JP2003277273A - Mineral absorption-promoting agent - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a composition which has an excellent mineral absorption- promoting action, can widely be used in various fields, is free from side effects, and doesn't have a problem on safety. <P>SOLUTION: An agent, medicine, food, beverage or feedstuff having a mineral absorption-promoting action contains yeast cell walls or yeast cell wall polysaccharides as an active ingredient. <P>COPYRIGHT: (C)2004,JPO

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to a novel use of yeast cell wall or yeast cell wall polysaccharides. More specifically, the present invention uses yeast cell wall or yeast cell wall polysaccharides as an active ingredient and has an excellent effect of promoting mineral absorption. And food and pharmaceutical compositions having Further, the present invention relates to beverages and animal feeds having a mineral absorption promoting action.

[0002]

2. Description of the Related Art Conventionally, calcium intake of Japanese people is lower than nutritional requirement. Insufficient intake of calcium causes osteoporosis and osteomalacia. In particular, when an elderly person has a femoral neck fracture or a lumbar compression fracture, the probability of becoming bedridden is high, and it is said to be a major social problem in the coming aging society. In addition to calcium, minerals such as zinc and magnesium tend to be deficient. Deficiency of zinc causes poor growth, skin disorders, taste disorders and the like. Magnesium is necessary to maintain the enzymatic activity in the body and is involved in all physiological functions. Magnesium deficiency is considered to be one of the causes of cardiovascular diseases, osteoporosis, osteomalacia and the like. To compensate for these mineral deficiencies, it is necessary to ingest more minerals than required per day, but it is more efficient to increase the absorption rate of minerals than to increase the intake amount. Therefore, foods with high absorption efficiency of minerals that tend to be deficient,
Also, there is great interest in substances that promote absorption.

As such a substance, Japanese Patent Laid-Open No. 6-205
654 discloses a mineral absorption enhancer containing a branched galacto-oligosaccharide as an active ingredient, which can enhance the absorption of minerals by oral administration and is effective in preventing or treating osteoporosis and other bone diseases. As food
Japanese Patent Application Laid-Open No. 7-241172 discloses a casein phosphopeptide and zinc obtained by allowing trypsin to act on casein,
A mineral absorption-promoting food or drink containing one of trace elements of iron and copper is disclosed. In addition, JP-A-9-6725
No. 7 discloses a composition for promoting absorption of minerals containing chitin as an active ingredient. Japanese Unexamined Patent Publication No. 2000-270812 discloses a mineral absorption promoter containing κ-casein glycomacropeptide as a mineral absorption promoter in foods and drinks. However, there was no mineral absorption promoter focused on the yeast cell wall.

[0004]

DISCLOSURE OF THE INVENTION The present inventors have paid particular attention to iron among minerals, and have previously found that yeast cell wall or yeast cell wall polysaccharide has a mineral absorption promoting action and an anemia preventing or ameliorating action. (Japanese Patent Application 2000-0
52882). The present invention further studies calcium, magnesium, zinc, etc., and a mineral enhancer containing yeast cell wall or yeast cell wall polysaccharide as an active ingredient has no side effect and is highly safe, and has an action of promoting absorption of these minerals. I found that.

[0005]

Therefore, the present invention is an absorption promoter for minerals excluding iron, which comprises yeast cell wall or yeast cell wall polysaccharides as an active ingredient. Preferred minerals are calcium, magnesium and zinc. The present invention also relates to foods, beverages, feeds and pharmaceutical compositions having an action of promoting absorption of minerals other than iron, which contains yeast cell wall or yeast cell wall polysaccharides as an active ingredient.

[0006]

BEST MODE FOR CARRYING OUT THE INVENTION The yeast cell wall used in the present invention is prepared by subjecting live yeast to autolysis or enzyme treatment with the addition of protease, nuclease, esterase, glucanase, lipase, etc. It is obtained from the extraction residue obtained by removing. As the yeast used at this time, those generally used in the food industry such as brewer's yeast, baker's yeast, alcohol yeast, and sake yeast are used. It is desirable to use brewer's yeast, baker's yeast, and torrula yeast because of easy availability of raw materials. In order to rapidly perform the enzyme treatment, the cell wall may be subjected to physical disruption with a homogenizer or the like before obtaining the cell residue. If necessary, yeast cell wall can be obtained by washing the yeast, adjusting pH, adjusting temperature or pressure, and the like.

Alternatively, the yeast cell wall obtained by removing the soluble intracellular components of yeast may be treated with an acid or alkaline aqueous solution to prepare a yeast cell residue from which the soluble components have been removed. Specifically, the yeast cell wall is 0.
Treat with acid such as 01 to 2N hydrochloric acid, sulfuric acid, nitric acid, or 0.01
After treatment with an alkali such as ˜2N sodium hydroxide or potassium hydroxide, the suspension can be prepared by collecting the yeast cell residue by centrifugation or the like. Heating may be performed during the acid or alkali treatment. Alternatively, the yeast cell residue can be obtained by washing with a solvent such as ethanol after treating with acid or alkali.

The yeast cell wall polysaccharide used in the present invention is DJM.
anners et al. (Biochem. J., 135, 19 (1973)), DHNorthc
oat et al. (Biochem. J. 51, 232 (1952)), Maeda et al.
4-66546). The method for collecting yeast cell wall polysaccharides is not limited to this method. Yeast cell wall and yeast cell wall polysaccharides are commonly used carriers,
It can be formulated with auxiliary agents, additives and the like, and can be used as an oral product according to a conventional method as a pharmaceutical product for animals including humans. Moreover, it can be mixed as a food material for animals including humans and used as food and drink thereof.

When used as a medicine, the mineral absorption promoter containing yeast cell wall as an active ingredient can be used as an oral preparation such as a preparation, capsule, granule, syrup and the like. For example, in the case of tablets, 2 mg per day
An amount of ˜1000 mg / kg body weight, preferably 10 mg to 200 mg / kg body weight is administered once or several times, and the effect can be sufficiently exerted.

The medicinal product of the present invention can take the required unit volume form together with physiologically acceptable vehicles, carriers, excipients, integrators, stabilizers, flavoring agents and the like. The adjuvants mixed in tablets and capsules are as follows. Binders such as trogant, gum arabic, corn starch, gelatine, swelling agents such as microcrystalline cellulose, lubricants such as magnesium stearate, sweeteners such as sucrose, lactose, saccharin, peppermint, red oil,
Flavoring agents such as cherry. In the case of capsules, the above materials may further contain a liquid carrier such as oil and fat, and other materials may be used as a coating agent or the physical form of the preparation may be changed by another method. . For instance, tablets may be coated with shellac, sugar. Syrup or erythicyl agent as sweetener, sucrose,
Preservatives may contain methyl or propylparaben, dyes and flavoring agents such as cherry or orange flavor.

The food and drink of the present invention may be in the form of the above-mentioned preparation, but may be processed and manufactured by a general manufacturing method by adding a necessary amount to each food material in the form of candy, rice cracker, cookie, beverage and the like. . Ingestion as a health food or functional food is used for disease prevention and health maintenance, so it is divided into several times a day as an oral intake and the total daily dose is 0.1g-100g,
It is preferably ingested as a processed product containing 0.5 to 20 g.

[0012]

INDUSTRIAL APPLICABILITY Since the present invention uses a substance derived from a natural product containing yeast cell wall or yeast cell wall polysaccharide as an active ingredient, it has no side effects, is highly safe and is excellent in processability.
It is possible to provide foods, beverages, feeds, and pharmaceutical compositions having a mineral absorption promoting action for facilitating absorption of various minerals in the living body.

[0013]

EXAMPLES Next, the present invention will be described in detail with reference to examples, but the present invention is not limited to these examples. Experimental Example 1: Production of yeast cell wall The yeast cell wall was brought into contact with brewer's yeast slurry for 50 seconds with an activated soda solution adjusted to pH 10, the debittered one was washed with water, and left overnight at pH 3 to 8 and 50 to 70 ° C. Completely self-digested, water-solubilized components were extracted and removed with water, and spray-dried. From 10 kg of 10% yeast slurry, 460 g of yeast cell wall was obtained.

Experimental Example 2: Production of yeast cell wall polysaccharides 1 kg of the yeast cell wall obtained in Experimental Example 1 was treated with solvent water at atmospheric pressure for 10
After heat treatment at 0 ° C. for 16 hours, the water-soluble fraction obtained by centrifugation (8000 rpm, 15 minutes) was filtered through Celite. The filtrate was concentrated under reduced pressure (concentration of about 20 to 30 times) and then left at 4 ° C. for one day to remove the cold solidified product. To the concentrated solution, twice the amount of ethanol was added to obtain a polysaccharide component precipitate. The precipitate was freeze-dried to obtain 13 g of white powder.

Example 1: Examination of absorption promoting action of minerals The yeast cell wall obtained in Experimental Example 1 was used. The examination was carried out using 4-week-old male Wistar rats. After preliminary breeding for 1 week,
The test feeds shown in Table 1 were freely given from the age of 5 weeks. The mineral content in each feed was adjusted in consideration of the amount of mineral contained in the yeast cell wall (Table 2). Feces from 10 days to 14 days after the start of feed administration were collected for 4 days, and the amount of each mineral excreted in the feces was measured. The apparent absorption rate was calculated by the following formula. Apparent absorption rate (%) = [(mineral amount in feed ingested-mineral amount in excreted feces) / (mineral amount in ingested feed)] × 100 Apparent absorption rate of each mineral is shown in Table 3. It was shown to. The test group promoted absorption of each mineral as compared with the control group.

[0016]

[Table 1]

[0017]

[Table 2]

[0018]

[Table 3] Table 3 Apparent absorption rate (%) of Ca, Mg and Zn ―――――――――――――――――――――――― Test group Absorption rate (%) Ca Mg Zn ―――――――――――――――――――――――― Control group 73.2 ± 4.45 79.3 ± 2.38 24.4 ± 3.67 Test group 78.3 ± 5.49 ^* 84.2 ± 3.65 ^** 26.4 ± 16.8 ―――――――――――――――――――――――― (n = 8, mean ± standard deviation, ^* p <0.05, ^** p <0.01)

Example 2: Solubilization of minerals It was examined using 4-week-old Wistar male rats. 1
After the preliminary breeding for a week, the test feeds shown in Table 4 were freely given from the age of 5 weeks. The mineral content in each feed was adjusted in consideration of the amount of mineral contained in the yeast cell wall (Table 5). Feed administration
After dissection on the 14th day to remove the cecum, the contents of the cecum were taken out, and the content pH was measured. In addition, the contents are centrifuged (10 mi
n, 10,000 × g) to separate the supernatant and the precipitate, measure the amount of minerals in each fraction, and determine the ratio of the amount of solubilized minerals in the supernatant to the amount of minerals in the cecal contents (possible) The solubilization rate) was determined. The pH of the cecal contents is shown in Table 6. The test group had a decreased pH of the contents as compared with the control group. Also,
The solubilization rate of each mineral is shown in Table 7. The test group had a higher solubilization rate for all minerals than the control group.

[0020]

[Table 4]

[0021]

[Table 5]

[0022]

[Table 6] Table 6 Cecal contents pH ――――――――――――― Test group pH ――――――――――――― Control group 6.79 ± 0.28 Test group 5.37 ± 0.11 ――――――――――――― (N = 4, mean ± standard deviation)

[0023]

[Table 7] Table 7 Solubilization rate of minerals in the cecum (%) ――――――――――――――――――――――――――― Test group Solubilization rate (%) ) Ca Mg Zn ――――――――――――――――――――――――――― Control group 6.74 ± 0.97 28.5 ± 5.59 3.90 ± 1.92 Test group 21.2 ± 6.01 50.3 ± 7.93 19.7 ± 12.2 ――――――――――――――――――――――――――― (n = 4, mean ± standard deviation)

Example 3 Application of Yeast Cell Wall to Pharmaceutical Products 1) Tablet and Capsule Formulation Yeast Cell Wall 10.0 g Lactose 75.0 g Magnesium Stearate 15.0 g Total 100.0 g The above parts by weight were uniformly mixed and tableted according to a conventional method. , As a capsule.

2) Powders and granules Yeast cell wall 20.0 g Starch 30.0 g Lactose 50.0 g Total 100.0 g The above parts by weight were uniformly mixed and made into powder and granules according to a conventional method.

Example 4: Application of yeast cell wall to foods 1) Candy yeast cell wall 0.10 g sucrose 19.30 g starch syrup 70.00 g water 9.00 g colorant 0.55 g flavor 0.05 g total 100.0 g The above parts by weight are uniformly mixed. , Candy according to the usual method.

2) Cookie yeast cell wall 1.0 g soft flour 30.0 g corn starch 10.0 g whole egg 15.0 g butter 16.0 g sugar 22.0 g water 5.8 g baking powder 0.2 g total 100.0 g The above-mentioned parts by weight are uniformly mixed, and then according to a conventional method. I used cookies.

Example 5: Application of yeast cell wall polysaccharides to foods 1) Soup mannan 1.0 g Sweet corn (cream type) 19.0 g Butter 4.0 g Wheat flour 3.0 g Broth soup 32.5 g Milk 32.5 g Fresh cream 8.0 g Total 100.0 g The above parts by weight were uniformly mixed and made into a soup according to a conventional method. The mannan used was produced from yeast cell wall polysaccharides.

2) Juice Mannan 1.00 g Concentrated mandarin orange juice 15.00 g Fructose 5.00 g Citric acid 0.20 g Flavor 0.10 g Pigment 0.15 g Sodium ascorbate 0.05 g Water 78.50 g Total 100.0 g Juice was made according to the law. The mannan used was produced from yeast cell wall polysaccharides.

─────────────────────────────────────────────────── ─── Continuation of front page (51) Int.Cl. ⁷ Identification code FI theme code (reference) A61K 47/36 A61K 47/36 47/46 47/46 A61P 3/12 A61P 3/12 3/14 3 / 14 F term (reference) 2B150 DC14 DD11 4B018 LB01 LB08 LB10 LE01 LE05 MD81 ME14 MF03 MF04 MF05 MF06 MF11 4C076 AA30 AA36 AA53 BB01 CC21 EE30N EE56N FF34 4C086 AA01 AA02 HA21 HA04 NA11 ZC

Claims (7)

[Claims] 1. An absorption enhancer for minerals excluding iron, which comprises yeast cell wall or yeast cell wall polysaccharides as an active ingredient. 2. The absorption enhancer according to claim 1, wherein the mineral is calcium. 3. The absorption enhancer according to claim 1, wherein the mineral is magnesium. 4. The absorption enhancer according to claim 1, wherein the mineral is zinc. 5. A pharmaceutical composition containing the absorption enhancer according to any one of claims 1 to 4. 6. A beverage containing the absorption enhancer according to any one of claims 1 to 4. 7. A feed containing the absorption enhancer according to any one of claims 1 to 4.