TW201642882A - Nutrient tonic containing bee larvae - Google Patents

Abstract

Disclosed are a restorative tonic including bee larvae, a sexual-function-improving agent including bee larvae, a male-menopausal-symptom-improving agent including bee larvae, and an anti-fatigue agent including bee larvae.

Description

Nourishing strongener containing bees

The invention relates to a strong and strong sperm, a sexual function improving agent, a male menopausal symptom improving agent and an anti-fatigue agent.

One problem that men face about aging is the discovery of male menopause. It is currently estimated that about 40% of men from 40 to 60 generations have experienced some degree of male menopause.

Entering male menopause, you will feel a variety of symptoms (eg depression, depression, irritability, restlessness, nervousness, fatigue, loss of life, sarcoma, arthritis, decreased strength, fever, sweating, memory loss, sleep disorders, concentration Reduced power, physical exhaustion, erectile dysfunction (ED), decreased libido, loss of ejaculation, etc.). As a method for confirming whether or not it is a male menopausal disorder, there is a method of measuring blood testosterone concentration by blood test and a questionnaire. At present, it is pointed out that the incidence of menopausal disorders in men is extremely related to the various stresses in men and women in work and family. In recent years, there has been a tendency for males in high years to increase into menopausal disorders.

Male climacteric disorder is based on the age-reducing androgen reduction, and there are autonomic dysfunction symptoms and neuropsychiatric symptoms similar to female climacteric disorders, and in most cases it will show further complication of male dysfunction (Okuyama Akiko) Book male menopause LOH syndrome I male menopause D. From the point of view of cardiotherapy, 24 pages (Nanshan Hall, Inc.) 2007.). On the other hand, the secretion of sex hormones, which is the first of the sex hormones, is regulated by the hypothalamic-pituitary-seed nest with negative feedback to regulate the secretion of hormones containing steroids (Osama Akira's book menopausal disorder LOH syndrome II male menopause diagnosis C. Hormone determination and evaluation, 65 pages (Nanshan Hall, 2007). Therefore, it is difficult to judge male menopausal disorders based only on the measured values of steroids.

In general, subjects with reduced self-consciousness and a tendency to reduce free steroids in the blood will be diagnosed as age-related male hypogonadism syndrome (LOH syndrome) and adapted to androgen replacement therapy in order to maintain QOL. However, hormone replacement therapy has been popularized internationally with respect to women, and treatment for men is not only medically popular except for the widespread use of EDs using PDE5 inhibitors.

For example, Patent Documents 1 to 3 have reported various components for raising steroids.

Patent Document 1 reports that the Tangshinoside, Lancemaside-A, and Syringin contained in the Codonopsis genus have an inhibitory effect on blood steroids.

Patent Document 2 reports that geranylgeranol has a steroid enhancing effect.

Patent Document 3 reports black vinegar-related materials and combining the materials with specific materials can significantly promote the secretion of steroids.

However, androgen replacement therapy also has the potential to cause side effects, particularly prostate-specific antigens (PSAs) for prostate tumor markers that require special attention, and therefore there is a need for a menopausal symptom-lowering agent that has no androgenic effects.

In addition, "sildenafil" which is a PDE5 inhibitor is used as an ED therapeutic drug system. However, although sildenafil is transient, side effects and drug interactions have been reported. example For example, for the blood pressure lowering action of a nitric acid agent such as nitroglycerin, since sildenafil promotes the action, it is contraindicated and used together, and in addition, its effect on the eye nitrile may be considered to have blue vision, double vision, Red vision and so on.

Here, the bee has been eaten as a nutrient source such as protein in various countries and regions since ancient times. In recent years, it has been known to use a powder obtained by pulverizing and drying a bee as a raw material. This healthy food is rich in protein, amino acids, vitamins and minerals found in bees. Further, as disclosed in Patent Document 4, the bee is known to have an antioxidant effect, but most of the other effects are not known.

[Previous Technical Literature] [Patent Literature]

[Patent Document 1] Japanese Patent Laid-Open Publication No. 2006-306889

[Patent Document 2] Japanese Patent No. 5382212

[Patent Document 3] Japanese Patent Laid-Open Publication No. 2013-181031

[Patent Document 4] Japanese Patent No. 5405067

An object of the present invention is to provide a strong and strong sperm, a sexual function improving agent, a male menopausal symptom improving agent, and an anti-fatigue agent which do not cause an androgen to rise and which are highly safe.

The present inventors repeated the results of the intensive research in order to achieve the above object, and obtained the off The bee has strong and strong effects, sexual function improvement, men's menopausal symptoms and anti-fatigue effects, and the results of high safety are obtained by the fact that the bees can not increase androgen.

The present invention is based on such knowledge and is further researched and repeated, and provides the following strong and strong sperm, sexual function improving agent, male menopausal symptom improving agent and anti-fatigue agent.

Item 1. A strong and strong agent containing bees.

Item 2. A sexual function improving agent containing bees.

Item 3. A male menopausal symptom improving agent containing bees.

Item 4. An anti-fatigue agent containing bees.

Item 5. An enhancer containing bees.

Item 6. The strong and strong agent according to Item 1, the sexual function improving agent according to Item 2, the male menopausal symptom improving agent according to Item 3, the anti-fatigue agent according to Item 4, and the item 5 as described in Item 5 The activator, wherein the bee is treated with a protein degrading enzyme.

Item 7. A composition containing strong and strong use of bees, improvement of sexual function, improvement of male menopausal symptoms, use of anti-fatigue and/or vitality.

Item 8. A food composition containing strong and strong use of bees, improvement of sexual function, improvement of male menopausal symptoms, use of anti-fatigue and/or vitality.

Item 9. The composition according to Item 7 or 8, wherein the bee is an egg White matter decomposing enzyme processor.

Item 10. A method for strengthening and strengthening, a method for improving sexual function, a method for improving male menopausal symptoms, a method for improving fatigue, and a method for improving vitality, comprising the step of administering an effective amount of a bee to a mammal.

Item 11. The method according to Item 10, wherein the bee is treated with a proteolytic enzyme.

Item 12. The use of a strong and strong sperm, a sexual function improving agent, a male menopausal symptom improving agent, an anti-fatigue agent, and a booster in the manufacture of a booster.

Item 13. The use according to Item 12, wherein the bee is treated with a proteolytic enzyme.

Because the bee has the effect of strengthening and strengthening the body, improving the sexual function, improving the menopausal symptoms and anti-fatigue effect, it can be suitable as a strong and strong agent, a sexual function improving agent, a male menopausal symptom improving agent and anti-fatigue. The active ingredient of the agent. Further, the bee can increase the androgen and has high safety.

Fig. 1 is a graph showing (a) AMS score (total), (b) AMS score (sex factor), and (c) AMS score (physical factor) before and after ingestion of test food. Mean ± SE * p < 0.05 ** p < 0.01

Fig. 2 is a graph showing IIEF5 before and after ingestion of test foods. Mean ± SE * p < 0.05 ** p < 0.01

Fig. 3 is a graph showing the number of erections in the morning before and after ingestion of the test food. Mean ± SE ^* p < 0.05 ^** p < 0.01

Fig. 4 is a graph showing the fatigue feeling VAS before and after ingestion of the test food. Mean ± SE ^* p < 0.05 ^** p < 0.01

Fig. 5 is a graph showing SDS before and after ingestion of test foods. Mean ± SE ^* p < 0.05 ^** p < 0.01

Fig. 6 is a graph showing (a) TT and (b) FT before and after ingestion of test foods. Mean ± SE p < 0.05

Hereinafter, the present invention will be described in detail.

The strong and strong sperm, the sexual function improving agent, the male menopausal symptom improving agent and the anti-fatigue agent of the present invention are characterized by containing bees.

The strong and strong essence agent, the sexual function improving agent, the male menopausal symptom improving agent and the anti-fatigue agent of the present invention have high safety without increasing the androgen. .

Androgens, also known as hormonal hormones for male hormones, include, in addition to the representative steroids, dihydroguanosterone, dehydroepiandrosterone, androstenone, androstenedione, epiandrosterone, and the like.

The bee here refers to the larvae and mites of the bees. There are no special restrictions on the types of bees. They can be widely used: Japanese honey bees, western honey bees, bees, African bee-like bees, wasps (including blacks). Vespa, bees, bumblebees, etc., known bees. It is preferable to use bees, and it is preferable to use western honey bees from the viewpoint of easy availability. Further, although it may be male or female, it is preferably a drone.

The larvae and mites are not particularly limited as long as they are hatched by the eggs. Among them, it is preferred to use larvae and pupa of bees that have been hatched for 16 to 23 days after hatching, and it is preferable to use larvae and pupa of bees that have been hatched for 18 to 21 days after hatching.

Bees can accumulate nutrients in the body. In particular, drones have been used as materials for the Han Dynasty since ancient times, and their various amino acids containing essential amino acids not only exist in a balanced manner, but are also rich in proteins, lipids, sugars, vitamin B group, folic acid, and nicotine. A vitamin group such as acid or pantothenic acid, and a mineral such as zinc or selenium. The physiological activities and pharmacological effects of the bee are known to have antibacterial, anti-inflammatory, antiviral, antiprotozoal, and tinnitus-eliminating effects.

The bee of the present invention uses raw bees and raw bees in a processed state. The processing of the bee, specifically, comprises: pulverized bees (raw or dried); dried bees (raw or pulverized); heated treated bees (raw, dried or pulverized) Extraction of bees using water, aqueous ethanol, or the like. Preferably, it is a dry powder of the bee prepared by pulverizing the bee (raw).

The term "bee" according to the present invention includes, unless otherwise specified, a raw bee, a processed product which is dried, pulverized or heat-treated, and a bee is extracted using water, aqueous ethanol or the like (raw, The obtained product of the dried product and the pulverized material, and the obtained product obtained by treating the bee and its processed product with a proteolytic enzyme (peptidase).

The drying method may be a conventional method used in general food processing, such as natural drying such as air drying, sun drying, forced drying by electric heating or the like, freeze drying, or the like. It is preferably freeze-dried. Further, the drying time is not particularly limited, and for example, In the case of natural drying such as ventilation or drying in the sun for about 3 days, the forced drying by heating with electricity or the like is carried out at about 50 ° C for about 1 to 3 days. Usually, the water content is 10% by mass or less, and preferably the drying amount is 5% by mass or less. In the case of natural drying such as ventilation or drying, when the water content is difficult to be 10% by mass or less, the freeze dryer can be further used to carry out the treatment for lowering the water content.

The pulverization treatment (pulverization treatment) can be carried out by a conventional method such as a method using a pulverizer (rolling mill) or a method of crushing it using a mortar.

The method of heating the bees is not particularly limited, and a heat treatment method using 70 to 120 ° C is preferably used. The bees (including raw, dried and pulverized materials) can be easily put into boiling water for heat treatment. In order to avoid the dissolution of various active ingredients such as vitamins and amino acids as much as possible, and heat treatment, a method of steaming the bees may be preferably used.

For the extraction method, the following method can be used: a method of adding water, aqueous ethanol, etc. to a bee (including raw, dried, and ground matter), stirring, and obtaining a supernatant by centrifugation; and filtering the filtrate to obtain a filtrate. .

By treating the proteolytic enzyme, the protein contained in the bee and its processed product can be reduced in molecular weight, thereby obtaining an allergic reaction caused by the protein, and the enzyme treatment can be suppressed (hypoallergenic enzyme treatment) ()).

The proteolytic enzyme used for the enzyme treatment of the bee and its processed material can be suitably enumerated as a peptidase. The peptidase to be used may have at least one of an endopeptidase action and an exopeptidase action.

Examples of proteolytic enzymes having endopeptidase activity include animals derived from animals (eg, trypsin, chymotrypsin, etc.), plants (eg, papain, etc.), and sources. Endopeptidase from microorganisms (eg, lactic acid bacteria, yeast, mold, Bacillus subtilis, actinomycetes, etc.).

Examples of the proteolytic enzyme having exopeptidase activity include a carboxypeptidase, an aminopeptidase, or a peptidase derived from a microorganism (for example, lactic acid bacteria, pullulans, Rhizopus, etc.) or Trypsin, pepsin, etc. with endopeptidase activity.

Preferred examples of the enzyme having both exopeptidase activity and endopeptidase activity in such various enzymes include peptidase produced by Streptomyces griseus (trade name: actinase AS) and rice. Peptidase (trade name: protease A, flavor protease) produced by Aspergillus oryzae, and peptidase (trade name: protease P) produced by Aspergillus melleus.

Further, preferred examples of the enzyme having an exopeptidase action include peptidase produced by Aspergillus oryzae (trade name: umami enzyme G, Promod 192P, Promod 194P, Sumizyme FLAP), and Aspergillus soygium (Aspergillus). The peptidase produced by sojae (trade name: Sternzym B15024), the peptidase produced by the genus Fusarium (trade name: Kokulase P), and the peptidase produced by Rhizopus oryzae (trade name: peptidase R).

Further, preferred examples of the peptidase having an endopeptidase action include peptidase produced by Bacillus subtilis (trade name: orientase 22BF, Nukureishin), and Bacillus licheniformis (pacillus licheniformis). Trade name: alkaline protease), peptidase produced by Bacillus stearothermophilus (trade name: protease S), peptidase produced by Bacillus amyloliquefaciens (trade name: neutral protease), Peptidase produced by Bacillus (trade name: Protamex) and the like.

In order to reduce the allergic enzyme treatment of the bee and its processed product, it can be carried out, for example, according to the description of JP-A-2009-159997.

The strong and strong essence agent, sexual function improving agent and male menopausal symptom improvement of the invention The agent and the anti-fatigue agent may contain additional components as long as they do not inhibit the effects of the present invention. When the relevant additional ingredients are included, the ratio of the strong and strong sperm, the sexual function improving agent, the male menopausal symptom improving agent, and the anti-fatigue agent can be exemplified as 0.01 to 99% by mass, preferably 1 to 80% by mass. More preferably, it is 10 to 70% by mass, and further preferably 20 to 50% by mass.

The form of the strong and strong essence agent, the sexual function improving agent, the male menopausal symptom improving agent and the anti-fatigue agent of the present invention is not particularly limited, and may be, for example, a powder, a granule, a tablet, a capsule, a liquid, or a suspension. A formulation form such as a liquid or emulsion.

The strong and strong agent, the sexual function improving agent, the male menopausal symptom improving agent, and the anti-fatigue agent of the present invention can be suitably used as a component of a food composition, a pharmaceutical product, and a pharmaceutical product (particularly, an oral drug).

The strong and strong essence agent, the sexual function improving agent, the male menopausal symptom improving agent and the anti-fatigue agent of the present invention include foods and drinks for the purpose of health care, health maintenance, promotion, etc. (for example, health food, functional food, nutrition) Composition (nutritional composition), nutritional supplement foods, supplements, health foods, specific health foods, nutritious functional foods, or functional foods), pharmaceutical products, and pharmaceuticals (specially, oral drugs). In addition, the strong and strong essence agent, sexual function improving agent, male menopausal symptom improving agent and anti-fatigue agent of the invention also include: imparting strong and strong sperm function, improving sexual function, improving male menopausal symptoms, and anti-fatigue effect. And additives to enhance vitality.

The above food and beverage, if necessary, can be combined with excipients, brighteners, minerals, vitamins, flavonoids, terpenoids, polyphenols, amino acids, nucleic acids, essential fatty acids, cooling agents, binding agents, sweet Flavoring agents, disintegrating agents, lubricants, coloring agents, perfumes, stabilizers, preservatives, sustained release modifiers, surfactants, solubilizers, wetting agents, and the like.

The type of the above-mentioned foods and drinks is not particularly limited, and examples thereof include beverages (coffee, juice, refreshing drinks for tea drinks, milk drinks, lactic acid bacteria drinks, yogurt drinks, carbonated drinks, Japanese sake, wine, fruit wine). Etc.); sauces (casta cream, etc.); sauces (fruit jams, etc.); cakes (chocolate, donuts, pies, puffs, chewing gum, jellies, candies, biscuits, cakes, puddings, etc.) Japanese dim sum (Dafu, cake, taro, Casita, stuffed honey, lamb, etc.); frozen desserts (ice cream, popsicle, Xuebao, etc.); food (curry, burdock, chowder, miso soup) , soup, meat sauce, Italian noodles, pickles, jams, etc.); seasonings (sauce, mixed with rice, flavorants, soups, etc.).

The dosage unit form when used as a supplement is not particularly limited, and may be appropriately selected, and examples thereof include a tablet, a capsule, a granule, a liquid, a powder, and the like.

The proportion of the bee contained in the above-mentioned foods and drinks may be, for example, 0.01 to 99% by mass, preferably 1 to 80% by mass, more preferably 10 to 70% by mass, still more preferably 20 to 50% by mass. .

The intake of the above-mentioned foods and drinks can be appropriately set depending on various conditions such as the weight, age, sex, and symptoms of the ingested person.

The strong and strong agent, the sexual function improving agent, the male menopausal symptom improving agent, and the anti-fatigue agent of the present invention can be referred to as a bee preparation when prepared as a pharmaceutical product or a pharmaceutical product. For the external medicines or pharmaceuticals of the Ministry of Medicine, the above-mentioned bees may be used alone, or other pharmaceutical ingredients described in the Japanese Pharmacopoeia such as vitamins and crude drugs may be mixed.

The strong and strong agent, the sexual function improving agent, the male menopausal symptom improving agent and the anti-fatigue agent of the present invention can be used as a non-toxic carrier and diluted by the bee and the pharmaceutical product when being prepared as a pharmaceutical product or a pharmaceutical product. Or an excipient, together with a tablet (including a tablet, a sugar coating) Ingots, foamed ingots, film-coated tablets, chewable tablets, oral tablets, capsules, pills, powders (granules), fine granules, granules, liquids, suspensions, emulsions, syrups, syrups The type of the drug becomes an orally administrable preparation.

The dosage of the above-mentioned medical product or the pharmaceutical product can be appropriately set according to various conditions such as the weight, age, sex, and symptoms of the patient. Ideally, the bee of one day can be 0.5 g or more, preferably 0.7 g. More preferably, it is 1 g or more.

The ratio of the bee contained in the pharmaceutical product or the pharmaceutical product is not particularly limited as long as it can exert the effects of the present invention, and is, for example, 0.01 to 99% by mass, preferably 1 to 80% by mass, more preferably It is 10 to 70% by mass, and more preferably 20 to 50% by mass.

The strong and strong sizing agent, sexual function improving agent, male climacteric symptom improving agent and anti-fatigue agent of the present invention described above are suitable for use in mammals including humans (preferably humans, particularly males).

The food and beverage, the external medicine and the pharmaceutical product of the invention have the functions of strengthening and strengthening the sperm, improving the sexual function, improving the menopausal symptoms of the male, anti-fatigue effect and enhancing the vitality. Further, since the foods and drinks, the medical products, and the pharmaceuticals of the present invention do not increase the androgen, they have high safety.

[Examples]

Hereinafter, the present invention will be described in further detail by way of examples. However, the invention is not limited to the embodiments.

[Test example]

<subject>

Questionnaires and examinations (health examinations, doctor consultations, blood tests, questionnaires, etc.) are (1) men over the age of 35 and under 60 years old, and (2) male menopause consultation forms (below AMS) 27 points or more 49 The following points, (3) free steroids were 8.5pg/mL or more and less than 11.8pg/mL, (4) married or heterosexual subjects, a total of 11 subjects.

<Test method>

The subject was 11 people, and the soft capsule-shaped bees contained in the 12-week continuous daily contain 6 food capsules, one of which contained 180 mg of bee. The nutrients of one capsule of the test food capsule are shown in the table below.

Further, the subject recorded the symptoms including the presence or absence of morning erection, life information, and the like from 1 week before the start of the test to the end of the ingestion.

At the beginning of the trial and at the 12th week after the start of the trial, doctors' examinations, questionnaires, health examinations, blood tests, and urine tests were performed to evaluate total steroids (hereinafter referred to as TT), free steroids (hereinafter referred to as FT), and aging men's symptoms. Ticket (hereinafter referred to as AMS), Self-rating depression scale (SDS), International Erectile Function Scale Scale (hereinafter referred to as IIEF5) and fatigue sensation VAS. In addition, the number of morning erections during the trial period was also assessed. project.

The AMS score is a self-reported questionnaire widely used in the symptomatic consultation of LOH syndrome. The composition is divided into psychological factors 5 items (25 points), physical factors 7 items (35 points), and sexual function factors 5 items (25). Point) Total 17 items (85 points). All items are "none", "slight", "normal" and "serious" The "very serious" 5-stage assessment is scored individually by 1 to 5 points. The higher the score, the more severe the symptoms.

The SDS system is an indicator of the degree of depression, and the depressive symptoms are formed by 20 questions. The higher the score, the more severe the symptoms.

The IIEF5 series is divided into five items by the International Index of Erectile Function (IIEF), which is an international indicator used for screening and treatment of ED effects. The lower the score, the more severe the symptoms.

According to the VAS method, the left end of the straight line of 10cm is "the best feeling that you will never feel tired from the experience without experience"; the right end is "the worst feeling from the fatigue you have never experienced to the total inability to act". The position X is recorded at the position on the straight line, which is the value of the distance from the left end to the recorded position. When the value is lowered, the fatigue is improved.

<Statistical Analysis>

The mean and standard deviation of each group of data were obtained, and the difference in the number of Wilcoxon digits was determined by AMS score, IIEF5, SDS, morning erection times and fatigue sensation VAS.

TT, FT, health check, blood test and blood biochemical test, the corresponding t test.

The statistical analysis of either method uses a two-tailed test with a hazard rate of 5% and 1%.

<Result>

1 Effectiveness assessment project

1) AMS

The AMS score, when compared to the start of ingestion, can be invented at the end of the intake (Figure 1-a) "Sexual Factors" (Figure 1-b) "Physical Factors" (Figure 1-c).

2) IIEF5

A comparison of the pre- and post-intake of the test foods revealed a significant increase in IIEF5 at the end of ingestion (Figure 2).

3) Morning erection times

In the comparison before and after the test food intake, it was found that the number of morning erections increased meaningfully at the end of the intake (Fig. 3).

4) Fatigue VAS

In the comparison before and after the test food intake, it was found that the sense of fatigue was meaningfully decreased at the end of the intake (Fig. 4).

5) SDS

In the comparison before and after the test food intake, it was found that the depressive symptoms were meaningfully decreased at the end of the intake (Fig. 5).

2 Safety assessment project

Confirmation of TT and FT (Figure 6), health check, blood test, blood biochemical test and urine test for each subject did not reveal any clinical changes.

During the test period, the subjects did not find any symptoms. In addition, during the ingestion period, no abnormalities were found.

< Conclusion>

11 people with less than 35 years of age and 60 years of vitality in daily life, and 6 capsules of soft capsules containing bee food on the 1st, a meaningful decline in AMS, meaningful increase in IIEF5 and morning erections, fatigue assessment The VAS and SDS questionnaires found a meaningful decline. On the other hand, there was no change in total free ketamine values. That is, the bee does not increase the value of the testosterone, and it is found to have an improvement effect on male menopausal symptoms, an anti-fatigue effect, and a compression-resistance effect.

Prescription example

Hereinafter, a typical example of the present invention will be described.

Prescription Example 1 Soft capsule

1/6 of the amount of bees of 1080 mg, plus edible fats, beeswax, and soybean plant-derived lecithin, and filled into soft capsules containing gelatin and caramel color to obtain a soft capsule preparation.

Prescription Example 2 Soft capsule

180mg of bee, mixed with edible oils, γ-aminobutyric acid, glycerin, soybean plant-derived lecithin, beeswax, vitamin B2, and vitamin B12, and filled into soft capsules containing gelatin and caramel color to obtain soft Capsule preparation.

Prescription Example 3 Soft capsule

100mg of bee, mixed with edible fat, maca extract powder, Dongge Ali extract powder, Cordyceps sinensis mycelium powder, zinc-containing yeast, sputum extract powder, beeswax, and soybean plant-derived lecithin To a soft capsule containing gelatin and cocoa pigment, a soft capsule preparation is obtained.

Claims (4)

A strong and strong agent containing bees. A sexual function improving agent containing bees. A male menopausal symptom improving agent containing bees. An anti-fatigue agent containing bees.