TW201709810A - Pogostemon cablin cultivation method having a large-sized leaf in average and a high content and concentration of carvacrol - Google Patents

Abstract

A pogostemon cablin cultivation method comprises the steps of: (A) providing pogostemon cablin with light illumination for at least eight hours on a daily basis, in which the light illumination is a kind of light ray illumination providing wavelength of 440nm to 480nm and 620nm to 660nm; and (B) soaking the pogostemon cablin in a liquid fertilizer for eight hours to twelve hours while providing the pogostemon cablin with the light illumination, in which the liquid fertilizer comprises nitrogen with a concentration of 100ppm to 243.75ppm, phosphorus with a concentration of 100ppm to 200ppm, and potassium with a concentration of 100ppm to 200ppm. Accordingly, with the disclosed pogostemon cablin cultivation method, the cultivated pogostemon cablin can have a large-sized leaf in average and a high content and concentration of carvacrol, i.e., an excessive concentration is not required.

Description

Left hand incense cultivation method

The invention relates to a plant cultivation method, in particular to a left-handed fragrance cultivation method.

The left-handed scent is a perennial herb that is a widely used and medicinal plant. The efficacy of left hand fragrance, including antibacterial and anti-inflammatory. The active ingredients contained in the left-handed scent are probably δ-3-Carene (16.3%), Carvacrol (13.4%), Camphor (12.3%), γ-Terpinene (11.9%), α-Terpinene (6.9%), ρ- Cymene (6.1%), β-Caryophyllene (3.5%), α-Bergamotene (3.3%), Limonene (2.2%), and Thymol (0.06%). Among them, carvacrol is the most important.

Carvacrol has a pungent scent and is commonly used as an antibacterial, antiseptic and bactericide. Since carvacrol has the aforementioned characteristics and can be obtained from the left-handed fragrance, safety is considered to be high relative to chemically synthesized agents, and thus it has been added to many health care products such as mouthwashes and toothpastes and the like. Although the left-handed extract has an antibacterial effect because it has carvacrol, it is necessary to add a considerable amount of the left-handed extract to the health care product in order to achieve a better antibacterial effect. When the left-handed extract is added too much, it will be squeezed into the amount of other ingredients and cause problems. For example, if a toothpaste is added with too much left-handed extract, it cannot be used as a paste and is not convenient to use. In order to solve This left-handed extract has troubles. It is a feasible method to repeatedly concentrate the left-handed extract. It is just too much concentration method. In addition to destroying the active ingredients in the extract, it will greatly increase the production cost and the imbalance between profit and loss.

The object of the present invention is to provide a method for cultivating a left-handed scent, which can be added to a health care product without the need for complicated concentration treatment after being extracted.

The method of cultivating the left-handed scent comprises: step A providing a left-handed scent for at least 8 hours of illumination in a day, the illumination being provided by irradiation of light of one of wavelengths of 440 nm to 480 nm and 620 nm to 660 nm. Step B: Soak the left hand fragrance in the liquid fertilizer for 8 hours to 12 hours while providing the light. The liquid fertilizer includes nitrogen at a concentration of 100 ppm to 243.75 ppm, phosphorus at 100 ppm to 200 ppm, and potassium at 100 ppm to 200 ppm.

The effect of the left-handed incubating method is to provide a specific time and a specific wavelength of light and the liquid fertilizer, and to make the liquid fertilizer have a specific concentration of nutrients, and the average leaf diameter of the left-handed incense cultivated is larger, except The total content of carvacrol is higher, and the total concentration is also higher. The prepared left-handed extract can be directly added to health care products without excessive concentration.

Other features and effects of the present invention will be apparent from the embodiments of the drawings, wherein: 1 is a graph showing the relationship between the illumination wavelength and the average leaf diameter in Examples 33 to 36 and Comparative Examples 1 to 5 of the left-handed incense cultivation method of the present invention; FIG. 2 is another discussion of illumination of Examples 33 to 36 and Comparative Example 4. A plot of wavelength versus mean leaf diameter.

Before the embodiments are described in detail, it should be noted that in the following description, similar elements are denoted by the same reference numerals.

"Embodiment 1"

Example 1 of the method for cultivating the left-handed scent of the present invention is a left-handed scent applied to hydroponic cultivation. 8 hours of light and liquid fertilizer are provided for the left hand fragrance at the same time. The illumination is such that the LED produces a light with a specific wavelength of 660 nm to illuminate the left hand fragrance. The liquid fertilizer contained nitrogen and phosphorus at a concentration of 100 ppm, respectively, and potassium at a concentration of 200 ppm. After a total of 28 days of breeding under the aforementioned conditions, an average leaf diameter of 3 cm left-handed scent can be cultivated. The average leaf diameter is any 10 leaves, and the maximum width is measured by a ruler and averaged to obtain the average leaf diameter.

Providing liquid fertilizer means immersing the root of the left-handed scent in liquid fertilizer. When the left-handed fragrance is not immersed in liquid fertilizer, it is cultured by hydroponic method. The advantage of using hydroponic farming is that it is easy to switch between aquaculture water and liquid fertilizer, but not limited to hydroponic farming.

Embodiments 2 to 32

Examples 2 to 32 are similar to the embodiment 1, except that the light provided by each embodiment is different from the time of the liquid fertilizer, and the liquid fertilizer used contains different concentrations of nitrogen, phosphorus and potassium. Implementation The number of hours of illumination and liquid fertilizer supplied, the concentration of nutrients contained in the liquid fertilizer, and the average leaf diameter of the leaves of the cultivated left-handed scent were recorded in Table 1, respectively.

If the time of providing light is inconsistent with the time of providing liquid fertilizer, it will start providing light and provide liquid fertilizer at the same time, and at the end of one time, the other will continue. In the description of Example 2, after providing light and liquid fertilizer for 8 hours, the supply of light was stopped, and the liquid fertilizer was further supplied for 4 hours. In the description of Example 10, when the light and liquid fertilizer were simultaneously supplied for 8 hours, the supply of liquid fertilizer was stopped and the illumination was continued for 4 hours. In actual implementation, it is not necessary to start at the same time. For example, in the second embodiment, after providing 2 hours of liquid fertilizer, 8 hours of light and liquid fertilizer are provided, and after providing illumination, 2 hours is provided. The liquid manure, which is completed for a total of 8 hours, provides the time for the liquid to be supplied, with 12 hours of liquid fertilizer.

"Examples 33 to 36, Comparative Examples 1 to 5"

Examples 33 to 36, and Comparative Examples 1 to 5, were respectively provided with left-handed fragrance for 16 hours of daily light, and while left-handed, the left-handed fragrance was immersed in the liquid fertilizer for 12 hours while providing light. The culture was carried out for a total of 28 days under the aforementioned conditions. The illumination is irradiated with left-handed scent by light having a wavelength of 400 nm to 950 nm, respectively. The liquid fertilizer includes 200 ppm of nitrogen, 200 ppm of phosphorus, and 200 ppm of potassium. The exact wavelengths used in the respective examples and comparative examples, as well as the average leaf diameter of the cultivated left-handed scent, are shown in Table 3.

Referring to Examples 1 to 32, it can be found that when the light is provided The exposure time is 8 hours to 17.5 hours, the liquid fertilizer is supplied for 8 hours to 12 hours, the liquid fertilizer contains nitrogen concentration of 100 ppm to 243.75 ppm, and the liquid fertilizer contains phosphorus and potassium concentrations, respectively. When it is 100 ppm to 200 ppm, the leaves of the left-handed scent that are cultured can have an average leaf diameter of more than 3 cm.

Further analysis revealed that when the time of illumination was less than 10 hours, but the concentration of nitrogen contained in the liquid fertilizer was above 200 ppm, as in Examples 5 to 8, the average leaf diameter of the leaves could be further increased to more than 4 cm. If the illumination time is further increased to more than 12 hours, as in Examples 10 to 12, Example 14, Examples 16 to 17, and Examples 19 to 32, the leaves of the left-handed scent are cultivated regardless of the concentration of the liquid fertilizer. The average leaf diameter can be increased to more than 5 cm.

Observing Examples 16 to 17, and Examples 19 to 21, it was found that when the time for providing light and liquid fertilizer was more than 12 hours, and the concentration of the liquid fertilizer was 175 ppm or more, the leaves of the left-handed scent were cultivated. The average leaf diameter can be increased to more than 6 cm.

Observation of Examples 23 to 32 revealed that when the time for providing light was increased to 13 hours or more, the average leaf diameter of the leaves of the cultivated left-handed scent could be increased to 8 cm or more. If the time for providing light is further increased to 14.5 hours or longer, the width of the leaves of the cultivated left-handed incense can be increased to 9 cm or more, for example, Examples 26 to 32. Finally, when the time to provide light is increased to more than 16 hours, the width of the leaves of the cultivated left-handed incense can be increased to more than 10 cm, as in Examples 29 to 32. It is worth noting that when the illumination time exceeds 16 hours, it can no longer be effectively The average leaf diameter of the leaves of the left-handed scent is increased.

Next, the concentrations of carvacrol which the leaves of the left-handed scent which were cultivated were analyzed for the wild left-handed scent, and Examples 1, 10, 21, 25, and 29 were used. The analysis method was as follows: 5 g of the left-handed leaves of each group of experiments were taken, and 45 g of water was added thereto, and 2.5 g of the solution was taken to determine the concentration of carvacrol, and the test results were recorded in Table 2. As can be seen from Table 2, the wild left hand scent which was not cultivated by the method of the present invention had an average leaf diameter of 1 cm and a carophenol concentration of 5 ppm. The left-handed scent cultivated by the method of the present invention has an average leaf diameter of about 3 to 10 cm and a concentration of 20 ppm to 225 ppm.

Further comparisons revealed that when the average leaf diameter was increased from 1 cm to 3 cm, the concentration of carvacrol was 4 times that of wild left-handed scent. When the average leaf diameter was increased to 5 cm, the concentration of carvacrol was 24 times that of the wild left-handed scent, which was 6 times that of the left-handed scent with an average leaf diameter of 3 cm. When the average leaf diameter increased by about 7 cm, the concentration of carvacrol was 36 times that of the wild left-handed scent, which was 1.5 times that of the left-handed scent with an average leaf diameter of 5 cm. When the average leaf diameter was increased to about 9 cm, the concentration of carvacrol was 40 times that of the wild left-handed scent, which was 1.11 times that of the left-handed scent with an average leaf diameter of about 7 cm. When the average leaf diameter was increased to 10 cm, the concentration of carvacrol was 45 times that of wild left-handed scent, which was 1.13 times that of the left-handed scent with an average leaf diameter of about 9 cm. The concentration of carvacrol in the left-handed scent cultivated in this case is much higher than that of the wild-type left-handed scent of carvacrol, which is quite amazing.

The meaning of the column E in Table 2 is the multiple of the concentration of carvacrol that can be increased per unit of illumination time. We can find that when lighting At the beginning of the increase, the multiple of the concentration of carvacrol which can be increased per unit of illumination time is increased from 0.5 times to 3 times. At the same time, we can also find that when the illumination time is increased to more than 12 hours, the extended illumination time can also increase the concentration of carvacrol, but the efficiency is slightly reduced from 3 times to 2.81 times. Therefore, it can be inferred that with efficiency as a priority, providing 12 hours of light per day is the best breeding method.

It should be noted that the growth of the left-handed incense is a continuous process. When the amount of light and liquid fertilizer supplied is longer, or the concentration of each nutrient component of the liquid fertilizer is higher, the cultivated left-handed scent naturally has a larger average leaf diameter in a shorter culture time. Conversely, if the time provided for the light and liquid fertilizer is short, or when the concentration of each nutrient component of the liquid fertilizer is low, the cultivated left-handed scent may have a larger average leaf diameter at a longer culture time. Therefore, suitable incubation times can be adjusted slightly, preferably between 21 and 35 days. When it is less than 21 days, the left hand fragrance cultivated cannot have a larger average leaf diameter. When it is more than 35 days, the average leaf diameter growth of the cultivated left-handed scent is limited, but it increases the cost.

Referring to Fig. 1, Fig. 2 and Table 3, it can be observed that in Examples 33 to 36 and Comparative Examples 1 to 5, under the other conditions, the left hand fragrance is irradiated with light having a wavelength of 660 nm, and the left hand with the largest average leaf diameter can be cultivated. Fragrant, its average leaf diameter can reach 10.1 cm. The left-handed scent is irradiated with light having a wavelength of 440 nm and 620 nm, and the left-handed scent having the second largest average leaf diameter can be cultivated, and the average leaf diameter can reach 7.5 cm. Using light with a wavelength of 480 nm, the left-handed scent with the third largest average leaf diameter can be produced, and the average leaf diameter can reach 6.1 cm.

It is particularly noteworthy that the wavelength of the light is between 440 nm and 660 nm, and the relationship between the average leaf diameter and the illumination wavelength exhibits a U-shaped curve relationship as shown in FIG. 2, that is, in the visible light range, the effect is excellent, then decremented and again. increase. The inventor of the present invention found that the wavelength of the left-handed scent can be beautiful, but the average leaf diameter cannot be improved, and it is unexpectedly found that not all visible light contributes to the growth of the left-handed scent. LED LEDs produce light of a specific wavelength to optimize the cultivation of left-handed scent.

In summary, the efficacy of the method for cultivating the left-handed scent of the present invention is to provide a large average average leaf diameter and a total amount of carvacrol contained in the left-handed scent produced by the illumination of the specific hours and wavelengths and the liquid fertilizer. It is high, no need to repeat concentration, the concentration can be increased to 4 to 45 times of the original, has better bactericidal effect and can be directly added to the value of health care products, will not affect the quality of health care products, and achieve this The purpose of the invention.

However, the above is only the embodiment of the present invention, and the scope of the invention is not limited thereto, and all the simple equivalent changes and modifications according to the scope of the patent application and the patent specification of the present invention are still Within the scope of the invention patent.

Claims (8)

A method for cultivating a left-handed scent comprising: step A: providing a left-handed scent for at least 8 hours of light in a day, the illumination being provided by light irradiation of one of wavelengths of 440 nm to 480 nm and 620 nm to 660 nm; step B: While providing light, the left hand fragrance is immersed in the liquid fertilizer for 8 hours to 12 hours, and the liquid fertilizer includes nitrogen at a concentration of 100 ppm to 243.75 ppm, phosphorus at 100 ppm to 200 ppm, and potassium at 100 ppm to 200 ppm. The left-handed incense cultivation method according to claim 1, wherein the cultivation is carried out for 21 days to 35 days under the aforementioned conditions. The left-handed incense cultivation method according to claim 2, wherein the liquid fertilizer has a nitrogen concentration of 200 ppm or more when the time for providing the light in the step A is less than 10 hours. The left-handed incense cultivation method according to claim 2, wherein in the step A, the time for providing the light is from 12 hours to 17.5 hours. The left-handed incense cultivation method according to claim 4, wherein the liquid fertilizer is supplied in the step B for 12 hours, and the liquid fertilizer has a nitrogen concentration of 175 ppm or more. The left-hand cultivation method according to claim 5, wherein in the step A, the illumination is provided from 13 hours to 17.5 hours. The left-hand cultivation method according to claim 6, wherein in the step A, the illumination is provided at 14.5 hours to 17.5 hours. The left-handed incense cultivation method according to claim 7, wherein in the step A In the middle, the time for providing light is 16 hours.