gly Ala Met Ser Lys Gly Ser Ser Asp 385 390 395 400

Arg Tyr Phe Gin Ser Asp Asn Ala Ala Asp Lys Leu Val Pro Glu Gly 405 410 415 ·

lie Glu Gly Arg lie Pro Tyr Lys Gly Phe Leu Lys Glu lie lie His 420 425 430

Gin Gin Met Gly Gly Leu Arg. Ser Cys Met Gly Leu Thr Gly Cys Ala 435 440 445

Thr lie Asp Glu Leu Arg Thr Lys Ala Gin Phe Val Arg lie Ser Gly 450 455 460

Ala Gly lie Gin Glu Ser His Val His Asp Val Thr He Thr Lys Glu 465 470 475 480

Ala Pro Asn Tyr Arg Met Gly 485

<210> 25 <211> 2364 <212> DNA <213>故血性巴斯德桿菌 <220>

<221> CDS <222> (191) . . (1828) <220> <223> ΗΪ1501 <400> 25 gtcaacactc atcgcacagc tgaggcattt cccgaaagct gatcatgatg atggacctga 60 tgcgctagag atgctgtgga aaaatgcggt aagcagctct gccccgattg agttcatgac 120 aattgacggc gatttagggc gtgatgaatt tgatgacggc gatttataca gtatttggcg 180 gagataaaaa atg gcg aag aaa aag aaa aaa tta caa caa gcg aaa aaa 229 Met Ala Lys Lys Lys Lys Lys Leu Gin Gin Ala Lys Lys 15 10 gta caa gtt ggc tta gat aca caa aca aat gag gcg cgt gtc acg gag 277

Val Gin Val Gly Leu Asp Thr Gin Thr Asn Glu Ala Arg Val Thr Glu 15 20 25 •55- 136852-序列表.doc 1334884

aca gga aga att att tet gat cac cca age aat aaa att acc ccc gca 325 Thr Gly Arg lie lie Ser Asp His Pro Ser Asn Lys lie Thr Pro Ala 30 35 40 45 aag tta aaa ggg att tta gaa gat get gaa ggt ggt gat att acc geg 373 Lys Leu Lys Gly lie Leu Glu Asp Ala Glu Gly Gly Asp lie Thr Ala 50 55 €0 caa cat gag ctt ttc atg gat att gaa gaa ege gac agt tgc ate ggg 421 Gin His Glu Leu Phe Met Asp ：：e Glu Glu Arg Asp Ser Cvs He Gly 65 70 75 gca aat att caa acc cgt aag cgt geg att tta acc ctt gac tgg ege 469 Ala Asn lie Gin Thr Arg Lys Arg Ala He Leu Thr Leu Asp Trp Arg 80 85 90 att gca gag cca cgt aat gcc aca ccg caa gaa gaa aaa ctg caa gtc 517 lie Ala Glu Pro Arg Asn Ala Thr Pro Gin Glu Glu Lys Leu Gin Val 95 100 105 gaa att gac gag ctt ttc tat caa ttc cca atg eta gaa gat tta atg 565 Glu lie Asp Glu Leu Phe Tyr Gin Phe Pro Met Leu Glu Asp Leu Met 110 115 120 125 gtg gat atg atg gat geg gta gga cat ggt ttt teg geg tta gaa a*-t 613 Val Asp Met Met Asp Ala Val Gly His Gly Phe Ser Ala Leu Glu lie 130 135 140 gaa tgg aag caa get gaa agt aaa tgg att cca gtt aat ttt ate gca 661 Glu Trp Lys Gin Ala Glu Ser Lys Trp lie Pro Val Asn Phe lie Ala 145 150 155 cgt ccg cag teg tgg ttt aaa eta gac aag gat gat aat tta ctg ctt 709 Arg Pro Gin Ser Trp Phe Lys Leu Asp Lys Asp Asp Asn Leu Leu Leu 160 165 170 aaa acg cca gat aat caa gac ggt gag ccg ttg aga caa tat ggc tgg 757 Lys Thr Pro Asp Asn Gin Asp Gly Glu Pro Leu Arg Gin Tyr Gly Trp 175 180 185 gta gtg cat acc cac aaa tea aga aca gta cag ctt get cgt atg ggt 805 Val Val His Thr His Lys Ser Arg Thr Val Gin Leu Ala Arg Met Gly 190 195 200 205 tta ttt aga acg etc gca tgg ctt tat atg ttt aaa cac tac teg gtg 8S3 Leu Phe Arg Thr Leu Ala Trp Leu Tyr Met Phe Lys His Tyr Ser Val 210 215 220 cat gat ttt gcc gaa ttt eta gag ctt tat ggt atg ccg att cgt att 901 His Asp Phe Ala Glu Phe Leu Glu Leu Tyr Gly Met Pro lie Arg lie 225 230 235 ggt aaa tac cca ttt ggg gca acg aat gac gaa aag ege aca tta ttg 949 Gly Lys Tyr Pro Phe Gly Ala Thr Asn Asp Glu Lys Arg Thr Leu Leu 240 245 250 cgt gca ctt get caa ate gga cat aac gca gca ggg att atg cca gaa 997 Arg Ala Leu Ala Gin He Gly His Asn Ala Ala Gly lie Met Pro Glu 255 260 26S gga atg aat gtt gag ttg cat aat gtg aca aac act act ggc teg get 1045 Gly Met Asn Val Glu Leu His Asn Val Thr Asn Thr Thr Gly Ser Ala 270 275 280 285 136852-序列表.doc -56- gga age aac ccg ctt ttg caa atg gtg gac tgg tgt gaa aag tcc gcc 1093 Gly Ser Asn Pro Phe 290 Leu Gin Net Val Asp 295 Trp Cys Glu Lys Ser 300 Ala gca cgt ttg att eta ggg caa aca tta aca age ggt gca gat ggt aaa 1141 Ala Arg Leu lie 005 Leu Gly Gin Thr Leu 310 Thr Ser Gly Ala Asp Gly Lys 315 act tea act aat gcc ctt gga caa gtg cat aat gaa gtc aga cgt gac 11Θ9 Thr Ser Thr 320 Asn Ala Leu Gly Gin 325 Val His Asn Glu Val Arg Arg Asp 330 ttg ctt gtg tct gat get aaa cag att gca caa act att aca caa cag 1237 Leu Leu 335 Val Ser Asp Ala Lys 340 Gin lie Ala Gin Thr 345 He Thr Gin Gin att att ctg cca tat ctt caa att aac att gat ccg aat att ttg cct 1285 lie 350 lie Leu Pro Tyr Leu 355 Gin lie Asn lie Asp 360 Pro Asn lie Leu Pro 365 tct cgt gtg ccg tat ttc gag ttt gac aeg aaa gaa tat get gat tta 1333 Ser Arg Val Pro Tyr 370 Phe Glu Phe Asp Thr 375 Lys Glu Tyr Ala Asp 380 Leu agt gtc eta geg gat get att cct aag ctt gtg age gta gga gtg ege 1381 Ser Val Leu Ala 385 Asp Ala lie Pro Lys 390 Leu Val Ser Val Gly Val 395 Arg att cct gaa aat tgg gtg cgt gat aaa geg ggc att cca gaa ccg cag 1429 lie Pro Glu 400 Asn Trp Val Arg Asp 405 Lys Ala Gly lie Pro 410 Glu Pro Gin gaa aat gaa aeg att tta agt geg gtt caa cat gat ttt aaa aca gat 1477 Glu Asn 415 Glu Thr He Leu Ser 420 Ala Val Gin His Asp 425 Phe Lys Thr Asp tta aac gat gtt gaa aat ccg aaa aaa cag acc gca ctt tct gta caa 1525 Leu 430 Asn Asp Val Glu Asn 435 Pro Lys Lys Gin Thr 440 Ala Leu Ser Val Gin 445 aat cac gtg aca ggt tgt cag tgt gat ggc tgt cgt ggt gtt gca tta 1573 Asn His Val Thr Gly 450 Cys Gin Cys Asp Gly Cys 455 Arg Gly Val Ala 460 Leu tct geg aat aat aac agt tct act geg cag ggc gtg eta gat ggt gga 1621 Ser Ala Asn Asn 465 Asn Ser Ser Thr Ala 470 Gin Gly Val Leu Asp 475 Gly Gly ctt geg caa gca ttt aat gag cct gat ttt aat aaa caa tta aat cca 1669 Leu Ala Gin 480 Ala Phe Asn Glu Pro Asp 485 Phe Asn Lys Gin 490 Leu Asn Pro atg gta aag aaa get gtt geg gta etc atg gca tgt gac tct tac gat 1717 Met Val 495 Lys Lys Ala Val Ala 500 Val Leu Met Ala Cys 505 Asp Ser Tyr Asp gag geg gca gaa aaa etc get gaa gca tac cca gaa att tea agt cac 1765 Glu 510 Ala Ala Glu Lys Leu 515 Ala Glu Ala Tyr Pro 520 Glu lie Ser Ser His 525 gaa cac gaa cag tat etc tea aat geg ctg ttt tta get gat tta ctt 1813 Glu His Glu Gin Tyr S30 Leu Ser Asn Ala Leu 535 Phe Leu Ala Asp Leu 540 Leu 136852-序列表.doc -57- 1334884 gga gga act aat gtc taaaccgctt agttttctat tcggacttga accaacgcaa 1868 Gly Gly Thr Asn Val 54ϋ gccactgagt tcttacataa caaaaaatta cttgcaacga aagtgttcaa aaaatcactg 1928 catgatagtg ccatcgcaag agctacaaca atcgcgagat tatctagtct tgagatgacg 1988 aargatattt ataaatcaat ggaagttgcc aaaaaagagg gtaagagctt tacacaatgg 2048 aaaaaagact tggtaagtga gtttgagaaa aaaggctggg tattcgggca tgataaatct 2108 atcagtcgcg gtatcgacgg aaaactgttg gctgatccga aaacaggcga atattttggt 2168 acaccgcgtc ggctgaatac aatttatcgt acaaacgtgc aagccgcata ttctgcggcg 2228 cgctatcagc gcatgatgga taatattgat catcgcccct attggcaata ttccgctgtc 2288 agcgatgagc gtacacgacc ctctcatctt gcactaaacg gtcgaattta tcgctatgat 234Θ

gacccgtttt ggtcga 2364 <210> 26 <211> 546 <212>蛋白質 <213>敗血性巴斯德桿菌 <400> 26

Met Ala Lys Lys Lys Lys Lys Leu Gin Gin Ala Lys Lys Val Gin Val 15 10 15

Gly Leu Asp Thr Gin Thr Asn Glu Ala Arg Val Thr Glu Thr Gly Arg " 20 25 30 lie lie Ser Asp His Pro Ser Asn Lys lie Thr Pro Ala Lys Leu Lys 35 40 45

Gly lie Leu Glu Asp Ala Glu Gly Gly Asp lie Thr Ala Gin His Glu 50 55 60

Leu Phe Met Asp lie Glu Glu Arg Asp Ser Cys lie Gly Ala Asn lie 65 70 75 80

Gin Thr Arg Lys Arg Ala lie Leu Thr Leu Asp Trp Arg lie Ala Glu 85 90 95

Pro Arg Asn Ala Thr Pro Gin Glu Glu Lys Leu Gin Val Glu lie Asp 100 105 110

Glu Leu Phe Tyr Gin Phe Pro Met Leu Glu Asp Leu Met Val Asp Met 115 120 125

Met Asp Ala Val Gly His Gly Phe Ser Ala Leu Glu lie Glu Trp Lys 130 135 140

Gin Ala Glu Ser Lys Trp lie Pro Val Asn Phe lie Ala Arg Pro Gin 145 150 155 160

Ser Trp Phe Lys Leu Asp Lys Asp Asp Asn Leu Leu Leu Lys Thr Pro 165 170 175 • 58 - 136852·序列表.doc I S3 1334884

Asp Asn Gin Asp Gly Glu Pro Leu Arg Gin Ty*r Gly Trp Val Val Hie 180 185 190

Thr His Lys Ser Arg Thr Val Gin Leu Ala Arg Met Gly Leu Phe Arg 195 200 205

Thr Leu Ala Trp Leu Tyr Met Phe Lys His Tyr Ser Val His Asp Phe 210 215 220

Ala Glu Phe Leu Glu Leu Tyr Gly Met Pro lie Arg lie Gly Lys Tyr 225 230 235 240

Pro Phe Gly Ala Thr Asn Asp Glu Lys Arg Thr Leu Leu Arg Ala Leu 245 250 255

Ala Gin lie Gly His Asn Ala Ala Gly lie Met Pro Glu Gly Met Asn 260 265 270

Val Glu Leu His Asn Val Thr Asn Thr Thr Gly Ser Ala Gly Ser Asn 275 280 285

Pro Phe Leu Gin Met Val Asp Trp Cys Glu Lys Ser Ala Ala Arg Leu 290 295 300 lie Leu Gly Gin Thr Leu Thr Ser Gly Ala Asp Gly Lys Thr Ser Thr 305 310 315 320

Asn Ala Leu Gly Gin Val His Asn Glu Val Arg Arg Asp Leu Leu Val 325 330 335

Ser Asp Ala Lys Gin lie Ala Gin Thr lie Thr Gin Gin lie lie Leu 340 345 350

Pro Tyr Leu Gin lie Asn lie Asp Pro Asn lie Leu Pro Ser Arg Val 355 360 365

Pro Tyr Phe Glu Phe Asp Thr Lys Glu Tyr Ala Asp Leu Ser Val Leu 370 375 380

Ala Asp Ala lie Pro Lys Leu Val Ser Val Gly Val Arg lie Pro Glu 385 390 395 400

Asn Trp Val Arg Asp Lys Ala Gly lie Pro Glu Pro Gin Glu Asn Glu 405 410 415

Thr lie Leu Ser Ala Val Gin His Asp Phe Lys Thr Asp Leu Asn Asp 420 425 430

Val Glu Asn Pro Lys Lys Gin Thr Ala Leu Ser Val Gin Asn His Val 435 440 445

Thr Gly Cys Gin Cys Asp Gly Cys Arg Gly Val Ala Leu Ser Ala Asn 450 455 460

Asn Asn Ser Ser Thr Ala Gin Gly Val Leu Asp Gly Gly Leu Ala Gin 465 470 475 480

Ala Phe Asn Glu Pro Asp Phe Asn Lys Gin Leu Asn Pro Met Val Lys 485 490 495

Lys Ala Val Ala Val Leu Met Ala Cys Asp Ser Tyr Asp Glu Ala Ala 500 505 510 •59· 130852-序列表.doc 131 1334884

Glu Lys Leu Ala Glu Ala Tyr Pro Glu lie Ser Ser His Glu His Glu 515 520 S25

Gin Tyr Leu Ser Asn Ala Leu Phe Leu Ala Asp Leu Leu Glv Glv Thr 530 535 540 ' "

Asn Val 545 <210> 27 <211> 1353 <212> DNA <213>敗血性巴斯德桿菌 <220>

<223> hmbR <220> <221> CDS <222> (2)..(1351) <220> <221> misc_#et <222> 125 — <223> Xaa=任何或未知的胺基酸 <220> <221> inisc—特徵 <222> 133 ~ <223> kaa=任何或未知的胺基酸 <220> <221> misc 特徵 <222> 141 ~ <223> Xaa=任何或未知的胺基酸 <220> <221> m丨sc特徵 <222> 15]- <223> Xaa=任何或未知的胺基酸 <400> 27 g tea aeg aaa gtc ggt tac gat att aat aac act cat cgt ttt aca ctg Ser Thr Lys Val Gly Tyr Asp lie Asn Asn Thr His Arg Phe Thr Leu 1 5 10 15 ttt tta gaa gat ege cgt gaa aag aag ett acc gaa gaa aaa aca tta Phe Leu Glu Asp Arg Arg Glu Lys Lys Leu Thr Glu Glu Lys Thr Leu . 20 25 30 ggg ett agt gat gca gtg cgt ttt get aat gat caa acc cct tat etc Gly Leu Ser Asp Ala*Val Arg Phe Ala Asn Asp Gin Thr Pro Tyr Leu 35 40 45 cgt tat ggt att gaa tat ega tat aac ggc ttg tet tgg ttg gaa aeg Arg Tyr Gly lie Glu Tyr Arg Tyr Asn Gly Leu Ser Trp Leu Glu Thr SO 55 60 gta aag ett ttt ttg gca aag cag aaa ate gaa caa cgt tet get etc Val Lys Leu Phe Leu Ala Lys Gin Lys He Glu Gin Arg Ser Ala Leu 65 70 75 80 49 97 145 193 •60· 136852-序列表.doc 1 241 1334884 caa gag ttt gat att aat aat agg aat aaa ttg gat teg act atg teg 289

Gin Glu Phe Asp He Asn Asn Arg Asn Lys Leu Asp Ser Thr Met Ser 85 90 95 ttt gta tat tta caa aga cag aat ata get egg gga gaa ttt tea aeg 337

Phe Val Tyr Leu Gin Arg Gin Asn lie Ala Arg Gly Glu Phe Ser Thr 100 105 110 agt cct tta tat tgg ggg ccg agt ege cat cgt tta tnt geg aaa ttc 385

Ser Pro Leu Tyr Trp Gly Pro Ser Arg His Arg Leu Xaa Ala Lys Phe 115 120 125 gaa ttt cgt gat ang ttt tta gaa aat atg aat aag ent ttt aeg ttt 433

Glu Phe Arg Asp Xaa Phe Leu Glu Asn Net Asn Lys Xaa Phe Thr Phe 130 135 140 egg ccg tgg caa ate aat ana ttc aga caa caa ggt ega aat aac tat 481

Arg Pro Trp Gin lie Asn Xaa Phe Arg Gin Gin Gly Arg Asn Asn Tyr 145 150 155 160

aca gaa gtg ttt ccc gtt aaa tee ega gag ttt tet ttt tet ett atg 529

Thr Glu Val Phe Pro Val Lys Ser Arg Glu Phe Ser Phe Ser Leu Met 165 170 175 gac gac att aag att ggc gaa ttg eta cat etc gga ttg ggc ggt egg 577

Asp Asp lie Lys He Gly Glu hen Leu His Leu Gly Leu Gly Gly Arg 180 185 190 tgg gat cac tat aac tat aag cca tta tta aat tet cag cat aat ate 625

Trp Asp His Tyr Asn Tyr Lys Pro Leu Leu Asn Ser Gin His Asn lie 195 200 205 aac agg aca cag aga tta cct tat cca aaa aca tea tee aaa ttt teg €73

Asn Arg Thr Gin Arg Leu Pro Tyr Pro Lys Thr Ser Ser Lys Phe Ser 210 215 220 tat caa ttg agt tta gag tat caa tta cat cca tea cat caa att gca 721

Tyr Gin Leu Ser Leu Glu Tyr Gin Leu His Pro Ser His Gin He Ala 225 230 235 240 tac cgt tta agt acc ggt ttt agg gtt ccc cgt gtt gaa gat ett tat 769

Tyr Arg Leu Ser Thr Gly Phe Arg Val Pro Arg Val Glu Asp Leu Tyr 245 250 255

ttt gaa gac ega gga aaa agt tet tea caa ttt ett cct aac ccc gat 817

Phe Glu Asp Arg Gly Lys Ser Ser Ser Gin Phe Leu Pro Asn Pro Asp 260 265 270 eta caa ccg gaa act gca ctg aat cat gaa ata agt tac cgt ttc caa. 865

Leu Gin Pro Glu Thr Ala Leu Asn His Glu lie Ser Tyr Arg Phe Gin 275 260 285 aat caa tat gee cat ttc age gtc ggg ett ttc cgt aca cgt tat cat 913

Asn Gin Tyr Ala His Phe Ser Val Gly Leu Phe Arg Thr Arg iyr His 290 295 300 aac ttt att caa gaa cgt gag atg acc tgt gat aaa att cca tat gag 961

Asn Phe He Gin Glu Arg Glu Met Thr Cys Asp Lys lie Pro Tyr Glu 305 310 315 320 cat aat agg act tat gga tat tgc aeg cat aat act tat gta atg ttt 1009

Tyr Asn Arg Thr Tyr Gly Tyr Cys Thr His Asn Thr Tyr Val Met Phe 325 330 335 -61 - 136852-序列表.doe isi 10571334884

gtt aat gaa cct gaa gcc gtg att aaa ggg gtt gaa gta age ggt get

Val Asn Glu Pro Glu Ala Val lie Lys Gly VaX Glu Val Ser Gly Ala 340 345 350 tta aat ggg teg gca ttc gga ett tee gac ggt tta act ttc cgt etc

Leu Asn Gly Ser Ala Phe Gly Leu Ser Asp Gly Leu Thr Phe Arg Leu 355 360 365 aaa ggg age Cac age aaa ggt caa aat cat gac ggc gat ccg tta aaa

Lys Gly Ser Tyr Ser Lys Gly Gin Asn His Asp Gly Asp Pro Leu Lys 370 375 380 tet att caa cca tgg aca gtg gta acc ggt att gat tac gaa act gaa

Ser He Gin Pro Trp Thr Val Val Thr Gly lie Asp Tyr Glu Thr Glu 3B5 390 395 400 ggg tgg age gtg agt ttg age ggg cgt tat agt geg get aaa aaa gee

Gly Trp Ser Val Ser Leu Ser Gly Arg Tyr Ser Ala Ala Lys Lys Ala 405 410 415 aaa gat geg ata gaa aeg gaa tac aca cat gat aaa aag gtt gtc aaa

Lys Asp Ala lie Glu Thr Glu Tyr Thr His Asp Lys Lys Val Val Lys 420 425 430 caa tgg ccg cat tta agt cca tee tac ttt gtt gtt gat ttt aeg ggg

Gin Trp Pro His Leu Ser Pro Ser Tyr Phe Val Val Asp Phe Thr Gly 435 440 445 caa gtt ga Gin val 450 <210> 28 <211> 450 <212>蛋白質 <213>敗血性巴斯德桿菌 <220> <221> misc_特徵 <222> 125 <223> Xaa=任何或未知的胺基酸 <220> <221> niisC-特徵 <222> 133 <223> X⑽=任何或未知的胺基酸 <220> <221〉misc 特徵 <222> 141 ~ <223> Xaa=任何或未知的胺基酸 c220> <221> misc·特徵 <222> 151 <223> Xaa=任何或表知的胺基酸 <400> 28

Ser Thr Lys Val Gly Tyr Asp lie Asn Asn Thr His Arg Phe Thr Leu 1 5 10 15 136852·序列表.doc 62- 1105 1153 1201 1249 1297 1345 1353 1334884

Phe Leu Glu Asp Arg Arg Glu Lys Lys Leu Thr Glu Glu Lys Thr Leu 20 25 30

Gly Leu Ser Asp Ala Val Arg Phe Ala Asn Asp Gin Thr Pro Tyr Leu 35 40 45

Arg Tyr Gly lie Glu Tyr Arg Tyr Asn Gly Leu Ser Trp Leu Glu Thr 50 55 60

Val Lys Leu Phe Leu Ala T--ys Gin Lys lie Glu Gin Arg Ser Ala Leu 65 70 75 80

Gin Glu Phe Asp lie Asn Asn Arg Asn Lys Leu Asp Ser Thr Met Ser 85 90 95

Phe Val Tyr Leu Gin Arg Gin Asn lie Ala Arg Gly Glu Phe Ser Thr 100 105 110

Ser Pro Leu Tyr Trp Gly Pro Ser Arg His Arg Leu Xaa Ala Lys Phe 115 120 125

Glu Phe Arg Asp Xaa Phe Leu Glu Asn Met Asn Lys Xaa Phe Thr Phe 130 135 140

Arg Pro Trp Gin lie Asn Xaa Phe Arg Gin Gin Gly Arg Asn Asn Tyr 145 150 155 160

Thr Glu Val Phe Pro Val Lys Ser Arg Glu Phe Ser Phe Ser Leu Met 165 170 175

Asp Asp lie Lys lie Gly Glu Leu Leu His Leu Gly Leu Gly Gly Arg 180 185 190

Trp Asp His Tyr Asn Tyr Lys Pro Leu Leu Asn Ser Gin His Asn lie 195 200 205

Asn Arg Thr Gin Arg Leu Pro Tyr Pro Lys Thr Ser Ser Lys Phe Ser 210 215 220

Tyr Gin Leu Ser Leu Glu Tyr Gin Leu His Pro Ser His Gin lie Ala 225 230 235 240

Tyr Arg Leu Ser Thr Gly Phe Arg Val Pro Arg Val Glu Asp Leu Tyr 245 250 255

Phe Glu Asp Arg Gly Lys Ser Ser Ser Gin Phe Leu Pro Asn Pro Asp 260 265 270

Leu Gin Pro Glu Thr Ala Leu Asn His Glu lie Ser Tyr Arg Phe Gin 275 280 285

Asn Gin Tyr Ala His Phe Ser Val Gly Leu Phe Arg Thr Arg Tyr His 290 295 300

Asn Phe lie Gin Glu Arg Glu Met Thr Cys Asp Lys lie Pro Tyr Glu 305 310 315 320

Tyr Asn Arg Thr Tyr Gly Tyr Cys Thr His Asn Thr Tyr Val Met Phe 325 330 335

Val Asn Glu Pro Glu Ala Val lie Lys Gly Val Glu Val Ser Gly Ala 340 345 350 -63- 136852-序列表_此〇 isi 1334884

Leu Asn Gly Ser Ala Phe Gly Leu Ser Asp Gly Leu Thr Phe Arg Leu 355 360 365

Lys Gly Ser Tyr Ser Lys Gly Gin Asn His Asp Gly Asp Pro Leu Lys 370 375 380

Ser He Gin Pro Trp Thr Val Val Thr Gly He Asp Tyr Glu Thr Glu 385 390 395 400

Gly Trp Ser Val Ser Leu Ser C**.y Arg Tyr Ser Ala Ala Lys Lys Ala 405 410 4X5

Lys Asp Ala lie Glu Thr Glu Tyr Thr His Asp Lys Lys Val Val Lye 420 425 430

Gin Trp Pro His Leu Ser Pro Ser Tyr Phe Val Val Asp Phe Thr Gly 435 440 445

Gin Val 450

<210> 29 <211> 4936 <212> DNA <213>敗血性巴斯德样菌

<220> c221> CDS <222> (1078)..(2769) <220>

<223> hxuC

<400> 29 gtcaacaaca ccgatttaac catggctagc aacaacccac atcttttttc ggagatttca attgacatca aatgattgac atcagcccgt gcgtaagtgg aattccacca ggcatattgc acgatgcaca atccgagcaa aagcgcacag gcaaattaat attctagcaa cccgtagggc aagatctcat aggctttctc taaacccaac gagcgaccta ttttcaggcg agtaaccagt cagtttgtat acaacagata aacagcccac ccaatccaaa gcattacttc gccgtcaaca aaattagttg acggctgttt cttcattgag gactagtagc catgtaatga agttataaac gtaaattgcc tattaattaa gtccacaaat aacagtttaa ccggtcctaa gatagctcgg atgccacaca ttcatctttt aggaaaatag ctttttgaga ttttaaaaga aatgacacct gagttttttc ttgctctgcg aagtaaatga acctaaatct aataatgtgt atcggtgtga atttgttaat ggtttgatga catcatacag cctatcataa cggtcttgtt aattacgctt cgggcaatcg tgatcgatta ccatttttcc acgttaattt ctatgcttat tgatccgcca tcaatattta atgacttgat ttttctgcag tcagccaatt aaagtacgta 60 agcgtttcat 120 ttgcttaaaa 180 cttcatcttg 240 cattgcggta 300 agaaaccgtc 360 acgcggattt 420 tcgtcagcat 480 accaaatatc 540 ttgcggcttt 600 agacctcaac 660 ttgcaacatt 720 gaacacggct 780 -64- 136852-序列表.doc tsi 1334884 agaggagttt tcctctttca tccgtaacgc ccagctataa ttattggcaa aaagttgttc 900 aatctttttc attagagtga ttcctatacc gcaaaaataa gggggctagt atagcttaga 960 aatagacagt gggtaaagaa aggcaaaaaa ttgtatagga taacttgttt tttattgcca 1020 tttatttaga attagaatct ttaataataa aaataattat cattaaggtt aatagtt 1077 atg gat aaa aat tta atg aag gga tgt gta ttc tta tea ata gtc ggt 1125 Met Asp Lys Asn Leu Met Lys Gly Cys V^l Phe Leu Ser lie Val Gly 1 5 10 15 tgc ggt ate caa ata ggg eta gca tea aat cca aat cct cca gat gtg 1173 Cys Gly lie Gin lie Gly Leu Ala Ser Asn Pro Asn Pro Pro Asp Val 20 25 30 gat gag tta tta cct att att gtg aat get gat gaa gat aat aaa tta 1221 Asp Glu Leu Leu Pro lie lie Val Asn Ala Asp Glu Asp Asn Lys Leu 35 40 45

cca ggt cgt tet gta tta aaa cag aaa aat ate gat caa caa caa gca 1269 Pro Gly Arg Ser Val Leu Lys Gin Lys Asn lie Asp Gin Gin Gin Ala SO 55 60 gat aat gcc get gac tta ata aat att tta cct ggg gta aat atg geg 1317 Asp Asn Ala Ala Asp Leu lie Asn lie Leu Pro Gly Val Asn Met Ala 65 70 75 80 gga gga ttt ege cct ggt ggt caa aca tta aat att aat gga atg ggt 1365 Gly Gly Phe Arg Pro Gly Gly Gin Thr Leu Asn lie Asn Gly Met Gly 85 90 95 gat get gaa gat gtt aga gtt caa eta gac ggc gca aca aaa agt ttc 1413 Asp Ala Glu Αερ Val Arg Val Gin Leu Asp Gly Ala Thr Lys Ser Phe 100 105 1X0 gaa aaa tat caa caa ggc tet att ttt att gaa cct gag tta tta aga 1461 Glu Lys Tyr Gin Gin Gly Ser lie Phe lie Glu Pro Glu Leu Leu Arg 115 120 125

aag gtg aca gta gac aaa gga aat tat tet cct caa tat ggc aat ggt 1509 Lys Val Thr Val Asp Lys Gly Asn Tyr Ser Pro Gin Tyr Gly Asn Gly 130 135 140 ggc ttt get ggt act gta aaa ttt gaa aca aaa gat gca act gat ttt 1557 Gly Phe Ala Gly Thr Val Lys Phe Glu Thr Lys Asp Ala Thr Asp Phe 145 150 155 160 ttg aaa gaa aat cag aaa ata ggt gga tta ttt aaa tat gga aat aat . 1605 Leu Lys Glu Asn Gin Lys lie Gly Gly Leu Phe Lys Tyr Gly Asn Asn 165 170 175 age aat aat aac caa aaa act tat agt aca gcc eta gtt tta cag aat 1653 Ser Asn Asn Asn Gin Lys Thr Tyr Ser Thr Ala Leu Val Leu Gin Asn 180 185 190 gaa caa aaa aat att gat ttg tta tta ttt ggt tet gta aga aat gca 1701 Glu Gin Lys Asn lie Asp Leu Leu Leu Phe Gly Ser Val Arg Asn Ala 195 200 205 age aat cat aca aga cct gat aaa agt aaa att ett ttt tea aaa aac 1749 Ser Asn Tyr Thr Arg Pro Asp Lys Ser Lys lie Leu Phe Ser Lys Asn 210 215 220 •65· 136852·序列表.doc

I SI 1334884

aat caa aaa agt gga tta aca aaa gta aat tgg caa att act cct gaa 1797 Asn 心S Gin Lys Ser Gly Leu 230 lie Lys Val Asn Trp 235 Gin lie Thr Pro Glu 240 cat tta tea act tta tcc agt gtt tat ggc att cat aaa ggg tgg gaa 1845 His Leu Leu Thr Leu 245 Ser Ser Val Tyr Gly 250 lie His hys Gly Trp Glu 255 cct tgg gca gca aaa aga gat gtg atg teg aga cca aca gaa aca gaa 1893 Pro Trp Ala Ala 260 Lys Arg Asp Val Met 265 Ser Arq Pro Thr Glu 270 Thr Olu ata aaa cac tat ggg att gat gtt geg tgg aaa cgt aaa Ctt gtt tat 1941 lie Lys His 275 Tyr Gly lie Asp Val 280 Ala Trp Lys Arg Lys 285 Leu Val Tyr cga gat caa aaa gat gaa agt tat tea ttg aaa tat ege tat tta cct 1989 Arg Asp 290 Gin Lys Asp Glu Ser Tyr 295 Ser Leu Lys Tyr 300 Arg Tyr Leu Pro gaa aat aat aag tgg att aat ttg tet gtt cag ctg agt tat agt aaa 2037 Glu 305 Asn Asn Lys Trp He 310 Asn Leu Ser Val Gin 315 hen Ser Tyr Ser hys 320 aca gag cag aat gat act ege cat gag aaa gtc act tet tea ttc eta 2085 Thr Glu Gin Asn Asp Thr 325 Arg His Glu Lys 330 Val Thr Ser Ser Phe 335 Leu ggt aca tta gga aat aaa agt tgg ata act tat tea gat ctt act ttt 2133 Gly Thr Leu Gly Asn 340 Lys Ser Trp lie 345 Thr Tyr Ser Asp Leu 350 Thr Phe gat ata agt aac aca agt act eta aat att ggg cgt get gag cat gaa 2181 Asp lie Ser 355 Asn Thr Ser Thr lieu 360 Asn lie Gly Arg Ala 365 Glu His Glu eta eta ttt ggt tta cag tgg tta aaa aat aaa aga aat acc ctt atg 2229 Leu Leu 370 Phe Gly Leu Gin Trp 375 Leu Lys Asn Lys Arg Asn 360 Thr Leu Met tat cat aaa ggg gga gtc aag aag gca gac tat aat tat ggc tat ttt 2277 Tyr His 385 Lys Gly Gly Val 390 Lys Lys Ala Asp Tyr Asn 395 Tyr Gly Tyr Phe 400 cag cct tat tat atg cct tet gga ege cag tat aca caa gca ttt tat 2325 Gin Pro Tyr Tyr Met 405 Pro Ser Gly Arg Gin Tyr 410 Thr Gin Ala Phe 415 Tyr tta caa gat caa ata aaa tgg cag aat ttc etc ttt aca gga ggg ata 2373 Leu Gin Asp Gin 420 lie Lys Trp Gin Asn 425 Phe Leu Phe Thr Gly Gly 430 lie aga tat gac cat ate aat aac ata ggg cag aaa aat tta geg cca cga 2421 Arg Tyr Asp 435 His lie Asn Asn lie 440 Gly Gin Lys Asn Leu 445 Ala Pro Arg tat aat gat ate tet gca gga cat gat tat age cag aaa aat tat aat 2469 Tyr Asn 450 Asp lie Ser Ala Gly His 45S Asp Tyr Ser Gin 460 Lys Asn Tyr Asn ggt tgg tet cat tat tta ggt ett aag tat gat gta aat cat tat tta 25X7 Gly 465 Trp Ser Tyr Tyr Leu 470 Gly Leu Lys Tyr Asp 475 Val Asn His Tyr Leu 480 136852-序列表.doc 1334884 agt tta ttt acg aat ttt agt aaa act tgg cga gcc cct gtt att gat 2565

Ser Leu Phe Thr Asn Phe Ser Lys Thr Trp Arq Ala Pro Val lie Asp 485 490 495 gaa cag tat gag aca caa tat agt caa get tet gta tet geg act tet 2613

Glu Gin Ύγτ Glu Thr Gin Tyr Ser Gin Ala Ser Val Ser Ala Thr Ser 500 505 510 tta aat tta gaa aaa gaa atg att aat caa acc aga gtg ggt gga att 2661

Leu Asn Leu Glu Lys Glu Met lie Asn Gin Thr Arg Val Gly Gly He 5X5 520 525 att act etc aat cat eta ttt cag gaa aat gat get ttt caa ttt aga 2709 lie Thr Leu Asn His Leu Phe Gin Glu Asn Asp Ala Phe Gin Phe Arg 530 535 540 act act tat ttt tac aat ege ggc aag aat gaa ate ttc aaa acg aga 2757

Thr Thr Tyr Phe Tyr Asn Arg Gly Lys Asn Glu lie Phe Lys Thr Arg 545 550 555 560

ggg gtt aac cgt tagagttggt tgaaatgact gaaaaattag acctatacgt 2809

Gly Val Asn Arg tactgttaaa ggtggcggta tttctggtca agcgggtgca atccgtcacg gtatcactcg 2869 tgeattaate gaatatgatg agagtttacg ctctgtatta cgcgcagctg gtttcgttac 2929 tegegatgea cgtcaagttg aacgtaaaaa agtgggttta cgcaaagcgc gtcgtcgtcc 2989 acaattctca aaacgttaat ttttctttta cgttttatat teagattgea agcccaaaag 3049 gcttgcaatt tttttatctc aataaaattt aegataatet ttggaaatca gtgggcgatt 3109 tgtggtagaa taaacgccca ttttttatat aaaatcatgc cagaatcagg caaagtttaa 3169 taaattttaa ttcattttag agctgtcgga ggaatagatg acaagcgctg caaataaacg 3229 ttcaataatg acactttttt cagataaaac agatatttat tgccaccaag taaggattgt 3289 tttggctgaa aagggtgttg cttatgaaac ggaagttgta gatcctcaag tcgtatcaga 3349 agatttaatg gaattaaatc cgtatggcac gttgccgaca ttagttgatc gtgatttagt 3409

gttatttaat teaegtatta ttatggaata tettgatgag cgtttccctc atccaccttt 3469 gatgcctgtt tatccagtgg cacgtgggaa aagccgttta ttaatgttac gtattgagea 3529 gtgctgtcgc 3589 agatgeetta 3649 tatggegtat 3709 cttacatggc 3769 cgaaaaactt 3829 ttacttgtta 3889 ggtggacgcg 3949 agattggtac attaaaacaa ttttatgagc gcaagaactg acgtgttttt aatggatgag agagegtatt acttatgttg ccagtattag ttaagagaag gaagagttta ggtgtggatt gaaegegatt aaataatcag atgattggtt gtgtgaaagt caaaagctga agattttagc gtttagtaga tcagtggggc catttatgca tatgctacat agtggataat ccctgtggaa aaaaggcacg gattgcgcct ttgttatatc gggtagcaaa atetttagge aaatcatcac gatttcaccc tatgtcaaag gacgcagaac attttcacgc gccccactat gcaattaaag gtgtcggctc caaagcgtcc cttatttagt atgggcaaat • 67- 136852·序列表.doc i si 1334884

ttatccgcga atgcgacagg taatctggca ctaagcaatg aaagtattca gtttagcgcg 4069 cgtcttcgtg gtatttcaca agacacctcc actcctacgg gggccgcgct agccatctat 4129 gctcgcgaaa atggtgatgg tgtactgttt gaacctgaag cgacttatga tgagctcgca 4189 acacaaaata ctggtatcga gcagccactg agctttgttg aggctgtcga taaaccaaaa 4249 accagcgaga atacccaaaa aagcacaaac aaagacaaaa cgacggaaaa aaaagcgact 4309 tctcatttaa gaattaccaa ataaaagagg ttttctttct Ccataaaaaa acacgctttt 4369 acgcgtgttt ttttgttgcg gacagtttat tgtgccattt tttttgcggc ttttaagaag 4429 ccttgcgcac tcgtgtagat gtcactttta ttctgtgccg ctaaaatcat atccgacatt 4489 tcacgaaagg ccccttgtcc acctttcagg cttagtacgt gatcagcgtg cattttgata 4549 taagcggcgc atcttgtact gcaaaggcaa caccacagcg gcaaaagcag gcagatcaac 4609 gctgtcatca ccaatataag ccgtttcttg cgcacagaca ttggcttgtt gtatcaattc 4669 aagacaggcg ctttcttttt ccaatttgcc gaggaaaaag tgttggatgc ctagatctgc 4729 aatacgtttg cgtagaatcg gggaatctcg ccccgagagt accgcgactt gaatgccaga 4789 ttccattaac attctgatcc ccaagccatc acgaacatga aaggttttga aagcttcacc 4849 atgggcatcg taatgcaaag agccgtcggt cagtacaccg tcgatatctg taatcacaaa 4909 tttaattttt ttgagttttt ccgttga 4936 <210> 30 <21X> 564 <212>蛋白質 <213>敗血性巴斯德样菌

<400> 30 Met Asp Lys 1 Cys Gly lie

Asp Glu Leu 35 Pro Gly Arg 50 Asp Asn Ala 65 Gly Gly Phe Asp Ala Glu

Asn Leu Met Lys Gly Cys Val Phe Leu Ser lie Val Gly 5 10 15 Gin lie Gly Leu Ala Ser Asn Pro Asn Pro Pro Asp Val 20 25 30 Leu Pro lie lie Val Asn Ala Asp Glu Asp Asn Lys Leu 40 45 Ser Val Leu Lys Gin Lys Asn lie Asp Gin Gin Gin Ala 55 60 Ala Asp Leu lie Asn lie Leu Pro Gly Val Asn Met Ala 70 75 80 Arg Pro Gly Gly Gin Thr Leu Asn lie Asn Gly Met Gly 85 90 95 Asp Val Arg Val Gin Leu Asp Gly Ala Thr Lys Ser Phe 100 105 110

Glu Lys Tyr Gin Gin Gly Ser lie Phe lie Glu Pro Ulu Leu Leu Arg 115 120 125 136852·序列表.doc -68- tsi 1334884

Lys Vai Xftr Val Asp Lys Giy Asn lyr Ser Pro Gin Tyr Gly Asn Gly 130 135 140

Gly Phe Ala Gly Thr Val Lys Phe Glu Thr Lys Asp Ala Thr Asp Phe 145 150 155 160

Leu Lys Glu Asn Gin Lys lie CJly Gly Leu Pfte Lys Tyr Gly Asn Asn 16S 170 175

Ser Asn Asn Asn Gin Lys Thr Tyr Ser Thr Ala Leu Val Leu Gin Asn 180 165 190

Glu Gin Lys Asn lie Asp Leu Leu Leu Phe Gly Ser Val Arg Asn Ala 195 200 205

Ser Asn Tyr Thr Arg Pro Asp Lys Ser Lys lie Leu Phe Ser Lys Asn 210 215 220

Asn Gin Lys Ser Gly Leu lie Lys Val Asn Trp Gin lie Thr Pro Glu

225 230 235 240

His Leu Leu Thr Leu Ser Ser Val Tyr Gly lie His Lys Gly Trp Glu 245 250 255

Pro Trp Ala Ala hys Arg Asp Val Met Ser Arg Pro Thr Glu Thr Glu 260 265 270 lie Lys His Tyr Gly lie Asp Val Ala Trp Lys Arg Lys Leu Val Tyr 275 280 285

Arg Asp Gin I»ys Asp Glu Ser Tyr Ser Leu Lys Tyr Arg Tyr Leu Pro 290 295 300

Glu Asn Asn Lys Trp lie Asn Leu Ser Val Gin Leu Ser Tyr Ser Lys 305 310 315 320

Thr Glu Gin Asn Asp Thr Arg His Glu Lys Val Thr Ser Ser Phe Leu 325 330 335

Gly Thr Leu Gly Asn Lys Ser Trp lie Thr Tyr Ser Asp Leu Thr Phe 340 345 350

Asp lie Ser Asn Thr Ser Thr Leu Asn lie Gly Arg Ala Glu His Glu 355 360 365

Leu Leu Phe Gly Leu Gin Trp Leu Lys Asn Lys Arg Asn Thr Leu Met 370 375 380

Tyr His Lys Gly Gly Val Lys Lys Ala Asp Tyr Asn Tyr Gly Tyr Phe 385 390 395 400

Gin Pro Tyr Tyr Met Pro Ser Gly Arg Gin Tyr Thr Gin Ala Phe Tyr 405 410 415

Leu Gin Asp Gin lie Lys Trp Gin Asn Phe Leu Phe Thr Gly Gly lie 420 425 430

Arg Tyr Asp His lie Asn Asn lie Gly Gin Lys Asn Leu Ala Pro Arg 435 440 445

Tyr Asn Asp lie Ser Ala Gly His Asp Tyr Ser Gin Lys Asn Tyr Asn 450 455 460 69- 136852·序列表.doc IS! 1334884 uiy _irp Ser Tyr Tyr Leu G丄y Leu Lys Tyr Asp Val Asn His Tyr Leu 465 470 475 480

Ser Leu Phe Thr Asn Phe Ser Lys Thr Trp Arg Ala Pro Val lie Asp 485 490 495

Giu Oln lYr Glu Thr Gin Tyr Ser Gin Ala Ser Val Ser Ala Thr Ser 500 505 5X0

Leu Asn Leu Glu Lys Glu Met lie Asn Gin Thr Arg Val Gly Gly lie 515 520 525

He Thr Leu Asn His Leu Phe Gin Glu Asn Asp Λίβ Phe Gin Phe Arg 530 535 540

Thr Thr Tyr Phe Tyr Asn Arg Gly Lys Asn Glu lie Phe Lys Thr Arg 545 550 555 560

Gly Val Asn Arg

<210> 31 <211> 9814 <212> DNA <213>敗血性巴斯德样菌 <220>

<221> CDS <222> (4762) . . (7662) <220> <223> iroA <400> 31 gtcgacctgc agcagacaat gtccactgtg taatagtcca acgactgtta taaaaatctt 60 ccgctgcttt ttctaatgtt tgtacacggt gtttttcaaa ttcttccaaa ttcttggttg 120 gttggactaa aagttgttca gccgccgctt taaaattggc gacaaaatcc gattgtttga 180 ttaaaccata ggattttgct tggatttgtg cgccggctgc ttcacccaat ttatcgaaaa 240

attcaggcgt ataagCcacg tttgggatcg taggcgcgat gatctgatta acaccagcct 300 gttgtaatgc tccccattgc tgtgccatcg tttgtgccat ggttttaata ctggctaaca 360 cgtaggcttg tttctcttca ggtgttgtct tcgttactgc tgttgctaat actgtcgcca 420 aatcattacc acctgcccat agaatatgta aagcttcttt tttgactggc gcatgtaaat 480 actcgttgat ctgcttttct agtgctaaat gaggttgttc tgctgttcta gtattatgtg 540 caccaacaat caccccaccg ctgtacgcgt agttcaaacc accttgtgta gaaggtatta 600 attttttccc aaatgcttga gctaaatatt catcataaag gtgatgatag tttccatcag 660 cctttaaata agaggctttc ttattccaac ctgtttgccc catatcactt aaactatcac 720 caaacacgac aacaccttgt gccagagcag ccgaacttag tgaacaaaat aatgcgaatg 780 aaagtgtatt aattttcaca ataatgtcct tcaattattc atcgtcagtc aaaaatttgc 840 -70- 136852·序列表.doc S3 1334884 gtcatcatac gttgattata ggaagatacc tagccagacc actactggta tgaacagaag 900 tcaatgtcta atcacataaa aaagcctctg tgctttcaca cagaggcttt tatgtccatt 960 cacctactca aattacatcg cttgagtaaa ggtacgcgta atcacgtctt gttgttgttc 1020 tttggttaac gagttaaagc gtactgcata acccgaaaca cgaatggtta attgtggata 1080 tttctccgga ttttccattg catctaacaa catttcacgg ttcatgacgt tcacgtttaa 1140 gtgttgtccg ccttcaatcg ttgcttcatg gtggaagtaa ccgtccatta gacctgcaag 1200 gttacgtttt tgtgcttcgt aatctttacc taaggcattt ggtacgattg agaaggtata 1260 agaaatacca tctttcgcat aagcaaatgg caatttcgca acagaagtta acgatgctac 1320 tgcacctttt tggtcacgac cgtgcattgg gttcgcaccc ggtccaaatg gtgcaccaga 1380 acgacgacca tctggggtgt tacctgtttt cttaccataa accacgttag atgtaatagt.1440

aagtacagat tgtgtcggtg tcgcattacg ataagtaccc aatctttgga ttttcttcat 1500 aaagcgttca actaaatcac aagcgatttc atcaacacgg ttatcgttgt taccgaattg 1560 tggatattcg ccttcaattt caaagtcgat tgccacatct cttgcaatac caaccacttc 1620 acccgcttta tttttgattt cgatatcacc acgaactggt ttcactttcg catatttaat 1680 ggcagataaa gagtccgctg cgacagaaag ccctgcgata ccacaagcca tagtacggaa 1740 tacatcacga tcatgaagtg ccattagtgc ggcttcatac gcgtatttat cgtgcataaa 1Θ00 gtgaatgatg ttcaatgcag taacatattg tttcgctaac caatccataa agctgtctaa 1860 gcgcgtcatg acatcatcat aatctaagta ttcgctggta attggatcgg ttttcggtcc 1920 tacttggtca cctgatttct catccacacc gccattgatt gcgtataaca aggttttcgc 1980· taagttggca cgtgcaccga agaattgcat cattttaccc acgatcattg gcgatacaca 2040 gcatgcaatc gcatagtcat cgttttggaa gtcaggacgc attaagtcat cgttttcata 2100 ctgaacagaa gacgtatcga tagacacttt tgccgcataa cgtttgaaac cttctggtaa 2X60

tttttcagac caaagaatcg ttaagtttgg ctctggtgac ggtcccatcg tacaaagggt 2220 gtgtaagata cggaagctgt ttttggttac taaagtacga ccatctaagc ccatacccgc 2280 taaggtttcc gttgcccaca ttgggtcgcc cgagaataat tgatcgtact ctggggtacg 2340 taagaaacgc accatacgta atttcatgac taagtggtca attaattctt gtgcttcttg 2400 ttctgtaatt ttgcctgctt ttaaatcacg ctcaatataa atatctaaga aagtegatac 2460 acgaccaaat gacatggcag caccgttttg tgatttcact gcagcggtac gactttgcac 2520 agttatcgga agataattga aatcataacc agcaacaaat gtcagccgtt ctatttcttc 2580 tgaagaatat cggcgattgc gttctggcgg aatggctggt tttttcacat tcttgagatg 2640 attttctttc aagtatttcc attctgtgat tgctgtcgta ataatgtgcg aaagagtatt 2700 ccattctcta agcacactcg ccgcactaac ttgtgaaagt cgttcatcac gccactgcac 2760 •71 · 136852-序列表.doc ISi 1334884 aaagtgatgt tcttgtaaat cgattaatct cacgttagaa atcggcatat ttagcaagcg 2β20 aagtaaacgc aacttttcag cacgatagcc ctttttattt atgctcactt ctttgatata 2880 tttatcgaca agttgtgcaa aagtgatgtc gggaatatca gtgaatacac cgtttatgat 2940 tttttgttct atgtctaacg cccacgcttg cgcatcagct ttagtccgaa atgttgctga 3000 tttatagaca ccttttttgc gaatttgaac tcgccaacct gatttttgtt tgttaaaagt 3060

cgccacgcct aactaccatt aattttgcac aattcgttgt gcaattttgt gcaattttat 3120 ttcttaaatg atgtttagcg gatcataaat gaagaaaaaa cagaagtaaa acactatata 3180 aaaaaacagt atattttttg ttgaagtgct taatgtagtt tgtaacttat tgatttttaa 3240 atattaaaga taaagaaaaa gccgacaaaa gtcggctttc tttttttatg ctgtttatca 3300 atggtgccta gggccggact cgaaccggca cacccgaagg cggttgattt tgaatcaact 3360 gcgtctacca atttcgccac ccaggctaat attgataaag agcatcaaat taggtctgta 3420 ttataccttt tcattgatag gttacaagca aaaaatgatt ttagatattc aattgtcttt 3480 attttcatca gtgtggctgt tctctcttac tttattcagg tgcagtgaag taaccttata 3540 aaaataaagg ttttttgttt aaattttaaa taactttttt gtaaaaagat cgcagtttct 3600 tttgaaaaaa agaccgatta caaatataat taaaaactat tatcaatatt gataataatg 3660

ggattttaac agattatatt acctgaaggt acgtgcggag aaaagaagga ttattataat taacatcagc cgcatttatt gcgtcaatta agtaatgaca gttaaaggca tgtgagttat tgcacgtcat tgaaagttaa tgttgtcgag tggtgcatta ctcacaaggg catgacattt acacaaaatc gaaattttat gaagtgcttg tcgatttttg ttgaatatga aaaattgcct gcgaataatg tttccagaac acaaatcgtc caatgtaaaa gcgccatttg gcacgtaatc agtcgtcaga agagaaagtg atggatgaat ttatttgtaa ataaggataa cactggcaat gtgctttacc ctgagatcag aaattaaaga aagatgaaga ttgtgagttt ggcaaactat aagttcaaaa aagaagtatt cagtcatgct caattaataa tacaagaagt tttttgctcg aagaatggca tgtctcggat aaggttttgg agtcaagatg tactttggat tgatgagttt tagttggggg ccgttttccg aggcacgctt accgtttcgc ttttaaagtt atttaaagca acaaaatcgt cgctactgtc cggggaatac accaaaagtt tcgtcgttta ttctggtgtt gaaagatttt tcaagctttc atgctaaaag atggcttcgc gttcgagtgt attttcacga accgggatgg catggtcacc ggtaaagaaa tatttggggc tttatttaga ttaggtccag ggtgaagatg caagtcttta tctttaatgt tcttttgatg gaggcactca catttgttta caagtgagta cacaaattgc 3720 actttggcaa 3780 ttcctgcgga 3840 ccattattga 3900 ttgggcgtgg 3960 ttaaattaga 4020 gttataacat 4080 gcgatgctga 4140 aggttaaaaa 4200 aaattcaaga 4260 gtaatcccaa 4320 tttctactat 4380 tgatcgaaga 4440 cggttgcgcg 4500 aagcaagaca 4560 gttttaagcc 4620 -72- 136852-序列表.doc is] 1334884

agtgagcttt gttcacttgg ttgaaggaca ccaagaataa attttgtcaa ctttttaggg 4740 catctactcg aggttaggaa a aeg cgc aca aca aca ata aaa ttt tet gca 479X Met Arg Thr Thr Thr lie Lys Phe Ser Ala 1 5 10 att aca ttg gca tta ttg agt tat tgt ggg acc att ttg gcg gat agt 4839 lie Thr Leu Ala Leu Leu Ser Tyr Cys Gly Thr lie Leu Ala Asp Ser 15 20 25 cat caa gag gcg act gaa ett gat aeg ate acc gtt tet tet caa caa 4887 His Gin Glu Ala Thr Glu Leu Asp Thr lie Thr Val Ser Ser Gin Gin 30 35 40 gat gag atg aat att aaa gag aaa aaa ate ggt gaa act gtg aaa aeg 4935 Asp Glu Met Asn lie Lys Glu Lys Lys He Gly Glu Thr Val Lys Thr 45 50 55 gcg agt caa ttg aaa cgc cag caa gta cag gat agt cgt gat ett gtg 4983 Ala Ser Gin Leu Lys Arg Gin Gin Val Gin Asp Ser Arg Asp Leu Val 60 65 70 cgc tat gaa acc ggt gtg act gtg gta gaa get gga cgt ttt ggt teg 5031 Arg Tyr Glu Thr Gly Val Thr Val Val Glu Ala Gly Arg Phe Gly Ser 75 80 65 90 age ggt tat gec att cgt ggt gtg gat gag aac ega gta gca att aca 5079 Ser Gly Tyr Ala lie Arg Gly Val Asp Glu Asn Arg Val Ala lie Thr 95 100 105 gta gat ggc tta cat caa gca gaa acc ett tet tet caa ggc ttt aaa 5127 Val Asp Gly Leu His Gin Ala Glu Thr Leu Ser Ser Gin Gly Phe Lys 110 115 120 gaa tta ttc gaa 99t tac ggc aat ttt aac aat acc ega aat agt gtg 5175 Glu Leu Phe Glu Gly Tyr Gly Asn Phe Asn Asn Thr Arg Asn Ser Val 125 130 135 gaa att gag aeg ttg aaa gtc get aaa ate gcg aaa ggt get gat tet 5223 Glu He Glu Thr Leu Lys Val Ala Lys lie Ala Lys Gly Ala Asp Ser 140 145 150 gta aaa gtg ggt agt ggt tet ttg gga ggc get gta ett ttt gaa aca 5271 Val Lys Val Gly Ser Gly Ser Leu Gly Gly Ala Val Leu Phe Glu Thr 155 160 16S 170 aaa gat gec aga gat ttc ctg act gaa aaa gat tgg cat ate ggc tat 5319 Lys Asp Ala Arg Asp Phe Leu Thr Glu Lys Asp Trp His He Gly Tyr 175 180 185 aaa gcg ggc tac tea aeg gca gat aat cag gga tta aat gca gtg act 5367 Lys Ala Gly Tyr Ser Thr Ala Asp Asn Gin Gly Leu Asn Ala Val Thr 190 195 200 ett gca ggt cgc tat caa atg ttt gat gca tta att atg cat tet aag 5415 Leu Ala Gly Arg Tyr Gin Met Phe Asp Aid Leu lie Met His Ser Lys 205 210 215 ega cat gga cat gaa tta gaa aat tat gac taz aaa aat ggc aga gat 5463 Arg His Gly His Glu Leu Glu Asn Tyr Asp Tyr Lys Asn Gly Arg Asp 220 225 230 136852-序列表.doc -73-

1 SI 1334884

act caa ggg aaa gaa aga gag aaa geg gat cct tat aeg att aeg aaa 5511 lie Gin Gly Lys Glu Arg ^4U Glu Lys Ala Asp Pro 245 Tyr Thr lie Thr Lys 250 gaa agt aca tta gtg aaa ttc cct ttt teg cca aca gaa aat cat cgt 5559 Glu Ser Thr Leu Val 255 Lys Phe Ser Phe Ser 260 Pro Thr Glu Asn His 265 Arg ttt aca gtc get cct gat act tat ctt cag cat tcc ege gga cat gat 5607 Phe Thr Val Ala 270 Ser Asp Thr Tyr Leu 275 Gin His Ser Arg Gly His 280 Asp ctt tea tat aat ctt 9tt gca aca aca cat att cag tta gat gag aaa 5655 Leu Ser Tyr 28S Asn Leu Val Ala Thr 290 Thr His lie Gin Leu 295 Asp Glu Lys gaa tet cgt cat gca aat gat ttg aca aaa cgt aaa aat gtt tec ttt 5703 Glu Ser 300 Arg His Ala Asn Asp 305 Leu Thr Lys Arg Lys Asn 310 Val Ser Phe act tat gaa aat tat act gtt aeg cca ttt tgg gat aeg etc aag tta 5751 Thr 315 Tyr Glu Asn Tyr Thr 320 Val Thr Pro Phe Trp Asp Thr 325 Leu Lys Leu 330 age tat tea caa caa aga att aca aca aga gca aga aca gaa gat tat 5799 Ser Tyr Ser Gin Gin 335 Arg lie Thr Thr Arg Ala 340 Arg Thr Glu Asp 345 Tyr tgt gat gga aat gag tta tgt gat tet tat aaa aat cca tta ggt etc 5847 Cys Asp Gly Asn 350 Glu Leu Cys Asp Ser 355 Tyr Lys Asn Pro Leu 360 Gly Leu caa ttt aaa gat ggt cag att ctt gat cct gca 999 aat aaa att aaa 5895 Gin Phe Lys 365 Asp Gly Gin lie Leu 370 Asp Pro Ala Gly Asn 375 Lys lie Lys eta cag ggg tet gga ttg age act cag att gta gat gaa aat gga aaa 5943 Leu Gin Gly 380 Ser Gly Leu Ser 385 Thr Gin lie Val Asp 390 Glu Asn Gly Lys cca ttt cct aca aca aca ggt act aat aat get get ttt agt aat aat 5991 Pro 395 Phe Pro Thr Thr Thr 400 Gly Thr Asn Asn Ala 405 Ala Phe Ser Asn Asn 410 tta ega etc agg cct aca ggt ttt tgg tta gat tgc teg gtt ttt gac 6039 Leu Arg Leu Arg Pro 415 Thr Gly Phe Trp Leu 420 Asp Cys Ser Val Phe 425 Asp tgt aat aaa cca ttc act gtt tat aat att agt aat gga aca tat caa 6087 Cys Asn Lys Pro 430 Phe Thr Val Tyr Asn 435 lie Ser Asn Gly Thr 440 Tyr Gin gca aga gag gtt eta ctg tet gaa gag ata act gtg gat ggt aaa tta 6135 Ala Arg Glu 445 Val Leu Leu Ser Glu 450 Glu lie Thr Val Asp Gly 455 Lys Leu cac aaa aca get aag gaa gaa gga ggg etc cca aat tat ttg att tta 6183 Tyr Lys 460 Thr Ma Lys Glu Glu 465 Gly Gly Leu Pro Asn Tyr 470 Leu lie Leu cct aat tet aaa ggc tac ctt cct tat gat tat aaa gaa agg gat ctt 6231 Pro 475 Asn Ser Lys Gly Tyr 48u Leu Pro Tyr Asp Tyr 485 Lys Glu Arg Asp Leu 490 136852·序列表.doc • 74- IS1 1334884 aat acg aat aca aaa caa att aat tta gat tta act aaa aca ttt tta 6279

Asn Thr Asn Thr Lys Gin lie Asn Leu Asp Leu Thr Lys Thr Phe Leu 495 500 505 act ttc aac ate gaa aat aat ctg tea tat ggt gga gtt tat tet egg 6327

Thr Phe Asn He Glu Asn Asn Leu Ser Tyr Gly Gly Val Tyr Ser Arg 510 515 520 ata gag aaa gaa atg att aat aaa get ggt tat gag ggg aga aat cct 6375 lie Glu Lys Glu Met lie Asn Lys Ala Gly Tyr Glu Gly Arg Asn Pro 525 530 535 act tgg tgg get gat aga att tta ggg caa agt agt tac tgt ggt tat 6423

Thr Trp Trp Ala Asp Arg He Leu Gly Gin Ser Ser Tyr Cys Gly Tyr 540 545 550 aat gca ttg aag tgt ccg aaa cat gag cca tta acg tea ttt tta att 6471

Asn Ala Leu Lys Cys Pro Lys His Glu Pro Leu Thr Ser Phe Leu lie 555 560 565 570

cca gtt gaa gca acg aca cag tea tta tat ttt gca aat att ett aag 6519

Pro Val Glu Ala Thr Thr Gin Ser Leu Tyr Phe Ala Asn lie Leu Lys 575 580 585 gta cat aat atg att age ata gat tta gga tat cgt tat gat cat att 6567

Val His Asn Met lie Ser lie Asp Leu Gly Tyr Arg Tyr Asp His lie 590 595 600 aaa tat aac cct gaa tac act cca gga gta act cca aaa att cca gat 6615

Lys Tyr Asn Pro Glu Tyr Thr Pro Gly Val Thr Pro Lys lie Pro Asp 605 610 615 gat atg gta aaa ggt ttg ttt att cct atg cca aaa gag cca cag eta 6663

Asp Met Val Lys Gly Leu Phe lie Pro Met Pro Lys Glu Pro Gin Leu 620 625 630 aag gat ttt gat tat aac tat get aaa ttt ggt gag gcc Cat aaa aaa 6711

Lys Asp Phe Asp Tyr Asn Tyr Ala Lys Phe Gly Glu Ala Tyr Lys Lys 635 640 645 650 tgg aaa gaa tat ctg cca aaa aat geg gaa gaa aat att get tac att 6759

Trp Lys Glu Tyr Leu Pro Lys Asn Ala Glu Glu Asn lie Ala Tyr lie

655 660 665 get caa gat aag aca ttt aaa aaa cat tet tat tet ett ggt gca act 6807

Ala Gin Asp Lys Thr Phe Lys Lys His Ser Tyr Ser Leu Gly Ala Thr 670 675 680 ttc gat cct ctg aat ttt tta ega gta caa gta aaa tat tea aaa ggg. 6855

Phe Asp Pro Leu Asn Phe Leu Arg Val Gin Val Lys Tyr Ser Lys Gly 685 690 695 ttt aga gcc ccg act teg gat gaa ett tat ttt acc ttt aag cat cca 6903

Phe Arg Ala Pro Thr Ser Asp Glu Leu Tyr Phe Thr Phe Lys His Pro 700 705 710 gat ttt acg att tta ccg aac ccc gtg ttg aaa cca gag gaa gca aaa 6951

Asp Phe Thr lie Leu Pro Asn Pro Val Leu Lys Pro Glu Glu Ala Lys 715 720 725 730 aat caa gag att gca tta aca gtg cac gat aat tgg gga ttt gtt age 6999

Asn Gin Glu lie Ala Leu Thr Val His Asp Asn Trp Gly Phe Val Ser 735 740 * 745 -75- 136852-序列表.doc 1334884

aca agt gtt ttc caa aca aag tat cgt cat ttt att gat tta gcg tat

Thr Ser Val Phe Gin Thr Lvs Tyr Arg His Phe lie Asp Leu Ala Tvr 750 ' 755 760 tta ggt tea aga aat tta teg aat tcc gtg gga ggg cag gca caa gca

Leu Gly Ser Arg Asn Leu Ser Asn Ser Val Gly Glv Gin Ala Gin Ala 765 77〇 775 aga gat ttc caa gtt tat caa aat gtc aat gtc gat aat gcc aaa gtt

Arg Asp Phe Gin Val Tyr Gin Asn Val Asn Val Asp Asn Ala Lys Val 780 785 790 aaa gga ett gaa att aat gca cgt ttg aat ttg gga tat ttt tgg cat

Lys Gly Leu Glu lie Asn Ala Arg Leu Asn Leu Gly Tyr Phe Trp His 795 800 80S 810 gtg ttg gat gga ttt aat aeg age tat aaa ttc act tac caa cgt ggt

Val Leu Asp Gly Phe Asn Thr Ser Tyr Lye Phe Thr Tyr Gin Arg Gly 815 820 825 cgt ttg gat ggc gat cgt cca atg aat gcg att cag cct aaa get tet

Arg Leu Asp Gly Asp Arg Pro Met Asn Ala lie Gin Pro Lys Ala Ser Θ30 835 840 gtt ttt ggt ttg ggc tac gat cat aaa gaa aat aaa ttt ggc get gat

Val Phe Gly Leu Gly Tyr Asp His Lys Glu Asn Lys Phe Gly Ala Asp 845 850 855 tta tat att aca cgt gtg agt gag aaa aaa gcg aaa gac act tat aat

Leu Tyr lie Thr Arg Val Ser Glu Lys Lys Ala Lys Asp Thr Tyr Asn 860 865 870 atg ttc tat aaa gaa cag gga tat aaa gat agt get gtt cgt tgg aga

Met Phe Tyr Lys Glu Gin Gly Tyr Lys Asp Ser Ala Val Arg Trp Arg Θ75 880 885 890 agt gat gac tat aeg eta gtt gat gcg gtt ggt tat att aaa ccg att

Ser Asp Asp Tyr Thr Leu Val Asp Ala Val Gly Tyr lie Lys Pro lie 895 900 905 aag aat tta aeg tta cag ttt ggc gtt tat aat ttg aca gac cgt aaa

Lys Asn Leu Thr Leu Gin Phe Gly Val Tyr Asn Leu Thr Asp Arg Lys 910 915 920 tac ttg aca tgg gaa tet get cgt teg att aaa cca ttt ggt aca agt

Tyr Leu Thr Trp Glu Ser Ala Arg Ser lie Lys Pro Phe Gly Thr Ser 925 930 935 aat tta att aat caa aaa aca ggc gca gga att aat cgt ttt tac tea

Asn Leu lie Asn Gin Lys Thr Gly Ala Gly lie Asn Arg Phe Tyr Ser 940 945 950 cca ggt cgt aac ttt aaa etc agt gcc gaa ate acc ttc taatcctaag

Pro Gly Arg Asn Phe Lys Leu Ser Ala Glu lie Thr Phe 955 960 965 cctgcgtatg caggctttct ttttagggaa agtgcggtgg atttgacaaa gatttattgc ttttctgtaa atcaatgcta aaattcacac tcctttgtcg tagctggatt agagategge tagcgatgta tttttaactt aacttttagg agttatcaaa tgtetetaag tacagaaaaa aaagcagcaa ttgttgctga atttggtcgt gatqcaaaaq ataccggttc ttcaaaaatg 136852-序列表.doc 76- 7047 7095 7143 719X 7239 7287 7335 7383 7431 7479 7527 7575 7623 7672 7732 7792 7852 7912 1334884

caaatcgcat tattaactgc acaaatcaac cacttacaat ctcactttgc aacgcacaaa 7972 aaagaccacc acggtcgtcg tggtttattg cgtatggttt ctcgtcgtcg taaactttta 8032 gattacttaa aacgtactaa tcttgagctt tacacttcaa ctatcgctcg tttaggttta 8092 cgtcgctaat ttgtattagg atttattcca aacaaaaaac ccttgataat tttatcaagg 8152 gttttctttt ttctgcatac taggcatgtt taaattatcg caaaacacac cgcacatttc Θ212 gtggaaaagt gcggtcattt ttttaattta ttttacttct ttaaacatga tctcacttgg 8272 gattactgaa ccttgccagt aaagctcagt agcgactttt tcagctaatt gcataaacga 8332 ttgggcaatg tcgctttcag gtgcggcgac aacggttggg atacctttgt ctaaatcttc 8392 acgtaagcga atatgtaatg cctgttgtcc taaaactttg acattatatt tttgtgcaat 8452 gcgctcagca ccgcctgttc cgaaaatcgt ttcttgatga ccacaattgc tacaaatatg 8512 catcgacata ttttcgataa tgcctaaaac gggtacagaa acacgctcaa acatcgccac 8572 acctttaatc gcatccagta aagcaatatc ttgtggtgtt gtcaccacga ccgcccccgt 8632 cactggaatt tgttgagaaa gggtcagctg gatatcccct gtacccggtg gcatatcaat 8692 gactaaataa tctaaatcag gccataaggt ttcttgcaaa agctgactta aggcactgct 8752 tgccattgga ccgcgccaaa tcgtagcatt gtccggttcc attaagaaac caatggaatt 8812 ggcaaaaata tgatgtgctt gaattggggt aatgtgctgg ttatctggcg aagttgggcg 8872 ttgatcagca acccctaaca tgtgtggaat agatggacca taaatatcgg catctaaaat 8932 tccaacacga gcaccttgtc tttgtaaggc aagagcaaga ttgacggaaa tagtagattt 8992 tcctacacca cctttacccg atgtcacggc aataatattt tttacccctt ttacggctgg 9052 gtggctatta gcgcgtttta atgtcgcgat ttgataattt aattgccatt tgatgtcttt 9112 gcattctgct aatgtgagaa gttctgtgga gagagcgctc ttgagttgtt cgaatgcagt 9172 attccaagca aacggcatgc tgatttcaat acgtaacgtc tcaccgcctt tttcaatttt 9232 tttgatagca tttaggctga cgagatcttt ttgtaagcta ggatgttgaa attgttgaaa 9292 tgtgttttgg atagcttgtt tttgactgtc cgttaaactg tcagaaaata aaattcccat 9352 Cgattttatc ccgttatttt tggtggctaa ttaaagccct agttaatcac tcaactatat 9412 ttaaccacga agctgtagaa ctgttaagca gaaatgtgga aaagcgcggt taaagtagaa 9472 aaaatactgc gaataaggta acataagcgc caattttttg atgaaaaata ggaatgataa 9532 catggcaaat tcggcacgcg atattttggt cacttgcgcc ttaccttatg caaatggtgc 9592 aattcattta gggcatttat tagaacatat tcaagcagat atttgggtgc gttccaacgt 9652 atgcgtgggc ataaagtgca ttttatttgt gcagatgatg cccatggcac accaatcatg 9712 ttaaatgcga taaattaggt attacaccaa agcattaatc gctcctcgaa agcagaacat 9772 gtggcggatt tgaaggctta atattagcta tgataatatc at 9814 -77- 136852-序歹!I 表.doc

1 SI <210> 32 <211> 967 <212>蛋白質 <213>敗血性巴斯德样菌 <400> 32

Met Arg Thr Thr Thr lie Lys Phe Ser Ala He Thr Leu Ala Leu Leu 1 5 10 15 Ser Tyr Cys Gly Thr He Leu Ala Asp Ser His Gin Glu Ala Thr Glu 20 25 30 Leu Asp Thr lie Thr Val Ser Ser Gin Gin Asp Glu Met Asn lie Lys 35 40 45 Glu Lys Lys lie Gly Glu Thr Val Lys Thr Ala Ser Gin Leu Lys Arg 50 55 60 Gin Gin Val Gin Asp Ser Arg Asp Leu Val Arg Tyr Glu Thr Gly Val 65 70 75 80 Thr Val Val Glu Ala Gly Arg Phe Gly Ser Ser Gly Tyr Ala lie Arg 85 90 95 Gly Val Asp Glu Asn Arg Val Ala lie Thr Val Asp Gly Leu His Gin 100 105 110 Ala Glu Thr Leu Ser Ser Gin Gly Phe Lys Glu Leu Phe Glu Gly Tyr 115 120 125 Gly Asn Phe Asn Asn Thr Arg Asn Ser Val Glu lie Glu Thr Leu I»ys 130 135 14 0 Val Ala Lys lie Ala Lys Gly Ala Asp Ser Val Lys Val Gly Ser Gly 145 150 155 160 Ser Leu Gly Gly Ala Val Leu Phe Glu Thr Lys Asp Ala Arg Asp Phe 165 170 175 Leu Thr Glu Lys Asp Trp His lie Gly Tyr Lys Ala Gly Tyr Ser Thr 180 185 190 Ala Asp Asn Gin Gly Leu Asn Ala Val Thr Leu Ala Gly Arg Tyr Gin 195 200 205 Met Phe Asp Ala Leu lie Met His Ser Lys Arg His Gly His Gill Leu 210 215 220 Glu Asn Tyr Asp Tyr Lys Asn Gly Arg Asp He Gin Gly Lys Glu Arg 225 230 235 240 Glu Lys Ala Asp Pro Tyr Thr lie Thr Lys Glu Ser Thr Leu Val Lys 245 250 255 Phe Ser Phe Ser Pro Thr Glu Asn His Arg Phe Thr Val Ala Ser Asp 260 265 270 Thr Tyr Leu Gin His Ser Arg Gly His Asp Leu Ser Tyr Asn Leu Val 275 280 285 Aia Thr Thr His lie Gin Leu Asp Glu Lys Glu Ser Arg His Ala Asn 290 295 300 136852-序列表.doc 78- 1334884

Asp Leu Thr Lys Arg Lys Asn Val Ser Phe Thr Tyr Glu Asn Tyr Thr 305 310 3又S ^20

Val Thr Pro Phe Trp Asp Thr Leu Lys Leu Ser Tyr Ser Gin Gin Arg 325 330 335 lie Thr Thr Arg Ala Arg Thr Glu Asp Tyr Cys Asp Gly Asn Glu Leu 340 345 350

Cys Asp Ser Tyr Lys Asn Pro Leu Gly Leu Gin Phe Lys Asp Gly Gin '355 360 365 lie Leu Asp Pro Ala Gly Asn Lys lie Lys Leu Gin Gly Ser Gly Leu 370 375 380

Ser Thr Gin lie Val Asp Glu Asn Gly Lys Pro Phe Pro Thr Thr Thr 385 390 395 400

Gly Thr Asn Asn Ala Ala Phe Ser Asn Asn Leu Arg Leu Arg Pro Thr 405 410 415

Gly Phe Trp Leu Asp Cys Ser Val Phe Asp Cys Asn Lys Pro Phe Thr 420 425 430

Val Tyr Asn lie Ser Asn Gly Thr Tyr Gin Ala Arg Glu Val Leu Leu 435 440 445

Ser Glu Glu lie Thr Val Asp Gly Lys Leu Tyr Lys Thr Ala Lys Glu 450 455 460

Glu Gly Gly Leu Pro Asn Tyr Leu lie Leu Pro Asn Ser Lys Gly Tyr 465 470 475 480

Leu Pro Tyr Asp Tyr Lys Glu Arg Asp Leu Asn Thr Asn Thr Lys Gin 4Θ5 490 495 lie Asn Leu Asp Leu Thr Lys Thr Phe Leu Thr Phe Asn lie Glu Asn 500 505 510

Asn Leu Ser Tyr Gly Gly Val Tyr Ser Arg lie Glu Lys Glu Met lie 515 520 525

Asn Lys Ala Gly Tyr Glu Gly Arg Asn Pro Thr Trp Trp Ala Asp Arg 530 535 540 lie Leu Gly Gin Ser Ser Tyr Cys Gly Tyr Asn Ala Leu Lys Cys. Pro 545 550 555 560

Lys His Glu Pro Leu Thr Ser Phe Leu lie Pro Val Glu Ala Thr Thr 565 S70 575

Gin Ser Leu Tyr Phe Ala Asn lie Leu Lys Val His Asn Met lie Ser 580 585 590 lie Asp Leu Gly Tyr Arg Tyr Asp His lie Lys Tyr Asn Pro Glu Tyr 595 600 605

Thr Pro Gly Val Thr Pro Lys lie Pro Asp Asp Met Val Lys Gly Leu 610 615 620 Phe He Pro Met Pro Lys Glu Pro Gin Leu Lys Asp Phe Asp Tyr Asn 625 630 635 640 •79· 136852·序列表.doc 1334884

Tyr Ala hys Phe Uly Uiu Aia Tyr bys l#ys Trp Ijys Glu Tyr lieu Pro 645 650 655

Lys Asn Ala GIu Giu Asn lie Ala Tyr lie Ala Gin Asp Lys Thr Phe 660 665 670

Lys i»ys His Ser Tyr Ser Leu Gly Ala Thr Phe Asp Pro Leu Asn Phe 675 680 685

Leu Arg val Gin Val Lys Tyr Ser Lys Gly Phe Arg Ala Pro Thr Ser 690 695 700

Asp Giu Leu Tyr Phe Thr Phe Lys His Pro Asp Phe Thr lie Leu Pro 705 710 715 720

Asn Pro Val Leu Lys Pro Giu Giu Ala Lys Asn Gin Giu lie Ala Leu 725 730 735

Thr Val His Asp Asn Trp Gly Phe Val Ser Thr Ser Val Phe Gin Thr 740 745 750

Lys Tyr Arg His Phe lie Asp Leu Ala Tyr Leu Gly Ser Arg Asn Leu 7SS 760 765

Ser Asn Ser Val Gly Gly Gin Ala Gin Ala Arg Asp Phe Gin Val Tyr 770 775 780

Gin Asn Val Asn Val Asp Asn Ala 785 790 Ala Arg Leu Asn Leu Gly Tyr Phe 805 Thr Ser Tyr Lys Phe Thr Tyr Gin 820 Pro Met Asn Ala lie Gin Pro Lys 835 840 Asp His Lys Giu Asn Lys Phe Gly 850 855

Lys Val Lys Gly Leu Giu lie Asn 795 800 Trp His Val Leu Asp Gly Phe Asn 810 815 Arg Gly Arg Leu Asp Gly Asp Arg 825 830 Ala Ser Val Phe Gly Leu Gly Tyr 845 Ala Asp Leu Tyr lie Thr Arg Val 860

Ser Giu Lys Lys Ala Lys Asp Thr Tyr Asn Met Phe Tyr Lys Giu Gin 865 870 875 880

Gly Tyr Lys Asp Ser Ala Val Arg Trp Arg Ser Asp Asp Tyr Thr Leu 885 890 895

Val Asp Ala Val Gly Tyr lie Lys Pro lie Lys Asn Leu Thr Leu Gin 900 905 910

Phe Gly Val Tyr Asn Leu Thr Asp Arg Lys Tyr Leu Thr Trp Giu Ser 915 920 925

Ala Arg Ser lie Lys Pro Phe Gly Thr Ser Asn Leu lie Asn Gin Lys 930 935 940

Thr Gly Ala Gly He Asn Arg Phe Tyr Ser Pro Gly Arg Asn Phe Lys 945 950 955 960

Leu Ser Ala Giu lie Thr Phe 965 80- 136852-序列表.doc i s] 1334884

<210> 33 <211> 2990 <212> DNA <213>敗血性巴斯德样菌 <220>

<221> CDS <222> (1106)..(1564) <^>20> <223> kdtB <400> 33

gtcaacccct ttggctttga gttgtgctaa taaagcatcg tcaaaatgca agccggcagt 60 tggtgccgca accgcgccag ggactttgtt ataaacggtt tgataacgtt ctttatccgc 120 ttcttcgtca gggcgatcaa tataaggggg caatggcata tgcccaattt gctgtaacac 180 gtctaaaagt gcggtctgtt tttgcgcgat ttctaattca aataaggtat catggcgcgc 240 aaccatgatc attttgacac catgatgttc acctaactta tcttcgccta accacagttc 300 tgccccttct ttcggtgctt ttgaggagcg cacatgggct aaaaagcgtg tgtcggataa 360 aatccgctcg accaacactt ccaccttacc gccactggct ttacgtccaa acatccttgc 420 aggaatcacg cgcgtgttat taaaaattaa taagtcgcct tcatgaattt gatcaaggat 4β0 atcagcaaaa gtgcggtggg taatctcacc attttcgccg ttaagttgta ataagcgact 540 agcggtgcga tccggttttg ggtaacgagc aatcagctca tcgggtaaat caaaataaaa 600 gtcagaaaca cgcataaata gggttataaa aagttatcta aaaaatcgtg ggcgtaagtc 660 tagtgtgaat tccgctcttg cacaaggaaa aatccagatt ttgttgttta gtatcgaatt 720 gagatgattt tggacaaaaa aaaagccctt tcaagaaaga cgaaagggcg aaaatatatt 780 tggagtcata ctttttaggg tatgtgtcgg attatacaca caaaaataac aaatgcaaca 840 tttttttaac aatcatatgt aagcgtattg tgtgagaacg agcgtaaaaa tgaacgcatt 900 ctaaaggatg atttatttag cctattaaaa aaacacatga gatgagagtt tgcgagagcg 960 gtaataaaag tgcggtgggt tttagaaaag ttttgaatag gatcacaaat taaacaaagt 1020 ttgtgaaata ccaagtagta gtttttaagt ;atatgatgaa teatatgeta . aagtttaaac 1080 ccgttaaata accaagaggt ggaag atg aca gaa gaa aat aaa gga aag aga 1132 Met 1 Thr Glu Glu Asn 5 Lys Gly Lys Arg tat ttt tta tgg t tc ata ttg ttt ate ett tea ate tat tta ttt att 1180 Tyr Phe Leu Trp Phe lie Leu Phe lie Leu Ser lie Tyr Leu Phe lie 10 15 20 25 acc ata caa gaa aga cga ggt tat tgt ttt gac aaa cgt gca tat att 1228 Thr He Gin Glu Arg Arg Gly Tyr Cys Phe Asp Lys Arg Ala Tyr lie 30 35 40 1334884 cat. gag ctt tat act gag caa gag tta att gat egg ggg att gaa tat 1276

His Glu Leu Tyr Thr Glu Gin Glu Leu lie Asp Arg Gly lie Glu Tyr 45 50 55 gtg gta tcc acc atg ccg tea ggt gtt att aaa cca gat ggc aca ata 1324

Val Val Ser Thr Met Pro Ser Gly Val lie Lys Pro Asp Gly Thr lie 60 65 70 aaa gaa gta aag cgt tac aeg agt gtc gag gag ttt aaa cag atg aac 1372

Lys Glu Val Lys Arg Tyr Thr Ser Val Glu Glu Phe Lys Gin Met Asn 75 80 85 cca get tgt tgt aca tta acc acc ttt att gat gaa gga ggc gat ggc 1420

Pro Ala Cys Cys Thr Leu Thr Thr Phe He Asp Glu Gly Gly Asp Gly 90 95 100 105 tat cca gat gat gat gga tat ggt tat gtc aga att gaa tat tta aga 1468

Tyr Pro Asp Asp Asp Gly Tyr Gly Tyr Val Arg lie Glu Tyr Leu Arg 110 115 120

cat tat gtt gag aat eta aaa cct tat cat aga gtg att tat ctt gaa 1516

His Tyr val Glu Asn Leu Lys Pro Tyr His Arg Val lie Tyr Leu Glu 125 130 135 tat aeg ccc tgt gga gag tta agg gaa gag geg get ttt tea aaa aat 1564

Tyr Thr Pro Cys Gly Glu Leu Arg Glu Glu Ala Ala Phe Ser Lys Asn 140 145 150 taagagtgag gtgaagaaat ggcattacca acagcaacaa taatgaggaa tttatcttta 1624 tctaaaaatc aattcactct gaaagggatg gaatgegtag attccctatt tcaagcatgc 1684 agtaatatgg atcatgggta ctgaggtgga agatggeaga agaaaataaa ggaaagagat 1744 attttttatg gttcatattg tttatccttt caatctattt atttattacc atacaagaaa 1804 gacgaggtta ttgttttgac aaatgggaat atatccataa cctttatacc gagcaagagt 1864 tgategatag aggggttgaa tatgtggtat ccaccatgcc gtcaggtgtt tttgaaccag 1924 atggcacaac aaccgaaata aaacgttatg ctagtgttga ggagtttaaa cagatgaacc 1984

ctgattgttg taaattaaca agatttatta atgaaggaat agatggetat ccagatgatg 2044 atggatatgg ttatataaga attgaatatt taagacatta tgttgggaat tttaaacctg 2104 atcatagagt gctttatctc gaatataege cttgtggaga attaagggaa gaggtttctt 2164 tttaaaaaat aaataatagt gaggtgaaga aatggcatta ccaacagcaa cagaaatcac 2224 aaatgeatat ttatataaaa ataaattaac tcctaaagcg gaggaaagag tagattcaat 2284 acaaattctt gaaaaaggag atgaacattt cgaagtaaat tttaattgat caaagtactc 2344 tattgattga aggaaaaaca gtggaattaa tggcaggtat ggcagtttct geggaaatta 2404 aaacaggtaa acgcagtgta ttagattact tatttagccc attaaaaacc acaaaataat 2464 attaaggaga ataatatgtc gtataataaa tatactgttg ctttgattac gttctcaaca 2524 gggatctgta ttccggcaat atgetaeget etaaattege tgggatacag atcctgtctg 2584 agactatgta gaaaagacta aactttgtgt ggttaactgg gcttcggtaa aattctggaa 2644 •82· 136852·序列表.doc 1334884 acaaatgggc ttaacccgcg tgatcttatc ccgtgagctt tcgcttgatg aaattgccga 2704 aattcgtcag caagtgccag aaatggaaat tgaagtgttc gtgcatgggg cattatgcat 2764 ggcgtattct ggacgttgtt tattatcagg ctatattaat aaacgtgatc caaatcaagg 2824 cacctgtacc aatgcgtgcc gttgggaata cagtgtaacc gaagccaaag aagatgagat 2884 cggcaacatt gtgaatgtgg gtgaagaaat tccagtgaaa aatgtagcac cgacacttgg 2944 cgaaggcgac accaccagta aagtattttt attagcagaa agtcga 2990 <210> 34 <211> 153 <212>蛋白質 <213>敗血性巴斯德桿菌 <400> 34

Met Thr Glu Glu Asn Lys Gly Lys Arg Tyr Phe Leu Trp Phe lie Leu IS 10 15

Phe He Leu Ser lie Tyr Leu Phe lie Thr lie Gin Glu Arg Arg Gly 20 25 30

Tyr Cys Phe Asp Lys Arg Ala Tyr He His Glu Leu Tyr Thr Glu Gin 35 40 45

Glu Leu lie Asp Arg Gly He Glu Tyr Val Val Ser Thr Met Pro Ser 50 55 60

Gly Val lie Lys Pro Asp Gly Thr lie Lys Glu Val Lys Arg Tyr Thr 65 70 75 ' 80

Ser Val Glu Glu Phe Lys Gin Met Asn Pro Ala Cys Cys Thr Leu Thr 85 90 95

Thr Phe lie Asp Glu Gly Gly Asp Gly Tyr Pro Asp Asp Asp Gly Tyr 100 105 110

Gly Tyr Val Arg lie Glu Tyr Leu Arg His Tyr Val Glu Asn Leu Lys 115 120 125

Pro Tyr His Arg Val lie Tyr Leu Glu Tyr Thr Pro Cys Gly Glu Leu 130 135 140

Arg Glu Glu Ala Ala Phe Ser Lys Asn 145 150

<210> 35 <211> 1683 <212> DNA <213>牧血性巴斯德桿苗 <220>

<221> CDS <222> (325) . . (1230) <220>

<223> IgtC -83 - 136852·序列表.doc ISl <220> <221> misc—待徵 <222> 219" <223> Xaa=任何或未知的胺基酸 <220> <221> misc_#& <222> 226 ~ <223> Xaa=任何或未知的胺基酸 <220> <221> misc_特徵 <222> 269 ~ <223> Xaa=任何或未知的胺基酸 <220> <221> misc_ 特徵 <222> 270 ~ <223> Xaa=任何或未知的胺基酸 <220> <221> misc 特徵 <222> 274 ~ <223> Xaa=任何或未知的胺基酸 <400> 35 atatcaaagt ctcatggcaa gaaaattaga aaagagcgat caattattat ttgcaagatt 60 tgggtattat tcataggcta ggtgaaagat atatttttcc atgatattaa aacgattcag 120 gcagaactgg ctagcttatc acttttagat aattgtatta ttaaaagaag ctgtatgatt 180 gttattctat cattagtgga taataaatat tctttatttt ttgagagata aaaacaattc 240 atatttcaat agaaaacaga aaataaagat tatcaaaaga attatccgtc cttataaata 300 tgagtctgta ttgtgagatg atat atg aat att tta ttt gtt tct gat gat 351

Met Asn lie Leu Phe Val Ser Asp Asp 1 5 gtt tat get aaa cat ctg gtg gtt geg att aaa age act ata aat cat 399

Val Tyr Ala Lys His Leu Val Val Ala lie Lys Ser lie lie Asn His 10 15 20 25 aat gaa aaa ggt att tea ttt tat att ttt gat ttg ggt ata aag gat 447

Asn Glu Lys Gly lie Ser Phe Tyr lie Phe Asp Leu Gly lie Lys Asp 30 35 40 . gaa aat aag aga aat att aat gat att gtt tct tct tat gga agt gaa 495

Glu Asn Lys Arg Asn He Asn Asp He Val Ser Ser Tyr Gly Ser Glu 45 50 55 gtc aac ttt att get gtg aat gag aaa gaa ttt gag agt ttt cct gtt 543

Val Asn Phe lie Ala Val Asn Glu Lys Glu Phe Glu Ser Phe Pro Val 60 65 70 caa att agt tat att tct tta gca aca tat gca agg eta aaa geg gca 591

Gin lie Ser Tyr lie Ser Leu Ala Thr Tyr Ala Arg Leu Lys Ala Ala 75 80 85 gag tat ttg ccg gat aat tta aat aaa att att tat tta gat gtt gat 639

Glu Tyr Leu Pro Asp Asn Leu Asn Lys lie lie Tyr Leu Asp Val Asp 90 95 100 105 136852·序列表.doc 84 _ 1334884 gtt ttg gtt ttt aac tea tta gaa atg tta tgg aat gtt gat gtt aat 687

Val Leu Val Phe Asn Ser Leu Glu Met I.eu Trn Asn Val Asp Val Asn 110 1X5 ' 120 aat ttt ett acc gca gcc tgt tat gat tet ttc ate gaa aat gaa aag 735

Asn Phe Leu Thr Ala Ala Cys Tyr Asp Ser Phe lie Asn Glu Uys 125 · 130 135 tet gag cat aaa aaa teg att tea atg tea gat aag gaa tat tat ttt 783

Ser Glu His Lys Lys Ser lie Ser Met Ser Asp Lys Glu Tyr Tyr Phe

140 145 ISO aat gca gga gta atg eta ttt aat tta gat gaa tgg egg aag atg gat 831

Asn Ala Gly Val Met Leu Phe Asn Leu Asp Glu Trp Arg Lys Met Asp 155 160 165 gta ttc tea aga get tta gac ctg tta get atg tat cct aat caa atg 879

Val Phe Ser Arg Ala Leu Asp Leu Leu Ala Met Tyr Pro Asn Gin Met 170 175 180 1Θ5

att tat cag gat caa gat ata ttg aat ate ett ttt agg aat aaa gtc 927

He Tyr Gin Asp Gin Asp lie Leu Asn lie Leu Phe Arg Asn Lys Val 190 195 200 tgt tat tta gat tgc aga ttt aat ttc at^ cca aat caa ett gaa aga 975

Cys Tyr Leu Asp Cys Arg Phe Asn Phe Met Pro Asn Gin Leu Glu Arg 205 210 215 ata aan caa tac cat aaa gga aaa ntg age aac tta cat tet tta gaa 1023 lie Xaa Gin Tyr His Lys Gly Lys Xaa Ser Asn Leu His Ser Leu Glu 220 225 230 aaa aca aeg atg cct gtc gtt att tea cat tat tgt ggt cca gaa aaa 1071

Lys Thr Thr Met Pro Val Val lie Ser His Tyr Cys Gly Pro Glu Lys 235 240 245 geg tgg cat geg gat tgt aaa cat ttt aat gta tat ttc tat cag aaa . 1119

Ala Trp His Ala Asp Cys Lys His Phe Asn Val Tyr Phe Tyx Gin Lys 250 255 260 265 ata tta gca naa atn teg aga ggc neg gat aaa gaa ege gta tta tet 1167 lie Leu Ala Xaa Xaa Ser Arg Gly Xaa Asp Lys Glu Arg Val Leu Ser 270 275 280

ata aaa act tat etc aag gcc ttg att aga agg att aga tat aaa ttc 1215 lie Lys Thr Tyr Leu Lys Ala Leu lie Arg Arg lie Arg Tyr Lys Phe 285 290 295 aaa tat caa gtc tat taactattga atttttgcaa atgagataag agtatagtgc 1270 Lys Tyr Gin Val Tyr 300 tgatttcttc aaagcgaaaa ggaggaaata gcttgttcta atttattaca ataatggttg 1330 tattcatctt gattttgaag gaaagagagt gttttttgta taaaagcatt ttcgtcacct 1390 aaatttacta atcctccaaa ttctcctcct cgnagaattt ctttcggacc ggtagggcag 1450 tccatggata ttacaggtgt accgcaagcc atgettteta ggataactgt cggtaacccc 1510 tctttcaaag aggtgtgtaa aaatagccta gcatttttta ttaatggata eggattatet 1570 ttatttccta aaaqaaaaca atettettat aqattqaqtg attctatttg tttctctaat 1630 -85 · 136852-序列表.doc i 1334884 ttttctcgac actcaccatc ccaaacaata tatancnttt cttggatacc tcc 1683 <210> 36 <211> 302 <212>蛋白質 <213>敗血性巴斯德样菌 <220> <221> mjsc_ 特微 <222> 219 ~ <223〉父助=任何或未知的胺基酸 <220> <221> misc 特微 <222> 226 " <223> Xaa=任何或未知的胺基酸 <220>

<221> misc 特微 <222> 269* <223> Xaa=任何或未知的胺基酸 <220> <221> misc 特微 <222> 270 <223> Xaa=任何或未知的胺基酸 <220> <22l> misc 特微 <222> 274*" c223> Xaa=任何或未知的胺基酸 <400> 36 Met Asn lie 1 Val Ala lie

Tyr lie Phe 35 Asp lie Val 50 Glu Lys Glu 65 Ala Thr Tyr Asn Lys lie

Glu Met Leu 115 Tyr Asp Ser 130

Leu Phe 5 Lys Ser 20 Asp Leu Ser Ser Phe Glu Ala Arg 85 lie Tyr 100 Trp Asn Phe lie

Val Ser Asp Asp He lie Gly lie

Asn His 25

Tyr Gly 55 Ser Phe 70 ’ Leu Lys Leu Asp Val Asp

Lys Asp 40 Ser Glu Pro Val Ala Ala

Val Tyr Ala 10 Asn Glu Lys Glu Asn Lys

Glu Asn 135

Val Asp 105 Val Asn 120 Glu Lys

Val Asn Phe 60 Gin lie Ser 75 Glu Tyr Leu 90 Val Leu Val

Asn Phe Leu

Lys His Leu Val 15 Gly He Ser Phe 30 Arg Asn lie Asn 45 lie Ala Val Asn

Ser Glu His 140

Tyr lie Ser Leu 80 Pro Asp Asn Leu 95 Phe Asn Ser Leu 110 Thr Ala Ala Cys 125 Lys Lys Ser lie 86, 136852-序列表.doc isi 1334884

Ser Met Ser Asp Lys Glu Tyr Tyr Phe Asn Ala Gly Val Met Leu Phe 145 150 155 160

Asn Leu Asp Glu Trp Arg Lys Met Asp Val Phe Ser Arg Ala Leu Asp 165 170 175

Leu Leu Ala Met Tyr Pro Asn Gin Met lie Tyr Gin Asp Gin Asp lie 180 185 190

Leu Asn lie Leu Phe Arg Asn Lys Val Cys Tyr Leu Asp Cys Arg Phe 195 200 205

Asn Phe Met Pro Asn Gin Leu Glu Arg lie Xaa Gin Tyr His Lys Gly 210 215 220

Lys Xaa Ser Asn Leu His Ser Leu Glu Lys Thr Thr Met Pro Val Val 225 230 235 240 lie Ser His Tyr Cys Gly Pro Glu Lys Ala Trp His Ala Asp Cys Lys 245 250 255

His Phe Asn Val Tyr Phe Tyr Gin Lys lie Leu Ala Xaa Xaa Ser Arg 260 265 270

Gly Xaa Asp Lys Glu Arg Val Leu Ser lie Lys Thr Tyr Leu Lys Ala 275 280 285

Leu lie Arg Arg lie Arg Tyx Lys Phe Lys Tyr Gin Val Tyr 290 295 300 <210> 37 <211> 2029 <212> DNA <213> 敗血性巴斯德样菌 <220> <221> CDS <222> (2) . . (499) <220> <223> mglB <220> <221> misu特徵 <222> 33 <223> Xaa=任何或未知的胺基酸 <220> <221> misc特徵 <222> 99 <223> Xaa=任何或未知的胺基酸 <220> <22X> misc特徵 <222> 101 ' <223> Xaa=任何或未知的胺基酸 <220> <221> misc特徵 <222> 928 ' <223> n=A或T或ϋ或c

87- 136852·序列表.doc 1334884 <220> <221> misc 特徵 <222> 1〇〇7~

<223> n=A<T或C]或C <220>

<221> inisc_特微 <222> |74(Γ <223> ιι=Α或丁或CJ或C <220> <221> misc_特徵 <222> 1808~

<223> n=A或T或C5或C <220> <221> misc 特徵 <222> 1816—

<223> rv=A或T或G或C

<220> <221> misc_特徵 <222> 182(Γ <223> η=Α或Τ或G或C <400> 37 c tta aat aaa gcc ggt aaa att caa tac gtt tta tta aaa ggt aac caa 49 Leu Asn Lys Ala Gly Lys lie Gin Tyr Val Leu Leu Lys Gly Asn Gin 15 10 15 gga cac cca gat gca gaa get cgt aca aaa ttc gtc att aaa gaa tta 97

Gly His Pro Asp Ala Glu Ala Arg Thr Lys Phe Val lie Lys Glu Leu 20 25 30 nat aat aaa ggc att caa gat gag caa tta ttc ate gac aeg ggg atg 145

Xaa Asn Lys Gly lie Gin Asp Glu Gin Leu Phe lie Asp Thr Gly Met 35 40 45 tgg gat gcc get tta geg aaa gat aaa atg gat gca tgg tta tet age 193

Trp Asp Ala Ala Leu Ala Lys Asp Lys Met Asp Ala Trp Leu Ser Ser 50 55 60

tet aaa gca aat caa att gaa gtg ate ate get aac aac gat ggt atg 241

Ser Lys Ala Asn Gin lie Glu Val lie lie Ala Asn Asn Asp Gly Met 65 70 75 80 geg atg ggg gca ttg gaa gcc aeg aaa gca cat ggt aaa aaa tta cca 289

Ala Met Gly Ala Leu Glu Ala Thr Lys Ala His Gly Lys Lys Leu Pro. 85 90 95 ate ttc ngt gta nat geg tta cca gaa gtc etc caa tta ate aaa aaa 337

He Phe Xaa Val Xaa Ala Leu Pro Glu Val Leu Gin Leu lie Lys Lys

100 105 HO ggt gaa att gca ggt aeg gtg tta aat gac ggt gtg aac caa ggt aaa 385

Gly Glu lie Ala Gly Thr Val Leu Asn Asp Gly Val Asn Gin Gly Lys 115 120 125 gee gtt gtt caa tta agt aac aat ett gca aaa gga aaa cct gcc act 433

Ala Val Val Gin Leu Ser Asn Asn Leu Ala Lys Gly Lys Pro Ala Thr 130 135 140 -88 - 136852·序歹(I表.doc

Li'i 1334884 gaa ggc aca aaa tgg cag tta aaa cga teg tgt cct aeg tat ccc tta 481

Glu Glv Thr Lys Trp Gin Leu l*ys Arg Ser Cvs Pro Thr Tyr Pro Ιβυ 145 ' 150 ' 155 160 tgt tgg tgt gga tgc gga taacttaai这c gagttcctaa aataataaac 529

Cys Trp Cys Gly Cys Gly 165

tataacaaaa caagamgttg taattetegg ggaggtatac cctccccctt tttatgtgag 589 gtcggatatg acaactcaaa ttccaaatca agacagtgaa atactgctca caatgaccaa 649 cgtctgtaaa tcctttcccg gtgttaaagc gttagacaat gcaaacctaa ctgtgcgctc 709 gcattctgtc catgccttaa tgggcgaaaa tggggcgggc aaatcgacct tattaaaatg 769 cttatttggt atttacagta aagatgaagg tgacatcctt ttettaggea agccagtcaa 829 ctttaaaacg tegaaagaag ccttagagaa cgggattttc atggtgcacc aagaacttaa 8Θ9 cttggttaaa caatgtactg taatggataa tcctttggnt aggaegttat ccattaaaag 949 caggctttgt cgatcacggc aaaatgtatc gtgataccaa agcagatttt tgaagaanta 1009 gatategata tcgatccaaa agaaaaagtg gccaaattgt cagtgtcaca aatgcaaatg 1069 ategagateg caaaggcctt ttcatacaat gccaaaatcg taatcatgga cgaaccgact 1129 tettegettt cagaaaaaga agttgaacac ctatttaaaa ttatcgcgaa gctaaaacaa 1189 cgtggctgtg gcattattta tatttcgcac aaaatggacg aaatcttcaa aatttgtgac 1249 gaaattacga ttttacgcga tggtaaatgg atcaatacgg tcgctgttaa aggcaccaca 1309

cccaccaaaa. 1369 taatcagcca 1429 tgcgggactg 1489 aacgtaaatc 1549 aagccattaa 1609 cgaatctcag 1669 agttaggttt 1729 atgaatgtga 1789 caaaaagtgg 1849 ccaacacgtg 1909 gccaaaaaag 1969 atggatcaga accaataccc tctattcaag gttggggcaa tggtgtgatt caatggtttt tattgagttt attgagtaac aaacgccatc tcattggtcg gtatcgacat ataaaggtat ttgtatccat caaaagaaac atgttagttt aacggtaccg aaactacacg gccttggtca aactcattaa ncaaaaatga acaaaaccna ctggttatta tggtgcgaaa catcatgatt gatggttggg catcttaacg tgaattaege atattgtgga ataaggaaat eggaagaaeg tttctaacat aaagcgacac tattggntca acccaccctg tatgaaattt teatetaaag cgtgaactca gtggaaaatc aaaggegaag aaegatette gaaaaaccgg tcgctctaca gaagaaatcc gcaatggggt ntatctgggg aaatettgat atcagctgat gccagagtta cgcaacgttt tgaccgcact tgeteggeat ggggtgcgtg aatgcgttcg gggatttatg tatatcagca cattgattcc tggtaaccaa gttagacgaa tatggagtta ttaggggtac <210> 38 <2ll> 166 <212=>蛋白質 <213>敗血性巴斯德样菌 • 89 - 136852-序列表.doc ιζι <220>1334884

<221> rnisc 待微 <222> U <223> Xaa=任何或未知的胺基酸 <220> <221> misc一特徵 <222> 99 " <223> Xaa=任何或未知的胺基酸 <220> <221> misc 特徵 <222> 101 " <223> Xaa=任何或未知的胺基酸 <400> 38

Leu Asn Lys Ala Gly Lys lie Gin Tyr Val Leu Leu Lys Gly Asn Gin 1 5 10 15

Gly His Pro Asp Ala Glu Ala Arg Thr Lys Phe Val lie Lys Glu Leu 20 25 30

Xaa Asn Lys Gly He Gin Asp Glu Gin Leu Phe lie Asp Thr Gly Met 35 40 45

Trp Asp Ala Ala Leu Ala Lys Asp Lys Met Asp Ala Trp Leu Ser Ser 50 55 60

Ser Lys Ala Asn Gin lie Glu Val lie lie Ala Asn Aen Asp Gly Met

65 70 75 BO

Ala Met Gly Ala Leu Glu Ala Thr Lys Ala His Gly Lys Lys Leu Pro Θ5 90 95 lie Phe Xaa Val Xaa Ala Leu Pro Glu Val Leu Gin Leu lie Lys Lys 100 105 110

Gly Glu He Ala Gly Thr Val Leu Asn Asp Gly Val Asn Gin Gly Lys 115 120 125

Ala Val Val Gin Leu Ser Asn Asn Leu Ala Lys Gly Lys Pro Ala Thr 130 135 140

Glu Gly Thr Lys Trp Gin Leu Lys Arg Ser Cys Pro Thr Tyr Pro Leu 145 150 155 160

Cys Trp Cys Gly Cys Gly 165

<210> 39 <2ll> 2628 <212> DNA <213>敗血性巴斯德样菌 <220>

<221> CDS <222> (326)..(766) <220> <223> mioC <400> 39 90· B6852-序歹丨J表.doc 1334884 gccaactaga gtaaaataga cacacttaat tacattgtag aggaatcctt ttacgtcttt 60 agaaatutta gatcagttag aagaaaaaat taaacaagcg gttgaaacta tccaactacc 120 tcaattggaa attgatgaat taaaagaaaa aaataaccaa tcccaacaag caaatgacgc 180 attacgcagt gaaaatgaac aactaaagag tgagcaccaa aactggcaag aacgtttacg 240 ctcattatta ggcaaaattg ataacgtata attcacttct tattaaggct tagtttttcc 300 aagccttatt tttuaggaga aatta atg aaa aca aaa att cgt att ate act 352

Met Lys Thr Lys lie Cys lie lie Thr 1 5 ggc agt aeg ett ggt ggt gca gaa tat gtt gca gaa cat att get gaa 400

Gly Ser Thr Leu Gly Gly Ala Glu Tyr Val Ala Glu His lie Ala Glu 10 15 20 25 ata tta gaa caa caa gat tat cct gta cgt tta gaa cat gga cca aat 448 lie Leu Glu Gin Gin Asp Tyr Pro Val Arg Leu Glu His Gly Pro Asn

30 35 40 ttt gaa gaa gtg ate gat gaa aaa tgt tgg ett gtt gtc acc tet acc 496

Phe Glu Glu Val lie Asp Glu Lys Cys Trp Leu Val Val Thr Ser Thr 45 50 55 cat ggt gca ggt gaa tta ccg gat aat att aaa cct ctg ttt gaa aaa 544

His Gly Ala Gly Glu Leu Pro Asp Asn lie Lys Pro Leu Phe Glu Lys 60 65 70 tta gca ttt cac cca aaa cag tta get gac tta ege ttt geg gtg ate 592

Leu Ala Phe His Pro Lys 61n Leu Ala Asp Leu Arg Phe Ala Val lie 75 80 85 ggg tta ggt aat teg gat tat gat acc ttc tgt cac gca gtg gat cat 640

Gly Leu Gly Asn Ser Asp Tyr Asp Thr Phe Cys His Ala Val Asp His 90 95 100 105 gtg gaa caa tta ctg eta age aaa gat get tta caa ctg tgt gaa teg 688

Val Glu Gin Leu Leu Leu Ser Lys Asp Ala Leu Gin Leu Cys Glu Ser 110 115 120

eta aga atg gat atg eta acc att act gat cct gaa cac aeg gee gaa 736

Leu Arg Met Asp Met Leu Thr lie Thr Asp Pro Glu His Thr Ala Glu 125 130 135 caa tgg etc cca caa ttt etc agt caa tta taatatttat tccctataca 786 Gin Trp Leu Pro Gin Phe Leu Ser Gin Leu 140 145 atggcatatg taaatcaaat atgccatttt teatetegat caagcataat atttaaccaa 846 tcaaatcaat attttctctg tggataacta agatcaaaac tgtataaaag ctgtttttat 906 tccctgaata agattgaatg ttttttattc tgtggataac taaagaagtt attcacagtt 966 ttttctggtg ccaaattgag atcttaacaa cttaaaaaat gatetaagtt attcatttaa 1026 aaaaagaaaa ggatcttaat cacagcacta taggatccta ataatcataa taataagatc 1086 tetttatata aaaagatcct atetttatta actcacgatc tttttcacga tcatcgtaca 1146 gtettgatea aaaatgtttc tttcatggat ccataaattt cagtagaata gccaaccagc 1206 •91 · 136852-序列表.doc ISl 1334884

aaaaaggatc aaaagatcca taaaatccga gataaattaa caaggttact atgttttata 1266 ctgaaaatta tgatgttatt gtgatcgatg gtggacacgc aggtactgaa gctgcacttg 1326 caccggcacg catgggactc aagaccctat tattaaccca taatgttgat acactagggc 1386 aaatgtcttg taatcctgcg attggtagga ttggtaaagg ccatttagtc cgagaaattg 1446 atgcgatggg cggtttaatg gcaactgctg cggaccaagc aggaatccaa tttcgtacct 1506 taaacagcag caaaggaccg gcggtacgtg ctacacgtgc gcaagctgac cgcgttttat 1566 atcgccaagc agtacgtatt gcattagaaa atcaagaaaa tttagatatt tttcaacaag 1626 aagtgaccga tattatttta gatcaggatc gtgtctgcgg tgttgttact aaaatgggtt 1686 taaaatttca cgcaaaagca gtgattttaa cagccggtac tttcctttct ggtaagatcc 1746 acattggttt agaaaattat acaggtggac gcgcgggtga tcctgcttca gtgatgttag 1806 ccgatcgttt aagagaactg aatttacgtg tanatcgttt aaaaacgggt acaccgcccc 1866 gtattgatgc acgtactatt gatttctcaa tactggctaa acaacatggc gatgaaaaat 1926 tacctgtctt ttccttcatg ggatctgttg atcaacaccc acgtcaaatt ccatgtttta 1986 ttacccatac aaatgaacaa acgcatgaag tgatccgtaa taacttacat cgcagcccaa 2046 tgtatgctgg gatcattgaa gggatcggtc cacgttattg cccttctatt gaagataaag 2106 taatgcgttt ttctgagcgt aattctcatc aaatctacct tgaacctgaa gggttgacaa 2166 acaaaacaaa gaaattgcgg atttacaaaa acaagtgcaa gcactgcaag cagatttaag 2226 cgaaatggca aagaaaaacc gcaatcaagc gttgattgca ggtggtattg gcggtggcat 2286 tgttgcagtc ggtattgagc tcattcgctt gcaatttggg ggctaactga tggcatttga 2346 tgaaaaaaca cgtgcgctgg ttcgtcgcta ctatgtattt gagtttttat cgcttgagca 2406 atcagcaagt aaagctaaag tctcatttaa caccgcgcga cgctggaaga aagaggcggc 2466 aagcaagggc gatgactggg ataaagtgcg tgatgtacaa gtaatggcgg gcaatgagct 2526 gactgatatc acaaaaggat tgttatcggg ctttattatt caatatcgcg caaccatgga 2586 tgagattcaa aactcggatt taaaagcaca agataaagtc ga 2628 <210> 40 <21X> 147 c212> 白質 <213>敗血性巴斯德桿菌 <400> 40 Met Lvs Thr Lys

Glu Tyr Val Ala 20 Pro Val Arg Leu 35 lie Cys lie He Thr 5 Glu His lie Ala Glu 25 Glu His Gly Pro Asn 40

Gly Ser 10 lie Leu Phe Glu

Thr Leu Glu Gin Glu Val 45

Gly Gly Ala 15 Gin Asp Tyr 30 lie Asp Glu -92· 136852·序歹!1表.doc 1334884

Lys Cys Trp Leu VaJ· Vai Thr Ser Thr His Gly Ala Gly Glu Leu Pro 50 55 60

Asp Asn lie Lys Pro Leu Phe Glu Lys Leu Ala Phe His Pro Lys Gin 65 70 75 80

Leu Ala Asp Leu Arg Phe A丄a Val lie Gly Leu Gly Asn Ser Asp Tyr 85 90 95

Asp Thr Phe Cys His Ala Val Asp His Val Glu Gin Leu Leu Leu Ser 100 105 110

Lys Asp Ala Leu Gin Leu Cys Glu Ser Leu Arg Met Asp Met Leu Thr 115 120 125 lie Thr Asp Pro Glu His Thr Ala Glu Gin Trp Leu Pro Gin Phe Leu 130 135 140

Ser Gin Leu

145

<210> 41 <211> 5191 <212> DNA <213>敗血性巴斯德桿菌 <220>

<221> CDS <222> (3203) . . (4255) <220> <223> mreB <400> 41 ctgcagtacc aaccacccaa atgtcgtgct tctgctgtaa ttcacgcaaa gtgcgcgcga 60 ggttggttac acggattaat ggcacgactt cagcggcacc acaagctact ttacgtgcga 120 tagcggttaa ttgggcagat ttatcttttg gtactatcac agcacacacg cccgctgcat 1β0 ccgcagtacg caaacaagca cctaagttat gtggatcagt cacaccatct aacactaata 240

gcaacggatt ggactgattt tgtaaaagcg tatctaaatc tgcttcattc aattctttcg 300 ctggctgaac acgtgccatg atgccttggt gtacttcgcc ctctgctttt ttatcaaggg 360 tttggcgatt aacaaattgg atagtaatac ccaaccgatg aagttcattg agcaaaggtt 420 gtaaacgttt atcgtcgcgc cctttgagtg cataaacttc aattaaacgc tctggcgcgt 480 tgtttaaaaa ggcacttact gcatgaatgc cataaatatt ttcactcatc tactttctct 540 ttttagctga tttcttactg gcttttttcg tggttgttga tggtttaaca acgcttgttt 600 ttcttaccgc acttttactg gtgcttggtt tacgtttatg gcgttgtttt tcgagacttt 660 cagcgtagcg tgcatttttc ttgagcttgt ctttggctgt tttgccttct cgtaatggtt 720 tacgctcact cgacactaaa gaaaaatcca cttggcgttg ttcaaggcta actgcttcca 780 cacggatttt taccttatcg ccaatgcggt agatcatacc actattttca ccgattaaac 840 •93- 136852-序列表.doc i s] 1334884

gttgtcgagc gagatcaaat tggtagtagt cgttatctaa ggtagaaatg tggactaagc 900 catcaacaaa gaggtcatct aagcggacaa ataagccaaa acctgtcaca gatgaaatca 960 cacctgtaaa ttcttcgcct acatgatctt gcatatattc gcattttagc cagtctgcaa 1020 catcacgagt ggcatcatcg gcccgtcgtt ctgtcatgga gcagtggtcg cctaatacat 1080 ccatgtcatc aagtgtatag tgataccct.c ccgtatcagt ggttctccgt ttcgagcctt 1140 ttagttcggc caacaagtat ttaatcccac gatgcaaagt caaatcagga taacggcgaa 1200 ttggcgaagt aaaatgcgca tattcttcga gtgcaagccc aaagtgccca atattgtcag 1260 gatgataaac ggcttgactt aacgaacgta atacatggtt tgcaataact catgatctgg 1320 acgttcgcga atttgctcca ataatttagc gtagtctgcg gtacttggtt tactgccacc 1380 ttcaaggctt aaaccacatt cactgaggaa ttggcggaag cccgtcactt tttcttcgct 1440 tggacccgca tgaatacgga aaagggctgg ctcttgatgt ttttccataa agtttgccgc 1500 cgcaatattg gcgaggatca tacattcttc aatgatttta tgcgcatcat tacgaataac 1560 aggctcaatc cgttcgattc gccccatttc attaaacaca aacttacttt caatggtttc 1620 aaagtcaatg gcgccacgtt gatgacgggc ttccactaag gcttggtaca ttacatggag 1680 ttcttttaaa tggggaacca gtgcttgata acgagtacaa agttcttcat cgtcctctaa 1740 aatacgagca actttggtat aggttaagcg agcatgagag ttcatgaccg cttcataaaa 1800 ttcatagcct gtgattttac cttttgctga aacattcagc tcacagacca tacataagcg. 1860 atcgacttgt gggtttaatg aacaaagtcc attggagagg atttccggta gcataggaac 1920 aacgcgattt gggaaatata ccgagtttcc acgcgcatgt gcttcggtat caagggcagt 1980 acgtaaacga acatagtagc tgacatcggc aatggcaacc cacagtttcc aaccgcctcc 2040 acgttttttc tggcaaaaaa ccgcatcgtc aaaatcgcga gcatcttcac catcaatggt 2100 gactaacgga agatggcgta aatcaatacg tcctgatttg gcttcttccg gtacttcttc 2160 actcagttta gaaacttgtt tgaggaccgc gtctggaaaa acatggggaa tatcatgatt 2220 acggatagca atttccacct ccatcccttt tgccatattt tcaccgagaa tttcgctaat 2280 cattccaaca ggttggctaa atgttgcggt acgtggtttt aattcaacta caaccacttg 2340 tcccatacga gcgccttggc ggtgttcatt cggaattaaa atgtcgcgat taattcgact 2400 atcgtcaggt actacataac caataccatc ttctaagaaa aaacgaccaa caatctgttt 2460 2520 2580 2640 2700 2760 tttacgctgt gctaggttgg aaaccaaccg tacggtccct aaccaattgt tgtaagacgc gcgaggacat ctgtctttac tttaataaat ccgtcatttt ggacaatccg agtcaccgtg cttcgacttg ccagtttttc ccatcgctct cacttcttgg cattacccgc taaaaaacca cggtaaagcg taagcgacgg cgaccacgac tgcatttggc taaccatcac tagcgtttac cgcatggctt ggtcaaaacc tgttgggtat gatgacctaa gtttagtgaa cttgtcgttc -94- 136852-序列表,doc IS1 1334884 ttcattttga atagcaaatg tggtcagtaa ttcttctttt gagataggtg cattatgttg 2820 acgaattgta tcaagaataa attcgcgact tgggatcggg ttctcatatt ttgcgagttc 2880 ttcttgatag tttggatctt gcaaatgtgg attgtttttg atttttgcca taatgactcc 2940 ctttgagaat ggaaatacgc attcgtttaa tgacctcaag tttgacactc tgatttgttg 3000 aaagcaagta aaaaacctgt tcgtgactga aacaatctgt actcgcggga tttgtcgtgc 3060 atgaaagcgt aaagacaaag caaacttcga caatttacag aaaattctct gttataaagg 3120 tacatttttt gatacattta gcacaatttt tcaatactca aatcagagtg tccattattt 3180 aatttagttc agcggaattc tt atg tta ttt aaa aaa att cga ggc tta ttt 3232

Met Leu Phe Lys Lys lie Arg Gly Leu Phe IS 10 tea aat gat ctg tcc ate gat ett ggc aca geg aat acc tta att tat 3280

Ser Asn Asp Leu Sex lie Asp Leu Gly Thr Ala Asn Thr Leu lie Tyr 15 20 25 gtc aaa gga caa ggg att gtt tta gat gaa cct tet gtt gtg geg att 3328

Val Lys Gly Gin Gly lie Val Leu Asp Glu Pro Ser Val Val Ala lie 30 35 40 ege caa gaa cgt tea ggt gca tta aaa age att get geg gtt ggt cgt 3376

Arg Gin Glu Arg Ser Gly Ala Leu Lys Ser lie Ala Ala Val Gly Arg 45 50 55 gat gee aaa tta atg tta ggc cgt aca ccg aaa age att gca geg att 3424

Asp Ala Lys Leu Met Leu Gly Arg Thr Pro Lys Ser lie Ala Ala He 60 65 70 cgt cct atg aaa gat ggg gtg ate gca gat ttc ttt gtg aca gaa aaa 3472

Arg Pro Met Lys Asp Gly Val lie Ala Asp Phe Phe Val Thr Glu Lys 75 80 85 90 atg ttg caa tat ttt att aaa caa gtg cac age age aat ttt atg cgt 3520

Met Leu Gin Tyr Pha lie Lys Gin Val His Ser Ser Asn Phe Met Arg 95 100 105

cca agt cca cgt gtc tta gtt tgt gta cct geg gga get aeg caa gtc 3568

Pro Ser Pro Arg Val Leu Val Cys Val Pro Ala Gly Ala Thr Gin Val 110 115 120 gaa cga cgt gca ate aaa gaa tet gee att ggt get ggg gca ege gag 3616

Glu Arg Arg Ala lie Lys Glu Ser Ala lie Gly Ala Gly Ala Arg Glu 125 130 135 gtg tac ttg att gag gaa ccg atg geg gca geg att ggt get aaa tta 3664

Val Tyr Leu lie Glu Glu Pro Met Ala Ala Ala lie Gly Ala Lys Leu 140 145 150 cct gtt teg act gee aca ggt teg atg gtg ate gat ate ggt ggt ggt 3712

Pro Val Ser Thr Ala Thr Gly Ser Met Val lie Asp lie Gly Gly Gly 155 160 165 170 aeg aeg gaa gtt geg gtg att tet eta aat ggc att gtg tat tec tet 3760

Thr Thr Glu Val Ala Val He Ser Leu Asn Gly lie Val Tyr Ser Ser 175 180 185 tea gtc ege ate ggt ggt gat cqt ttt gat gag aeg att att tet tat 3808 95- 136852·序列表.doc 1334884

Ser Val Arg lie Gly Gly Asp Arg Phe Asp Glu Ala He lie Ser Tyr 190 195 200 gta cgc aag acg ttc ggt tea att att ggg gaa ccg aca gca gag cgt 3856

Val Arg Lys Thr Phe Gly Ser lie lie Gly Glu Pro Thr Ala Glu Arg 205 210 215 ate aaa caa gag ate ggt agt geg ttt att caa gaa ggc gat gaa gtc 3904 lie Lys Gin Glu lie Gly Ser Ala Phe lie Gin Glu Gly Asp Glu Val 220 225 230 cgt gaa att gaa gtg cat ggt cat aac tta gca gaa ggt geg ccg cgt 3952

Arg Glu lie Glu Val His Gly His Asn Leu Ala Glu Gly Ala Pro Arg 235 240 245 250 tet ttc aaa etc acc tea cgt gat gtg tta gaa get att caa gee ccg 4000

Ser Phe Lys Leu Thr Ser Arg Asp Val Leu Glu Ala lie Gin Ala Pro 255 260 265 tta aat ggc att gtt geg gca gtg cgc acg gee ttg gaa gag tgt caa 4048

Leu Asn Gly lie Val Ala Ala Val Arg Thr Ala Leu Glu Glu Cys Gin 270 275 280 cca gaa cat get geg gat att ttt gaa cgt ggc atg gtc tta act ggt 4096

Pro Glu His Ala Ala Asp lie Phe Glu Arg Gly Met Val Leu Thr Gly 285 290 295 ggc ggt gee ett att cgt aat att gat gtt tta ctg tea aaa gaa acc 4144

Gly Gly Ala Leu lie Arg Asn He Asp Val Leu Leu Ser Lys Glu Thr 300 305 310 ggt gtg ccg gtt ate ate gee gat gat cct tta acc tgt gtt gee cgt 4192

Gly Val Pro Val lie lie Ala Asp Asp Pro Leu Thr Cys Val Ala Arg 315 320 325 330 ggt ggt ggc gag gca tta gag atg ate gat atg cac ggt ggt gat att 4240

Gly Gly Gly Glu Ala Leu Glu Met lie Asp Met His Gly Gly Asp lie 335 340 345 ttt agt gac gat ate taatatgatt taaaagtgcg gtgatattag accgcacttt 4295 Phe Ser Asp Asp lie 350

taettetett ttattgctga caaggctagc etaattegta tatgaaacct atttttggaa 4355 aagcacctcc tttaggtctt egettaatte tggcgatttt agcatccatt gcattg.attg 4415 ttteggaegg tcaatccaat gegatgatta aagcacgcag tattatggaa accgcagtag 4475 gcgggctgta ttatettgee aatacaccga gaacggtatt ggatggggtt tcagataatt 4535 tggttgatac caataaattg caaattgaaa accgagtttt gcgtgatcaa ctgcgtgaaa 4595 aaaatgcaga tttattgttg ttagatcaac tcaaagtaga aaatcaacgc ctgcgcttat 4655 tgettaatte ccctctacgt acagatgagt ataaaaaaat tgctgaagtt ttaacggcag 4715 aaactgatgt gtategtaag caagtcgtga ttaaccaagg acaacgtgac ggtgcttatg 4775 tcgggcagcc gattattgat gaaaagggta ttgtcgggca acttatctcc gttggtgaaa 4835 ataegagteg cgttcttcta ttgacagatg tgactcattc tattccagta caagtactac 4895 -96- Π6852·序列表.doc

l SI 1334884 gtaatgatgt ccgtttgatt gctagtggaa caggacggaa tgatgaactg agtttagatc 4955 atgtgccgcg ttcggtcgat attgtcaaag gggatttatt agtcacttct ggattaggtg 5015 ggcgtttttt agaaggttat cctgttgcca ttgtggaatc cgtatcacgt gatgggcaaa 5075 attattttgc tactgtaaca gcaaagccat tagcttcgat tgaacgttta cgctatgttt 5135 tgcttttatg gccgacgaat gaagagatgc gcaaagtcca gtctatttca cctgca 5191

<210> 42 <211> 351 <212>蛋白質 <213>敗血性巴斯德桿SI <400> 42

Met Leu Phe Lys Lys lie Arg Gly Leu Phe Ser Asn Asp Leu Ser lie 15 10 15

Asp Leu Gly Thr Ala Asn Thr Leu lie Tyr Val Lys Gly Gin Gly lie 20 25 30

Val Leu Asp Glu Pro Ser Val Val Ala lie Arg Gin Glu Arg Ser Gly 35 40 45

Ala Leu Lys Ser lie Ala Ala Val Gly Arg Asp Ala Lys Leu Met Leu 50 55 60

Gly Arg Thr Pro Lys Ser lie Ala Ala lie Arg Pro Met Lys Asp Gly 65 70 75 80

Val lie Ala Asp Phe Phe Val Thr Glu Lys Met Leu Gin Tyr Phe lie 85 90 95

Lys Gin Val His Ser Ser Asn Phe Met Arg Pro Ser Pro Arg Val Leu 100 105 110

Val Cys Val Pro Ala Gly Ala Thr Gin Val Glu Arg Arg Ala lie Lys 115 120 125

Glu Ser Ala He Gly Ala Gly Ala Arg Glu Val Tyr Leu lie Glu Glu 130 135 140

Pro Met Ala Ala Ala lie Gly Ala Lys Leu Pro Val Ser Thr Ala Thr 145 150 155 160

Gly Ser Met Val lie Asp lie Gly Gly Gly Thr Thr Glu Val Ala Val 165 170 175 lie Ser Leu Asn Gly He Val Tyr Ser Ser Ser Val Arg lie Gly Gly 180 185 190 ^

Asp Arg Phe Asp Glu Ala lie lie Ser Tyr Val Arg Lys Thr Phe Gly 195 200 205

Ser lie lie Gly Glu Pro Thr Ala Glu Arg lie Lys Gin Glu lie Gly 210 215 220

Ser Ala Phe lie Gin Glu Gly Asp Glu Val Arg Glu He Glu Val His 225 230 235 240

Gly His Asn Leu Ala Glu Gly Ala Pro Ara Ser Phe I.ys Leu Thr Ser •97· 136852·序列表.doc I Si 1334884 245 250 255

Arg Asp Val Leu Glu Ala lie Gin Ala Pro Leu Asn Gly lie Val Ala 260 265 270

Ala Val Arg Thr Ala Leu Glu Glu Cvs Gin Pro Glu His Ala Ala Asp 275 260 285 lie Phe Glu Arg Gly Met Val Leu Thr Gly Gly Gly Ala Leu lie Arg 290 295 300

Asn lie Asp Val Leu Leu Ser Lys Glu Thr Gly Val Pro Val lie lie 305 310 315 320

Ala Asp Asp Pro Leu Thr Cys Val Ala Arg Gly Gly Gly Glu Ala Leu 325 330 335

Glu Met lie Asp Met His Gly Gly Asp lie Phe Ser Asp Asp lie 340 345 350

<210> 43 <211> 2172 <2.12 > DNA <213>敗血性巴斯德桿菌 <220> <221> CDS <222> (1) . . (1464) <220> <223> pnp <400> 43 acc cgc gtt ggg att ggg tgg cac ctg aac cca aat acc gcg tta att 48

Thr Arg Val Gly lie Gly Trp His Leu Asn Pro Asn Thr Ala Leu He . 1 5 10 15 gaa aaa gtg aaa gcg att gca gaa gcg cgt tta ggc gaa gca tac cgt 96

Glu Lys Val Lys Ala lie Ala Glu Ala Arg Leu GXy Glu Ala Tyr Arg 20 25 30 ate act gaa aac aag cac gtt atg aac aaa att gat gcg att aaa get 144

lie Thr Glu Asn Lys His Val Met Asn Lys lie Asp Ala He Lys Ala 35 40 45 gat gtg att gca caa ate aca get gaa gta gca gaa ggc gaa gac at.c 192

Asp Val lie Ala Gin He Thr Ala Glu Val Ala Glu Gly Glu Asp lie 50 55 60 agt gaa ggg aaa att gtc gat att ttc acc gca ett gaa age caa ate 240

Ser Glu Gly Lys lie Val Asp lie Phe Thr Ala Leu Glu Ser Gin lie 65 70 75 80 gta cgt age cgt ate att get ggt gaa cca cgt att gat ggt cgt aca 288

Val Arg Ser Arg lie lie Ala Gly Glu Pro Arg lie Asp Gly Arg Thr 85 90 95 gtg gat act gtt cgt gca tta gat att tgt act ggt gtt tta cca cgt 336

Val Asp Thr Val Arg Ala Leu Asp lie Cys Thr Gly Val Leu Pro Arg 100 105 110 aca cac ggt tet gcg att ttc acc cgt gqt gaa aca cag gca tta act 384 • 98 · 136852-序列表.doc 151 1334884

Thr His Gly Ser 115 Ala lie Phe Thr 120 Arg Gly Glu Thr Gin 155 Ala Leu Ala gtc geg aca tta ggt aca gaa cgt qat gca caa aft att gat gaa tna 432 Val Ala 130 Thr Leu Gly Thr Glu 135 Arg Asp Ala Gin lie 140 lie Asp Glu Leu aca QQt gag cgt tea gat cac ttc tta ttc cac aac ttc ccg c〃a 480 Thr 145 Gly Glu Arg Ser Asp 150 His Phe Leu Phe His 155 Tyr Asn Phe Pro Pro 160 tat tet gtg ggt gaa acc ggt atg att ggt tea cca aaa cgt cgt gaa 528 Tyr Ser Val Gly Glu 165 Thr Gly Met lie Gly 170 Ser Pro Lys Arg Arg 175 Glu att ggt cat ggt cgt tta geg aaa ege ggt gta get gca gtg atg cca 576 lie Gly His Gly Arg 180 Leu Ala Lys Arg 185 Gly Val Ala Ala Val 190 Met Pro aca ett gee gag ttc ccg tat gtg gta cgt gtt gtc tet gaa ate aca 624 Thr Leu Ala 195 Glu Phe Pro Tyr Val 200 Val Arg Val Val Ser 205 Glu lie Thr gaa tea aat ggt tet tet tet atg gca teg gtt tgt ggt geg tet tta 672 Glu Ser 210 Λ8Π Gly Ser Ser Ser 2X5 Met Ala Ser Val Cys 220 Gly Ala Ser Leu gca tta atg gat geg ggt gta cca att aaa geg geg gtt gca ggt att 720 Ala 225 Leu Met Asp Ala Gly Val 230 Pro lie Lys Ala 235 Ala Val Ala Gly lie 240 gca atg ggc tta gtc aaa gaa gac gaa aaa ttt gtg gtg ett tea gac 768 Ala Met Gly Leu Val 245 Lys Glu Asp Glu Lys 250 Phe Val Val Leu Ser 255 Asp ate tta ggt gat gaa gat cac tta ggt gac atg gac ttc aaa gtc geg 816 lie Leu Gly Asp 260 Glu Asp His Leu Gly Asp 265 Met Asp Phe Lys 270 Val Ala ggt aca cgt aeg ggt gtg aeg gca tta caa atg gat ate aaa ate gaa 864 Gly Thr Arg 275 Thr Gly Val Thr Ala 280 Leu Gin Met Asp lie 285 Lys lie Glu ggt ate aca gca gaa ace atg caa att geg tta aac caa geg aaa age 912 Gly lie 290 Thr Ala Glu lie Met 295 Gin lie Ala Leu Asn 300 Gin Ala Lys Ser gca cgt tta cac att tta ggt gtg atg gag caa geg ate cca geg cca 960 Ala 305 Arg Leu His lie Leu 310 Gly Val Met Glu Gin 315 Ala lie Pro Ala Pro 320 cgt geg gat att tet gat ttt gca ccg cgt att tac act atg aaa att 1008 Arg Ala Asp lie Ser 32S Asp Phe Ala Pro Arg 330 lie Tyr Thr Met Lys 335 lie gat ccg aag aaa ate aaa gat gtg ate ggt aaa ggt ggt gca acc att 1056 Asp Pro Lys Lys 340 lie Lys Asp Val lie 345 Gly Lys Gly Gly Ala 350 Thr lie cgt gee tta aca gaa gaa aca ggt acc tea att gat ate gat gat gat 1104 Arg Ala Leu 355 Thr Glu Glu Thr Gly 360 Thr Ser lie Asp lie 365 Asp Asp Asp 136852-序列表.doc -99- 1334884 ggc acg gtg aag att get gcg get gat ggc aat tea gca aaa gag gtg 1152

Gly Thr Val Lys He Ala Ala Val Asp Gly Asn Ser Ala Lys Glu Val 370 atg geg cgt att gaa gat att act gca gaa gtt gaa geg ggt gca gtg 1200

Met Ala Arg lie Glu Asp He Thr Ala Glu Val Glu Ala Gly Ala Val 385 390 395 400 tac aaa ggt aaa gtt act cgt tta get gat ttt ggt gee ttc gtt tet 1248

Tyr Lys Gly Lys Val Thr Arg Leu Ala Asp Phe Gly Ala Phe Val Ser 405 410 4X5 ate gca ggt aac aaa gaa ggc tta gtg cat att tet caa ate geg gaa 1296 lie Val Gly Asn Lys Glu Gly Leu Val His lie Ser Gin He Ala Glu 420 425 430 gag cgt gtt gag aaa gtg agt gat tat ett gca gtg ggg caa gaa gtg 1344

Glu Arg Val Glu Lys Val Ser Asp Tyr Leu Ala Val Gly Gin Glu Val 435 440 445

act gtt aaa gtg gtt gag att gat cgt caa ggt cgt att cgt tta acc 1392

Thr Val Lys Val Val Glu He Asp Arg Gin Gly Arg lie Arg Leu Thr 450 455 460 atg aaa gaa gtt gca cca aag caa gaa cac gtt gat tet gtt gtc gca 1440

Met Lys Glu Val Ala Pro Lys Gin Glu His Val Asp Ser Val Val Ala 465 470 475 480 gac gtt gee gca gaa gaa aac gca taagcaacaa acaccaacgc ccttcgtgat 1494 Asp Val Ala Ala Glu Glu Asn Ala 465 aaagggcgtt ggtgtgcatg ttgataagta caatttgtgc tttaaggega agcgaaatga 1554 agcaatttca tccgtggtta aagtgcctgc taattttccc attttgggta tgttgtttaa 1614 cagcttgtgt taatcatgaa caagtttttc tttcaaaaga gaaattaatg ttageagage 1674 aacatccgaa tgatcatctt gageatgagg tgatggttgc gcaaattagc gaattgttac 1734 ttgttaaagg gctaaaaaaa gaagaacgtg cgattttaca ttttgagcga ggcgtgctgt 1794

aegatagett aggattgtgg gcattggcgc gttatgattt tgaccaaaca ttagcgttgt 1854 atccaaagtt ggcagcagcg tttaattatt taggtttata tttattgtta gaggaagatt 1914 acagcgcatc tetagatate tttaatgtgt tgtttgaact tgatcctcaa tatgagtatg 1974 cattcctaaa tagagggeta aatttttatt aegteggaeg ttatgaatta gctcagcggg 2034 attttcttca attttatcaa gccgataaat cagatccata ccgcacttta tggctttatt 2094 taaatgaatt aaagcataat cctcaggatg cttctaaaaa tcttgctcaa cgagcaatgg 2X54 ggctttctga tgaatatt 2172 <210> 44 <211> 488 <212>蛋白質 <213>敗血性巴斯德桿菌 <400> 44 136852·序列表.doe -丨〇〇-

Tiir* Arg Va丄 GJLy I丄e Gly Trp His Leu Asn Ργό Asn Thr Ala Leu He 15 10 15

Glu Lys Val Lys Ala lie Ala Glu Ala Arg Leu Gly Glu Ala Tyr Arg 20 25 30 lie Thr Glu Asn Lys His Val Met Asn Lys lie Asp Ala lie Lys Ala 35 40 45

Asp Val lie Ala Gin lie Thr Ala Glu Val Ala Glu Gly Glu Asp lie 50 55 60

Ser Glu Gly Lys lie Val Asp lie Phe Thr Ala Leu Glu Ser Gin lie 65 70 75 60

Val Arg Ser Arg lie lie Ala Gly Glu Pro Arg lie Asp Gly Arg Thr 85 90 95

Val Asp Thr Val Arg Ala Leu Asp lie Cys Thr Gly Val Leu Pro Arg 100 105 110

Thr His Gly Ser Ala lie Phe Thr Arg Gly Glu Thr Gin Ala Leu Ala 115 120 125

Val Ala Thr Leu Gly Thr Glu Arg Asp Ala Gin lie He Asp Glu Leu 130 135 140

Thr Gly Glu Arg Ser Asp His Phe Leu Phe His Tyr Asn Phe Pro Pro 145 150 155 160

Tyr Ser Val Gly Glu Thr Gly Met lie Gly Ser Pro Lys Arg Arg Glu 165 170 175 lie Gly His Gly Arg Leu Ala Lys Arg Gly Val Ala Ala Val Met Pro 180 185 190

Thr Leu Ala Glu Phe Pro Tyr Val Val Arg Val Val Ser Glu lie Thr 195 200 205

Glu Ser Asn Gly Ser Ser Ser Met Ala Ser Val Cys Gly Ala Ser Leu 210 215 220

Ala Leu Met Asp Ala Gly Val Pro lie Lys Ala Ala Val Ala Gly lie 225 230 235 240

Ala Met Gly Leu Val Lys Glu Asp Glu Lys Phe Val Val Leu Ser Asp 245 250 255 lie Leu Gly Asp Glu Asp His Leu Gly Asp Met Asp Phe Lys Val Ala 260 265 270

Gly Thr Arg Thr Gly Val Thr Ala Leu Gin Met Asp lie Lys lie Glu 275 280 285

Gly He Thr Ala Glu He Met Gin He Ala Leu Asn Gin Ala Lys Ser 290 295 300

Ala Arg Leu His lie Leu Gly Val Met Glu Gin Ala lie Pro Ala Pro 305 310 315 320

Arg Ala Asp lie Ser Asp Phe Ala Pro Arg lie Tyr Thr Met Lys lie 325 330 335 -101 - 136852·序列表.doc

I SI 1334884

Asp Pro Lys hys I丄e Lys Asp VaU lie GJy bys Gly Gly Axa Thr lie 340 345 350

Arg Ala Leu Thr Glu Glu Thr Gly Thr Ser He Asp lie Asp Asp Asp 355 360 365

Gly Thr Val Lys lie Ala Ala Val Asp Gly Asn Ser Ala Lys Glu Val 370 375 380

Met Ala Arg lie Glu Asp lie Thr Ala Glu Val Glu Ala Gly Ala Val 385 390 395 400

Tyr Lys Gly Lys Val Thr Arg Leu Ala Asp Phe Gly Ala Phe Val Ser 405 410 415 lie Val Gly Asn Lys Glu Gly Leu Val His lie Ser Gin lie Ala Glu 420 425 430

Glu Arg Val Glu Lys Val Ser Asp Tyr Leu Ala Val Gly Gin Glu Val 435 440 445

Thr Val Lys Val Val Glu He Asp Arg Gin Gly Arg lie Arg Leu Thr 450 455 460

Met Lys Glu Val Ala Pro Lys Gin Glu His Val Asp Ser Val Val Ala 465 470 475 480

Asp Val Ala Ala Glu Glu Asn Ala 485

<210> 45 <211> 633 <212> DNA <213>敗血性巴斯德样菌 <220> <221> CDS <222> (2)..(631) <220> <223> purF <400> 45 c gat ggg gtt tct gtt tat get gcc cgt gtt cat atg gga caa cgt tta Asp Gly Val Ser Val Tyr Ala Ala Arg Val His Met Gly Gin Arg Leu 1 5 10 15 ggt gaa aaa att gca egg gaa tgg geg gat gtg gat gat att gat gtg Gly Glu Lys lie Ala Arg Glu Trp Ala Asp Val Asp Asp lie Asp Val 20 25 30 gtc att ect gtg cct gaa acc tct aac gat att get tta cgt ate geg Val lie Pro Val Pro Glu Thr Ser Asn Asp lie Ala Leu Arg lie Ala 35 40 45 ege gtg tta aat aaa ccg tat cgt caa ggt ttt gtg aaa aat ege tat Arg Val Leu Asn Lys Pro Tyr Arg Gin Gly Phe Val Lys Asn Arg Tyr 50 55 60 gta gga cgt aeg ttt att aeg ccg ggg cag gca ttg ega gtc agt tct Val Gly Arg Thr Phe lie Met Pro Gly Gin Ala Leu Arg Val Ser Ser 65 70 75 80 49 97 145 193 102· 136852-序列表.doc 241 gtt aga cgc aaa ccc aac acc act get tea gaa ttt aaa gat aag aat 289

Val Arg Arg Lys Leu Asn Thr lie Ala Ser Glu Phe I>ys Asp Lys Asn as 90 95 gtg tta tta gtt gac gac teg act gta cgt ggt acc aeg tet gaa caa 337

Val Leu Leu Val Asp Asp Ser lie Val Axg Gly Thr Thr Ser Glu Gin 100 105 1X0 att gtc gaa atg geg aga geg gca ggt geg aag aaa att tat ttt gee 385 lie Val Glu Met Ala Arg Ala Ala Gly Ala Lys Lys lie Tyr Phe Ala 115 120 125 tet get gca cca gaa att cgt tat cca aat gtg tat ggt att gat atg 433

Ser Ala Ala Pro Glu lie Arg Tyr Pro Asn Val iyr Gly lie Asp Met 130 135 140 cca acc aaa aat gaa ttg ate get tat ggt cgt gat gta gat gaa att 481

Pro Thr Lys Asn Glu Leu He Ala Tyr Gly Arg Asp Val Asp Glu lie 145 150 155 160 get aac tta att ggt gtg gat aaa ttg att ttc caa gat ttg gat geg 529

Ala Asn Leu lie Gly Val Asp Lys Leu lie Phe Gin Asp Leu Asp Ala 165 170 175 tta act ggt tet gtg caa caa gaa aat cca agt att caa gac ttt gat 577

Leu Thr Gly Ser Val Gin Gin Glu Asn Pro Ser lie Gin Asp Phe Asp 180 185 190 tgt teg gtg ttt aca ggg gtt tat gtg aeg ggc gat att aca cct gaa 625

Cys Ser Val Phe Thr Gly Val Tyr Val Thr Gly Asp lie Thr Pro Glu 195 200 205 tat ctg ga 633

Tyr Leu 210 <210> 46 <211> 210 <212>蛋白質 <213>敗血性巴斯德桿菌 <400> 46

Asp Gly Val Ser Val Tyr Ala Ala Arg Val His Met Gly Gin Arg Leu 15 10 15

Gly Glu Lys lie Ala Arg Glu Trp Ala Asp Val Asp Asp lie Asp Val 20 25 30

Val lie Pro Val Pro Glu Thr Ser Asn Asp lie Ala Leu Arg lie Ala 35 40 45

Arg Val Leu Asn Lys Pro Tyr Arg Gin Gly Phe Val Lys Asn Arg Tyx 50 55 60

Val Gly Arg Thr Phe lie Met Pro Gly Gin Ala Leu Arg Val Ser Ser 65 70 75 80

Val Arg Arg Lys Leu Asn Thr lie Ala Ser Glu Phe Lys Asp Lys Asn 85 90 95

Val Leu Leu Val Asp Asp Ser lie Val Arg Gly Thr Thr Ser Glu Gin aoo i〇5 no 136852-序列表.doc -103- lie Val Glu Met Ala Arg Ala Ala Gly Ala Lys Lys lie Tyr Phe Ala 115 120 125

Ser Ala Ala Pro Glu lie Arg Tyr Pro Asn Val Tvr Gly He Asd Met 130 135 340

Pro Thr Lys Asn Glu Leu lie Ala Tyr Gly Arg Asp Val Asp Glu He 145 150 155 150

Ala Asn Leu lie Gly Val Asp Lys Leu lie Phe Gin Asp Leu Asp Ala 165 170 ' 175

Leu Thr Gly Ser Val Gin Gin Glu Asn Pro Ser lie Gin Asp Phe Asp 180 185 190

Cys Ser Val Phe Thr Gly Val Tyr Val Thr Gly Asp lie Thr Pro Glu 195 200 205

Tyr Leu 210

<210> 47 <211> 4788 <212> DNA <213>敗血性巴斯德桿菌 <220> <221> CDS <222> (1) . . (876) <220> <223> rci <220>

«223>在位置挪4處的核甘唤是Α、·Γ'ϋ或C <400> 47 gac gag gag aga aaa tta get gat ttg gca aaa ggt ate get cca gat 4Θ

Asp Glu Glu Arg Lys Leu Ala Asp Leu Ala Lys Gly lie Ala Pro Asp 15 10 15 att att ttt aga gat gta ata gaa ege tat caa aat gaa gtg tet ata 96 lie lie Phe Arg Asp Val lie Glu Arg Tyr Gin Asn Glu Val Ser lie 20 25 30 act aaa aaa ggc geg ega aat gaa att ata aga tta aac ege ttt tta 144

Thr Lys Lys Gly Ala Arg Asn Glu lie lie Arg Leu Asn Arg Phe Leu 35 40 45 aga tat gat att tet aat ctg tat att cgt gat tta aga aaa gaa gat 192

Arg Tyr Asp lie Ser Asn LeU Tyr lie Arg Asp Leu Arg Lys Glu Asp 50 55 60 ttt gag gag tgg ate aga att ege eta acc gaa gta teg gat get age 240

Phe Glu Glu Trp lie Arg lie Arg Leu Thr Glu Val Ser Asp Ala Ser 65 70 75 80 gtt aga cgt gag ett gtt act ata teg tea gtg ctg aca aca gca ata 288

Val Arg Arg Glu Leu Val Thr lie Ser Ser Val Leu Thr Thr Ala lie 85 90 95 aat aag tgg gga tat att tea agg cat cca atg act ggt att gaa aaa 336 136852-序列表.doc - 104- IS] 1334884

Asn Lys Trp Gly Tyr lie Ser Arg His Pro Met Thr Gly lie Glu Lys 100 105 110 cca aaa aac teg gca gaa aga aaa gaa ega tat tea gaa cag gac att 384

Pro Lys Asn Ser Ala Glu Arg Lys Glu Arg Tyr Ser Glu Gin Asp lie 115 120 125 aaa aca ata tta gaa aca get: aga tat tgt gaa gat aaa eta ccc ata 432

Lys Thr lie Leu Glu Thr Ala Arg Tyr Cys Glu Asp Lys Leu Pro lie 130 135 140 aca etc aaa caa aga gta gca att gca atg tta ttt get att gaa acc 480

Thr Leu Lys Gin Arg Val Ala lie Ala Met Leu Phe Ala lie Glu Thr 145 150 155 160 get atg cgt get ggt gag att get agt ata aaa tgg gat aat gtt ttt 528 hla Met Arg Ala Gly Glu He Ala Ser lie Lys Trp Asp Asn Val Phe 165 170 175

ett gaa aag aga ata gta cat tea ccg aca act aaa aac ggg cac tet 576

Leu Glu Lys Arg lie Val His Leu Pro Thr Thr Lys Asn Gly His Ser 180 185 190 aga gat gtg ccg ett teg caa aga get gtt geg eta att tta aaa atg 624

Arg Asp Val Pro Leu Ser Gin Arg Ala val Ala Leu lie Leu Lys Met 195 200 205 aaa gag gta gaa aat gga gat ett gtg ttt cag acc aeg cct gaa tea 672

Lys Glu Val Glu Asn Gly Asp Leu Val Phe Gin Thr Thr Pro Glu Ser 210 215 220 tta age acc aeg ttt aga gtg tta aag aaa gag tgt gga ett gaa cat 720

Leu Ser Thr Thr Phe Arg Val Leu Lys Lys Glu Cys Gly Leu Glu His 225 230 235 240 etc cat ttt cat gat aeg aga agg gaa geg ttg aeg aga tta tet a.ag 768

Leu His Phe His Asp Thr Arg Arg Glu Ala Leu Thr Arg Leu Ser Lys 245 250 255 aaa gta gat gta atg act eta gee aaa att age gga cat aga gat tta 816

Lys Val Asp Val Met Thr Leu Ala Lys lie Ser Gly His Arg Asp Leu

260 265 270 aga att tta caa aac aca tat tac gca ccg aat atg agt gaa gtg gca Θ64

Arg lie Leu Gin Asn Thr Tyr Tyr Ala Pro Asn Met Ser Glu Val Ala 275 280 285 aac ttg ttg gat taattcactc ttcttaaata cgccttttgc cacttgatta 916

Asn Leu Leu Asp 290 catcgccagc ettatatett ttactttcat tacttccttt ttctaatgaa actggggatg 976 gaaagtcttg gcgggtaata atatgaegag atgtgtaatt gtaagaaega ttaatcatga 1036 tagaaatgtc ttcaatacta agaagaactg gactatcttc tttaagttga gctaaggctc 1096 caaacctcac agagegtage acttcatctt gttgtttctc tgttagagaa atattttcca 1156 tttttacctc acttaaataa aaaagccgtc atggaegget taataaagca tttegetata 1216 cagttctttt gaggtgtacc caatatcgaa cttqatttga ttgttttgtt ttgcgatttc 1276 -105- 136852·序列表.doc 1334884 cagtgcttct tttcgatcaa caaatcttcc gaagttggta ataacaccct gtttcttacc 1336 aattagcgga tattgtgtac cgttctcaat agatttaatt tgtttacgca taaacatgtc 1396 atagtgtctc gcgccagtca ctaaatgaag ttcttttgtt gtgtaatctt caagctcgca 1456 cgcagcgcag acgattaatt ctggacgatc tcccattttc ctatctccga gcatttttca 1516 tagcctcaaa ccaagcttgt gcgtctcctt cactgtaaaa taatgaaccg ctttctagca 1576 tttttctgtg cttatctcta ttagcatcaa aatagaatct cataattgtt gagttacccc 1636 ttttaaagac ctcttgattg tggtgtgctg ttttgattgg agcgggcaaa gtaagcgtta 1696 ctgttgggcg tggttcttcc cacattccga ctatatcaaa tggattttct aaatgcggat 1756 atttgttatg ttcaaaaaaa gaagatccat caatcgtcca agaatttctc tcaaattgtg 1816 tcttgctctt gccaatttga aatacacctt gatataagaa agtgttgaac ggaaagaact 1876 ttgaataatc agcagttaca tatcctttac ttccatttct aagcacaact ggctcaccgg 1936 ttaatgcttt ttctaagtca aatgctttca ttttttactc tccagcttgc tcatcaataa 1996 gctcatcaat ccattctcga atttcagatt ggaaattttc taacgaatta ttttcattaa 2056 aataatctgc ttcttttaac ttactcaccg ctgttttact aaagcattta taaaaaagac 2116 gcttttcttc aaacaaatca tcttttgaga ttgcgcatac ttttcctacg ccctctatac 2176 ttttgtcttg aataaaacta ttaaaaagaa cctcttcttg ctgcttgttt tctactaaaa 2236 tagaaatcgt atattttttt ggatattcca tttttaatcc tttcttttag ataacaaaaa 2296 accgcatttc tgcggttatt ctgtgtattt atttaaaata ttacttatag tttcagcatc 2356 ttctaatgtt aatttagaat agcttgaggt catttttcct ttaacggcaa tcctcaattc 2416 ttccagcttt aagctataca agtagtcttt tttatcttta taaatacgac cgtacaccat 2476 tccggataat ttaccttctt catacccatt agataaattg atctttctat ctcgaccgcg 2536 cactctaatt gttaaatatt ttcttccaat ttttaagact tcagcctcat gttcaatgtt 2596 tgagcgcatg agtgtttcta aaaaatatac ggtatcgcca actttaagat ttttaatcca 2656 atctttattc ataaataccc ttatactttc ggtggcagtg gaagtggttg ccaatgagtt 2716 actgaagata gatggtaagt acgaagagac ataaagaaaa catcatctcg ccacgcgatt 2776 tttatttccc ctctatctgt ataaatcaga atatcctctc tctcactggg caatctatct 2036 tttacactaa tccagccatc atcttgcgaa aattccacaa tttctggctt ttcaagcacc 2896 aaatcagagc cgtaatcttc tttttcctct tcgctcaact gcttttcctt gcagtcagcc 2956 ttgccaagga cgacaccgta cacggcataa ggtaaatcat tcaacgcaaa atcctcatga 3016 tcacacatat catctgcgaa ctcatgagct tctactgcac catctaaaca actttgctta 3076 gcttgatnta atgtttcgtg taagctaacg atatgtatgt cattagatac atctactgag 3136 aaaaattttt tttccgttcc tatcatagtt aaattcctta ttcttttatt aatgggcgag 3196 • 106- 136852-序列表.doc i si 1334884

gaatcggttg ccagtgtgta acgttaatag ctagaticata aattggacca ttctgattta 3256 tagctaccca aaaatattca ccatcagcaa tttctcttga tgcaattgcg taatgctcgc 3316 catattcacc ttcacaaaaa gcaatgactg gtgtttcaac ttctggtaac ttgtcatcaa 3376 cactaatcca gttgctatat tcccaatgaa aattaagctt ctcatagcca ttgatgactt 3436 gatctaacgc aggtcttaat tgggtttctc caacaataat atgtgttgtt atcttaatct 3496 ctgggattcc atctattact tcacaccagt ttttttcaat aaagtccaat ctttttttat 3556 ctgctaaaag actttcatat tctgcttttg tgattgttac tgtttctgtc atagttaaat 3616 tcctcatgat aaattccatg tttatttacc ttttttctgg cacaaaaaaa cacgctattg 3676 cgtgctgtct tgattgatat aatcaattaa tctaagccca atccatttca taactggaac 3736 agccatactg tttccaactg ctttgtatcg tggagaatca gggcattcat ctattgattt 3796 gttgcgatat gggattttag tgtaatcgtc tggaaatccc tgtaatcttt cacactcacg 3856 cggcgttagt tttctaacaa ctgattctac tgataaagca acgcaaggaa cattattacc 3916 accagttccc attcttgctt ttaaggttgg tgaaatatca tcgtgaatac gacaagcttc 3976 ttcaccctta acctcaaata gaatattttc caatcctcca tttcttccaa tgcaatgcgc 4036 tgtattttta gaaatgattg gatcttgtga accgtgaaca acaaaagttt cactaccgcc 4096 agcaagaact ccaccgcttg ctcttaatgt tcccgcaaca tcggatttgc gatattgagc 4156 aaagcttgtc tcaataaagg cggcaatatc ttttgcctgc gttccgctct gttcaatatt 4216 ccctcgcacg cttttggact caatgagtat ttgtgcaaca cttcgttttc tagcacttgc 4276 cacaagaaac actcttttac gacgttgggc aactccgaag tattgagcat cgagaactcg 4336 ccagcagatt gttcggattg aatgcacata accagcgttc gtccatcttc tccctgtgtg 4396 ctgcaatggc tcacactctt gaaccagtcc agccagaagg tgtccgaatg cgttgtccga 4456 ggtggatagc acacccggaa cgttttccca cacgagaatg cacggtggtt tgttgtcatt 4516 gaatctaaca tagtcgatcg cttctaaaat tttaattaaa actaacgtga gatttcctcg 4576 ctcatcgtct aaactttgac gattgccagc aactgaaaaa gattgacaag gagtaccacc 4636 aactaaaacg tctggtgccg gaatttcacg atctaatatt ttctgttgca attcagtcat 4696 atcaccaaga ttgggaacgt tagggtaatg gtaagctaat actgcgcttg ggaattgctc 4756 aatttcagaa aaccagagtg gctctgcgaa tt 4788 <210> 48 <211> 292 <212>蛋白質 <213>敗血性巴斯德桿菌 <400> 48

Asp Glu Glu Arg Lys Leu Ala Asp Leu Ala Lys Gly lie Ala Pro Asp 1 '5 10 15 •107· 136852·序列表,doc 1334884 lie lie Phe Arg Asp Val He Glu Arg Tyr Gin Asn Glu Val Ser lie 20 25 30

Thr Lys Lys Gly Ala Axg Asn Glu He lie Arg Le» Asn Arg Phe Leu 35 40 45

Arg Tyr Asp He Ser Asn Leu Tyr lie Arg Asp Leu Arg Lys Glu Asp 50 55 60

Phe Glu Glu Trp lie Arg lie Arg Leu Thr Glu Val Ser Asp Ala Ser 65 70 75 80

Val Arg Arg Glu Leu Val Thr lie Ser Ser Val Leu Thr Thr Ala He 85 90 95

Asn Lys Trp Gly Tyr lie Ser Arg His Pro Met Thr Gly lie Glu Lys 100 105 110

Pro Lys Asn Ser Ala Glu Arg Lys Glu Arg Tyr Ser Glu Gin Asp lie 115 120 125

Lys Thr He Leu Glu Thr Ala Arg Tyr Cys Glu Asp Lys Leu Pro lie 130 135 140

Thr Leu Lys Gin Arg Val Ala lie Ala Met Leu Phe Ala lie Glu Thr 145 150 155 160

Ala Met Arg Ala Gly Glu lie Ala Ser lie Lys Trp Asp Asn Val Phe 165 170 175

Leu Glu Lys Arg lie Val His Leu Pro Thr Thr Lys Asn Gly His Ser 180 1Θ5 190

Arg Asp Val Pro Leu Ser Gin Arg Ala Val Ala Leu lie Leu Lys Met 195 200 205

Lys Glu Val Glu Asn Gly Asp Leu Val Phe Gin Thr Thr Pro Glu Ser 210 215 220

Leu Ser Thr Thr Phe Arg Val Leu Lys Lys Glu Cys Gly Leu Glu His 225 230 235 240

Leu His Phe His Asp Thr Arg Arg Glu Ala Leu Thr Arg Leu Ser Lys 245 250 255

Lys Val Asp Val Met Thr Leu Ala Lys lie Ser Gly His Arg Asp Leu 260 265 270

Arg lie Leu Gin Asn Thr Tyr Tyr Ala Pro Asn Met Ser Glu Val Ala 275 280 28S

Asn Leu Leu Asp 290

<210> 49 <211> 1618 <212> DNA <213>敗血性巴斯德样菌 <220> <221> CDS <222> (2) . . (1195) -108- 136852-序列表.doc 1334884 <22ϋ> <223> S〇pE <400> 49 9 99C gac eta tgt ctg aaa ata tet aca tgg tgt caa agt cac aga ate 49 Gly Asp L»eu Cys Leu Lys He Ser Thr Trp Cys Gin Ser His Arg lie 15 10 15 aat caa gca att ege aca att caa agt eta tea acc gca gtc ate ggt

Asn Gin Ala lie Arg Thr lie Gin Ser Leu Ser Thr Ala Val lie Gly 20 25 30 ate gtc tgt act gca aat gac gca gac aat gaa aca ttc cca etc aat lie Val Cys Thr Ala Asn Asp Ala Asp Asn Glu Thr Phe Pro Leu Asn 35 40 45 gaa ccc gtt etc ate aca aac gtg gca geg gca att ggc aag get gga

Glu Pro Val Leu lie Thr Asn Val Ala Ala Ala lie Gly Lys Ala Gly 50 55 60

aaa caa ggc aeg ett tea cgt geg ett gac ggg att tet gat gta gtc

Lys Gin Gly Thr Leu Ser Arg Ala Leu Asp Gly lie Ser Asp Val Val 65 70 75 80 aat tgc aaa gtg att gtt gtg ega gtg caa gaa agt geg caa gaa gac

Asn Cys Lys Val lie Val Val Arg Val Gin Glu Ser Ala Gin Glu Asp 85 90 95 gaa gaa aca aaa gca agt gaa atg aac aeg gca att att ggc aca ate

Glu Glu Thr Lys Ala Ser Glu Met Asn Thr Ala lie lie Gly Thr lie 100 105 110 aca gaa gaa ggg cag tac aca ggc ttg aag geg tta ttg att geg aaa

Thr Glu Glu Gly Gin Tyr Thr Gly Leu Lys Ala Leu Leu lie Ala Lys 115 120 125 aac aaa ttc ggt ate aaa cca cgt att tta tgt gtg cca aaa ttc gac

Asn Lys Phe Gly lie Lys Pro Arg lie Leu Cys Val Pro Lys Phe Asp 130 135 140 aca aaa gaa gtc gee aca gag ett gca agt ate gee gee aaa etc aac

Thr Lys Glu Val Ala Thr Glu Leu Ala Ser lie Ala Ala Lys Leu Asn

97 145 193 241 289 337 385 433 481 529 577 625 673 145 150 155 160 gca ttt get tac att tea tgt caa ggg tgt aaa aeg aaa gaa caa geg

Ala Phe Ala Tyr lie Ser Cys Gin Gly Cys Lys Thr Lys Glu Gin Ala 165 170 175 gtg caa tat aaa ege aac ttc tea caa cgt gaa gtc atg ctg ate atg

Val Gin Tyr Lys Arg Asn Phe Ser Gin Arg Glu Val Met Leu lie Met 180 185 190 ggc gat ttt ctg tea ttt aat gtc aac aca tea aaa gtt gag att gac

Gly Asp Phe Leu Ser Phe Asn Val Asn Thr Ser Lys Val Glu lie Asp 195 200 205 tat gee gtc act cgt geg geg gca atg cgt gca tat ett gat aaa gaa

Tyr Ala Val Thr Arg Ala Ala Ala Met Arg Ala Tyr Leu Asp Lys Glu 210 215 220 cag ggc tgg cat aeg tet att tea aat aaa ggc att aat ggc gtg age

Gin Gly Trp His Thr Ser He Ser Asn Lys Gly lie Asn Gly Val Ser 225 230 235 240 •109- 136852-序歹丨J表.doc til 721 1334884 ggt gtc aca caa cca etc tat ttt gac att aac gac age teg act gat 769

Gly Val Thr Gin Pro Leu Tyr Phe Asp He Asn Asd Ser Ser Thr Asp 245 250 255 gtg aac tat etc aat gaa caa ggc ate aeg tgt tgc gtg aat cat aat 817

Val Asn Tyr Leu Asn Glu Gin Gly He Thr Cys Cvs Val Asn His Asn 260 265 270 ggc ttt cgt ttt tgg ggc tta ege aeg act gca gaa gat cca tta ttc 865

Gly Phe Arg Phe Trp Gly Leu Arg Thr Thr Ala Glu Asp Pro Leu Phe 275 280 285 aag ttt gaa gtg tac ace ege act gca caa ate tta aaa gat aeg att 913

Lys Phe Glu Val Tyr Thr Arg Thr Ala Gin He Leu Lys Asp Thr He 290 295 300 gca ggg geg ttt gat tgg gca gtg gat aaa gat att tet gtc aeg eta 961

Ala Gly Ala Phe Asp Trp Ala Val Asp Lys Asp He Ser Val Thr Leu 305 310 315 320

gtg aaa gat att att gaa gca ate aat geg aag tgg cgt gat tac acc 1009

Val Lys Asp lie He Glu Ala He Asn Ala Lys Trp Arg Asp Tyr Thr 325 330 335 aca aaa ggc tac tta att ggc ggt aaa geg tgg ett aat aaa gag ett 1057

Thr Lys Gly Tyr Leu lie Gly Gly Lys Ala Trp Leu Asn Lys Glu Leu 340 345 350 aac agt gca aeg aat tta aaa gat geg aag ttg ttg ate tet tat gat 1105

Asn Ser Ala Thr Asn Leu Lys Asp Ala Lys Leu Leu lie Ser Tyr Asp 355 360 365 tat cac cca gta cca ccg etc gaa cag eta ggc ttt aat cag tac att 1153

Tyr His Pro Val Pro Pro Leu Glu Gin Leu Gly Phe Asn Gin Tyr lie 370 375 380 tet gat gaa tac ett gtt gat ttt tea aat cgt tta gca teg 1195

Ser Asp Glu Tyr Leu Val Asp Phe Ser Asn Arg Leu Ala Ser 385 390 395 taaggggtag aaaatggctt taccacgcaa acttaaattg atgaatttaa teategaegg 1255

taacaaatat ctcggcgaag teaeggaagt gactcaacca aaattagcaa tgaaaatcga 1315 agaatttege gcgggcggta tgattggttc ggtggatgtc aatctcgggc ttgaaaagct 1375 egaageggaa tttaaagccg gtggctacat ggtegaatta attaaaaaat tcggcgggtc 1435 aatcaacggc attccattgc gctttcttgg ctcatatcag cgtgatgaca cagaagaagt 1495 cacatctgtt gagcttgtga tgcaaggtcg atttactgaa attgacagcg gaaacagcaa 1555 agtgggcgat gacactgaac aaacattcaa agtgccttta acgtattaca aaatcattgt 1615 tga 1618 <210> 50 <21X> 398 <212>蛋白質 <213>敗血性巴斯德桿菌

<400> SO 136852·序列表.doc - 110- ί Si 1334884

Gly Asp Leu Cys Leu Lys lie Ser Thr Trp Cys Gin Ser His Arg lie IS 10 15

Asn Gin Ala lie Arg Thr He Gin Ser Leu Ser Thr Ala Val lie Gly 20 25 30 lie Val Cys Thr Ala Asn Asp Ala Asp Asn Glu Thr Phe Pro Leu Asn 35 40 45

Glu Pro Val Leu lie Thr Asn Val Ala Ala Ala lie Gly Lys Ala Gly 50 SS 60

Lys Gin Gly Thr Leu Ser Arg Ala Leu Asp Gly lie Ser Asp Val Val 65 70 75 80

Asn Cys Lys Val lie Val Val Arg Val Gin Glu Ser Ala Gin Glu Asp 85 90 95

Glu Glu Thr Lys Ala Ser Glu Met Asn Thr Ala lie lie Gly Thr lie 100 105 110

Thr Glu Glu Gly Gin Tyr Thr Gly Leu Lys Ala Leu Leu lie Ala Lys 115 120 125

Asn Lys Phe Gly lie Lys Pro Arg lie Leu Cys Val Pro Lys Phe Asp 130 135 140

Thr Lys Glu Val Ala Thr Glu Leu Ala Ser lie Ala Ala Lys Leu Asn 145 150 155 160

Ala Phe Ala Tyr lie Ser Cys Gin Gly Cys Lys Thr Lys Glu Gin Ala 165 170 175

Val Gin Tyr Lys Arg Asn Phe Ser Gin Arg Glu Val Met Leu lie Met 180 185 190

Gly Asp Phe Leu Ser Phe Asn Val Asn Thr Ser Lys Val Glu lie Asp 195 200 205

Tyr Ala Val Thr Arg Ala Ala Ala Met Arg Ala Tyr Leu Asp Lys Glu 210 215 220

Gin Gly Trp His Thr Ser lie Ser Asn Lys Gly lie Asn Gly Val Ser 225 230 235 240

Gly Val Thr Gin Pro Leu Tyr Phe Asp lie Asn Asp Ser Ser Thr Asp 245 250 255

Val Asn Tyr Leu Asn Glu Gin Gly lie Thr Cys Cys Val Asn His Asn 260 265 270

Gly Phe Arg Phe Trp Gly Leu Arg Thr Thr Ala Glu Asp Pro Leu Phe 275 280 285

Lys Phe Glu Val Tyr Thr Arg Thr Ala Gin lie Leu Lys Asp Thr lie 290 295 300

Ala Gly Ala Phe Asp Trp Ala Val Asp Lys Asp lie Ser Val Thr Leu 305 310 315 320

Val Lys Asp lie lie Glu Ala lie Asn Ala Lys Trp Arg Asp Tyr Thr 325 330 335 -111 - 136852-序列表.doc 1334884

Tnr ljys uxy iyr Leu lie Giy uiy Uys Axa Trp Leu Asn Lys Giu Leu 340 345 350

Asn Ser Ala Thr Asn Leu Lys Asp Ala Lys Leu Leu lie Ser Tyr Asp 35S 360 365

Tyr His Pro Val Pro Pro Leu Glu Gin Leu Gly Phe Asn Gin Tyr lie 370 375 380

Ser Asp Glu Tyr Leu Val Asp Phe Ser Asn Arg Leu Ala Ser 385 390 395 <210> 51 <211> 353 <212> DNA <213>敗血住巴斯德桿菌 <220>

<221> CDS <222> (1) . . (351) <220> <223>未知的Cl <400> 51 atg aca tta ttt gat gaa tgt aaa tta get ett aga gac gat ttt aat 48

Met Thr Leu Phe Asp Glu Cys Lys Leu Ala Leu Arg Asp Asp Phe Asn IS 10 15 eta att tgt gat gaa gag aag gat tgt gta aeg gat aag ttt tat ttc 96

Leu lie Cys Asp Glu Glu Lys Asp Cys Val Met Asp Lys Phe Tyr Phe 20 25 30 tat ttc ttg gaa aag aaa gag gaa ttt aat ttt caa gat tat tea ttt 144

Tyr Phe Leu Glu Lys Lys Glu Glu Phe Asn Phe Gin Asp Tyr Ser Phe 35 40 45 gaa gaa atg tat ata ttt tea aaa atg gaa cct gtg tat gtt tta tgt 192

Glu Glu Met Tyr lie Phe Ser Lys Met Glu Pro Val Tyr Val Leu Cys 50 55 60 gat age tet aat ata cct ttg ttt agg agt aat tgg gaa ttg att ate 240

Asp Ser Ser Asn He Pro Leu Phe Arg Ser Asn Trp Glu Leu He He 65 70 75 80 aat aat ata tat gat gtt gtc tgt tta tet aca aaa gta ttt ttt eta 288

Asn Asn He Tyr Asp Val Val Cys Leu Ser Thr Lys Val Phe Phe Leu 85 90 95 gat gat gaa aag tta atg atg gaa tta ttt ccz gaa gat aaa gta aga 336

Asp Asp Glu Lys Leu Met Met Glu Leu Phe Pro Glu Asp Lys Val Arg 100 105 110 gtc ate tat aaa aga ta 353

Val lie Tyr Lys Arg 115 <210> 52 <211> 117 <212>蛋白質 <213>敗血性巴斯德样菌 -112· 136852-序歹!1表.doc i si <400> 52

Met Thr Leu Phe Asp Glu Cvs Lvs Leu Ala Leu ArQ Asp Asp Phe Asn 1 5 10 15

Leu He Cys Asp Glu Glu Lys Asp Cys Val Met Asp Lys Phe Tyr Phe 20 25 30

Tyr Phe Leu Glu Lys Lys Glu Glu Phe Asn Phe Gin Asp Tyr Ser Phe 35 40 45 31u Glu Met Tyr lie Phe Ser Lys Met Glu iro Val Tyr Val Leu Cys 50 55 60

Asp Ser Ser Asn lie Pro Leu Phe Arg Ser Asn Trp Glu Leu lie lie 65 70 75 80

Asn Asn lie Tyr Asp Val Val Cys Leu Ser Thr Lys Val Phe Phe Leu 85 90 95

Asp Asp Glu Lys Leu Met Met Glu Leu Phe Pro Glu Asp Lys Val Arg 100 105 110

Val lie Tyr Lys Arg 115 <210> 53 <211> 509 <212> DNA <213>敗血性巴斯德捍菌 la <220> <221> CDS <222> (1)..(507) <220> <223>未知的C2 <400> 53 atg aaa aat ttt agg aat ata aat att tat agt gat tat gga aag gtt Met Lys Asn Phe Arg Asn He Asn lie Tyr Ser Asp Tyr Gly Lys Val 15 10 15 gat aag gaa att ata tta gaa ttc gaa aat gaa ttt aat ata aag ctt Asp Lys Glu lie lie Leu Glu Phe Glu Asn Glu Phe Asn lie Lys Leu 20 25 30 cct tct tta tac ata gat tta att acg gcg cat aat get ccg aag agt Pro Ser Leu Tyr lie Asp Leu lie Thr Ala His Asn Ala Pro Lys Ser 35 40 45 gaa gag aat tgc ttt gaa tat tac aat gag cgt aat gag ccc acg ttt Glu Glu Asn Cys Phe Glu Tyx Tyr Asn Glu Arg Asn Glu Pro Thr Phe 50 55 60 tct tcc ttt gga ttt gaa ggg ttt gag aca gag egg tct age gcc tct Ser Ser Phe Gly Phe Glu Gly Phe Glu Thr Glu Arg Ser Ser Ala Ser 6*E 70 75 80 ctt gaa aat ata tat get cag tat att tat gat gat cca ate tat ggt Leu Glu Asn lie Tyr Ala Gin Tyr lie Tyr Asp Asp Pro lie Tyr Gly 85 90 95 48 96 144 192 240 113- 136852-序列表.doc

I 288 1334884 <210> 54 <211> 169 <212>蛋白質 <213>敗血性巴斯德桿菌 <400> 54

Met Lys Asn Phe Arg Asn lie Asn lie Tyr Ser Asp Tyr Gly Lys Val 15 10 15

Asp Lys Glu lie lie Leu Glu Phe Glu Asn Glu Phe Asn lie Lys Leu 20 25 30

Pro Ser Leu Tyr He Asp Leu lie Thr Ala His Asn Ala Pro Lys Ser 35 40 45

Glu Glu Asn Cys Phe Glu Tyr Tyr Asn Glu Arg Asn Glu Pro Thr Phe 50 55 60

Ser Ser Phe Gly Phe Glu Gly Phe Glu Thr Glu Arg Ser Ser Ala Ser 65 70 75 Θ0

taC gaa cat gtg tat tet ttt ggt agt act 99« gag gga cat ttt ate 336 Tyr Glu His Val Tyr Ser Phe Gly Ser Thr Gly Glu Gly His Phe lie 100 105 110 tgt ttt gat tat cgt gat gat cca aaa ggt gat gaa ccc aaa ate tgt 384 Cys Phe Asp Tyr Arg Asp Asp Pro hys Gly Asp Glu Pro Lys lie Cys 115 120 125 ate gtg att cac gat gaa cat gat gaa aaa aca ggg aaa atg ega ctg 432 lie Val lie His Asp Glu Tyr Asp Glu Lys Thr Gly Lys Met hrg Leu 130 135 140 ttt cct ata gca gag aat ttt gaa geg ttt tta gat agt ttg aaa tea 480 Phe Pro lie Ala Glu Asn Phe Glu Ala Phe Leu Asp Ser Leu Lys Ser 145 ISO 155 160 ttt gat gaa atg ata gag aag tat teg ta 509 Phe Asp Glu Met lie Glu Lys Tyr Ser 165

Leu Glu Asn He Tyr Ala Gin Tyr lie Tyr Asp Asp Pro lie Tyr Gly 85 90 95

Tyr Glu His Val Tyr Ser Phe Gly Ser Thr Gly Glu Gly His Phe He 100 105 110

Cys Phe Asp Tyr Arg Asp Asp Pro Lys Gly Asp Glu Pro Lys He Cys 115 120 125 lie Val lie His Asp Glu Tyr Asp Glu Lys Thr Gly Lys Met Arg Leu 130 135 140

Phe Pro lie Ala Glu Asn Phe Glu Ala Phe Leu Asp Ser Leu Lys Ser 145 150 155 160

Phe Asp Glu Met lie Glu Lys Tyr Ser 165 <210> 55 c211> 443 -114- 136852·序列(表.doc

l SI 1334884

<212> DNA <213>敗血性巴斯德桿菌 <220> <221> CDS <222> <1)..(441) <220> <223>未知的C3 <400> 55 atg ata aaa tat tta gag gga aat att aac teg ttt ata teg gca tta 46 Met 1 lie Lys Tyr Leu 5 Glu Gly Asn lie Asn 10 Ser Phe lie Ser Ala 15 Leu ggt aaa aac gaa agt aat aaa gat ate tta aaa Cta gta gaa ata gtt 96 Gly Lys Asn Glu 20 Ser Asn Lys Asp lie 25 Leu Lys Leu Val Glu 30 lie Val tct tea gat ttt gaa gtg gat gaa eta agt cat aaa gat gaa cac gag 144 Ser Ser Asp 35 Phe Glu Val Asp Glu 40 Leu Ser His Lys Asp 45 Glu His Glu ata tat tat ttg ttt tat aag agg ggt gtt gaa ttt tgt ttt aaa aga 192 lie Tyr 50 Tyr Leu Phe Tyr Lys 55 Arg Gly Val Glu Phe 60 Cys Phe Lys Arg ata gat gaa gag tat gtc tta tat teg gtt ttc ttt ttc ttg gta gag 240 lie 65 Asp Glu Glu Tyr Val 70 Leu Tyr Ser Val Phe 75 Phe Phe Leu Val Glu 80 gtt gat aat tat ttt tea tgc cca ttt att cat gaa tta ata tgt gat 288 Val Asp Asn Tyr Phe 85 Ser Cys Pro Phe He 90 His Glu Leu lie Cys 95 Asp ctt aaa cac gga ttc tea ata gag gat att ata agg ttt tta ggg gag 336 Leu Lys His Gly 100 Phe Ser lie Glu Asp 105 lie lie Arg Phe Leu 110 Gly Glu cca aat ttt aaa ggt agt ggc tgg gta aga tat tct tat aat gga aga 384 Pro Asn Phe 115 Lys Gly Ser Gly Trp 120 Val Arg Tyr Ser Tyr Asn Gly Arg 125 aat att cat ttc gaa ttt aat gaa tct aat gaa tta tcc cag att age 432 Asn att lie 145 lie 130 ttt Phe His att lie Phe ta Glu Phe Asn 135 Glu Ser Asn Glu Leu 140 Ser Gin lie Ser 443 <210> 56 <211> 147 <212>蛋白質 <213>敗血性巴斯德样菌 <400> 56

Met lie Lys Tyr Leu Glu Gly Asn lie Asn Ser Phe lie Ser Ala Leu 1 5 10 15

Gly Lys Asn Glu Ser Asn Lys Asp lie Leu Lys Leu Val Glu He Val 20 25 30 136852-序歹 Ij 表.doc - 115· i si 1334884

Ser Ser Asp Phe Giu \^ai Asp GXu Leu Ser His Lys Asp Glu His 61u 35 40 45 lie Tyr Tyr Leu Phe Tyr Lys Arg Giy Val Glu Phe Cys Phe L»ys Arg 50 S5 60

He Asp Glu Glu Tyr Val Leu Tyr Ser Val Phe Phe Phe Leu Val Glu 65 70 75 80

Val Asp Asn Tyr Phe Ser Cys Pro Phe lie His Glu Leu lie Cys Asp 65 90 95

Leu Lys His Gly Phe Ser lie Glu Asp lie lie Arg Phe Leu Gly Glu 100 105 110

Pro Asn Phe Lys Gly Ser Gly Trp Val Arg Tyr Ser Tyr Asn Gly Arg 115 120 125

Asn lie His Phe Glu Phe Asn Glu Ser Asn Glu Leu Ser Gin He Ser 130 135 140

He Phe lie 145 <210> 57 <211> 6496 <212> DNA <213>敗血性巴斯德样菌 <220> <223>未知的C <400> 57 gaattcgaat taagcgagaa aattgctgaa acactagaac aaagtcaatt aaatattagt 60 caattatcaa ttgttgaaat ttatcctttc aatgaagaac aagggatacg ttttcataat 120

ttatcttttc 180 gggttgcgtg 240 gataccggga 300 agccgatcca 360 aatcaaagac 420 aggtaaatta 480 acaacgttta 540 caagatcttt 600 cgggatgggg 660 agcgagctgg 720 gacgaatggc 780 aagtgcggtg 840 tacttcatta 900 aaaagtgtgg tttgctggcg gtgattgata gtaaatcagg caagtgacac attgccgtaa gcgggacaaa gcttttgatg ccacaattag caaatggtga accgacaatc gaacaggaaa gaaaatggtc tacaacttaa atattcagca tgaaagggat aaaaattggt aacttgcatt cctcgttatt cagcgcgatt ttttccctac ataatgtcgt gcgtattatc cgttgatgac tggcagaaga tacaattttg accagaagag agtcgctcat ttgtgccagc agatttacgt agtcatcgcc acctgcatct gttaaatggc gcctgcatcg atttcattct ggattatgca ttatcatgca gcaagcagca gtcggtcgca gtggaatggt ctcgcgaaag ttgcaagacg cagcgtaata tcgttgatgt tatactaacg attccacttg catttaaata atccaagtgc ttagatcctc gaaaaatatt aaattacata gatgaacagc catcaatcca ccgcagaaat tccctgtggt ttgtgtcctt caaatcacga gagaaacggt atgaaggcga tgcaaattca ctgttttcta aatcttgctt gcaccccagt taagtgggtt gctttaatct -116- 136852-序列表.doc 132 1334884 agtgttacgc ttgcagagtt aatttactcg caaggttatt aatctaaatg tgtttttgca 960 cgatattttt atcttgaact ttgagagcgc actcgttttt gacgagtgcg tttttgttaa 1020 aacattcgtt tgaaagacag tgaatgaata gcggagttat tgataagaat caatttatac 10Θ0 aaaagcaact gaatgttatt aatcgaggca ataaacctat tgatagtttt agttggcgcc 1140 ataatacata aactgtactt aataatatgc aatcaatacc tagaaatatt catgacgtaa 1200 tccaacatat cggggagggg attttaagtg atggtagaaa aaacatttag aaatctaaaa 1260 atatatgatg attatggttc tgtctctcag gaaattattt ttaattttga aaaagagttt 1320 gatataaaac tccctttatc ctatatctca cttgtgaaaa agtataatgg cgtttggttt 1380 aaggaaagtg attttgaata tttatctcaa aatgggaaaa gaataataag ctcattgagt 1440 tttgatagtt ttgagacaaa agataatatc gaaccaatga ataatatatt aagacaatat 1500 atttatgatg atgaaattta tggatataag aatgtttatt cctttggtta cactggaaat 1560

ggtgactttg tctgttttga ttatcgtgat gacccaaaag gtgatgagcc caaaatctgt 1620 atcgtgattc atgatgaata tgatgaaaaa acaggcaagc gtttgttatt gcctgtggca 1680 gaaaattttg aggcattttt agatatgctt tacgattttg atgaacgcta tccgaatggt 1740 tatgaatagg tatttgttta aataatgtgt tgtatttttt aagcattatt tacaactaac 1800 attttaagtg cggtcaattt tgaaaaagtt ttgggctttg agaattgggc gcattttttt 1860

tgaaatattc tggatggtaa tttttgactt tagtactgaa ttatttgatg aaggattgtg tttcaagatt ttatgtgata atatatgatg atggaattat cgcgctcgtg agtgaggttg gataaaatag ttaataccta ggaaagtgat tgataaggaa ttcaatgatg taaaggatgg tactaaaggt caagagtttg aatgtaaatt taatggataa attcatttga gctctaatat ttgtctgttt ttcctgaaga tcagcacgag atcgtaatca agggatttac caccaattca aaaatgaaaa attatattaq agcactaatt aggctagatt aaaggactag aaaaatttta agctcttaga gttttatttc agaaatgtat acctttgttt atctacaaaa taaagtaaga ttcgctttca atttactgaa atggcatcat taaggctgtt attttaggaa aattcqaaaa atggattaga ttgatcctga gtaaagcagt aagcaattaa gacgatttta tatttcttgg atattttcaa aggagtaatt gtattttttc gtcatctata ttaaagctct gaacagttaa aactcacaaa cagcatatag tataaatatt tqaatttaat taatgggaat gaaagttgtt taaaaagtca ataaggaaga atctaatttg aaaagaaaga aaatggaacc gggaattgat tagatgatga aaagataatt cgttaaagac ttaaaattaa gtagtcccca gtgaaggcgc tatagtgatt ataaagcttc tatcgagata 1920 catgtaaata I960 ttctttttga 2040 taaaatgaca 2100 tgatgaagag 2160 ggaatttaat 2220 tgtgtatgtt 2280 tatcaataat 2340 aaagttaatg 2400 accccttgat 2460 tagcactagc 2520 taagaggctt 2580 aaatatgtag 2640 tttaagtgaa 2700 atggaaaggt 2760 •117- 136852·序列表.doc 1334884 catagattta attacggcgc ataatgctcc gaagagtgaa caatgagcgt aatgagccca cgttttcttc ctttggattt gtctagcgcc tctcttgaaa atatatatgc tcagtatatt ttatgaacat gtgtattctt ttggtagtac tggcgaggga tcgtgatgat ccaaaaggtg atgaacccaa aatctgtatc tgaaaaaaca gggaaaatgc gactgtttcc tatagcagag tagtttgaaa tcatttgatg aaatgataga gaagtattcg catagcgatg ctctctcccc tgattttatg gaaattttgt aatgaaaaaa ttttatttaa tttttttatt attcctaagt atgtatctat actcctcaga tggtaaattg ttttgttgat aatatctcgg tatcaaaagc cttatagtct aggaaaaacc ggatgttatg ttttgtggag gtaaatatgg tgattataaa ggaacaatcg gataaattac atcattgtat gacccaaaaa cgcagaatgt ggataccaaa atcctaaatg ggataaaggc ttggatttta ctttacgaat tttttttgaa aaaagttacc agcccaggtg gcgcttgttg gatgtgttat tcgcatagta aatgaattca ttattaaaaa tggaattaaa gagcgtaatt atttaatttc tcctgatgtt atggaaatat tatttaagtt aagttgttat tgttcagttt attattactt ttaaatggat gagtgtttta gaccgttaat tcaggtgggg tcatctgctt atatttccat caatagcacg tttccaaaaa gtagagaaca caacgttgga aagatgcggt tgattgtgga agtaagtatg ataaatgata ataatttata tagcattttt cactcctgta aagtttcatc ccgcagaatg tggataccaa aatcctaaat ttataagtgt tttgaatttt actttacaaa ctttttttga tctgttttta gggataaaga ttcgttcatt ttctttaaat cttgattatc aaataataat atcgtgttcg tgtccacacg ctcctcaaaa gcgagtgcta gctgtggggt aaacggtggg aaaccaattg accgcataac gtgcggtcaa tttctaagat gaccgaaaag tgcggtaaag gggtaacgaa acacaaaaaa gtgtcattaa accccagaaa aaaacttgac cgattgttgc gtaagccgac ttcataqggt aqcattqcaa taaaccagat 136852-序列表.doc -118- gagaattgct ttgaatatta 2680 gaagggtttg agacagagcg 2940 tatgatgatc caatctatgg 3000 cattttatct gttttgatta 3060 gtgattcacg atgaatatga 3120 aattttgaag cgtttttaga 3180 taatgtctcc gttcatatct 3240 ttaaattagg agggcactaa 3300 ggatgttttt atcatgatgg 3360 aaaggagata tatttccatc 3420 aattcagaac agcgttggaa 3480 ttagagaata taaaaacagt 3540 gggtatatcc atttaactcc 3600 gtttgtaatt tataagtgtt 3660 gcaggtatgt ttttaatcaa 3720 gttattatgc ggaagtgcta 3780 agcaccgttt gcggtgagtg 3840 aggaggtgat taggatgaaa 3900 gcctatattc ttttgaagag 3960 gtcataagaa taaaggagat 4020 tcggaaaaac tgatgccaag 4080 gcgatgaaga tttaatatat 4140 tggttaaaaa aggatataaa 4200 gggataaggg tatttgtaat 4260 aaaatgttac cgctggtatg 4320 ttaggcacgg tataaaagag 4380 agtagggttt gattaaagta 4440 aaagaaagta aagagtatat 4500 ttatgagttc cagaaaaata 4560 taaaaatatg acagcaagct 4620 actgctgaaa atggcaaaaa 46Θ0 taatccactt gaatoatcac 4740 IS] 1334884 ttttcattac ggactcctta tttatggctg ataacttaac acttgtattg aaaattaaag 4800 cagatttaaa caatgcgttg agcgatttta aagcgcttaa aacggaattg caacgcgcag 4860 gcgtggagtc tatcagtaag taactcgcca gtttttgcaa tcatttatca tattcacaaa 4920 gccgtaatgc atattggaga aggttaagtg taagtgaagg aagataaaat gaaatttaga 4980 cattttggca tttattatga ttatggcgta gtttcccaag agattatttt agattttgag 5040 gaaacgtttg gtattaaatt accaa^attg tatattgagt tgatcacaaa acataattcc 5100 ccaagattga atagagatca tttcaattat tatgatttct ctacgagtga ggaagaagga 5X60 acggaattta tattcaaagg atttgaaaca gaaagtaata ggcatgcgcc gccagaaaat 5220 atttatgcac aatatcttta tgatgatgaa atatatggtt atgaacatgt gtattctttt 5280 ggcagtaccg ctagtggtga ctttgtctgt tttgattacc gagataatcc gaaaggcagt 5340 gaacctaaaa tttgtcttgt gattcatgat gaatatgatg aagaaacagg caagcattta 5400

ttgtttcctg tggcagaaaa ttttgaggta tttttagata tgctttacga ttttgatgaa 5460

cgctatccga atggttatga ataggtattt aaataatgta ttgtattttt taagcgttat 5520 ttaataacgt tttaagtgcg gtcaatttca aaaaagtttt gctttgagga attgaccgtt 5580 tttgtatcat ggatatgatg tgagcaattg atatcctgtt tgtgttaact ggcatgtggt 5640 aaagcattcg tcaaagacga gctctagtgg tgagggacta atcatgaatg aactattgga 5700 cttttataga aatataactt tttttgaaga atatgatgaa aactcattca taggaagatg 5760 gcttgattac tcggaatgga atgataaaga atattggaaa ttagagaaag atttactgaa 5820 aattgctcaa atgtatagaa ctactaatga agtttttcca gatattttaa taggggttat 5860 gcgtatcatt gaattattaa tgatacctaa ctggaatagt tttataatat ctaattcaga 5940 aagtgttgat atttatgata gatatgaacg atttaaatat ataatttcaa tacttttcaa 6000 taataaagat gttagactag atggcttttc atatacagaa aagaatgatg atgtatttta 6060 taatgaatag aaaaattaaa agtaagctaa tttgtagaaa taataaaagt ctcgcttgta 6120 ttttttcttt gagatcgtgc ttgagtccac acgagtgcta gctgtaggca gagtgtcttg 6180 gtgattataa aaagttaagg atagtattgt tgatatgaaa gaaaagacta aatattcaat 6240 ggggaatcaa agtgataaaa aataaatttt atatttcttg gttaggattt tcaatatcat 6300 tatatgtttt gcttttatgt agagatttgt tttattctct ttatatgttt tttattatga 6360 tgtatttgtc ttaaaatttt cttttaagga ggcgtttttt actatcttta tttttattgt 6420 gctatgttcc attattcatt attcattatt cattattcat tattcattat tcattatttt 6480 atatataaat atggtaaaag gtctgaatgc ccaaaaaaga aaccattaag tgaaaatgta 6S40 acatacaata aaaggtagta ggaataaata aaatattagg aataacagaa tgataaaata 6600 tttagaggga aatattaact cgtttatatc gqcattaqqt aaaaacqaaa qtaataaaaa 6660 •119- 136852-序列表.doc I S3 1334884 tattttaaaa ttagtagaaa tagtttcttc agattttgaa gtggatgaac taagtcataa 6720 agatgaacac gagatatatt atttgtttta taagaggggt gttgaatttt gttttaaaag 6780 aatagatgaa gagtatgtct tatattcggt tttctttttc ttggtagagg ttgataatta 6840 tttttcatgc ccatttattc atgaattaat atgtgatctt aaacacggat tctcaataga 6900 ggatattata aggtttttag gggagccaaa ttttaaaggt agtggctggg taagatattc 6960 ttataatgga agaaatattc atttcgaatt .aatgaatct aatgaattat cccagattag 7020 catttttatt taataattta ggattgggaa cgctctcgtg ttcacacaag tgcggtttga 7080 ttaaagcact tgtcaaaggc gagatccagc tgtgggagat ttgatagaga aatggctctc 7140 aggattgacg ttagtagagg gagcataaat caagaaaaaa catttaaaat aggaacatta 7200 ggtggtgagt aatggaaatc actcgtttta aaatcctact tattgccttg attatattag 7260 gtaatgggta tgtgtttctt ggtggaccag ctaaaaaaca attcgctata gaagcagaaa 7320 caaggcggat ttatcgtact ttaacgaaag aaattatttt tatgaatgga gaatataaac 7380 aatttggagg gcgagttatt caaggattta ctttagtcat ttctttttct cattcaagta 7440 cagaaaaccg cattaagatt ttagaaaaaa ttaaagagat gggatttgat tcaaaaaata 7500 aaacatcaaa accgagtctt catctttttt gtcaaggaga aagtggtttt ttgatcgcag 7560 aaaaacctga atttagaata gattatgaga aaaagatgac ttattgtttg gagtagcaaa 7620 ggaaggcaat gataagtgta ttttctaatc agatttaatt taagtattaa agtgcggtca 7680 attttgaaaa cgttttactt tgaaaaattg accgtttttt tacatgagag agtgaaagta 7740 caccacctta ggcaatgtgg ttgtggataa ggcgattaag gaatacccaa atgggggtta 7800 tgaagcgagg gtgctgatcc ctaacccgaa agcgaaaacc gatcctgatg cgccgaaatt 7860 tttggagaaa aggggaaatg aaggtgtatc cacaatgttt ccaagaacat ggacagagga 7920 taggttgaaa gtggagttag agcatgcgtt taataataga gtaccaatgg aaaagtttga 7960 aaataaatgg gaaggtataa caaaatcagg cgtgaaagta gaatgggttc tggatagaaa 8040 tggtaaagtg ttgactctat tttgttaatt ttctgtgtct ttttgtaaaa aataagttgt 8100 tgaaataaca tttttctcta taatcaaatc ttaatgataa ttaatatcat taaataagaa 8160 tggatactta gatgaggatc atatgaaaga tttatcaaaa atgacctgcc ttgaagactt 8220 acgccgtgtt gcgcaacgta aagtgccaaa aatgttttat gactatgtag tttcaggatc 8280 ttggagcgaa agcacattgc atgccaaccg taatgatttt caagcaatta agctacgtcg 8340 acctgtggcg attttgccca tttctgcatt cagctctgca attcgtcgct tgtgctgttc 8400 tgttgcgcgc gcaagccctc ttgtcgactt gccttttcta attgggcttg ttgctcccaa 8460 taacgtgagg tcgccacatc cactttacgt agtgttga 8498 -120- 136852·序列表.doc IS! 1334884 <210> 58 <211> 5798 <212> ΟΝΛ <213>牧血性巴期揀桿菌 <220>

<221> CDS <222> (2686) . . (4446) <220> <22，>未知的m

<400> 56 gtcgacaaga ccttttttaa ttattattat ggtttatcta ttgaaagcta gtaaatgttc aagaaactta acgggattat atgttttgta attcagttgt acattatcag ttaattctag aatcgttttt catgacacag tacgcaaaat tccttcaata tttactttat attacagata tgaactctta tgcgcaaaat tattaatgat taataatgct gattcataat cattgttaaa cacacatgta ataaaacgaa agattctagc caggcctata ttattttagt aggtgaaaaa aaacagggat atccagatct cacaagcgtt ctgttttaca ctgcagatat ctaaaatgag caccaggtat gttagtttag aaaagtatgt tattcggcac cgctataaat ttaacgccat aaaaatctaa ttaaaatcac gggaatttgg aatgttgcta gtttctttac cttgtttttg gagcgtttaa tcaaaaggtg acagcaaata tgttttagat ttatttatat ttttattatt aatcatggta tagtattgat tacttatgtg acaggatctt acagagttta cagggagata tatcccattg aatgagggta ctttatctgt catctgaaac tcatttcact tagcgaagac tactgaatga ttcaattaaa ctaatgaagg tgaatgcccg ttaataattt tgttgccaca ctctagttaa atacttcacc taactcggta gaattagaat aaaaatatac gcagtaaaaa gtcattccag gatttaccag gcggctttaa atgttaagga tcgatctcat aattttggtt caattattaa attttgttta tttccaaatg cgttggttgt aataacggca gtatcgcgat atactatcag taatacgaag taatttccaa agaagtatat aaatgatatc agccatgcta atatttaaat aaatggtgat agatgattta acactdagga tactacttat gtgtgaagct acgattcaaa ggattttgac gttttattga aacaagtggc ttattagaaa tgccaacaac cttcaattta tcattgagga ttatgttccc ttttttaaat tctacccatt aaagagactt gtgttgaaag cgaggtgaca cttgctacac gaagcaattt aatttaagag aataaagcaa aatattatta ggcgttaaga cttgattatg attaatgcat tttgatatga taacaataat 60 tttggtcaca 120 ttttgtcata 180 aaatgctatt 240 cttagttgca 300 aatcgatttt 360 atctgaactt 420 agaaataaga 480 ttctcactta 540 aaagttaggt €00 aataatcatt 660 ttaccaagaa 720 ctcagcaagg 780 tatatgaaag 840 ccaaagaaga 900 gcaaatcttc 960 aagcgaaaat 1020 ctgtcgcaaa 1080 gtatcgctta 1140 gagagttctt 1200 atggcgattt 1260 attctcgatt 1320 caaaagatat 1380 tgaaaaaaac 1440 -121 - 136852·序列表.doc 1334884

atcaaatata aaaggaacct cgtcaattga atttgctttg acgatagcgt tctatttatt 1560 tgttgtgatg tttatttttg aattttgtcg attagcggtt gcgacagctt attgggattt 1620 agctataacg gaaagtgtca gaattgcgaa gaatgaacaa gcaatttctg gaaattatga 1680 agaagcattt aggaaagctc ttacaaagca aaaaaaattc catgatgaat cgacaattgg 1740 atatttggcg ttgttagaag ataataaatt tgatgtaaaa gtccaatatg tggattgtga 1800 taaagaaacg gaatgtatta aaaatcttct gcttaataaa tttcgccaac cacaaaaaaa I860 tcataaagga gagttaatct ctcctacggg gagtcgcgcg actttagcac aatattcttt 1920 aacttataaa tataagttta tggtgccgtt agtatttatt cctgagtctt ggtctcaagt 1980 agtgctgaac cgtgaatttg ttgttgtaca ggaatttgag cgttctcaat ttatgttagg 2040 agcaaaacca agttctcttg ggacgaatcc atagaaaatt tactcattat ttcgagctat 2100 atatgaaaga gtcaggttta gttaaattca agcatttttg gaaaaataaa aagggcgcag 2160 tgacgataga gttccttttt atgtcaatgt ttctgattgt gctatttgca tttctcttcg 2220 atttagtaat gttacgttct acattaggca agttagataa tgcctcatat acattagtta 2280 gtattctccg tgaacgtaca cagttgtacg atagagttgc acaaattaat attgatgatc 2340 ataagcaatt tgaaaagctt gctaagaaac tgatttatgg tgatcagaat agtaataaaa 2400 ggatcgatgt tgttttagaa tattgggcac aagacggttc tggacggagg attccaaata 2460 tcattggcga ttgtacgcct tacaaaaaac tttctgattt atcctattta tctcctcgct 2520 cagaactcaa taatgaaaga aaaataccgc tttatcaaat tactctttgt gttgaaactc 2580 agggcttgtt tgaaacaata ttactggata agtctgagcg ttcaacgggg ctgattagat 2640 catcgtcaat gtcagtatca cgataaatta tcgttaggga acttt atg aaa aaa ctt 2697

Met Lys Lys Leu 1 tat tta att cgt tct tgc tat gat tea gtc aga aaa ttt tat gag aat 2745

Tyr Leu He Arg Ser Cys Tyr Asp Ser Val Arg Lys Phe Tyr Glu Asn 5 10 15 20 gag eta ggt gtt tat aca gta atg act gca tta eta gca ttt cca tta 2793

Glu Leu Gly Val Tyr Thr Val Met Thr Ala Leu Leu Ala Phe Pro Leu 25 30 35 tta gtt ttg att gga ttt aeg gtt gat gga act ggg gtt gtg ctt gat 2841

Leu Val Leu He Gly Phe Thr Val Asp Gly Thr Gly Val Val Leu Asp 40 45 50 aaa gca cgt tta get caa gga atg gat caa get get tea get ttg gtt 2889

Lys Ala Arg Leu Ala Gin Gly Met Asp Gin Ala Ala Leu Ala Leu Val 55 60 65 get gaa aac aat gac tac ega gaa aat aaa aaa cat ggt gat gtt aat 2937

Ala Glu Asn Asn Asp Tyr Arg Glu Asn Lys Lys His Gly Asp Val Asn 70 75 80 cqq caa qca qta teq cct caa qae aaa gca aaa ttt aat qat aat aaa 2985 • 122· 136852·序列表.doe 1 Si

Arg 85 Gin Val Val Ser Pro 90 Gin Asp Lys Ala Lys 95 Phe Gly Gly Asn Glu 100 ttt atg geg aaa caa gaa aag cgt aat caa gag ctt ate cag ggt att 3033 Phe Met Ala Lys Gin 105 Glu Lys Arg Asn Gin 110 Glu Leu lie Gin Gly 115 He gcc aaa ctt. tat r.ta cgt tea gaa aat geg aat get tea tet gat gca 3081 Ala Lys Leu Tyr 120 Leu Arg Ser Glu Asn 125 Ala Asn Ala Ser Ser 130 Asp Ala cca ate act att gat aaa cct ttt cat tat tea tgt gag gag tta gat 3129 Pro lie Thr 135 lie Asp Lys Pro Phe 140 His Tyr Ser Cys Glu 145 Glu Leu Asp tta cct aca get aat gag tat gca cgt cgt aaa cct att gtt tgt gaa 3177 Leu Pro 150 Thr Ala Asn Glu Tyr 155 Ala Arg Arg Lys Pro 160 lie Val Cys Glu gtg caa ggt ggc gtc aat cgt aaa ttt tgg ctt cct gtc agt gaa teg 3225 Val 165 Gin Gly Gly Val Asn Arg Lys 170 Phe Trp Leu 175 Pro Val Ser Glu Ser 180 tta gtt agt gaa gat aaa ctg aaa aaa gat ega gtt aga ctg gaa tcc 3273 Leu Val Ser Glu Asp 185 Lys Leu Lys Lys Asp 190 Arg Val Arg Leu Glu 195 Ser gat acc agt tat geg att aaa gaa aaa ggc ate gtg att cct gtg gag 3321 Asp Thr Ser Tyr Ala 200 lie Lys Glu Lys 205 Gly lie Val lie Pro 210 Val Glu eta atg ctt gtt teg gat tat tet ggt teg atg aat agt cat tta cag 3369 Leu Met Leu 215 Val Ser Asp Tyr Ser Gly 220 Ser Met Asn Ser 225 His Leu Gin gat aaa aac ggt aga tet eta gga aaa get aaa att act att tta aga 3417 Asp Lys 230 Asn Gly Arg Ser Leu Gly 235 Lys Ala Lys lie 240 Thr lie Leu Arg gaa gtg gtt agt gaa att teg aaa att tta ttg cca gaa gat gtt age 3465 Glu 245 Val Val Ser Glu lie 250 Ser Lys lie Leu Leu 255 Pro Glu Asp Val Ser 260 gaa ggt gtg age cct ttc aac cgt att ggc ttt aeg act ttt tet ggc 3513 Glu Gly Val Ser Pro 265 Phe Asn Arg lie Gly 270 Phe Thr Thr Phe Ser Gly 275 ggt gtt aga caa egg gat gtc act gaa ggc tgt gtg ctt cca tat gaa 3561 Gly Val Arg Gin Arg 280 Asp Val Thr Glu 265 Gly Cys Val Leu Pro 290 Tyr Glu gga aaa ata tea caa act tet ega aaa tta act att cgt tat tgg att 3609 Gly Lys lie 295 Ser Gin Thr Ser Arg 300 Lys Leu Thr lie Arg 305 Tyr Trp lie aeg ggt aat aat aca cct tgg aaa ttt aat get 999 aga tgg gag aga 3657 Thr Gly 310 Asn Asn Thr Pro Trp 315 Lys Phe Asn Ala Gly 320 Arg Trp Glu Arg age aca gtg tet ttc cag gag cat tat aaa ggc tat tat gac aaa ttc 3705 Ser 325 Thr Val Ser Phe Gin 330 Glu His Tyr Lys Gly Tyr 335 Tyr Asp Lys Phe 340 136852·序歹！1 表.doc -123- (Si 3753 cat tec tea act tgt aga ggc tea ggg age tet aga act tgt caa att His Ser Ser Thr Cys Arq nly Ser Gly Ser Ser Arq Thr Cys Gin lie 345 350 355 gat gca aat cct aag aaa att atg gat tat gca eta aaa att aat gac 3801 Asp Ala Asn Pro Lys Lys lie Met Asp Tyr Ala Leu Lys lie Asn Asp 360 365 370 tgg aeg aca att aga gaa tta ttt aat act tat ata gat gta agt ggg 3849 Trp Thr Thr lie Arg Glu Leu Phe Asn Thr Tyr lie Asp Val Ser Gly 375 380 385 aeg att gac caa att tee cag ttt gat ggt tea aac aga cgt tat gat 3897 Thr lie Asp Gin lie Ser Gin Phe Asp Gly Ser Asn Arg Arg Tyr Asp 390 395 400 atg gtg ttt act gat gaa gaa egg tgt ett ggc gga aat att ggt aga 3945 Met Val Phe Thr Asp Glu Glu Arg Cys Leu Gly Gly Asn lie Gly Arg 405 410 415 420 aga aca act ega get tgg ttt gat caa aaa aat aaa gat att aca aga 3993 Arg Thr Thr Arg Ala Trp Phe Asp Gin Lys Asn Lys Asp lie Thr Arg 425 430 435 gag ttg aat att gtt cgt cct tet ggt tgg act tet gca tet teg ggg 4041 Glu Leu Asn lie Val Arg Pro Ser Gly Trp Thr Ser Ala Ser Ser Gly 440 445 450 ett ett gtt gga get aat ate atg atg gac gag aat aag aat cct gat 4089 Leu Leu Val Gly Ala Asn lie Met Met Asp Glu Asn Lys Asn Pro Asp 455 460 465 geg caa cct teg aaa etc ggg aca aat att caa cgt gtt ate tta gta 4137 Ala Gin Pro Ser Lys Leu Gly Thr Asn He Gin Arg Val lie Leu Val 470 475 480 tta tet gat ggt gaa gat aac tgg cca act tat agt aca tta aeg act 4185 Leu Ser Asp Gly Glu Asp Asn Trp Pro Thr Tyr Ser Thr Leu Thr Thr 485 490 495 500 ett tta aac aat ggt atg tgt gac aaa att ega gaa caa ttg ggc aag 4233 Leu Leu Asn Asn Gly Met Cys Asp Lys He Arg Glu Gin Leu Gly Lys 505 510 515 tta caa gat cca aat tta ega gag tta cca gga aga att geg ttt gtt 42Θ1 Leu Gin Asp Pro Asn Leu Arg Glu Leu Pro Gly Arg lie Ala Phe Val 520 525 530 geg ttt ggc tat agt cca cca gca aac caa gtt gee get tgg aaa aaa 4329 Ala Phe Gly Tyr Ser Pro Pro Ala Asn Gin Val Ala Ala Trp Lys Lys 535 540 545 tgt gta ggt gac caa tat tat aeg get tat teg aaa gaa gag ttg tta 4377 Cys Val Gly Asp Gin Tyr Tyr Thr Ala Tyr Ser Lys Glu Glu Leu Leu 550 555 560 gat agt ttc aaa caa att att gga ttt gaa gaa gag gtg ggg cgt tet 4425 Asp Ser Phe Lys Gin lie lie Gly Phe Glu Glu Glu Val Gly Arg Ser 565 570 575 580 tea tet cat aaa ccg aaa ttt taagattgtc caaggataac gctaaaaaat 4476

Ser Ser His bys Pro Lys Phe 585 -124- 136852·序列表.doc [Vi 1334884

ctcttagcac aggctaagag atttttttat gtgtttttca aattttatct actggtgatt 4536 ttaattcatt acataacata actttttcgt gaataataca gaatagacaa caataagaat 4596 taaaacgctc aaggcataga ggcttagtcg aacaaactaa gctattttgc gcgattgatt 4656 gggatataga tgttatttca aataagcaat aaccatggta ctgagaaaga agatgagtgc 4716 cgtaataaag tagaagcgat ttttcttttg gctgaaaggt ggtgttagcc tttttcggct 4776 aaagaaaata gtagcaacgg caatataaag ggcgataaag agcattttgt aaatgaacca 4836 tgttgttaca ttttgctgaa aaagaagaaa tagcccaatt cccgaacaga acaacagcgt 4896 atcacttaag tgcggtaaga tttttaatac ttttctgtct cgccaatttt tgcctgttaa 4956 ttgcatcatg ccacggataa taaatagact gaggctgaga aaggcacagg caatgtgtag 5016 ataaataaga taatatgcca tcgatttttt tcctaagata aagaaagcag tagcgaggct 5076 actgctttaa aataatatcg tatttagtga atgaatttaa ctttctgcgc tttcgaccgc 5136 actttcgctc gcatcactct caatgtcttc ttcatcgaca tcacaaacga gttgtaatcc 5196 aaccagtgtt tcactttcac ttgttcggat gagacgtact ccttgtgtat ttcgaccgat 5256 aatattgatt tcgtttacgc gagtacgtac tagcgtaccg gcatcagtaa tgagcataat 5316 ttgatcgctt tcctcaactt gtgttgctgc aacgacttta ccgttacgct cactcacttt 5376 aatcgagatc acgcctttgg tgttacgtga tttagttggg tattccgcta attcagtacg 5436 tttaccataa ccgttttgtg tggcggttaa aatggcgcct tcattttttg gaataacaag 5496 tgacacgact ttatcaatat tgagatccaa ggcatcatta gtattctcat cagagatctc 5556 ttccatatcg accgcacttt catcatccga caaatcattc gttaacgcca gtttaatacc 5616 gcgtacacct gttgctgcac gccccatggc acgcacagca ttttcactaa agcgaactac 5676 acgtccttgt gcggagaaga gcatgatttc attttgacca tcggtgatat ccacaccgat 5736 aagttcgtct tcatcacgca agttgagtgc aataatgcct gttgaacgag gacgactgaa 5796 tt 5798 <210> 59 <211> 587 <212:»蛋白質 <213:»欧血性巴《德桿菌 <400> 59 Met Lys Lys 1 Phe Tyr Glu

Ala Phe Pro 35 Vai Val Leu

Leu Tyr Leu 5 Asn Glu Leu 20 Leu Leu Val Asp Lys Ala lie Arg Ser Cys 10 Gly Val Tyr Thr 25 Leu He Gly Phe 40 Arg Leu Ala Gin

Tyr Asp Ser Val Arg Lys 15 Val Met Thr Ala Leu Leu 30 Thr Val Asp Gly Thr Gly 45 Gly Met Asp Gin Ala Ala •125- 136852-序列表.doc i si 50 55 60

Leu Ala Leu Val Ala Glu Asn Asn Asp Tyr Arg Gin Asn Lys Lys Hi5 65 70 75 80

Gly Asp val Asn Arg Gin Val Val Ser Pro Gin Asp Lys Ala Lys Phe 85 90 95

Gly Gly Asn Glu Phe Met Ala Lys Gin Glu Lys Arg Asn Gin Glu Leu 100 105 110 lie Gin Gly lie Ala Lys Leu Tyr Leu Arg Ser Glu Asn Ala Asn Ala 115 120 125

Ser Ser Asp Ala Pro lie Thr He Asp Lys Pro Phe His Tyr Ser Cys 130 135 140

Glu Glu Leu Asp Leu Pro Thr Ala Asn Glu Tyr Ala Arg Arg Lys Pro 145 150 155 160 lie Val Cys Glu Val Gin Gly Gly Val Asn Arg Lys Phe Trp Leu Pro 165 170 175

Val Ser Glu Ser Leu Val Ser Glu Asp Lys Leu Lys Lys Asp Arg Val 180 185 190

Arg Leu Glu Ser Asp Thr Ser Tyr Ala lie Lys Glu Lys Gly lie Val 195 200 205 lie Pro Val Glu Leu Met Leu Val Ser Asp Tyr Ser Gly Ser Met Asn 210 215 220

Ser His Leu Gin Asp Lys Asn Gly Arg Ser Leu Gly Lys Ala Lys lie 225 230 235 240

Thr lie Leu Arg Glu Val Val Ser Glu lie Ser Lys lie beu Leu Pro 245 250 255

Glu Asp Val Ser Glu Gly Val Ser Pro Phe Asn Arg lie Gly Phe Thr 260 265 270

Thr Phe Ser Gly Gly Val Arg Gin Arg Asp Val Thr Glu Gly Cys Val 275 280 285 beu Pro Tyr Glu Gly Lys lie Ser Gin Thr Ser Arg Lys Leu Thr lie 290 295 300

Arg Tyr Trp lie Thr Gly Asn Asn Thr Pro Trp Lys Phe Asn Ala Gly 305 310 315 320

Arg Trp Glu Arg Ser Thr Val Ser Phe Gin Glu His Tyr bys Gly Tyr 325 330 335

Tyr Asp Lys Phe His Ser Ser Thr Cys Arg Gly Ser Gly Ser Ser Arg 340 345 350

Thr Cys Gin lie Asp Ala Asn Pro Lys Lys lie Met Asp Tyr Ala Leu 355 360 365

Lys lie Asn Asp Trp Thr Thr lie Arg Glu Leu Phe Asn Thr Tyr lie 370 375 380

Asp Val Ser Gly Thr lie Asp Gin lie Ser Gin Phe Asp Gly Ser Asn •126- 136852·序列表.doc

l SI 385 390 395 400

Arg Arg Tyr Asp Met Val Phe Thr Asp Glu Glu Arg Cys Leu Gly Gly 405 410 415

Asn lie Gly Arq Arg 丁hr Thr Arg Ala Trp Phe Asp Gin Lys Asn Lys 420 425 ' 430

Asp lie Thr Arg Glu Leu Asn lie Val Arg Pro Ser Gly Trp Thr Ser 435 440 445

Ala Ser Ser Gly Leu Leu Val Gly Ala Asn lie Met Met Asp Glu Asn 450 * 455 460

Lys Asn Pro Asp Ala Gin Pro Ser Lys Leu Gly Thr Asn He Gin Arg 465 470 475 480

Val lie Leu Val Leu Ser Asp Gly Glu Asp Asn Trp Pro Thr Tyr Ser 485 * 490 495

Thr Leu Thr Thr Leu Leu Asn Asn Gly Met Cys Asp Lys lie Arg Glu 500 505 510

Gin Leu Gly Lys Leu Gin Asp Pro Asn Leu Arg Glu Leu Pro Gly Arg S15 520 525

He Ala Phe Val Ala Phe Gly Tyr Ser Pro Pro Ala Asn Gin Val Ala 530 535 540

Ala Trp Lys Lys Cys Val Gly Asp Gin Tyr Tyr Thr Ala Tyr Ser Lys 545 550 555 560

Glu Glu Leu Leu Asp Ser Phe Lys Gin lie lie Gly Phe Glu Glu Glu 565 570 575

Val Gly Arg Ser Ser Ser His Lys Pro Lys Phe 580 585

<2X0> 60 <211> 5798 <212> DNA <213>敗血性巴斯德桿菌 <220>

<221> CDS <222> (69Θ) . . (1468) <220> <223>未知的D2 <400> 60 gtcgacaaga ataaaacgaa acagcaaata gaattagaat tactacttat taacaataat 60 ccttttttaa agattctagc tgttttagat aaaaatatac gtgtgaagct tttggtcaca 120 ttattattat caggcctata ttatctatat gcagtaaaaa acgattcaaa ttttgtcata 180 ggtttatcta ttattttagt ttttattatt gtcattccag ggattttgac aaatgctatt 240 ttgaaagcta aggcgaaaaa aatcatggta gatttaccag gttttactaa cttagttgca 3〇〇 gtaaatgttc aaacagggat taqtattqat qcggctctaa aacaaataac aatcaatttt 360 127- Π6852·序列表.doc aagaaactta atccagatct tacttatgtg atgttaagga ttattagaaa atctgaactt acgggactac cacaagcgtt acaggatctt ccgatctcat tgccaacaac agaaataaga atgttttgta ctgctttaca acagagttta aattttggtt cttcaattta ttctcactta attcagttgt ctgcagatat cagggagata caatCattaa tcattgagga aaagttaggt acattatcag ctaaaatgag tatcccattg attttgttta ttatgttccc aataatcatt ttaattctag caccaggtat aatgagggta 'ttccaa atg ttt ttt aaa ttt acc

Met Phe Phe Lys Phe Thr 1 5 aag aaa ate gtt ttt gtt agt tta get tta tet gtc gtt ggt tgt tet

Lys Lys lie Val Phe Val Ser Leu Ala Leu Ser Val Val Gly Cys Ser 10 15 20 acc cat tet cag caa ggc atg aca cag aaa agt atg tea tet gaa aca

Thr His Ser Gin Gin Gly Met Thr Gin Lys Ser Met Ser Ser Glu Thr 25 30 35 ata aeg gca aaa gag act tta tat gaa agt aeg caa aat tat teg gca lie Thr Ala Lys Glu Thr Leu Tyr Glu Ser Thr Gin Asn Tyr Ser Ala 40 45 50 etc att tea ctg cat ege gat gtg ttg aaa gee aaa gaa gat cct tea

Leu He Ser Leu Tyr Arg Asp Val Leu Lys Ala Lys Glu Asp Pro Ser 55 60 65 70 ata ege tat aaa tta geg aag aca tac tat cag ega ggt gac age aaa lie Arg Tyr Lys Leu Ala Lys Thr Tyr Tyr Gin Arg Gly Asp Ser Lys 75 80 85 tet tet tta ett tat tta aeg cca tta ctg aat gat aat aeg aag ett

Ser Ser Leu Leu Tyr Leu Thr Pro Leu Leu Asn Asp Asn Thr Lys Leu 90 95 100 get aca caa geg aaa ata tta cag ata aaa aat eta att caa tta aat

Ala Thr Gin Ala Lys lie Leu Gin lie Lys Asn Leu lie Gin Leu Asn 105 110 115 aat ttc caa gaa gca att tet gtc gca aat gaa etc tta tta'aaa tea

Asn Phe Gin Glu Ala He Ser Val Ala Asn Glu Leu Leu Leu Lys Ser 120 125 130 cct aat gaa gga gaa gta tat aat tta aga ggt ate get tat geg caa

Pro Asn Glu Gly Glu Val Tyr Asn Leu Arg Gly He Ala Tyr Ala Gin 135 140 145 150 aat ggg aat ttg gtg aat gee ega aat gat ate aat aaa gca aga gag

Asn Gly Asn Leu Val Asn Ala Arg Asn Asp lie Asn Lys Ala Arg Glu 155 160 165 ttc ttt att aat gat aat gtt get att aat aat tta gee atg eta aat

Phe Phe lie Asn Asp Asn Val Ala lie Asn Asn Leu Ala Met Leu Asn 170 175 180 att att aat ggc gat ttt aat aat get gtt tet tta ctg ttg cca caa lie lie Asn Gly Asp Phe Asn Asn Ala Val Ser Leu Leu Leu Pro Gin 185 190 195 tat cca aat ggc gtt aag aat tet ega ttg att cat aat ett gtt ttt 136852-序列表.doc -128- 420 480 540 600 660 715 763 811 859 907 955 1003 1051 1099 1147 1195 1243 1291 1339 131 1334884

Tyx i^eu /vsn cuy vai bys Asn Ser Arg beu lie His Asn Leu Va上 Phe 200 20S 210 get tta gtt aaa aat ggt gat ett gat tat gca aaa gat ate att gtt 1387 Ala Leu Vai Lys Asn Gly Asp Leu Asp Tyr Ala Lys Asp lie He Val 215 220 225 230 aaa gag cgt tta aat act tea cca gat gat tta att aat gca ttg aaa 1435 Lys Giu Arg Leu Asn Thr Ser Pro Asp Asp Leu lie Asn Ala Leu Lys 235 240 245

aaa act aca cat gta tea aaa ggt gta act egg Lys Thr Thr His Val Ser Lys Gly Val Thr Arg 250 255 gaaaaagttt ctatcaaata taaaaggaac ctcgtcaatt gttctattta tttgttgtga tgtttatttt tgaattttgt ttattgggat ttagctataa cggaaagtgt cagaattgcg tggaaattat gaagaageat ttaggaaagc tcttacaaag atcgacaatt ggatatttgg cgttgttaga agataataaa tgtggattgt gataaagaaa eggaatgtat taaaaatctt accacaaaaa aatcataaag gagagtcaat ctctcctacg acaatattct ttaaettata aatataagtt tatggtgccg ttggtctcaa gtagtgctga accgtgaatt tgttgttgta atttatgtta ggagcaaaac caagttctct tgggacgaat atttegaget atatatgaaa gagtcaggtt tagttaaatt aaaagggcgc agtgacgata gagttccttt ttatgtcaat catttctctt cgatttagta atgttacgtt ctacattagg atacattagt tagtattctc cgtgaacgta cacagttgta atattgatga tcataagcaa tttgaaaagc ttgctaagaa atagtaataa aaggategat gttgttttag aatattgggc ggattccaaa tatcattggc gattgtaege cttacaaaaa tatctcctcg ctcagaactc aataatgaaa gaaaaatacc gtgttgaaac tcagggcttg tttgaaacaa tattactgga ggctgattag atcatcgtca atgteagtat cacgataaat aaaaaacttt atttaatteg ttettgetat gattcagtca ctaggtgttt atacagtaat gactgcatta etageattte tttacggttg atggaactgg ggttgtgctc gataaageae caagctgctt cagctttggt tgctgaaaac aatgactacc taacactaag gatttgatat 1488 gaatttgctt egattagegg aagaatgaac caaaaaaaat tttgatgtaa ctgcttaata gggagtcgcg ttagtattta caggaatttg ccatagaaaa caagcatttt gtttctgatt caagttagat egatagagtt actgatttat acaagacggt actttctgat gctttatcaa taagtctgag tategttagg gaaaatttta cattattagt gtttagctca gagaaaataa tgaegatage 1S48 ttgcgacagc 1608 aagcaatttc 1668 tccatgatga 1728 aagtccaata 1788 aatttcgcca 1848 egaetttage 1908 ttcctgagtc 1968 agegttetea 2028 tttactcatt 2088 tggaaaaata 2148 gtgctatttg 2208 aatgcctcat 2268 gcacaaatta 2328 ggtgatcaga 2388 tctggacgga 2448 ttatcctatt 2508 attactcttt 2568 cgttcaacgg 2628 gaactttatg 2688 tgagaatgag 2748 tttgattgga 2808 aggaatggat 2868 aaaacataat 2928 136852·序列表.doc •129· izi 1334884 gatgttaatc ggcaagtagt atcgcctcaa gacaaagcaa aatttggtgg taatgaattt 2988 atggcgaaac aagaaaagcg taatcaagag cttatccagg gtattgccaa actttattta 3048 cgttcagaaa atgcgaatgc ttcatctgat gcaccaatca ctattgataa accttttcat 3108 tattcatgtg aggagttaga tttacctaca gctaatgagt atgcacgtcg taaacctatt 3168 gtttgtgaag tgcaaggtgg cgtcaatcgt aaattttggc ttcctgtcag tgaatcgtta 3228 gttagtgaag ataaactgaa aaaagatcga gttagactgg aacccgatac cagttatgcg 3288 attaaagaaa aaggcatcgt gattcctgtg gagctaatgc ttgtttcgga ttattctggt 3348 tcgatgaata gtcatttaca ggataaaaac ggtagatctc taggaaaagc taaaattact 3408 attttaagag aagtggttag tgaaatttcg aaaattttat tgccagaaga tgttagcgaa 3468 ggtgtgagcc ctttcaaccg tattggcttt acgacttttt ctggcggtgt tagacaacgg 3528

gatgtcactg ttaactattc tgggagagaa tcttcaactt aaaattatgg acttatatag cgttatgata acaactcgag cgtccttctg gacgagaata atcttagtat ttaaacaatg ttacgagagt caagttgccg gagttgttag tctcataaac gctaagagat ataacataac ggcatagagg ttatttcaaa gaagcgattt aqcaacqgca aaggctgtgt gttattggat gtacagtgtc gtagaggctc attatgcact atgtaagtgg tggtgtttac cttggtttga gttggacttc agaatcctga tatctgatgg gtatgtgtga taccaggaag cttggaaaaa atagtttcaa cgaaatctta ttttttatgt tttttcgtga cttagtcgaa taagcaataa ttcttttggc atataaaqqq gcttccatat tacgggtaat tttccaggag agggagctct aaaaattaat gacgattgac tgatgaagaa tcaaaaaaat tgcatcttcg tgcgcaacct tgaagataac caaaattcga aattgcgttt atgtgtaggt acaaattatt agattgtcca gtttttcaaa ataatacaga caaactaagc ccatggtact tgaaaggtgg cqataaaqaq gaaggaaaaa aatacacctt cattataaag agaacttgtc gactggacga caaatttccc cggtgtcttg aaagatatta gggcttcttg tcgaaactcg tggccaactt gaacaattgg gttgcgtttg gatcaatatt ggatttgaag aggataacgc ttttatctac atagacaaca tattttgcgc gagaaagaag tgttagcctt cattttgtaa tatcacaaac ggaaatttaa gctattatga aaattgatgc caattagaga agtttgatgg gcggaaatat caagagagtt ttggagctaa ggacaaatat atagtacatt gcaagttaca gctatagtcc atacggctta aagaggtggg taaaaaatct tggtgatttt ataagaatta gattgattgg atgagtgccg tttcggctaa atgaaccatq ttctcgaaaa 3588 tgctgggaga 3648 caaattccat 3708 aaatcctaag 3768 attatttaat 3828 ttcaaacaga 3688 tggtagaaga 3948 gaatattgtt 4006 tatcatgatg 4068 tcaacgtgtt 4128 aacgactctt 4188 agatccaaat 4248 accagcaaac 4308 ttcgaaagaa 4368 gcgttcttca 4428 cttagcacag 4488 aattcattac 4546 aaacgctcaa 4608 gatatagatg 4668 taataaagta 4728 agaaaatagt 4788 -130- 136852-序列表.doc I Si 1334884 ttgctgaaaa agaagaaata gcccaattcc cgaacagaac aacagcgtat cacttaagtg 4908 cggtaagatt tttaatactt ttctgtctcg ccaatttttg cctgttaatt gcatcatgcc 4968 acggataata aatagactga ggctgagaaa ggcacaggca atgtgtagat aaataagata 502Θ atatgccatc gatttttttc ctaagataaa gaaagcagta gcgaggctac tgctttaaaa 5088 taatatcgta tttagtgaat gaatttaact ttctgcgctt tcgaccgcac tttcgctcgc 5148 atcactctca atgtctcctt catcgacatc acaaacgagt tgtaatccaa ccagtgtttc 5208 actttcactt gttcggatga gacgtactcc ttgtgtattt cgaccgataa tattgatttc 5268 gtttacgcga gtacgtacta gcgtaccggc atcagtaatg agcataattt gatcgctttc 5328 ctcaacttgt gttgctgcaa cgactttacc gttacgctca ctcactttaa tcgagatcac 5388 gcctttggtg ttacgtgatt tagttgggta ttccgctaat tcagtacgtt taccataacc 5448 gttttgtgtg gcggttaaaa tggcgccttc attttttgga ataacaagtg acacgacttt SS08

atcaatattg agatccaagg catcattagt attctcatca gagatctctt ccatatcgac 5568 cgcactttca tcatccgaca aatcattcgt.taacgccagt ttaataccgc gtacacctgt 5628 tgctgcacgc cccatggcac gcacagcatt ttcactaaag cgaactacac gtccttgtgc 5688 ggagaagagc atgatttcat tttgaccatc ggtgatatcc acaccgataa gttcgtcttc 5748 atcacgcaag ttgagtgcaa taatgcctgt tgaacgagga cgactgaatt 579.8 <210> 61 <211> 257 <212>蛋白筲 <213>敗血性巴斯谂桿菌 <400> 61

Met Phe Phe Lys Phe Thr Lys Lys lie Val Phe Val Ser Leu Ala Leu 15 10 15

Ser Val Val Gly Cys Ser Thr His Ser Gin Gin Gly Met Thr Gin Lys 20 25 30

Ser Met Ser Ser Glu Thr lie Thr Ala Lys Glu Thr Leu Tyr Glu Ser 35 40 45

Thr Gin Asn Tyr Ser Ala Leu lie Ser Leu Tyr Arg Asp Val Leu Lys 50 55 60

Ala Lys Glu Asp Pro Ser lie Arg Tyr Lys Leu Ala Lys Thr Tyr Tyr 65 70 75 80

Gin Arg Gly Asp Ser Lys Ser Ser Leu Leu Tyr Leu Thr Pro Leu Leu 85 90 95

Asn Asp Asn Thr Lys Leu Ala Thr Gin Ala Lys lie Leu Gin lie Lys 100 105 110

Asn Leu lie Gin Leu Asn Asn Phe Gin Glu Ala lie Ser Val Ala Asn 115 120 125 • 131 - 136852-序列表.doc i Si 1334884

Giu Jjeu Leu beu Lys Ser Pro Asn Glu Gly Glu Val Tyr Asn Leu Arg 130 135 140

Gly lie Ala Tyr Ala Gin Asn Gly Asn Leu Val Asn Ala Arg Asn Asp 145 150 155 160 lie Asn Lys Ala Arg Glu Phe Phe lie Asn Asp Asn Val Ala lie Asn 165 170 175

Asn Leu Ala Met Leu Asn lie He Asn Gly Asp Phe Asn Asn Ala Val 180 185 190

Ser Leu Leu Leu Pro Gin Tyr Leu Asn Gly Val Lys Asn Ser Arg Leu 195 200 205 lie His Asn Leu Val Phe Ala Leu Val Lys Asn Gly Asp Leu Asp Tyr 210 215 220

Ala Lys Asp lie He Val Lys Glu Arg Leu Asn Thr Ser Pro Asp Asp 225 230 235 240

Leu lie Asn Ala Leu Lys Lys Thr Thr His Val Ser Lys Gly Val.Thr

245 250 255

Arg

<210> 62 <211> 1788 <212> DNA <213>敗血性巴斯德桿菌 <220> <221> CDS <222> (1) . . (600) <220> <223> 未知的K <400> 62 gtc aac act ggg eta att cac agt aac ggt aat gcc aag etc act ttt Val Asn Thr Gly Leu lie His Ser Asn Gly Asn Ala Lys Leu Thr Phe 15 10 15 aaa gat gac acc agt ttt gtg act gaa gga aat aac ttt ate aca gca Lys Asp Asp Thr Ser Phe Val Thr Glu Gly Asn Asn Phe lie Thr Ala 20 25 30 aaa gac aac tta gaa ate aeg gca aaa aat gtt caa att gat caa geg Lys Asp Asn Leu Glu lie Thr Ala Lys Asn Val Gin lie Asp Gin Ala 35 40 45 aaa aat att caa tta aac geg aat ate aeg ate aat acc aag tet ggt Lys Asn lie Gin Leu Asn Ala Asn lie Thr lie Asn Thr Lys Ser Gly 50 55 60 ttt gtg aat tac ggt acc tta gca agt get caa aat tta aeg att aat Phe Val Asn Tyr Gly Thr Leu Ala Ser Ala Gin Asn Leu Thr lie Asn 65 70 75 80 acc gaa caa ggc age att tat aac ata ggc ggt ate ttg ggg geg ggt Thr Giu Gin Gly Ser lie Tyr Asn lie Gly Gly lie Leu Gly Ala Gly 48 96 144 192 240 132· 136852·序列表.doc 288 1334884 85 90 95 aaa agt ttg aat ctg age geg aaa aga gga gaa aac caa gga gga tat 336

Lys Ser Leu Asn Leu Ser Ala Lvs Arg Gly Gl'i Asn Gin Gly Gly Tyr 100 - 105 110 ' ett att aat caa ggt aaa agt eta etc cat tet gaa ggc gee atg r*ac 384 ben lie Asn Gin Gly Lys Ser Leu Leu His Ser Glu Qly Ala Met Λεη 115 120 125 etc aca geg gat ege aeg gtg tac aat tta ggg aat att ttt get aaa 432

Leu Thr Ala Asp Arg Thr Val Tyr Asn Leu Gly Asn lie Phe Ala Lys 130 135 140 ggt gac geg aeg ate aat gca aac geg tta att aat gat gtt act etc 480

Gly Asp Ala Thr lie Asn Ala Asn Ala Leu lie Asn Asp Val Thr Leu 145 150 155 160 aca ggt cgt ett gag tat caa gat ctg aaa aaa gat tat aeg cgt tat 528

Thr Gly Arg Leu Glu Tyr Gin Asp Leu Lys Lys Asp Tyr Thr Arg Tyr 165 170 175

tat cgt ate aat gaa aeg gca aaa cat ggt tgg cat aat aac ttc tat 576

Tyr Arg He Asn Glu Thr Ala Lys His Gly Trp His Asn Asn Phe Tyr 180 185 190 gaa tta aac gtc gac aga gtt tet tgatttgtgc atcaattttg taaccaccgg 630 Glu Leu Asn Val Asp Arg Val Ser 195 200

ttaataaaac catcaggacg tggtcaaatt catgaataat ctgcgctcca tctcagatac gagtacgacc tagggttatt ccggtgtttc tttcatagtt cgacaatttc catcagattg tttcaatggt agttcggctg gtgttaaacc ttgtacgtga qaatcqgcag accagcaatt atccgcggaa acgtgcacag ggcagcacct aggaatacat ttgattttgt agattcatca tttgctgcca cgtcgccggt aatgctattg attgtttttg attttgaccg ggtactggtg agaaattggt taagctgaca cataataaac ttaattqatc tcaacgccat gtgacaagta aaagtgatgc aaatgtttgg gccaagattt gtgtgttgga atcggcgcat aataatgagg gctgcaacaa gcataagaat gcgagttgtt atgagtgatt egaataattg ttcataaagc ctggttaagc cctaatttgt tttacqcqat teatggeaga aacttccaac cacgaatgcg ctaaatcaat taattgggct aagaatcaaa taaatttatt ctgcggcttt gaatgatttc gettaegegt gatgattttc cagctacact atgttgtgcg cgactttcgc ctacaccagc tgataattta acaaaaqcqc taatgeeget gcggaaatgt acgttcattg ggcacgagtc tttctcaaat aatttctgcc gatcacaaca gatgegttet cgcatcaagt agggattaaa aacaattttt taacataaat atcaatcata ccctttttgc actaatcggg tacaaaaaga qcac3t：flt:ctt： gegaegatea 690 tccaccatat 750 atcgcgcctt 810 gcaattaatt 870 aaatgataaa 930 aagtcagggc 990 ccaagtaaat 1050 ttgagttctg 1110 gettgageaa 1170 ccttccacca 1230 tctagtacca 1290 ggttcactgg 1350 tcttcacctg 1410 tccagtgcat 1470 ataaggataa 1530 aactgccgat 1590 •133· 13胡52-序列表.doc i si 1334884 aagttcttca ttcgttggga tcaccatggc aacaggcgta ttgtctgctg taatcacccc ttcatgacca aagcgagccg ctttgttttt atctgaatcc acttgataac cgaacagttt taaatggttt aaggttga <210> 63 <211> 200 <212>蛋白質 <213>敗血性巴斯德桿菌 <400> 63

Val Asn Thr Gly Leu lie His Ser Asn Gly Asn Ala Lys Leu Thr Phe 1 5 10 15

Lye Asp Asp Thr Ser Phe Val Thr Glu Gly Asn Asn Phe lie Thr Ala 20 25 30

Lys Asp Asn Leu Glu lie Thr Ala Lys Asn Val Gin lie Asp Gin Ala 35 40 45

Lys Asn lie Gin Leu Asn Ala Asn lie Thr He Asn Thr Lys Ser Gly 50 55 60

Phe Val Asn Tyr Gly Thr Leu Ala Ser Ala Gin Asn Leu Thr lie Asn 65 70 75 80

Thr Glu Gin Gly Ser lie Tyr Asn lie Gly Gly He Leu Gly Ala Gly 85 90 95

Lys Ser Leu Asn Leu Ser Ala Lys Arg Gly Glu Asn Gin Gly Gly Tyr 100 105 110

Leu lie Asn Gin Gly Lys Ser Leu Leu His Ser Glu Gly Ala Met Asn 115 120 125

Leu Thr Ala Asp Arg Thr Val Tyr Asn Leu Gly Asn lie Phe Ala Lys 130 135 140

Gly Asp Ala Thr lie Asn Ala Asn Ala Leu lie Asn Asp Val Thr Leu 145 ISO 155 160

Thr Gly Arg Leu Glu Tyr Gin Asp Leu Lys Lys Asp Tyr Thr Arg Tyr 165 170 175

Tyr Arg lie Asn Glu Thr Ala Lys His Gly Trp His Asn Asn Phe Tyr 180 185 190

Glu Leu Asn Val Asp Arg Val Ser 195 200

<210> 64 <211> 278 <212> DNA <213>敗血性巴斯揀桿菌 <220>

<221> CDS <222> (108) . - 1278) <220> -134- 1710 1770 1788 136852-序列表.doc 1334884 <223>表知的〇 <400> 64 gaattccaac caaatctcac accagagcaa gaacgctaca tagtggaatg gttggcagaa 60 cattacccaa atggaaataa accttaacca tagcaagaga gaagaaa atg aaa att 116

Met Lys lie 1 act att aca cga aat cat cca gaa gta ttt caa gaa tcc get cgt tta 164

Thr lie Thr Arg Asn His Pro rlu Val Phe Gin Glu Ser Ala Arg Leu 5 10 15 gta gcc gaa aag ttc att aaa gcc caa tgt gta gaa gca tta aca ttg 212

Val Ala Glu Lys Phe lie Lys Ala Gin Cys Val Glu Ala Leu Thr Leu 20 25 30 35 get ttg att gag ggt gtc gag cac ttt gtg ctg gaa ggt gag gag gaa 260

Ala Leu lie Glu Gly Val Glu His Phe Val Leu Glu Gly Glu Glu Glu 40 45 50

age aaa agg gga cat agt 278

Ser Lys Arg Gly His Ser 55 <210> 65 <211> 57 <212>蛋白質 <213>敗血性巴斯德样菌 <400> 65

Met Lys lie Thr He Thr Arg Asn His Pro Glu Val Phe Gin Glu Ser 1 5 10 15

Ala Arg Leu Val Ala Glu Lys Phe lie Lys Ala Gin Cys Val Glu Ala 20 25 30

Leu Thr Leu Ala heu lie Glu Gly Val Glu His Phe Val Leu Glu Gly 35 40 45

Glu Glu Glu Ser Lys Arg Gly His Ser 50 55

<210> 66 <211> 1020 <2X2> DNA <213>敗血性巴斯德桿菌 <22Q> <221> CDS <222> (1)..(597) <220> <223>未知的P <400> 66 gtc aac aca tea aaa gtt gag att gac tat gcc gtc act cgt geg geg 48

Val Asn Thr Ser Lys Val Glu lie Asp Tyr Ala Val Thr Arg Ala Ala 1 ' 5 l〇 15 gca atg cgt gca tat ett gac aaa gaa cag ggc tgg cat aeg tet att 96 • 135- 136852·序列表.doc i Ti 1441334884

Ala Met Arg Ala Tyr Leu Asp Lys Glu Gin Gly Trp His Thr Ser lie 20 25 30 tea aat aaa ggc att aat ggc gtg age ggt gtc aca caa cca etc tat

Ser Asn Lys Gly lie Asn Gly Val Ser Gly Val Thr Gin Pro Leu Tyr 35 40 45 ttt gac att aac gac age teg act gat gtg aac tat etc aat gaa caa

Phe Asp lie Asn Asp Ser Ser Thr Asp Val Asn Tyr Leu Asn Glu Gin 50 55 60 ggc ate aeg tgt tgc gtg aat cat aat ggc ttt cgt ttt tgg ggc tta

Gly lie Thr Cys Cys Val Asn His Asn Gly Phe Arg Phe Trp Gly Leu 65 70 75 80 ege aeg act gca gaa gat cca tta ttc aag ttt gaa gtg tac acc ege

Arg Thr Thr Ala Glu Asp Pro Leu Phe Lys Phe Glu Val Tyr Thr Arg 85 90 95 act gca caa ate tta aaa gat aeg att gca ggg geg ttt gat tgg gca

Thr Ala Gin lie Leu Lys Asp Thr lie Ala Gly Ala Phe Asp Trp Ala 100 105 110 gtg gat aaa gac att tet gtc aeg eta gtg aaa gat att att gaa gca

Val Asp Lys Asp lie Ser Val Thr Leu Val Lys Asp lie lie Glu Ala 115 120 125 ate aat geg aag tgg cgt gat tac acc aca aaa ggc tac tta att ggc lie Asn Ala Lys Trp Arg Asp Tyx Thr Thr Lys Gly Tyr Leu lie Gly 130 135 140 ggt aaa geg tgg ett aat aaa gag ett aac agt gca aeg aat tta aaa

Gly Lys Ala Trp Leu Asn Lys Glu Leu Asn Ser Ala Thr Asn Leu Lys 145 150 155 160 gat geg aag ttg ttg ate tet tat gat tat cac cca gta cca ccg etc

Asp Ala Lys Leu Leu lie Ser Tyr Asp Tyx His Pro Val Pro Pro Leu 165 170 175 gaa cag eta ggc ttt aat cag tac att tet gat gaa tac ett gtt gat

Glu Gin Leu Gly Phe Asn Gin Tyr lie Ser Asp Glu Tyr Leu Val Asp 180 185 190 ttt tea aat cgt tta gca teg taaggggtag aaaatggctt taccacgcaa Phe Ser Asn Arg Leu Ala Ser 195 acttaaattg atgaatttaa teategaegg taacaaatat ctcggcgaag teaeggaagt gactcaacca aaattagcaa tgaaaatcga agaatttege gcgggcggta tgattggttc ggtggatgtc aatctcgggc ttgaaaagct egaageggaa tttaaagccg gtggctacat ggtegaatta attaaaaaat tcggcgggtc aatcaacggc attccattgc gttttcttgg ctcatatcag cgtgatgaca cagaagaagt cacatctgtt gagcttgtga tgcaaggtcg atttactgaa attgacagcg gaaacagcaa agtgggcgat gacactgaac aaacattcaa agtgccttta acgtattaca aaatcattgt tga <210> 67 Ϊ 36852·序列表.doc •136· 192 240 288 336 384 432 480 526 576 627 687 747 807 867 927 987 1020

1334884 <211> 199 蛋白質 <213>敗血性巴斯德·桿菌 <400> 67 vral Asn Th·*- Ser Lvs Val Qlu Tie Asp Tyr Ala Val Thr Arg Ala Ala 1 、 10 15

Ala Met Arg Ala Tyr Leu Asp Lys Glu Gin Gly Trp His Thr Ser lie 20 25 30

Ser Asn Lys Gly lie Asn Gly Val Ser Gly Val Thr Gin Pro Leu Tyr 35 40 45

Phe Asp lie Asn Asp Ser Ser Thr Asp Val Asn Tyr Leu Asn Glu Gin 50 55 60

Gly lie Thr Cys Cys Val Asn His Asn Gly Phe Arg Phe Trp Gly Leu 65 70 75 80

Arg Thr Thr Ala Glu Asp Pro Leu Phe Lys Phe Glu Val Tyr Thr Arg 85 90 95

Thr Ala Gin lie Leu Lys Asp Thr lie Ala Gly Ala Phe Asp Trp Ala 100 105 110

Val Asp Lys Asp lie Ser Val Thr Leu Val Lys Asp lie lie Glu Ala 115 120 125 lie Asn Ala Lys Trp Arg Asp Tyr Thr Thr Lys Gly Tyr Leu lie Gly 130 135 140

Gly Lys Ala Trp Leu Asn Lys Glu Leu Asn Ser Ala Thr Asn Leu Lys 145 150 155 160

Asp Ala Lys Leu Leu He Ser Tyr Asp Tyr His Pro Val Pro Pro Leu 165 170 175

Glu Gin Leu Gly Phe Asn Gin Tyr lie Ser Asp Glu Tyr Leu Val Asp 1θ〇 185 190

Phe Ser Asn Arg Leu Ala Ser 195

<210> 68 <211> 2584 <212> DNA <213>敗血性巴斯谂桿兹 <220>

<221> CDS <222> (1042J ..(2286) <220> <223> xylA <400> 68 gtcgaccagc ttagattttg cgacggggtt aatttcttct atcgtttcaa tcattgcgtt 60 taccattatt ttatggaatc tctctggacc gatgaccatt gccaatattg aaattcctca 120 cgcoatqar.c tttzftqotct σ<τί;〇ι:1：ι；3α<7 catttaaaat 1«0 136852·序列表.doc -137- 1334884 cggtcgcccg ncaatccagc tcaattttgc caatgaacgc ttaaacgcca actaccgtta 240 cccacctacc cgtctgaaag aacatgctga aagcattgct tctcaccgtg gcgaaaaaat 300 ggaaaaacgt ctattgacca cacaatttaa tcaggtgatt gataacgttt ggcaagtaat 360 ctaccgcacc ttgaaattat ccggttttaa cttaatcatt acgcagattt cggtggtttt 420 cccgctggtg atccaagtga cacgtcattt tcgtcgacaa taggtgcata tgagggtgtt 480 agaatagcga tactttctgt tggaaaagta aactctttaa tataaataga aatcgcttga 540 atgattctcg ggcaaaaaat aatgtactca tttgcgatct catactgata atggcgaagt 600 aaatatcttc ttacaatatt atggtaatta tcaggtaata ccgtatagcc atagattcca 660 gttctatttt gttttgctaa ataattgatg agcatttgag gcgcaggtaa atccatatct 720 gcaacagaca ttgaaatcat atccttgccg tatttacgag taattgccca tttagcacta 780 tgacaatctg atctatcagt aaaaacatca aacaaattat ccgtcataca tgttctccaa 840

tattggattt atataaactt tagaacttga ggtagattgt tggaattgtt aaatctggta 900 tttctattac gttttttctt ttttgtgata taagccacaa taaccaataa tcttaattgt 960 taagtgaaat aacgtaattg atcctcccat tgttttacta aattatgtct ctgaaactta 1020 cttgttcagg agaaatcatt t atg tee act tac ttc gac aaa att gaa aaa 1071 Met Ser Thr Tyr Phe Asp Lys lie Glu Lys 1 5 10 gta aat tat gaa ggt gta act tea tet aat ccg ttt gca tat aag cat X119 Val Asn Tyr Glu Gly Val Thr Ser Ser Asn Pro Phe Ala Tyr Lys His 15 20 25 tat gat get aat caa gtt att tta ggt aag aeg atg get gaa cac tta 1167 Tyr Asp Ala Asn Gin Val lie Leu Gly Lys Thr Met Ala Glu His Leu 30 35 40 cgt Cta gee gtc tgt tat tgg cac act ttc tgt tgg aca ggg aat gat 1215 Arg Leu Ala Val Cys Tyr Trp His Thr Phe Cys Trp Thr Gly Asn Asp 45 50 55 atg ttc ggt gtc ggt tet ttc gat cgt tgt tgg cag aag geg agt gat 1263 Met Phe Gly Val Gly Ser Phe Asp Arg Cys Trp Gin Lys Ala Ser Asp 60 65 70 tea tta gca ggt gca aaa caa aaa gca gat ate get ttt gaa ttt ttc 1311 Ser Leu Ala Gly Ala Lys Gin Lys Ala Asp lie Ala Phe Glu Phe Phe 75 80 85 90 agt aaa tta ggc ata cct tat tat tgt ttt cat gat gtt gat gtt geg 1359 Ser Lys Leu Gly lie Pro Tyr Tyr Cys Phe His Asp Val Asp Val Ala 95 100 105 cca gaa ggt cat tea ttt aaa gaa tat ttg teg aac ttt aat aca atg 1407 Pro Glu Gly His Ser Phe Lys Glu Tyr Leu Ser Asn Phe Asn Thr Met 110 115 120 ate gat gtt tta geg cag aaa caa gaa gaa aca ggc gtc add ttg ttg 1455 lie Asp Val Leu Ala Gin Lys Gin Glu Glu Thr Gly val Lys Leu Leu 125 130 135

-138- 13砧52-序列表.doc i si tgg ggg act gca aat cgt ttt aca cac cct cgt tat acg tcc ggt get 1S03 Trp Gly Thr 140 Ala Asn Cys Phe 145 Thr His Pro Arg Tyr 150 Met Ser Gly Ala gca aca aat ccg aat cca gaa att ttt get tgg get gcc gca caa gta 1551 Ala 155 Thr Asn Pro Asn Pro lbO Glu He Phe Ala Trp Ala 165 Ala Ala Gin Val 170 ttt act gcc atg ggg gca act cag cgt tta ggt ggt gaa aat tat gtt 1599 Phe Thr Ala Met Gly Ala 175 Thr Gin Arg Leu 180 Gly Gly Glu Asn Tyr 185 Val ttg tgg gga gga cgt gaa gga tat gaa acg tta tta aac acc aat tta 1647 Leu Trp Gly Gly Arg Glu Gly Tyr Glu 190 195 Thr Leu Leu Asn Thr 200 Asn Leu aaa cag gag ega gag caa att gga cgt ttc atg caa atg gtg gtt gag 1695 Lys Gin Glu 205 Arg Glu Gin lie Gly Arg 210 Phe Met Gin Met 215 Val Val Glu cat aaa tat aaa ate ggt ttt aac ggg act ttg ctg att gaa cca aag 1743 His Lys 220 Tyr Lys lie Gly Phe 225 Asn Gly Thr Leu Leu 230 lie Glu Pro Lys cca caa gag cca acg aaa cat caa tat gac tat gat gtg geg acc gtt 1791 Pro 235 Gin Glu Pro Thr Lys 240 His Gin Tyr Asp Tyr 245 Asp Val Ala Thr Val 250 tat ggc ttt tta aag cag ttt ggt tta gaa aaa gaa ate aaa gtg aat 1839 Tyr Gly Phe Leu Lys 255 Gin Phe Gly Leu Glu 260 Lys Glu lie Lys val 265 Asn att gaa get aat cac gca aca tta get gga cac act ttc cag cat gaa 1887 lie Glu Ala Asn 270 His Ala Thr Leu Ala 275 Gly His Thr Phe Gin 280 His Glu gtc gcc atg get aca geg tta gat att ttt ggt tet att gat gca aat 1935 val Ala Met 285 Ala Thr Ala Leu Asp 290 lie Phe Gly Ser lie 295 Asp Ala Asn cgt ggt gat cca caa tta ggt tgg gat acc gat caa ttc cct aat age 1983 Arg Gly Asp 300 Pro Gin Leu Gly Trp Asp 305 Thr Asp Gin 310 Phe Pro Asn Ser gta gaa gaa aat act ttg gtc ata tat gaa att etc aaa gca ggg ggc 2031 Val 3X5 Glu Glu Asn Thr Leu 320 Val lie Tyr Glu lie 325 Leu Lys Ala Gly Gly 330 ttt aca acc ggt ggt ttt aat ttt gat get aaa ate cgt egg cag agt 2079 Phe Thr Thr Gly Gly 335 Phe Asn Phe Asp Ala 340 Lys lie Arg Arg Gin 345 Ser acg gat cct tac gat tta ttt cat gga cat att ggc geg att gat gta 2127 Thr Asp Pro Tyr 350 Asp Leu Phe His Gly His 355 lie Gly Ala lie 360 Asp Val ctt gcc tta tea eta aaa tgt geg geg aaa atg ctt gaa gag caa get 2175 Leu Ala Leu 365 Ser Leu Lys Cys Ala 370 Ala Lys Met Leu Glu 37S Glu Gin Ala tta caa aaa gtc gtc aat caa cgt tat get ggt tgg aca tea tea ctt 2223 Leu Gin 380 Lys Val Val Asn Gin 385 Arg Tyr Ala Gly Trp 390 Thr Ser Ser Leu 136852-序列表.doc 139- 1334884 ggc caa ccc get caa acc egg tcc tac cac gcg cgt ctg caa tac aga 2271

Gly Gin Leu Val Gin He Arg Ser Tyr His Ala Cys Leu Gin Tyr Arg 355 400 40^ 410 eta aca aaa gtg ett taaaacgttc cggcttacgc cagacatcta gaegattgaa 2326

Leu Thr Lys Val Leu 415 taatttcaat attgtctccg cacgtaattc aaaggcnctg tgtatgtgcg aatgatattc 2386 acaacaaagt tctgcaaaat ettgaattge gtgaggtaat ttaaagcgct gacataagcg 2446 tettgtegge atgacaccag ctttttcatg tccataatga tgtggcaata tttcttttgg 2506 tgttaaggct tttcctaaat catgacaaat tgcagcaaaa cgtaccgcac ttttgtcact 2566 gtccgtgttt tctgtcga 2584 <210> 69 <211> 415

<212>蛋白質 <213>畋血性巴斯埝桿菡 <400> 69

Met Ser Thr Tyr Phe Asp Lys lie Glu Lys Val Asn Tyr Glu Gly Val 15 10 15

Thr Ser Ser Asn Pro Phe Ala Tyr Lys His Tyr Asp Ala Asn Gin Val 20 25 30 lie Leu Gly Lys Thr Met Ala Glu His Leu Arg Leu Ala Val Cys Tyr 35 40 45

Trp His Thr Phe Cys Trp Thr Gly Asn Asp Met Phe Gly Val Gly Ser 50 55 60

Phe Asp Arg Cys Trp Gin Lys Ala Ser Asp Ser Leu Ala Gly Ala Lys 65 70 75 80

Gin Lys Ala Asp lie Ala Phe Glu Phe Phe Ser Lys Leu Gly lie Pro 85 90 95

Tyr Tyr Cys Phe His Asp Val Asp Val Ala Pro Glu Gly His Ser Phe 100 105 110

Lys Glu Tyr Leu Ser Asn Phe Asn Thr Met lie Asp Val Leu Ala Gin 115 120 125

Lys Gin Glu Glu Thr Gly Val Lys Leu Leu Trp Gly Thr Ala Asn Cys 130 135 140

Phe Thr His Pro Arg Tyr Met Ser Gly Ala Ala Thr Asn Pro Asn Pro 145 150 155 160

Glu lie Phe Ala Trp Ala Ala Ala Gin Val Phe Thr Ala Met Gly Ala 165 170 175

Thr Gin Arg Leu Gly Gly Glu Asn Tyr Val Leu Trp Gly Gly Arg Glu 180 185 190

Gly Tyr Glu Thr Leu Leu Asn Thr Asn beu Lys Gin Glu Arg Glu Gin 195 200 205 •140- 136852·序列表.doc tsi 1334884 lie CUy Arg Phe Met Gin Met Val Val Glu His Lys Tyr Lys lie GXy 210 215 220

Phe Asn Gly Thr Leu Leu lie Glu Pro Lys Pro Gin Glu Pro Thr Lys 225 230 235 240

His Gin Tyr Asp Tyr Asp Vai Ala Thr Val Tyr Gly Phe Leu Lys Gin 245 250 255

Phe Gly Leu Glu Lys Glu lie Lys Val Asn He Glu Ala Asn His Ala 260 265 270

Thr Leu hla Gly His Thr Phe Gin His Glu Val Ala Met Ala Thr Ala 275 280 285

Leu Asp lie Phe Gly Ser lie Asp Ala Asn Arg Gly Asp Pro Gin Leu 290 295 300

Gly Trp Asp Thr Asp Gin Phe Pro Asn Ser Va] Glu Glu Asn Thr Leu 305 310 315 320

Val lie Tyr Glu lie Leu Lys Ala Gly Gly Phe Thr Thr Gly Gly Phe 325 330 335

Asn Phe Asp Ala Lys lie Arg Arg Gin Ser Thr Asp Pro Tyr Asp Leu 340 345 350

Phe His Gly His lie Gly Ala lie Asp Val Leu Ala Leu Ser Leu Lys 355 360 365

Cys Ala Ala Lys Met Leu Glu Glu Gin Ala Leu Gin Lys Val Val Asn 370 375 380

Gin Arg Tyr Ala Gly Trp Thr Ser Ser Leu Gly Gin Leu Val Gin lie 385 390 395 400

Arg Ser Tyr His Ala Cys Leu Gin Tyr Arg Leu Thr Lys Val Leu 405 410 415

<210> 70 <211> 3501 <2X2> DNA

<213〉敗血性巴斯德桴茵 <220>

<221> CDS <222> (298) . . (1905) <220> <223> yabk <400> 70 gaattcgagg aagggggcgt attacaaatt gaaacggctg cacgtgtagc acaacatgat 60 aatgcctgtg cggatcattt ccttgccttt ttacttcatc cagaagcaca agggcattta 120 gccaagaata atgtgatgtt accggtgatt aataccaata ttgaaccgca cttngatgcc 180 cttagagcca cccaaatgaa cacgaaagtg ctcgatacct caaaagtgaa tgccgaacaa 240 gtcaaaaaat ggattgccgt ttggcaaacg accctaaccc aataattgct tgtcccg 297 -141 - 136852·序列表.doc 1 Si 1334884

atg tcc aag cga ttt cgt gca ttc aca tac cgt ccc gcc agt tat Ctt 345 Met Phe Lys Arg Phe Arg Ala Phe Thr Tyr Arg Pro Ala Ser Tyr Leu 5 Ιϋ lb ggc ggg atg ttg gtg att get ttt ctg age get ttt tat geg ttc gcc 393 Gly Gly Met Leu Val lie Val Phe Leu Ser Ala Phe Tyr Ala Phe Ala 20 25 30 tta ggg geg get ttt teg ccc cat tcc geg ege agt tgg aca geg ttg 441 Leu Gly Ala Val Phe Ser Leu Pro Phe Ala Arg Ser Trp Thr Ala Leu 35 40 45 ttg agt gat cag tat tea caa cac gtg ate ate ttt age ttt tgg caa 4Θ9 Leu Ser Asp Gin Tyr Leu Gin His Val lie lie Phe Ser Phe Trp Gin 50 55 60 gcc ttt ctg teg geg gta Ctt geg gtc etc ttt ggt ggc att gta gca 537 Ala Phe Leu Ser Ala Val Leu Ala Val Leu Phe Gly Gly lie Val Ala 65 70 75 80 cga gcc ttt ttt tat caa ccg ttt gtg 99c aag aaa ctg ate etc aaa 5B5 Arg Ala Phe Phe Tyr Gin Pro Phe Val Gly Lys Lys Leu lie Leu Lys 85 90 95 tta ttt tea ctg act ttt gtg tta cct gcc tta gtg geg att ttt ggt 633 Leu Phe Ser Leu Thr Phe Val Leu Pro Ala Leu Val Ala lie Phe Gly 100 105 110 tta tta ggc gtg tat ggc get tet ggc tgg tta geg atg tta age cag 681 Leu Leu Gly Val Tyr Gly Ala Ser Gly Trp Leu Ala Met Leu Ser Gin 115 120 125 ttt ttc get tgg gac tgg act cct aat att tac ggc tta aca ggt att 729 Phe Phe Ala Trp Asp Trp Thr Pro Asn lie Tyr Gly Leu Thr Gly lie 130 135 140 tta ctg geg cat ett ttt ttt aat gtc cca tta get tgt ege ctg ttt 777 Leu Leu Ala His Leu Phe Phe Asn Val Pro Leu Ala Cys Arg Leu Phe 145 150 155 160 tta caa ggt ttg caa gca att ccg gtg caa caa cgt cag etc geg gca 825 Leu Gin Gly Leu Gin Ala lie Pro Val Gin Gin Arg Gin Leu Ala Ala 165 170 175 caa etc aat tta cgt ggt tgg cat ttt ata cgt ctg att gag tgg ccc 873 Gin Leu Asn Leu Arg Gly Trp His Phe lie Arg Leu lie Glu Trp Pro ISO 18S 190 tat tta ege cag caa ttg tta cct gca ctt acc ttg att ttc atg ctg 921 Tyr Leu Arg Gin Gin Leu Leu Pro Ala Phe Thr Leu lie Phe Met Leu 195 200 205 tgt ttt acc agt ttt geg att gtg etc act tta ggt ggc gga ccg aaa 969 Cys Phe Thr Ser Phe Ala lie Val Leu Thr Leu Gly Gly Gly Pro Lys 210 215 220 tat acc aeg ctg gaa gtg get ate tat caa geg att tta ctt gag ttt 1017 Tyr Thr Thr Leu Glu Val Ala lie Tyr Gin Ala lie Leu Phe Glu Phe 225 230 235 240 gac gta ccg aaa gcc ggc tta ttt geg cca tta caa ttt get ttt tgt 1065 Asp Val Pro Lys Ala Gly Leu Phe Ala Leu Leu Gin Phe Val Phe Cys 245 2S0 255 136852-序列表.doc -142- IS1 1334884

ttt ctg tta ttc a eg ctg agt age ttt ttt tet cca gcc CCC gcc aeg 1113 Phe Leu Leu Phe Thr Leu Ser Ser Phe Phe Ser Pro Ala Pro Ala Thr 260 265 270 aca tta cac agt caa cct act tgg ttt geg CCC caa teg tat tgg gtt 1161 Thr Leu His Ser Gin Pro Thr Trp Phe Ala Pro Gin Ser Tyr Trp Val 275 280 285 aaa tta tgg caa cgt atg ate att gtg tgt geg aca gta ttt ate tta 1209 Lys Leu Trp Gin Arg Met lie lie Val Cys Ala Thr Val Phe He Leu 290 295 300 tta ccg eta etc aat aeg eta gtt tet get ttg ett teg tet cag ttt 1257 Leu Pro Leu Leu Asn Thr Leu Val Ser Ala Leu Leu Ser Ser Gin Phe 305 310 315 320 ttt acc ttg tgg tta caa cct caa Cta tgg aaa gca tta ggt tac teg 1305 Phe Thr Leu Trp Leu Gin Pro Gin Leu Trp Lys Ala Leu Gly Tyr Ser 325 330 335 etc acc ate gcc CCC act tet gca ttg etc get tta gta ctg tet ttt 1353 Leu Thr lie Ala Pro Thr Ser Ala Leu Leu Ala Leu Val Leu Ser Phe 340 345 350 gcc tta tta ttg ett gcc aga gaa tta cat tgg ega cat tat ege age 1401 Ala Leu Leu Leu Leu Ala Arg Glu Leu His Trp Arg His Tyr Arg Ser 355 360 365 tta tcc cat gtg att tta aat ate ggt geg acc att tta gcc att cca 1449 Leu Ser His Val lie Leu Asn lie Gly Ala Thr lie Leu Ala He Pro 370 375 380 aeg tta gtg tta get att ggt tta ttc att tta tta cgt gag ate gat 1497 Thr Leu Val Leu Ala lie Gly Leu Phe lie Leu Leu Arg Glu lie Asp 385 390 395 400 ttt tet cca tac cat ett ttt ggg gtt gtg gta tgc Cys tgt aac geg tta 1545 Phe Ser Pro Tyr His Leu Phe Gly Val Val Val Cys Asn Ala Leu 405 410 415 get get atg cct ttt gtg ttg cgt att ttg get tta ccg atg cat aac 1593 Ala Ala Met Pro Phe Val Leu Arg lie Leu Ala Leu Pro Met His Asn 420 425 430 aat atg att tat tat gaa aaa tta tgc caa tea ett aac ctg cgt ggt 1641 Asn Met lie Tyr Tyr Glu Lys Leu Cys Gin Ser Leu Asn Leu Arg Giy 435 440 445 tgg caa cgt ttt ega ttg att gaa tgg cac aag ett cgt geg cca atg 1689 Trp Gin Arg Phe Arg Leu lie Glu Trp His Lys Leu Arg Ala Pro Met 450 455 460 aaa tac gcc ttt gca ctg get tgt geg tta tea cta ggc gat ttc acc 1737 Lys Tyr Ala Phe Ala Leu Ala Cys Ala Leu Ser Leu Gly Asp Phe Thr 465 470 475 480 gca ate geg tta ttt ggt cag get gac ttc aca teg tta ccg cat ttg 178S Ala lie Ala Leu Phe Gly Gin Ala Asp Phe Thr Ser Leu Pro His Leu 485 490 495 ttg tat caa caa ttg ggg cat tac cgt agt cag gaa geg gca gta aca 1833 Leu Tyr Gin Gin Leu Gly His Tyr Arg Ser Gin Glu Ala Ala Val Thr 500 505 510 136852-序列表.doc -143 - 1334884 gcg etc acc tta ttg gtt etc cgt ttg agt gtt ttt atg att att gaa 1881

Ala Phe lie Leu Leu Val Phe Cys Leu Ser Val Phe Met lie lie Glu S ±S ^20 525 ega cat cag gaa ccg cgt gat gat taatttaaac ggtgttcagt tttcctataa 1935

Arg His Gin Glu Pro Arg Asp Asp 530 535 tacctttact tttgageegg atttgeagat tcctgctcaa caaaaagttg ctattattgg 1995 cgccagtggc gcagggaaga gtaccttatt a-.atttgatt gcgggttttg cattgccaca 2055 gcaaggggaa atttggttga atggtgaaaa tcatagccaa actcaacctt atgaacgtcc 2115 ggtatetatt ttgtttcaag aaaacaactt gtttacgcat ttgactgtgg cagagaatat 2175 ggcattaggg ctgaaaccaa gcctaaaact gaccgcactt gaacaacaac gcgtacaaca 2235 agtggcaagt gcagtgggtt tgcagggttt tcttaatcaa ttacccaccc agttatcggg 2295 tgggcaaaaa cagcgtgtgg cgttggcgcg ttgtttatta egegataage caattttgtt 2355 attggatgaa cctttttctg ccttagatcc egatttaegg gcagaaatgt tgcatttatt 2415 gttacagttg tgtgatgaaa aaaaattaac actcctgatc gtgacacatc aagtgaacga 2475 attacagcag aaaatggatc gtatgatteg ttttgaacat ggtaggatga gtgagtccac 2535 cattttgaag gataatttta acgaaaaaca gaccgcactt taggeggett attaattaga 2595 taaggaaaga gaaaaatatg acaacggcaa cggcatttga gattcgtaca cttactcctc 2655 acccgacctt agaatattgg tctgtgtgta aaattgaage cctgtttgag accccttttt 2715 tagatttggt ttatcgggct gcgcaagtgc accgagaaaa ctttaatcct aaagccattc 2775 aattatccac tttaatgtcg attaaaacgg ggggatgtcc agaggattgt ggctactgtc 2835 cgcagtcagc acgttatcat actggagtag aaaagcagca attactcgat gtggaagaaa 2895 ttgttgaaaa agccaaaatt gccaaagcac gtggtgcagg gcgcttttgt atgggggctg 2955 catggcgtgg accgaaaccg aaagacattg aaaaagttac cgcaatcatt aaagcggtga 3015 aagaactggg cttagaaacc tgtggtacct ttggtttatt gcaagatggg atggeagaag 3075 atttaaaaga agcgggtttg gattattata accataatct cgatacagcc ccagaacact 3135 acggtaatgt gattggtacc cgtcaatttg atgategtat taatacgtta ggtaaagtgc 3195 gtaaagctgg cttaaaagtg tgctgtggcg ggattattgg catgaatgaa acccgtaaag 3255 aaagageagg attaattget agettageta atttagaccc gcaacccgaa tcggtgccga 3315 Ctaatcaatt agtgaaagtg gaaggtaccc etttageega tgcggcagaa ttagactgga 3375 cagaatttgt gcgcaccatt gcggtggcgc gtattaccat gccgaaaagc tatgtacgtt 3435 tatcagcagg gegtcaagge atgteggaag aaatgcaagc catgtgcttt atggctggcg 3495 egaatt 3501 -144 - 136852-序列表.doc

L SI 1334884 <210> 71 <211> 536 <212>蛋白質 <213>牧血性巴斯德桿菌 <400> 71

Met Phe Lys Arg Phe Arg Ala Phe Thr Tyr Arg Pro Ala Ser Tyr Leu 15 10 15

Gly Gly Met Leu Val lie Val Phe Leu Ser Ala Phe Tyr Ala Phe Ala 20 25 * 30

Leu Gly Ala Val Phe Ser Leu Pro Phe Ala Arg Ser Trp Thr Ala Leu 35 40 45

Leu Ser Asp Gin Tyr Leu Gin His Val lie lie Phe Ser Phe Trp Gin 50 55 60

Ala Phe Leu Ser Ala Val Leu Ala Val Leu Phe Gly Gly He Val Ala 65 70 75 80

Arg Ala Phe Phe Tyr Gin Pro Phe Val Gly Lys Lys Leu lie Leu Lys 85 90 95

Leu Phe Ser Leu Thr Phe Val Leu Pro Ala Leu Val Ala lie Phe Gly 100 105 110

Leu Leu Gly Val Tyr Gly Ala Ser Gly Trp Leu Ala Met Leu Ser Gin 115 120 125

Phe Phe Ala Trp Asp Trp Thr Pro Asn He Tyr Gly Leu Thr Gly lie 130 135 140

Leu Leu Ala His Leu Phe Phe Asn Val Pro Leu Ala Cys Arg Leu Phe 145 150 155 160

Leu Gin Gly Leu Gin Ala lie Pro Val Gin Gin Arg Gin Leu Ala Ala 165 170 175

Gin Leu Asn Leu Arg Gly Trp His Phe lie Arg Leu lie Glu Trp Pro 180 185 190

Tyr Leu Arg Gin Gin Leu Leu Pro Ala Phe Thr Leu lie Phe Met Leu 195 200 205

Cys Phe Thr Ser Phe Ala lie Val Leu Thr Leu Gly Gly Gly Pro Lys 210 215 220

Tyr Thr Thr Leu Glu Val Ala lie Tyr Gin Ala lie Leu Phe Glu Phe 225 230 235 240

Asp Val Pro Lys Ala Gly Leu Phe Ala Leu Leu Gin Phe Val Phe Cys 245 250 255

Phe Leu Leu Phe Thr Leu Ser Ser Phe Phe Ser Pro Ala Pro Ala Thr 260 265 270

Thr Leu His Ser Gin Pro Thr Trp Phe Ala Pro Gin Ser Tyr Trp Val 275 280 285

Lys Leu Trp Gin Arg Met lie lie Val Cys Ala Thr Val Phe lie Leu 290 295 300 -145- 136852-序列表.doc 1334884

Leu Pro Leu Leu Asn Thr Leu Val Ser Ala Leu Leu Ser Ser Gin Phe 305 310 315 320

Phe Thr Leu Trp Leu Gin Pro Gin Leu Trp Lys Ala Leu Gly Tyr Ser 325 330 335

Leu Thr lie Ala Pro Thr Ser Ala Leu Leu Ala Leu Val Leu Ser Phe 340 345 350

Ala Leu Leu Leu Leu Ala Arg Glu Leu His Trp Arg His Tyr Arg Ser 355 360 365

Leu Ser His Val lie Leu Asn lie Gly Ala Thr lie Leu Ala lie Pro 370 37S 380

Thr Leu Val Leu Ala lie Gly Leu Phe lie Leu Leu Arg Glu lie Asp 385 390 395 400

Phe Ser Pro Tyr His Leu Phe Gly Val Val Val Cys Cys Asn Ala Leu 405 410 415

Ala Ala Met Pro Phe Val Leu Arg lie Leu Ala Leu Pro Met His Asn 420 425 430

Asn Met lie Tyr Tyr Glu Lys Leu Cys Gin Ser Leu Asn Leu Arg Gly 435 440 445

Trp Gin Arg Phe Arg Leu lie Glu Trp His Lys Leu Arg Ala Pro Met 450 455 460

Lys Tyr Ala Phe Ala Leu Ala Cys Ala Leu Ser Leu Gly Asp Phe Thr 465 470 475 480

Ala lie Ala Leu Phe Gly Gin Ala Asp Phe Thr Ser Leu Pro His Leu 485 490 495

Leu Tyr Gin Gin Leu Gly His Tyr Arg Ser Gin Glu Ala Ala Val Thr 500 505 510

Ala Phe lie Leu Leu Val Phe Cys Leu Ser Val Phe Met lie lie Glu 515 520 525

Arg His Gin Glu Pro Arg Asp Asp

530 535

<210> 72 <211> 3182 <212> DNA <213>敗血性巴斯德桿菌 <220>

<221> CDS <222> (1544)..(2809) <220> <223> ygiK <220> <221〉misc 特微 <222> 452 '

<223> Ν=Λ或T 或G 或C •146· 136852·序列表.doc IS] 1334884 <4U0> 72 aactactaag gagttatgta tgaatattag aaaatcatta ctattaatat ccctagcaag 60 cttcatgtca ctttcagttt cagctgcaga aatcaatttg aaatttgaaa gttcgaattt 120 tgcaggagaa aaagtttatg aaatccaaaa agaatggact gacaatattg aaaaagcttc 180 caatgggaga ataagtatag agttattacc tctcgactca gtcttaaaat ctagtgacat 240 gctttctggt gttcgaaata aaattattga tggagcggtt gcaacagcgg caatgtatgc 300 aggcactgac cctggattcg gattaattgg tgatactatt tctgcttgga accatgacga 360

agatatttta aatttttact ataatggagg tggttttgaa gttgttgata atattttcca 420 acaatatggt gccaaaccca ttggtgtatc anttacggga gcagaatcat taccatcgaa 480 agtaaaaata gctaatactg aagattttaa aggtataaaa attcgggctc cctctggtcc 540 tatccaaaaa ttgtttgcaa gattaggagc cgctcctgtt ggtcttcctg gttcagaaat 600 ctatactagt ttagaaaaag gtattattga tgctgccgat ttctcaacgt ttgcaaataa 660 tcaagcacaa ggagtccatg atattgcaaa atatccaatc tatccgggaa ttcattcttc 720 accagccgtt catatgatta tgaatcataa aacttggagt agcttaactc catcggatca 780 agcattctta attgcttact ttaaagggat ggctctcgat actctgactc gtgctcatta 640 tgaagataaa ctagcatata aagaagcact tgagaaagga gtacaaccag tttcttggaa 900 tcaacaagaa attacaaaag ttcgttctat cgctaaagaa atttggcaag agatagctca 960 acaatctgaa ataggtaatc aagtattatc aagtattaat gaaattccta gaatctcaag 1020 gaatgctgca ataatcgtca agatggataa gacgatatcg tcttatccat taaggagtaa 1080 aacatgctta tttctaaata tttattatgg ctctgtaata agctagatca aatattcatt 1140 aaagtaggtt attacgtttc ttatattttt ctattagttg ttatcattgg ttttacgagg 1200 ttgttgctcg gtatttattc tctagcccaa cactttgggt tcatgaagta acaacatttt 1260 taataagtct atcattactt tatggtggag tagcttgtta cgccagtaat aaacatattg 1320 ccatgacatt tatcagacaa aaattaccta ataagatcaa atggttacta gaactcttag 1380 ttgaaatact tacttttatc ttctttattt tgcttagtta cggagcatac ttatcagcta 1440 gagaagcatt atttactcca tcaggaaaat tcaaaatgca aacttctgga agtgtattag 1500 acatgccatt tccagcaatt gaaaaaagtt tcttctttat ttc atg cct cat cat 1555

Met Pro His His tgt tgt tct ttc Cys Cys Ser Phe 5 tea gga gga att Ser Gly Gly lie ccg tea cat eta Pro Ser His Leu 15 ttt ggg ata ggt Phe Gly lie Gly 30 agt act aca tat att Ser Thr Thr Tyr lie 10 ate atg ata agt gca lie Met lie Ser Ala 25 tac aaa ata 1603 Tyr Lys lie 20 att gga act 1651 lie Gly Thr 35 •147· U6852-序列表.doc is] 1334884 ctt att ate ttt tta atg atg att tee ett tta ttt att gga atg cca 1699

Leu He lie Phe Leu Met Met He Ser Leu Leu Phe He Gly Met Pro 4 U 4 5 50 tta ggt ttt etc act ggg tta ate get tta gtt att tea tat ctt tgg 1747

Leu Gly Phe Leu Thr Gly Leu lie Ala Leu Val .lie Ser Tyr Leu Trp 55 60 65 ttt gat act acc gca ata atg caa atg ata get tea cgt gtc act gat 1795

Phe Asp Thr Thr Ala He Met Gin Met He Ala Ser Arg Val Thr Asp 70 75 80 ttc aca tea tet tac act ttt gta get gtg cct atg ttt gtt ctt atg 1843

Phe Thr Ser Ser Tyr Thr Phe Val Ala Val Pro Met Phe Val Leu Met @5 90 95 100 gca aca tta ctt gat aag act gga ,att get aga gat etc tac aac gca 1891

Ala Thr Leu Leu Asp Lys Thr Gly He Ala Arg Asp Leu Tyr Asn Ala 105 110 115 atg ega gtc att ggc ggt ega tta ega ggt gga att gca att caa teg 1939

Met Arg Val He Gly Gly Arg Leu Arg Gly Gly lie Ala lie Gin Ser

120 125 130 atg ttt gtt gca gtt eta ctt get aeg atg tea ggt att ate ggt gga 1987

Met Phe Val Ala Val Leu Leu Ala Thr Met Ser Gly He He Gly Gly 135 140 145 gaa act gtt tta tta ggc atg ttg gca tta cca caa atg tta ege tta 2035

Glu Thr Val Leu Leu Gly Met Leu Ala Leu Pro Gin Met Leu Arg Leu 150 155 160 ggc tat aat aaa aac tta get ata gga act gtt gta gca gga gga gca 2083

Gly Tyr Asn Lys Asn Leu Ala lie Gly Thr Val Val Ala Gly Gly Ala 165 170 175 180 ttg ggt aca atg gtt cct cca agt ate gtg ttg att att tac gga atg 2131

Leu Gly Thr Met Val Pro Pro Ser lie Val Leu lie lie Tyr Gly Met 185 190 195 acc gca aat gtt tet att gga gaa eta ttt ctt gca gca att cca gcc 2179

Thr Ala Asn Val Ser lie Gly Glu Leu Phe Leu Ala Ala He Pro Ala 200 205 210

tcc tta eta ctt tet aca ttc tat att eta tat att eta gta ctt tgc 2227

Ser Leu Leu Leu Ser Thr Phe Tyr lie Leu Tyr lie Leu Val Leu Cys 215 220 225 tac ttc aaa cct age tat ggc cct gca atg cct age tea gaa aat cat 2275

Tyr Phe Lys Pro Ser Tyr Gly Pro Ala Met Pro Ser Ser Glu Asn His 230 235 240 aca tta aeg aaa gaa gat att aaa aaa att att cat gat att gca att 2323

Thr Leu Thr Lys Glu Asp lie Lys Lys lie lie His Asp lie Ala lie 245 250 255 260 cca gta get ate gcc aca tgg att tta gga agt att tat ggc ggg ata 2371

Pro Val Ala lie Ala Thr Trp lie Leu Gly Ser lie Tyr Gly Gly lie 265 27〇 275 gca tea ate act gaa tet gcc tgt gtt ggt gta gtt ggg gta ata tta 2419

Ala Ser lie Thr Glu Ser Ala Cys Val Gly Val Val Gly Val lie Leu 280 285 290 •148· 136852-序列表.doc tsi 1334884 gca gca ttc tat cga aaa 9aa tta aat ttc aaa ata gta caa gaa tea 2467

Ala Ala Phe Tyr Ara Lys Glu Leu Asn Phe Lys lie Val Gin Glu Ser 295 ** 300 305 eta aaa cat aca ate aat act gtt ggt atg ata ate tgg gtc ggc att 2515

Leu Lys His Thr lie Asn Thr Val Glv Met He lie Trp Val Gly lie 310 315 320 ggc gca aca atg att ata ggt att tat aat eta atg ggt ggg gac cga 2563

Gly Ala Thr Met lie lie Gly lie Tyr Asn Leu Met Gly Gly Asp Arg 325 330 335 340 ttt ata get aac tta ttc get age tta gat gcc tet cca att tat act 2611

Phe lie Ala Asn Leu Phe Ala Ser Leu Asp Ala Ser Pro lie Tyr Thr 345 350 355 ate att att atg atg gtt att tta tta ata ett ggt atg ttc tta gat 2659 lie lie lie Met Met Val lie Leu Leu lie Leu Gly Met Phe Leu Asp 360 365 370 tgg att ggt gtt gcc atg ttg act ttc etc aag aca agt aaa geg aca 2707

Trp lie Gly Val Ala Met Leu Thr Phe Leu Lys Thr Ser Lys Ala Thr 375 380 385 ate aat ttg tgt ttt gac ata gtc agg tac agt att tgg cgt ggt ccc 2755 lie Asn Leu Cys Phe Asp lie Val Arg Tyr Ser lie Trp Arg Gly Pro 390 395 400 tcc ttc cac agt acc aat gtt cat cgt ggt acc ttt gtc ggg ege ggt 2803

Ser Phe His Ser Thr Asn Val His Arg Gly Thr. Phe Val Gly Arg Gly 405 410 415 420 act ttt tagtaaatet tgcgcgatac gaataaacgc attgatggea tttgctccgt 2859 Thr Phe tttgtggatc gactgccgca tgageagatt tgccaaaaaa ttcaattaca tacttcccad 2919 tcccttttct ttcgttaacg tttccactta gattgcccag aageegatet gtctgaatgg 2979 gaacaagtgt tataccaaga agcgaatcca acaggtgaag tggtgatcgg tatggtgggt 3039 aaatacactg aattaccgga tgcctacaaa tcggttaatg aagccttgaa acacgcaggc 3099

ttaaaaaacc gtcttagcgt gcaaatcaaa tatattgatt cacaagatgt ggaaaccaaa 3159 ggcacagaag tgttagaagg cgt 3182 <210> 73 <211> 422 <212>蛋白質 <213>改血性巴斯德桿菌 <400> 73

Met Pro His His Cys Cys Ser Phe Ser Thr Thr Tyr lie Pro Ser His 1 5 10 15

Leu Tyr Lys He Ser Gly Gly He lie Met He Ser Ala Phe Gly lie 20 25 30

Gly He Gly Thr Leu He lie Phe Leu Met Met He Ser Leu Leu Phe 35 40 45 -149· 136852* 序列表.doc i si 1334884 lie Gly Met Pro Leu Gly Phe Leu Thr Gly Leu lie Ala Leu Val lie 50 55 60

Ser Tyr Leu Trp Phe Asp Thr Thr Ala lie Met Gin Met lie Ala Ser 65 70 75 80

Arg Val Thr Asp Phe Thr Ser Ser Tyr Thr Phe Val Ala Val Pro Met 8¾ 90 95

Phe Val Leu Met Ala Thr Leu Leu Asp Lys Thr Gly lie Ala Arg Asp 100 105 110

Leu Tyr Asn Ala Met Arg Val lie Gly Gly Arg Leu Arg Gly Gly lie 115 120 125

Ala lie Gin Ser Met Phe Val Ala Val Leu Leu Ala Thr Met Ser Gly 130 135 140 lie lie Gly Gly Glu Thr Val Leu Leu Gly Met Leu Ala Leu Pro Gin 145 150 155 160

Met Leu Arg Leu Gly Tyr Asn Lys Asn Leu Ala lie Gly Thr Val Val 165 170 175

Ala Gly Gly Ala Leu Gly Thr Met Val Pro Pro Ser lie Val Leu lie 180 185 190 lie Tyr Gly Met Thr Ala Asn Val Ser lie Gly Glu Leu Phe Leu Ala 195 200 205

Ala lie Pro Ala Ser Leu Leu Leu Ser Thr Phe Tyr He Leu Tyr lie 210 215 220

Leu Val Leu Cys Tyr Phe Lys Pro Ser Tyr Gly Pro Ala Met Pro Ser 225 230 235 240

Ser Glu Asn His Thr Leu Thr Lys Glu Asp lie Lys Lys lie lie His 245 250 255

Asp lie Ala lie Pro Val Ala lie Ala Thr Trp lie Leu Gly Ser lie 260 265 270

Tyr Gly Gly lie Ala Ser lie Thr Glu Ser Ala Cys Val Gly Val Val 275 280 285

Gly Val lie Leu Ala Ala Phe Tyr Arg Lys Glu Leu Asn Phe Lys lie 290 295 300

Val Gin Glu Ser Leu Lys His Thr lie Asn Thr Val Gly Met lie He 305 310 315 320

Trp Val Gly lie Gly Ala Thr Met lie lie Gly lie Tyr Asn Leu Met 325 330 335

Gly Gly Asp Arg Phe lie Ala Asn Leu Phe Ala Ser Leu Asp Ala Ser 340 345 350

Pro lie Tyr Thr lie lie lie Met Met Val lie Leu Leu lie Leu Gly 355 360 365

Met Phe Leu Asp Trp lie Gly Val Ala Met Leu Thr Phe Leu Lys Thr 370 375 380 -150- 136852·序列表.doc 1334884 ^ei uys irn： l丄e hsii ueu cys ^ne Asp iie Va丄 /urg ryr tier lie 385 390 395 400

Trp Arg Gly Pro Ser Phe His Ser Thr Asn Val His Arg Gly Thr Phe 405 4X0 415

Val Gly Arg Gly Thr Phe 420

<210> 74 <211> 2787 <212> DNA <213>收血性巴斯谂桴菌 <220>

<221> CDS <222> (463)..(936) <220>

<223> yhcJ <400> 74 gttaacacac catgattaat gatgccggtt gaagccactg caacgtaatc gaattgtccg 60 gcatacaaag caagaatgtt ggccagtgtg tcatgcatcg cattggcagc atcagcttgt 120 ggcgttgcaa tctgttggcg ttgttctatt ttgccgtctg ttacaatagc cgaggcaatt 180 tttgttccac caatatctaa tgctaaacag cgcataggct ctccttctgt gatgacttat 240 tttgccgatt tgacggcatc ggcaaaccag cttacgatat gttcgaggcg agtcagcgca 300 gatcctacgg tgacagagta agcaccaatc tcaattgcgg ttttcgccaa ttctggggtg 360 ttatagcgcc cttctgccat cactcggcag ccagcagcat tcaaatcttt gactaactga 420 taatccggtt cagctggaat ttcaccgcca gtataaccag ac atg gtg eta cca 474

Met Val Leu Pro ata att tet acc cct aag ttg tgg caa tac ate cct tet tea aaa tta 522 lie lie Ser Thr Pro Lys Leu Trp Gin Tyr lie Pro Ser Ser Lys Leu 5 10 15 20

gaa caa tcc gcc atg get aaa caa cct aat tet ttg att cgt tta ata 570

Glu Gin Ser Ala Met Ala Lys Gin Pro Asn Ser Leu lie Arg Leu lie 25 30 35 atg get tea cgt gta gtt gga egg aeg ega teg gta cca tea aaa gca 618

Met Ala Ser Arg Val Val Gly Arg Thr Arg Ser Val Pro Ser Lys Ala 40 45 50 ata ata teg geg cct get geg get aac tet tea atg tet tgt aaa aat 666 lie lie Ser Ala Pro Ala Ala Ala Asn Ser Ser Met Ser Cys Lys Asn 55 60 65 ggg eta ata ega aeg gga ctg tea ggt aaa teg cgt tta aeg ata cca 714

Gly Leu lie Arg Thr Gly Leu Ser Gly Lys Ser Arg Leu Thr lie Pro 70 75 80 ata ate ggt aca ctg aeg aeg tta ege gtg get ttt aaa ttt teg ate 762 lie lie Gly Thr Leu Thr Thr Leu Arg Val Ala Phe Lys Phe Ser lie • 151 · 136852-序列表.doc

l SI 1334884 tib 90 95 100 ccc tea aca cgt aac ccg gca gca cca ccg ata aeg gat get tgc gee 810

Fro Ser lie Arg Asn Pro Ala Ala Pro Pro lie Thr Asp Ala Cys Ala 105 110 115 aeg. geg gca aca att tet ggc gag tee att ggc cca tta tet aeg ggc 858

Met Ala Ala Thr lie Ser Gly Glu Ser lie Gly Pro Leu Ser Thr Gly 120 125 130 tgg caa gat geg att aag cca tat tt i att tgt tet aaa act tgc gga 906

Trp Gin Asp Ala lie Lys Pro Tyr Leu lie Cys Ser Lys Thr Cys Gly 135 140 145 tgt gat agt ttt gac ata tta act cca gtc taaatttatc aaaagaagat 956

Cys Asp Ser Phe Asp lie Leu Thr Pro Val 150 155

tgactccaat tttgagatet ttgtcaatcc ttgageaett agttcaccgt caatgttgtt tgattgaccg tgagtcagaa caaatatcat ggtttaacga acggtttgtc gaatgaccaa cgttgataaa agtattatat attcaatttt tagttttttc atcccaacgt ctttatgttt tggttgcggg aagaaaaatc accgtgtgat cacgcttcca atcctgaaac ttgcataggt ttgtcgcata ttgctatttt gataaccttg tagaatcaca ttaaataggt gtgtggcttc tgtcatttaa tgacgcctaa taacatccaa ccgctaattg ttaagctgat tatcctgatc aaaacctgat gtgacgaacc actttagaat aaccaataga a9Qtgtttca tccaagctca caatggcaaa gattaaacaa aatttacttc ctccaaaaaa taatettaga tttattcata ttgtgtttgc ctcattaaaa aaagtatttt attgagcctg taataaaaag ctatcgcatg aaaaagaaaa atattgtege aggtgtgcct attggcaggt aettageatg atgccggtaa tatcatttat gccaaacgtg ggagaactca gcttctgtat aaegaatata attgatgtgg ttaaaagacg ccagaagcag gcgttgcccg attaaaaaat gggaatagac tggtttgcga tgtaatccgt tgtgtttcaa tgaatttgtg tagtgggacg acttgcgccg acagattgtc gtactgacgc gctacctgtt ttggaaaatt tgtggacctc acagtaaact gaaataaaac tgaaatttat taatgaaatt ttgeageaga aageagtaga ctattttccc gtgeattaga aagtatttgc acacaaaatt aacaaccaaa agettaattt tacactgttc cggtacaaag egtaagteae cgttagtgac taggagaaat gtgaatacgt caagttgttg cagaaagtcc cgtcccacat ccgccatttt tattttgaaa tatcttccgt ttcattttct ttcatcatca taaaaaacta ttaegatett attctttgcg tagctcacag ctttacgtta gttgeettat tggtcaaggt ataataaaaa 1016 tagttatgat 1076 taatattget 1136 gcgtaaatcc 1196 gtctgacgga 1256 egtattaate 1316 ggtgtgtagg 1376 ttcttcctta 1436 aatccgttta 1496 taaattggcg 1556 gtcaaaaagt 1616 getetgatag 1676 taaaacgcta 1736 aggggtaaat 1796 atataaaaaa 1856 ttttaacgta 1916 etaettgeat 1976 aaattcggta 2036 aaagaagtga 2096 ttaggtgatg 2156 ggtgaatcag 2216 atgattccta 2276 ttattqaaaa 2336 •152- 136852·序列表.doc i si 1334884 aaactgacaa agagttaaac gcacaagtgt catctgtggc gtataacggt acacgtcaaa 2396 caacccctaa ccgtgcaacc aacagcattg aagacatgaa agggttaaaa ttacgtgtac 2456 ctaacgcggc aaccaacctt gcttatgcaa aatacgtggg tgcagcgcca acaccaatgg 2516 cattctctga agtttacctt gcgcttcaaa caaactctgt ggatggtcaa gaaaacccat 2576 caccgacaac ccaagcacaa aaactctatg aagtacaaaa atacttagcg ttaactaacc 2636 acatc**taaa tgaccaactt tacttaatca gtaacgatac gt.tggcagat ttaccagaag 2696 atttacaaaa agtggttaaa gatgcagcag cgaaagccgc tgaatatcac actaaactct 2756 tcgttgacgg tgagaacagc ttagttgaat t 2787 <210> 75 <211> 158 <212>蛋白質 <213>敗血性巴斯德桿菌

<400> 75

Met Val Leu Pro lie lie Ser Thr Pro Lys Leu Trp Gin Tyr lie Pro 15 10 15

Ser Ser Lys Leu Glu Gin Ser Ala Met Ala Lys Gin Pro Asn Ser Leu 20 25 30 lie Arg Leu lie Met Ala Ser Arg Val Val Gly Arg Thr Arg Ser Val 35 40 45

Pro Ser Lys Ala lie lie Ser Ala Pro Ala Ala Ala Asn Ser Ser Met 50 55 60

Ser Cys Lys Asn Gly Leu lie Arg Thr Gly Leu Ser Gly Lys Ser Arg 65 70 75 80

Leu Thr lie Pro lie lie Gly Thr Leu Thr Thr Leu Arg Val Ala Phe 85 90 95

Lys Phe Ser lie Pro Ser lie Arg Asn Pro Ala Ala Pro Pro He Thr 100 105 110

Asp Ala Cys Ala Met Ala Ala Thr He Ser Gly Glu Ser He Gly Pro 115 120 125

Leu Ser Thr Gly Trp Gin Asp Ala He Lys Pro Tyr Leu lie Cys Ser 130 135 140

Lys Thr Cys Gly Cys Asp Ser Phe Asp lie Leu Thr Pro Val 145 150 155

<210> 76 <211> 2787 <212> DNA <213>敗血性巴斯德桿菌 <220>

<221> CDS <222> (1949) . . (2785) •153- 136852·序歹〇表.doc 1S1 1334884 <220>

<223> viaO <400> 76 gttaacacac catgattaat gatgccggtt gaagccactg caacgtaatc gaattgtccg 60 gcatacaaag caagaatgtt ggccagtgtg tcatgcatcg cattggcagc atcagcttgt 120 ggcgttgcaa tctgttggcg ttgttctatt ttgccgtctg ttacaatagc cgaggcaatt 180 tttgttccac caatatctaa tgctaaacag cgcataggct ctccttctgt gatgacttat 240 tttgccgatt tgacggcatc ggcaaaccag cttacgatat gttcgaggcg agtcagcgca 300 gatcctacgg tgacagagta agcaccaatc tcaattgcgg ttttcgccaa ttctggggtg 360 ttatagcgcc cttctgccat cactcggcag ccagcagcat tcaaatcttt gactaactga 420 taatccggtt cagctggaat ttcaccgcca gtataaccag acatggtgct accaataatt 480 tctaccccta agttgtggca atacatccct tcttcaaaat tagaacaatc cgccatggct 540

aaacaaccta attctttgat tcgtttaata atggcttcac gtgtagttgg acggacgcga 600 tcggtaccat caaaagcaat aatatcggcg cctgctgcgg ctaactcttc aatgtcttgt 660 aaaaatgggc taatacgaac gggactgtca ggtaaatcgc gtttaacgat accaataatc 720 ggtacattga cgacgttacg cgtggctttt aaattttcga tcccttcaat acgtaacccg 780 gcagcaccac cgataacgga tgcttgcgcc atggcggcaa caatttctgg cgagtccatt 840 ggcccattat ctacgggctg gcaagatgcg attaagccat atttaatttg ttctaaaact 900 tgcggatgtg atagttttga catattaact ccagtctaaa tttatcaaaa gaagattgac 960 tccaatttgc ataggttaat cttagaatta aaaaataaca accaaaataa taaaaatttg 1020 agatctttgt cgcatattta ttcataggga atagacagct taattttagt tatgatttgt 1080 caatccttgc tattttttgt gtttgctggt ttgcgataca ctgttctaat attgctttga 1140 gcacttgata accttgctca ttaaaatgta atccgtcggt acaaaggcgt aaatccagtt 1200

caccgttaga atcacaaaag tatttttgtg tttcaacgta agtcacgtct gacggacaat 1260 gttgttttaa ataggtattg agcctgtgaa tttgtgcgtt agtgaccgta ttaatctgat 1320 tgaccggtgt ggcttctaat aaaaagtagt gggacgtagg agaaatggtg tgtaggtgag 13Θ0 tcagaatgtc atttaactat cgcatgactt gcgccggtga atacgtttct tccttacaaa 1440 tatcattgac gcctaaaaaa agaaaaacag attgtccaag ttgttgaatc cgtttaggtt 1500 taacgataac atccaaatat tgtcgcgtac tgacgccaga aagtcctaaa ttggcgacgg 1560 tttgtcccgc taattgaggt gtgcctgcta cctgttcgtc ccacatgtca aaaagtgaat 1620 gaccaattaa gctgatattg gcaggtttgg aaaattccgc cattttgctc tgatagcgtt 1680 gataaatatc ctgatcactt agcatgtgtg gacctctatt ttgaaataaa acgctaagta 1740 ttatataaaa cctqatatqc cqqtaaacag taaacttatc ttccqtaaag qtaaatattc 1800 -154- 136852·序列表.doc 1334884 aattttgtga cgaacctatc atttatgaaa taaaacttca ttttctatat aaaaaatagt i860 tttttcactt tagaatgcca aacgtgtgaa atttatttca tcatcatttt aacgtaatcc 1920 caacgtaacc aatagaggag aactcata atg aaa ttt aaa aaa eta eta ett 1972 Met Lys Phe Lys Lys Leu Leu Leu 1 5 gca tct tta tgt tta ggt gtt tea get tct gta ttt gca gca gat tac Ala Ser Leu Cys Leu Gly Val Ser Ala Ser Val Phe Ala Ala Asp Tyr 10 15 20 2020 gat ett aaa ttc ggt atg gtt gcg ggt cca age tea aac gaa tat aaa 2066 Asp Leu Lys Phe Gly Met Val Ala Gly Pro Ser Ser Asn Glu Tyr Lys 25 30 35 40 gca gta gaa ttc ttt gcg aaa gaa gtg aaa gaa aaa tcc aat ggc aaa 2116 Ala Val Glu Phe Phe Ala Lys Glu Val Lys Glu Lys Ser Asn Gly Lys 45 50 55 att gat gtg get att ttc cct age tea cag tta ggt gat gac cgt gtg 2X64 lie Asp Val Ala lie Phe Pro Ser Ser Gin Leu Gly Asp Asp Arg Val 60 65 70 atg att aaa caa tta aaa gac ggt gca tta gac ttt aeg tta ggt gaa 2212 Met lie Lys Gin Leu Lys Asp Gly Ala Leu Asp Phe Thr Leu Gly Glu 75 80 85 tea gca cgt ttc caa att tac ttc cca gaa gca gaa gta ttt gcg ttg 2260 Ser Ala Arg Phe Gin lie Tyr Phe Pro Glu Ala Glu Val Phe Ala hen 90 95 100 cct tat atg att cct aat ttt gaa acc tct aaa aaa gcg ttg etc gac 2308 Pro Tyr Met lie Pro Asn Phe Glu Thr Ser Lys Lys Ala Leu Leu Asp 105 110 115 120 aca aaa ttt ggt caa ggt tta ttg aaa aaa att gat aaa gag tta aac 2356 Thr Lys Phe Gly Gin Gly Leu Leu Lys Lys He Asp Lys Glu Leu Asn 125 130 135 gta caa gtg tta tct gtg gcg tac aac ggt aca cgt caa aca act tct 2404 Val Gin Val Leu Ser Val Ala Tyr Asn Gly Thr Arg Gin Thr Thr Ser 140 145 150 aac cgt gca ate aac age att gaa gac atg aaa ggg tta aaa tta cgt 2452 Asn Arg Ala lie Asn Ser lie Glu Asp Met Lys Gly Leu Lys Leu Arg 155 160 165 gta cct aac gcg gca acc aac ett get tat gca aaa tac gtg ggt gca 2500 Val Pro Asn Ala Ala Thr Asn Leu Ala Tyr Ala Lys Tyr Val Gly Ala 170 175 180 gcg cca aca cca atg gca ttc tct gaa gtt tac ett gcg ett caa aca 2548 Ala Pro Thr Pro Met Ala Phe Ser Glu Val Tyr Leu Ala Leu Gin Thr 185 190 195 200 aac tct gtg gat ggt caa gaa aac cca tta ccg aca Asn Ser Val Asp Gly Gin Glu Asn Pro Leu Pro Thr 205 210

an al c G a d 5 c 1 1 9 A 2 an al c G c e t 1 cd I 2596 aaa ttc tat gaa gta caa aaa tac tta gcg tta act aac cac ate tta 2644 Lys Phe Tvr Glu Val Gin Lvs Tyr Leu Ala Leu Thr Asn His lie Leu 220 225 230 136852·序列表.doe •155·

SI 1334884 aat gac caa ctt tac tta ate agt aac gat aeg ttg gca gat tta cca Asn Asp Gin Leu Tvr Leu lie Ser Asn Asp Thr Leu Ala Asp Leu Pro 235 240 245 gaa gat tta caa aaa gtg gtt aaa gat gca gca geg aaa gec get gaa Glu Asp Leu Gin Lys Val Val Lys Asp Ala Ala Ala Lys Ala Ala Glu 250 255 260 # tat cac act aaa etc ttc gtt gac ggt gag aac age tta gtt gaa tt Tyr His Thr Lys Leu Phe Val Asp Gly Glu Asn Ser Leu Val Glu 265 270 275 2692 2740 2787 <210> 77 <211> 279 <212>蛋白質 <213>敗血性巴斯德桴茵

<400> 77 Met Lys Phe 1 Lys Lys 5 Leu Leu Leu Ala Ser 10 Leu Cys Leu Gly Val 15 Ser Ala Ser Val Phe 20 Ala Ala Asp Tyr Asp 25 Leu Lys Phe Gly Met 30 Val Ala Gly Pro Ser 35 Ser Asn Glu Tyr Lys 40 Ala Val Glu Phe Phe 45 Ala Lys Glu Val Lys 50 Glu Lys Ser Asn Gly Lys 55 lie Asp Val Ala 60 lie Phe Pro Ser Ser 65 Gin Leu Gly Asp Asp Arg 70 Val Met lie Lys 75 Gin Leu Lys Asp Gly 80 Ala Leu Asp Phe Thr 85 Leu Gly Glu Ser Ala 90 Arg Phe Gin lie Tyr 95 Phe Pro Glu Ala Glu 100 Val Phe Ala Leu Pro 105 Tyr Met lie Pro Asn 110 Phe Glu Thr Ser Lys 115 Lys Ala Leu Leu Asp Thr 120 Lys Phe Gly Gin Gly 125 Leu Leu Lys Lys 130 lie Asp Lys Glu Leu 135 Asn Val Gin Val Leu 140 Ser Val Ala Tyr Asn 145 Gly Thr Arg Gin Thr 150 Thr Ser Asn Arg Ala 155 lie Asn Ser He Glu 160 Asp Met Lys Gly Leu 165 Lys Leu Arg Val Pro 170 Asn Ala Ala Thr Asn 175 Leu Ala Tyr Ala Lys 180 Tyr Val Gly Ala Ala 185 Pro Thr Pro Met Ala 190 Phe Ser Glu val Tyr 195 Leu Ala Leu Gin Thr 200 Asn Ser Val Asp Gly Gin 205 Glu Asn Pro Leu 210 Pro Thr lie Gin Ala 215 Gin Lys Phe Tyr Glu 220 Val Gin Lys Tyr Leu 225 Ala Leu Thr Asn His 230 lie Leu Asn Asp Gin 235 Leu Tyr Leu lie Ser 240 136852·序列表.doe •156- IS] 1334884

Asn Asp Thr Leu Ala Asp Leu Pro Glu Asp Leu Gin Lys Val Val Uys 245 250 255

Asp Ala Ala Ala Lys Ala Ala Glu Tyr His Thr Lys Leu Phe Val Asp 260 265 270

Gly Giu Asn Ser Leu Val Glu 275

<210> 78 <211> 2590 <212> DKA <213>敗血性巴斯德桿菌 <220>

<221> CDS <222> (908) . . (1294) <220>

<223> yjgF <400> 78 ctgcaggctc gattagtggg gcaccgaaag aaaaaaccgt gcaaattatt cacgccgcag 60 aacagcagcc acgcggttat tacacgggga tttttggtct gttcgatggt gagtcgttac 120 aaagtgcggt ggcaattcgt tttattgagc aagtggacga gaaattgatt ttccgcagcg 180 gtggcgggat tacgatctta agcgagctag aagacgagta ccaagaattg atccaaaaag 240 tgtatgtacc agtaggataa gcgatgacat ttcctttatt tgagacgatc gctattgtga 300 acggtgaaat tcagcacctt gccctgcatc aacaacgtta tgcggcaagt ttggcgacct 360 tttacggcga gaaaggagcg aaagtacagg atcttgcgaa aattattcag attccgaccg 420 cacttgaaca cactcaacat gcgccgataa tccgttgtcg gattgattac aatcagcaag 480 actgtgacgt gcattatttt ccctatcaac gcaaaattta ccgcactttt cagcctgtca 540 tttgcgatga aattaactat gatctgaaat atgctgatcg ggcattatta aatcagttat 600

ttgctcagcg tagggattgt gatgagatta tgattatcaa acacggcaag gtgacggatt 660 gcagtattgg taatctggtg tttcgccaag gtgagcaatg gttcacgcca gatagccc.gt 720 cattttacgg cacacaacga gcctggttat tacaacaagg caaaattcaa gcccgttcca 780 tcttattgca agagatcgca caatttgaag aaattcggtt aattaatgca ctaaatccgc 840 tgtaaatttt ccttgaacag cgtaaaataa aacaactttt tcagccagat aaaaggagat 900 aaacgac atg acg aaa gta att cat act gac aat gca cca gcc gcc att 949

Met Thr Lys Val lie His Thr Asp Asn Ala Pro Ala Ala lie 1 5 10 ggt cct tat gta caa gcg gta gat tta ggt aat atg ctg tta acc tct 997

Gly Pro Tyr Val Gin Ala Val Asp Leu Gly Asn Met Leu Leu Thr Ser 15 20 25 30 ggg caa att cca gtg aac cca aaa acc ggt gaa gtg cca gcg gat ate 1045

Gly Gin lie Pro Val Asn Pro Lys Thr Gly Glu Val Pro Ala Asp lie -157- 136852-序列表.doc i si 1334884 35 40

4S gta gca caa gca cgt caa teg tta gaa aac gtg aaa geg att gtg gaa 1093

Val Ala Gin Ala Arg Gin Ser Leu Glu Asn Val Lys Ala lie Val Glu 50 55 60 caa geg gga tta caa gtc gca aat ate gtg aaa acc aeg gtg ttt gtg 1141

Gin Ala Gly Leu Gin Val Ala Asn lie Val Lys Thr Thr Val Phe Val 65 70 75 aaa gat tta aat gac ttt gca geg gtc aat geg gag tat gaa cgt ttc 1189

Lys Asp Leu Asn Asp Phe Ala Ala Val Asn Ala Glu Tyr Glu Arg Phe 80 85 90 ttt aaa gag aac aat cac cct age ttc cct get cgt tea tgt gtg gaa 1237

Phe Lys Glu Asn Asn His Pro Ser Phe Pro Ala Arg Ser Cys Val Glu 95 100 105 110 gtg gca cgt ttg ccg aaa gat gtg ggg att gaa ate gag gca ate get 1285

Val Ala Arg Leu Pro Lys Asp Val Gly lie Glu lie Glu Ala lie Ala 115 120 125

gta aaa gee taatgaatag ettgeattta tettagtegt agcaaaacaa Val Lys Ala 1334

tctcttttca cttgctctct tcaaagcaag ttgataagtg atttttattg ggcgtttttc 1394 tattgatage caaaaacgcc ctttactgat agagaataaa ctatgcaaaa tcaagtcatc 1454 gagattctac aataccgttt aaaaccacaa tcaggacaaa cgtttcacca aattatgcgt 1514 gagatcagtg ttccactcca taaacaacat gggattgatg teattgegta tggaaattca 1574 ttacatgata ttgacagcta ttatttaatc cgtgcatttg agacagaaac caaattgcaa 1634 cagcagctcg atgcttttta tgccagtgat gattggcgtg atggaccaag agaaagtatc 1594 attcgcctga ttgaaagcag tttaaaatcg gtgatcatgc tcccgacaca ggcaatccat 1754 gcactacgca accattatcc tcaataaaat caacaaccgc acccaatcag tgcggtcatt 1814 ttttcttact ttttcagtgc taagggaaaa acaacgatag tggacgttgt ttaatcaatt 1874 tccaaacaca ttgegegata tcacaccaac tctcaatttc tgtttctaaa gaacgcagcg 1934 caacccataa egegataaag aaactgacaa tcaaattcac cataccaatc aataacacga 1994 acactaaacc ttgtaagaac atctgccaag taaacgcgcc actgatcgcc atatagccca 2054 aattcgcaga agaaaacgcc acatggcgaa tatetaaegg taaattaagc aaatacccga 2114 ctaaccccgt taaaccaagc aataaaccaa aacacagatt tcccataatc gaaccgtaat 2174 tatcatgcca gtattctgca aatttgtagc gcatattacg ggtcaacaga cggcgtaaaa 2234 tagggtgatt tettagtege atttttaagt tcaaataatt actacgatta tcaaaataac 2294 cagaaataat cccagaaaag aataaccaga aacccgcaat ggcggcaaac cataaggacc 2354 ctttcatcgg atcaagggat ttttgttggt aggcaatctc cgcgtcactc aataaaggtg 2414 taccaacata atgttgatag cctagcgcaa gcaaacaagc cacagaaatc gctaaagtga 2474 -158- 136852-序列表.doc

I SI 1334884 cattacccaa gactgccact gtttgcgagc gaaacacatc gatcagcagt tgagctaatt 2534 gcagactaac ggacttgcct tgtccattat ccaccgtttc tgcaaaacgt gctgca 2S90 <210> 79 <211> 129 <212>蛋白質 <213 >改血性巴斯德桿茵 <400> 79

Met Thr Lys Val He His Thr Asp Asn Ala Pro Ala Ala lie Gly Pro 15 10 15

Tyr Val Gin Ala Val Asp Leu Gly Asn Met Leu Leu Thr Ser Gly Gin 20 25 30 lie Pro Val Asn Pro Lys Thr Gly Glu Val Pro Ala Asp lie Val Ala 35 40 45

Gin Ala Arg Gin Ser Leu Glu Asn Val Lys Ala He Val Glu Gin Ala 50 55 60

Gly Leu Gin Val Ala Asn lie Val Lys Thr Thr Val Phe Val Lys Asp 65 70 7S 80

Leu Asn Asp Phe Ala Ala Val Asn Ala Glu Tyr Glu Arg Phe Phe Lys 85 90 95

Glu Asn Asn His Pro Ser Phe Pro Ala Arg Ser Cys Val Glu Val Ala 100 105 110

Arg Leu Pro Lys Asp Val Gly lie Glu lie Glu Ala lie Ala Val Lys 115 120 125

Ala

<210> 80 <211> 6642 <212> DNA c213>敗血性巴斯德桿菌

<220>

<221> CDS <222> (463) . . (1884) <220> <223> yleA <400> 80 aacccagctc agataagttt aattctggct atggaaagtg gaccaaatcc ttcgatctcc 60 caggtttcta aggcgcgata cgggcaaata cgagtatttt cgatatactg ccaagcacct 120 agttgtttta agagattaac ggaagcaaca ctgatcgcag aaatgcgaag atcgtaaggc 180 gaattaggtt gaaactgtgg gagtggggcg tgttcgataa gctggacttg atgtccttgt 240 ttacgtaagc caagcgcaca ggctgcacca accatgccac cgccaaccac gatcatgtct 300 ttttgtatga cgtccatagg attttccttt tctttttgtt acgtattcta ccgtcaatga 360 • 159· 136852-^lM.doc

l SI 1334884

gggaatttca aaagaaatct cttttttagc tagccagcat aggttcaaga ctgtaaaata gtcagtcaca tttttatagg ttaactgaat tttttaaacg at atg acg caa aaa

Met Thr Gin Lys tta cat att aaa acg tgg ggt tgt cag atg aat gaa tat gat tea tet

Leu His lie L-ys Thr Tm Glv Cys Gin Met Asn Glu Tyr Asp Ser Ser 5 10 15 20 aaa atg gca gat etc tta aac agt aoc cac ggc tta gag zta aca gaa Lvs Met Ala Asp Leu Leu Asn Ser Thr His Gly Leu Glu Leu Thr Glu * 25 30 35 att ccg gaa gaa geg gat gtg tta ttg tta aac act tgc tea att cgt lie Pro Glu Glu Ala Asp Val Leu Leu Leu Asn Thr Cys Ser lie Arg 40 45 50 gaa aaa gca caa gaa aaa gtt ttc cat caa tta gga cgt tgg aaa gaa Glu Lys Ala Gin Glu Lys Val Phe His Gin Leu Gly Arg Trp Lys Glu 55 60 65 tta aag aaa cat aag ccg gga etc gtt ate ggt gtt ggg ggc tgt gtt Leu Lys Lys His Lys Pro Gly Leu Val lie Gly Val Gly Gly Cys Val 70 75 80 gcc tea caa gaa gga gaa cac att cgt act cgt get cct tat gtc gat Ala Ser Gin Glu Gly Glu His lie Arg Thr Arg Ala Pro Tyr Val Asp 85 90 95 100 att att ttt gga cca caa acc tta cat cgt tta cct gaa atg ate aat lie lie Phe Gly Pro Gin Thr Leu His Arg Leu Pro Glu Met lie Asn 105 110 115 cag ate aga ggt ggt aaa age tea gta gtc gat gtc agt ttt cca gaa Gin lie Arg Gly Gly Lys Ser Ser Val Val Asp Val Ser Phe Pro Glu 120 125 130 att gaa aaa ttc gac cgt tta cca gaa ccg cgt gca gaa ggt cca act lie Glu Lys Phe Asp Arg Leu Pro Glu Pro Arg Ala Glu Gly Pro Thr 135 140 145 get ttc gta tcc att atg gaa ggc tgt aat aaa tat tgc tea ttc tgt Ala Phe Val Ser lie Met Glu Gly Cys Asn Lys Tyr Cys Ser Phe Cys 150 155 160 gtc gtg cct tat acg cgt ggt gaa gaa gtc agt cgt cca gtg gat gat Val Val Pro Tyr Thr Arg Gly Glu Glu Val Ser Arg Pro Val Asp Asp 165 170 175 180 gta tta ttt gaa att gca cag ttg gca gag caa ggc gtg cgt gaa gtg Val Leu Phe Glu He Ala Gin Leu Ala Glu Gin Gly Val Arg Glu Val 185 190 195 aat tta tta gga caa aac gtg aac get tat cgt ggt gca act cat gat Asn Leu Leu Gly Gin Asn Val Asn Ala Tyr Arg Gly Ala Thr His Asp 200 205 210 gac ggt att tgt act ttt geg gaa ttg tta cgt tta gta gcc get att Asp Gly lie Cys Thr Phe Ala Glu Leu Leu Arg Leu Val Ala Ala lie 215 220 225 gat ggt att gac cgt tta cgt ttt acc acc agt cac cca att gag ttc 136852·序列表.doc -160- 420 474 522 570 618 666 714 762 810 858 906 954 1002 1050 1098 1146 1194

i SI 12421334884

Asp Gly lie Asp Arg Leu Arg Phe Thr Thr Ser His Pro lie Glu Phe 230 235 2Λ0 act gat gac att att gat gtg tac cgt gat acg cca gag ttg gtg agt Thr Asp Asp lie lie Asp Val Tyr Arg Asp Thr Pro Glu Leu Val Ser 245 250 255 260 ttc tta cqc tta cct gta caa agt ggt tct. gat cgt gtg tta tct atg Phe Leu His Leu Pro Val Gin Ser Gly Ser Asp Arg Val Leu Ser Met 265 270 275 1290 atg aaa cgc aat cat acg gca tta gaa tat aaa teg att att egg aag Met Lye Arg Asn His Thr Ala Leu Glu Tyr Lys Ser lie lie Arg Lys 280 285 290 1338 tta aga gcg gtg cgt cca gag att caa att age tea gat ttt att gtc Leu Arg Ala Val Arg Pro Glu lie Gin lie Ser Ser Asp Phe lie Val 295 300 305 1386 ggt ttc ccg ggc gaa aca gca gaa gat ttc gag caa acc atg aat tta Gly Phe Pro Gly Glu Thr Ala Glu Asp Phe Glu Gin Thr Net Asn Leu 310 315 320 1434 att gca caa gta aat ttt gat atg agt ttc age ttc att tat tea gca lie Ala Gin Val Asn Phe Asp Met Ser Phe Ser Phe He Tyr Ser Ala 325 330 335 340 14Θ2 cgt cca ggc acg cca gca gca gat atg cct gat gat gtg aca gaa gaa Arg Pro Gly Thr Pro Ala Ala Asp Met Pro Asp Asp Val Thr Glu Glu 345 350 355 1530 gag aag aaa caa cgt tta tat gtg ttg caa caa cgc att aac aat caa Glu Lys Lys Gin Arg Leu Tyr Val Leu Gin Gin Arg lie Asn Asn Gin 360 365 370 1578 gec gcg caa ttt agt ega gca atg tta ggc aca gaa cag cgc gtg tta Ala Ala Gin Phe Ser Arg Ala Met Leu Gly Thr Glu Gin Arg Val Leu 375 380 385 1626 gtg gaa gga ccc teg aaa aaa gat tta atg gaa etc aca ggg cgt aca Val Glu Gly Pro Ser Lys Lys Asp Leu Met Glu Leu Thr Gly Arg Thr 390 395 400 1674 gaa act aat cgt ate gtc aat ttt gtg ggc acg cct gat atg att ggg Glu Thr Asn Arg lie Val Asn Phe Val Gly Thr Pro Asp Met lie Gly 405 410 415 420 1722 aag ttt gtt gat ate aag ate acg gat gtg tct act aac tea ctg cgt Lys Phe Val Asp lie Lys lie Thr Asp Val Phe Thr Asn Ser Leu Arg 425 430 435 1770 ggt gaa gtc gtt aga act gaa gaa caa atg gga ett cgc gtt gtt caa Gly Glu Val Val Arg Thr Glu Glu Gin Met Gly Leu Arg Val Val Gin 440 445 450 1818 teg cca caa atg gtg att aat cgt act cgt aaa gaa gac gaa etc ggc Ser Pro Gin Met Val lie Asn Arg Thr Arg Lys Glu Asp Glu Leu Gly 455 460 4 6*5 ΧΘ66

t 9 9 r c A a y o 91 7 90 4 91 t a 9 V tat cac gcg tagtcgtgct atcccttcaa atacttaacc 1914 Tyr His Ala 136852·序列表.doc 16】·

l SI 1334884

gctctcgagt ttctcaagag cggttatttt ttatgaaaaa tttttgataa attgaccgct 1974 ctttttattg cttcatttta tgatagacag cgtgttttct gttattcatc gtatttcttt 2034 ttatttcact tcattaataa attattaaat ttcaattgct tatcaaaatt gttgtttttt 2094 gctttttttc tatttatagc atggttattt tttatacaca catggcgtat ttctccatat 2154 ttttacaaaa aactgtgact tcactctaac tattgttctc tcgtgcttta ctccatttta 2214 taaggcggtt agtttagatc atgttgtttt tacaacatat tttgaggttg tttgaaggtt 2274 gagcttattt tatagttgag gtgatgatga aaaaatttaa tcctagtata ttggcgttgt 2334 ccatatcaag tctgctactc acatccacat tgacctttgg tcaaatccag caacaagata 2394 aagcactctt tggtgtgaaa gaacatcaag aaagcctact ctttcatcag agcttagtag 2454 aacaaggtag tgataatgtg ccaatttggc gcattccgtc tttattaaga acaaaagacg 25X4 gtgtattaat tgccgccgca gataagcgtt ggcaacaccg tggtgactgg ggcgatattg 2574 ataccgcaat ccgaattagt catgatgatg ggaaaacatg gggaaatatt acaacgattt 2634 tggatttgcc atcaaagaat ggggaaaaat ccccatcagc accagatcct gtaacattca 2694 acgcgtgggg agatagacaa aattgtacaa cttactgtaa ttctgctttt ttgatcgatg 2754 ctcagatggt acaagataaa cgtaatggta gaattttttt agcagttgat atgtttgccg 2814 atggagcagg cttttttggt gtaaaagaca gtggtaatgg gcgcattaat attgatggta 2874 aacagtatcc tattttaaat gaaaagcaat cgggtcgaca ttgaaccctg tgttcttaac 2934 tctttcactt gctcaacgtt caccggaatc acgcggtcaa taaaagtcgg tttccagttt 2994 aaacccggtt gaacaatagc gtgtaattca ccaaaacgca tcaccacgcc acgttctgct 3054 tctttgacag tataaaaacc gctaacgccc cagacaattg caccaatcac cgctgcaatt 3114 ggcaataatt taccaagatt taacgatcca ccagaatgcg agttattgcg tttaccgcca 3174 ttccctgaac cgcctaattt tttcagcaga ttattaaaaa tctcttcaat atcaggtggt 3234 gattgctctt gattgttttg cttacgacct gactggttat cattccaatt cggctgaccg 3294 ccttcattat caggacgctg actctcaggt tttttctgtc cgggtttgcc ccaaggatct 3354 tgatctgaac cgttcaatga cattacgttc tccatttgtc aaaaaattgt tacttgttta 3414 aacaaattcc cacagtctaa acaaaatgtt ggattaagtc taacgaaact cgttactaaa 3474 tggggatatt ttttatgatt taaagtgccg ccaataaaaa cgcacaatgg tctcattttt 3534 acggcttact acctgacttt tgtcctatcg cttcgtcggt catgtttttt cattcacctt 3594 ataataaatg gcgtcgtgta aggtggtttc cgctaatatt cccctctgcc aagtaaaata 3654 gtgcacatct aagcactgtg tttggttaat aaaaaaagcc aagtaaaacg gcaagcggtg 3714 actaaagagg agttaactaa gtttctgcat gccagcgtac tattgactct tcttgcactg 3774 tgttcgggtt ttattgcggt gaatgaaacc ctagcgtgta tttctttgtt attgttggct 3Θ34 -162- 136852·序列表.doc i s] 1334884

tatttaagcg ttagccgagt agctattact attatctctt gtaggcatcg ttctggcact gcaacattac aagctgaatc ctaggattca ctttgttggc aaatatgaag atattttaac ccgcgttcct tgatgcaagg agtgaggaat ggggaaaaaa ttaaaacgag acagcctagg acatttgacg ggtgtacacc cgtttaccaa gtcaagacta gaatatgccg aatatcgctt aatttaaaag cattataaaa tacttcaccg caatcattga aaaccgactt cagctaaacg tcaagtgcgg tgcgtttttg tgatgatgta aatcaatcaa gatagtacca agatcccatt cgatcttcag gtggcagaaa atttcgatat ggcgtatatc ctaacatgct ggcgacaacg acgccgggtt gattaatatg agatcataga cgttgctatt atgatgttgg catagccggg ctttcatcaa aagtaaaatc tgtcataatg tattaaagac tcacaaataa attatgattt cgcacgccta ggataagcaa cagaaaagcg ataaaccaat acgtatttaa acaagaaaga gtccaaatct ctttcagttc atcqccaacc ctatccataa attatttgcg ctcgcgttgt tgggcgtgat gagtatccct ttattacata aatctttctt agttacgttt gttttatcac gttataacaa gcacaatacg tttcaaccac aaacaatgtt gacatgtttg attgcctttg ccacgaatta gaatggtgag tcaattattt taaaaacggc ggattatatg acgttgaact atgaaatctt tttagaagag gaaaaaacac agtattttgt gattgctacc ttgtgtcgtc ttgaaaccaa aaatatttac ttaccggtga atgtcgcgat cttttttgcc gagggaaaag gtgaatatta taaaggggga aaagcgttgt tgttccgctt caaaaggcgg tagtgaaccg cctttatgtt gccaaaatta aagcattgaa accataattc catttt.atgg gcttgaatgc tgtctgtacg gctgacttca agttcactgt atccattcgc caaggcattc atgttttgat cttcaaaacg cggttgtaag ccttggtaaa tcttacttaa ttgcaaagtg aaatttaaaa ttaccatgaa atcacaaata atgtccacag gtattgtact atcgaccatg ggttgtgagt tatcaacacc acgacgtgca gataatgttg ccgcaccgcg cgccgtaaca aagcgtgatg ccaacatccc aatagagcgt tggatcatgt cgggaaagac ccccaattta gcaatgggga gtcgaaaata cgagaaaaag tgcggtcatt ttagaagaag cgggaataaa tgcgccatgg cactttcttc ataaatgggt aatccaatca atgcggtata taactcggga ataatgttta agaaataatt acggttaatg tgatgttccg gtaaaatgta ataaatcacg taaaatagca tggcgatcgc aacactatta aatqctatqt t.ttaat:tatt catgaatttg 3694 actcatggga 3954 ggcaccgcag 4014 acgtaaaaaa 4074 tactattcat 4134 acccgttgat 4194 ggcagacatt 4254 ttatgcgttg 4314 agcacctaca 4374 gtggtggccg 4434 tgcacaagct 4494 actagataaa 4554 gtatgccact 4614 cacttgttga 4674 catcacattt 4734 gcgtttaaat 4794 aaatttctcg 4854 taaggctcga 4914 ggcattttcg 4974 ttgataggca 5034 aatgatcttg 5094 cgagcacccc 5154 aatagcggta 5214 ttcagctacg 5274 gagtatcttt 5334 tttcgaccta 5394 ttgtttaata 5454 ttttgcttgg 5514 cgggtgacgt 5574 aagttttact 5634 aaagactaaa 5694 136852-序列表.doc •163·

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atcgcggcga gataaaatac aacatgggca atggataaca gcgtagaatt gcgtttacca 5814 tattgtattg caccttttgc aatcaatgct ttttcatggc ggattgaaat agacagacta 5874 taaaaacgga tcgctaaaat acaggcaaaa gtgatattga taaataacat gtggttatcc 5934 ttaacataat tttattccaa taaagtggcg taatataaca aaaaatccga tgatatggca 5994 tggaaatacg cctttatttt gaacgttcat cgcttttctt tttctttatt ggttgatttg 6054 taagcataaa taaccttttt atctctcttg gttttccgct ataattgagt gaatttttga 6114 gcataattaa gagtacaaag gattttgaaa tgatgcgtac acattattgc ggtgcattaa 6174 accgtgacaa tatcggacaa gaagtaacat tgagtggttg ggtgcatcgt cgccgtgatt 6234 taggtgggtt aatttttatt gatatgcgtg atcgtgaagg gattgtacaa gtgtgtttcg 6294 atccaatata tcaagaagca ctcaccacag cagcaagttt acgtaatgag ttttgtattc 6354 aaattaaagg cgaagtgatt gcccgcccag ataatcaaat caacaaaaat atggcaacag 6414 gcgaagtgga agtgttagca aaatccctgt ctatttataa cagcgcagag ccattacctc 6474 tcgactttaa ccaaaataat acggaagaac agcgtttaaa ataccgttat ttagacttac 6534 gtcgcccaga aatggcgcaa cgtttaaaaa cacgagccaa aattaccagc tttgtgcgtc 6594 gctttatgga cgaacatggt ttcttagata ttgaaacacc gatgttga 6642 <210> 81 <211> 474 <212>蛋白筲 <213>敗血性巴斯德.桿菌 · <400> 81

Met Thr Gin Lys Leu His lie Lys Thr Trp Gly Cys Gin Met Asn Glu 1 5 10 15

Tyr Asp Ser Ser Lys Met Ala Asp Leu Leu Asn Ser Thr His Gly Leu 20 25 30

Glu Leu Thr Glu lie Pro Glu Glu Ala Asp Val Leu Leu Leu Asn Thr 35 40 45

Cys Ser lie Arg Glu Lys Ala Gin Glu Lys Val Phe His Gin Leu Gly 50 55 60

Arg Trp Lys Glu Leu Lys Lys His Lys Pro Gly Leu Val lie Gly Val 65 70 7S 80

Gly Gly Cys Val Ala Ser Gin Glu Gly Glu His lie Arg Thr Arg Ala 85 90 95

Pro Tyr Val Asp He lie Phe Gly Pro Gin Thr Leu His Arg Leu Pro 100 105 no

Glu Met lie Asn Gin lie Arg Gly Gly Lys Ser Ser Val Val Asp Val 115 120 125

Ser Phe Pro Glu lie Glu Lys Phe Asp Arg Leu Pro Glu Pro Arg Ala 130 135 140 -164- 136852·序列表.doc 1 1334884

Glu Gly Pro Thr Ala Phe Val Ser lie Met Glu Gly Cys Asn Lys Tyr 145 150 155 λ60

Cys Ser Phe Cys Val Val Pro Tyr Thr Arg Gly Glu Glu Val Ser Ara 165 170 175

Pro Val Asp Asp Val Leu Phe Glu lie Ala Gin Leu Ala Glu Gin Gly 180 185 190

Val Arg Glu Val Asn Leu Leu Gly Gin Asn Val Asn Ala Tyr Arg Gly 195 200 205

Ala Thr His Asp Asp Gly lie Cys Thr Phe Ala Glu Leu Leu Arg Leu 210 215 220

Val Ala Ala lie Asp Gly He Asp Arg Leu Arg Phe Thr Thr Ser His 225 230 235 240

Pro He Glu Phe Thr Asp Asp He He Asp Val Tyr Arg Asp Thr Pro 245 250 255

Glu Leu Val Ser Phe Leu His Leu Pro Val Gin Ser Gly Ser Asp Arg 260 265 270

Val Leu Ser Met Met Lys Arg Asn His Thr Ala Leu Glu Tyr Lys Ser 275 280 285 lie He Arg Lys Leu Arg Ala Val Arg Pro Glu lie Gin lie Ser Ser 290 295 300

Asp Phe lie Val Gly Phe Pro Gly Glu Thr Ala Glu Asp Phe Glu Gin 305 3X0 315 320

Thr Met Asn Leu He Ala Gin Val Asn Phe Asp Met Ser Phe Ser Phe 325 330 335

He Tyr Ser Ala Arg Pro Gly Thr Pro Ala Ala Asp Met Pro Asp Asp 340 345 350

Val Thr Glu Glu Glu Lys Lys Gin Arg Leu Tyr Val Leu Gin Gin Arg 355 360 365

He Asn Asn Gin Ala Ala Gin Phe Ser Arg Ala Met Leu Gly Thr Glu 370 375 360

Gin Arg Val Leu Val Glu Gly Pro Ser Lys Lys Asp Leu Met Glu Leu 385 390 395 400

Thr Gly Arg Thr Glu Thr Asn Arg He Val Asn Phe Val Gly Thr Pro 405 410 415

Asp Met lie Gly Lys Phe Val Asp lie Lys He Thr Asp Val Phe Thr 420 425 430

Asn Ser Leu Arg Gly Glu Val Val Arg Thr Glu Glu Gin Met Gly Leu 435 440 445

Arg Val Val Gin Ser Pro Gin Met Val He Asn Arg Thr Arg Lys Glu 450 455 460

Asp Glu Leu Gly Val Gly Arg Tyr His Ala 465 470 -165- 136852-序列表.doc

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<^10> Η2 <211> 4835 <212> DNA <213>敗血性巴斯谂桿8 <220>

<221> CDS <222> (407) .. (1156) <220> <223> yojB <400> 82 gtcaacgacg gggcgggtct tagaacattg gcatacgggt acgatgacac gccgtgtccc 60 agagctccat cgctccttcc caaataactt ggtttggatg cacccattag atgcgaaaaa 120 acgtggttta cgtcatggcg ataaagtgaa gatcagctca cgtcgtggcg aaatgatttc 180 tcacttagat acccgtggac gtaataaagt cccacaaggc ttagtttaca ccactttctt 240

tgatgcaggt cagttagcaa actatctcac tttagatgcg acagacccaa tttcaaaaga 300 aacggacttc aaaaaatgtg cggtcaaagt ggaaaaagcg taacacgtta aatttaatga 360 ggaacgaccg cactttgctt tcagtaaagt gcggttggaa agtcga atg aaa aaa 415

Met Lys Lys 1 aca gtt gtg aat cct gaa cgt cgt cga ttt ttt aaa gag get aeg ege 463

Thr Val Val Asn Pro Glu Arg Arg Arg Phe Phe Lys Glu Ala Thr Arg 5 10 15 act gca ggc ggg ttg gca ggg gtg act ttg etc ett ggt ttg caa caa 511

Thr Ala Gly Gly Leu Ala Gly Val Thr Leu Leu Leu Gly Leu Gin Gin 20 25 30 35 aag cag agt ett geg ege gaa ggc gtg geg tta ege cca cct ttt gee 559

Lys Gin Ser Leu Ala Arg Glu Gly Val Ala Leu Arg Pro Pro Phe Ala 40 45 50 ett gag aat gag aaa geg ttt tet get geg tgc att cgt tgt ggt cag 607

Leu Glu Asn Glu Lys Ala Phe Ser Ala Ala Cys lie Arg Cys Gly Gin 55 60 65

tgt gta caa gee tgt cca cat gag atg ttg cat ett gee tea ctg att 655

Cys Val Gin Ala Cys Pro His Glu Met Leu His Leu Ala Ser Leu He 70 75 80 tea ccg atg gaa gca ggt aca ccg tat ttc att geg ege gat aag ccc 703

Ser Pro Met Glu Ala Gly Thr Pro Tyr Phe lie Ala Arg Asp Lys Pro 85 90 95 tgt gaa atg tgt gtg gat att cct tgt gca aaa gee tgc cca acc ggt 751

Cys Glu Met Cys Val Asp lie Pro Cys Ala Lys Ala Cys Pro Thr Gly 100 105 110 115 gca ttg gat aat caa gca aca gaa ate gat gat geg cgt atg ggg tta 799

Ala Leu Asp Asn Gin Ala Thr Glu lie Asp Asp Ala Arg Met Gly Leu 120 125 130 get gtc ctg cca gat cat gaa act tgt ctg aac tgg caa ggt tta ege 847

Ala Val Leu Leu Asp His Glu Thr Cys Leu Asn Trp Gin Gly Leu Arg -166 - 136852-序列表.doc 1334884 135 140 145 tgt gat gtg tgt tat cgc gtc tgt ccg ctg att aat aaa gcg att aeg 895

Cvs Asp Val Cvs Tyr Arg Val Cys Pro Leu ne Asn Lys Ala He Thr ' 150 * 155 160 tta gtg atg cat cgt aat gag cgt aeg ggt aag cac gee gtc ttt ate 943

Leu Val Met His A **g Pft* Glu Thr Gly Lys His A].今 Phe 1.1® 165 170 175 cca aca gtg cat tee gaa gee cgt aca gga tgt ggc aaa tgt gaa gaa 991

Pro Thr Val His Ser Glu Ala Cys Thr Gly Cys Gly Lys Cys Glu Glu 180 185 190 195 get tgc gtt eta gaa gaa gcg gca ate aaa gtg tta ccg atg gca tta 1039

Ala Cys Val Leu Glu Glu Ala Ala lie Lys Val Leu Pro Met Ala Leu 200 205 210 gcg aaa ggc atg tta ggt aaa cat tac cgt tta ggt tgg gaa gag aaa 1087

Ala Lys Gly Met Leu Gly Lys His Tyr Arg Leu Gly Trp Glu Glu Lys 215 220 225

gaa aaa gee ggg cat tee ett gcg cca gaa ggc att att tet etc ccg 1135

Glu Lys Ala Gly His Ser Leu Ala Pro Glu Gly lie lie Ser Leu Pro 230 235 240 act egg tta ccg gag age ttg taatggcaaa ttcaccaaaa tatgcgggta 1186

Thr Arg Leu Pro Glu Ser Leu 245 250

aagaagcacg taacccagct tgaaaggcaa ttatggcaga tgattgtggt cgttaaacat cggcaaaact egetaagegg taatttacgg ttattgtcga ttggtgcgaa tggattgeta agagtgaaag tttgccctga gggagtgatc caatgccaac aqccttccac agaaaagtta gagtat仁ett ttacagtagt aagtctcgcg ggtgctttat cgtgacagat cccacgaagt gttattaett tttcggtgcg gcatggttgg aggacttttt taacgtctgt cccacttgtg aaaagtattt aaaatgaaaa gaagtcaaag aatacaccaa ggctggtggt gccatgtttt agtcttttgc accggtttta gccattttag gcgtccgctt ttacgctatg tgggaatggc acagtttggc tgtgggcatc cgtatcaaag ccagaaccac ctttcaaaag atttttacaa aaacaatgct tgggcaaaag aagaaagtgg acgccaatcg taagcggacc ttgatttgat tgcctaccat ggagtcgcgt ggctcagacg egatettagt tcaatccggt ttgttcttgc tatgcccaat ttgageateg aagtgttacg attgtatcag cacgatttaa aattttgacc tttacaagat cttqgcgccq ctttttgttc ttattttggg cccgatgact gaccgcgttg tttctgtgct taaattagaa gatgattttg tgcagcacta ggtgttttta aggtgcagcc ccaacaatgt cgatccatta ctgtggacgt tcattcagct gcactttttg tcacctgaaa tggcgacgtt 1246 gtgtggatct 1306 gatcctttga 1366 ttgggtgccc 1426 tgggtttgtc 1486 attcgtcaat 1546 ttaggcagtg 1606 ggtcgtgcgt 1666 tttgatttat 1726 tatggtgtga 1786 gataattgea 1846 catgcaaaaa 1906 tgtatcgacg 1966 aatcattcgg 2026 ccttcacggt 2086 atatcgcgcc 2146 •167- 136852-序列表.doc l 51 1334884

accgatggtg ccacacgcga cgaaaaacta tcaggtgacg aaaaatatca accaatgttt 2266 aacttgtcat agcccagaag cctcacgtgt tactggtgca acacgcatca gtccaaccca 2326 ctttatggat cgtgatggta acattgtcgg aggcacctca cctcgccgtt acttctgttt 2386 acaatgccat gtctctcaat ctgatgttga gccgattatt caaaatgaat ttaaaccgat 2446 ggctggtttt ggtaaataag ttaaggacgg aactatgttg aacttaatta aacgcttttg 2506 gaaatggttt cgctcgccca gtcgtattgc cgttggtacc ttgattaccc ttgcttttat 2566 tgcaggtatt gtctcttggg ttgggtttaa ttatggctta gagcaaacca atacagagga 2626 attctgtgtt agttgtcaca gtaatgatgt gtatccagaa tatttacata cggcgcatta 2686 tttaaatcgc agtggagtaa aagccacttg tcctgattgc catgtaccgc acgaatttat *2^46 tccaaaaatg atccgtaaag tccaagccag tcgagaagtg tatgcgcatc ttatgggtta 2806 tacggatacc attgataaat ttaactcccg tcgtttgcat atggcagaac gtgaatgggc 2866 acgattaaaa gccaataact cgcaggaatg ccgtaactgc cataactttg agaatatgga 2926 ttttagtcag caaaaaacgg tggcggaaaa aatgcatgca cttgctataa aagaagaaaa 2986 aacctgtatt gactgtcaca aagggattgc gcaccaatta ccggatatga gcggtgttga 3046 gtcgggtttt agtacggaac aaaaataaca cttttcttgt cctcccacag gaaatctgca 3106 tcgtattcag gtgcagattt ttttattttt acgatcatcc tcacaacctt ttcgctgata 3166 aataggatac aacgctagct acacgctatc ttgctgcttt ttgtgttgtt gtgagggatt 3226 atgtcatctt atctgcctct tttattacgt ttgttacaaa ttccgcgttt gggttcgctg 3286 gcgatccagc gtttattgga gcacatcagt ccagcggaat taatggaata tgatacaaaa 3346 gcctttcaac agataggctg gacggcacag cagattcagc gttggtttac gcctgagaac 3406 cgttatattg atcccgcgtt agcctgggta aacgaacaac aacatattgt ggattggttt 3466 gatcctcatt atcctccctt gttaaagcaa acagaagagg caccacttgt cttgtttgtg 3526 aaaggggaag tggctacgct atctgcacag caagtggcga tcgtgggcag tcgtcattgt 3586 tcacgttatg gggaatactg ggcaaattat ttcgccactc aactggctta tgccgatatt 3646 gtggtgacca gtggtttagc gttaggtatt gatggtttct cacatcaagc ggtagtggat 3706 atccacggga agacgattgc agtattaggc agtggtttag aggtcattta tccgaaaaaa 3766 caccgaggtt tagccgaaaa aattattgaa catcaaggtg cgttagtgtc tgaattttta 3826 cctttccaac cccctgtggc agaaaatttt ccacgtcgta atcgcattat tagtggttta 3886 tctttgggga cattagt.cat tgaagcctca gaaaacagtg gttctttaat tactgcccgc 3946 catgetttag agcaaaatcg agatgttttt gcgttaccgg ggcagattca gcatggattt 4006 agccaaggct gtcataaact gattaaacag ggggcaatat tagtcgaaag tattcaggat 4066 attttagaac acctcccgcc ttattcqcat tqtqccatac caacqttacc tcqtacaQag 4126 -168- 136852-序歹 lj 表.doc 1334884

aacgcctttt ctcaacaagt aacagacaca tcaaccatca atactgcgcg gataacgcca 4186 gaacatccgg aattatatgc caaaattggc tatatgcccg tcagtattga tgtattagcc 4246 caacaagtca atctacctat tgatacctta ttagtacagc ttttaacctt ggaattgcaa 4306 gacttgattg ttgctgaaaa tggattatat cagcgcaaat aacggaaaga gtaaccacaa 4366 atgagaaagg actgtgttga aacggctatc aacatcagtc cttgcagtat attatctcat 4426 atgajacgag acggcagttt catgagatta atagagacta aacgcttgta aatacggtag 4486 tttgcctcgt tgtagcattt tctcaatatt gcctttgtga tcatcacgac ctaagcctcg 4546 tagctcaata ttgataccaa tacttttgtc ataaaaaatt tgattcgccg tttggttttc 4606 tttgctggtc acactacgtc gggcaccaac attaatcgcc caacagcaag tgctgtattc 4666 tagtcccaaa tattgctcaa caggctttct caacgcaaga tcttgataat gacgtgctac 4726 caccgcccat tgatccgtga gtgcccatgc cacagttaga ccaagctgtt ttatgtcttg 4786 tccgtagcgg ttcgccgctg aggtgaggtt ttggtcaata tattgttga 4835 <210> 83 <211> 250 <212>蛋白質 <213>敗血性巴斯德桿菌 <400> 83

Met Lys Lys Thr Val Val Asn Pro Glu Arg Arg Arg Phe Phe Lys Glu 1 5 10 15

Ala Thr Arg Thr Ala Gly Gly Leu Ala Gly Val Thr Leu Leu Leu Gly 20 25 30

Leu Gin Gin Lys Gin Ser Leu Ala Arg Glu Gly Val Ala Leu Arg Pro 35 40 * 45

Pro Phe Ala Leu Glu Asn Glu Lys Ala Phe Ser Ala Ala Cys lie Arg 50 55 60

Cys Gly Gin Cys Val Gin Ala Cys Pro His Glu Met Leu His Leu Ala 65 70 75 80

Ser Leu lie Ser Pro Met Giu Ala Gly Thr Pro Tyr Phe lie Ala Arg 85 90 95

Asp Lys Pro Cys Glu Met Cys Val Asp lie Pro Cys Ala Lys Ala Cys 100 105 110

Pro Thr Gly Ala Leu Asp Asn Gin Ala Thr Glu lie Asp Asp Ala Arg 115 120 125

Met Gly Leu Ala Val Leu Leu Asp His Glu Thr Cys Leu Asn Trp Gin 130 135 140

Gly Leu Arg Cys Asp Val Cys Tyr Arg Val Cys Pro Leu lie Asn Lys 145 150 155 160

Ala He Thr Leu Val Met His Arg Asn Glu Arg Thr Gly Lys His Ala 165 170 175 -169- 136852-序歹丨j表,doc

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Val Phe lie Pro Thr Val His Ser 180

Cys Glu Glu Ala Cys Val Leu Glu 195 200

Met Ala Leu Ala Lys Gly Met Leu 210 215

Glu Glu Lys Glu Lys Ala Gly His 225 230

Ser Leu Pro Thr Arg Leu Pro Glu 245

Glu Ala Cys Thr Gly Cys Gly Lys 185 190

Glu Ala Ala He Lys Val Leu Pro 205

Gly Lys His Tyr Arg Leu Gly Trp 220

Ser Leu Ala Pro Glu Gly lie He 235 240

Ser Leu 250

<210> 84 <2ll> 3494 <212> DNA <213>敗血性巴斯德桿逋

<220>

<221> CDS <222> (2411)..(2719) <220> <223> yyaM <400> 84

caagactgcg 60 ggaagcccaa 120 agtcgatgat 180 tgatttagcg 240 agcacaagca 300 ggatgtactg 360 acaagaagca 420 agcgttaatt 480 gagacatgtt 540 gacaatgaca 600 gccttttgta 660 ttgttgtgcg 720 tactatttac 780 agaaaatatt 840 tcaagaatgg 900 cactatgtgc 960 gtcaacgatc cgaatacgac ggaagaaaac tatatgcaag tcatttatgg cgtctattcg aagctgcgac gcattgcgcc gcgatacttg ctcatgcgac gaaaatgctt tacgcgaata tggttgttat ctaggcacag cttttcaatt attttagatt atagtgcaga tgcaaaagca ctcggcaaaa atattggtga gaaggtaaac caacattacc gttattacac gccatgcatc acggcaatcc aaattgattc gcgaagcgat tgagcaaggg ggtaagcgtg atattttaga acaattatga cagaacataa atccctcgac tatgcgatga tgcgcgctaa caaaaagccg ttgatgcgat tgcattattg cctgaaaatg aatataaaca tcattagctt acctatccgt cgatcgcgct tattaaccac ttaataaggc agcgtaacga ccgcctaaag tgcggtcatt tatttagtaa ttttaaacac gaacaaaaca tccctacgaa aaaaacacgc aaaggcaaag atcctcacgc cgcgaaaaat tatccctacc aaatgggcat aacaaatCgt tattgcattc ccttgctcgg gagaagtaac ggaggcaatt catgcttcag gtattgaatt ttctacaacc caaacattca cccattaaaa gaatatctca Ctcgtaaaga cgttttgcgg aaaaatgggg cattccgttt attgatgccg attatgatcg tttgaccgtg ccaaaggcat ggaagatgag ccagagcgtg gtattcqttg tttgatatgc gttztgaaaa agccgcagaa catgcacaca acaatggttt -170- 136852-序列表.doc

! SI 1334884 actagctgct taggtatttc acgttggaaa gacatgaacc aaatcaacgg ttgtggacac 1080 cgtgccgccg aaaaatatga tgatgtagtg tattgggatt ataactggcg taaaggtggt 1140 ggctcacaac gtatgattga aatcagtaag cgtgaacgtt tttaccagca agaatattgc 1200 ggttgtgtgt attctttgcg tgacacgaat aaatggcgtg aagcaaacgg acgccaaaaa 1260 attgaaattg gcaaattgta ttattccgcc gattaaaaca accaagtaaa ccatcgtgcc 1320 gtcatataac atggcggcac ttttttaggc tttatatatg caacgttttc gccaatcttt 1380 cgaaacctaa tgctaacgcc tcttcactaa tatttaatgc tggggcaaaa cgcaatacat 1440 taggacccgc cactaaaatc attaagccat tatctgctgc ttttttgaca aattcgctcg 1500 ctcttccgtg atattgctcg ataagttctg caccaattaa caaaccttcg ccacggattt 1560 ctttaaataa gccacattgc tgattaattt cttgcagctt ttgcataaat ttttcagaag 1620 tgcgctgaat tttttgcaag aatggtggtg ctgaaatgat atcaatcact ttttctgcca 1680

cggcacaagc gagaggattg ccaccaaagg tggtgccatg cacgccaggt gcaaaacttt 1740 tggcgatctc atgcgttgtc aacgaatgag aaaattgtga cagattgggc tccaacggga 1800 cgtgcggatg agattgggga tacgcaagta cgttatgaaa ttgagcaggt gagttctagc 1860 aattatagtg cactgtttgt ctccatttta cagatgaaac gtaatgaggt ggtatttagt 1920 ccacatttag cggataaaca acgttatagt tctgatcgct tgaaccaact tgttggtgaa 1980 ttagatgcgt cttatcgtaa acaagtccgt gatttgaata acagcggatt gatgccaatt 2040 cagtctgtat tcggtacgga cagtaatggg cgtactgctc tcgtgttagg cgcacccttt 2100 aatcatgcgt ggacaaaact aggacaagtt ttaccgcagc ttgaatttga tattaaagat 2160 gaaattattg gtcgtggggt aagggagtta aaataccgtc cagctggagc aaaaagttgg 2220 tggtggccat ttggtcgtgc tgaaggcagt agcggactga aaacaggtac ctattttatg 2280 cagttaagcg ccttagggaa gcaaagtgcg gtggtgatga ccgatgatga tggcaatgcg 2340 ttatctgggg agcaagctca ggcgctttat caagcattac aaaatctctt agcgaaataa 2400

tacagtcaag atg act aaa etc agt ate cag ega gat aac ttg att tgt 2449

Met Thr Lys Leu Ser lie Gin Arg Asp Asn Leu lie Cys 1 5 " 10 ttg agt tat gtc gca tta atg gga ttc ggc ttt ccc att atg cgt tat 2497

Leu Ser Tyr Val Ala Leu Met Gly Phe Gly Phe Pro He Met Arg Tyr 15 20 25 atg agt att cat ttt gat aca tta aat aat Met Ser lie His Phe Asp Thr Leu Asn Asn 30 35 tea ggg ggc age gtt ttt Ser Gly Gly Ser Val Phe 50 gag eta aca tet teg ggg aac get Asn Ala 40 att tta gcc tgt ttt ttt lie Leu Ala Cys Phe Phe 55 get ggc gtc cag tcc gtt gtt ege ttt etc 2S45 Val Arg Phe Leu 45 tat tat ege get 2593 Tyr Tyr Arg Ala 60 geg atg ttg ccg 2641 -171 - 136852·序列表.doc is? 1334884

Glu Leu Thr Ser Ser Gly Ala Gly Val Gin Ser Val Ala Met Leu Pro 65 70 75 agt tea agt tta ggt ttc tta ata ttg aaa act gta cca tet ttt tea 2689

Ser Ser Ser Leu Gly Phe Leu lie Leu Lys Thr Val Pro Ser Phe Ser 80 85 90 tac gtt aca ate tea aca ett aat ege gtt tgaccttccg atttttgata 2739

Tyr Val Thr lie Ser Thr Leu Asn Arg Val 95 100

gtcaaagact actgagtaac gcttgtagtc gegtgaateg actgttacat aageegatat 2799 gtcagaataa gtactgccgg tatategtet taatetaaga ttaagcttgc cacttttgtt 2859 cgataaagcg tcaaacgaaa gcacgacttt accgtccttg acttccacct gatcttcaat 2919 gageaettga ettagtgega ccaatcgacc gttggcagtc agtgtcgcaa tgccgtgatc 2979 cgtatcaagc gttacaccgc tattttttcc ccagttttta ttgagctttt cactatgttt 3039 cagtaagttt ctgccaccaa tctgcaactg attaaaetta gcttcaagcg tttcactctt 3099 gactgcaagc gacttgtttt cattgctaac cgtctgctca agtgctgtga ttttggatgt 3159 taaatctaac ttagttgcat tgacttcatc agtccattct gactttaact cttttctcgc 3219 aagtgacgcc acttcatctt tgctagcttt cgtttctttt aagtcagaaa tgccactagt 3279 attttgcgcc actttagaat cgagcgtttc tagttttgta gagaaagatt tgtctttttc 3339 getageegtt ttttgaatta gctgtatttc actttcgctc aatccaactc tageagttag 3399 actgtctagc ttgtcagcag tagatttatt cacagtcgct tgtgattgct tgtgttgaat 3459 aatatccgcg cttacttccg agatagccac gtega 3494 <210> 85 <211> 103 <212>蛋白質 <213>改血，丨生巴期漶桿菌 <400> 85

Met Thr Lys Leu Ser lie Gin Arg Asp Asn l»eu He Cys Leu Ser Tyr 15 10 15

Val Ala Leu Met Gly Phe Gly Phe Pro lie Met Arg Tyr Met Ser lie 20 25 30

His Phe Asp Thr Leu Asn Asn Asn Ala Val Arg Phe Leu Ser Gly Gly 35 40 45

Ser Val Phe lie Leu Ala Cys Phe Phe Tyr Tyr Arg Ala Glu Leu Thr 50 55 60

Ser Ser Gly Ala Gly Val Gin Ser Val Ala Met Leu Pro Ser Ser Ser 65 70 75 80

Leu Gly Phe Leu lie Leu Lys Thr Val Pro Ser Phe Ser Tyr Val Thr 85 90 95 lie Ser Thr Leu Asn Arg Val • 172· 136852-序列表.doc

1 SI 1334884 <2I()> 86 <211> 19 <212> DNA <2丨3>人迻的序列 <22()> <223>人造序列的說明：引子 <400> 86 aggccgglac cggccgccl 19 <210> 87 <211> 19 <212> DNA <2丨人造的序列 <22()> <223>人造序列的說明：引子

<4()0> 87 cggccgglac csgcctag» 19 <2I0> 8X <2!l> IK <2Ι2> Ι)ΝΛ <2 Π>人造的序列 <22()> <22.3>人造序列的說明：引予 <400> 88 catggluccc yltctaac 18 <210> 89 <21l> IK <212> DNA <2丨3>人迻的序列

<220> <223>人造序列的說明：引子 <400> 89 clagjzlaccl acaacctc

IX <2i(i> y〇 <21I> 119 <212> DNA <2 1人造的序列 <22()> <221> <222> <223>人造序列的說明：轉因插入 <220> 136852-序列表.doc -173· 1334884 <221>mise 特微 <222>25 <223>心六或'「或(ΐ或C <22U> <221>niist·特微 <222>27 <22>Ν=Λ 或 T 或 G 或 <220> <221> iuisc—特微 <222>29 <223> Ν=Λ或丨或ti或C <22〇>

<22l>misL·特徵 <222>3I <223> N=A或T或G或C <22〇> <221> inis。—特徵 <222>33

<22.3>Ν=Λ 或丫或(i 或 C <22i)> <22l>nnsc <222> 35 <22.V、Ν=Λ或丨或U或C <220> <221> misc_#i3; <222>37

<223>Ν=Λ 或T 或(i 或 C <22()> <22l>misc <222> V) <223> Ν=Λ或T或(;或C； <22〇>

<22丨〉misc特徵 <222>4I

<2U> Ν=Λ或T或(丨或C <220> <221>

<222、·η ~ c22.’、> Ν=Λ或T或(丨或C <22()> <221> 11】丨51：_特徵 <222>45

<223>Ν=Λ 或T 或(i 或 C <22〇> <221>misc <222>47 <223> Ν=Λ或丫或(丨或C <220> <221〉iniscj 寺徵 <222>49

<223>Ν=Λ或·丨或G或C 136852-序列表.doc -174- 1 Si 1334884 <22ii> <22\> <222> 51 <22.V* Ν=Λ或 Γ或(;或C <22〇> <22 丨> 特徵 <222> S3 <22>~=八或'丨_或〔)或(； <22〇> <22丨> 丨丨1风_特微 <222> 55 <223> N=AAT或Ci或C <220> <22l>misc>Hl <222>57 <22.3>〜=八或1或(丨或(； <220>

•：22l>misc -·222> 59 —

<22.V> Ν=Λ或Τ或(;或C <22〇> <22丨〉misL_特微 <222>61

<223> Ν=Λ或T或(;或C <22(»> <221> -222> (>3 <223>N=A 或Τ 或(;或C， <220> <221 > <222>65 <223> N=Λ或T或(;或Γ <22U> <m>inise 特啟 <222> r,7

223> Ν=Λ成T或(;或C

22Π> <22 !> miscJliS: <222>69 <22> Ν=Λ或T或U或C <220>

<221> misc_vti53: <222>7I Ν=Λ或T或(i^t· <22()> <221 > <222> 73

Ν=Λ或「或或C 136852-序列表.doc -175· 151 1334884 <220> <22l>mist：J寺徵 <：222> 75

<223>Ν=Λ 或T 或(;或 C c22D> <22\> <222>77 或丨‘或(;或(； <22〇> <22丨>"iisc特微 <222>79 ?^=八或1’或U或C’

-22l>miscJiM <222> XI

Ν=Λ或T或(Ϊ 或C <220>

<22l> misc_特徵 <222>X3 <22+’，> N=A或T或(Ϊ或C -：：0> 〈22丨·⑴ise特饺 • 2U> H5 *:22> Ν=Λ或 Γ或(;或C <22()> <22丨> misc特微 <222>87 、·223> Ν:Λ或 Γ或(;或〔· • ：21> nnsL>m '：222> X1)

Ν=Λ或T或G或C <220> <22 丨 > 1111、1：_特徵 <222>91

<:22> Ν=Α或Τ或(;或C

<22\)> <22\> <222> ^ 或T 或(;或 Γ ctaggtacct acaacctcaa gcttnknknk nknknkr.knk nknknknknk nknknknknk 60 nknknknknk nknknknknk nknknknknk nknkaacctt ggttagaatg ggtaccatg 119 <2II> IS <2I2> DNA <2丨:?>人迮的序列 <22"> <22>人迻序刊的說明：引子 •176· 136852-序列表.doc 1 S1 181334884 <400> 91 Uicctacaac clcaagul <2Π)> 92 <21l> IX <2I2> Ι：)ΝΛ <2Π>人造的序叫 <22li> <223>人造序列的說明：引子 <40()> 92 lacccutlct auccciagc 18

<2I0> 93 <21l> IV <212> Ι)ΝΛ <2丨3>人造的序β <220> <223>人造序列的說明：引子 <4(J(i> 93 lacclaaiac ctcaagctt <2\()> l)4 <211> 21) <：212> DNA <2丨3>人迳的序列 <22()> <223>人造序列的說明：引子 <40()> 94 Iciccuittct uaccaagctt <2H)> 95 <21 ]> )κ) <2\2> ])ΝΛ <2 Π>人造的序列 <220> <223>人造序列的說明：引子 <400> 95 ggca»agcal lacgclgac <210> <211>27 <212> ΟΝΛ <213>人造的序列 <220> <22.3>人造序列的說明：引子 <4()1丨> 96 ggC|i*il：UlCti UiiUlUC 19 20 19 27 136852·序列表.doc -177· IS! 1334884 <2 Η» ^)7 <2ΙΙ>5.Ή <2Ι2> DNA <2丨3>胸膜肺炎放绵桴菌 <22〇> <223> at^Ki

<400> V7 tgttgagctt cgtgagatct agttaatgaa caaccgtttt taaactagat tccacaagtt agttgtagat agataatgcg aagcattaca ggtttagtag ggtcgaaagg caagtaacgg gattaggcga atgattaatg cgttccgaaa gaaaatacga tgtcacaaaa gacgatgaat tagatacgaa ttattggata gtttacttgt aacctagctt ctgaacaagc ggtacattaa tcgatgaatt aatgaattaa acgaaattgt cgtaagcttt taccaaaatc taggcttaaa 60 taatccggaa atggaacgta tcgtgggcgc 120 cggagaagtg gatgcggttt acgtcgctta 180 acctgttatc gcacagttac ttccgttacc 240 aggttcatgg gattatattt atgaaccgaa 300 tcgttattta gaaactcagg tataccaagc 360 cgctcgaatg gtagcgatga aagccgcaac 420 acaattagtg tataacaaag ctcgccaagc 480 tgcgggtgcc gcagcaattt a 531

<2lli> 9S <21l> 25 <：212> DNA <2丨3>人造的序叫 <220> <223>人造序列的說明：引子 <4()0> 98 tclcailtcc cttgctgcgg CilCCC <2\ii> Ψ) <21l> 20 <2I2> DNA 人透的序M <220> <223>人造序列的說明：引十 <400> 99 ggiiltacagc cggatccggg <2Ι0> ΠΗ) <2Π> 1〇λ4 <2Ι2> Ι)ΝΛ _-2丨> 欧血蚀巴斯德桴菌 25 2() <22()> <223> capSir； <22()> <221>C])S <222> (1)..( ΠΠ2) 136852-序列表.doc 178- 481334884

<400> 100 atg ttt aaa aat aaa aca ctt tta att aca ggt gga acg ggt tct ttt Met Phe Lys Asn Lys Thr Leu Leu He Thr Gly Gly Thr Gly Ser Phe 1 5 10 15 ggt aat get gta etc aaa cgt etc tta gaa aca gat att ega gaa att Gly Asn Ala Val Leu Lys Arg Phe Leu Glu Thr Asp lie Arg Glu lie 20 25 30 cgt gtt ttt teg cgt gat gag aag aaa caa gat gac atg egg aaa aaa Arg Val Phe Ser Arg Asp Glu Lys ^ys Gin Asp Asp Met Arg Lys Lys 35 40 45 tat aat gat gca aaa tta aaa ttt tat att ggc gat gtt cgt gac tac Tyr Asn Asp Ala Lys Leu Lys Phe Tyr lie Gly Asp Val Arg Asp Tyr 50 55 60 gat agt att tta aat gcc teg ega ggt gtt gac tat att tat cat get Asp Ser lie Leu Asn Ala Ser Arg Gly Val Asp Tyr lie Tyr His Ala 65 70 75 80 gcc gca tta aag caa gtg cct tea tgc gag ttt tat ccg tta gag gca Ala Ala Leu Lys Gin Val Pro Ser Cys Glu Phe Tyr Pro Leu Glu Ala 85 90 95 gtg aaa acc aat att tta ggt acg gca aat gtc tta gaa gcc gcc ate Val Lys Thr Asn lie Leu Gly Thr Ala Asn Val Leu Glu Ala Ala lie 100 105 110 caa aac cag ata aaa ege gtc gtc tgt ctt age aca gat aaa geg gtg Gin Asn Gin lie Lys Arg Val Val Cys Leu Ser Thr Asp Lys Ala Val 115 120 125 tac cca att aat geg atg ggc att tct aaa gca atg atg gaa aaa gtc Tyr Pro lie Asn Ala Met Gly lie Ser Lys Ala Met Met Glu Lys Val 130 135 140 ate ate gca aaa teg cgt aac eta gaa ggc aca cca acg aca ate tgt lie lie Ala Lys Ser Arg Asn Leu Glu Gly Thr Pro Thr Thr lie Cys 145 150 155 160 tgt act ege tat ggc aat gtc atg gca teg cgt ggt teg gtt ate cca Cys Thr Arg Tyr Gly Asn Val Met Ala Ser Arg Gly Ser Val lie Pro 165 170 175 tta ttt gtc gat caa ata cgt caa ggc aag cct ttt act att act gat Leu Phe Val Asp Gin lie Arg Gin Gly Lys Pro Phe Thr lie Thr Aisp 180 185 190 cct gag atg aca ege ttt atg atg aca ttg gaa gat get gtg gat tta Pro Glu Met Thr Arg Phe Met Met Thr Leu Glu Asp Ala Val Asp Leu 195 200 205 gtc eta tat gca ttt aaa aat ggt caa aat ggt gat gtt ttt gta caa Val Leu Tyr Ala Phe Lys Asn Gly Gin Asn Gly Asp Val Phe Val Gin 210 215 220 aaa gcc ccc gca gca acc att ggt acc ctt gcc aaa gca att acc gaa Lys Ala Pro Ala Ala Thr lie Gly Thr Leu Ala Lys Ala He Thr Glu 225 230 235 240 tta tta tct gtc cca aat cac cct att tcc att ata ggt acg cgt cat Leu Leu Ser Val Pro Asn His Pro lie Ser lie He Gly Thr Arg His 96 144 192 240 288 336 3Θ4 432 480 528 576 624 672 720 136852·序列表.doc -179- 768 1334884 245 250 255 qga gag aaa gca ttc gaa get tta tta age cgt gaa gaa atg gtt cat 816

Gly Glu Lys Ala Phe Glu Ala Leu Leu Ser Arg Glu Glu Met Val His 260 265 270 gca att aat gaa ggt aat tat tat ege ate cca gee gat caa ege agt 864

Ala He Asn Glu Gly Asn Tyr Tyr Arg lie Pro Ala Asp Gin Arg Ser 275 280 285 uta aat tac agt aaa tat gtc gaa aaa ggg gaa cca aaa att acc gaa 912

Leu Asn Tyr Ser Lys Tyr Val Glu Lys Gly Glu Pro Lys lie Thr Glu 290 295 300 gtc acc gac tac aac tea cat aat act gag cgt ttg act gtc aag gaa 960

Val Thr Asp Tyr Asn Ser His Asn Thr Glu Arg Leu Thr Val Lys Glu 305 310 315 320 atg aag cag tta ctg ett aaa ett gaa ttc ata cag aaa atg att gag 1008

Met Lys Gin Leu Leu Leu Lys Leu Glu Phe lie Gin Lys Met lie Glu 325 330 335

ggt gaa tac ate tea ccg gag gta ta 1034

Gly Glu Tyr lie Ser Pro Glu Val 340 <210> 101 <211> 344 <212>蛋白钎 <213>欧血性匕斯德悍谪 <400> 101

Met Phe Lys Asn Lys Thr Leu Leu lie Thr Gly Gly Thr Gly Ser Phe 15 10 15

Gly Asn Ala Val Leu Lys Arg Phe Leu Glu Thr Asp lie Arg Glu lie 20 25 30

Arg Val Phe Ser Arg Asp Glu Lys Lys Gin Asp Asp Met Arg Lys Lys 35 40 45

Tyr Asn Asp Ala Lys Leu Lys Phe Tyr lie Gly Asp Val Arg Asp Tyr 50 55 60

Asp* Ser lie Leu Asn Ala Ser Arg Gly Val Asp Tyr He Tyr His Ala 65 70 75 80

Ala Ala Leu Lys Gin Val Pro Ser Cys Glu Phe Tyr Pro Leu Glu Ala 85 90 95

Val Lys Thr Asn lie Leu Gly Thr Ala Asn Val Leu Glu Ala Ala lie 100 105 110

Gin Asn Gin lie Lys Arg Val Val Cys Leu Ser Thr Asp Lys Ala Val 115 120 125

Tyr Pro lie Asn Ala Met Gly lie Ser Lys Ala Met Met Glu Lys Val 130 135 140 lie lie Ala Lys Ser Arg Asn Leu Glu Gly Thr Pro Thr Thr He Cys 145 150 155 160 • 180- 136852·序列表,doc i si 1334884

Cys Thr Arg Tyr Gly Asn Val Met Ala Ser Arg Gly Ser Val lie Pro 165 170 175

Leu Phe Val Asp Gin lie Arg Gin Gly Lys Pro Phe Thr lie Thr Asp 180 185 190

Pro Glu Met Thr Arg Phe Met Met Thr Leu Glu Asp Ala Val Asp Leu 195 200 205

Val Leu Tyr Ala Phe Lys Asn Gly Gin Asn Gly Asp Val Phe Val Gin 210 215 2^0

Lys Ala Pro Ala Ala Thr He Gly Thr Leu Ala Lys Ala He Thr Glu 225 230 235 240

Leu Leu Ser Val Pro Asn His Pro lie Ser lie He Gly Thr Arg His 245 250 255

Gly Glu Lys Ala Phe Glu Ala Leu Leu Ser Arg Glu Glu Met Val His 260 265 270

Ala lie Asn Glu Gly Asn Tyr Tyr Arg lie Pro Ala Asp Gin Arg Ser 275 280 285

Leu Asn Tyr Ser Lys Tyr Val Glu Lys Gly Glu Pro Lys He Thr Glu 290 295 300

Val Thr Asp Tyr Asn Ser His Asn Thr Glu Arg Leu Thr Val Lys Glu 305 310 315 320

Met Lys Gin Leu Leu Leu Lys Leu Glu Phe lie Gin Lys Met lie Glu 325 330 335

Gly Glu Tyr lie Ser Pro Glu Val 340

<210> 102 <211> 4931 <212> DNA <213 >畋血丨生巴斯达保谪 <220> <223> fhaB2

<221> CDS <222> ⑴…（4929〉 <220> <221> ,nisc <222> < 2 2 3 > X“ii二任Η或衣知的胺居敢 <400> 102 atg aac aaa aat cgt tac aaa etc att ttt agt caa gtc aaa ggt tgt 48

Met Asn Lys Asn Arg Tyr Lys Leu lie Phe Ser Gin Val Lys Gly Cys 1 5 10 15 etc gtt cct gtg gca gaa tgt att aac tea get att age aat ggt tea 96

Leu Val Pro Val Ala Glu Cys lie Asn Ser Ala lie Ser Asn Gly Ser -181 - 136852-序列表.doc i si 1441334884

20 25 30 Cct gat tea aca tee aca tea gaa caa gtt gaa gag gaa cct ttc ett Ser Asp Ser Thr Ser Thr Ser Glu Gin Val Glu Glu Glu Pro Phe Leu 35 40 45 eta gaa caa tat tea ett tee tee gtg tet tta tta gta aaa age aeg Leu Glu Gin Tyr Ser Leu Ser Ser Val Ser Leu Leu Val Lys Ser Thr 50 55 60 ttc aat cct get ccg tat gca atg caa ttg act tgg aaa cag ett tet Phe Asn Pro Val Ser Tyr Ala Met Gin Leu Thr Trp Lys Gin Leu Ser 6S 70 75 80 att tta ttt tta act gtg att tet gtt cct gtt ttg get gag gga aaa lie Leu Phe Leu Thr Val lie Ser Val Pro Val Leu Ala Glu Gly Lys 85 90 95 999 gaa aga aat caa tta aca gtg att: gat aat age gat cat att Gly Asp Glu Arg Asn Gin Leu Thr Val lie Asp Asn Ser Asp His lie 100 105 110 aaa tta gat gca tet aat etc get ggt aat gat aaa aca aaa ate tat Lys Leu Asp Ala Ser Asn Leu Ala Gly Asn Asp Lys Thr Lys He Tyr 115 X20 125 caa gca gaa aat aaa gtt ctg gtt att gat att get aaa cca aat ggg Gin Ala Glu Asn Lys Val Leu Val lie Asp lie Ala Lys Pro Asn Gly 130 135 X40 aaa ggg att tea gat aac cgt ttt gaa aaa ttt aat att cca aat age Lys Gly lie Ser Asp Asn Arg Phe Glu Lys Phe Asn lie Pro Asn Ser 145 150 155 160 geg gtg ttt aat aat aat ggg act gaa geg cag gca aga tea aca tta Ala Val Phe Asn Asn Asn Gly Thr Glu Ala Gin Ala Arg Ser Thr Leu 165 170 175 att ggt tac att ccg caa aat caa aat t:ta agg gga ggg aaa gaa get lie Gly Tyr lie Pro Gin Asn Gin Asn Leu Arg Gly Gly Lys Glu Ala 180 185 190 gat gtt ata tta aat caa gtg aca ggt cct caa gaa agt aaa att gtt Asp Val lie Leu Asn Gin Val Thr Gly Pro Gin Glu Ser Lys lie Val 195 200 205 ggc geg ett gaa gta tta ggt aaa aaa get gat ate gtc att gca aac Gly Ala Leu Glu Val Leu Gly Lys Lys Ala Asp lie Val He Ala Asn 210 215 220 caa aat ggt att acc tta aat ggt gta aga aca ata aat tea gat cgt Gin Asn Gly lie Thr Leu Asn Gly Val Arg Thr lie Asn Ser Asp Arg 225 230 235 240 ttt gtt gee act aeg agt gag ett ata gat ccg aat cag atg atg tta Phe Val Ala Thr Thr Ser Glu Leu lie Asp Pro Asn Gin Met Met Leu 245 250 255 aag gtt aca aaa gga aat gtg ate att gat att gat ggt cct teg aca Lys Val Thr Lys Gly Asn Val lie lie Asp lie Asp Gly Phe Ser Thr 260 265 270 gat gga tta aag tat tta gat att att get aaa aaa att gaa caa aag 192 240 288 336 384 432 480 528 576 624 672 720 768 Θ16 864 136852·序列表.doc -182- is] 1334884

Asp Gly Leu 275 Lys Tyr Leu Asp lie 280 lie Ala Lys Lys lie 285 Glu Gin Lys caa tea att aca tea ggg gat aat tea gaa gca aaa aca gat gtc act 912 Gin Ser 290 He Thr Ser Gly Asp 295 Asn Ser Glu Ala Lys 300 Thr Asp Val Thr ctt att geg ggt tcc agt gaa tat gat tta age aaa cat gag ctg aaa 960 Leu 305 lie Ala Gly Ser Ser 310 Glu Tyr Asp Leu Ser 315 Lys His Glu Leu Lys 320 aaa aeg age ggt gaa aat gta tct aat gat gtt att get ate aeg gga 1008 Lys Thr Ser Gly Glu 325 Asn Val Ser Asn Asp 330 Val He Ala lie Thr Gly 33S tct agt aca ggc gca atg cat ggt aaa aat att aag ttg att gtg aca 1056 Ser Ser Thr Gly Ala 340 Met His Gly Lys 345 Asn lie Lys Leu lie 350 val Thr gat aaa ggt gca ggc gta aaa cat gat gga att att ttg tct gaa aat 1104 Asp Lys Gly Ala 355 Gly Val Lys His 360 Asp Gly lie lie Leu 365 Ser Glu Asn gat att cag att gaa atg aat gaa ggt gac tta gaa ctt ggc aat aeg 1152 Asp lie 370 Gin lie Glu Met Asn 37S Glu Gly Asp Leu Glu 380 Leu Gly Asn Thr att cag caa aca gtg gta aaa aaa gac ega aat att ega gcc aag aaa 1200 lie 385 Gin Gin Thr Val Val 390 Lys Lys Asp Arg Asn 395 lie Arg Ala Lys Lys 400 aaa att gaa gtg aaa aac get aat cgt gtt ttt gtt ggt agt caa aeg 1248 Lys lie Glu Val Lys 405 Asn Ala Asn Arg Val 410 Phe Val Gly Ser Gin 415 Thr aaa tea gat gaa att teg tta gag geg aaa caa gtt aaa ate aga aaa 1296 Lys Ser Asp Glu 420 lie Ser Leu Glu Ala 425 Lys Gin Val Lys lie 430 Arg Lys aac gca gag att agg agt aeg aca caa gcc aaa ate gta gca aag ggt 1344 Asn Ala Glu 435 He Arg Ser Thr Thr 440 Gin Ala Lys lie Val 445 Ala Lys Gly gcc ctg tct att gag caa aat geg aag etc gtc get aaa aag ata gat 1392 Ala Leu 450 Ser lie Glu Gin Asn 455 Ala Lys Leu Val Ala 460 Lys Lys lie Asp gtg gca aca gaa act eta act aat get ggg cgt att tat ggt ega 9^9 1440 Val 465 Ala Thr Glu Thr Leu 470 Thr Asn Ala Gly Arg 475 lie Tyr Gly Arg Glu 480 gtt aag ctt gac act aat aat ttg att aat gat aaa gaa att tat get 1488 Val Lys Leu Asp Thr 485 Asn Asn Leu lie Asn 490 Asp Lys Glu lie Tyr 495 Ala gaa egg aaa ttg agt att ttg aeg aaa gga aaa gat ctt gaa att att 1536 Glu Arg Lys Leu 500 Ser lie Leu Thr Lys 505 Gly Lys Asp Leu Glu 510 lie lie caa gat aga tat ttg tct cca ctg atg ege gta aaa agt agt gtc ege 1584 Gin Asp Arg 515 Tyr Leu Ser Pro Leu 520 Met Arg Val Lys Ser 525 Ser Val Arg 136852·序列表.doc -183- l Si 1334884

ttt tta ggc tet ccg ttt ttc tea ata tet ccg teg atg etc gca age 1632 Phe Leu 530 Gly Ser Pro Phe Phe 535 Ser lie Ser Pro Ser 540 Met Leu Ala Ser ctt agt gca cag ttt aag cct ggt ttt gtg aat aag gga etc att gaa 1680 Leu 545 Ser Ala Gin Phe Lys 550 Pro Gly Phe Val Asn 555 Lys Gly Leu lie Glu 560 agt geg ggg agt gca gaa tta act ttt aaa gaa aaa acc agt ttt tta 1728 Ser Ala Gly Ser Ala 565 Glu Leu Thr Phe Lys 570 Glu Lys Thr Ser Phe 575 Leu aca gag ggc aat aat ttt att aga get aaa gat geg tta act att aac 1776 Thr Glu Gly Asn 580 Asn Phe lie Arg Ala 585 Lys Asp Ala Leu Thr 590 lie Asn gcc caa aat att gaa att gat aaa aat caa gat att caa ttg ggt get 1824 Ala Gin Asn 595 lie Glu lie Asp Lys 600 Asn Gin Asp lie Gin 605 Leu Gly Ala aat ata aeg ttg aat gtg gaa gaa aac ttt gtt aat cgt gca gga aca 1872 Asn lie 610 Thr Leu Αεη Val Glu 615 Glu Asn Phe Val Asn 620 Arg Ala Gly Thr ctg gca act ggt aaa aca ctg aca att aat acc gaa agt ggc agt att 1920 Leu 625 Ala Thr Gly Lys Thr 630 Leu Thr lie Asn Thr 635 Glu Ser Gly Ser lie 64 0 tac aat ctt ggt ggg aca tta ggt get gga aaa tea tta aaa ctg act 1968 Tyr Asn Leu Gly Gly Thr 645 Leu Gly Ala Gly Lys 650 Ser Leu Lys Leu 655 Thr get aaa tea aeg gaa gaa ggt atg gga aat att gtt aac caa gaa aac 2016 Ala Lys Ser Thr 660 Glu Glu Gly Met Gly 665 Asn lie Val Asn Gin 670 Glu Asn ggt tta ttc cat aca etc ggt aat atg atg tta gaa gca gag cgt tet 2064 Gly Leu Phe 675 His Thr Leu Gly Asn 680 Met Met Leu Glu Ala 685 Glu Arg Ser gtt tat aat att ggc gat att tat geg agt aaa aaa tta aca gtt cat 2112 Val Tyr 690 Asn lie Gly Asp lie 695 Tyr Ala Ser Lys Lys 700 Leu Thr Val His act cat aat ttg att aat gat gtg cgt tta tet ggc aat gtg agt tat 2160 Thr 70S His Asn Leu lie Asn 710 Asp Val Arg Leu Ser 715 Gly Asn Val Ser Tyr 720 aag cct ate ggt tea agt cgt gat tat gat ate agt cgt gtt geg gta 2208 Lys Pro lie Gly Ser 725 Ser Arg Asp Tyr Asp 730 lie Ser Arg Val Ala 735 Val cat ggt tgg cac aat aat gtt tat aag etc aac tta aat ctg caa gaa 2256 His Gly Trp His 740 Asn Asn Val Tyr Lys 745 Leu Asn Leu Asn Leu 750 Gin Glu caa gat aaa acc gat att aaa gtt gtg aaa atg ggg get ate cgt tet 2304 Gin Asp Lys 755 Thr Asp lie Lys Val 760 Val Lys Met Gly Ala 765 lie Arg Ser gat ggt gat ttt gac ttt aag gga ata aag geg aca tea tea gaa tea 2352 Asp Gly 770 Asp Phe Asp Phe Lys 775 Gly lie Lys Ala Thr 780 Ser Ser Glu Ser 136852-序列表.doc •184· 151 1334884

aaa ccg cag tta att aat cat gga tta att aat gtc aaa gga aca ttt 2400 Lys 785 Pro Gin Leu lie Asn 790 His Gly Leu lie Asn Val Lys Gly Thr Phe 795 800 aac geg gaa get gat caa gtg gtg aac caa atg aaa geg ttt aac caa 2448 Asn Ala Glu Ala Asp 805 Gin Val Val Asn Gin 810 Met Lys Ala Phe Asn Gin 815 aat gca tta gca age gtg ttt aag aat cca geg aaa ate aeg atg tac 2496 Asn Ala Leu Ala 820 Ser Val Phe Lys Asn Pro 825 Ala Lys lie Thr Met Tyr Θ30 tat caa cca ett act cgt tat att tgg aca cca tta teg ggt aat gca 2544 Tyr Gin Pro 835 Leu Thr Arg Tyr lie 840 Trp Thr Pro Leu Ser Gly Asn Ala 845 teg cgt gaa ttt aac aat tta gag tet ttc etc gat gee ttg ttt ggc 2592 Ser Arg Glu 850 Phe Asn Asn Leu 855 Glu Ser Phe Leu Asp Ala Leu Phe Gly 860 tea aca aca ate tta aaa tea agt ttc tat agt aeg gaa aat ttt agt 2640 Ser 865 Thr Thr lie Leu Lys 870 Ser Ser Phe Tyr Ser Thr Glu Λεη Phe Ser 075 880 get tat cag ett eta tet cat att cag cat tea cca atg tac caa aaa 2688 Ala Tyr Gin Leu Leu 885 Ser His lie Gin His 890 Ser Pro Met Tyr Gin Lys 895 geg atg gca caa gtg ttt ggt gca gag tgg cat agt aaa tee tat gat 2736 Ala Met Ala Gin 900 Val Phe Gly Ala Glu Trp 905 His Ser Lys Ser Tyr Asp 910 gag atg ega aac aaa tgg aaa age ttt aaa gaa aat cca aca gat ttc 2784 Glu Met Arg 915 Asn Lys Trp Lys Ser 920 Phe Lys Glu Asn Pro Thr Asp Phe 925 att tat tac cca tea gaa aaa gca aaa ate eta geg gga aaa eta gaa 2832 lie Tyr 930 Tyr Pro Ser Glu Lys 935 Ala Lys lie Leu Ala Gly Lys Leu Glu 940 ggt aag ett aca aeg eta caa aat ggt gaa tat gee gaa cgt ggt aag 2880 Gly Lys 945 Leu Thr Thr Leu 950 Gin Asn Gly Glu Tyr Ala Glu Arg Gly Lys 955 960 ttt gat gag agt ate caa att ggt aaa cac caa tta teg eta cca tea 2928 Phe Asp Glu Ser lie 965 Gin lie Gly Lys His 970 Gin Leu Ser Leu Pro Ser 975 gta gag ett aaa geg gag ttt agt gat aaa gaa cgt ttg gaa gag gac 2976 Val Glu Leu Lys 980 Ala Glu Phe Ser Asp Lys 985 Glu Arg Leu Glu Glu Asp 990 ggg gta gat tta tee teg ate gee gaa etc tta gaa atg cca aac tta 3024 Gly Val Asp 995 Leu Ser Ser lie Ala 1000 Glu Leu Leu Glu Met Pro Asn Leu 1005 ttt att gat aat agt ate caa tta gaa aag aaa aag ttg tet cct att 3072 Phe He 1010 Asp Asn Ser lie Gin Leu Glu Lys Lys Lys Leu Ser Pro lie 1015 1020 gag gat eta gat gaa gaa cca cgt aaa aat ctg gat ata gaa gaa age 3120 Glu 1025 Asp Leu Asp Glu Glu Pro Arg Lys Asn Leu Asp lie Glu Glu Ser 1030 1035 1040 136852-序列表.doc -185-

l SI 1334884 cat tct aat tea teg gat gac gtg ett age atg aat gat gat gag tet 3168

His Ser Asn Ser Ser Asp Asp Val Leu Ser Met Asn Asp Asp Glu Ser 1045 1050 1055 gat aca gac gat age aag tgg agt atg ggc aat gat gag aaa gag atg 3216

Asp Thr Asp Asp Ser Lys Trp Ser Met Gly Asn Asp Glu Lys Glu Met 1060 1065 1070 ccc gat gat aag ctg ggt ata agt cgt gat gat cgt gga aat aaa cca 3264

Pro Asp Asp Lys Leu Gly He Ser Arg Asp Asp Arg Gly Asn Lys Pro 1075 1080 1085 cct cgt act gat cct aca gtt gat tat ett aac cct gat gaa ttc ttt 3312

Pro Arg Thr Asp Pro Thr Val Asp Tyr Leu Asn Pro Asp Glu Phe Phe 1090 1095 1100

gaa aat ggt tat etc ttg aat gag eta eta cag gag ett gga gaa gag 3360 Glu Asn Gly Tyr Leu Leu Asn Glu Leu Leu Gin Glu Leu Gly Glu Glu 1105 1110 1115 1120 ccg tta eta aaa gaa ggg gaa gat cat ttt aaa cgt tct acc aat eta 3408 Pro Leu Leu Lys Glu Gly Glu Asp His Phe Lys Arg Ser Thr Asn Leu 1125 1130 1135 gtc cgt eta ggc gag aga gat agg caa aat aga gaa aag aga gaa aaa 3456 Val Arg Leu Gly Glu Arg Asp Arg Gin Asn Arg Glu Lys Arg Glu Lys 1140 1145 1150 gag ggg tat ttt gat ctg cct ggt aca tta gat atg aaa ctg cag gag 3504 Glu Gly Tyr Phe Asp Leu Pro Gly Thr Leu Asp Met Lys Leu Gin Glu 1155 1160 1165 tta ttc gaa aaa aga aaa caa aaa cac gaa gca gaa cag aaa gca aga 3552 Leu Phe Glu Lys Arg Lys Gin Lys His Glu Ala Glu Gin Lys Ala Arg 1170 1175 1180 ata gaa aaa gca ett eta caa [aaa tea gaa caa caa l gaa aaa cgt gtt 3600 lie Glu l Lys Ala Leu Leu Gin t Lys Ser Glu Gin Gin Glu Lys Arg Val 1185 1190 1195 1200 gaa gaa cgt aag caa gag gaa aaa cgt caa geg caa gat aaa att get 364Θ Glu Glu Arg Lys丨 Gin Glu Glu Lys Arg i Gin Ala Gin Asp Lys lie Ala 1205 1210 1215 aag caa gta gaa att gca aaa gaa atg caa egg gta gaa gaa att ege 3696 Lys Gin Val Glu lie Ala Lys Glu Met Gin Arg Val Glu Glu lie Arg 1220 1225 1230 cag aga gaa aaa caa ett geg ate caa ctg caa gaa gaa gag aag aaa 3744 Gin Arg Glu Lys Gin Leu Ala lie Gin Leu Gin Glu Glu Glu Lys Lys 1235 1240 1245 caa caa gaa gaa aaa cat tta tee gag gag aaa aaa caa get gaa cag 3792 Gin Gin Glu Glu Lys His Leu Ser Glu Glu Lys Lys Gin Ala Glu Gin 1250 1255 1260 aaa caa aaa get gag gag aaa gtt gca caa gaa aga tta gac att gaa 3840 Lys Gin hys Ala Glu Glu Lys Val Ala Gin Glu Arg Leu Asp lie Glu 1265 1270 1275 1280 caa cag aaa geg tat gaa gaa atg geg , aag ega gag gca gag < 3ca tea 3888 Gin Gin Lys Ala _ Tyr Glu Glu Met Ala Lys Arg Glu Ala Glu , ^la Ser 1285 1290 1295 -186- 136852·序歹丨j表.doc i S3 1334884 aaa aat gtt tta ttg aaa gcg att gat gaa gaa cgt cca aaa gtg gaa 3936

Lys Asn Val Leu Leu Lys Ala He Asp Glu Glu Arg Pro Lys Val Glu 1300 1305 1310 act gat cca ctt ttc cgt aca aaa ttg aaa tat ate aat caa gat gac 3984

Thr Asp Pro Leu Phe Arg Thr Lys Leu Lys Tyr lie Asn Gin Asp Asp 1315 1320 1325 tat get ggt gca aat tat ttc ttc aat aaa gtt ggt tta aat aca aaa 4032

Tyr Ala Gly Ala Asn Tyr Phe Phe Asn Lys Val Gly Leu Asn Thr Lys 1330 1335 1340 ggt cat caa aaa gta aat gtg tta ggg gat aac tat ttt gat cat caa 4080 Gly His Gin Lys Val Asn Val Leu Gly Asp Asn Tyr Phe Asp His Gin 1345 1350 1355 1360 gtg att act ege teg att gag aaa aaa gta gat aac cac ctt aac caa 4128

Val lie Thr Arg Ser lie Glu Lys Lys Val Asp Asn His Leu Asn Gin 1365 1370 1375 aaa tac aat etc age gat gtg gaa tta gtt aaa cag ctg atg gac aat 4176

Lys Tyr Asn Leu Ser Asp Val Glu Leu Val Lys Gin Leu Met Asp Asn 1380 1385 1390 tcc aca aca caa gcg cag gag ttg gat ttg aaa eta ggt gcg gca tta 4224

Ser Thr Thr Gin Ala Gin Glu Leu Asp Leu Lys Leu Gly Ala Ala Ltu 1395 1400 1405 act aaa gaa caa caa get aac ttg acc caa gat ate gtt tgg tat gtc 4272

Thr Lys Glu Gin Gin Ala Asn Leu Thr Gin Asp He Val Trp Tyr Val 1410 1415 1420 aaa aeg aag gta aag ggc aaa gat gtg ttt gtt cca aag gtt tat ttc 4320 Lys Thr Lys Val Lys Gly Lys Asp Val Phe Val Pro Lys Val Tyr Phe 1425 1430 1435 1440 get tet gaa aeg etc gta gaa gee caa aaa tta caa ggt tta ggc act 4368

Ala Ser Glu Thr Leu Val Glu Ala Gin Lys Leu Gin Gly Leu Gly Thr 1445 1450 1455 ggg act ate aga gtt ggt gaa get aag att aaa gee aaa gat gtg gtg 4416

Gly Thr lie Arg Val Gly Glu Ala Lys lie Lys Ala Lys Asp Val Val 1460 1465 1470

aat acc ggg aca tta get ggg aga aaa etc aat gtt gaa gcg agt aat 4464

Asn Thr Gly Thr Leu Ala Gly Arg Lys Leu Asn Val Glu Ala Ser Asn 1475 1480 1485 aaa ate aaa aat caa ggg agt ate tta agt act caa gaa aca cgt tta 4512 Lys lie Lys Asn Gin Gly Ser lie Leu Ser Thr Gin Glu Thr Arg Leu 1490 1495 1500 gtc ggg cgt aaa ggt att gaa aac gta tet cgt tea ttt gca aat gat 4560 Val Gly Arg Lys Gly lie Glu Asn Val Ser Arg Ser Phe Ala Asn Asp 1505 1510 1515 1520 gaa tta gga gtc act gca caa ege tea gaa ate aaa aeg gaa ggt cat 4608

Glu Leu Gly Val Thr Ala Gin Arg Ser Glu lie Lys Thr Glu Gly His 1525 1530 1535 tta cat ctt gaa aca gat aag gat tea act att gat gta caa gca teg 4656

Leu His Leu Glu Thr Asp Lys Asp Ser Thr lie Asp Val Gin Ala Ser 1540 1545 1550 •187· 136852·序列表.doc 1334884 gat att aaa gca aaa aca age ttt gtg aag act ggt gat gtg aat etc 4704

Asp lie Lys Ala L»ys Thr Ser Phe Val Lys Thr Gly Asp Val Asn Leu 1555 1560 * 1565 aaa aat aca tac aat act aaa cat gec tac cgt gag aaa ttc teg ccg 4752

Lys Asn Thr Tyr Asn Thr Lys His Ala Tyr Arg Glu Lys Phe Ser Pro 1570 1575 1580 agt gca eta caa get gca gaa ett gat gtg gca ggg ett aaa gtc cca 4800

Ser Ala Leu Gin Val Ala Glu Leu Asp Val Ala Gly Leu Lys Val Pro 15bi 1590 1595 1600 ett tta ggc gtg tee gtc tee ate cag ttt att cag age ata eta gtg 4848

Leu Leu Gly Val Ser Val Ser lie Gin Phe lie Gin Ser lie Leu Val 1605 1610 1615 agg caa ett caa gag gga tea ate ttc gaa gta ggg cac tta cat ntt 4896

Arg Gin Leu Gin Glu Gly Ser lie Phe Glu Val Gly His Leu His Xaa 1620 1625 1630 geg gta gac aga aga tgt gaa cca age ggg gag ta 4931

Ala Val Asp Arg Arg Cys Glu Pro Ser Gly Glu 1635 1640 <210> 103 <211> 1643 <212>私白前 <213> WuH巴肋德本 <220> <221> imsc <222> \〇}2 <223> X抑=任知的將拖故 <400> 103

Met Asn Lys Asn Arg Tyr Lys Leu He Phe Ser Gin Val Lys Gly Cys 1 5 10 15

Leu Val Pro Val Ala Glu Cys lie Asn Ser Ala lie Ser Asn Gly Ser 20 25 30

Ser Asp Ser Thr Ser Thr Ser Glu Gin Val Glu Glu Glu Pro Phe Leu 35 40 45

Leu Glu Gin Tyr Ser Leu Ser Ser Val Ser Leu Leu Val Lys Ser Thr 50 55 60

Gly Asp Glu Arg Asn Gin Leu Thr Val lie Asp Asn Ser Asp His lie 100 105 110

Lys Leu Asp Ala Ser Asn Ueu Ala Gly Asn Asp Lys Thr Lys He Tyr 115 120 125

Gin Ala Glu Asn Lys Val Leu Val lie Asp lie Ala Lys Pro Asn Gly 130 135 140 -188· 136852-序列表.doc T \1 i. Λ 1334884

Lys Gly lie Ser Asp Asn Arg Phe Glu Lys Phe Asn lie Pro Asn Ser 145 150 155 160

Asp Val lie Leu Asn Gin Val Thr Gly Pro Gin Glu Ser Lys lie Val 195 200 205

Gly Ala Leu Glu Val Leu Gly Lys bys Ala Asp lie Val lie Ala Asn 210 215 220

Gin Asn Gly lie Thr Leu Asn Gly Val Arg Thr lie Asn Ser Asp Arg 225 230 235 240

Phe Val Ala Thr Thr Ser Glu Leu lie Asp Pro Asn Gin Met Met Leu 245 250 255

Lys Val Thr Lys Gly Asn Val lie lie Asp He Asp Gly Phe Ser Thr 260 265 270

Asp Gly Leu Lys Tyr Leu Asp lie lie Ala Lys Lys lie Glu Gin Lys 275 280 285

Gin Ser lie Thr Ser Gly Asp Asn Ser Glu Ala Lys Thr Asp Val Thr 290 295 300

Leu lie Ala Gly Ser Ser Glu Tyr Asp Leu Ser Lys His Glu Leu Lys 305 310 315 320

Lys Thr Ser Gly Glu Asn Val Ser Asn Asp Val lie Ala lie Thr Gly 325 330 335

Ser Ser Thr Gly Ala Met His Gly Lys Asn lie Lys Leu lie Val Thr 340 345 350

Asp Lys Gly Ala Gly Val Lys His Asp Gly lie lie Leu Ser Glu Asn 355 360 365

Asp lie Gin lie Glu Met Asn Glu Gly Asp Leu Glu Leu Gly Asn Thr 370 375 380 lie Gin Gin Thr Val Val Lys Lys Asp Arg Asn lie Arg Ala Lys Lys 385 390 395 400

Lys lie Glu Val Lys Asn Ala Asn Arg Val Phe Val Gly Ser Gin Thr 405 410 415

Lys Ser Asp Glu lie Ser Leu Glu Ala Lys Gin Val Lys lie Arg Lys 420 425 430

Asn Ala Glu lie Arg Ser Thr Thr Gin Ala Lys lie Val Ala Lys Gly 435 440 445

Ala Leu Ser lie Glu Gin Asn Ala Lys Leu Val Ala Lys Lys lie Asp 450 455 460

Val Ala Thr Glu Thr Leu Thr Asn Ala Gly Arg lie Tyr Gly Arg Glu 465 470 475 480 •189· 136852·序列表.doc 1334884

Val Lys Leu Asp Thr Asn Asn Leu lie Asn Asp Lys Glu lie Tyr Ala 485 490 495

Glu Arg Lys Leu Ser lie Leu Thr Lys Gly Lys Asp Leu Glu lie lie 500 505 510

Gin Asp Arg Tyr Leu Ser Pro Leu Met Arg Val Lys Ser Ser Val Arg 515 520 525

Phe Leu Gly Ser Pro Phe Phe Ser lie Ser Pro Ser Met Leu Ala Ser 530 535 540

Leu Ser Ala Gin Phe Lys Pro Gly Phe Val Asn Lys Gly Leu lie Glu 545 550 555 560

Ser Ala Gly Ser Ala Glu Leu Thr Phe Lys Glu Lys Thr Ser Phe Leu 565 570 575

Thr Glu Gly Asn Asn Phe lie Arg Ala Lys Asp Ala Leu Thr lie Asn 580 585 590

Ala Gin Asn lie Glu lie Asp Lys Asn Gin Asp lie Gin Leu Gly Ala 595 600 605

Asn He Thr Leu Asn Val Glu Glu Asn Phe Val Asn Arg Ala Gly Thr 610 615 620

Leu Ala Thr Gly Lys Thr Leu Thr lie Asn Thr Glu Ser Gly Ser lie 625 630 635 640

Tyr Asn Leu Gly Gly Thr Leu Gly Ala Gly Lys Ser Leu Lys Leu Thr 645 €50 655

Ala Lys Ser Thr Glu Glu Gly Met Gly Asn lie Val Asn Gin Glu Asn 660 665 670

Gly Leu Phe His Thr Leu Gly Asn Met Met Leu Glu Ala Glu Arg Ser 675 680 685

Val Tyr Asn lie Gly Asp He Tyr Ala Ser Lys Lys Leu Thr Val His 690 695 700

Thr His Asn Leu lie Asn Asp Val Arg Leu Ser Gly Asn Val Ser Tyr 705 710 715 720

Lys Pro lie Gly Ser Ser Arg Asp Tyr Asp lie Ser Arg Val Ala Val 725 730 735

His Gly Trp His Asn Asn Val Tyr Lys Leu Asn Leu Asn Leu Gin Glu 740 745 750

Gin Asp Lys Thr Asp He Lys Val Val Lys Met Gly Ala He Arg Ser 755 760 765

Asp Gly Asp Phe Asp Phe Lys Gly He Lys Ala Thr Ser Ser Glu Ser 77〇 775 780

Lys Pro Gin Leu lie Asn His Gly Leu lie Asn Val Lys Gly Thr Phe 785 790 795 800

Asn Ala Glu Ala Asp Gin Val Val Asn Gin Met Lys Ala Phe Asn Gin 805 810 815 •190- 136852·序列表.doc

1 SI 1334884

Asn Ala Leu Ala Ser Val Phe Lys Asn Pro Ala Lys lie Thr Met Tyr 820 825 Θ30

Tyr Gin Pro Leu Thr Arg Tyr lie Trp Thr Pro Leu Ser Gly Asn Ala 835 640 845

Ser Arg Glu Phe Asn Asn Leu Glu Ser Phe Leu Asp Ala Leu Phe Gly 850 655 860

Ser Thr Thr lie Leu Lys Ser Ser Phe Tyr Ser Thr Glu Asn Phe Ser 865 870 875 880

Ala Tyr Gin Leu Leu Ser His lie Gin His Ser Pro Met Tyr Gin Lys 885 890 895

Ala Met Ala Gin Val Phe Gly Ala Glu Trp His Ser Lys Ser Tyr Asp 900 905 910

Glu Met Arg Asn Lys Trp Lys Ser Phe Lys Glu Asn Pro Thr Asp Phe 915 920 925

lie Tyr Tyr Pro Ser Glu Lys Ala Lys lie Leu Ala Gly Lys lieu Glu 930 935 940

Gly Lys Leu Thr Thr Leu Gin Asn Gly Glu Tyr Ala Glu Arg Gly Lys 945 950 955 960

Phe Asp Glu Ser lie Gin lie Gly Lys His Gin Leu Ser Leu Pro Ser 965 970 975

Val Glu Leu Lys Ala Glu Phe Ser Asp Lys Glu Arg Leu Glu Glu Asp 980 985 990

Gly Val Asp Leu Ser Ser lie Ala Glu Leu Leu Glu Met Pro Asn Leu 995 1000 1005

Phe lie Asp Asn Ser lie Gin Leu Glu Lys Lys Lys Leu Ser Pro lie 1010 1015 1020

Glu Asp Leu Asp Glu Glu Pro Arg Lys Asn Leu Asp lie Glu Glu Ser 1025 1030 1035 1040

His Ser Asn Ser Ser Asp Asp Val Leu Ser Met Asn Asp Asp Glu Ser

1045 1050 1055

Asp Thr Asp Asp Ser Lys Trp Ser Met Gly Asn Asp Glu Lys Glu Met 1060 1065 1070

Pro Asp Asp Lys Leu Gly lie Ser Arg Asp Asp Arg Gly Asn Lys Pro 1075 1080 1085

Pro Arg Thr Asp Pro Thr Val Asp Tyr Leu Asn Pro Asp Glu Phe Phe 1090 1095 1100

Glu Asn Gly Tyr Leu Leu Asn Glu Leu Leu Gin Glu Leu Gly Glu Glu 1105 1110 1115 1120

Pro Leu Leu Lys Glu Gly Glu Asp His Phe Lys Arg Ser Thr Asn Leu 1125 1130 1135

Val Arg Leu Gly Glu Arg Asp Arg Gin Asn Arg Glu Lys Arg Glu Lys 1140 1145 1150 191- 136852-序列表.doc 1334884

Glu Gly Tyr Phe Asp Leu Pro Gly Thr Leu Asp Met Lys Leu Gin Glu 1155 1160 1165

Leu Phe Glu Lys Arg Lys Gin Lys His Glu Ala Glu Gin Lys Ala Arg 1170 1175 1180 lie Glu Lys Ala Leu Leu Gin Lys Ser Glu Gin Gin Glu Lys Arg Val 1185 1190 1X95 1200

Glu Glu Arg Lys Gin Glu Glu Lys Arg Gin Ala Gin Asp Lys lie Ala 1205 1210 1215

Lys Gin Val Glu lie Ala Lys Glu Met Gin Arg Val Glu Glu lie Arg 1220 1225 1230

Gin Arg Glu Lys Gin Leu Ala lie Gin Leu Gin Glu Glu Glu Lys Lys 1235 1240 X245

Gin Gin Glu Glu Lys His Leu Ser Glu Glu Lys Lys Gin Ala Glu Gin 1250 1255 1260

Lys Gin Lys Ala Glu Glu Lys Val Ala Gin Glu Arg Leu Asp lie Glu 1265 1270 1275 1280

Gin Gin Lys Ala Tyr Glu Glu Met Ala Lys Arg Glu Ala Glu Ala Ser 1285 1290 1295

Lys Asn Val Leu Leu Lys Ala lie Asp Glu Glu Arg Pro Lys Val Glu 1300 1305 1310

Thr Asp Pro Leu Phe Arg Thr Lys Leu Lys Tyr lie Asn Gin Asp Asp 1315 1320 1325

Tyr Ala Gly Ala Asn Tyr Phe Phe Asn Lys Val Gly Leu Asn Thr Lys 1330 1335 1340

Gly His Gin Lys Val Asn Val Leu Gly Asp Asn Tyr Phe Asp His· Gin 1345 1350 1355 1360

Val lie Thr Arg Ser He Glu Lys Lys Val Asp Asn His Leu Asn Gin 1365 1370 1375

Lys Tyr Asn Leu Ser Asp Val Glu Leu Val Lys Gin Leu Met Asp Asn 1380 1385 1390

Ser Thr Thr Gin Ala Gin Glu Leu Asp Leu Lys Leu Gly Ala Ala Leu 1395 1400 1405

Thr Lys Glu Gin Gin Ala Asn Leu Thr Gin Asp lie Val Trp Tyr Val 1410 1415 1420

Lys Thr Lys Val Lys Gly Lys Asp Val Phe Val Pro Lys Val Tyr Phe 1425 1430 1435 1440

Ala Ser Glu Thr Leu Val Glu Ala Gin Lys Leu Gin Gly Leu Gly Thr 1445 1450 1455

Gly Thr lie Arg Val Gly Glu Ala Lys lie Lys Ala Lys Asp Val Val 1460 1465 1470

Asn Thr Gly Thr Leu Ala Gly Arg Lys Leu Asn Val Glu Ala Ser Asn 1475 1480 1485 -192- 136852-序列表.doc

l SI 1334884

Lys lie Lys Asn Gin Gly Ser lie Leu Ser Thr Gin Glu Thr Arg Leu 1490 1495 1500

Val Gly Arg Lys Gly lie Glu Asn Val Ser Arg Ser Phe Ala Asn Asp 1505 15X0 1515 1520

Glu Leu Gly Val Thr Ala Gin Arg Ser Glu lie Lys Thr Glu Gly His 1525 1530 1535

Leu His Leu Glu Thr Asp Lys Asp Ser Thr lie Asp Val Gin Ala Ser 1540 1545 1550

Asp lie Lys Ala Lys Thr Ser Phe Val Lys Thr Gly Asp Val Asn Leu 1555 1560 1565

Lys Asn Thr Tyr Asn Thr Lys His Ala Tyr Arg Glu Lys Phe Ser Pro 1570 1575 1580

Ser Ala Leu Gin Val Ala Glu Leu Asp Val Ala Gly Leu Lys Val Pro 1585 1590 1595 1600

Leu Leu Gly Val Ser Val Ser lie Gin Phe lie Gin Ser lie Leu Val 1605 1610 1615

Arg Gin Leu Gin Glu Gly Ser lie Phe Glu Val Gly His Leu His Xaa 1620 1625 1630

Ala Val Asp Arg Arg Cys Glu Pro Ser Gly Glu 1635 1640

<210> 104 <211> 2009 <212> DNA <213>收血性巴斯洗桴胡 <220>

<223> hmbR <220>

<221> CDS <222> (1) . . (2007)

<400> 104 ate cgt ggc get gat aaa gat cgt gtc get gtt att gtt gat gga ata 48 lie Arg Gly Val Asp Lys Asp Arg Val Ala Val lie Val Asp Gly lie 15 l〇 15 ccg cag get gaa teg act ata tet act tcc gca cgt tat teg act gaa 96

Pro Gin Ala Glu Ser Thr He Ser Thr Ser Ala Arg Tyr Ser Thr Glu 20 25 30 cgt cat aat ggt aat att aat aat att gaa tac gaa aat gtt agt teg 144

Arg His Asn Gly Asn He Asn Asn He Glu Tyr Glu Asn Val Ser Ser 35 40 45 ttg aaa gtt caa aaa ggg gca get tet gta atg tat ggt age ggt geg 192

Leu Lys Val Gin Lys Gly Ala Ala Ser Val Met Tyr Gly Ser Gly Ala 50 55 60 tta ggt gga acc gtg gag ttt acc aca aaa gat att gag gac ttt gtc 240

Leu Gly Gly Thr Val Glu Phe Thr Thr Lys Asp He Glu Asp Phe Val 65 70 75 80 •193- 136852-序列表.doc 1334884

gaa cct ggt ege cat ttg ggc ttt ttg tet aaa acc ggc tat act tea 288 Glu Pro Gly Arg His 85 Leu Gly Phe Leu Ser 90 Lys Thr Gly Tyr Thr 95 Ser aaa aac aga gaa tat cgt caa gtc ate gga gtt gga ggg aaa ggg gaa 336 Lys Asn Arg Glu 100 Tyr Arg Gin Val lie 105 Gly Val Gly Gly Lys 110 Gly Glu cac ttt ttt ggt ttt gta caa tta acc aaa cgt tgg ggg cat gaa aca 384 His Phe Phe 115 Gly Phe Val Gin Leu 120 Thr Lys Arg Trp Gly His 125 Glu Thr ate aac aac ggc aaa ggt aca gac att etc ggc gaa cat ega ggt aaa 432 lie Asn 130 Asn Gly Lys Gly Thr Asp 135 He Leu Gly Glu 140 His Arg Gly Lys ccc aat ccg etc aac tac tat act aca tea tgg tta aeg aaa gtc ggt 480 Pro 145 Asn Pro Leu Asn Tyr 150 Tyr Thr Thr Ser Trp 155 Leu Thr Lys Val Gly 160 tac gat att aat aac act cat cgt ttt aca ctg ttt tta gaa gat ege 528 Tyr Asp lie Asn Asn 165 Thr His Arg Phe Thr 170 Leu Phe Leu Glu Asp Arg 175 cgt gaa aag aag ett acc gaa gaa aaa aca tta ggg ett agt gat gca 576 Arg Glu Lys Lys 180 Leu Thr Glu Glu Lys 185 Thr Leu Gly Leu Ser 190 Asp Ala gtg cgt ttt get aat gat caa acc cct tat etc cgt tat ggt att gaa 624 Val Arg Phe 195 Ala Asn Asp Gin Thr 200 Pro Tyr Leu Arg Tyr Gly 205 lie Glu tat ega tat aac ggc ttg tet tgg ttg gaa aeg gta aag ett ttt ttg 672 Tyr Arg 210 Tyr Asn Gly Leu Ser 215 Trp Leu Glu Thr Val 220 Lys Leu Phe Leu gca aag cag aaa ate gaa caa cgt tet get etc caa gag ttt gat att 720 Ala 225 Lys Gin Lys He Glu 230 Gin Arg Ser Ala Leu 235 Gin Glu Phe Asp lie 240 aat aat agg aat aaa ttg gat teg act atg teg ttt gta tat tta caa 768 Asn Asn Arg Asn Lys 245 Leu Asp Ser Thr Met 250 Ser Phe Val Tyr Leu 255 Gin aga cag aat ata get egg gga gaa ttt tea aeg agt cct tta tat tgg 816 Arg Gin Asn lie 260 Ala Arg Gly Glu Phe 265 Ser Thr Ser Pro Leu 270 Tyr Trp ggg ccg agt ege cat cgt tta tet geg aaa ttc gaa ttt cgt gat aag 864 Gly Pro Ser 275 Arg His Arg Leu Ser 280 Ala Lys Phe Glu Phe 285 Arg Asp Lys ttt tta gaa aat atg aat aag cat ttt aeg ttt egg ccg tgg caa ate 912 Phe Leu 290 Glu Asn Met Asn Lys 295 His Phe Thr Phe Arg 300 Pro Trp Gin lie aat aga ttc aga caa caa ggt ega aat aac tat aca gaa gtg ttt ccc 960 Asn 305 Arg Phe Arg Gin Gin Gly 310 Arg Asn Asn Tyr 315 Thr Glu Val Phe Pro 320 gtt aaa tcc ega gag ttt cct ttt tet ett atg gac gac att aag att 1008 Val Lys Ser Arg Glu 325 Phe Ser Phe Ser Leu 330 Met Asp Asp lie Lys 335 lie 136852·序列表.doc 194- 1334884 ggc gaa ttg eta cat etc gga ttg ggc ggt egg tgg gat cac tat aac 1056

Gly Glu Leu Leu His Leu Gly Leu Gly Gly Arg Trp Asp His Tyr Asn 340 345 350 tat aag cca tta tta aat tet cag cat aat ate aac agg aca cag aga 1104

Tyr Lys Pro Leu Leu Asn Ser Gin His Asn lie Asn Arg Thr Gin Arg 355 360 365 tta cct tat cca aaa aca tea tee aaa ttt teg tat caa ttg agt tta 1152

Leu Pro Tyr Pro Lys Thr Ser Ser Lys Phe Ser Tyr Gin Leu Ser Leu 370 375 380 gag tat caa tta cat cca tea cat caa att gca tac cgt tta agt acc 1200

Glu Tyr Gin Leu His Pro Ser His Gin lie Ala Tyr Arg Leu Ser Thr 385 390 395 400 ggt ttt agg gtt ccc cgt gtt gaa gat ett tat ttt gaa gac ega gga 1248

Gly Phe Arg Val Pro Arg Val Glu Asp Leu Tyr Phe Glu Asp Arg Gly 405 410 415 aaa agt tet tea caa ttt ett cct aac ccc gat eta caa ccg gaa act 1296

Lys Ser Ser Ser Gin Phe Leu Pro Asn Pro Asp Leu Gin Pro Glu Thr 420 425 430 gca ctg aat cat gaa ata agt tac cgt ttc caa aat caa tat gee cat 1344

Ala Leu Asn His Glu lie Ser Tyr Arg Phe Gin Asn Gin Tyr Ala His 435 440 445 ttc age gtc ggg ett ttc cgt aca cgt tat cat aac ttt att caa gaa 1392

Phe Ser Val Gly Leu Phe Arg Thr Arg Tyr His Asn Phe lie Gin Glu 450 455 460 cgt gag atg acc tgt gat aaa att cca tat gag tat aat agg act tat 1440

Arg Glu Met Thr Cys Asp Lys lie Pro Tyr Glu Tyr Asn Arg Thr Tyr 465 470 475 480 gga tat tgc aeg cat aat act tat gta atg ttt gtt aat gaa cct gaa I486

Gly Tyr Cys Thr His Asn Thr Tyr Val Met Phe Val Asn Glu Pro Glu 485 490 495 gcc gtg att aaa ggg gtt gaa gta age ggt get tta aat ggg teg gca 1536

Ala Val lie Lys Gly Val Glu Val Ser Gly Ala Leu Asn Gly Ser Ala 500 505 510

ttc gga ett tee gac ggt tta act ttc cgt etc aaa ggg age tac age 1584

Phe Gly Leu Ser Asp Gly Leu Thr Phe Arg Leu Lys Gly Ser Tyr Ser 515 520 525 aaa ggt caa aat cat gac ggc gat ccg tta aaa tet att caa cca tgg 1632

Lys Gly Gin Asn His Asp Gly Asp Pro Leu Lys Ser lie Gin Pro Trp 530 535 540 aca gtg gta acc ggt att gat tac gaa act gaa ggg tgg age gtg agt 1680

Thr Val Val Thr Gly lie Asp Tyr Glu Thr Glu Gly Trp Ser Val Ser 545 550 555 560 ccg age ggg cgt tat agt geg get aaa aaa gcc aaa gat geg ata gaa 1728

Leu Ser Gly Arg Tyr Ser Ala Ala Lys Lys Ala Lys Asp Ala lie Glu 565 570 575 aeg gaa tac aca cat gat aaa aag gtt gtc aaa caa tgg ccg cat tta 1776

Thr Glu Tyr Thr His Asp Lys Lys Val Val Lys Gin Trp Pro His Leu 580 585 590 -195- 136852-序列表.doc is] 1334884 agt cca tcc tac ttt gtt gtt gat ttt acg ggg caa gtt aac etc agt Ser Pro Ser Tyr Phe Val Val Asp Phe Thr Gly Gin Val Asn Leu Ser 595 600 605 aaa aat gtre att ttg aat atg ggg gta ttt aac ttg ttc aat cgt gat Lys Asn Val lie Leu Asn Met Gly Val Phe Asn Leu Phe Asn Arg Asp 610 615 620 tat atg acg tgg gac agt gca tat aac ttg ttt act agg ggg tat act Tyr Met Thr Trp Asp Ser Ala Tyr Asn Leu Phe Thr Arg Gly Tyr Thr 630 635 640 tcc cgt tet gtc cgt get aac age cca ggc att aat egg ttt acc gca Ser Arg Ser Val Arg Ala Asn Ser Pro Gly lie Asn Arg Phe Thr Ala 645 650 655 cca aaa cgt aat ttt get gcc teg gtg gaa att cgt ttt ta Pro Lys Arg Asn Phe Ala Ala Ser Val Glu lie Arg Phe 660 665 1824 1872 1920 1968 2009

<210> 105 <211> 669 <212>龙白質 <213 >敗丨生巴斯i急桿菌 <400> 105

He Arg Gly Val Asp Lys Asp Arg Val Ala Val lie Val Asp Gly lie 1 5 10 is

Pro Gin Ala Glu Ser Thr He Ser Thr Ser Ala Arg Tyr Ser Thr Glu 20 25 30

Arg His Asn Gly Asn lie Asn Asn lie Glu Tyr Glu Asn Val Ser Ser 35 40 45

Leu Lys Val Gin Lys Gly Ala Ala Ser Val Met Tyr Gly Ser Gly Ala 50 55 60

Leu Gly Gly Thr Val Glu Phe Thr Thr Lys Asp He Glu Asp Phe Val 65 70 75 80

Glu Pro Gly Arg His Leu Gly Phe Leu Ser Lys Thr Gly Tyr Thr Ser 85 90 95

Lys Asn Arg Glu Tyr Arg Gin Val lie Gly Val Gly Gly Lys Gly Glu 100 105 110

His Phe Phe Gly Phe Val Gin Leu Thr Lys Arg Trp Gly His Glu Thr 115 120 125 lie Asn Asn Gly Lys Gly Thr Asp lie Leu Gly Glu His Arg Gly Lys 130 135 140

Pro Asn Pro Leu Asn Tyr Tyr Thr Thr Ser Trp Leu Thr Lys Val Gly 145 150 155 160

Tyr Asp lie Asn Asn Thr His Arg Phe Thr Leu Phe Leu Glu Asp Arg 165 170 175

Arg Glu Lys Lys Leu Thr Glu Glu Lys Thr Leu Gly Leu Ser Asp Ala 180 185 190 •196- 136852-序列表.doc

l SI 1334884

Val Arg Phe Ala Asn Asp Gin Thr Pro Tyr Leu Arg Tyr Gly lie Glu 195 200 205

Tyr Arg Tyr Asn Gly Leu Ser Trp Leu Glu Thr Val Lys Leu Phe Leu 210 215 220

Ala Lys Gin Lys lie Glu Gin Arg Ser Ala Leu Gin Glu Phe Asp lie 225 230 235 240

Asn Asn Arg Asn Lys Leu Asp Ser Thr Met Ser Phe Val Tyr Leu Gin 245 250 255

Arg Gin Asn lie Ala Arg Gly Glu Phe Ser Thr Ser Pro Leu Tyr Trp 260 265 270

Gly Pro Ser Arg His Arg Leu Ser Ala Lys Phe Glu Phe Arg Asp Lys 275 280 285

Phe Leu Glu Asn Met Asn Lys His Phe Thr Phe Arg Pro Trp Gin lie 290 295 300

Asn Arg Phe Arg Gin Gin Gly Arg Asn Asn Tyr Thr Glu Val Phe Pro 305 310 315 320

Val Lys Ser Arg Glu Phe Ser Phe Ser Leu Met Asp Asp lie Lys lie 325 330 335

Gly Glu Leu Leu His Leu Gly Leu Gly Gly Arg Trp Asp His Tyr Asn 340 345 350

Tyr Lys Pro Leu Leu Asn Ser Gin His Asn lie Asn Arg Thr Gin Arg 355 360 365

Leu Pro Tyr Pro Lys Thr Ser Ser Lys Phe Ser Tyr Gin Leu Ser Leu 370 375 380

Glu Tyr Gin Leu His Pro Ser His Gin lie Ala lyr Arg Leu Ser Thr 385 390 395 400

Gly Phe Arg Val Pro Arg Val Glu Asp Leu Tyr Phe Glu Asp Arg Gly 405 410 415

Lys Ser Ser Ser Gin Phe Leu Pro Asn Pro Asp Leu Gin Pro Glu Thr 420 425 430

Ala Leu Asn His Glu lie Ser Tyr Arg Phe Gin Asn Gin Tyr Ala His 435 440 445

Phe Ser Val Gly Leu Phe Arg Thr Arg Tyr His Asn Phe lie Gin Glu 450 455 460

Arg Glu Met Thr Cys Asp Lys lie Pro Tyr Glu Tyr Asn Arg Thr Tyr 465 470 475 480

Gly Tyr Cys Thr His Asn Thr Tyr Val Met Phe Val Asn Glu Pro Glu 485 490 495

Ala Val lie Lys Gly Val Glu Val Ser Gly Ala Leu Asn Gly Ser Ala 500 505 510

Phe Gly Leu Ser Asp Gly Leu Thr Phe Arg Leu Lys Gly Ser Tyr Ser 515 520 525 •197· 136852-序列表.doc 1S1 1334884

Lys Gly Gin Asn His Asp Gly Asp Pro Leu Lys Ser lie Gin Pro Trp 530 535 540

Thr Val Val Thr Gly lie Asp Tyr Glu Thr Glu Gly Trp Ser Val Ser 545 550 555 560

Leu Ser Gly Arg Tyr Ser Ala Ala Lys Lys Ala Lys Asp Ala lie Glu 565 570 575

Thr Glu Tyr Thr His Asp Lys Lys Val Val Lys Gin Trp Pro His Leu 580 585 590

Ser Pro Ser Tyr Phe Val Val Asp Phe Thr Gly Gin Val Asn Leu Ser 595 600 605

Lys Asn Val lie Leu Asn Met Gly Val Phe Asn Leu Phe Asn Arg Asp 610 615 620

Tyr Met Thr Trp Asp Ser Ala Tyr Asn Leu Phe Thr Arg Gly Tyr Thr 625 630 635 640

Ser Arg Ser Val Arg Ala Asn Ser Pro Gly lie Asn Arg Phe Thr Ala

645 650 65S

Pro Lys Arg Asn Phe Ala Ala Ser Val Glu lie Arg Phe 660 665

<210> 106 <211> 908 <212> DNA <213>改叔，:*1巴斯德桴茁 <220>

<223> lgtC <220> <221> CDS <222> (1) . . (906) <400> 106 atg Met 1 aat Asn att lie tta Leu ttt Phe 5 9tt Val tet Ser gat Asp gat Asp gtt Val 10 tat Tyr get Ala aaa Lys cat His ctg Leu 15 gtg Val 48 gtt Val gcg Ala att lie aaa Lys 20 age Ser att lie ata lie aat Asn cat His 25 aat Asn gaa Glu aaa Lys ggt Gly att lie 30 tea Ser ttt Phe 96 tat att ttt gat ttg ggt ata aag gat gaa aat aag aga aat ate aat 144 Tyr lie Phe 35 Asp Leu Gly lie Lys 40 Asp Glu Asn Lys Arg 45 Asn lie Asn gat att gtt tet tet tat gga agt gaa gtc aac ttt att get gtg aat 192 Asp lie 50 Val Ser Ser Tyr Gly 5S Ser Glu Val Asn Phe 60 lie Ala Val Asn gag aaa gaa ttt gag agt ttt cct gtt caa att agt tat att tet tta 240 Glu 65 Lys Glu Phe Glu Ser 70 Phe Pro Val Gin lie 75 Ser Tyr lie Ser Leu 80

-198- 136852·序列表.doc IS1 1334884 gca aca cat gca agg eta aaa geg gca gag tat ttg ccg gat aat tta 288

Ala Thr Tyr Ala Arg Leu Lys Ala Ala Glu Tyr Leu Pro Asp Asn hen 85 90 95 aat aaa att att tat tta gat gtt gat gtt ttg gtt ttt aac tea tta 336

Asn Lys He He Tyr Leu Asp val Asp Val Leu Val Phe Asn Ser Leu 100 105 110 gaa atg tta tgg aat gtt gat gtt aat aat ttt ett acc gca gcc tgt 384

Glu Met Leu Trp Asn Val Asp Val Asn Asn Phe Leu Thr Ala Ala Cys 115 120 125 tat gat tet ttc ate gaa aat gaa aag tet gag cat aaa aaa teg att 432

Tyr Asp Ser Phe lie Glu Asn Glu Lys Ser Glu His Lys Lys Ser lie 130 135 140 tea atg tea gat aag gaa tat tat ttt aat gca gga gta atg eta ttt 480

Ser Met Ser Asp Lys Glu Tyr Tyr Phe Asn Ala Gly Val Met Leu Phe 145 150 155 160 aat tta gat gaa tgg egg aag atg gat gta ttc tea aga get tta gac S28

Asn Leu Asp Glu Trp Arg Lys Met Asp Val Phe Ser Arg Ala Leu Asp 165 170 175 ctg tta get atg tat cct aat caa atg att tat cag gat caa gat ata 576

Leu Leu Ala Met Tyr Pro Asn Gin Met lie Tyr Gin Asp Gin Asp lie 1B0 185 190 ttg aat ate ett ttt agg aat aaa gtc tgt taC tta gat tgc aga ttt 624

Leu Asn lie Leu Phe Arg Asn Lys Val Cys Tyr Leu Asp Cys Arg Phe 195 200 205 aat ttc atg cca aat caa ett gaa aga ata aaa caa tac cat aaa gga 672

Asn Phe Met Pro Asn Gin Leu Glu Arg lie Lys Gin Tyr His Lys Gly 210 215 220 aaa ttg age aac tta cat tet tta gaa aaa aca aeg atg cct gtc gtt 720

Lys Leu Ser Asn Leu His Ser Leu Glu Lys Thr Thr Met Pro Val Val 225 230 235 240 att tea cat tat tgt ggt cca gaa aaa geg tgg cat geg gat tgt aaa 768 lie Ser His Tyr Cys Gly Pro Glu Lys Ala Trp His Ala Asp Cys Lys 245 250 255

cat ttt aat gta tat ttc tat cag aaa ata tta gca gaa ata aeg aga 816

His Phe Asn Val Tyr Phe Tyr Gin Lys lie Leu Ala Glu lie Thr Arg 260 265 270 ggc aeg gat aaa gaa ege gta tta tet ata aaa act tat etc aag gcc 864

Gly Thr Asp Lys Glu Arg Val Leu Ser lie Lys Thr Tyr Leu Lys Ala 275 260 285 ttg att aga agg att aga tat aaa ttc aaa tat caa gtc tat ta 908

Leu lie Arg Arg He Arg Tyr Lys Phe Lys Tyr Gin Val Tyr 290 295 300 <210> 107 <211> 302 <212>蛋白贫 <2α3> «Wtt.it巴斯德桴璃 <400> 107 136852-序列表.doc ，丨 99- 1 S1 1334884

Met Asn lie Leu Phe Val Ser Asp Asp Val Tyr Ala Lys His Leu Val 15 10 15

Val Ala lie Lys Ser lie lie Asn His Asn Glu Lys Gly lie Ser Phe 20 25 30

Tyr lie Phe Asp Leu Gly lie Lys Asp Glu Asn Lys Arg Asn lie Asn 35 40 45

Asp lie Val Ser Ser Tyr Gly Ser Glu Val Asn Phe lie Ala Val Asn 50 55 60

Glu Lys Glu Phe Glu Ser Phe Pro Val Gin lie Ser Tyr lie Ser Leu 65 70 75 80

Ala Thr Tyr Ala Arg Leu Lys Ala Ala Glu Tyr Leu Pro Asp Asn Leu 85 90 95

Asn Lys lie lie Tyr Leu Asp Val Asp Val Leu Val Phe Asn Ser Leu 100 105 110

Glu Met Leu Trp Asn Val Asp Val Asn Asn Phe Leu Thr Ala Ala Cys 115 120 125

Tyr Asp Ser Phe lie Glu Asn Glu Lys Ser Glu His Lys Lys Ser lie 130 135 140

Ser Met Ser Asp Lys Glu Tyr Tyr Phe Asn Ala Gly Val Met Leu Phe 145 150 X55 160

Asn Leu Asp Glu Trp Arg Lys Met Asp Val Phe Ser Arg Ala Leu Asp 165 170 175

Leu Leu Ala Met Tyr Pro Asn Gin Met lie Tyr Gin Asp Gin Asp lie 180 185 190

Leu Asn lie Leu Phe Arg Asn Lys Val Cys Tyr Leu Asp Cys Arg Phe 195 200 205

Asn Phe Met Pro Asn Gin Leu Glu Arg lie Lys Gin Tyr His Lys Gly 210 215 220

Lys Leu Ser Asn Leu His Ser Leu Glu Lys Thr Thr Met Pro Val Val

225 230 235 240 lie Ser His Tyr Cys Gly Pro Glu Lys Ala Trp His Ala Asp Cys Lys 245 250 255

His Phe Asn Val Tyr Phe Tyr Gin Lys lie Leu Ala Glu lie Thr Arg 260 265 270

Gly Thr Asp Lys Glu Arg Val Leu Ser He Lys Thr Tyr Leu Lys Ala 275 280 285

Leu lie Arg Arg lie Arg Tyr Lys Phe Lys Tyr Gin Val Tyr 290 295 300 <210> 108 <211> 2054

<212> DNA

<213> 巴埘达WM 200 - 136852·序列表 _doc IVl 1334884 <220> <223> pnp <220> <221> CDS <222> ⑴..（2052)

<400> 108 atg gca agt atg gat gat act act gtg ttt gtc aca gtg gtt gcc aaa 48 Met 1 Ala Ser Met Asp 5 Asp Thr Thr Val Phe 10 Val Thr Val Val Ala 15 Lys aaa gat gtg aaa gaa ggt: caa gac ttc ttc cca tta act gtt aac tat 96 Lys Asp Val Lys 20 Glu Gly Gin Asp Phe 25 Phe Pro Leu Thr Val 30 Asn Tyr caa gag cgt act tat get gca ggc cgt att cct ggt ggc ttt ttc aaa 144 Gin Glu Arg 35 Thr Tyr Ala Ala Gly Arg 40 lie Pro Gly Gly 4S Phe Phe Lys cgt gaa ggt cgt cct tet gaa ggc gaa act tta att get cgt tta att 192 Arg Glu Gly Arg 50 Pro Ser Glu 55 Gly Glu Thr Leu lie 60 Ala Arg Leu lie gac cgt cca att cgt cct ett ttc cca gaa ggt ttt tat aac gaa ate 240 Asp 65 Arg Pro lie Arg Pro 70 Leu Phe Pro Glu Gly Phe 75 Tyr Asn Glu lie 80 caa ate gtg geg aca gtg gtg tet gtt aat ccg caa att tgt cca gat 288 Gin lie Val Ala Thr 85 Val Val Ser Val Asn 90 Pro Gin lie Cys Pro Asp 95 tta gtg gca atg ate ggt gca tet geg gca ett tet tta tea ggt gtg 336 Leu Val Ala Met 100 lie Gly Ala Ser Ala 105 Ala Leu Ser Leu Ser 110 Gly Val cca ttt aat ggc cct ate ggt geg gca cgt gtt ggt ttt att gat gat 384 Pro Phe Asn Gly 115 Pro lie Gly Ala 120 Ala Arg Val Gly Phe 125 lie Asp Asp caa ttt gtg tta aac cca acc atg aac gag caa aaa caa age cgt tta 432 Gin Phe 130 Val Leu Asn Pro Thr 135 Met Asn Glu Gin Lys 140 Gin Ser Arg Leu gac ttg gtt gtc geg gga aca gat aaa geg gtg tta atg gtg gaa tet 480 Asp 145 Leu Val val Ala Gly Thr 150 Asp Lys Ala Val 155 Leu Met Val Glu Ser 160 gaa gcc gat gta tta acc gaa gaa caa atg tta get geg gtg gtg ttt 528 Glu Ala Asp Val Leu 165 Thr Glu Glu Gin Met 170 Leu Ala Ala Val Val 175 Phe ggt cat cag caa caa caa gtg gtg att gac geg ate aaa gaa ttt acc 576 Gly His Gin Gin 180 Gin Gin Val Val lie 185 Asp Ala lie Lys Glu 190 Phe Thr gca gaa gcc ggt aaa ccg cgt tgg gat tgg gtg gca cct gaa cca aat 624 Ala Glu Ala 195 Gly Lys Pro Arg Trp 200 Asp Trp Val Ala Pro 205 Glu Pro Asn acc geg tta ate gaa aaa gtg aaa geg att gca gaa geg cgt tta ggc 672 Thr Ala 210 Leu lie Glu Lys Val 215 Lys Ala lie Ala Glu 220 Ala Arg Leu Gly 136852·序列表.doc -201 - 1334884

gaa gca tac cgt ate act gaa aaa caa gca cgt tat gaa caa att gat 720 Glu 225 Ala Tyr Arg lie Thr 230 Glu Lys Gin Ala Arg 235 Tyr Glu Gin lie Asp 240 gcg att aaa get gat gtg att gca caa ate aca get gaa gta gca gaa 768 Ala lie Lys Ala Asp 245 Val lie Ala Gin lie 250 Thr Ala Glu Val Ala 255 Glu ggc gaa gac ate agt gaa ggg aaa att gtc gat att ttc acc gca ett 816 Gly Glu Asp lie 260 Ser Glu Glv Lys lie 265 Val Asp lie Phe Thr 270 Ala Leu gaa age caa ate gta cgt age cgt ate att get ggt gaa cca cgt att 864 Glu Ser Gin 275 He Val Arg Ser Arg 280 lie lie Ala Gly Glu 285 Pro Arg lie gat ggt cgt aca gtg gat act gtt cgt gca tta gat att tgt act ggt 912 Asp Gly 290 Arg Thr Val Asp Thr 295 Val Arg Ala Leu Asp 300 lie Cys Thr Gly gtt tta cca cgt aca cac ggt tct gcg att ttc acc cgt ggt gaa aca 960 Val 305 Leu Pro Arg Thr His 310 Gly Ser Ala lie Phe 315 Thr Arg Gly Glu Thr 320 cag gcg tta get gtc gcg aca tta ggt aca gaa cgt gat gca caa att 1008 Gin Ala Leu Ala Val 325 Ala Thr Leu Gly Thr 330 Glu Arg Asp Ala Gin 335 lie att gat gaa tta aca ggt gag cgt tea gat cac ttc tta ttc cac tac 1056 lie Asp Glu Leu 340 Thr Gly Glu Arg Ser 345 Asp His Phe Leu Phe 350 His Tyr aac ttc ccg cca tat tct gtg ggt gaa acc ggt atg att ggt tea cca 1104 Asn Phe Pro 355 Pro Tyr Ser Val Gly Glu 360 Thr Gly Met He Gly 365 Ser Pro aaa cgt cgt gaa att ggt cat ggt cgt tta gcg aaa ege ggt gta get 1152 Lys Arg 370 Arg Glu lie Gly His 375 Gly Arg Leu Ala Lys 380 Arg Gly Val Ala gca gtg atg cca aca ett gcc gag ttc ccg tat gtg gta cgt gtt gtc 1200 Ala 385 Val Met Pro Thr Leu 390 Ala Glu Phe Pro Tyr 395 Val Val Arg Val Val 400 tct gaa ate aca gaa tea aat ggt tct tct tct atg gca teg gtt tgt 1248 Ser Glu lie Thr Glu 405 Ser Asn Gly Ser Ser 410 Ser Met Ala Ser Val 415 Cys ggt gcg tct tta gca tta atg gat gcg ggt gta cca att aaa gcg gcg 1296 Gly Ala Ser Leu 420 Ala Leu Met Asp Ala 425 Gly Val Pro lie Lys 430 Ala Ala gtt gca ggt att gca atg ggc CCa gtc aaa gaa gac gaa aaa ttt gtg 1344 Val Ala Gly 435 lie Ala Met Gly Leu 440 Val Lys Glu Asp Glu 445 Lys Phe Val gtg ett tea gac ate tta ggt gat gaa gat cac tta ggt gac atg gac 1392 Val Leu 450 Ser Asp lie Leu Gly 455 Asp Glu Asp His Leu 460 Gly Asp Met Asp ttc aaa gtc gcg ggt aca ege aeg ggt gtg aeg gca tta caa atg gat 1440 Phe 465 Lys Val Ala Gly Thr 470 Arg Thr Gly Val Thr 475 Ala Leu Gin Met Asp 480 136852-序列表.doc •202·

1 SI 1488 ate aaa ate gaa ggt ate aca gca gaa ate atg caa att geg tta aac lie Lys lie Glu Gly lie Thr Ala Glu lie Met Gin lie Ala Leu Asn 485 490 495 caa geg aaa age gca cgt tta cac att tta ggt gtg atg gag caa geg 1536

Gin Ala Lys Ser Ala Arg Leu His lie Leu Gly Val Met Glu Gin Ala 500 505 510 ate cca geg cca cgt geg gat att tet gat ttt gca ccg cgt att tac 1584 lie Pro Ala Pro Arg Ala Asp lie Ser Asp Phe Ala Pro Arg lie Tyr 515 520 525 act atg aaa att gat ccg aag aaa ate aaa gat gtg ate ggt aaa ggt 1632

Thr Met Lys lie Asp Pro Lys Lys He Lys Asp Val lie Gly Lys Gly 530 535 540 ggt gca acc att cgt gee tta aca gaa gaa aca ggt acc tea att gat 1680

Gly Ala Thr lie Arg Ala Leu Thr Glu Glu Thr Gly Thr Ser lie Asp 545 550 555 560 ate gat gat gat ggt aeg gtg aag att get geg gtt gat ggc aat tea 1728

He Asp Asp Asp Gly Thr Val Lys He Ala Ala Val Asp Gly Asn Ser 565 570 575 gca aaa gag gtg atg geg cgt att gaa gat att act gca gaa gtt gaa 1776

Ala Lys Glu Val Met Ala Arg He Glu Asp lie Thr Ala Glu Val Glu 580 585 590 geg ggt gca gtg tat aaa ggt aaa gtt act cgt tta get gat ttt ggt 1824

Ala Gly Ala Val Tyr Lys Gly Lys Val Thr Arg beu Ala Asp Phe Gly 595 600 605 gee ttc gtt tet ate gta ggt aac aaa gaa ggc tta gtg cat att tet 1872

Ala Phe Val Ser lie Val Gly Asn Lys Glu Gly Leu Val His lie Ser 610 615 620 caa ate geg gaa gag cgt gtt gag aaa gtg agt gat tat ct:t gca gtg 1920

Gin lie Ala Glu Glu Arg Val Glu Lys Val Ser Asp Tyr Leu Ala Val 625 630 635 640 ggg caa gaa gtg act gtt aaa gtg gtt gag att gat cgt caa ggt cgt 1968

Gly Gin Glu Val Thr Val Lys Val Val Glu He Asp Arg Gin Gly Arg 645 650 655 att cgt tta acc atg aaa gaa gtt gca cca aag caa gaa cac gtt gat 2016 lie Arg Leu Thr Met Lys Glu Val Ala Pro Lys Gin Glu His Val Asp 660 665 670 tet gtt gtc gca gac gtt gee gca gaa gaa aac gca ta 2054

Ser Val Val Ala Asp Val Ala Ala Glu Glu Asn Ala 675 680 <210> 109 <211> 684 <212> 畓白ΐ? <213> 收血代巴斯德·样逋 <400> 109

Met Ala Ser Met Asp Asp Thr Thr Val Phe Val Thr Val Val Ala Lys 15 10 15

Lys Asp Val Lys Glu Gly Gin Asp Phe Phe Pro Leu Thr Val Asn Tyr •203· 136852·序列表.doc i S] 20 25 30

Gin Glu Arg Thr Tyr Ala Ala Gly Arg lie Pro Gly Gly Phe Phe Lys 35 40 45

Arg Glu Gly Arg Pro Ser Glu Gly Glu Thr Leu lie Ala Arg Leu lie SO 55 60

Asp Arg Pro lie Arg Pro Leu Phe Pro Glu Gly Phe Tyr Asn Glu He 65 70 75 80

Gin lie Val Ala Thr Val Val Ser Val Asn Pro Gin lie Cys Pro Asp 85 90 95

Leu Val Ala Met lie Gly Ala Ser Ala Ala Leu Ser Leu Ser Gly Val 100 105 110

Pro Phe Asn Gly Pro He Gly Ala Ala Arg Val Gly Phe lie Asp Asp 115 120 125

Gin Phe Val Leu Asn Pro Thr Met Asn Glu Gin Lys Gin Ser Arg Leu 130 135 140

Asp Leu Val Val Ala Gly Thr Asp Lys Ala Val Leu Met Val Glu Ser 145 150 155 160

Glu Ala Asp Val Leu Thr Glu Glu Gin Met Leu Ala Ala Val Val Phe 165 170 175

Gly His Gin Gin Gin Gin Val Val lie Asp Ala lie Lys Glu Phe Thr 180 185 190

Ala Glu Ala Gly Lys Pro Arg Trp Asp Trp Val Ala Pro Glu Pro Asn 195 200 205

Thr Ala Leu lie Glu Lys Val Lys Ala lie Ala Glu Ala Arg Leu Gly 210 215 220

Glu Ala Tyr Arg lie Thr Glu Lys Gin Ala Arg Tyr Glu Gin lie Asp 225 230 235 240

Ala He Lys Ala Asp Val lie Ala Gin lie Thr Ala Glu Val Ala Glu 245 250 255

Gly Glu Asp lie Ser Glu Gly Lys lie Val Asp lie Phe Thr Ala Leu 260 265 270

Glu Ser Gin lie Val Arg Ser Arg lie lie Ala Gly Glu Pro Arg lie 275 280 285

Asp Gly Arg Thr Val Asp Thr Val Arg Ala Leu Asp He Cys Thr Gly 290 295 300

Val Leu Pro Arg Thr His Gly Ser Ala lie Phe Thr Arg Gly Glu Thr 305 310 315 320

Gin Ala Leu Ala Val Ala Thr Leu Gly Thr Glu Arg Asp Ala Gin lie 325 330 335 lie Asp Glu Leu Thr Gly Glu Arg Ser Asp His Phe Leu Phe His Tyr 340 345 350

Asn Phe Pro Pro Tyr Ser Val Gly Glu Thr Gly Met lie Gly Ser Pro •204- 136852·序列表.doc 1334884

355 360 365

Lys Arg Arg Glu lie Gly His Gly Arg Leu Ala Lys Ara Gly Val Ala 370 375 380

Ala Val Met Pro Thr Leu Ala Glu Phe Pro Tyr Val Val Arg Val Val 385 390 395 400

Ser Glu lie Thr Glu Ser Asn Gly Ser Ser Ser Met Ala Ser Val Cys 405 410 415

Gly Ala Ser Leu Ala Leu Met Asp Ala Gly Val Pro He Lys Ala Ala 420 425 430

Val Ala Gly He Ala Met Gly Leu Val Lys Glu Asp Glu Lys Phe Val 435 440 445

Val Leu Ser Asp lie Leu Gly Asp Glu Asp His Leu Gly Asp Met Asp 450 455 460

Phe Lys Val Ala Gly Thr Arg Thr Gly Val Thr Ala Leu Gin Met Asp 465 470 475 480 lie Lys lie Glu Gly lie Thr Ala Glu lie Met Gin lie Ala Leu Asn 4Θ5 490 495

Gin Ala Lys Ser Ala Arg Leu His lie Leu Gly Val Met Glu Gin Ala 500 505 510 lie Pro Ala Pro Arg Ala Asp lie Ser Asp Phe Ala Pro Arg lie Tyr 515 520 525

Thr Met Lys lie Asp Pro Lys Lys lie Lys Asp Val lie Gly Lys Gly 530 535 540

Gly Ala Thr lie Arg Ala Leu Thr Glu Glu Thr Gly Thr Ser lie Asp 545 550 555 560 lie Asp Asp Asp Gly Thr Val Lys lie Ala Ala Val Asp Gly Asn Ser 565 570 575

Ala Lys Glu Val Met Ala Arg lie Glu Asp lie Thr Ala Glu Val Glu 580 585 590

Ala Gly Ala Val Tyr Lys Gly Lys Val Thr Arg Leu Ala Asp Phe Gly 595 600 605

Ala Phe Val Ser lie Val Gly Asn Lys Glu Gly Leu Val His lie Ser 610 615 620

Gin lie Ala Glu Glu Arg Val Glu Lys Val Ser Asp Tyr Leu Ala Val 625 630 635 640

Gly Gin Glu Val Thr Val Lys Val Val Glu lie Asp Arg Gin Gly Arg 645 650 655 lie Arg Leu Thr Met Lys Glu Val Ala Pro Lys Gin Glu His Val Asp 660 665 670

Ser Val Val Ala Asp Val Ala Ala Glu Glu Asn Ala 675 680 136852·序列表.doc -205-

I SI 1334884

<210> 110 <2X1> 1514 <212> DNA 欧血·丨生巴斯谂样ύίί <220>

<223> purF <220> <221> CDS <222> (1) . . (1512) <400> 110 atg tgt ggt att gtt ggt att gtt age caa age ccc gtt aac caa tea 48

Met Cys Gly He Val Gly lie Val Ser Gin Ser Pro Val Asn Gin Ser 15 10 15 att tat gat geg tta acc tta ttg caa cac ege ggg caa gac gee gee 96

He Tyr Asp Ala Leu Thr Leu Leu Gin His Arg Gly Gin Asp Ala Ala 20 25 30

ggg att gta acc gta gat gat gaa aac ega ttc ege ttg cgt aaa geg 144

Gly He Val Thr Val Asp Asp Glu Asn Arg Phe Arg Leu Arg Lys Ala 35 40 45 aac ggg tta gtc age gat gta ttt gaa caa gtt cat atg tta cgt tta 192

Asn Gly Leu Val Ser Asp Val Phe Glu Gin Val His Met Leu Arg Leu 50 55 60 caa ggc aat get ggc att gga cat gtt cgt tat cct aeg get ggg age 240

Gin Gly Asn Ala Gly He Gly His Val Arg Tyr Pro Thr Ala Gly Ser 65 70 75 80 tea agt gtc tet gaa geg caa cct ttt tat gta aat teg cct tat ggc 26β

Ser Ser Val Ser Glu Ala Gin Pro Phe Tyr Val Asn Ser Pro Tyr Gly 85 90 95 tta acc tta gtg cat aat ggt aac ttg acc aat tea agt gaa tta aaa 336

Leu Thr Leu Val His Asn Gly Asn Leu Thr Asn Ser Ser Glu Leu Lys 100 105 110 gaa aag tta ttt cgt etc gca cgt ege cat gta aat acc aat tea gat 384

Glu Lys Leu Phe Arg Leu Ala Arg Arg His Val Asn Thr Asn Ser Asp 115 120 125

tet gaa tta tta etc aat ate tta gee aat cac ett gat cac ttc gaa 432

Ser Glu Leu Leu Leu Asn lie Leu Ala Asn His Leu Asp His Phe Glu 130 135 140 aaa tac caa tta gat ccg caa gat gta ttc agt get gtc aaa caa aeg 480

Lys Tyr Gin Leu Asp Pro Gin Asp Val Phe Ser Ala Val Lys Gin Thr 145 150 155 160 cat cag gat att cgt ggt get tat get tgt ate gee aeg att att ggt 528

His Gin Asp lie Arg Gly Ala Tyr Ala Cys lie Ala Met lie lie Gly 165 170 175 cat ggt atg gtc geg ttt cgt gat ccg aac ggt ate cgt ccg tta gtg 576

His Gly Met Val Ala Phe Arg Asp Pro Asn Gly lie Arg Pro Leu Val 180 185 190 eta ggg aaa ege gag gaa aat ggc aaa aca gag tat aeg ttt gee tee 624

Leu Gly Lys Arg Glu Glu Asn Gly Lys Thr Glu Tyr Met Phe Ala Ser -206· q6852-序列表.doc

l SI 6721334884

195 200 205 gaa agt ate gca tta gat aca gtg ggt ttt gag ttt gta ega gat gta

Glu Ser He Ala Leu Asp Thr Val Gly Phe Glu Phe Val Arg Asp Val 210 215 220 caa ccc ggc gaa geg att tat gtc aeg ttt gaa ggg gaa atg tat get

Gin Pro Gly Glu Ala lie Tyr Val Thr Phe Glu Gly Glu Met Tyr Ala 225 230 235 240 cag caa tgc gca gac aaa cca aca tea aca cct tgt att ttt gaa tac

Gin Gin Cys Ala Asp Lys Pro Thr Leu Thr Pro Cys lie Phe Glu Tyr 245 250 255 gtc tat ttt gca cgt cca gac tet tgc ate gat ggg gtt tet gtt tat

Val Tyr Phe Ala Arg Pro Asp Ser Cys lie Asp Gly Val Ser Val Tyr 260 265 270 get gee cgt gtt cat atg gga caa cgt tta ggt gaa aaa att gca egg

Ala Ala Arg Val His Met Gly Gin Arg Leu Gly Glu Lys lie Ala Arg 275 280 285 gaa tgg geg gat gtg gat gat att gat gtg gtc att cct gtg cct gaa

Glu Trp Ala Asp Val Asp Asp lie Asp Val Val lie Pro Val Pro Glu 290 295 300 acc tet aac gat att get tta cgt att geg ege gtg tta aat aaa ccg

Thr Ser Asn Asp lie Ala Leu Arg lie Ala Arg Val Leu Asn Lys Pro 305 310 315 320 tat cgt caa ggt ttt gtg aaa aat ege tat gta gga cgt aeg ttt att

Tyr Arg Gin Gly Phe Val Lys Asn Arg Tyr Val Gly Arg Thr Phe lie 325 330 335 atg ccg ggg cag gca ttg ega gtc agt tet gtt aga cgt aaa etc aat

Met Pro Gly Gin Ala Leu Arg Val Ser Ser Val Arg Arg Lys Leu Asn 340 345 350 acc att get tea gaa ttt aaa gat aag aat gtg tta tta gtt gac gac

Thr lie Ala Ser Glu Phe Lys Asp Lys Asn Val Leu Leu Val Asp Asp 355 360 365 teg att gta cgt ggt acc aeg tet gaa caa att gtc gaa atg geg aga

Ser lie Val Arg Gly Thr Thr Ser Glu Gin lie Val Glu Met Ala Arg 370 375 380 geg gca ggt geg aag aaa att tat ttt gee tet get gca cca gaa att

Ala Ala Gly Ala Lys Lys He Tyr Phe Ala Ser Ala Ala Pro Glu lie 385 390 395 400 cgt tat cca aat gtg tat ggt att gat atg cca acc aaa aat gaa ttg

Arg Tyr Pro Asn Val Tyr Gly lie Asp Met Pro Thr Lys Asn Glu Leu 405 410 415 ate get tat ggt cgt gat gta gat gaa att get aac tta att ggt gtg lie Ala Tyr Gly Arg Asp Val Asp Glu lie Ala Asn Leu lie Gly Val 420 425 430 gat aaa teg att etc caa gat ttg gat geg tta act ggt tet gtg caa

Asp Lys Leu lie Phe Gin Asp Leu Asp Ala Leu Thr Gly Ser Val Gin 435 440 445 caa gaa aat cca agt att caa gac tet gat tgt teg gtg ttt aca ggg 136852·序列表.doc -207* 720 768 816 864 912 960 1008 1056 1104 1152 1200 1248 1296 1344 1392 IVi 14401334884

Gin Glu Asn Pro Ser lie Gin Asp Phe Asp Cys Ser Val Phe Thr Gly 450 455 460 gtt tat gtg acg ggc gat att aca cct gaa tat ctg gat aat att gca Val Tyr Val Thr Gly Asp lie Thr Pro Glu Tyr Leu Asp Asn lie Ala 465 470 475 480 gaa cag cgt aat gat ate gcc aag aaa aag cgt gaa aaa gat get acc Glu Gin Arg Asn Asp lie Ala Lys Lys Lys Arg Glu Lys Asp Ala Thr 485 490 495 aat ett gaa atg cac aat gaa aaa ta Asn Leu Glu Met His Asn Glu Lys 500 1488 1514

<210> 111 <211> 504 <212>丧白货 <213>畋血饭巴斯迭桴菌 <400> 111 Met Cys Gly lie Val Gly lie Val Ser Gin Ser Pro Val Asn Gin Ser 15 10 15 He Tyr Asp Ala Leu Thr Leu Leu Gin His Arg Gly Gin Aep Ala Ala 20 25 30 Gly He Val Thr Val Asp Asp Glu Asn Arg Phe Arg Leu Arg Lys Ala 35 40 45 Asn Gly Leu Val Ser Asp Val Phe Glu Gin Val His Met Leu Arg Leu 50 55 60 Gin Gly Asn Ala Gly lie Gly His Val Arg Tyr Pro Thr Ala Gly Ser 65 70 75 Θ0 Ser Ser Val Ser Glu Ala Gin Pro Phe Tyr Val Asn Ser Pro Tyr Gly 85 90 95 Leu Thr Leu Val His Asn Gly Asn Leu Thr Asn Ser Ser Glu Leu Lys 100 105 110 Glu Lys Leu Phe Arg Leu Ala Arg Arg His Val Asn Thr Asn Ser Asp 115 120 125 Ser Glu Leu Leu Leu Asn lie Leu Ala Asn His Leu Asp His Phe Glu 130 135 140 Lys Tyr Gin Leu Asp Pro Gin Asp Val Phe Ser Ala Val Lys Gin Thr 145 150 155 160 His Gin Asp lie Arg Gly Ala Tyr Ala Cys lie Ala Met lie lie Gly .165 170 175 His Gly Met Val Ala Phe Arg Asp Pro Asn Gly lie Arg Pro Leu Val 180 IBS 190 Leu Gly Lys Arg Glu Glu Asn Gly Lys Thr Glu Tyr Met Phe Ala Ser 195 200 205 Glu Ser He Ala Leu Asp Thr Val Gly Phe Glu Phe Val Arg Asp Val 210 215 220 136852-序列表.doc -208- IS] 1334884

Gin Pro Gly Glu Ala lie Tyr Val Thr Phe Glu Gly Glu Met Tyr Ala 225 230 235 240

Gin Gin Cys Ala Asp Lys Pro Thr Leu Thr Pro Cys lie Phe Glu Tyr 245 250 255

Val Tyr Phe Ala Arg Pro Asp Ser Cys lie Asp Gly Val Ser Val Tyr 260 265 270

Ala Ala Arg Val His Met Gly Gin Arg Leu Gly Glu Lys lie Ala Arg 275 280 285

Glu Trp Ala Asp Val Asp Asp lie Asp Val Val lie Pro Val Pro Glu 290 295 300

Thr Ser Asn Asp lie Ala Leu Arg lie Ala Arg Val Leu Asn Lys Pro 305 310 315 320

Tyr Arg Gin Gly Phe Val Lys Asn Arg Tyr Val Gly Arg Thr Phe He 325 330 335

Met Pro Gly Gin Ala Leu Arg Val Ser Ser Val Arg Arg Lys Leu Asn 340 345 350

Thr lie Ala Ser Glu Phe Lys Asp Lys Asn Val Leu Leu Val Asp Asp 355 360 365

Ser lie Val Arg Gly Thr Thr Ser Glu Gin lie Val Glu Met Ala Arg 370 375 380

Ala Ala Gly Ala Lys Lys lie Tyr Phe Ala Ser Ala Ala Pro Glu lie 385 390 395 400

Arg Tyr Pro Asn Val Tyr Gly He Asp Met Pro Thr Lys Asn Glu Leu 405 410 415

He Ala Tyr Gly Arg Asp Val Asp Glu lie Ala Asn Leu lie Gly Val 420 425 430

Asp Lys Leu lie Phe Gin Asp Leu Asp Ala Leu Thr Gly Ser Val Gin 435 440 445

Gin Glu Asn Pro Ser lie Gin Asp Phe Asp Cys Ser Val Phe Thr Gly 450 455 460

Val Tyr Val Thr Gly Asp He Thr Pro Glu Tyr Leu Asp Asn lie Ala 465 470 475 480

Glu Gin Arg Asn Asp lie Ala Lys Lys Lys Arg Glu Lys Asp Ala Thr 485 490 495

Asn Leu Glu Met His Asn Glu Lys 500 <210> 112 <211> 989 <212> DNA <213> 改ώιΗ巴Wit桴适 <220> <223> rci 136852·序列表.doc -209· IS! 1334884 <220> <221> CDS <222> ⑴· ·（987) <400> 112 atg gca aca ata aga aaa cgt ggt aac tea tat cgt get gag ata age 48

Met Ala Thr lie Arg Lys Arg Gly Asn Ser Tyr Arg Ala Glu lie Ser 1 5 10 15 aaa aac gga gta agg aaa tea gca aca ttt aag act aaa tea gaa get 96

Lys Asn Gly Val Arg Lys Ser Ala Thr Phe Lys Thr Lys Ser Glu Ala 20 25 30 aat geg tgg get gtt gac gag gag aga aaa tta get gat ttg gca aaa 144

Asn Ala Trp Ala Val Asp Glu Glu Arg Lys Leu Ala Asp Leu Ala Lys 35 40 4*5 ggt ate get cca gat att att ttt aga gat gta ata gaa ege tat caa 192

Gly lie Ala Pro Asp lie lie Phe Arg Asp Val lie Glu Arg Tyr Gin 50 55 60

aat gaa gtg tet ata act aaa aaa ggc geg ega aat gaa att ata aga 240

Asn Glu Val Ser lie Thr Lys Lys Gly Ala Arg Asn Glu lie lie Arg 65 70 75 80 tta aac ege ttt tta aga tat gat att tet aat ctg tat att cgt gat 288

Leu Asn Arg Phe Leu Arg Tyx Asp lie Ser Asn Leu Tyr lie Arg Asp 85 90 95 tta aga aaa gaa gat ttt gag gag tgg ate aga att ege eta acc gaa 336

Leu Arg Lys Glu Asp Phe Glu Glu Trp He Arg lie Arg Leu Thr Glu 100 105 110 gta teg gat get age gtt aga cgt gag ett gtt act ata teg tea gtg 384

Val Ser Asp Ala Ser Val Arg Arg Glu Leu Val Thr lie Ser Ser Val 115 120 125 ctg aca aca gca ata aat aag tgg gga tat att tea agg cat cca atg 432

Leu Thr Thr Ala lie Asn Lys Trp Gly Tyr lie Ser Arg His Pro Met 130 135 140 act ggt att gaa aaa cca aaa aac teg gca gaa aga aaa gaa ega tat 480

Thr Gly lie Glu Lys Pro Lys Asn Ser Ala Glu Arg Lys Glu Arg Tyr 145 150 155 160 tea gaa cag gac att aaa aca ata tta gaa aca get aga tat tgt gaa 528

Ser Glu Gin Asp lie Lys Thr lie Leu Glu Thr Ala Arg Tyr Cys Glu 165 170 175 gat aaa eta ccc ata aca etc aaa caa aga gta gca att gca 5Ltg tta 576

Asp Lys Leu Pro lie Thr Leu Lys Gin Arg Val Ala lie Ala Met Leu 180 165 190 ttt get att gaa acc get atg cgt get ggt gag att get agt ata aaa 624

Phe Ala lie Glu Thr Ala Met Arg Ala Gly Glu lie Ala Ser lie Lys 195 200 205 egg gat aat gtt ttt ett gaa aag aga ata gta cat Cta ccg aca act 672

Trp Asp Asn Val Phe Leu Glu Lys Arg lie Val His Leu Pro Thr Thr 210 215 220 •210- 136852·序列表.doc

i SI 7201334884 aaa aac ggg cac tct aga gat gtg ccg ctt teg caa aga get gtt geg Lys Asn Giy His Ser Arg Asp Val Pro Leu Ser Gin Arg Ala Val Ala 225 230 235 240 eta att tta aaa atg aaa gag gta gaa aat gga gat ctt gtg ttt cag 768

Leu lie Leu Lys Met Lys Glu Val Glu Asn Gly Asp Leu Val Phe Gin 245 250 255 816 864 912 acc aeg cct gaa tea tta age acc aeg ttt aga gtg tta aag aaa gag Thr Thr Pro Glu Ser Leu Ser Thr Thr Phe Arg Val Leu Lys Lys Glu 260 265 270 tgt gga ctt gaa cat etc cat ttt cat gat aeg aga agg gaa geg ttg Cys Gly Leu Glu His Leu His Phe His Asp Thr Arg Arg Glu Ala Leu 275 280 285 aeg aga tta tct aag aaa gta gat gta atg act eta gcc aaa att age Thr Arg Leu Ser Lys Lys Val Asp Val Met Thr Leu Ala Lys lie Ser 290 295 300 gga cat aga gat tta aga att tta caa aac aca tat tac gca ccg aat 960

Gly His Arg Asp Leu Arg lie Leu Gin Asn Thr Tyr Tyr Ala Pro Asn 305 310 315 320 989 atg agt gaa gtg gca aac ttg ttg gat ta Met Ser Glu Val Ala Asn Leu Leu Asp 325 徉 A 德 e M n 巴 r hr 3 9 白A 3 T 1 3 .张敗 la 1 Λ Λ Λ Λ ΛΑ 0 12 3 ο 1111 ο t 1 2 2 2 2 4 e < < < < <Μ 9 r A r Ty r o el s n s A ly G1 r9 Ar 5 Ly r e s e 5 X 1

Lys Asn Gly Val Arg Lys Ser 20 Asn Ala Trp Ala Val Asp Glu 35 Gly lie Ala Pro Asp lie lie 50 55 Asn Glu Val Ser lie Thr Lys 65 70 Leu Asn Arg Phe Leu Arg Tyr 85 Leu Arg Lys Glu Asp Phe Glu 100

Ala Thr Phe Lys Thr Lys 25

Glu Arg Lys Leu 40 Phe Arg Asp Val

Ala Asp 45 He Glu 60

Ser Glu Ala 30 Leu Ala Lys Arg Tyr Gin

Lys Gly Ala Arg Asn Glu 75 Asp lie Ser Asn Leu Tyr 90 Glu Trp lie Arg lie Arg 105

lie lie Arg 80 lie Arg Asp 95 Leu Thr Glu HO

Val Ser Asp Ala Ser Val Arg 115

Arg Glu Leu Val 120

Thr lie 125

Ser Ser Val His Pro Met Thr Gly lie Glu Lys Pro Lys Asn Ser Ala Glu Arg Lvs Glu Arg Tyr

Leu Thr Thr Ala lie 130

Asn Lys 135

Trp Gly Tyr lie

Ser Arg 140 -211 - 136852·序列表.doc

LSI 1334884

145 150 155 160 5er Glu Gin Asp lie Lys Thr lie Leu Glu Thr Ala Arg Tyr Cys Glu 165 170 175 Asp Lys Leu Pro lie Thr Leu Lys Gin Arg Val Ala lie Ala Met Leu 180 185 190 Phe Ala lie Glu Thr Ala Met Arg Ala Gly Glu lie Ala Ser lie Lys 195 200 205 Trp Asp Asn Val Phe Leu Glu Lys Arg lie Val His Leu Pro Thr Thr 2X0 215 220 Lys Asn Gly His Ser Arg Asp Val Pro Leu Ser Gin Arg Ala Val Ala 225 230 235 240 Leu lie Leu Lys Met Lys Glu Val Glu Asn Gly Asp Leu Val Phe Gin 245 250 255 Thr Thr Pro Glu Ser Leu Ser Thr Thr Phe Arg Val Leu Lys Lys Glu 260 265 270 Cys Gly Leu Glu His Leu His Phe His Asp Thr Arg Arg Glu Ala Leu 275 280 285 Thr Arg Leu Ser Lys Lys Val Asp Val Met Thr Leu Ala Lys lie Ser 290 295 300 Gly His Arg Asp Leu Arg lie Leu Gin Asn Thr Tyr Tyr Ala Pro Asn 305 310 315 320 Met Ser Glu Val Ala Asn Leu Leu Asp 325

<210> 114 <211> 1190 <212> DNA <213>收血忡巴斯谂样铋 <220> <223> SOpE <220> <221> CDS <222> (1) . . (1188) <400> 114 acg tct gaa gaa tat eta cat ggt gtc aaa gtc aca gaa ate aat caa Met Ser Glu Glu Tyr Leu His Gly Val Lys Val Thr Glu He Asn Gin 15 10 15 gca act ege aca att caa agt eta tea acc gca gtc ate ggt att gtc Ala lie Arg Thr lie Gin Ser Leu Ser Thr Ala Val lie Gly He Val 20 25 30 tgc act gca aat gac gca gac aat gaa aca ttc cca etc aat gaa ccc Cys Thr Ala Asn Asp Ala Asp Asn Glu Thr Phe Pro Leu Asn Glu Pro 35 40 45 gtt etc ate aca aac gtg gca geg gca att ggc aag get gga aaa caa Val Leu He Thr Asn Val Ala Ala Ala He Gly Lys Ala Gly Lys Gin 48 96 144 136852-序列表.doc 212- 192 1334884 50 55 60 ggc acg ctt tea cgt geg ett gac ggg att tet gat gta gtc aat tgc 240

Gly Thr Leu Ser Arg Ala Leu Asp Gly lie Ser Asp Val Val Asn Cys 65 70 75 80 aaa gtg att gtt gtg ega gtg caa gaa agt geg caa gaa gac gaa gaa 208

Lys Val lie Val Val Arg Val Gin Glu Ser Ala Gin Glu Asp Glu Glu 85 90 95 aca aaa gca agt gaa atg aac acg gca att att ggc aca ate aca gaa 336

Thr Lys Ala Ser Glu Met Asn Thr Ala lie lie Gly Thr lie Thr Glu 100 105 110 gaa ggg cag tac aca ggc ttg aag geg tta ttg att geg aaa aac aaa 384

Glu Gly Gin Tyr Thr Gly Leu Lys Ala Leu Leu lie Ala Lys Asn Lys 115 120 125 ttc ggt ate aaa cca cgt att tta tgt gtg cca aaa ttc gac aca aaa 432

Phe Gly lie Lys Pro Arg He Leu Cys Val Pro Lys Phe Asp Thr Lys 130 135 140

gaa gtc gcc aca gag ctt gca agt ate gee gee aaa etc aac gca ttt 480

Glu Val Ala Thr Glu Leu Ala Ser lie Ala Ala Lys Leu Asn Ala Phe 145 150 155 160 get tac att tea tgt caa ggg tgt aaa acg aaa gaa caa geg gtg caa 526

Ala Tyr lie Ser Cys Gin Gly Cys Lys Thr Lys Glu Gin Ala Val Gin 165 170 175 tat aaa ege aac ttc tea caa cgt gaa gtc atg ctg ate atg ggc gat 576

Tyr Lys Arg Asn Phe Ser Gin Arg Glu Val Met Leu lie Met Gly Asp 180 185 190 ttt ctg tea ttt aat gtc aac aca tea aaa gtt gag att gac tat gcc 624

Phe Leu Ser Phe Asn Val Asn Thr Ser Lys Val Glu lie Asp Tyr Ala 195 200 205 gtc act cgt geg geg gca atg cgt gca tat ctt gat aaa gaa cag ggc 672

Val Thr Arg Ala Ala Ala Met Arg Ala Tyr Leu Asp Lys Glu Gin Gly 210 215 220 tgg cat acg tet att tea aat aaa ggc att aat ggc gtg age ggt gtc 720

Trp His Thr Ser lie Ser Asn Lys Gly lie Asn Gly Val Ser Gly Val

225 230 235 240 aca caa cca etc tat ttt gac att aac gac age teg act gat gtg aac 768

Thr Gin Pro Leu Tyr Phe Asp He Asn Asp Ser Ser Thr Asp Val Asn 245 250 255 tat etc aat gaa caa ggc ace acg tgt tgc gtg aat cat aat ggc ttt 816

Tyr Leu Asn Glu Gin Gly lie Thr Cys Cys Val Asn His Asn Gly Phe 260 265 270 cgt ttt tgg ggc tta ege acg act gca gaa gat cca tta ttc aag ttt 864

Arg Phe Trp Gly Leu Arg Thr Thr Ala Glu Asp Pro Leu Phe Lys Phe 275 280 285 gaa gtg tac acc ege act gca caa ate tta aaa gat acg att gca ggg 912

Glu Val Tyr Thr Arg Thr Ala Gin lie Leu Lys Asp Thr lie Ala Gly 290 295 300 geg ttt gac tgg gca gtg gat aaa gat att tet gtc acg eta gtg aaa 960 •213- 136852-序列表.doc

I SI 1334884

Ala Phe Asp Trp Ala Val Asp Lys Asp lie Ser Val Thr Leu Val Lys 305 310 315 320 gat att att gaa gca ate aat geg aag tgg cgt gat tac acc aca aaa 1008

Asp lie lie Glu Ala lie Asn Ala Lys Trp Arg Asp Tyr Thr Thr Lys 325 330 335 ggc tac tta att ggc ggt aaa geg tgg ett aat aaa gag ett aac agt 1056

Gly Tyr Leu lie Gly Gly Lys Ala Trp Leu Asn Lys Glu Leu Asn Ser 340 345 350 gca aeg aat tta aaa gat geg aag ttg ttg ate tet tat gat tat cac 1104

Ala Thr Asn Leu Lys Asp Ala Lys Leu Leu lie Ser Tyr Asp Tyr His 355 360 365 cca gta cca ccg etc gaa cag eta ggc ttt aat cag tac att tet gat 1152

Pro Val Pro Pro Leu Glu Gin Leu Gly Phe Asn Gin Tyr lie Ser Asp 370 375 380 gaa tac ett gtt gat ttt tea aat cgt tta gca teg ta 1190

Glu Tyr Leu Val Asp Phe Ser Asn Arg Leu Ala Ser

385 390 395 <210> 115 <211> 396 <212>乐白货 <213>炚血蚀巴M德样1¾ <400> H5

Met Ser Glu Glu Tyr Leu His Gly Val Lys Val Thr Glu lie Asn Gin 15 10 15

Ala lie Arg Thr He Gin Ser Leu Ser Thr Ala Val lie Gly He Val 20 25 30

Cys Thr Ala Asn Asp Ala Asp Asn Glu Thr Phe Pro Leu Asn Glu Pro 35 40 45

Val Leu lie Thr Asn Val Ala Ala Ala lie Gly Lys Ala Gly Lys Gin 50 55 60

Gly Thr Leu Ser Arg Ala Leu Asp Gly lie Ser Asp Val Val Asn Cys

65 70 75 80

Lys Val lie Val Val Arg Val Gin Glu Ser Ala Gin Glu Asp Glu Glu 85 90 95

Thr Lys Ala Ser Glu Met Asn Thr Ala He lie Gly Thr He Thr Glu 100 105 110

Glu Gly Gin Tyr Thr Gly Leu Lys Ala Leu Leu lie Ala Lys Asn Lys 115 120 125

Phe Gly lie Lys Pro Arg lie Leu Cys Val Pro Lys Phe Asp Thr Lys 130 135 140

Glu Val Ala Thr Glu Leu Ala Ser lie Ala Ala Lys Leu Asn Ala Phe 145 150 155 160

Ala Tyr He Ser Cys Gin Gly Cys Lys Thr Lys Glu Gin Ala Val Gin 165 170 175 -214- 136852·序列表.doc 1334884

Tyr Lys Arg Asn Phe Ser Gin Arg Glu Val Met Leu lie Met Gly Asp 180 185 190

Phe Leu Ser Phe Asn Val Asn Thr Ser Lys Val Glu lie Λερ Tyr Ala 195 200 205

Val Thr Arg Ala Ala Ala Met Arg Ala Tyr Leu Asp Lys Glu Gin Gly 210 215 220

Trp His Thr Ser lie Ser Asn Lys Gly lie Asn Gly Val Ser Gly Val 225 230 235 240

Thr Gin Pro Leu Tyr Phe Asp lie Asn Asp Ser Ser Thr Asp Val Asn 245 250 255

Tyr Leu Asn Glu Gin Gly lie Thr Cys Cys Val Asn His Asn Gly Phe 260 265 270

Arg Phe Trp Gly Leu Arg Thr Thr Ala Glu Asp Pro Leu Phe Lys Phe 275 280 285

Glu Val Tyr Thr Arg Thr Ala Gin lie Leu Lys Asp Thr lie Ala Gly 290 295 300

Ala Phe Asp Trp Ala Val Asp Lys Asp He Ser Val Thr Leu Val Lys 305 310 315 320

Asp lie lie Glu Ala lie Asn Ala Lys Trp Arg Asp Tyr Thr Thr Lys 325 330 335

Gly Tyr Leu lie Gly Gly Lys Ala Trp Leu Asn Lys Glu Leu Asn Ser 340 345 350

Ala Thr Asn Leu Lys Asp Ala Lys Leu Leu lie Ser Tyr Asp Tyr His 355 360 365

Pro Val Pro Pro Leu Glu Gin Leu Gly Phe Asn Gin Tyr lie Ser Asp 370 375 380

Glu Tyr Leu Val Asp Phe Ser Asn Arg Leu Ala Ser 385 390 395

<210> 1X6 <211> 2204 <212> DNA <213>收血·:生巴斯谂样逋 <220>

<223> unkK <220>

<221> CDS <222> (1) . . (2202) <400> 116 atg aat aaa aat cgc tat aaa etc att ttt agt aaa act aaa ggc tgt 48

Met Asn Lys Asn Arg Tyr Lys Leu lie Phe Ser Lys Thr Lys Gly Cys 1 5 10 15 ett gta cct gtt get gaa aeg att aat tet gca gta gga aat gcc tea 96

Leu Val Pro Val Ala Glu Thr lie Asn Ser Ala Val Gly Asn Ala Ser 2〇 25 30 •215· 136852-序列表 _doc

i SI 1441334884

tea aaa gac gtt tet gac acc gag ata agt get tet caa cca geg etc Ser Lys Asp Val Ser Asp Thr Glu He Ser Ala Ser Gin Pro Ala Leu 35 40 45 aac teg ccg ett teg acc ett tet gta tta gtc aaa acc gca ttt aat Asn Ser Pro Leu Ser Thr Leu Ser Val Leu Val Lys Thr Ala Phe Asn 50 55 60 ccg gtt tea aca ttg atg teg ttg act tgg aaa gaa tac gee gtt tta Pro Val Ser Thr Leu Met Ser Leu Thr Trp Lys Glu Tyr Ala Val Leu 65 70 75 80 tta tta agt gtg gtg tet ttt cct ett atg gca caa gee tet gat aca Leu Leu Ser Val Val Ser Phe Pro Leu Met Ala Gin Ala Ser Asp Thr 85 90 95 gat tea gtg gta caa aga aaa cct gaa tta act gat gtg aeg aat age Asp Ser Val Val Gin Arg Lys Pro Glu Leu Thr Asp Val Thr Asn Ser 100 105 110 aac age tat cat gtg gaa tta gat aga gag cat cat aaa ggg gag cat Asn Ser Tyr His Val Glu Leu Asp Arg Glu His His Lys Gly Glu His 115 120 125 caa aca aaa ate aaa cat act gag aat aat gtc ate att gtt gat att Gin Thr Lys He Lys His Thr Glu Asn Asn Val lie lie Val Asp lie 130 135 140 gca aaa cca aac caa aag ggc att tea gat aac cgt ttt aaa cac ttc Ala Lys Pro Asn Gin Lys Gly lie Ser Asp Asn Arg Phe Lys His Phe 145 150 155 160 aac ate cca aat ggg geg gta ttt aac aat age gee aag gaa aaa ege Asn lie Pro Asn Gly Ala Val Phe Asn Asn Ser Ala Lys Glu Lys Arg 165 170 175 tea cag tta gtg ggg tat ttg cca ggt aac cag aat tta aeg gaa ggt Ser Gin Leu Val Gly Tyr Leu Pro Gly Asn Gin Asn Leu Thr Glu Gly 180 185 190 agt gaa gca aaa geg ate tta aat cag gtg act gga ccg gat gee agt Ser Glu Ala Lys Ala He Leu Asn Gin Val Thr Gly Pro Asp Ala Ser 195 200 205 aaa att gaa ggc gee ett gaa att tta ggg caa aaa gee gat ttg gtg Lys lie Glu Gly Ala Leu Glu lie Leu Gly Gin Lys Ala Asp Leu Val 210 215 220 att geg aac caa aat ggc att gtg ett aat ggg gta aaa acc att aat lie Ala Asn Gin Asn Gly lie Val Leu Asn Gly Val Lys Thr lie Asn 225 230 235 240 gee aat cgt ttt gtg gca aca acc agt agt acc att gat cct gag caa Ala Asn Arg Phe Val Ala Thr Thr Ser Ser Thr lie Asp Pro Glu Gin 245 250 255 atg cag eta aat gtc aeg caa ggt aca gtg aca att ggg gtg gat gga Met Gin Leu Asn Val Thr Gin Gly Thr Val Thr lie Gly Val Asp Gly 260 265 270 tet gee aca gat ggc tta cct tac ttg gat acc att gee aaa aag att Phe Ala Thr Asp Gly Leu Pro Tyr Leu Asp lie He Ala Lys Lys lie 275 280 285 192 240 286 336 3Θ4 432 480 528 576 624 672 720 768 816 136852·序列表.doc -216- 864 1334884

gaa caa aaa caa geg att aca aaa gaa aga aca gga aat tee gaa acc 912 Glu Gin 290 Lys Gin Ala lie Thr 295 Lys Glu Arg Thr Gly Asn 300 Ser Glu Thr gat ate act ttt gtc gca ggt aac agt aaa tat gat tta aag aca cat 960 Asp 305 lie Thr Phe Val Ala 310 Gly Asn Ser Lys Tyr 315 Asp I»eu Lys Thr His 320 caa gtg aca gaa aag cat acc get gag gca caa ggt gaa att geg att 1008 Gin Val Thr Glu Lys 325 His Thr Ala Glu Ala 330 Gin Gly Glu lie Ala 335 lie age ggt geg agt acc ggt gca atg tac ggt aaa aat ate aaa tta ate 1056 Ser Gly Ala Ser 340 Thr Gly Ala Met Tyr 345 Gly Lys Asn lie Lys 350 Leu lie gta aeg gat aaa ggc get ggg gta aaa cat gat ggc att att tta tet 1104 Val Thr Asp 355 Lys Gly Ala Gly Val 360 Lys His Asp Gly lie 365 lie Leu Ser gag geg gat att caa att gaa acc cat gag ggc gat gtt gaa tta ggc 1152 Glu Ala 370 Asp lie Gin lie Glu 375 Thr His Glu Gly Asp 380 Val Glu Leu Gly aat aca aaa aat aat cag aat gag aat tat gee aaa get cat geg gaa 1200 Asn 385 Thr Lys Asn Asn Gin 390 Asn Glu Asn Tyr Ala 395 Lys Ala His Ala Glu 400 ggg aat ttt aeg gtt aaa ggc ggt aag cac gtt att att ggt aag gaa 1248 Gly Asn Phe Thr Val 405 Lys Gly Gly Lys His 410 Val lie lie Gly Lys 415 Glu gtt aaa gee aac aaa geg gtc gat att caa gca caa gaa aca aca gta 1296 Val Lys Ala Asn 420 Lys Ala Val Asp lie 425 Gin Ala Gin Glu Thr 430 Thr Val aga caa aat geg aaa tta act gee aaa aeg agt gee aaa att aca 1344 Arg Gin Asn 435 Ala Lys Leu Thr Ala 440 Lys Thr Ser Ala Lys 445 lie Thr Ala agt aag agt gtg aat ett gaa gat aac geg aaa ett att get aat gag 1392 Ser Lys 450 Ser Val Asn Leu Glu 455 Asp Asn Ala Lys Leu 460 lie Ala Asn Glu ctg age aca aca acc aat aaa tta acc aat aaa ggt age att tac ggc 1440 Leu 465 Ser Thr Thr Thr Asn 470 Lys Leu Thr Asn Lys 475 Gly Ser lie Tyr Gly 4B0 aag aaa gtg aeg eta gat get gat aat tta gtc aat agt aaa gaa ate 1488 Lys Lys Val Thr Leu 4Θ5 Asp Ala Asp Asn Leu 490 Val Asn Ser Lys Glu 495 lie tat geg tet age gaa ett gat att caa acc aaa ggt cgt gat ett tta 1536 Tyr Ala Ser Ser 500 Glu Leu Asp lie Gin 505 Thr hys Gly Arg Asp 510 Leu Leu ett gag gat ggg gtt aac caa cca ctg agt ttc tta aaa ggc get tea 1584 Leu Glu Asp 515 Gly Val Asn Gin Pro 520 Leu Ser Phe Leu Lys 525 Gly Ala Ser ttg tta geg ccg ggg ttt gtc aac act 999 eta att cac agt aac ggt 1632 Leu Leu 530 Ala Pro Gly Phe Val 535 Asn Thr Gly Leu lie 540 His Ser Asn Gly 13682-序列表.doc • 217- ISi 1334884 aat gcc aag etc act ttt aaa gat gac acc agt ttt gtg act gaa gga 16B0

Asn Ala Lys Leu Thr Phe Lys Asp Asp Thr Ser Phe Val Thr Glu Gly 545 550 555 560 aat aac ttt ate aca gca aaa gac aac tta gaa ate aeg gca aaa aat 1728

Asn Asn Phe He Thr Ala Lys Asp Asn Leu Glu lie Thr Ala Lys Asn 565 570 575 gtt caa att gat caa geg aaa aat att caa tta aac geg aat ate aeg 1776

Val Gin lie Asp Gin Ala Lys Asn lie Gin Leu Asn Ala Asn lie Thr 580 585 590 ate aat acc aag tet ggt ttt gtg aat tac ggt acc tta gca agt get 1824 lie Asn Thr Lys Ser Gly Phe Val Asn Tyr Gly Thr Leu Ala Ser Ala 595 600 605 caa aat tta aeg att aat acc gaa caa ggc age att tat aac ata ggc 1872

Gin Asn Leu Thr lie Asn Thr Glu Gin Gly Ser lie Tyr Asn lie Gly 610 615 620 ggt ate ttg ggg geg ggt aaa agt ttg aat ctg age geg aaa aga gga 1920

Gly lie Leu Gly Ala Gly Lys Ser Leu Asn Leu Ser Ala Lys Arg Gly 625 630 635 640 gaa aac caa gga gga tat ett att aat caa ggt aag agt eta etc cat 1968

Glu Asn Gin Gly Gly Tyr Leu lie Asn Gin Gly Lys Ser Leu Leu His 645 650 655 tet gaa ggc gcc atg aac etc aca geg gat ege aeg gtg tac aat tta 2016

Ser Glu Gly Ala Met Asn Leu Thr Ala Asp Arg Thr Val Tyr Asn Leu 660 665 670 ggg aat att ttt get aaa ggt gac geg aeg ate aat gca aac geg tta 2064

Gly Asn He Phe Ala Lys Gly Asp Ala Thr lie Asn Ala Asn Ala Leu 675 680 685 att aat gat gtt act etc aca ggt cgt ett gag tat caa gat ctg aaa 2X12 lie Asn Asp Val Thr Leu Thr Gly Arg Leu Glu Tyr Gin Asp Leu Lys 690 695 700 aaa gat tat aeg cgt tat tat cgt ate aat gaa aeg gca aaa cat ggt 2160

Lys Asp Tyr Thr Arg Tyr Tyr Arg lie Asn Glu Thr Ala Lys His Gly 705 710 715 720

tgg cat aat aac ttc tat gaa tta aac gtc gac aga gtt tet tg 2204

Trp His Asn Asn Phe Tyr Glu Leu Asn Val Asp Arg Val Ser 725 730 <210> 117 <21l> 734 <212>货白货 <213>收血作巴斯谂样餡 <400> 117

Met Asn Lys Asn Arg Tyr Lys Leu lie Phe Ser Lys Thr Lys Gly Cys IS 10 15

Leu Val Pro Val Ala Glu Thr lie Asn Ser Ala Val Gly Asn Ala Ser 20 25 30

Ser Lys Asp Val Ser Asp Thr Glu lie Ser Ala Ser Gin Pro Ala Leu 35 40 45 •218· 136852·序列表.doc i si 1334884

Asn Ser Pro Leu Ser Thr Leu Ser Val Leu Val Lys Thr Ala Phe Asn 50 55 60

Pro Val Ser Thr Leu Met Ser Leu Thr Trp Lys Glu Tyr Ala Val Leu 65 70 75 80

Leu Leu Ser Val Val Ser Phe Pro Leu Met Ala Gin Ala Ser Asp Thr 85 90 95

Asp Ser Val Val Gin Arg Lys Pro Glu Leu Thr Asp Val Thr Asn Ser 100 105 110

Asn Ser Tyr His Val Glu Leu Asp Arg Glu His His Lys Gly Glu His 115 120 125

Gin Thr Lys He Lys His Thr Glu Asn Asn Val lie lie Val Asp lie 130 135 140

Ala Lys Pro Asn Gin Lys Gly lie Ser Asp Asn Arg Phe Lys His Phe 145 150 155 160

Asn lie Pro Asn Gly Ala Val Phe Asn Asn Ser Ala Lye Glu Lys Arg 165 170 175

Ser Gin Leu Val Gly Tyr Leu Pro Gly Asn Gin Asn Leu Thr Glu Gly 180 165 190

Ser Glu Ala Lys Ala He Leu Asn Gin Val Thr Gly Pro Asp Ala Ser 195 200 205

Lys lie Glu Gly Ala Leu Glu lie Leu Gly Gin Lys Ala Asp Leu Val 210 215 220 lie Ala Asn Gin Asn Gly lie Val Leu Asn Gly Val Lys Thr lie Asn 225 230 235 240

Ala Asn Arg Phe Val Ala Thr Thr Ser Ser Thr lie Asp Pro Glu Gin 245 250 255

Met Gin Leu Asn Val Thr Gin Gly Thr Val Thr lie Gly Val Asp Gly 260 265 270

Phe Ala Thr Asp Gly Leu Pro Tyr Leu Asp lie lie Ala Lys Lys lie 275 280 285

Glu Gin Lys Gin Ala He Thr Lys Glu Arg Thr Gly Asn Ser Glu Thr 290 295 300

Asp lie Thr Phe Val Ala Gly Asn Ser Lys Tyr Asp Leu Lys Thr His 305 310 315 320

Gin Val Thr Glu Lys His Thr Ala Glu Ala Gin Gly Glu lie Ala lie 325 330 335

Ser Gly Ala Ser Thr Gly Ala Met Tyr Gly Lys Asn lie Lys Leu He 340 345 350

Val Thr Asp Lys Gly Ala Gly Val Lys His Asp Gly lie lie Leu Ser 355 360 365

Glu Ala Asp lie Gin lie Glu Thr His Glu Gly Asp Val Glu Leu Gly 370 375 360 136852-序列表.doc -219-

1 SI 1334884

Asn Thr Lys Asn Asn Gin Asn Glu Asn Tyr Ala Lys Ala His Ala Glu 385 390 395 400

Gly Asn Phe Thr Val Lys Gly Gly Lys His val lie lie Gly Lys Glu 405 410 415

Val Lys Ala Asn Lys Ala Val Asp lie Gin Ala Gin Glu Thr Thr Val 420 425 430

Arg Gin Asn Ala Lys Leu Thr Ala Lys Thr Ser Ala Lys lie Thr Ala 435 440 445

Ser Lys Ser Val Asn Leu Glu Asp Asn Ala Lys Leu lie Ala Asn Glu 450 455 460

Leu Ser Thr Thr Thr Asn Lys Leu Thr Asn Lys Gly Ser lie Tyr Gly 465 470 475 480

Lys Lys Val Thr Leu Asp Ala Asp Asn Leu Val Asn Ser Lys Glu lie 485 490 495

Tyr Ala Ser Ser Glu Leu Asp He Gin Thr Lys Gly Arg Asp Leu Leu 500 505 510

Leu Glu Asp Gly Val Asn Gin Pro Leu Ser Phe Leu Lys Gly Ala Ser 515 520 525

Leu Leu Ala Pro Gly Phe Val Asn Thr Gly Leu lie His Ser Asn Gly 530 535 540

Asn Ala Lys Leu Thr Phe Lys Asp Asp Thr Ser Phe Val Thr Glu Gly 545 550 555 560

Asn Asn Phe lie Thr Ala Lys Asp Asn Leu Glu lie Thr Ala Lys Asn 565 570 575

Val Gin lie Asp Gin Ala Lys Asn lie Gin Leu Asn Ala Asn lie Thr 580 585 590 lie Asn Thr Lys Ser Gly Phe Val Asn Tyr Gly Thr Leu Ala Ser Ala 595 600 605

Gin Asn Leu Thr lie Asn Thr Glu Gin Gly Ser lie Tyr Asn lie Gly 610 615 620

Gly lie Leu Gly Ala Gly Lys Ser Leu Asn Leu Ser Ala Lys Arg Gly 625 630 635 640

Glu Asn Gin Gly Gly Tyr Leu lie Asn Gin Gly Lys Ser Leu Leu His 645 650 655

Ser Glu Gly Ala Met Asn Leu Thr Ala Asp Arg Thr Val Tyr Asn Leu 660 665 670

Gly Asn lie Phe Ala Lys Gly Asp Ala Thr lie Asn Ala Asri Ala Leu 675 680 685 lie Asn Asp Val Thr Leu Thr Gly Arg Leu Glu Tyr Gin Asp Leu Lys 690 695 700

Lys Asp Tyr Thr Arg Tyr Tyr Arg lie Asn Glu Thr Ala Lys His Gly 705 710 715 720 -220· 136852_ 序列表.doc ISi 1334884

Trp His Asn Asn Phe Tyr Glu Leu Asn Val Asp Arg Val Se'r 725 730

<210> 118 <211> 251 <212> DNA <213 >欧血性巴斯德、样菌 — <220>

<223> unkO <220> <221> CDS <222> (1)..(249) <400> 118 atg aaa att act att aca cga aat cat cca gaa gta ttt caa gaa tcc 48

Met Lys lie Thr 工le Thr Arg Asn His Pro Glu Val Phe Gin Glu Ser 1 5 10 15

get cgt tta gta gcc gaa aag ttc att aaa gec caa tgt gta gaa gca 96

Ala Arg Leu Val Ala Glu Lys Phe lie Lys Ala Gin Cys Val Glu Ala 20 25 30 tta aca ttg get ttg att gag ggt gtc gag cac ttt gtg ctg gaa ggt 144

Leu Thr Leu Ala Leu lie Glu Gly Val Glu His Phe Val Leu Glu Gly 35 40 45 gag gag gaa age aaa agg gga cat agt att aag gtt gta tta aaa gga 192

Glu Glu Glu Ser Lys Arg Gly His Ser lie Lys Val Val Leu Lys Gly 50 55 60 agt cac gaa gtt att aag tea gag gtg aat aca aat gaa aaa aat cat 240

Ser His Glu Val lie Lys Ser Glu Val Asn Thr Asn Glu Lys Asn His 65 70 75 80 tgt aat cat ta 251

Cys Asn His

<210> 119 <211> 83 <212>蛋白甘 c213>欧血性巴則谂桿谪 <400> 119

Met Lys lie Thr He Thr Arg Asn His Pro Glu Val Phe Gin Glu Ser 1 5 10 15

Ala Arg Leu Val Ala Glu Lys Phe He Lys Ala Gin Cys Val Glu Ala 20 25 30

Leu Thr Leu Ala Leu lie Glu Gly Val Glu His Phe Val Leu Glu Gly 35 40 45

Glu Glu Glu Ser Lys Arg Gly His Ser lie Lys Val Val Leu Lys Gly 50 55 60

Ser His Glu Val lie Lys Ser Glu Val Asn Thr Asn Glu Lys Asn His 65 70 75 80 221 - 136852-序列表.doc IS1 1334884

Cys Asn His atg cgt gca tat ctt gat aaa gaa cag ggc tgg cat acg tct att tea 48

Met Arg Ala Tyr Leu Asp Lys Glu Gin Gly Trp His Thr Ser He Ser 15 10 15 aat aaa ggc att aat ggc gtg age ggt gtc aca caa cca etc tat ttt 96

Asn Lys Gly lie Asn Giy Val Ser Gly Val Thr Gin Pro Leu Tyr Phe 20 25 30 gac att aac gac age teg act gat gtg aac tat etc aat gaa caa ggc 144

Asp lie Asn Asp Ser Ser Thr Asp Val Asn Tyr Leu Asn Glu Gin Gly 35 40 45 ate acg tgt tgc gtg aat cat aat ggc ttt cgt ttt tgg ggc tta ege 192 lie Thr Cys Cys Val Asn His Asn Gly Phe Arg Phe Trp Gly Leu Arg 50 55 60 acg act gca gaa gat cca tta ttc aag ttt gaa gtg tac acc ege act 240

Thr Thr Ala Glu Asp Pro Leu Phe Lys Phe Glu Val Tyr Thr Arg Thr 65 70 75 80 gca caa ate tta aaa gat acg att gca ggg geg ttt gat tgg gca gtg 288

Ala Gin lie Leu Lys Asp Thr He Ala Gly Ala Phe Asp Trp Ala Val 85 90 95 gat aaa gac att tct gtc acg eta gtg aaa gat att att gaa gca ate 336

Asp Lys Asp lie Ser Val Thr Leu Val Lys Asp lie lie Glu Ala lie 100 105 110

<210> 120 <211> 548 <212> DNA <213> 畋血，1生匕斯德桿涵 <220> <223> unkP <220> <221> CDS <222> (1) . . (546) <4 00> 120 aat geg aag tgg cgt gat tac acc aca aaa ggc tac tta att ggc ggt 384

Asn Ala Lys Trp Arg Asp Tyr Thr Thr Lys Gly Tyr Leu lie Gly Gly 115 120 125 aaa geg tgg ctt aat aaa gag ctt aac agt gca acg aat tta aaa gat 432

Lys Ala Trp Leu Asn Lys Glu Leu Asn Ser Ala Thr Asn Leu Lys Asp 130 135 140 geg aag ttg ttg ate tct tat gat tat cac cca gta cca ccg etc gaa 480

Ala Lys Leu Leu lie Ser Tyr Asp Tyr His Pro Val Pro Pro Leu Glu 145 150 155 160 cag eta ggc ttc aat cag tac att tct gat gaa tac ctt gtt gat ttt 528

Gin Leu Gly Phe Asn Gin Tyr lie Ser Asp Glu Tyr Leu Val Asp Phe 16S 170 175 tea aat cgt tta gca teg ta 548 • 222· 136852·序列表.doc 1334884

Ser Asn Arg Leu Ala Ser 180 <2X0> 121 <211> 182 <212>蛋白竹 <213> <400> 121

Met Arg Ala Tyr Leu Asp Lys Glu Gin Gly Trp His Thr Ser lie Ser 1 5 10 15

Asn Lys Gly lie Asn Gly Val Ser Gly Val Thr Gin Pro Leu Tyr Phe 20 25 30

Asp lie Asn Asp Ser Ser Thr Asp Val Asn Tyr Leu Asn Glu Gin Gly 35 40 45 lie Thr Cys Cys val Asn His Asn Gly Phe Arg Phe Trp Gly Leu Arg 50 55 60

Thr Thr Ala Glu Asp Pro Leu Phe Lys Phe Glu Val Tyr Thr Arg Thr 65 70 75 80

Ala Gin He Leu Lys Asp Thr lie Ala Gly Ala Phe Asp Trp Ala Val 85 90 95

Asp Lys Asp lie Ser Val Thr Leu Val Lys Asp lie lie Glu Ala lie 100 105 110

Asn Ala Lys Trp Arg Asp Tyr Thr Thr Lys Gly Tyr Leu lie Gly Gly 115 120 125

Lys Ala Trp Leu Asn Lys Glu Leu Asn Ser Ala Thr Asn Leu Lys Asp 130 135 140

Ala Lys Leu Leu lie Ser Tyr Asp Tyr His Pro Val Pro Pro Leu Glu 145 150 155 160

Gin Leu Gly Phe Asn Gin Tyr He Ser Asp Glu Tyr Leu Val Asp Phe 165 170 175

Ser Asn Arg Leu Ala Ser 180

<210> 122 <211> 69 <212> DNA <213>钩哚肿夂放綿涔菡 <220> <223> apvA-orl <220> <221> CDS <222> (1)..(69) <400> 122 atg ctt tat gtc atg etc gcc aat agg aeg tet ata att tea tea ate 48

Met Phe Tyr Val Met Leu Ala Asn Arg Thr Ser lie lie Ser Ser lie 15 10 15 -223 - 136852·序列表.doc i si 1334884 gat aag ttt aag ata etc age Asp Lys Phe Lys He Leu Ser 20 <210> 123 <211> 23 <2X2> <213> I构膜肺炎故焯样逋 <400> 123

Met Phe Tyr Val Met Leu Ala Asn Arg Thr Ser He He Ser Ser He 15 10 15

Asp Lys Phe Lys lie Leu Ser 20

<210> 124 <211> 64 <2X2> DNA

<213>钩祺肺炎放绵样闺 <220> <223> apvA-or2 <220> <221> CDS <222> (3)..(62) <400> 124 ag eta agt ate tta aac tta tc9 att gat gaa att ata gac gtc eta Leu Ser He Leu Asn ben Ser lie Asp Glu lie lie Asp Val Leu 15 l〇 15 ttg gca age atg aca ta Leu Ala Ser Met Thr 20

<21〇> 125 <211> 20 <212>炎白甘 <213:>胸膜胂炎放絲桴菌 <400> 125

Leu Ser lie Leu Asn Leu Ser lie Asp Glu lie lie Asp Val Leu Leu 1 5 10 15

Ala Ser Met Thr 20

<210> 126 <211> 653 <212> DNA <213>驹说肿.欠放绵样l/j <220>

<223> apvB <220>

<221> CDS -224- 136852-序列表.doc 1334884

<222> (1).. (651) <400> 126 tta att age ttt cct ttt att act ttt gca agt aat gtt aat gga gcc 48 Leu He Ser Phe Pro Phe lie Thr Phe Ala Ser Asn Val Asn Gly Ala 1 5 10 15 gaa att gga ttg gga gga gcc cgt gag agt agt att tac tat tet aaa 96 Glu lie Gly Leu Gly Gly Ala Arg GlU Ser Ser lie Tyr Tyr Ser Lys 20 25 30 cat aaa gta gca aca aat ccc ttt tta gca ett gat ett tet tta ggt 144 His Lys Val Ala Thr Asn Pro Phe Leu Ala Leu Asp Leu Ser Leu Gly 35 40 45 aat ttt tat atg aga ggg act gca gga att age gaa ata gga tat gaa 192 Asn Phe Tyr Met Arg Gly Thr Ala Gly lie Ser Glu lie Gly Tyr Glu 50 55 60 caa tet ttc act gac aat ttc age gta tea ctg ttt gtt aac cca ttt 240 Gin Ser Phe Thr Asp Asn Phe Ser Val Ser Leu Phe Val Asn Pro Phe 65 70 75 80 gat ggt ttt tea att aaa gga aaa gac ttg tta cct gga tat caa agt 286 Asp (Sly Phe Ser lie Lys Gly Lys Asp Leu Leu Pro Gly Tyr Gin Ser 85 90 95 att caa act ege aaa act caa ttt gcc ttt ggt tgg gga tta aat tat 336 lie Gin Thr Arg Lys Thr Gin Phe Ala Phe Gly Trp Gly Leu Asn Tyr 100 105 110 aat ttg gga ggt tta ttc ggc Cta aat gat act ttt ata tcc ttg gaa 384 Asn Leu Gly Gly Leu Phe Gly Leu Asn Asp Thr Phe He Ser Leu Glu 115 120 125 gga aaa age gga aaa cgt ggt geg agt agt aat gtc age tta ett aaa 432 Gly Lys Ser Gly Lys Arg Gly Ala Ser Ser Asn Val Ser Leu Leu Lys 130 135 140 teg ttt aat atg aeg aaa aat tgg aaa gtt tea cca tat att ggc tea 480 Ser Phe Asn Met Thr Lys Asn Trp Lys Val Ser Pro Tyr lie Gly Sar 145 150 155 160 agt tat tat tea tet aaa tat aca gat tat tac ttt ggt att aaa caa 528 Ser Tyr Tyr Ser Ser Lys Tyr Thr Asp Tyr Tyr Phe Gly lie Lys Gin 165 170 175 tcc gaa tta ggt aat aaa att aca tcc gta tat aaa cct aaa gca get 576 Ser Glu Leu Gly Asn Lys lie Thr Ser Val Tyr Lys Pro Lys Ala Ala 180 185 190 tat gca aca cac ata ggt att aat act gat tat get ttc aeg aac aat 624 Tyr Ala Thr His He Gly lie Asn Thr Asp Tyr Ala Phe Thr Asn Asn 195 200 205 ett ggc atg ggt tta tet gtc ggt tgg at 653 Leu Gly Met Gly Leu Ser Val Gly Trp 210 215 <210> 127 <211> 217 <212>乐白货 136852·序列表.doc -225 - 1334884 <213>胸躲胂災放線徉谪 <400> 127

Leu lie Ser Phe Pro Phe lie Thr Phe Ala Ser Asn Val Asn Gly Ala 1 5 10 15

Glu He Gly Leu Gly Gly Ala Arg Glu Ser Ser lie Tyr Tyr Ser Lys 20 25 30

His Lys Val Ala Thr Asn Dro Phe Leu Ala Leu Asp Leu Ser Leu Gly 35 40 45

Asn Phe Tyr Met Arg Gly Thr Ala Gly lie Ser Glu lie Gly Tyr Glu 50 55 60

Gin Ser Phe Thr Asp Asn Phe Ser Val Ser Leu Phe Val Asn Pro Phe 65 70 75 80

Asp Gly Phe Ser lie Lys Gly Lys Asp Leu Leu Pro Gly Tyr Gin Ser 85 90 * 95

lie Gin Thr Arg Lys Thr Gin Phe Ala Phe Gly Trp Gly Leu Asn Tyr 100 105 110

Asn Leu Gly Gly Leu Phe Gly Leu Asn Asp Thr Phe lie Ser Leu Glu 115 120 125

Gly Lys Ser Gly Lys Arg Gly Ala Ser Ser Asn Val Ser Leu Leu Lys 130 135 140

Ser Phe Asn Met Thr Lys Asn Trp Lys Val Ser Pro Tyr lie Gly Ser 145 150 155 160

Ser Tyr Tyr Ser Ser Lys Tyr Thr Asp Tyr Tyr Phe Gly lie Lys Gin 165 170 175

Ser Glu Leu Gly Asn Lys He Thr Ser Val Tyr Lys Pro Lys Ala Ala 180 185 190

Tyr Ala Thr His lie Gly He Asn Thr Asp Tyr Ala Phe Thr Asn Asn 195 200 205

Leu Gly Met Gly Leu Ser Val Gly Trp

210 215

<210> 128 <211> 242 <212> DNA <213>胸膜胂.¾¾綿样逋 <220> <223> apvC <220>

<221> CDS <222> (1)..(240) <400> 128 atg tgg egg atg gga gat ttt atg tet aaa aaa gag agg ctg aat gat 48

Met Trp Arg Met Gly Asp Phe Met Ser Lys Lys Glu Arg Leu Asn Asp 1 5 10 15 • 226- 136852·序列表.doc 1 51 1334884 atg get ege cag att tta tea geg geg gag ttg etc att gca aag gaa 96 Met Ala Arg Gin lie Leu Ser Ala Ala Glu Leu Leu lie Ala Lys Glu 20 25 30 ggt ttg caa aat tta teg atg agg aaa ate gca agt gaa gee ggt ate 144 Gly Leu Gin Asn Leu Ser Met Arg Lys lie Ala Ser Glu Ala Gly lie 35 40 45 gca aca ggc aeg ett tat etc tat ttc aaa aeg aaa gac gag tta ctg 192 Ala Thr Gly Thr Leu Tyr Leu Tx Phe Lys Thr Lys Asp Glu Leu Leu 50 55 60 gat tgt ttg geg gaa caa tta cat gaa ega tat tat cgt tat ctg aat 240 Asp Cys Leu Ala Glu Gin Leu His Glu Axg Tyr Tyr Arg Tyr Leu Asn 65 70 75 80 at 242

<210> 129 <211> 80 <212> ·货白甘 <213>胸膜胂炎放綿样菌 <400> 129 Met Trp Arg Met Gly Asp Phe Met Ser Lys Lys Glu Arg Leu Asn Asp 15 10 15 Met Ala Arg Gin lie Leu Ser Ala Ala Glu Leu Leu He Ala Lys Glu 20 25 30 Gly Leu Gin Asn Leu Ser Met Arg Lys lie Ala Ser Glu Ala Gly lie 35 40 45 Ala Thr Gly Thr Leu Tyr Leu Tyr Phe Lys Thr Lys Asp Glu Leu Leu 50 55 60 Asp Cys Leu Ala Glu Gin Leu His Glu Arg Tyr Tyr Arg Tyr Leu Asn 65 70 75 80

<210> 130 <211> 527 <212> DNA <213>胸膜胂炎放竦样菡 <220> <223> apvD <220> <221> CDS <222> (1)..(525) <400> 130 aat att caa aaa aca gtt att get age ggc aca ttg caa geg act gaa Asn lie Gin Lys Thr Val lie Ala Ser Gly Thr Leu Gin Ala Thr Glu 15 10 15 48 caa gta gat act ggt gca caa gta tet ggg cag att aag cat att tta Gin Val Asp lie Gly Ala Gin Val Ser Gly Glr： lie Lys His lie Leu 20 25 30 96 gta caa gaa gga cag aag gtt aaa aaa ggt gag eta tta get gta att 144 136852·序列表.doc 227- 1 ] 1334884

<400> 131

Val Gin Glu 35 Gly Gin Lys Val Lys 40 hys Gly Glu Leu Leu 45 Ala Val lie gat cca cgt ctg get gaa aeg gaa tta aaa eta gca aaa get gag eta 192 Asp Pro 50 Arg Leu Ala Glu Thr 55 Glu Leu Lys Leu Ala 60 Lys Ala Glu Leu gca aat get tet get aat ttg gat aca aaa aaa att aat ett aag caa 240 Ala 65 Asn Ala Ser Ala Asn 70 Leu Asp Thr Lys Lys 75 lie Asn Leu Lys Gin 80 ctg caa tea gat tgg gaa cgt cat caa cgt ttg ata ega acc aat geg 288 Leu Gin Ser Asp Trp 85 Glu Arg His Gin Arg 90 Leu lie Arg Thr Asn 95 Ala aca age caa aag gaa aca gaa gaa gca aaa agt aga tta aat aeg gcc 336 Thr Ser Gin Lys 100 Glu Thr Glu Glu Ala 105 Lys Ser Arg Leu Asn 110 Thr Ala aaa gca gaa ett caa att geg caa aat aat eta gat ate get aaa ate 384 Lys Ala Glu 115 Leu Gin lie Ala Gin 120 Asn Asn Leu Asp lie 125 Ala Lys lie aga gtg gaa aaa get gaa acc gaa eta gga tat aca gaa att cgt tet 432 Arg Val 130 Glu Lys Ala Glu Thr 135 Glu Leu Gly Tyr Thr 140 Glu lie Arg Ser cca ett gat gca aca gta att tea gta ttt geg caa aat ggt caa act 480 Pro 145 Leu Asp Ala Thr Val ISO lie Ser Val Phe Ala 155 Gin Asn Gly Gin Thr 160 tta gtc acc acc caa Leu Val Thr Thr Gin 165 <210> 131 <211> 175 <212>蛋白背 c213:>胸膜肺炎放線桴菌 caa Gin gta Val cca Pro gtg Val ctg Leu 170 atg Met aaa Lys tta Leu get Ala aat Asn 175 at 527

Asn lie Gin Lys Thr Val lie Ala Ser Gly Thr Leu Gin Ala Thr Glu 15 10 15

Gin Val Asp He Gly Ala Gin Val Ser Gly Gin lie Lys His lie Leu 20 25 30

Val Gin Glu Gly Gin Lys Val Lys Lys Gly Glu Leu Leu Ala Val lie 35 40 45

Asp Pro Arg Leu Ala Glu Thr Glu Leu Lys Leu Ala Lys Ala Glu Leu 50 55 60

Ala Asn Ala Ser Ala Asn L»eu Asp Thr Lys Lys lie Asn Leu Lys Gin 65 70 75 80

Leu Gin Ser Asp Trp Glu Arg His Cln Arg Leu lie Arg Thr Asn Ala 85 90 95

Thr Ser Gin Lys Glu Thr Glu Glu Ala Lys Ser Arg Leu Asn Thr Ala 100 105 110 228· Π6852·序列表.doc

1334884

Lys Ala Glu Leu Gin lie Ala Gin Asn Asn Leu Asp lie Ala Lys lie 115 120 125

Arg Val Glu Lys Ala Glu Thr Glu Leu Gly Tyr Thr Glu lie Arg Ser 130 135 140

Pro Leu Asp Ala Thr Val lie Ser Val Phe Ala Gin Asn Gly Gin Thr 145 150 155 ISO

Leu Val Thr Thr Gin Gin Val Pro Val Leu Mer Lys Leu Ala Asn 165 170 175

<210> 132 <211> 867 <212> DNA <213>胸膜肺.¾放绵桴滴 <220>

<223> atpG

<220> <221> CDS <222> (1)..(864) <400> 132 atg gc|L ggt gcg aaa gag ata aga acc aaa att gca agt gtg aaa aat 48

Met All Gly Ala Lys Glu lie Arg Thr Lys lie Ala Ser Val Lys Asn 15 10 15 act caa aaa ate acc aaa gca atg gaa atg gtt get acc tet aaa atg 96

Thr Gin Lys lie Thr Lys Ala Met Glu Met Val Ala Thr Ser Lys Met 20 25 30 cgt aaa aeg caa gag cgt atg get gcc agt cgt cct tat teg gaa aca 144

Arg Lys Thr Gin Glu Arg Met Ala Ala Ser Arg Pro Tyr Ser Glu Thr 35 40 45 ate cgt aag gtg att age cat att geg aaa gga age att ggt tat aag 192 lie Arg Lys Val lie Ser His lie Ala Lys Gly Ser lie Gly Tyr Lys 50 55 60 cac ccg ttt tta act gaa cgt gat att aaa aaa gta ggc tat ett gtc 240

His Pro Phe Leu Thr Glu Arg Asp lie Lys Lys Val Gly Tyr Leu Val 65 70 75 80 gtt teg acc gat ege ggt tta tgc ggt ggc ett aat ate aat tta ttc 288

Val Ser Thr Asp Arg Gly Leu Cys Gly Gly Leu Asn lie Asn Leu Phe 85 90 95 aaa geg act ttg aat gaa ttt aaa aeg tgg aaa gat aaa gac gtt agt 336

Lys Ala Thr Leu Asn Glu Phe Lys Thr Trp Lys Asp Lys Asp Val Ser 100 105 * 110 gtt gag ett ggt tta gta ggg teg aaa ggc gta age ttt tac caa aat 384

Val Glu Leu Gly Leu Val Gly Ser Lys Gly Val Ser Phe Tyr Gin Asn 115 120 125 eta ggc tta aac gtg aga tet caa gta aeg gga tta ggc gat aat ccg 432

Leu Gly Leu Asn Val Arg Ser Gin Val Thr Gly Leu Gly Asp Asn Pro 130 135 140 gaa atg gaa cgt ate gtg ggc gca gtt aat gaa atg att aat geg ttc 480 -229- 136852-序列表.doc

1 SI 1334884

Glu Met Glu Arg lie Val Gly Ala Val Asn Glu Met lie Asn Ala Phe 145 150 155 160 cga aac gga gaa gtg gat geg gtt tac gtc get tac aac cgt ttt gaa 528 Arg Asn Gly Glu Val Asp Ala Val Tyr Val Ala Tyr Asn Arg Phe Glu 165 170 175 aat aeg atg tea caa aaa cct gtt ate gca cag tta ett ccg tta cct 576 Asn Thr Met Ser Gin Lys Pro Val lie Ala Gin Leu Leu Pro Leu Pro lflO 185 190 aaa eta gat gac gat gaa tta gat aeg aaa ggt tea tgg gat tat att 624 Lys Leu Asp Asp Asp Glu Leu Asp Thr Lys Gly Ser Trp Asp Tyr lie 195 200 205 tat gaa ccg aat cca caa gtt tta ttg gat agt tta ett gtt cgt tat 672 Tyr Glu Pro Asn Pro Gin Val Leu Leu Asp Ser Leu Leu Val Arg Tyr 210 215 220 tta gaa act cag gta tac caa gca gtt gta gat aac eta get tet gaa 720 Leu Glu Thr Gin Val Tyr Gin Ala Val Val Asp Asn Leu Ala Ser Glu 225 230 235 240 caa gcc get cga atg gta geg atg aaa gcc gca aca gat aat geg ggt 768 Gin Ala Ala Arg Met Val Ala Met Lys Ala Ala Thr Asp Asn Ala Gly 245 250 255 aca tta ate gat gaa tta caa tta gtg tat aac aaa get ege caa gca Θ16 Thr Leu lie Asp Glu Leu Gin Leu Val Tyr Asn Lys Ala Arg Gin Ala 260 265 270 age att aca aat gaa tta aac gaa att gtt geg ggt gcc gca gca att 864 Ser lie Thr Asn Glu Leu Asn Glu lie Val Ala Gly Ala Ala Ala He 275 280 285 taa 867 <210> 133 <211> 288 <212> g白甘 <213>胸膜胂炎放線样菌 <400> 133

Met Ala Gly Ala Lys Glu lie Arg Thr Lys lie Ala Ser Val Lys Asn 1 5 10 15

Thr Gin Lys lie Thr Lys Ala Met Glu Met Val Ala Thr Ser Lys Met 20 25 30

Arg Lys Thr Gin Glu Arg Met Ala Ala Ser Arg Pro Tyr Ser Glu Thr 35 40 4 5 lie Arg Lys Val lie Ser His lie Ala Lys Gly Ser lie Gly Tyr Lys 50 55 60

His Pro Phe Leu Thr Glu Arg Asp lie Lys Lys Val Gly Tyr Leu Val 65 70 75 80

Val Ser Thr Asp Arg Gly Leu Cys Gly Gly Leu Asn lie Asn Leu Phe 85 90 95 -230- 136852-序列表.doc ISl 1334884

Asn Ser Ser Leu Ala Ser Gly Gin lie Ser Glu Thr Phe lie Lys lie 50 55 60 tgc ggc gac caa ctt gat caa tat ggg caa aat ttt att cgt gta atg 240

Cys Gly Asp Gin Leu Asp Gin Tyr Gly Gin Asn Phe lie Arg Val Met 65 70 75 80 get gaa aat aaa cgt ctg get gtg ttg cct atg gtt ttt gat act ttc 288

Ala Glu Asn Lys Arg Leu Ala Val Leu Pro Met Val Phe Asp Thr Phe 85 90 95 gta tea tta ega geg gaa cat gaa geg gta aaa gat gta aca att gtt 336

Val Ser Leu Arg Ala Glu His Glu Ala Val Lys Asp Val Thr lie Val 100 105 110 teg gca aac gaa tta agt caa gca caa gaa gat daa ate gca aaa geg 384

Ser Ala Asn Glu Leu Ser Gin Ala Gin Glu Asp Lys lie Ala Lys Ala 115 120 125 atg gaa aaa ege tta ggt caa aaa gtt cgt tta acc aac caa ate gat 432

Met Glu Lys Arg Leu Gly Gin Lys Val Arg Leu Thr Asn Gin lie Asp 130 135 X40 aac age ctg att gca ggc gta att att aaa tac gat gat gtt gtt att 480

Asn Ser Leu lie Ala Gly Val lie He Lys Tyr Asp Asp Val Val lie 145 150 155 160 gat ggt agt age ege ggt cag tta aat ege tta geg tea geg ttg age 528

Asp Gly Ser Ser Arg Gly Gin Leu Asn Arg Leu Ala Ser Ala Leu Ser 165 170 175 ttg taa 534

Leu <210> 135 <211> 177 <212>浪白货 <213=>胸膜胂炎枝綿桴逋 <400> 135

Met Ser Glu Leu Ser Thr Val Ala Arg Pro Tyr Ala Lys Ala Ala Phe IS 10 15

Asp Phe Ala Leu Glu Gin Gly Gin Leu Asp Lys Trp Gin Glu Met Leu 20 25 30

Gin Phe Ser Ala Phe Val Ala Glu Asn Glu Gin Val Ala Glu Tyr lie 35 40 45 "

Asn Ser Ser Leu Ala Ser Gly Gin lie Ser Glu Thr Phe lie Lys lie 50 55 60

Cys Gly Asp Gin Leu Asp Gin Tyr Gly Gin Asn Phe He Arg Val Met 65 70 75 80

Ala Glu Asn Lys Arg Leu Ala Val Leu Pro Met Val Phe Asp Thr Phe 85 90 95

Val Ser Leu Arg Ala Glu His Glu Ala Val Lys Asp Val Thr lie Val 100 105 110 -231 - 136852·序列表.doc i Si 1334884

Ser Ala Asn Glu Leu Ser Gin Ala Gin Glu Asp Lys lie Ala Lys Ala 115 120 X25 Met Glu Lys Arg Leu Gly Gin Lys Val Arg Leu Thr Asn Gin lie Asp 130 135 140 Asn Ser Leu lie Ala Gly Val lie lie Lys Tyr Asp Asp Val Val lie 145 150 155 160 Asp Gly Ser Ser Arg Gly Gin Leu Asn Arg Leu Ala Ser Ala Leu Ser 165 170 175 Leu <210> 136 <211> 321 <212> DHA <213=>胸膜肺.¾放竦徉菌

<220> <223> d)csA <220> <221> CDS <222> (1) . . (318) <400> 136 gca tgg cat gtg caa att atg gac gaa get gag cgt aca aaa aac caa Ala Trp His Val Gin lie Met Asp Glu Ala Glu Arg Thr Lys Asn Gin 15 10 15 atg cag gaa gaa gtc get aat ttc gcc gat cct geg gac ege gcc act Met Gin Glu Glu Val Ala Asn Phe Ala Asp Pro Ala Asp Arg Ala Thr 20 25 30 cag gaa gaa gaa ttc agt ett gaa tta aga aac cgt gac cgt gag cgt Gin Glu Glu Glu Phe Ser Leu Glu Leu Arg Asn Arg Asp Arg Glu Arg 35 40 45 aaa ttg ett aag aag att gag caa aeg tta aat age att gcc gaa gac Lys Leu Leu Lys Lys lie Glu Gin Thr Leu Asn Ser He Ala Glu Asp 50 55 60 gaa tac ggc tat tgc gaa act tgc ggt gtt gaa ate ggt tta cgt cgt Glu Tyr Gly Tyr Cys Glu Thr Cys Gly Val Glu lie Gly Leu Arg Arg 65 70 75 80 tta gaa geg ege ccg acc geg gat atg tgt ate gat tgc aaa aca ett Leu Glu Ala Arg Pro Thr Ala Asp Met Cys He Asp Cys Lys Thr Leu 85 90 95 geg gaa ate cgt gaa aag caa atg ggc tta taa Ala Glu lie Arg Glu Lys Gin Met Gly Leu 100 105 48 96 144 192 240 288 321 <210> 137 <211> 106 <213> 膜胂炎放線样蔺 136852-序列表.doc 232- 1334884 <400> 137

Ala Trp His Val Gin lie Met Asp Glu Ala Glu Arq Thr Lys Asn Gin 1 5 10 ' 15

Met Gin Glu Glu Val Ala Asn Phe Ala Asp Pro Ala Asp Arg Ala Thr 20 25 30

Gin Glu Glu Glu Phe Ser Leu Glu Leu Arg Asn Arg Asp Arg Glu Arg 35 40 45

Lys Leu Leu Lys Lys He Glu Gin Thr Leu Asn Ser lie Ala Glu Asp 50 55 60

Glu Tyr Gly Tyr Cys Glu Thr Cys Gly Val Glu lie Gly Leu Arg Arg 65 7 0 7S 80

Leu Glu Ala Arg Pro Thr Ala Asp Met Cys lie Asp Cys Lys Thr Leu 85 90 95

Ala Glu lie Arg Glu Lys Gin Met Gly Leu 100 105

<210> 138 <211> 33 <212> DNA <213>胸膜胂炎故線桴菌 <220>

<223> dnaK <220> <221> CDS <222> (1) . . (30) <400> 138 get gag ttt gaa gaa gtg aaa gat aat aaa taa Ala Glu Phe Glu Glu Val Lys Asp Asn Lys a 5 io

<210> 139 <211> 10 <212>蛋白货 <213>胸饯胂炎故綿桿菌 <400> 139

Ala Glu Phe Glu Glu Val Lys Asp Asn Lys 1 5 l〇

<210> 140 <211> 453 <212> DNA c213> W棋胂.¾放綿样1¾ <220>

<223> exbB <220> <22l> CDS <222> (1) . - (450) -233 · 136852-序列I表.doc 1334884

<210> 141 <211> 150

<400> 140 atg gaa caa atg ett gaa ett tta caa ggt cat gtt gat tat att att 48 Met Glu Gin Met Leu Glu Leu Leu Gin Gly His Val Asp Tyr lie He 1 5 10 15 tta ggc tta tta eta tta atg agt gtt gtg ttg gta tgg aaa att att 96 Leu Gly Leu Leu Leu Leu Met Ser Val Val Leu Val Trp Lys He He 20 25 30 gaa ege gta ett ttc tac aaa c *a ttg gat gtg acc aaa tat gac aeg 144 Glu Arg Val Leu Phe Tyr Lys Gin Leu Asp Val Thr Lys Tyr Asp Thr 35 40 45 eta caa gat ttg gaa att gat acc act ege aat tta acc acc att tcc 192 Leu Gin Asp Leu Glu lie Asp Thr Thr Arg Asn Leu Thr Thr He Ser 50 55 60 act ate ggt gcc aac gcc cct tat ate ggt tta tta gga acc gta tta 240 Thr lie Gly Ala Asn Ala Pro Tyr He Gly Leu Leu Gly Thr Val Leu 65 70 75 80 ggg ate tta ett acc ttc tat cat tta 999 cat tcc ggc ggt gat att 288 Gly lie Leu Leu Thr Phe Tyr His Leu Gly His Ser Gly Gly Asp lie 85 90 95 gac gcc gca tcc att atg gtt cac ett teg ett gca tta aaa gca acc 336 Asp Ala Ala Ser lie Met Val His Leu Ser Leu Ala Leu Lys Ala Thr 100 105 110 gca gcc ggt ate tta gtc get att ccg gca atg atg ttc tac age ggt 384 Ala Ala Gly lie Leu Val Ala lie Pro Ala Met Met Phe Tyr Ser Gly 115 120 125 ttt aac cgt aaa gtg gat gaa age aaa ett aaa tgg caa geg att caa 432 Phe Asn Arg Lys Val Asp Glu Ser Lys Leu Lys Trp Gin Ala lie Gin 130 135 140 get cgt aaa gcc aat caa taa 453 Ala Arg Lys Ala Asn Gin 145 150 <212> ^ϊ:3νϊ <2 13 >构犋胂炎放絲桿菌 <400> 141

Met Glu Gin Met Leu Glu Leu Leu Gin Gly His Val Asp Tyr lie lie 15 10 15

Leu Gly Leu Leu Leu Leu Met Ser Val Val Leu Val Trp Lys lie lie 20 25 30

Glu Arg Val Leu Phe Tyr Lys Gin Leu Asp Val Thr Lys Tyr Asp Thr 35 40 45

Leu Gin Asp Leu Glu lie Asp Thr Thr Arg Asn Leu Thr Thr lie Ser 50 55 60

Thr lie Gly Ala Asn Ala Pro Tyr lie Gly Leu Leu Gly Thr Val Leu 65 70 75 80 •234· 136852-序列表.doc 1S1 1334884

Gly He Leu Leu Thr Phe Tyr His Leu Gly His Ser Gly Gly Asp He 85 90 95 Asp Ala Ala Ser He Met Val His Leu Ser Leu Ala Leu Lys Ala Thr 100 105 110 Ala Ala Gly lie Leu Val Ala lie Pro Ala Met Met Phe Tyr Ser Gly 115 120 125 --he Asn Arg Lys Val Asp Glu Ser Lys Ltru Lys Trp Gin Ala lie Gin 130 135 140 Ala Arg Lys Ala Asn Gin 145 150 <210> 142 <211> 720 <212> DNA <213>构膜胂灸故線桿菌

<220> <223> fkpA <220> <221> CDS <222> (1)..(717) <400> 142 atg tta aaa aat aaa ctt tct gtt ctt gca ate gta gcc ggt aeg ttc Met Leu Lys Asn Lys Leu Ser Val Leu Ala lie Val Ala Gly Thr Phe 15 10 15 gtt tea get caa act gca ttt gca geg gat caa aaa ttc att gac gat Val Ser Ala Gin Thr Ala Phe Ala Ala Asp Gin Lys Phe lie Asp Asp 20 25 30 tea tea tat gca gtc ggc gta ttg atg ggt aaa aat ate gaa ggc gtc Ser Ser Tyr Ala Val Gly Val Leu Met Gly Lys Asn He Glu Gly Val 35 40 45 gtt gaa tea caa aaa gaa att ttt tct tat aac caa gat aaa ate ttg Val Glu Ser Gin Lys Glu He Phe Ser Tyr Asn Gin Asp Lys lie Leu 50 55 60 geg ggt gtc caa gat acc ate aaa aaa acc ggt aaa tta acc gat gaa Ala Gly Val Gin Asp Thr He Lys Lys Thr Gly Lys Leu Thr Asp Qlu 65 70 75 80 gat eta caa aaa caa tta aaa teg ctt gat act tat ctt gca agt caa Asp Leu Gin Lys Gin Leu Lys Ser Leu Asp Thr Tyr Leu Ala Ser Gin 85 90 95 48 96 144 192 240 288 gaa age aaa att geg geg gag aaa age aaa gca acc gta gaa gcc ggt Glu Ser Lys lie Ala Ala Glu Lys Ser Lys Ala Thr Val Glu Ala Gly 100 105 110 aac aaa ttt cgt acc gac tac gaa aaa caa age ggc gtg aaa aaa acc Asn Lys Phe Arg Thr Asp Tyr Glu Lys Gin Ser Gly Val Lys Lys Thr 115 120 125 get tcc gqt eta ctt tat aaa ntt gaa aaa qcc age acc ggc aaa teg 336 384 432 136852-序列表.doc -235-

l SI 1334884

Gly lie Leu Leu Thr Phe Tyr His Leu Gly His Ser Gly Gly Asp lie 85 90 95 Asp Ala Ala Ser lie Met Val His Leu Ser Leu Ala Leu Lys Ala Thr 100 105 110 Ala Ala Gly He Leu Val Ala He Pro Ala Met Met Phe Tyr Ser Gly 115 120 125 =he Asn Arg Lys Va】Asp Glu Ser Lys LfU Lys Trp Gin Ala lie Gin 130 135 140 Ala Arg Lys Ala Asn Gin 145 150

<210> 142 <211> 720 <212> DNA <213>胸膜胂炎故線样菌 <220> <223> fkpA <220> <221> CDS <222> (1)..(717) <400> 142 atg tta aaa aat aaa ctt tct gtt ctt gca ate gta gcc ggt aeg ttc Met Leu Lys Asn Lys Leu Ser Val Leu Ala lie Val Ala Gly Thr Phe 15 10 15 gtt tea get caa act gca ttt gca geg gat caa aaa ttc act gac gat Val Ser Ala Gin Thr Ala Phe Ala Ala Asp Gin Lys Phe He Asp Asp 20 25 30 tea tea tat gca gtc ggc gta ttg atg ggt aaa aat ate gaa ggc gtc Ser Ser Tyr Ala Val Gly Val Leu Met Gly Lys Asn lie Glu Gly Val 35 40 45 gtt gaa tea caa aaa gaa att ttt tct tat aac caa gat aaa ate ttg Val Glu Ser Gin Lys Glu He Phe Ser Tyr Asn Gin Asp Lys lie Leu 50 55 60 geg ggt gtc caa gat acc ate aaa aaa acc ggt aaa tta acc gat gaa Ala Gly Val Gin Asp Thr lie Lys Lys Thr Gly Lys Leu Thr Asp Glu 65 70 75 80 gat eta caa aaa caa tta aaa teg ctt gat act tat ctt gca agt caa Asp Leu Gin Lys Gin Leu Lys Ser Leu Asp Thr Tyr Leu Ala Ser Gin 85 90 95 48 96 144 192 240 286 gaa age aaa att geg geg gag aaa age aaa gca acc gta gaa gcc ggt Glu Ser Lys He Ala Ala Glu Lys Ser Lys Ala Thr Val Glu Ala Gly 100 105 110 aat aaa ttt cgt acc gac tac gaa aaa caa age ggc gtg aaa aaa acc Asn Lys Phe Arg Thr Asp Tyr Glu Lys Gin Ser Gly Val Lys Lys Thr 115 120 125 get tcc gqt tta ctt tat aaa ntt gaa aaa gcc ggc aeg ggc gaa teg 336 384 432 136852-序列表.doc •236·

ί SI 4801334884

Ala Ser Gly Leu Leu Tyr Lys lie Glu Lys Ala Gly Thr Gly Glu Ser 130 135 140 cct aaa gcg gaa gat acc gtt aaa gtt cac tat aaa ggg aca tta acc Pro Lys Ala Glu Asp Thr Val Lys Val His Tyr Lys Gly Thr Leu Thr 145 150 155 160 gat ggt acg gta ttc gat age tea tac gat ege ggt gag ccg att gaa Asp Gly Thr Val Phe Asp Ser Ser Tyr Asp Arg Gly Glu Pro lie Glu X65 170 175 ttc caa tta aac caa tta att ccg ggt tgg att gaa gcg att cca atg Phe Gin Leu Asn Gin Leu lie Pro Gly Trp lie Glu Ala lie Pro Met 180 185 190 ttg aaa aaa ggc gga aaa atg gaa ate gtc gtt ccg cct gaa ett ggt Leu Lys Lys Gly Gly Lys Met Glu lie Val Val Pro Pro Glu Leu Gly 195 200 205 tac ggc gaa ege caa gca ggt aag att ccg gca agt tea acc tta aaa Tyr Gly Glu Arg Gin Ala Gly Lys lie Pro Ala Ser Ser Thr Leu Lys 210 215 220 ttc gag att gaa ttg tta gat ttc aaa gcg gcc gaa gcg aaa aaa taa Phe Glu lie Glu Leu Leu Asp Phe Lys Ala Ala Glu Ala Lys Lys 225 230 235 528 576 624 672 720

<210> 143 <211> 239 <212>蛋白質 <213>胸膜肺炎放線桿菌 <400> X43

Met Leu Lys Asn Lys Leu Ser Val 1 5

Val Ser Ala Gin Thr Ala Phe Ala 20

Ser Ser Tyr Ala Val Gly Val Leu 35 40

Val Glu Ser Gin Lys Glu lie Phe 50 55

Ala Gly Val Gin Asp Thr lie Lys 65 70

Asp Leu Gin Lys Gin Leu Lys Ser 85

Glu Ser Lys lie Ala Ala Glu Lys 100

Asn Lys Phe Arg Thr Asp Tyr Glu 115 120

Ala Ser Gly Leu Leu Tyr Lys lie 130 135

Pro Lys Ala Glu Asp Thr Val Lvs 145 150 ^

Leu Ala lie Val Ala Gly Thr Phe 10 15

Ala Asp Gin Lys Phe lie Asp Asp 25 30

Met Gly Lys Asn lie Glu Gly Val 45

Ser Tyr Asn Gin Asp Lys lie Leu 60

Lys Thr Gly Lys Leu Thr Asp Glu 75 80

Leu Asp Thr Tyr Leu Ala Ser Gin 90 95

Ser Lys Ala Thr Val Glu Ala Gly 105 110

Lys Gin Ser Gly Val Lys Lys Thr 125

Glu Lys Ala Gly Thr Gly Glu Ser 140

Val His Tyr Lys Gly Thr Leu Thr 155 160 -237 136852-序列表.doc 1334884

Asp Gly Thr Val Phe Asp Ser Ser Tyr Asp Arg Gly Glu Pro lie Glu X65 170 175

Phe Gin Leu Asn Gin Leu lie Pro Gly Trp lie Glu Ala lie Pro Met 180 185 190

Leu Lys Lys Gly Gly Lys Met Glu lie Val Val Pro Pro Glu Leu Gly 195 200 205

Tyr Gly Glu Arg Gin Ala Gly Lys lie Pro Ala Ser Ser Thr Leu Lys 210 215 220

Phe Glu lie Glu Leu Leu Asp Phe Lys Ala Ala Glu Ala Lys Lys 225 230 235 <210> 144 <211> 290 <212> DNA <213>胸膜肺炎放綠桿菌 <220> <223> HI0379 <220> <221> CDS <222> (3)..(287) <400> 144 tg cat age gtg aga ggt ccg ggc ggc ggt tat caa etc ggt aag caa His Ser Val Arg Gly Pro Gly Gly Gly Tyr Gin Leu Gly Lys Gin 15 10 15 cct gaa gag att agt gtg ggg atg att att geg geg gtg aat gaa aat Pro Glu Glu lie Ser Val Gly Met lie lie Ala Ala Val Asn Glu Asn 20 25 30 47 95

etc gac gta acc aaa tgt aaa ggt age ggc aac tgt age aaa aac tet Leu Asp Val Thr Lys Cys Lys Gly Ser Gly Asn Cys Ser Lys Asn Ser 35 40 45 cag tgc tta acc cat cat tta tgg gaa cgt tta gaa gaa caa ate ggt Gin Cys Leu Thr His His Leu Trp Glu Arg Leu Glu Glu Gin lie Gly 50 55 60 gtg ttt tta aat aeg att act tta geg gaa ett gtt gaa gaa cat teg Val Phe Leu Asn Thr lie Thr Leu Ala Glu Leu Val Glu Glu His Ser 65 70 75 gat cac gat tgt gaa aaa gaa cat tgc cac gat cat tea cac aaa cat Asp His Asp Cys Glu Lys Glu His Cys His Asp His Ser His Lys His 80 85 90 95 taa 143 191 239 287 290 <210> 145 <211> 95 <212>蛋白筲 <2 1 3 ：>胸棋胂炎放綿桴茵 <400> 145

His Ser Val Arg Gly Pro Gly Gly Gly Tyr Gin Leu Gly Lys Gin Pro 238 - 136852·序列表.doc 1334884 1 5 10 15

Glu Glu lie Ser Val Gly Met lie He Ala Ala Val Asn Glu Asn Leu 20 25 30

Asp Val Thr Lys Cys Lys Gly Ser Gly Asn Cys Ser Lys Asn Ser Gin 35 40 45

Cys Leu Thr His His Leu Trp Glu Arg Leu Glu Glu Gin lie Gly Val 50 55 60

Phe Leu Asn Thr lie Thr Leu Ala Glu Leu Val Giu Glu His Ser Asp 65 70 75 80

His Asp Cys Glu Lys Glu His Cys His Asp His Ser His Lys His 85 90 95

<210> 146 <211> 273 <212> DNA

<213>胸膜肺炎故竦桿菌 <220>

<223> hupA <220> <221> CDS <222> (1)..(270) <400> 146 atg aac aaa act gag tta ate gat gca ate gca get ggt gca gag tta 48

Met Asn Lys Thr Glu Leu lie Asp Ala lie Ala Ala Gly Ala Glu Leu 15 10 15 age aag aaa gac geg aaa geg gca tta gaa geg act tta aat geg ate 96

Ser Lys Lys Asp Ala Lys Ala kla Leu Glu Ala Thr Leu Asn Ala lie 20 25 30 tet gaa age eta aaa aat ggc gac acc gtt cag tta ate ggc ttc ggt 144

Ser Glu Ser Leu Lys Asn Gly Asp Thr Val Gin Leu lie Gly Phe Gly 35 40 45 act ttt aaa gta aac gag cgt aat gca cgt aeg ggt cgt aac ccg cgt 192

Thr Phe Lys Val Asn Glu Arg Asn Ala Arg Thr Gly Arg Asn Pro Arg 50 55 60 acc ggc gaa gaa ate aaa ate gca gca tet aaa gtg ccg geg ttt gtt 240

Thr Gly Glu Glu lie Lys lie Ala Ala Ser Lys Val Pro Ala Phe Val 65 70 75 80 gca ggt aaa gca tta aaa gat tta gta aaa taa 273

Ala Gly Lys Ala Leu Lys Asp Leu Val Lys 85 9〇 <210> 147 <211> 90 <212>蛋白質 <213> W膜肺.¾枚綿样菌 <400> 147

Met Asn Lys Thr Glu Leu lie Asp Ala lie Ala Ala Gly Ala Glu Leu 239- 136852-序列表.doc

I SI 1334884 IS 10 15

Ser Lys Lys Asp Ala Lys Ala Ala Leu Glu Ala Thr Leu Asn Ala lie 20 25 30

Ser Glu Ser Leu Lys Asn Gly Asp Thr Val Gin Leu lie Gly Phe Gly 35 40 45

Thr Phe Lys Val Asn Glu Arg Asn Ala Arg Thr Gly Arg Asn Pro Arg 50 55 60

Thr Gly Glu Glu lie Lys lie Ala Ala Ser Lys Val Pro Ala Phe Val 65 70 75 80

Ala Gly Lys Ala Leu Lys Asp Leu Val Lys 85 90

<210> 148 <211> 551 <212> DNA

<213>胸模胂炎放綿样(¾ <220>

<223> lpdA <220> <221> CDS <222> (1) . . (549) <400> 148 atg age aaa gaa ate aaa aeg caa gtc gtg gta ett ggt geg ggt cct 48

Met Ser Lys Glu lie Lys Thr Gin Val Val Val Leu Gly Ala Gly Pro 15 10 15 gcc ggt tat tea geg gca ttc cgt tgt gcc gac tta ggc tta gaa aca 96

Ala Gly Tyr Ser Ala Ala Phe Arg Cys Ala Asp Leu Gly Leu Glu Thr 20 25 30 gta att gtc gaa cgt tat tea act ttg ggc ggt gta tgc tta aac gta 144

Val lie Val Glu Arg Tyr Ser Thr Leu Gly Gly Val Cys Leu Asn Val 35 40 45

ggt tgt att ccg tet aaa gca tta tta cac gtt gca aaa gtt ate gaa 192

Gly Cys lie Pro Ser Lys Ala Leu Leu His Val Ala Lys Val lie Glu 50 55 60 gaa gca aaa cac gca gag aaa aac ggt att act ttc ggt gag ccc aac 240

Glu Ala Lys His Ala Glu Lys Asn Gly lie Thr Phe Gly Glu Pro Asn 65 70 75 80 att gat tta gat aaa gtg cgt geg ggt aaa gaa geg gtt gtt tet aaa 288 lie Asp Leu Asp Lys Val Arg Ala Gly Lys Glu Ala Val Val Ser Lys

85 90 9S tta acc ggc ggt tta geg ggt atg get aaa gca cgt aaa gta aca gta 336

Leu Thr Gly Gly Leu Ala Gly Met Ala Lys Ala Arg Lys Val Thr Val

100 105 HO gtg gaa ggt tta geg geg ttt acc gat ccg aat act tta gta get cgt 384

Val Glu Gly Leu Ala Ala Phe Thr Asp Pro Asn Thr Leu Val Ala Arg 115 120 125 -240· 136852-序列表.doc

LSI 1334884 gac cgt gac ggt aat ccg aca acg att aaa ttt gat tat gca att att 432

Asp Arg Asp Gly Asn Pro Thr Thr lie Lys Phe Asp Tyr Ala lie lie 130 135 140 gca gcc ggt tct cgt ccg att cag ctt ccg ttc att cca cac gaa gat 480

Ala Ala Gly Ser Arg Pro lie Gin Leu Pro Phe lie Pro His Glu Asp 145 150 155 160 ccg cgt gtg tgg gat tct acg gat gca ctt aaa tta aaa gaa gta ccc 528

Pro Arg Val Trp Asp Ser Thr Asp Ala Leu Lys Leu Lys Glu Val Pro 165 170 175 gaa aaa att act cat tat ggg cc 551

Glu Lys lie Thr His Tyr Gly 180 <210> 149 <211> 183 <212>蛋白筲 <213>胸膜胂炎故線桿鹐

<400> 149

Met Ser Lys Glu lie Lys Thr Gin Val Val Val Leu Gly Ala Gly Pro 15 10 15

Ala Gly Tyr Ser Ala Ala Phe Arg Cys Ala Asp Leu Gly Leu Glu Thr 20 25 30

Val lie Val Glu Arg Tyr Ser Thr Leu Gly Gly Val Cys Leu Asn Val 35 40 45

Gly Cys lie Pro Ser Lys Ala Leu Leu His Val Ala Lys Val lie Glu 50 55 60

Glu Ala Lys His Ala Glu Lys Asn Gly lie Thr Phe Gly Glu Pro Asn 65 70 75 80 lie Asp Leu Asp Lys Val Arg Ala Gly Lys Glu Ala Val Val Ser Lys 85 90 95

Leu Thr Gly Gly Leu Ala Gly Met Ala Lys Ala Arg Lys Val Thr Val 100 105 110

Val Glu Gly Leu Ala Ala Phe Thr Asp Pro Asn Thr Leu Val Ala Arg 115 120 125

Asp Arg Asp Gly Asn Pro Thr Thr lie Lys Phe Asp Tyr Ala lie lie 130 135 140

Ala Ala Gly Ser Arg Pro lie Gin Leu Pro Phe lie Pro His Glu Asp 145 150 155 160

Pro Arg Val Trp Asp Ser Thr Asp Ala Leu Lys Leu Lys Glu Val Pro 165 170 175

Glu Lys lie Thr His Tyr Gly 180

<210> 150 <211> 1095 <212> DNA -241 - 136852·序列表.doc

I 1334884

<213>胸膜肺炎放線桴菌 <220> < 2 2 3 > Omp 5-2 <220> <221> CDS <222> (X) .. (1092) <400> 150 atg aaa aaa tea tta gtt get tta aca gta tta teg get gca geg gta Met Lys Lys Ser Leu Val Ala Leu Thr Val Leu Ser Ala Ala Ala Val 15 10 15 get caa gca geg cca caa caa aat act ttc tac gca ggt geg aaa gca Ala Gin Ala Ala Pro Gin Gin Asn Thr Phe Tyr Ala Gly Ala Lys Ala 20 25 30 tgg geg tea ttc cat gat ggt ate gaa caa tta gat tea get aaa Gly Trp Ala Ser Phe His Asp Gly lie Glu Gin Leu Asp Ser Ala Lys 35 40 45 aac aca gat ege ggt aca aaa tac ggt ate aac cgt aat tea gta act Asn Thr Asp Arg Gly Thr Lys Tyr Gly He Asn Arg Asn Ser Val Thr 50 55 60 tac ggc gta ttc ggc ggt tac caa att tta aac caa gac aaa tta ggt Tyr Gly Val Phe Gly Gly Tyr Gin lie Leu Asn Gin Asp Lys Leu Gly 65 70 75 80 tta geg get gaa tta ggt tat gac tat ttc ggt cgt gtg ege ggt tet Leu Ala Ala Glu Leu Gly Tyr Asp Tyr Phe Gly Arg Val Arg Gly Ser 85 90 95 gaa aaa cca aac ggt aaa geg gac aag aaa act ttc cgt cac get gca Glu Lys Pro Asn Gly Lys Ala Asp Lys Lys Thr Phe Arg His Ala Ala 100 105 110 cac ggt geg aca ate gca tta aaa cct age tac gaa gta tta cct gac His Gly Ala Thr He Ala Leu Lys Pro Ser Tyr Glu Val Leu Pro Asp 115 120 125 tta gac gtt tac ggt aaa gta ggt ate gca tta gta aac aat aca tat Leu Asp Val Tyr Gly Lys Val Gly lie Ala Leu Val Asn Asn Thr Tyr 130 135 140 aaa aca ttc aat gca gca caa gag aaa gtg aaa act cgt cgt ttc caa Lys Thr Phe Asn Ala Ala Gin Glu Lys Val Lys Thr Arg Arg Phe Gin 145 150 155 160 agt tet tta att tta ggt geg ggt gtt gag tac gca att ett cct gaa Ser Ser Leu He Leu Gly Ala Gly Val Glu Tyr Ala He Leu Pro Glu 165 170 175 tta geg gca cgt gtt gaa tac caa tgg tea aac aac gca ggt aaa gca Leu Ala Ala Arg Val Glu Tyr Gin Trp Leu Asn Asn Ala Gly Lys Ala 180 185 190 age tac tet act tta aat cgt atg ggt gca act gac tac cgt teg gat Ser Tyr Ser Thr Leu Asn Arg Met Gly Ala Thr Asp Tyr Arg Ser Asp 195 2〇〇 205 136852-序歹!J表.doc 48 96 144 192 240 288 336 384 432 4Θ0 528 576 •242· t ] 624 6721334884

ate agt tee gta tet gca ggt tta age tac cgt ttc ggt caa ggt geg lie Ser Ser Val Ser Ala Gly Leu Ser Tyr Arg Phe Gly Gin Gly Ala 210 215 220 gca ccg gtt gca get ccg gca gtt gaa act aaa aac ttc gca ttc age Ala Pro Val Ala Ala Pro Ala Val Glu Thr Lys Asn Phe Ala Phe Ser 225 230 235 240 tet gac gta tta ttc gca ttc ggt aaa tea aac tta aaa ccg get geg Ser Asp Val Leu Phe Ala Phe Cly Lys Ser Asn Leu Lys Pro Ala Ala 245 250 255 gca aca gca tta gat gca atg caa acc gaa ate aat aac gca ggt tta Ala Thr Ala Leu Asp Ala Met Gin Thr Glu lie Asn Asn Ala Gly Leu 260 265 270 tea aat get geg ate caa gta aac ggt tac aeg gac cgt ate ggt aaa Ser Asn Ala Ala lie Gin Val Asn Gly Tyr Thr Asp Arg lie Gly Lys 275 280 285 gaa get tea aac tta aaa ett tea caa cgt cgt geg gaa aca gta get Glu Ala Ser Asn Leu Lys Leu Ser Gin Arg Arg Ala Glu Thr Val Ala 290 295 300 aac tac ate gtt tet aaa ggt get ccg gca get aac gta act gca gta Asn Tyr lie Val Ser Lys Gly Ala Pro Ala Ala Asn Val Thr Ala Val 305 310 315 320 ggt tac ggt gaa gca aac cct gta acc ggc gca aca tgt gac aaa gtt Gly Tyr Gly Glu Ala Asn Pro Val Thr Gly Ala Thr Cys Asp Lys Val 325 330 335 aaa ggt cgt aaa gca tta ate get tgc tta gca ccg gat cgt cgt gtt Lys Gly Arg Lys Ala Leu lie Ala Cys Leu Ala Pro Asp Arg Arg Val 340 345 350 gaa gtt caa gtt caa ggt act aaa gaa gta act atg taa Glu Val Gin Val Gin Gly Thr Lys Glu Val Thr Met 355 360 720 768 816 864 912 960 1006 1056 1095

<210> 151 <211> 364 <212>蛋白質 <2：L3>胸膜肺炎故線桿菌 <400> 151

Met Lys Lys Ser Leu Val Ala Leu Thr Val Leu Ser Ala Ala Ala Val 15 10 15

Ala Gin Ala Ala Pro Gin Gin Asn Thr Phe Tyr Ala Gly Ala Lys Ala 20 25 30

Gly Trp Ala Ser Phe His Asp Gly lie Glu Gin Leu Asp Ser Ala Lys 35 40 45

Asn Thr Asp Arg Gly Thr Lys Tyr Gly He Asn Arg Asn Ser Val Thr 50 55 60

Tyr Gly Val Phe Gly Gly Tyr Gin lie Leu Asn Gin Asp Lys Leu Gly 65 70 75 80

Leu Ala Ala Glu Leu Gly Tyr Asp Tyr Phe Gly Arg Val Arg Gly Ser 243- 136852-序列表.doc i 1334884 85 90 95

Glu Lys Pro Asn Gly Lys Ala Asp Lys Lys Thr Phe Arg His Ala Ala 100 105 110

His Gly Ala Thr lie Ala Leu Lys Pro Ser Tyr Glu Val Leu Pro Asp 115 120 125

Leu Asp Val Tyr Gly Lys Val Gly lie Ala Leu Val Asn Asn Thr Tyr 130 135 140

Lys Thr Phe Asn Ala Ala Gin Glu Lys Val Lys Thr Arg Arg Phe Gin 145 ISO 155 160

Ser Ser Leu lie Leu Gly Ala Gly Val Glu Tyr Ala lie Leu Pro Glu 165 170 X75

Leu Ala Ala Arg Val Glu Tyr Gin Trp Leu Asn Asn Ala Gly Lys Ala 180 185 190

Ser Tyr Ser Thr Leu Asn Arg Met Gly Ala Thr Asp Tyr Arg Ser Asp 195 200 205 lie Ser Ser Val Ser Ala Gly Leu Ser Tyr Arg Phe Gly Gin Gly Ala 210 2X5 220

Ala Pro Val Ala Ala Pro Ala Val Glu Thr Lys Asn Phe Ala Phe Ser 225 230 235 240

Ser Asp Val Leu Phe Ala Phe Gly Lys Ser Asn Leu Lys Pro Ala Ala 245 250 255

Ala Thr Ala Leu Asp Ala Met Gin Thr Clu lie Asn Asn Ala Gly Leu 260 265 270

Ser Asn Ala Ala He Gin Val Asn Gly Tyr Thr Asp Arg lie Gly Lys 275 280 285

Glu Ala Ser Asn Leu Lys Leu Ser Gin Arg Arg Ala Glu Thr Val Ala 290 295 300

Asn Tyr lie Val Ser Lys Gly Ala Pro Ala Ala Asn Val Thr Ala Val 305 310 315 320

Gly Tyr Gly Glu Ala Asn Pro Val Thr Gly Ala Thr Cys Asp Lys Val 325 330 335

Lys Gly Arg bys Ala Leu lie Ala Cys Leu Ala Pro Asp Arg Arg Val 340 345 350

Glu Val Gin Val Gin Gly Thr Lys Glu Val Thr Met 355 360

<210> 152 <211> 1110 <212> DNA <213>胸膜胂炎放绵桿菌 <220> <223> Omp5 <220> • 244- 136852-序列表.doc 1334884 <221> CDS <222> (1)..(1107)

<400> 152 atg aaa aaa tea tta gtt get tta gca gta tta teg get gca gca gta 48 Met Lys Lys Ser Leu Val Ala Leu Ala Val Leu Ser Ala Ala Ala Val 1 5 10 15 get caa gca get cca caa caa aat act ttc tac gca ggt geg aaa gtt 96 Ala Gin Ala Ala Pro Gin Gin Asn Thr Phe Τ'/T Ala Gly Ala Lys Val 20 25 30 ggt caa tea tea ttt cac cac ggt gtt aac caa tta aaa tet ggt cac 144 Gly Gin Ser Ser Phe His His Gly Val Asn Gin Leu Lys Ser Gly His 35 40 45 gat gat cgt tat aat gat aaa aca cgt aag tat ggt ate aac cgt aac 192 Asp Asp Arg Tyr Asn Asp Lys Thr Arg Lys Tyr Gly lie Asn Arg Asn 50 55 60 tet gta act tac ggt gta ttc ggc ggt tac caa ate tta aac caa aat 240 Ser Val Thr Tyr Gly Val Phe Gly Gly Tyr Gin lie Leu Asn Gin Asn 65 70 75 80 aac ttc ggt tta gca get gaa tta ggc tat gac tac tac ggt ege gta 288 Asn Phe Gly Leu Ala Ala Glu Leu Gly Tyr Asp Tyr Tyr Gly Arg Val 85 90 95 cgt ggt aac gta gat gaa ttc cgt aca gtt aaa cac tet get cac ggt 336 Arg Gly Asn Val Asp Glu Phe Arg Thr Val Lys His Ser Ala His Gly 100 105 110 tta aac tta geg tta aaa cca age tac gaa gta tta cct gac tta gac 384 Leu Asn Leu Ala Leu Lys Pro Ser Tyr Glu Val Leu Pro Asp Leu Asp 115 120 125 gtt tac ggt aaa gta ggt att geg gtt gtt cgt aat gac tat aaa aaa 432 Val Tyr Gly Lys Val Gly He Ala Val Val Arg Asn Asp Tyr Lys Lys 130 135 140 tat ggt geg gaa aac act aac gaa tea aca aca aaa ttc cac aaa tta 480 Tyr Gly Ala Glu Asn Thr Asn Glu Ser Thr Thr Lys Phe His Lys Leu 145 150 155 160 aaa gca tea act att tta ggt gca ggt gtt gag tac gca att ett cct 528 Lys Ala Ser Thr lie Leu Gly Ala Gly Val Glu Tyr Ala lie Leu Pro 165 170 175 gaa tta geg gca cgt gtt gaa tac caa tac tta aac aaa geg ggt aac 576 Glu Leu Ala Ala Arg Val Glu Tyr Gin Tyr Leu Asn Lys Ala Gly Asn 180 185 190 tta aat aaa gca eta gtt cgt tea ggc aca caa gat gtg gac ttc caa 624 Leu Asn Lys Ala Leu Val Arg Ser Gly Thr Gin Asp Val Asp Phe Gin 195 200 205 tat get cct gat ate cac tet gta aca gca ggt tta tea tac cgt ttc 672 Tyr Ala Pro Asp lie His Ser Val Thr Ala Gly Leu Ser Tyr Arg Phe 210 215 220 ggt caa ggc get gta gca cca gtt gtt gag cca gaa gtt gta act aaa 720 Gly Gin Gly Ala val Ala Pro Val Val Glu Pro Glu Val Val Thr Lys 225 230 235 240 136852-序列表.doc -245- 7681334884 aac ttc gca ttc age tea gac gtt tta ttt gat ttc ggt aaa tea age Asn Phe Ala Phe Ser Ser Asp Val Leu Phe Asp Phe Gly Lys Ser Ser 245 250 255 tta aaa cca gca gca gca aca get tta gac gca get aac act gaa ate Leu Lys Pro Ala Ala Ala Thr Ala Leu Asp Ala Ala Asn Thr Glu He 260 265 270 816 get aac tta ggt tta gca act cca get ate caa gtt aac ggt tat aca Ala Asn Leu Gly Leu Ala Thr Pro Ala lie Gin Val Asn Gly Tyr Thr 275 280 285 864 gac cgt ate ggt aaa gaa get tea aac tta aaa ett tea caa ege cgt Asp Arg lie Gly Lys Glu Ala Ser Asn Leu Lys Leu Ser Gin Arg Arg 290 295 300 912 gca gaa act gta get aac tac tta gtt tet aaa ggt caa aac cct gca Ala Glu Thr Val Ala Asn Tyr Leu Val Ser Lys Gly Gin Asn Pro Ala 305 310 315 320 960 aac gta act gca gta ggt tac ggt gaa gca aac cca gta acc ggc gca Asn Val Thr Ala Val Gly Tyr Gly Glu Ala Asn Pro Val Thr Gly Ala 325 330 335 aca tgt gat gca gtt aaa ggt cgt aaa gca tta ate get tgc tta gca Thr Cys Asp Ala Val Lys Gly Arg Lys Ala Leu lie Ala Cys Leu Ala 340 345 350 1008 1056 ccg gat cgt cgt gtt gaa gtt caa gta caa ggt get aaa aac gta get Pro Asp Arg Arg Val Glu Val Gin Val Gin Gly Ala Lys Asn Val Ala 355 360 365 1104 atg taa Met 1110

桿L 線Sr 放se 質^;ys 3 9 3 L· 5 6·'^5 1 3蛋胸 1 s Λ Λ Λ Λ >Ly 0 12 3 0 1111 otf 2 2 2 2 4 e < < < < < H

Ala Gly Asp

Gin Gin Asp 50

Ala Ser 35 Arg

Ala 20 Ser Tyr

Pro Phe Asn

Gin His Asp

Ala Leu Ala Val Leu Ser Ala Ala Ala Val 10 15 Gin Asn Thr Phe Tyr Ala Gly Ala Lys Val 25 30 His Gly Val Asn Gin Leu Lys Ser Gly His 40 45 Lys Thr Arg Lys Tyr Gly lie Asn Arg Asn 55 60

Ser Val Thr Tyr Gly Val Phe Gly Gly Tyr Gin lie Leu Asn Gin Asn 65 70 75 80

Asn Phe Gly Leu Ala Ala Glu Leu Gly Tyr Asp Tyr Tyr Gly Arg Val 85 90 95

Arg Gly Asn Val Asp Glu Phe Arg Thr Val Lys His Ser Ala His Gly 100 105 110 -246- 136852-序列表.doc 1334884

Leu Asn Leu Ala Leu Lys Pro Ser Tyr Glu Val Leu Pro Asp Leu Asp 115 120 125

Val Tyr Gly Lys Val Gly lie Ala Val Val Arg Asn Asp Tyr Lys Lys 130 135 140

Tyr Gly Ala Glu Asn Thr Asn Glu Ser Thr Thr Lys Phe His Lys Leu 145 150 155 160

Lys Ala Ser Thr lie Leu Gly Ala Gly Val Glu Tyr Ala lie Leu Pro 165 170 175

Glu Leu Ala Ala Arg Val Glu Tyr Gin Tyr Leu Asn Lys Ala Gly Asn 180 185 190

Leu Asn Lys Ala Leu Val Arg Ser Gly Thr Gin Asp Val Asp Phe Gin 195 200 205

Tyr Ala Pro Asp lie His Ser Val Thr Ala Gly Leu Ser Tyr Arg Phe 210 215 220

Gly Gin Gly Ala Val Ala Pro Val Val Glu Pro Glu Val Val Thr Lys 225 230 235 240

Asn Phe Ala Phe Ser Ser Asp Val Leu Phe Asp Phe Gly Lys Ser Ser 245 250 255

Leu Lys Pro Ala Ala Ala Thr Ala Leu Asp Ala Ala Asn Thr Glu lie 260 265 270

Ala Asn Leu Gly Leu Ala Thr Pro Ala lie Gin Val Asn Gly Tyr Thr 275 280 285

Asp Arg lie Gly Lys Glu Ala Ser Asn Leu Lys Leu Ser Gin Arg Arg 290 295 300

Ala Glu Thr Val Ala Asn Tyr Leu Val Ser Lys Gly Gin Asn Pro Ala 305 310 315 320

Asn Val Thr Ala Val Gly Tyr Gly Glu Ala Asn Pro Val Thr Gly Ala 325 330 335

Thr Cys Asp Ala Val Lys Gly Arg Lys Ala Leu lie Ala Cys Leu Ala 340 345 350

Pro Asp Arg Arg Val Glu Val Gin Val Gin Gly Ala Lys Asn Val Ala 355 360 365

Met

<210> 154 <211> 1076 <212> DNA <213>胸躲胂炎故綵桿铕 <220> <223> pnp new <220> <221> CDS <222> (1) . . (1074) -247- 136852-序列表.doc IS1 1334884 <400> 154 aat att aaa gaa ttc gta aaa gaa gcg ggt aaa ccg cgt tgg gat tgg 48 Asn He 1-ys Glu Phe Vai Lys Glu Ala Gly Lys Pro Arg Trp Asp Trp 15 10 15 gtt gcg ccg gaa ccg aat acc gca tta ate aac caa gtt aaa gcg tta 96 Val Ala Pro Glu Pro Asn Thr Ala Leu lie Asn Gin Val Lys Ala Leu 20 25 30 geg gaa geg cgt ate ggc gat geg tat cgt att aca gaa aaa caa geg 144 Ala Glu Ala Arg He Gly Asp Ala Tyr Arg lie Thr Glu Lys Gin Ala 35 40 45 cgt tac gaa caa ate gat gca att aaa geg gat gtt ate gca caa tta 192 Arg Tyr Glu Gin lie Asp Ala lie Lys Ala Asp Val lie Ala Gin Leu 50 55 60 acc gca caa gac gaa acc gtt tet gaa ggt geg att att gat att att 240 Thr Ala Gin Asp Glu Thr Val Ser Glu Gly Ala lie He Asp He lie 65 70 75 80

acc gca tta gaa agt tet att gtt ege ggt cgt att att gee ggc gaa 288 Thr Ala Leu Glu Ser Ser lie Val Arg Gly Arg lie lie Ala Gly Glu 85 90 95 ccg cgt att gac ggt cgt aeg gta gat aeg gtt cgt gca tta gac att 336 Pro Arg He Asp Gly Arg Thr Val Asp Thr Val Arg Ala Leu Asp lie 100 105 110 tgc acc ggc gta tta cct cgt aeg cac ggt tet gca ate ttt act ege 384 Cys Thr Gly Val Leu Pro Arg Thr His Gly Ser Ala He Phe Thr Arg 115 120 125 ggt gaa aca caa gca tta geg gtt gca acc tta ggt act gag ege gat 432 Gly Glu Thr Gin Ala Leu Ala Val Ala Thr Leu Gly Thr Glu Arg Asp 130 135 140 gca caa att gtt gac gaa tta acc ggc gag aaa tea gac cgt ttc tta 480 Ala Gin lie Val Asp Glu Leu Thr Gly Glu Lys Ser Asp Arg Phe Leu 145 150 155 160 ttc cac tat aac ttc cct ccg tac tet gtc ggt gaa acc ggt cgt ate 528 Phe His Tyr Asn Phe Pro Pro Tyr Ser Val Gly Glu Thr Gly Arg lie 165 170 175

ggt teg ccg aaa cgt cgt gaa ate ggc cac ggt cgt tta geg aaa ege 576 Gly Ser Pro Lys Arg Arg Glu lie Gly His Gly Arg Leu Ala Lys Arg 180 185 190 ggt gta tta geg gta atg ccg act get gaa gaa ttc ccg tat gta gtg 624 Gly Val Leu Ala Val Met Pro Thr Ala Glu Glu Phe Pro Tyr Val Val 195 200 205 ege gta gta tet gaa att acc gaa tea aac ggt tet tet tea atg get 672 Arg Val Val Ser Glu He Thr Glu Ser Asn Gly Ser Ser Ser Met Ala 210 215 220 tee gta tgc ggc gca tet tta geg tta atg gac gca ggc gta ccg att 720 Ser Val Cys Gly Ala Ser Leu Ala Leu Met Asp Ala Gly Val Pro lie 225 230 235 240 aaa geg geg gtt geg ggt ate gca atg ggc tta gtg aaa gaa gaa gaa 768 Lys Ala Ala Val Ala Gly lie Ala Met Gly Leu Val Lys Glu Glu Glu -248- 136852-序列表.doc

i SI 1334884 gca gca ttc tat cga aaa gaa tta aat ttc aaa ata gta caa gaa tea 2467

Ala Ala Phe Tyr Arg Lys Glu Leu Asn Phe Lvs lie Val Gin Glu Ser 295 ^ * 300 " 305 eta aaa cat aca ate aat act gtt ggt atg ata ate tgg gtc ggc att 2515

Leu Lys His Thr lie Asn Thr Val Glv Met Tie lie Trp Val Gly lie 310 315 * 320 ggc gca aca atg att ata ggt att tat aat eta atg ggt ggg gac cga 2563

Phe lie Ala Asn Leu Phe Ala Ser Leu Asp Ala Ser Pro He Tyr Thr 345 350 355 ate att att atg atg gtt att tta tta ata ett ggt atg ttc tta gat 2659 lie lie lie Met Met Val lie Leu Leu lie Leu Gly Met Phe Leu Asp 360 365 370 tgg att ggt gtt gcc atg ttg act ttc etc aag aca agt aaa geg aca 2707

Ser Phe His Ser Thr Asn Val His Arg Gly Thr Phe Val Gly Arg Gly 405 410 415 420 act ttt tagtaaatet tgcgcgatac gaataaacgc attgatggea tttgctccgt 2859 Thr Phe tttgtggatc gactgccgca tgageagatt tgccaaaaaa ttcaattaca tacttcccaa 2919 tcccttttct ttcgttaacg tttccactta gattgcccag aageegatet gtctgaatgg 2979 gaacaagtgt tataccaaga agcgaatcca acaggtgaag tggtgatcgg tatggtgggt 3039 aaatacactg aattaccgga tgcctacaaa tcggttaatg aagccttgaa acacgcaggc 3099

ttaaaaaacc gtcttagcgt gcaaatcaaa tatattgatt cacaagatgt ggaaaccaaa 3159 ggcacagaag tgttagaagg cgt 3182 <210> 73 <211> 422 <212>蛋白質 <213>敗血性巴斯德•桿菌 <400> 73

Met Pro His His Cys Cys Ser Phe Ser Thr Thr Tyr lie Pro Ser His 15 10 15

Leu Tyr Lys He Ser Gly Gly He He Met He Ser Ala Phe Gly lie 20 25 30

Gly He Gly Thr Leu lie lie Phe Leu Met Met He Ser Leu Leu Phe 35 40 45 •249· 136852-序列表.doc

I SI 1334884 130 135 140

Ala Gin lie Val Asp Glu Leu Thr Gly Glu Lys Ser Asp Arg Phe Leu 145 150 155 160

Phe His Tyr Asn Phe Pro Pro Tyr Ser Val Gly Glu Thr Gly Arg lie 165 170 175

Gly Ser Pro Lys Arg Arg Glu lie Gly His Gly Arg Leu Ala Lys Arg 180 185 190

Gly Val Leu Ala Val Met Pro Thr Ala Glu Glu Phe Pro Tyr Val Val 195 200 205

Arg Val Val Ser Glu lie Thr Glu Ser Asn Gly Ser Ser Ser Met Ala 210 215 220

Ser Val Cys Gly Ala Ser Leu Ala Leu Met Asp Ala Gly Val Pro lie 225 230 235 240

Lys Ala Ala Val Ala Gly lie Ala Met Gly Leu Val Lys Glu Glu Glu 245 250 255

Lys Phe Val Val Leu Ser Asp lie Leu Gly Asp Glu Asp His Leu Gly 260 265 270

Asp Met Asp Phe Lys Val Ala Gly Thr Arg Glu Gly Val Thr Ala Leu 275 280 285

Gin Met Asp He Lys He Glu Gly lie Thr Pro Glu lie Met Gin lie 290 295 300

Ala Leu Asn Gin Ala Lys Gly Ala Arg Met His lie Leu Ser Val Met 305 310 315 320

Glu Gin Ala lie Pro Ala Pro Arg Ala Asp lie Ser Asp Phe Ala Pro 325 330 335

Arg lie His Thr Met Lys lie Asp Pro Lys Lys lie Lys Asp Val lie 340 345 350

Gly Lys Gly Gly Ala Val 355

<210> 156 <211> 1055 <212> DNA <213>胸膜胂炎放埤桿菌 <220>

<223> potD <220> <221> CDS <222> (1) . . (1053) <400> 156 atg aaa aaa tta gcg ggt tta ttt gca gca ggt tta gcg aca gtt gca 48

Met Lys Lys Leu Ala Gly Leu Phe Ala Ala Gly Leu Ala Thr Val Ala 15 10 15 tta aca gcg tgt aat gaa gaa aag cca aaa gcg get gaa aca gcg get 96 250- 136852·序歹暖.doc is] 1334884

Leu Thr Ala Cys Asn Glu Glu Lys Pro Lys Ala Ala Glu Ala Ala Ala 20 25 30 caa ccg gca gca gcg gga aca gtt cac ctt tat act tgg act gaa tat 144

Gin Pro Ala Ala Ala Gly Thr Val His Leu Tyr Thr Trp Thr Glu Tyr 35 40 45 gtg cct gaa ggc ttg tta gat gaa ttt aca aag caa acc ggt ate aaa 192

Val Pro Glu Gly Leu Leu Asp Glu Phe Thr Lys Gin Thr Gly lie Lys SO 55 60 gta gag gtt tea age ctt gaa tet aac gaa acc atg tat gcg aaa tta 240

Val Glu Val Ser Ser Leu Glu Ser Asn Glu Thr Met Tyr Ala Lys Leu 65 70 75 80 aaa tta caa ggt aaa gac ggc ggt tac gat gtt ate gca cct tet aac 288

Lys Leu Gin Gly Lys Asp Gly Gly Tyr Asp Val lie Ala Pro Ser Asn 85 90 95 tac ttc gtt tea aaa atg gcg aaa gaa ggt atg tta gcg gaa tta gat 336

Tyr Phe Val Ser Lys Met Ala Lys Glu Gly Met Leu Ala Glu Leu Asp 100 105 110

cac gca aaa ctt cct gta ate aaa gag tta aac caa gat tgg tta aac 384

His Ala Lys Leu Pro Val lie Lys Glu Leu Asn Gin Asp Trp Leu Asn 115 120 125 aaa cct tat gac caa ggt aac aaa tac tet tta ccg caa tta tta ggt 432

Lys Pro Tyr Asp Gin Gly Asn Lys Tyr Ser Leu Pro Gin Leu Leu Gly 130 135 140 gca ccg ggt ate gca ttt aac tea aat gac tat aag ggc gat gcg ttc 480

Ala Pro Gly lie Ala Phe Asn Ser Asn Asp Tyr Lys Gly Asp Ala Phe 145 150 155 160 act tet tgg ggt gat tta tgg aaa cct gag ttt gcg aat aaa gta caa 528

Thr Ser Trp Gly Asp Leu Trp Lys Pro Glu Phe Ala Asn Lys Val Gin 165 170 175 tta tta gat gac gca cgt gaa gta ttt aac att gcg tta tta aaa tta 576

Leu Leu Asp Asp Ala Arg Glu Val Phe Asn lie Ala Leu Leu Lys Leu 180 185 190 ggt aaa aac cct aat aca acc aat ccg gaa gag att aaa gcg get tac 624

Gly Lys Asn Pro Asn Thr Thr Asn Pro Glu Glu lie Lys Ala Ala Tyr 195 200 205 gaa gag tta aga aaa tta cgt cca aac gta ctt tet ttc act tea gac 672

Glu Glu Leu Arg Lys Leu Arg Pro Asn Val. Leu Ser Phe Thr Ser Asp 210 215 220 aac cca gcg aac tea ttt ate gca ggt gaa gta tet gta ggt caa tta 720

Asn Pro Ala Asn Ser Phe lie Ala Gly Glu Val Ser Val Gly Gin Leu 225 230 235 240 tgg aac ggt tet gta cgt att gcg aaa aaa gaa caa gcg ccg gta aac 768

Trp Asn Gly Ser Val Arg lie Ala Lys Lys Glu Gin Ala Pro Val Asn 245 250 255 atg gtg etc cca aaa gaa ggt cct gta ctt tgg gtt gat aeg tta gcc 816

Met Val Phe Pro Lys Glu Gly Pro Val Leu Trp Val Asp Thr Leu Ala -251 - 136852-序列表.doc ΐ si 8641334884 260 265 270 att ccg gcg aat gcg aaa aac aaa gaa aat gcg cat aag tta ate aac lie Pro Ala Asn Ala Lys Asn Lys Glu Asn Ala His Lys Leu lie Asn 275 280 285 tac tta tta age gca ccg gtt gcg gaa aaa tta aeg tta gaa ate ggt Tyr Leu Leu Ser Ala Pro Val Ala Glu Lys Leu Thr Leu Glu lie Gly 290 295 300 tat ccg act tea aac gta gaa gcg tta aaa aca tta cca aaa gag att Tyr Pro Thr Ser Asn Val Glu Ala Leu Lys Thr Leu Pro Lys Glu lie 305 310 315 320 acc gaa gat ccg gca ate tat ccg aca get gat gtg tta aaa gcg gca Thr Glu Asp Pro Ala lie Tyr Pro Thr Ala Asp Val Leu Lys Ala Ala 325 330 335 caa tgg caa gac gat gta ggt aat gca ate gaa ett tac gaa aaa ta Gin Trp Gin Asp Asp Val Gly Asn Ala lie Glu Leu Tyr Glu Lys 340 345 350 912 960 1008 1055

<210> 157 <211> 351 <212>安白筲 <213>胸膜胂炎故線桿茁

<400> 157 Met Lys Lys Leu Ala Gly Leu Phe Ala Ala Gly Leu Ala Thr Val Ala 15 10 15 Leu Thr Ala Cys Asn Glu Glu Lys Pro Lys Ala Ala Glu Ala Ala Ala 20 25 30 Gin Pro Ala Ala Ala Gly Thr Val His Leu Tyr Thr Trp Thr Glu Tyr 35 40 45 Val Pro Glu Gly Leu Leu Asp Glu Phe Thr Lys Gin Thr Gly lie Lys 50 55 60 Val Glu Val Ser Ser Leu Glu Ser Asn Glu Thr Met Tyr Ala Lys Leu 65 70 75 80 Lys Leu Gin Gly Lys Asp Gly Gly Tyr Asp Val lie Ala Pro Ser Asn 85 90 95 Tyr Phe Val Ser Lys Met Ala Lys Glu Gly Met Leu Ala Glu Leu Asp X00 105 110 His Ala Lys Leu Pro Val lie Lys Glu Leu Asn Gin Asp Trp Leu Asn 115 120 125 Lys Pro Tyr Asp Gin Gly Asn Lys Tyr Ser Leu Pro Gin Leu Leu Gly 130 135 140 Ala Pro Gly lie Ala Phe Asn Ser Asn Asp Tyr Lys Gly Asp Ala Phe 145 150 155 160 Thr Ser Trp Gly Asp Leu Trp Lys Pro Glu Phe Ala Asn Lys Val Gin 165 170 175 Leu Leu Asp Asp Ala Arg Glu Val Phe Asn He Ala Leu Leu Lys Leu 136852·序列表.doc -252 - 1334884 180 185 190

Gly Lys Asn Pro Asn Thr Thr Asn Pro Glu Glu lie Lys Ala Ala Tyr 195 200 205

Glu Glu Leu Arg Lys Leu Arg Pro Asn Val Leu Ser Phe Thr Ser Asp 210 215 220

Asn Pro Ala Asn Ser Phe He Ala Gly Glu Val Ser Val Gly Gin Leu 225 230 225 240

Trp Asn Gly Ser Val Arg lie Ala Lys Lys Glu Gin Ala Pro Val Asn 245 250 255

Met Val Phe Pro Lys Glu Gly Pro Val Leu Trp Val Asp Thr Leu Ala 260 265 270 lie Pro Ala Asn Ala Lys Asn Lys Glu Asn Ala His Lys Leu lie Asn 275 280 285

Tyr Leu Leu Ser Ala Pro Val Ala Glu Lys Leu Thr Leu Glu lie Gly 290 295 300

Tyr Pro Thr Ser Asn Val Glu Ala Leu Lys Thr Leu Pro Lys Glu lie 305 310 315 320

Thr Glu Asp Pro Ala lie Tyr Pro Thr Ala Asp Val Leu Lys Ala Ala 325 330 335

Gin Trp Gin Asp Asp Val Gly Asn Ala lie Glu Leu Tyr GXu Lys 340 345 350 <210> 158 <211> 525 <212> DNA <213>昀膜肺炎故蟑；?早谄 <220> <223> rpmF <220> <22l> CDS <222> (1).. (522) <400> 158 atg caa aag gta aaa eta ccc etc acc att gac cca tat aaa gac get 48 Met Gin Lys Val Lys Leu Pro Leu Thr He Asp Pro Tyr Lys Asp Ala 1 5 10 15 cag cgt cga atg gat tac gaa ggc tac ate tea cgt agt ctg ett aat 96 Gin Arg Arg Met Asp Tyr Glu Gly Tyr lie Ser Arg Ser Leu Leu Asn 20 25 30 cgt ttg ggt gaa tet gtg age aat gtg eta age gat gca caa gtt act 144 Arg Leu Gly Glu Ser Val Ser Asn Val Leu Ser Asp Ala Gin Val Thr 35 40 45 etc teg tta tat ate gat ccg caa ege tta acc gtt att aaa ggt aeg 192 Leu Ser Leu Tyr lie Asp Pro Gin Arg Leu Thr Val lie Lys Gly Thr 50 55 60 geg aca gtg gaa gtg gaa ttc gat tgc caa cga tgc ggt aac ccg ttt 240

•253 · 136852-序列表.doc 111 2881334884

Ala Thr Val Glu Val Glu Phe Asp Cys Gin Arg Cys Gly Asn Pro Phe 65 70 75 80 aca caa acg ctt gac tgt teg ttt tgt ttc agt ccg gtg tcc aat atg Thr Gin Thr Leu Asp Cys Ser Phe Cys Phe Ser Pro Val Ser Asn Met 85 90 95 gat cag geg gac aat ttg ccc gaa att tat gaa cca ate gaa gta aac Asp Gin Ala Asp Asn Leu Pro Glu lie Tyr Glu Pro lie Glu Val Asn 100 105 110 gag ttc ggt gaa gta aat tta eta gat atg ate gaa gat gga ttt ate Glu Phe Gly Glu Val Asn Leu Leu Asp Met lie Glu Asp Gly Phe lie 115 120 125 ate gaa ttg cct eta gtc ccg atg cat agt gaa gaa cac tgt gaa gtg lie Glu Leu Pro Leu Val Pro Met His Ser Glu Glu His Cys Glu Val 130 135 140 tcc gtg agt gaa cag gtg ttt ggc gaa ttg cct gaa gaa ttg geg aaa Ser Val Ser Glu Gin Val Phe Gly Glu Leu Pro Glu Glu Leu Ala Lys 145 150 155 160 aaa cct aac ccg ttc get gta tta get aat tta aag aaa aac tag Lys Pro Asn Pro Phe Ala Val Leu Ala Asn Leu Lys Lys Asn 165 170 336 364 432 480 525 <210> 159 <211> 174 <212>蛋白贫 <213:>胸膜肺炎放線桿菌 <400> 159

Met Gin Lys Val Lys Leu Pro Leu Thr lie Asp Pro Tyr Lys Asp Ala 1 5 10 15

Gin Arg Arg Met Asp Tyr Glu Gly Tyr lie Ser Arg Ser Leu Leu Asn 20 25 30

Arg Leu Gly Glu Ser Val Ser Asn Val Leu Ser Asp Ala Gin Val Thr 35 40 45

Leu Ser Leu Tyr lie Asp Pro Gin Arg Leu Thr Val lie Lys Gly Thr 50 55 60

Ala Thr Val Glu Val Glu Phe Asp Cys Gin Arg Cys Gly Asn Pro Phe 65 70 75 80

Thr Gin Thr Leu Asp Cys Ser Phe Cys Phe Ser Pro Val Ser Asn Met 85 90 95

Asp Gin Ala Asp Asn Leu Pro Glu lie Tyr Glu Pro He Glu Val Asn 100 105 110

Glu Phe Gly Glu Val Asn Leu Leu Asp Met lie Glu Asp Gly Phe lie 115 120 125 lie Glu Leu Pro Leu Val Pro Met His Ser Glu Glu His Cys Glu Val 130 135 140

Ser Val Ser Glu Gin Val Phe Gly Glu Leu Pro Glu Glu Leu Ala Lys 145 150 155 160 -254· 136852-序列表.doc

l SI 1334884

Lys Pro Asn Pro Phe Ala Val Leu Ala Asn Leu Lys Lys Asn 165 170

<210> 160 <211> 1302 <212> DNA <213>胸膜胂炎故線桿餡 <220> <223> tig <220> <221> CDS <222> (1) .. (1299) <400> 160 atg tea att tet att gaa act tta gaa ggc tta caa ege ege gta act 48

Met Ser lie Ser lie Glu Thr Leu Glu Gly Leu Gin Arg Arg Val Thr 15 10 15 att acc gta get get gat aaa ate gaa geg get tac aaa gag caa tta 96

lie Thr Val Ala Ala Asp Lys He Glu Ala Ala Tyr Lys Glu Gin Leu 20 25 30 aaa ggc tat geg aaa aac get cgt gta gac ggt ttc cgt aaa ggt aaa 144

Lys Gly Tyr Ala Lys Asn Ala Arg Val Asp Gly Phe Arg Lys Gly Lys 35 40 45 gta ccg cac gca att ate gaa caa cgt ttc ggt tta geg get ege caa 192

Val Pro His Ala lie lie Glu Gin Arg Phe Gly Leu Ala Ala Arg Gin 50 55 60 gac gta tta tee gat gaa atg caa cgt geg ttc ttt gat geg gta ate 240

Asp Val Leu Ser Asp Glu Met Gin Arg Ala Phe Phe Asp Ala Val lie 65 70 75 80 get gag aaa att aac ett gee ggt cgt cct acc ttc aca ccg aac aac 288

Ala Glu Lys lie Asn Leu Ala Gly Arg Pro Thr Phe Thr Pro Asn Asn 85 90 95 tac caa ccg agt caa gaa ttc age ttc act gca act ttt gaa gta ttc 336

Tyr Gin Pro Ser Gin Glu Phe Ser Phe Thr Ala Thr Phe Glu Val Phe 100 105 110

ccg gaa gtt gaa tta aaa ggc tta gaa aat ate gaa get gaa aaa ccg 384

Pro Glu Val Glu Leu Lys Gly Leu Glu Asn lie Glu Val Glu Lys Pro 115 120 125 gtt gta gaa ate aca gaa get gat tta gac aaa atg ate gat gtg tta 432

Val Val Glu He Thr Glu Ala Asp Leu Asp Lvs Met lie Asp Val Leu 130 135 140 cgt aaa caa caa geg act tgg get gaa tet caa gca geg gca caa geg 480

Arg Lys Gin Gin Ala Thr Trp Ala Glu Ser Gin Ala Ala Ala Gin Ala 145 150 155 160 gaa gac cgt gtt gta ate gac ttc gta ggt tet gta gac ggt gaa gag 528

Glu Asp Arg Val Val lie Asp Phe Val Gly Ser Val Asp Gly Glu Glu 165 170 * 175 ttt gaa ggc ggt aaa geg aca gac ttc act eta gca atg ggt caa agt 576

Phe Glu Gly Gly Lys Ala Thr Asp Phe Thr Leu Ala Met Gly Gin Ser •255 · 】36852-序列表.doe 131 1334884 180 1Θ5 190 cgt atg ate cct ggt ttt gaa gaa ggt ate gtt ggt cac aaa gee ggc 624 Arg Met lie Pro Gly Phe Glu Glu Gly lie Val Gly His Lys Ala Gly 195 200 205 gaa caa ttc gat ate gat gtt act ttc cct gaa gaa tac cac get gaa 672 Glu Gin Phe Asp lie Asp Val Thr Phe Pro Glu Glu Tyx His Ala Glu 210 215 220 aac tta aaa ggt aaa geg geg aaa ttc gca att aca ett aag aaa gta 720 Asn Leu Lys Gly Lys Ala Ala Lys Phe Ala lie Thr Leu Lys Lys Val 225 230 235 240 gaa aat ate gta tta cct gaa tta acc gaa gaa ttc gtg aaa aaa ttc 768 Glu Asn lie Val Leu Pro Glu Leu Thr Glu Glu Phe Val Lys Lys Phe 245 250 255 ggt tea gca aaa act gta gaa gat tta cgt geg gaa att aag aaa aat 816 Gly Ser Ala Lys Thr Val Glu Asp Leu Arg Ala Glu lie Lys Lys Asn 260 265 270

atg caa cgt gaa ett aaa aac gca gta acc gca ege gtt aaa aac caa 864 Met Gin Arg Glu Leu Lys Asn Ala Val Thr Ala Arg Val Lys Asn Gin 275 280 285 gta ate aac ggt tta ate gca caa aat gaa att gaa gtg ccg get gca 912 Val lie Asn Gly Leu lie Ala Gin Asn Glu lie Glu Val Pro Ala Ala 290 295 300 geg gta geg gaa gaa gtg gac gta tta cgt cgt caa geg gtt caa cgt 960 Ala Val Ala Glu Glu Val Asp Val Leu Arg Arg Gin Ala Val Gin Arg 305 310 315 320 ttc ggt ggt aaa ccg gaa atg get gca caa tta ccg geg gaa tta ttc 1008 Phe Gly Gly Lys Pro Glu Met Ala Ala Gin Leu Pro Ala Glu Leu Phe 325 330 335 gaa geg gat gca aaa cgt cgt gtt caa gta ggt tta tta ett tea acc 1056 Glu Ala Asp Ala Lys Arg Arg Val Gin Val Gly Leu Leu Leu Ser Thr 340 345 350 gta ate ggt act aac gaa tta aaa gtt gat gaa aaa cgt gtt gaa gaa 1104 Val lie Gly Thr Asn Glu Leu Lys Val Asp Glu Lys Arg Val Glu Glu 355 360 365

aeg att gca gaa ate get tea get tac gaa caa ccg geg gaa gtt gtt 1152 Thr He Ala Glu He Ala Ser Ala Tyr Glu Gin Pro Ala Glu Val Val 370 375 380 get cat tat geg aaa aac cgt caa tta acc gaa aat ate cgt aac gta 1200 Ala His Tyr Ala Lys Asn Arg Gin Leu Thr Glu Asn lie Arg Asn Val 385 390 395 400 gtg tta gaa gag caa geg gtt gaa gtt gta ett geg aaa gca aaa gta 1248 Val Leu Glu Glu Gin Ala Val Glu Val Val Leu Ala Lys Ala Lys Val 405 410 415 act gaa aaa geg act tet ttt gat gaa gta atg get caa caa get caa 1296 Thr Glu Lys Ala Thr Ser Phe Asp Glu Val Met Ala Gin Gin Ala Gin 420 425 430 ggc taa 1302 •256· 136852·序列表-doe

T SI 1334884

1 G 0 桴線放炎 t胂 6133謚 1 4 i W Λ Λ Λ Λ 0 12 3 1111 2 2 2 2 < < < < <400> 161

Met Ser lie Ser lie Glu Thr Leu Glu Gly Leu Gin Arg Arg Val Thr 15 10 15 lie Thr Val Ala Ala Asp Lys lie Glu Ala Ala Tyr Lys Glu Gin L<eu 20 25 30

Lys Gly Tyr Ala Lys Asn Ala Arg Val Asp Gly Phe Arg Lys Gly Lys 35 40 45

Val Pro His Ala lie lie Glu Gin Arg Phe Gly Leu Ala Ala Arg Gin 50 55 60

Asp Val Leu Ser Asp Glu Met Gin Arg Ala Phe Phe Asp Ala Val lie 65 70 75 80

Ala Glu Lys lie Asn Leu Ala Gly Arg Pro Thr Phe Thr Pro Asn Asn Θ5 90 95

Tyr Gin Pro Ser Gin Glu Phe Ser Phe Thr Ala Thr Phe Glu Val Phe 100 105 110

Pro Glu Val Glu Leu Lys Gly Leu Glu Asn lie Glu Val Glu Lys Pro 115 120 125

Val Val Glu lie Thr Glu Ala Asp Leu Asp Lys Met lie Asp Val Leu 130 135 140

Arg Lys Gin Gin Ala Thr Trp Ala Glu Ser Gin Ala Ala Ala Gin Ala 145 150 155 160

Glu Asp Arg Val Val lie Asp Phe Val Gly Ser Val Asp Gly Glu Glu 165 170 175

Phe Glu Gly Gly Lys Ala Thr Asp Phe Thr Leu Ala Met Gly Gin Ser 180 185 190

Arg Met lie Pro Gly Phe Glu Glu Gly *Ile Val Gly His Lys Ala Gly 195 200 205

Glu Gin Phe Asp lie Asp Val Thr Phe Pro Glu Glu Tyr His Ala Glu 210 215 220

Asn Leu Lys Gly Lys Ala Ala Lys Phe Ala lie Thr Leu Lys Lys Val 225 230 235 240

Glu Asn lie Val Leu Pro Glu Leu Thr Glu Glu Phe Val Lys Lys Phe 245 250 255

Gly Ser Ala Lys Thr Val Glu Asp Leu Arg Ala Glu He Lys Lys Asn 260 265 270

Met Gin Arg Glu Leu Lys Asn Ala Val Thr Ala Arg Val Lys Asn Gin 275 280 285 •257- 136852-序列表.d〇c si 1334884

Val lie Asn Gly Leu lie Ala Gin Asn Glu lie Glu Val Pro Ala Ala 290 295 300

Ala Val Ala Glu Glu Val Asp Val Leu Arg Arg Gin Ala Val Gin Arg 305 310 315 320

Phe Gly Gly Lys Pro Glu Met Ala Ala Gin Leu Pro Ala Glu Leu Phe 325 330 335

Glu Ala Asp Ala Lys Arg Arg Val Gin Val Gly Leu Leu Leu Ser Thr 340 345 350

Val lie Gly Thr Asn Glu Leu Lys Val Asp Glu Lys Arg Val Glu Glu 355 360 365

Thr lie Ala Glu lie Ala Ser Ala Tyr Glu Gin Pro Ala Glu Val Val 370 375 360

Ala His Tyr Ala Lys Asn Arg Gin Leu Thr Glu Asn lie Arg Asn Val 385 390 395 400

Val Leu Glu Glu Gin Ala Val Glu Val Val Leu Ala Lys Ala Lys Val

405 410 415

Thr Glu Lys Ala Thr Ser Phe Asp Glu Val Met Ala Gin Gin Ala Gin 420 425 430

Gly

<210> 162 <211> 316 <212> DNA <213 >胸膜肺炎故線桿菌 c220> <223> tRNA-glu <400> 162 aatattgcgc tcaaatggca aagcggagag catctttaaa tgttgtcccc atcgtctaga 60 ggcctaggac atcgcccttt cacggcggta accggggttc gaatccccgt ggggacgcca 120 tttaaagatg acttttgttg tctgaattgt tctttaaaaa attggaaaca agctgaaaac 180

tgagagattt tcgaaagaaa gtctgagtag taaaagataa gtaattatct tgaaaa.tctt 240 agctgaacaa aagcagctaa gtgtttagtt gaataaagta tcgcgttgaa tgcgttcaaa 300 taaaatttga aaatat 316

<210> 163 <2X1> 85 <212> DNA <213>胸膜肺炎放線桿菌 <220> <223> tRNA-leu <4 00> 163 gctccggtgg tggaattggt agacacgcta tcctgagggg gtagtatcca taggatgtgc 60 •258· 136852-序列表.doc i si 1334884 85 gagttcgagt ctcgcccaga gcacc

<210> 164 <211> 623 <212> DNA <213>詾膜肺炎故線桿菌 <220>

<223> yaeE <220> <221> CDS <222> (1) . . (621) <400> 164 atg caa gaa etc aca cct caa atg tgg ggc tta gtc ggc act tea aeg 48

Met Gin Glu Leu Thr Pro Gin Met Trp Gly Leu Val Gly Thr Ser Thr 15 i〇 15 ett gaa aeg etc tat atg ggc ttt geg geg act tta ett get gtg gta 96

Leu Glu Thr Leu Tyr Met Gly Phe Ala Ala Thr Leu Leu Ala Val Val 20 25 30 gtc ggt ttg ccg ate ggt ttt ctg gca ttt tta acc ggt aaa gga gng 144

Val Gly Leu Pro lie Gly Phe Leu Ala Phe Leu Thr Gly Lys Gly Glu 35 40 45 att tta gag aat ccg cgt tta cat caa gta tta gat gtg att att aat 192

He Leu Glu Asn Pro Arg Leu His Gin Val Leu Asp Val lie lie Asn 50 55 60 ate ggt cgt tcc gta ccg ttt att att ttg tta gtc gtg ttg tta cct 240

He Gly Arg Ser Val Pro Phe lie He Leu Leu Val Val Leu Leu Pro 65 70 75 80 ttt aeg cgt tta ttg gtc ggg aca aeg etc ggt act aeg geg geg att 288

Phe Thr Arg Leu Leu Val Gly Thr Thr Leu Gly Thr Thr Ala Ala He 85 90 95 gtg ccg tta age gtt teg gca att ccg ttt ttt geg cgt tta act tea 336

Val Pro Leu Ser Val Ser Ala lie Pro Phe Phe Ala Arg Leu Thr Ser 100 105 no

aat geg tta tta gaa ate cca gca ggt tta acc gaa geg geg aaa teg 384

Asn Ala Leu Leu Glu lie Pro Ala Gly Leu Thr Glu Ala Ala Lys Ser 115 120 125 atg ggc gca aeg aat tgg caa gtg gtc agt aaa ttt tat tta ccg gaa 432

Met Gly Ala Thr Asn Trp Gin Val Val Ser Lys Phe Tyr Leu Pro Glu 130 135 140 tea ctg ccg att tta ate aat ggt ate aca tta act tta gtc get tta 480

Ser Leu Pro lie Leu lie Asn Gly lie Thr Leu Thr Leu Val Ala Leu 145 150 155 160 ate ggt tat teg gca atg geg ggt geg gtc ggc ggc ggc ggt ttg ggt 528 lie Gly Tyr Ser Ala Met Ala Gly Ala Val Gly Gly Gly Gly Leu Gly 165 170 175 aac ett gee ate agt tac ggt gaa cac ega aat atg gtc tat gta aaa 576

Asn Leu Ala He Ser Tyr Gly Glu His Arg Asn Met Val Tyr Val Lys 180 185 i9〇 • 259· 136852·序列表.doc IS] 6231334884 tgg ate tea aca att att ate gta geg att gtg atg ate agt caa aa Trp lie Ser Thr lie lie lie Val Ala lie Val Met lie Ser Gin 195 2〇〇 205 <210> 165 <211> 207 <212>蛋白質 <213>胸骐肺.¾故線桿谪 <400> 165

Met Gin Glu Leu Thr Pro Gin Met Trp Gly Leu Val Gly Thr Ser Thr 1 5 i〇 15

Leu Glu Thr Leu Tyr Met Gly Phe Ala Ala Thr Leu Leu Ala Val Val 20 25 30

Val Gly Leu Pro lie Gly Phe Leu Ala Phe Leu Thr Gly Lys Gly Glu 35 40 45 lie Leu Glu Asn Pro Arg Leu His Gin Val Leu Asp Val lie lie Asn

50 55 60 lie Gly Arg Ser Val Pro Phe lie lie Leu Leu Val Val Leu Leu Pro 65 70 75 80

Phe Thr Arg Leu Leu Val Gly Thr Thr Leu Gly Thr Thr Ala Ala lie 85 90 95

Val Pro Leu Ser Val Ser Ala lie Pro Phe Phe Ala Arg Leu Thr Ser 100 105 110

Asn Ala Leu Leu Glu He Pro Ala Gly hen Thr Glu Ala Ala Lys Ser 115 120 125

Met Gly Ala Thr Asn Trp Gin Val Val Ser Lys Phe Tyr Leu Pro Glu 130 135 140

Ser Leu Pro He Leu lie Asn Gly lie Thr Leu Thr Leu Val Ala Leu 145 150 155 160

He Gly Tyr Ser Ala Met Ala Gly Ala Val Gly Gly Gly Gly Leu Gly 165 170 175

Asn Leu Ala lie Ser Tyr Gly Glu His Arg Asn Met Val Tyr Val Lys 180 185 190

Trp lie Ser Thr lie lie lie Val Ala lie Val Met lie Ser Gin 195 200 205

<210> 166 <211> Θ66 <212> DNA <213> ;•容血性巴斯德桿谨 <220> <221> CDS <222> (1) . . (864) <220>

<223> atpG 136852·序列表.doc -260- 1^1 481334884

<400> 166 atg gca gqt get aaa gag ata aga acc aaa att gca aat get cat aat Met Ala Gly Ala Lys Glu Tie Ara Thr Lys lie Ala Ser Val Arg Asn 1 5 " 10 15 aca caa aaa att acc aaa geg atg gaa atg gtt gee gca tea aaa atg Thr Gin Lys lie Thr Lys Ala Met Glu Met Val Ala Ala Ser Lys Met 20 25 30 cgt aaa acc caa gag cgt atg geg get tet eye cct tat get gaa agt Arg Lys Thr Gin Glu Arg Met Ala Ala Ser Arg Pro Tyr Ala Glu Ser 35 40 45 att ege aag gca att age cat att gee aaa ggt aac att gag tat aaa lie Arg Lys Ala lie Ser His lie Ala Lys Gly Asn lie Glu Tyr Lys 50 55 60 cac cca ttt ttg acc cca cgt ccg gta aaa aaa gtt ggc tat tta gta His Pro Phe Leu Thr Pro Arg Pro Val Lys Lys Val Glv Tyr Leu Val 65 70 75 80 gtt tea acc gat ege ggt tta tgt ggt ggc tta aat ate aat tta ttt Val Ser Thr Asp Arg Gly Leu Cys Glv Gly Leu Asn lie Asn Leu Phe 85 90 95 aaa acc gtt tta cat gaa ttg aaa gaa aaa gat gac caa ggt gtt aag Lys Thr Val Leu His Glu Leu Lys Glu Lys Asp Asp Gin Gly Val Lys 100 105 110 tet ega ett get gtg gtg gga aat aaa ggg ate tee ttt ttt aac cca Ser Arg Leu Ala Val Val Gly Asn Lys Gly lie Ser Phe Phe Asn Pro 115 120 125 atg ggg eta gag att aaa ggt cat ate aat gga ttg ggt gat aca ccg Met Gly Leu Glu lie Lys Gly His lie Asn Gly Leu Gly Asp Thr Pro 130 135 140 " gca atg gaa gat tta gtc ggt att gtt aat ggt atg gta aat gee tac Ala Met Glu Asp Leu Val Gly He Val Asn Gly Met Val Asn Ala Tyr 145 150 155 160 cgt gaa ggc gaa att gat gaa gtg tat gtg gta tat aac cgt ttt ata Arg Glu Gly Glu lie Asp Glu Val Tyr Val Val Tyr Asn Arg Phe He 165 170 175 aac aeg atg tea caa aaa ccg aca gta caa cag ttg ett cct ttg cct Asn Thr Met Ser Gin Lys Pro Thr Val Gin Gin Leu Leu Pro Leu Pro 180 185 190 gca ctg gaa aat gac tea tta gag caa act ggt tet tgg gat tat etc Ala Leu Glu Asn Asp Ser Leu Glu Gin Thr Gly Ser Trp Asp Tyr Leu 195 200 205 tat gaa cca aat cca caa geg tta tta gac age tta ctg gtc cgt tat Tyr Glu Pro Asn Pro Gin Ala Leu Leu Asp Ser Leu Leu Val Arg Tyr 21〇 215 220 tta gaa tet caa gtt tat cag gca gtg gta gat aat etc geg tet gaa Leu Glu Ser Gin Val Tyr Gin Ala Val Val Asp Asn Leu Ala Ser Glu 225 230 235 240 cag get get ega atg gtg gca atg aaa gca gca acc gat aac gca ggt Gin Ala Ala Arg Met Val Ala Met Lys Ala Ala Thr Asp Asn Ala Gly 96 144 192 240 2Θ8 336 384 432 480 528 576 624 672 720 136852-序列表.doc -261 - 131 768 1334884 245 250 255 aat ctg att aat gag tta cag tta gtg tat aac aaa get cgt caa gca 81β

Asn Leu lie Asn Glu Leu Gin Leu Val Tyr Asn Lys Ala Arg Gin Ala 260 265 270 agt att aeg aat gaa tta aat gaa att gtc geg ggt gec gca gca att 864

Ser lie Thr Asn Glu Leu Asn Glu He Val Ala Gly Ala Ala Ala lie 275 280 285 ta <21〇> 167 <211> 288 <212> 蛋白質 866 <213> :·容jk性巴期德桿菌 <400> 167

Met Ala Gly Ala Lys Glu lie Arg Thr Lys lie Ala Ser Val Arg Asn 15 10 15

Thr Gin Lys lie Thr Lys Ala Met Glu Met Val Ala Ala Ser Lys Met 20 25 30

Arg Lys Thr Gin Glu Arg Met Ala Ala Ser Arg Pro Tyr Ala Glu Ser 35 40 45 lie Arg Lys Ala lie Ser His lie Ala Lys Gly Asn lie Glu Tyr Lys 50 55 60

His Pro Phe Leu Thr Pro Arg Pro Val Lys Lys Val Gly Tyr Leu Val 65 70 75 80

Val Ser Thr Asp Arg Gly Leu Cys Gly Gly Leu Asn lie Asn Leu Phe 85 90 95

Lys Thr Val Leu His Glu Leu Lys Glu Lys Asp Asp Gin Gly Val Lys 100 105 110

Ser Arg Leu Ala Val Val Gly Asn Lys Gly lie Ser Phe Phe Asn Pro 115 120 125

Met Gly Leu Glu lie Lys Gly His lie Asn Gly Leu Gly Asp Thr Pro 130 135 140

Ala Met Glu Asp Leu Val Gly lie Val Asn Gly Met Val Asn Ala Tyr 145 ISO 155 160

Arg Glu Gly Glu lie Asp Glu Val Tyr Val Val Tyr Asn Arg Phe lie 165 170 175

Asn Thr Met Ser Gin Lys Pro Thr Val Gin Gin Leu Leu Pro Leu Pro 180 185 190

Ala Leu Glu Asn Asp Ser Leu Glu Gin Thr Gly Ser Trp Asp Tyr Leu 195 200 205

Tyr Glu Pro Asn Pro Gin Ala Leu Leu Asp Ser Leu Leu Val Arg Tyr 210 215 220

Leu Glu Ser Gin Val Tyr Gin Ala Val Val Asp Asn Leu Ala Ser Glu 225 230 235 240 262- 136852-序列表.doc t si 1334884

Gin Ala Ala Arg Met Val Ala Met Lys Ala Ala Thr Asp Asn Ala Gly 245 250 255

Asn Leu lie Asn Glu Leu Gin Leu Val Tyr Asn Lys Ala Arg Gin Ala 260 265 270

Ser lie Thr Asn Glu Leu Asn Glu lie Val Ala Gly Ala Ala Ala lie 275 280 285

<210> 168 <211> 1463 <212> DNA <213> :容血性巴斯德.桴涵 <220> <221> CDS <222> (1) .. (1461) <220>

<223> guaB

<400> 168 atg eta ega att aaa caa gaa gcc etc act ttt gat gat gtt ett etc 48 Met 1 Leu Arg lie Lys 5 Gin Glu Ala Leu Thr 10 Phe Asp Asp Val Leu 15 Leu gtc ccg gca cat tet act gtg ett cct aat act get gat ett tet act 96 Val Pro Ala His 20 Ser Thr Val Leu Pro 25 Asn Thr Ala Asp Leu 30 Ser Thr caa tta act aaa acc att cgt tta aac att ccg atg ett tet get gca 144 Gin Leu Thr 35 Lys Thr lie Arg Leu 40 Asn He Pro Met Leu 45 ser Ala Ala atg gat acc gtt aca gaa act aag ett geg ate tcc ett get caa gaa 192 Met Asp 50 Thr Val Thr Glu Thr 55 Lys Leu Ala lie Ser 60 Leu Ala Gin Glu ggc ggc att ggt ttt ate cat aaa aat atg teg att gaa ege cag gca 240 Gly Gly 65 lie Gly Phe lie 70 His Lys Asn Met Ser 75 lie Glu Arg Gin Ala 80 gac cgt gtg cgt aaa gtg aaa aaa ttt gaa agt ggt att gtt tet gag 288 Asp Arg val Arg Lys Θ5 Val Lys Lys Phe Glu 90 Ser Gly He Val Ser 95 Glu cca gtg aeg att tet cct gat atg aca tta geg gaa ttg get gaa ttg 336 Pro Val Thr lie 100 Ser Pro Asp Met Thr 105 Leu Ala Glu Leu Ala 110 Glu Leu gtg aaa aag aac ggt ttt gca ggc tat ccg gtg att gat gaa aac caa 384 Val Lys Lys 115 Asn Gly Phe Ala Gly Tyr 120 Pro Val lie Asp 125 Glu Asn Gin aat tta gtg gga att att acc gga cgt gat acc ega ttt gtc aeg gat 432 Asn Leu 130 Val Gly lie lie Thr Gly 135 Arg Asp Thr Arg 140 Phe Val Thr Asp cca age aaa aca gtg cgt gaa ttt atg aca cca aaa gac cgt tta gtg 480 Leu 145 Ser Lys Thr Val Arg 150 Glu Phe Met Thr Pro 155 Lys Asp Arg Leu Val 160 -263· 136852·序列表.doc ί si 1334884 acg gta aaa gaa aac gca age cgt gaa gaa att ttc cac tta atg cac 528

Thr Val Lys Glu Asn Ala Ser Arg Glu Glu lie Phe His Leu Met His 165 170 175 gaa cac ega gtg gag aaa gtg ctg gta gtg aat aat gaa ttt cag tta 576

Glu His Arg Val Glu Lys Val Leu Val Val Asn Asn Glu Phe Gin Leu 180 185 190 aaa gga atg att acc eta aaa gac tac caa aaa geg gaa age aaa ccg 624

Lys Gly Met lie Thr Leu Lys Asp Tyr Gin Lys Ala Glu Ser Lys Pro 195 200 205 aat gcc tgt aaa gat gag ttt ggg cgt ttg cgt gtg ggg geg gca gtg 672

Asn Ala Cys Lys Asp Glu Phe Gly Arg Leu Arg Val Gly Ala Ala Val 210 215 220 gga gcc ggt ccg ggc aat gaa gaa ega att gat get tta gta aaa geg 720

Gly Ala Gly Pro Gly Asn Glu Glu Arg lie Asp Ala Leu Val Lys Ala 225 230 235 240 ggt gtc gat gtg eta tta ate gac tet teg cac ggg cat tet gaa ggt 768

Gly Val Asp Val Leu Leu lie Asp Ser Ser His Gly His Ser Glu Gly 245 250 255 gta tta caa cgt gtg cgt gaa acc cgt gca aaa tac cct gat tta ccg 816

Val Leu Gin Arg Val Arg Glu Thr Arg Ala Lys Tyr Pro Asp Leu Pro 260 265 270 att gtt gcc ggt aat att gcc act gca gaa gga geg att geg tta get 864 lie Val Ala Gly Asn lie Ala Thr Ala Glu Gly Ala lie Ala Leu Ala 275 2B0 285 gat gca gga gcc agt get gtg aaa gta gga ate ggc ccg ggt tea att 912

Asp Ala Gly Ala Ser Ala Val Lys Val Gly lie Gly Pro Gly Ser lie 290 295 300 tgt acc acc aga att gta aca ggc gtt ggc gtg cca caa ate acg gca 960

Cys Thr Thr Arg lie Val Thr Gly Val Gly Val Pro Gin lie Thr Ala 305 310 315 320 ate gca gaa geg gca get geg ett aaa gaa ega ggc att cct gtg att 1008 lie Ala Glu Ala Ala Ala Ala Leu Lys Glu Arg Gly lie Pro Val lie 325 330 335

get gat ggt gga att cgt tat tea ggc gat att tea aaa get att gcc 1056

Ala Asp Gly Gly lie Arg Tyr Ser Gly Asp lie Ser Lys Ala lie Ala 340 345 350 gee ggt gca agt tgc gta acg gtc ggt teg atg ett gcc ggc aca gaa 1104

Ala Gly Ala Ser Cys Val Met Val Gly Ser Met Phe Ala Gly Thr Glu 355 360 365 gaa gcc ccg ggt gaa att gag ett tat caa ggc aga gca ttc aaa tee 1152

Glu Ala Pro Gly Glu lie Glu Leu Tyr Gin Gly Arg Ala Phe Lys Ser 370 375 380 tac cgt gga atg gga tea tea ggt gca atg agt aaa ggc teg tea gat 1200

Tyr Arg Gly Met Gly Ser Leu Gly Ala Met Ser Lys Gly Ser Ser Asp 385 390 395 400 ege tat ttc caa tet gat aat gcc gcc gac aag etc gta ccg gaa ggg 1248

Arg Tyr Phe Gin Ser Asp Asn Ala Ala Asp Lys Leu Val Pro Glu Gly 405 410 415 -264- 136852-序列表,doc i 12961334884 att gaa ggg cgt ate get tac aaa ggc tac ttg aaa gaa att ate cac lie Glu Gly Arg lie Ala Tyr Lys Gly Tyr Leu Lys Glu lie lie His 420 425 430 caa caa atg ggc ggc tta ege tee tgt atg gga tta acc ggc tgt gee Gin Gin Met Gly Gly Leu Arg Ser Cys Met Gly Leu Thr Gly Cys Ala 435 440 445 act att gaa gaa etc ege acc aaa gca gaa ttt gtc ege att agt ggt Thr lie Glu Glu Leu Arg Thr Lys Ala Glu Phe Val Arg lie Ser Gly 450 455 460 get ggt att aaa gaa age cac gtc cac gat gtg aca att acc aaa gaa Ala Gly He Lys Glu Ser His Val His Asp Val Thr lie Thr Lys Glu 465 470 475 480 gca ccg aac tac ega atg ggt ta Ala Pro Asn Tyr Arg Met Gly 485 1344 1392 1440 1463

<210> 169 <211> 487 <212>蛋白質 <213>溶血性巴斯德桴简 . <400> 169

Met Leu Arg lie Lys Gin Glu Ala Leu Thr Phe Asp Asp Val Leu Leu 15 10 15

Val Pro Ala His Ser Thr Val Leu Pro Asn Thr Ala Asp Leu Ser Thr 20 25 30

Gin Leu Thr Lys Thr lie Arg Leu Asn lie Pro Met Leu Ser Ala Ala 35 40 45

Met Asp Thr Val Thr Glu Thr Lys Leu Ala lie Ser Leu Ala Gin Glu 50 55 60

Gly Gly He Gly Phe lie His Lys Asn Met Ser lie Glu Arg Gin Ala 65 70 75 80

Asp Arg Val Arg Lys Val hys Lys Phe Glu Ser Gly lie Val Ser Glu 85 90 95

Pro Val Thr lie Ser Pro Asp Met Thr Leu Ala Glu Leu Ala Glu Leu 100 105 110

Val Lys Lys Asn Gly Phe Ala Gly Tyr Pro Val He Asp Glu Asn Gin 115 120 125

Asn Leu Val Gly lie lie Thr Gly Arg Asp Thr Arg Phe Val Thr Asp 130 135 140

Leu Ser Lys Thr Val Arg Glu Phe Met Thr Pro Lys Asp Arg Leu Val 145 150 155 ' 160

Thr Val Lys Glu Asn Ala Ser Arg Glu Glu He Phe His Leu Met His 165 170 175

Glu His Arg Val Glu Lys Val Leu Val Val Asn Asn Glu Phe Gin Leu 180 185 i9〇 -265- 136852·序列表.doc IZ1 1334884

Lys Gly Met lie Thr Leu Lys Asp Tyr Gin Lys Ala Glu Ser Lys Pro 195 200 205

Asn Ala Cys Lys Asp Glu Phe Gly Arg Leu Arg Val Gly Ala Ala Val 210 215 220

Gly Ala Gly Pro Gly Asn Glu Glu Arg He Asp Ala Leu Val Lys Ala 225 230 235 240

Gly Val Asp Val Leu Leu He Asp Ser Ser His Gly His Ser Glu Gly 245 250 255

Val Leu Gin Arg Val Arg Glu Thr Arg Ala Lys Tyr Pro Asp Leu Pro 260 265 270 lie Val Ala Gly Asn lie Ala Thr Ala Glu Gly Ala lie Ala Leu Ala 275 280 285

Asp Ala Gly Ala Ser Ala Val Lys Val Gly lie Gly Pro Gly Ser lie 290 295 300 "

Cys Thr Thr Arg lie Val Thr Gly Val Gly Val Pro. Gin lie Thr Ala 305 310 3X5 320 lie Ala Glu Ala Ala Ala Ala Leu Lys Glu Arg Gly lie Pro Val lie 325 330 335

Ala Asp Gly Gly lie Arg Tyr Ser Gly Asp lie Ser Lys Ala lie Ala 340 345 350

Ala Gly Ala Ser Cys Val Met Val Gly Ser Met Phe Ala Gly Thr Glu 355 360 365

Glu Ala Pro Gly Glu lie Glu Leu Tyr Gin Gly Arg Ala Phe Lys Ser 370 375 380

Tyr Arg Gly Met Gly Ser Leu Gly Ala Met Ser Lys Glv Ser Ser Asp 385 390 395 400

Arg Tyr Phe Gin Ser Asp Asn Ala Ala Asp Lys Leu Val Pro Glu Gly 405 410 415

lie Glu Gly Arg lie Ala Tyr Lys Gly Tyr Leu Lys Glu lie lie His 420 425 430

Gin Gin Met Gly Gly Leu Arg Ser Cys Met Gly Leu Thr Gly Cys Ala 435 440 445

Thr He Glu Glu Leu Arg Thr Lys Ala Glu Phe Val Arg lie Ser Gly 450 455 460

Ala Gly lie Lys Glu Ser His Val His Asp Val Thr lie Thr Lys Glu 465 470 475 480

Ala Pro Asn Tyr Arg Met Gly 485

<210> 170 <211> 2150 <212> DNA <213>涫血性巴斯德；(羊菌 -266· 136852-jf^.doc 131 1334884 <220> <221> CDS <222> (1) . . (2148) <220> <223> pnp

<400> 170 atg act cca att gta aaa cag ttt aaa tac ggt cag cac acc gtg acc 48 Met Thr Pro lie Val Lys Gin ϊ .ie Lys Tyr Gly Gin His Thr Val Thr 1 5 10 15 tta gaa acc ggt get ate gca ege caa gca aeg gca gca gta atg gca 96 Leu Glu Thr Gly Ala lie Ala Arg Gin Ala Thr Ala Ala Val Met Ala 20 25 30 agt atg gac gac aca acc gta ttt gtt acc gta gta gcg aaa aaa gac 144 Ser Met Asp Asp Thr Thr Val Phe Val Thr Val Val Ala Lys Lys Asp 35 40 45 gta aaa gaa ggg caa gat ttc ttc cca tta acc gta gat tat caa gag 192 Val Lys Glu Gly Gin Asp Phe Phe Pro Leu Thr Val Asp Tyr Gin Glu 50 55 60 cgt act tac gca gcc ggt cgt act ccg ggc ggt ttc ttc aaa cgt gaa 240 Arg Thr Tyr Ala Ala Gly Arg lie Pro Gly Gly Phe Phe Lys Arg Glu 65 70 75 80 gga cgt cct age gaa ggt gaa acc tta ate get ege ttg ate gac cgt 2ΘΘ Gly Arg Pro Ser Glu Gly Glu Thr Leu lie Ala Arg Leu lie Asp Arg 85 90 95 cct gtg cgt cca ett ttc cca gaa ggt ttc ttt aac gaa att caa gtg 336 Pro Val Arg Pro Leu Phe Pro Glu Gly Phe Phe Asn Glu lie Gin Val 100 105 110 att gcg acc gta gta teg gta aac cca caa ate agt cct gat ctg gtt 384 lie Ala Thr Val val Ser Val Asn Pro Gin lie Ser Pro Asp Leu val 115 120 125 gcg atg ate ggt gca teg get gcc ett tea tta tee ggc gtg ccg ttt 432 Ala Met lie Gly Ala Ser Ala Ala Leu Ser Leu Ser Gly Val Pro Phe 130 135 140 aac ggt cca ate ggt gcg get cgt gtc ggt ttt ate aac gat caa ttc 480 Asn Gly Pro lie Gly Ala Ala Arg val Gly Phe lie Asn Asp Gin Phe 145 150 155 160 gta tta aac cca acc acc age gag caa aaa ate age ege tta gat tta 52β Val Leu Asn Pro Thr Thr Ser Glu Gin Lys lie Ser Arg Leu Asp Leu 165 170 175 gtg gtt tea ggt aca gac aaa gcc gtg ttg atg gtg gaa tet gaa gcg 576 Val Val Ser Gly Thr Asp Lys Ala Val Leu Met Val Glu Ser Glu Ala 180 185 190 gat ate tta acc gaa gag caa atg tta gcg gcg gtg gtg ttc ggc cac 624 Asp lie Leu Thr Glu Glu Gin Met Leu Ala Ala Val Val Phe Gly His 195 200 205 gag caa caa cag gtt gta ate gaa aac ate aaa gaa ttt gtt aaa gaa 672 Glu Gin Gin Gin Val Val lie Glu Asn lie Lys Glu Phe Val Lys Glu 210 215 220 136852·序列表.doc • 267· 7201334884

gcg ggc aaa cca cgt tgg gat tgg gtt gca cca gag cca aat aca gat Ala Gly Lys Pro Arg Trp Asp Trp Val Ala Pro Glu Pro Asn Thr Asp 225 230 235 240 tta ate aac aaa gta aaa gca tta gca gaa aca ege ett ggc gat get Leu He Asn Lys Val Lys Ala Leu Ala Glu Thr Arg Leu Gly Asp Ala 245 250 255 tat cgt ate gta gaa aaa caa gtt cgt tac gag caia ate gat gcg att ryr Arg lie Val Glu Lys Gin Val Arg Tyr Glu Gin lie Asp Ala lie 260 265 270 aaa gca gag gtg att gca caa ett acc gca gaa gat gaa act gtt tet Lys Ala Glu Val lie Ala Gin Leu Thr Ala Glu Asp Glu Thr Val Ser 275 280 285 gaa ggg act ate ate gac ate ate acc gca tta gag age caa ate gtg Glu Gly Thr lie lie Asp lie lie Thr Ala Leu Glu Ser Gin He Val 290 295 300 cgt age cgt att att gca ggc gaa cca ege att gac ggc cgt aeg gtg Arg Ser Arg He lie Ala Gly Glu Pro Arg lie Asp Gly Arg Thr Val 305 310 315 320 gat acc gtg cgt gca ttg gat att tgc acc agt gtg tta cca ege acc Asp Thr Val Arg Ala Leu Asp lie Cys Thr Ser Val Leu Pro Arg Thr 325 330 335 cac ggt tet get ett ttc acc cgt ggc gaa acc caa gca tta gca gta His Gly Ser Ala Leu Phe Thr Arg Gly Glu Thr Gin Ala Leu Ala Val 340 345 350 gca aca ttg ggc aca gag cgt gat gee caa ate att gac gaa ttg acc Ala Thr Leu Gly Thr Glu Arg Asp Ala Gin lie lie Asp Glu Leu Thr 355 360 365 ggc gaa aaa tet gac cgt ttc tta ttc cac tac aat ttc cct cca tac. Gly Glu Lys Ser Asp Arg Phe Leu Phe His Tyr Asn Phe Pro Pro Tyr 370 375 380 tet gtg ggc gaa acc ggt cgt ate ggc teg cca aaa ege cgt gaa ate Ser Val Gly Glu Thr Gly Arg lie Gly Ser Pro Lys Arg Arg Glu lie 385 390 395 400 ggt cac ggt cgt tta gca aaa cgt ggc gta tta gee gtg atg cca acc Gly His Gly Arg Leu Ala Lys Arg Gly Val Leu Ala Val Met Pro Thr 405 410 415 get gaa gag ttc ccg tat gta gtg cgt gtg gtg tet gaa ate act gaa Ala Glu Glu Phe Pro Tyr Val Val Arg Val Val Ser Glu lie Thr Glu 420 425 430 tet aac ggt tet tet tea atg gca tet gtg tgt ggt gcg tet etc gcg Ser Asn Gly Ser Ser Ser Met Ala Ser Val Cys Gly Ala Ser Leu Ala 435 440 445 ttg atg gac gca ggt gtg cca ate aaa gca gcg gtt gee ggt ate gca Leu Met Asp Ala Gly Val Pro He Lys Ala Ala Val Ala Gly He Ala 450 455 460 atg ggg etc gtg aaa gaa gac gag aaa ttc gtg gta ett tet gac ate Met Gly Leu Val Lys Glu Asp Glu Lys Phe Val Val Leu Ser Asp lie 465 470 475 4Θ0 768 816 B64 912 960 1008 1056 1104 1152 1200 1248 1296 1344 1392 136852-序列表.doc •268·

l SI 1440 1334884 tta ggt gat gaa gac cac tta ggc gat atg gac ttt aaa gta gcg gga 1488

Leu Gly Asp Glu Asp His Leu Gly Asp Met Asp Phe Lys Val Ala Gly 485 490 495 acc cgt acc ggt gtg act gcg ctg caa atg gac ate aaa ate gaa ggg 1536

Thr Arg Thr Gly Val Thr Ala Leu Gin Met Asp He Lys lie Glu Gly 500 505 510 ate acc cct gaa att atg cgt att gcc tta aac caa get aaa ggt gca 1584 lie Thr Pro Glu lie Met Arg lie Ala Leu Asn Gin Ala Lys Gly Ala 515 520 525 aga atg cac att tta ggt gta atg gaa caa gcc att ccg gca cct cgt 1632

Arg Met His lie Leu Gly Val Met Glu Gin Ala lie Pro Ala Pro Arg 530 535 540 gca gat att tet gac tat gcc cca ege att cac aca atg aag ate gat 1680

Ala Asp lie Ser Asp Tyr Ala Pro Arg lie His Thr Met Lys lie Asp 545 550 555 560 ccg aag aaa ate aaa gat gtg att ggt aaa ggc ggt gca aca att cgt 1728

Pro Lys Lys lie Lys Asp Val lie Gly Lys Gly Gly Ala Thr lie Arg 565 570 575 get tta acc gaa gag acc aat act tet ate gac att gat gat gac ggt 1776

Ala Leu Thr Glu Glu Thr Asn Thr Ser lie Asp lie Asp Asp Asp Gly 580 585 590 aeg gtg aaa att gcg gca act gac ggc aat gca gcg aaa gca gta atg 1824

Thr Val Lys lie Ala Ala Thr Asp Gly Asn Ala Ala Lys Ala Val Met 595 600 605 get cgt att gaa gag ate gtt gcc gaa gtg gaa gta aac caa ate tac 1872

Ala Arg lie Glu Glu lie Val Ala Glu Val Glu Val Asn Gin lie Tyr 610 615 620 aac ggt aaa gta acc cgt gtg gtg gac ttc ggt gca ttc gtt tec ate 1920

Asn Gly Lys Val Thr Arg Val Val Asp Phe Gly Ala Phe Val Ser lie 62S 630 635 640 tta ggt ggc aaa gaa ggt tta gtc cac att tea caa ate acc aac gaa 1968

Leu Gly Gly Lys Glu Gly Leu Val His lie Ser Gin lie Thr Asn Glu 645 650 655

cgt gtt gag cgt gta gcg gac tac tta acc gtt ggt caa gaa gta caa 2016

Arg Val Glu Arg Val Ala Asp Tyr Leu Thr Val Gly Gin Glu Val Gin 660 665 670 gtg aaa gtg gta gaa att gac cgt caa gga ege att cgt ctg aeg atg 2064

Val Lys Val Val Glu lie Asp Arg Gin Gly Arg lie Arg Leu Thr Met 675 680 685 aaa gac ate aat aat acc aac gag gca aat gca gaa gaa act gta get 2112

Lys Asp lie Asn Asn Thr Asn Glu Ala Asn Ala Glu Glu Thr Val Ala 690 695 700 gaa aat gtg gta gaa aca gaa caa gaa aat aat ttc ta 2150

Glu Asn Val Val Glu Thr Glu Gin Glu Asn Asn Phe 705 710 715 <210> 171 <211> 716 136852-序列表.doc -269- 1334884 <212>蛋白質 <213>涫血性巴斯埝样渎 <400> 171

Met Thr Pro lle Val Lys Gin Phe Lys Tyr Gly Gin His Thr Val Thr 1 5 10 15

Leu Glu Thr Gly Ala lie Ala Arg Gin Ala Thr Ala Ala Val Met Ala 20 25 30

Ser Met Asp Asp Thr Thr Val Phe Val Thr Val Val Ala Lys Lys Asp 35 4〇 45

Val Lys Glu Gly Gin Asp Phe Phe Pro Leu Thr Val Asp Tyr Gin Glu 50 55 60

Arg Thr Tyr Ala Ala Gly Arg lie Pro Gly Gly Phe Phe Lys Arg Glu 65 70 75 80

Gly Arg Pro Ser Glu Gly Glu Thr Leu lie Ala Arg Leu lie Asp Arg 65 90 95

Pro Val Arg Pro Leu Phe Pro Glu Gly Phe Phe Asn Glu lie Gin Val 100 105 110 lie Ala Thr Val Val Ser Val Asn Pro Gin lie Ser Pro Asp Leu Val 115 120 125

Ala Met lie Gly Ala Ser Ala Ala Leu Ser Leu Ser Gly Val Pro Phe 130 135 140

Asn Gly Pro lie Gly Ala Ala Arg Val Gly phe lie Asn Asp Gin Phe 145 150 155 160

Val Leu Asn Pro Thr Thr Ser Glu Gin Lys lie Ser Arg Leu Asp Leu 165 170 175

Val Val Ser Gly Thr Asp Lys Ala Val Leu Met Val Glu Ser Glu Ala 180 185 190

Asp lie Leu Thr Glu Glu Gin Met Leu Ala Ala Val Val Phe Gly His 195 200 205

Glu Gin Gin Gin Val Val lie Glu Asn lie Lys Glu Phe Val Lys Glu

210 215 220

Ala Gly Lvs Pro Arg Trp Asp Trp Val Ala Pro Glu Pro Asn Thr Asp 225 ' 230 235 240

Leu lie Asn Lys Val Lys Ala Leu Ala Glu Thr Arg Leu Gly Asp Ala 245 250 255

Tyr Arg lie Val Glu Lys Gin Val Arg Tyr Glu Gin lie Asp Ala lie 260 265 270

Lys Ala Glu Val He Ala Gin Leu Thr Ala Glu Asp Glu Thr Val Ser 275 280 285

Glu Gly Thr lie lie Asp lie lie Thr Ala Leu Glu Ser Gin lie Val 290 295 300

Arg Ser Arg lie lie Ala Gly Glu Pro Arg lie Asp Gly Arg Thr Val 305 310 315 " 320 •270· 136852·序列表.doc IZ1 1334884

Asp Thr Val Arg Ala Leu Asp lie Cys Thr Ser Val Leu Pro Arg Thr 325 330 335

His Gly Ser Ala Leu Phe Thr Arg Gly Glu Thr Gin Ala Leu Ala Val 340 345 350

Ala Thr Leu Gly Thr Glu Arg Asp Ala Gin lie lie Asp Glu Leu Thr 355 360 365

Gly Glu Lys Ser Asp Arg Phe Leu Phe His Tyr Asn Phe Pro Pro Tyr 370 375 360

Ser Val Gly Glu Thr Gly Arg lie Gly Ser Pro Lys Arg Arg Glu lie 385 390 395 400

Gly His Gly Arg Leu Ala Lys Arg Gly Val Leu Ala Val Met Pro Thr 405 410 415

Ala Glu Glu Phe Pro Tyr Val Val Arg Val Val Ser Glu lie Thr Glu 420 425 430

Ser Asn Gly Ser Ser Ser Met Ala Ser Val Cys Gly Ala Ser Leu Ala 435 440 445

Leu Met Asp Ala Gly Val Pro lie Lys Ala Ala Val Ala Gly He Ala 450 455 460

Met Gly Leu Val Lys Glu Asp Glu Lys Phe Val Val Leu Ser Asp lie 465 470 475 460

Leu Gly Asp Glu Asp His Leu Gly Asp Met Asp Phe Lys Val Ala Gly 485 490 495

Thr Arg Thr Gly Val Thr Ala Leu Gin Met Asp lie Lys lie Glu Gly 500 505 510 lie Thr Pro Glu lie Met Arg lie Ala Leu Asn Gin Ala Lys Gly Ala 515 520 525

Arg Met His lie Leu Gly Val Met Glu Gin Ala lie Pro Ala Pro Arg 530 535 540

Ala Asp lie Ser Asp Tyr Ala Pro Arg lie His Thr Met Lys lie Asp 545 550 555 560

Pro Lys Lys lie Lys Asp Val lie Gly Lys Gly Gly Ala Thr lie Arg 565 570 575

Ala Leu Thr Glu Glu Thr Asn Thr Ser lie Asp He Asp Asp Asp Gly 580 585 590

Thr Val Lys lie Ala Ala Thr Asp Gly Asn Ala Ala Lys Ala Val Met 595 600 605

Ala Arg lie Glu Glu lie Val Ala Glu Val Glu Val Asn Gin lie Tyr 610 615 620

Asn Gly Lys Val Thr Arg Val Val Asp Phe Gly Ala Phe Val Ser lie 625 630 635 640

Leu Gly Gly Lys Glu Gly Leu Val His lie Ser Gin lie Thr Asn Glu 645 650 655 136852-序列表.doc •271 ·

I 1334884

Arg Val Glu Arg Val Ala Asp Tyr Leu Thr Val Gly Gin Glu Val Gin 660 665 670

Val Lys Val Val Glu lie Asp Arg Gin Gly Arg lie Arg Leu Thr Met 675 680 6Θ5

Lys Asp lie Asn Asn Thr Asn Glu Ala Asn Ala Glu Glu Thr Val Ala 690 695 700

Glu Asn Val Val Glu Thr Glu Gin Glu Asn Asn Phe 705 710 715 <210> 172 <211> 1517 <212> DNA <213> ;容血n匕浙德#道 <220> <221> CDS <222> (1) . . (1515)

<220> <223> purF <400> 172 atg tgc ggc att gtc ggt att att ggg aat teg ccg gtg aat cag geg 48

Met Cys Gly lie Val Gly lie lie Gly Asn Ser Pro Val Asn Gin Ala 15 10 15 att tat gat ggt tta aca tta ett caa cac ega gga caa gat gcc gca 96 lie Tyr Asp Gly Leu Thr Leu Leu Gin His Arg Gly Gin Asp Ala Ala 20 25 30 ggt ate gtc acc ata gac gat gaa aat cgt ttc ege tta ege aaa get 144

Gly lie Val Thr lie Asp Asp Glu Asn Arg Phe Arg Leu Arg Lys Ala 35 40 45 aac ggc tta gtc age gat gtt ttc cag caa gag cat atg gtg aga tta 192

Asn Gly Leu Val Ser Asp Val Phe Gin Gin Glu His Met Val Arg beu 50 55 60 caa ggc aat gtt gga att ggt cac gtt ege tac cca aca gca ggt age 240

Gin Gly Asn Val Gly lie Gly His Val Arg Tyr Pro Thr Ala Gly Ser 65 70 75 80

tea agt gtg tet gaa gcc cag cca ttt tat gtc aat tea cct ttc ggt 2ΘΘ

Ser Ser Val Ser Glu Ala Gin Pro Phe Tyr Val Asn Ser Pro Phe Gly 85 90 95 att acc tta gtt cac aac ggt aat tta act aat aat geg gaa ett aaa 336 lie Thr Leu Val His Asn Gly Asn Leu Thr Asn Asn Ala Glu Leu Lys 100 105 110 get ege tta tac aac gaa gcc ege ege cat gtg aac act aat tet gat 384

Ala Arg Leu Tyr Asn Glu Ala Arg Arg His Val Asn Thr Asn Ser Asp 115 120 125 tet gaa tee ett ett aat att tet get tac ttt tta gat etc tat tee 432

Ser Glu Ser Leu Leu Asn lie Phe Ala Tyr Phe Leu Asp Leu Tyr Ser 130 135 140 act cag cat tea age cca gac aat ate ttt gaa aeg gtt cgt aaa acc 480 •272· 136852·序列表.doc

I SI 1334884

Thr 145 Gin His Leu Ser Pro 150 Asp Asn lie Phe Glu Thr 155 Val Arg Lys Thr 160 aat gat age att cgt ggt get tat get tgc att gcg atg att ate gga 528 Asn Asp Ser He Arg 165 Gly Ala Tyr Ala Cys 170 He Ala Met lie lie 175 Gly cac ggt atg gtt get ttc cgt gac cca ttc ggt att ege ccg tta gtg 576 His Gly Met Val 180 Ala Phe Arg Asp Pro 185 Phe Gly lie Arg Pro 190 Leu Val ctg ggt aaa cgt gaa ate gag ggt aaa acc gaa tat atg ttt get teg 624 Leu Gly Lys 195 Arg Glu lie Glu Gly 200 Lys Thr Glu Tyr Met 205 Phe Ala Ser gaa agt gtg get ett gat gta gtg ggg ttt gaa ttt gtg cga gat gtg 672 Glu Ser 210 Val Ala Leu Asp Val 215 Val Gly Phe Glu Phe 220 Val Arg Asp Val ctg ccg ggt gaa gcg att tat gtt acc ttt gat ggg caa tta cat teg 720 Leu 225 Pro Gly Glu Ala lie 230 Tyr Val Thr Phe Asp Gly Gin 235 Leu His Ser 240 caa att tgt gcc gat aat cca aaa ctg aat cct tgt att lie ttt gaa tat 768 Gin lie Cys Ala Asp 245 Asn Pro Lys Leu Asn 250 Pro Cys Phe Glu Tyr 255 gtt tat ttt gcc cgt cct gat tcc gtc att gat ggc gtt tct gta tat 816 Val Tyr Phe Ala 260 Arg Pro Asp Ser Val 265 lie Asp Gly Val Ser 270 Val Tyr tct gca cga gtg cat atg ggc gaa tta tta ggt gag aaa att aaa cgt 864 Ser Ala Arg 275 Val His Met Gly Glu 280 Leu Leu Gly Glu Lys 285 lie Lys Arg gaa tgg gga cga att ate gat gat att gat gtg gtg ate ccg att cct 912 Glu Trp 290 Gly Arg lie lie Asp 295 Asp lie Asp Val Val 300 lie Pro lie Pro gaa acc tea aat gat att gcg gta cgt att get aat atg ttg tat aaa 960 Glu 305 Thr Ser Asn Asp lie 310 Ala Val Arg lie Ala Asn 315 Met Leu Tyr Lys 320 ccc tat cgt caa ggg ttt gtt aaa aac ege tat gta get cga act ttt 1008 Pro Tyr Arg Gin Gly 325 Phe Val Lys Asn Arg 330 Tyr Val Ala Arg Thr 335 Phe att atg ccg ggg caa gca cag cgt aaa age teg gtt ege cgt aaa tta 1056 lie Met Pro Gly 340 Gin Ala Gin Arg Lys 345 Ser Ser Val Arg Arg 350 Lys Leu aat gcg att gcc tct gaa ttt aaa ggc aaa age gtg tta ctg gtt gat 1104 Asn Ala lie 355 Ala Ser Glu Phe Lys 360 Gly Lys Ser Val Leu 365 Leu Val Asp gat tct att gta cga ggt aca a eg tct gaa caa ate gtg gaa atg gca 1152 Asp Ser 370 lie Val Arg Gly Thr 375 Thr Ser Glu Gin lie 380 Val Glu Met Ala cga gca get ggt gca aaa egg gtt tat ttt gcc tct gcc gca ccg gaa 1200 Arg 385 Ala Ala Gly Ala Lys 390 Arg Val Tyr Phe Ala Ser 395 Ala Ala Pro Glu 400 Π6852·序歹!1 表.doc •273

i SI 1334884

att lie cgc Arg tac Tyr ccg Pro aat Asn 405 gtg Val tat ggc Tyr Gly att He gat Asp 410 atg Met ccg Pro act Thr tgt Cys gaa Glu 415 gaa Glu 1248 tta Leu gtg Val get Ala tat Tyr 420 gat Asp cgc Arg tea Ser gtg Val gaa Glu 425 gag Glu gtt Val gca Ala cag Gin atg Met 430 ata lie ggg Gly 1296 gtg Val gat Asp aaa Lys 435 ttg Leu att lie ttc Phe caa Jin gac Asp 440 ett Leu gaa Glu gca Ala ett Leu tat Tyr 445 aag Lys tet ser att lie 1344 caa Gin ctg Leu 450 gaa Glu aat Asn ccg Pro act Thr att lie 455 cat His cgc Arg ttt Phe gat gac Asp Asp 460 tet Ser gta Val ttt Phe aca Thr 1392 gga gaa Gly Glu 465 tat Tyr att lie aca Thr ggt gat Gly Asp 470 gta Val gat aaa Asp Lys tgc Cys 475 tat Tyr tta Leu gac Asp agt Ser ata lie 480 1440 gca Ala aga Arg tet Ser ega Arg aac gat Asn Asp 485 aaa Lys gca Ala aaa Lys gca Ala 490 gag Glu geg Ala gca Ala aaa Lys caa Gin 495 gec Ala I486 acc Thr aat Asn tta Leu gaa Glu 500 att lie cat His aac Asn gaa Glu aga Arg 505 ta 1517 <210> 173 <211> 505 <212>蛋白質 <213 >溶血性巴斯德桴菌 <400> 173 Met Cys Gly 1 lie Val 5 Gly lie lie Gly Asn 10 Ser Pro Val Asn Gin 15 Ala lie Tyr Asp Gly 20 Leu Thr Leu Leu Gin 25 His Arg Gly Gin Asp 30 Ala Ala Gly lie Val 35 Thr lie Asp Asp Glu 40 Asn Arg Phe Arg Leu 45 Arg Lys Ala Asn Gly 50 Leu Val Ser Asp Val 55 Phe Gin Gin Glu His 60 Met Val Arg Leu Gin 65 Gly Asn Val Gly lie 70 Gly His Val Arg Tvr 75 Pro Thr Ala Gly Ser 80

Ser Ser Val Ser Glu Ala Gin Pro Phe Tyr Val Asn Ser Pro Phe Gly 85 90 95 lie Thr Leu Val His Asn Gly Asn Leu Thr Asn Asn Ala Glu Leu Lys 100 105 110

Ala Arg Leu Tyr Asn Glu Ala Arg Arg His Val Asn Thr Asn Ser Asp 115 120 125

Ser Glu Ser Leu Leu Asn lie Phe Ala Tyr Phe Leu Asp Leu Tyr Ser 130 135 140 .274· 136852-序列表.doc i si 1334884

Thr Gin His Leu Ser Pro Asp Asn lie Phe Glu Thr Val Arg Lys Thr 145 150 155 160

Asn Asp Ser lie Arg Gly Ala Tyr Ala Cys He Ala Met lie lie Gly 165 170 175

His Gly Met Val Ala Phe Arg Asp Pro Phe Gly lie Arg Pro Leu Val 180 185 190

Leu Gly Lys Arg Glu lie Glu G^y Lys Thr Glu Tyr Met Phe Ala Ser 195 200 205

Glu Ser Val Ala Leu Asp Val Val Gly Phe Glu Phe Val Arg Asp Val 210 215 220

Leu Pro Gly Glu Ala lie Tyr Val Thr Phe Asp Gly Gin Leu His Ser 225 230 235 240

Gin lie Cys Ala Asp Asn Pro Lys Leu Asn Pro Cys lie Phe Glu Tyr 245 250 255

Val Tyr Phe Ala Arg Pro Asp Ser Val lie Asp Gly Val Ser Val Tyr 260 265 270

Ser Ala Arg Val His Met Gly Glu Leu Leu Gly Glu Lys lie Lys Aig 275 280 285

Glu Trp Gly Arg lie lie Asp Asp lie Asp Val Val lie Pro lie Pro 290 295 300

Glu Thr Ser Asn Asp lie Ala Val Arg lie Ala Asn Met Leu Tyr Lys 305 310 315 320

Pro Tyr Arg Gin Gly Phe Val Lys Asn Arg Tyr Val Ala Arg Thr Phe 325 330 335

He Met Pro Gly Gin Ala Gin Arg Lys Ser Ser Val Arg Arg Lys Leu 340 345 350

Asn Ala lie Ala Ser Glu Phe Lys Gly Lys Ser Val Leu Leu Val Asp 355 360 365

Asp Ser lie Val Arg Gly Thr Thr Ser Glu Gin He Val Glu Met Ala 370 375 380

Arg Ala Ala Gly Ala Lys Arg Val Tyr Phe Ala Ser Ala Ala Pro Glu 385 390 395 400

He Arg Tyr Pro Asn Val Tyr Gly lie Asp Met Pro Thr Cys Glu Glu 405 410 415

Leu Val Ala Tyr Asp Arg Ser Val Glu Glu Val Ala Gin Met He Gly 420 425 430

Val Asp Lys Leu lie Phe Gin Asp Leu Glu Ala Leu Tyr Lys Ser lie 435 440 445

Gin Leu Glu Asn Pro Thr lie His Arg Phe Asp Asp Ser Val Phe Thr 450 455 460

Gly Glu Tyr lie Thr Gly Asp Val Asp Lys Cys Tyr Leu Asp Ser lie 465 470 475 480 -275- 136852-序列表.doc i si 1334884

Ala Arg Ser Arg Asn Asp Lys Ala Lys Ala Glu Ala Ala Lys Gin Ala 485 490 495

Thr Asn Leu Glu He His Asn Glu Arg 500 505

<210> 174 <211> 386 、212> DNA <213 > /容血性巴斯德桿描 <220> <221> CDS <222> (1)..(384) <220> <223> yjgF <400> 174 atg aca gtt ate cac aca gaa aat gca ccg gca geg att ggg cct tat 48

Met Thr Val lie His Thr Glu Asn Ala Pro Ala Ala lie Gly Pro Tyr

15 10 15 gtg caa gca gtt gat tta ggc aat atg gtt tta act tet ggg caa att 96

Val Gin Ala Val Asp Leu Gly Asn Met Val Leu Thr Ser Gly Gin lie 20 25 30 ccc gtg aat cct gaa acc ggc gaa ate ccg agt gat att gtg caa caa 144

Pro Val Asn Pro Glu Thr Gly Glu lie Pro Ser Asp lie Val Gin Gin 35 40 45 acc ege caa tet ctg aac aac gtg aaa gcc att ate gaa caa gee ggc 192

Thr Arg Gin Ser Leu Asn Asn Val Lys Ala lie lie Glu Gin Ala Gly 50 55 60 tta acc gtt gcc gat att gta aag acc acc gta ttt gtc aaa gat ett 240

Leu Thr Val Ala Asp He Val bys Thr Thr Val Phe Val Lys Asp Leu 65 70 75 8〇 aac gac ttc gca aag gta aat geg gaa tac caa gcc ttc ttc caa gaa 288

Asn Asp Phe Ala Lys Val Asn Ala Glu Tyr Gin Ala Phe Phe Gin Glu 85 90 95

aac gaa cac cct aat ttt ccg get cgt tet tgc gta gaa gtg get cgt 336

Asn Glu His Pro Asn Phe Pro Ala Arg Ser Cys Val Glu Val Ala Arg

100 105 HO tta cca aaa gat gtt ggc att gag ate gaa geg att gca gta ege ega 384

Leu Pro Lys Asp Val Gly He Glu He Glu Ala He Ala Val Arg Arg 115 120 125 <210> 175 <211> 128 <212>蛋白筲 <213〉溶血性巴斯德桴菌 <400> 175

Met Thr Val lie His Thr Glu Asn Ala Pro Ala Ala lie Gly Pro Tyr 15 l〇 15 136852-序列表.doc -276-

1 SI 1334884

Val Gin Ala Val Asp Leu Gly Asn Met Val Leu Thr Ser Gly Gin He 20 25 30

Pro Val Asn Pro Glu Thr Gly Glu He Pro Ser Asp He Val Gin Gin 35 40 45

Thr Arg Gin Ser Leu Asn Asn Val Lys Ala lie lie Glu Gin Ala Gly 50 55 60

Leu Thr Val Ala Asp lie Val Lys Thr Thr Vax Phe Val Lys Asp Leu 65 70 75 80

Asn Asp Phe Ala Lys Val Asn Ala Glu Tyr Gin Ala Phe Phe Gin Glu 85 90 95

Asn Glu His Pro Asn Phe Pro Ala Arg Ser Cys Val Glu Val Ala Arg 100 105 110

Leu Pro Lys Asp Val Gly lie Glu lie Glu Ala lie Ala Val Arg Arg 115 120 125

<210> 176 <21l>20 <2I2> DNA <2 Π>人造的序蚵 <220> <221> <222> <227>人造序列的說明：引子 <40(i> 176 alggcnggng cntuirgiiral <2I0> 177 <2l!>2〇 <212> DNA <2丨3>人造的外叫 <22t)> <221> <222> <223>人造序列的說明：引子 <22()>

<221> misc <222> 3 " <22>»=八或|或〇或1 <22()> <221> misc 特徵 <222> 12 <223>n=A或丨或或e <22(i> <22!>niisc <222>15 <223>η=Λ 或Τ或 <40ίί> 177 gcngcvltca ingcnacuai <210> Ι7Κ • 277· 136852-序列表.doc 1334884 <211>21 <2I2> DNA <2 Π>人造的序列 <220> <223>人造序列的說明：引户 <22〇> <22\> <222> 3 ' <223> N=A或T或(i 或C <400> 178 ggnUyulyc iiyaaiiiuiviit g 21

<21〇> I7l； <2II>23 <2Ι2> Ι)ΝΛ <:2丨> 人迄的序和； <22〇> <223>人迳序列的說明：引子<22(，> <22丨> 丨11丨$。_特徵 <222> 6 ' <223> Ν=Λ或Τ或(i或C’ <220> <22l>misejf寺徵 <222> 12 <223〉Ν=Λ4Τ或U 或C <220> <22丨〉misc_特徵 <222>15 <223> N=A或T或G或C <400> 179 Icttlngtra tngtnucutc rig <210> IS(» <2\\>24 <2\2> DNA <2Π>人造的序列 <220> <223>人這序列的說明：引子 <400> 180 gcsggyaiuic ttgg <210> IS! <2il>25 <2Ι2>Ι)ΝΛ <2丨3>人造的序列 <22〇> <223>人造序列的說明：引子 <>1X1 crccUiiiriit rlclgiumgc ciccac 23 24 25 136852·序列表.doc 278- τ -r· λ ί a i 1334884

<2H)> 1X2 <21 l>20 <2I2> Ι)ΝΛ <21>人.造的序和； <22〇> <223>人造序列的說明：引子 <220> <22l>n)isc_iiHl <222> 9 ~ <223> Ν=Λ或T或(;或C -*22〇> <22 I > inisc_4i ί^. <222>Ι5 <223> Ν=Λ或Ί’或(;或C <22()> <22l>miscjm <222>1S 或T 或(ί 或 C <4(Η)> !Χ2 <2Ι0> ΙΧλ <21Ι> 21 <212> Ι)ΝΛ <2丨3>人造的序和； <22()> <223>人迻序列的說明引子 <4()0>183 calcilcauUj ccuUicacat t <2l〇> IS4 <21]> 16 <212〉Ι)ΝΛ <2丨3>人造的序列 <22〇> ，223>人造序列的說明：引子 <22〇> <22 丨 > 徵 <222> 3 ' Ν=ΛΙΓ成U 或t_ <221>misc_i!Hl <222> f) ~ <223> Ν=Λ或丨或(;或C <220> <22丨> 111丨81；_特徵 <222> 12 <22>?^=六或'丨’或(;或C <41H)> 1S-4 i：»ncciUii>g incurs 20 2) 16 136852-序列表.doc -279- 1 si 1334884

<2i()> 1K5 <2II> 15 <2I2> Ι)ΝΛ <2Π>人造的序夕·： <22〇> <22弘人迮汴^；的汉叫：W r <22〇> <22\> <222> !" <223>Ν=Λ或丨或C;或(’ <22〇> <22 丨 > <222> 4 ~ ·:22> Ν=Λ或丨Ό 或C· <22〇> ‘·22卜丨丨丨丨从._纣沆 <-222> 10 <223> Ν=Λ或丨或〔i或C <40()> 185 ngcniityicn iicrcu <2Π)> ixr> <2II>22 <2I2> 1)ΝΛ <ΪΠ：>々造的序A: <22〇> <223>人造序K的說明：引子 <-4()(ϊ> IS(i giiiigccgccii UJCgfUllg ΰμ <2IU：> 1X7 <2I2> Ι)ΝΛ 、:2丨> 人边的 <22〇> <223>人造序叫的說明：W子 <-4()t)> 187 iittycltccl UycctLiciu: [*2ii <210> ISS <2II>24 <2I2> 1>ΝΛ <2丨3>人造的序列 <22U> <22>人造斤《|;的汉叫：W r <400-- iSS 畔k·叫ί“ΙίΙ CiUtUlCCilCt Ucu IS9 <21 l> 21 <2I2> DNA <2丨3>人造的序列 15 22 23 24 136852-序列表.doc -280· is】 211334884

<22i)> <223>人造序列的說明：引千 <41)(》> 1X9 geaecaaii^L· Ltgaattt^tc c <2I0> 1^) -21i> 2A <2I2> Ι)ΝΛ <2丨3>人造的序列 <220> <223>人造序列的說明：引子 <400> 190 ggtgatgatji Icgatgiila» lax <21ίί> 191 <2\)>1Α <Μ2:、Ι)ΝΛ -2Π:、人it的/Μ;; <22〇> <22 >人造序列的說明引子 <4()()> 191 ggcglullag ccglgiitiiLX· aacc <2I0> Ιι；2 <2ii>23 12> I >ΝΛ <?丨> 人边的 <220> <223>人造序列的說明：引子 <400> 11；2 gilCCilCtlil" gc»illatugil ctl <2I0> 1^3 .*21 |> 22 、212、I>NA 、:2丨3>人造的序A; <22〇> <223>人造序列的說明：41子 <4U()> 19.-¾ cicailcatiUJ iilc»cdu;U tc <21〇> 1^-4 <2ΙΙ>2〇 <2Ι2> Ι)ΝΛ <2丨>人造的>ί·β <22〇> <223>人造序列的說明：^丨子 <400> W4 JlCCtgCUgCiJ icltglcclc <2I0> 195 <21]> 20 24 24 23 22 20 136852-序列表.doc -281 - 201334884 <2Ι2> Ι)ΝΛ 人边的序β <220> c223>人造>f·列的說明：W十 <^0(ϊ> Ιι)5 ilC^ggllUil lltgcctctg <2!(ϊ> IVi) (:211> 22 •·2Ι2> Ι)ΝΛ 人造的卟和； <22(ϊ> <223>人造序列的說明：引子 <40U> !% 22 cgccg»tttc aggattcacg

<21ίί> \ ΧΠ <211> 20 *212- · DNA 」13>人迮的序β <220> <223>人造外列的說明：引子 <4()(»> 1^7 clgiunaHCii lgci;umcc；u 20

J36852-序列表.doc -282-

1 SI

Claims

34884

第0971^48939號專利申請案中文申睛專利範圍替換本(99年10月）十、申請專利範圍： 1' 一種減毒巴斯德桿菌（户ajiewreZ/α)細菌，其包含一基因中之—突變，該基因係以SEQ ID NO:76之核誓酸序列所表示’或巴斯德桿菌之物種同系物，其中該物種同系物與SEQ iD N〇:76之核苷酸序列具有9〇%或更高之序列相似性’該突變導致由該突變基因所編碼之基因產物的活性降低。 2.如請求項1之巴斯德細菌，其中該突變導致由該突變基因所編碼之基因產物的表現降低。 3·如請求項1之巴斯德細菌’其中該突變導致由該突變基因所編碼之失活基因產物的表現。 4·如請求項1之巴斯德細菌’其中該突變導致該基因的全部或一部份被刪除。 5'如請求項1之巴斯德細菌，其中該巴斯德細菌係敗血性巴斯德桿菌mw/iocWa)。 6_如凊求項5之巴斯德細菌，其中該突變導致由該突變基因所編碼之基因產物的表現降低。 7.如凊求項5之巴斯德細菌，其中該突變導致由該突變基因所編碼之失活基因產物的表現。 8如°月求項5之巴斯德細菌’其中該突變導致該基因的全部或—部份被刪除。種免疫原組合物，其包括請求項丨至8中任一項的細菌。 1〇種疫邊組合物，其包括請求項9之免疫原組合物和在 136852-991008.doc 1334884 藥學上可接受的載劑。 11. 12. 月长項10之疫忐組合物，其進一步包括佐劑。一種產製革蘭氏陰性細菌突變種的方法，包括在基因中' 導入突變的步驟，該基因係以SEQID NO:76之核答酸序列所表示，或其物種同系物，其中該物種同系物與SEQ ID NO:76之核菩酸序列具有9〇%或更高之序列相似性，該突變導致由該突變基因所編碼之基因產物的活性降低。 13. 一種產製減毒之巴斯德細菌的方法，其包括在基因中導 _ 入突變的步驟，該基因係以SEQ ID N〇:76之核苷酸序列所表不’或其物種同系物’其中該物種同系物與sEq ID NO:76之核苷酸序列具有9〇%或更高之序列相似性，該突變導致由該突變基因所編碼之基因產物的活性降低。 14. 15. 一種經過純化和分離的巴斯德桿菌多核甞酸，其包括一 SEQ ID NO:76所示之核：y：酸序列。一種編碼巴斯德桿菌毒力基因產物或其物種同系物之經過純化和分離之多核站酸’其中該多核苷酸係選自由下 ® 述所組成之群組： a) 編碼由包含SEq id NO:76之核苷酸序列所編碼之多肽的多核苷酸，及 b) 在高等嚴格的條件下，與（a)之多核苷酸的互補物雜交的多核苷酸；且其中該物種同系物與SEQ ID NO:76之核省:酸序列具有90%或更高之序列相似性。 136852-991008.doc 1334884 16 · —種經過純化和分離之巴斯德桿菌多核芬酸，其編碼且有SEQ ID NO:77之胺基酸序列的多肽。 17. 如請求項16之多核苷酸，其為DNA。 18. —種載體，其包括請求項17之DNA。 19. 如請求項18之載體，其為表現載體，其中該dn A以可操作之方式與表現控制DNA序列連接。

20. —種以請求項17之DNA穩定地轉化或轉移感染的宿主細胞，其係容許該編碼多肽在該宿主細胞中表現。 21_ —種產製重組多肽的方法，其包括使請求項2〇之宿主細胞生長在營養培養基中’並從該宿主細胞或該營養培養基中分離該編碼多肤。 22· —種藉著請求項21之方法產製的經過純化之多肽。 23· —種經過純化之多肽’其包括具有SEq ο no : 77之胺基酸序列之多肽。 24· 一種抗體，其係專一地與請求項23之多肽產生反應。 25.如請求項24之抗體，其為單株抗體。 136852.991008.doc