TW201412340A - Oligosaccharide peptides with whitening activity, manufacturing method thereof and whitening formulation thereof - Google Patents

Abstract

The present invention discloses oligosaccharide peptides with whitening activity, a manufacturing method thereof and a whitening formulation thereof. The manufacturing method of the oligosaccharide peptides with whitening activity comprises the following steps: extracting and precipitating mushroom-like plants to obtain polysaccharide peptides; and adding hydrolase in the polysaccharide peptides for conducting hydrolysis of the polysaccharide peptides to obtain oligosaccharide peptides. The molecular mass of the oligosaccharide peptides is less than 3000 Dalton. Wherein, the tyrosinase activity inhibition analysis is conducted with the oligosaccharide peptides, and the tyrosinase activity inhibition rate of the oligosaccharide peptides reaches 97% when additions of the oligosaccharide peptides is 0.3mg/mL.

Description

Oligosaccharide peptide having whitening function, preparation method thereof and whitening formula thereof

The invention relates to an oligosaccharide peptide with whitening function, a preparation method thereof and a whitening formula thereof, in particular to a method for hydrolyzing a polysaccharide extracted from a natural mushroom mites to form a molecular mass with an enzyme to enhance whitening. Functional oligosaccharide peptides, methods for their preparation, and whitening formulations thereof.

For modern women, they are very beautiful, ancient people, beauty is a woman's nature, and it is quite a requirement for the skin, and women's skin will produce pigmentation, relaxation or depression due to age and congenital or acquired environment. The dark yellow complexion, and the ability to have a bright, soft, firm skin is the ultimate state of every woman's dream.

At present, the ingredients commonly used in the market or used by popular women for whitening and whitening can be divided into two categories, one is chemical and medical whitening ingredients, including hydroquinone, steroids, arbutin, tranexamic acid, Rhododendron acid, ascorbic acid, A acid, placenta, hormones, etc., although it has a whitening effect, may be accompanied by unexpected side effects. The other is the use of natural plant extracts of blemish whitening ingredients, such as licorice extract, mulberry extract, coix seed extract, saxifrage extract, angelica extract, etc., also inhibit tyrosinase in melanocytes. Efficacy can slow down the production of melanin.

In addition, recent studies have found that polysaccharide peptides extracted from natural mushroom mites have physiological activities similar to those of polysaccharides, such as regulating immune function, anti-inflammatory, anti-tumor activity, anti-oxidation, anti-virus, anti-aging, scavenging free radicals. , detoxification, lowering cholesterol and other effects. Because polysaccharide peptides have many beneficial effects, they are often regarded as beneficial additives, added to foods or health care products, etc., and can be applied to cosmetic raw material formulations to increase the functionality and value of cosmetic products, such as whitening functions. .

However, in general, the extraction methods of polysaccharide peptides are mostly carried out by liquid fermentation culture of the mycelium of mushroom fungi or directly by using fruit bodies, and extraction by hot water extraction or acid-base extraction, and further by organic solvents, such as The alcohol or the like is subjected to precipitation purification to obtain a polysaccharide peptide. The molecular weight of the polysaccharide peptide extracted by this conventional method is relatively large, and the human body can not easily absorb it, and if it is applied to a cosmetic formula, it may not be able to achieve a better whitening effect of the cosmetic. Therefore, the inventors of the present invention have made improvements in light of the disadvantages of the prior art.

In view of the above problems of the prior art, the object of the present invention is to provide an oligosaccharide peptide having a whitening function, a method for producing the same, and a whitening formula thereof, so as to solve the molecular mass of the polysaccharide peptide extracted by the natural mushroom mites. It is not easy to be absorbed by the human body, so it can not be used in cosmetic raw materials to achieve better whitening function.

According to the object of the present invention, an oligosaccharide peptide having a whitening function is proposed, which extracts a polysaccharide peptide by extracting and precipitating a mushroom, and adding a hydrolysis peptide to a hydrolysis enzyme to obtain a hydrolysis process. An oligosaccharide peptide. The molecular mass of the oligosaccharide peptide is less than 3000 Daltons (Dalton, Da). Among them, the oligosaccharide peptide was subjected to a tyrosinase activity inhibition assay, and when the amount of the oligosaccharide peptide added was 0.3 mg/mL, the tyrosinase activity inhibition rate of the oligosaccharide peptide was 97%.

Preferably, the weight percentage of the polysaccharide peptide and the hydrolyzed enzyme may be 99.8 to 99 wt% and 0.2 to 1 wt%, respectively.

Preferably, the mushroom plant may comprise Yunzhi, Antrodia camphorata, Flammulina, Ganoderma lucidum, Brassica or shiitake mushroom.

Preferably, the hydrolyzed enzyme may comprise β-1,3-D-glucanase (β-1,3-D-glucanase).

In accordance with the purpose of the present invention, there is further proposed a whitening formula comprising an oligosaccharide peptide having a whitening function as described above.

According to the object of the present invention, a method for producing an oligosaccharide peptide having a whitening function is further provided, which comprises the steps of: extracting a mushroom mites to obtain an initial extract; and precipitating the preliminary extract by an electrolytic method; a polysaccharide peptide; and adding a hydrolyzing enzyme to the polysaccharide peptide to form a mixture, and subjecting the mixture to a hydrolysis process at a predetermined temperature to obtain an oligosaccharide peptide.

Preferably, the molecular weight of the oligosaccharide peptide is less than 3000 Daltons (Dalton, Da).

Preferably, the weight percentage of the polysaccharide peptide and the hydrolyzed enzyme may be 99.8 to 99 wt% and 0.2 to 1 wt%, respectively.

Preferably, the mushroom plant may comprise Yunzhi, Antrodia camphorata, Flammulina, Ganoderma lucidum, Brassica or shiitake mushroom.

Preferably, the hydrolyzed enzyme may comprise β-1,3-D-glucanase (β-1,3-D-glucanase).

Preferably, the predetermined temperature may comprise 20 to 40 °C.

Preferably, the method for producing an oligosaccharide peptide having a whitening function according to the present invention further comprises the following steps: after the mixture is subjected to a hydrolysis procedure, a freeze-drying operation is further performed to obtain an oligosaccharide peptide.

Preferably, the method for producing an oligosaccharide peptide having whitening function according to the present invention further comprises the following steps: performing an activity inhibition inhibition assay of oligosaccharide peptide, wherein the amount of oligosaccharide peptide added is 0.3. At mg/mL, the tyrosinase activity inhibition rate of the oligosaccharide peptide was 97%.

According to the present invention, the oligosaccharide peptide having the whitening function, the method for producing the same, and the whitening formula thereof can be obtained by hydrolyzing the polysaccharide peptide extracted from the natural mushroom genus plant to the oligosaccharide having a small mass of the component. Peptide. The oligosaccharide peptide can be easily absorbed by the human body, and can be applied to a cosmetic formula to inhibit tyrosinase activity in the skin melanocytes, thereby reducing the production of melanin to achieve the whitening function of the product.

The features, aspects, and advantages of the invention, as well as the advantages thereof, will be apparent to those skilled in the art.

Please refer to FIG. 1 , which is a flow chart of a method for producing a whitening-functional oligosaccharide peptide of the present invention. In the figure, the method for producing an oligosaccharide peptide having a whitening function comprises the following steps: First, step S11: extracting a mushroom mites to obtain an initial extract. Next, step S12: using a precipitation method to precipitate a preliminary extraction of a polysaccharide peptide. Finally, step S13: adding a hydrolyzed enzyme to the polysaccharide peptide to form a mixture, and subjecting the mixture to a hydrolysis process at a predetermined temperature to obtain an oligosaccharide peptide.

In the above, the mushroom genus may include Yunzhi, Antrodia camphorata, Flammulina velutipes, Ganoderma lucidum, Brazilian mushrooms or shiitake mushrooms. The precipitation method may include an organic solvent precipitation method such as an alcohol (ethanol) precipitation method or the like. The hydrolyzing enzyme may comprise β-1,3-D-glucanase (β-1,3-D-glucanase). The weight percentage of the polysaccharide peptide and the hydrolyzed enzyme may be 99.8 to 99 wt% and 0.2 to 1 wt%, respectively. The above are only preferred embodiments, but should not be limited thereto.

In the above, the present invention specifically performs an extraction and hydrolysis process for Yunzhi to obtain a Yunzhi oligosaccharide peptide having a small molecular weight, and is applied to a cosmetic formulation to enhance the whitening function of the product. Among them, Yunzhi's scientific name is Trametes versico or Coriolus versicolor, which is a basidiomycete, a layer of fungi, a non-lepidaceous, a polyporaceae, a genus of the genus Coriolus, which is parasitic on rot, which is a white mycelium. It is widely distributed in the world and is a widely used medicinal fungus. The Yunzhi polysaccharide peptide produced by liquid culture contains 18 kinds of amino acids, 70% of which are acidic and neutral amino acids, such as aspartame. Acid, succinic acid, serine, glutamic acid, glycine, alanine, valine and leucine.

In the above, the polysaccharide chain obtained from Yunzhi has a molecular mass of about 100 kDalton (Dalton, Da). In order to make the polysaccharide peptide more easily absorbed by the human body and increase its applicability, the present invention will be 1 mg/ The mL polysaccharide peptide was subjected to a biohydrolysis reaction by adding 15 mL of 1 mL of hydrolyzed enzyme to hydrolyze the polysaccharide peptide into an oligosaccharide peptide having a molecular mass of less than 3000 Daltons (Dalton, Da). Further, in step S13, a freeze-drying operation is performed after the hydrolysis process to prepare an oligosaccharide peptide. The most suitable environment in the hydrolysis procedure may be pH 7.0, and the predetermined temperature may comprise 20 to 40 ° C, and the optimum predetermined temperature may be 30 ° C. The above are merely exemplary embodiments and should not be construed as limiting.

Please refer to Figures 2 to 3, which are graphs showing the inhibition rate of in vitro tyrosinase activity of the whitening-functional oligosaccharide peptide of the present invention and the inhibition of tyrosinase IC ₅₀ value in vitro. In particular, the present invention is directed to extracting Yunzhi to form a Yunzhi polysaccharide peptide, and further subjecting the Yunzhi polysaccharide peptide to a hydrolysis process to obtain a Yunzhi oligosaccharide peptide having a small molecular weight. In order to confirm that the Yunzhi oligosaccharide peptide of the present invention has whitening ability, it can inhibit the activity of tyrosinase in the skin melanocytes to reduce melanin production, and thus the Yunzhi polysaccharide peptide and the Yunzhi oligosaccharide peptide are commercially available with whitening. functions at arbutin and kojic acid for the ability to inhibit tyrosinase outer one test and one outer tyrosinase inhibition IC ₅₀ value of the test.

In the above, the experimental results of inhibiting the tyrosinase ability test in vitro showed that the tyrosinase activity inhibition rate (%) of Yunzhi oligosaccharide peptide was superior to that of the arbutin which has whitening ability in the market. And when the concentration of Yunzhi oligosaccharide peptide is 0.2mg/mL, it can be clearly observed that the inhibition rate of tyrosinase activity is similar to that of whitening function, and a small amount of Yunzhi oligosaccharide peptide can be added to achieve rapid inhibition. The ability of tyrosinase. Similarly, the tyrosinase activity inhibition rate (%) of the Yunzhi oligosaccharide peptide produced after the hydrolysis is better than that of the Yunzhi polysaccharide peptide before the hydrolysis, as shown in FIG. 2 . Among them, the results showed that when the addition amount of oligosaccharide peptide was 0.3 mg/mL, the inhibition rate of tyrosinase activity of oligosaccharide peptide was 97%, and the inhibition rate of tyrosinase activity was better than that of commercially available whitening ingredient. . The results of this experiment prove that the oligosaccharide peptide has the ability to inhibit tyrosinase activity, reduce melanin production, and achieve whitening effect.

Among the above, in the results of inhibiting the tyrosinase IC ₅₀ value in vitro, when Polysaccharide peptides (PSP), Arbutin, Kojic acid, and Oligosaccharide peptides, When the inhibition rate of tyrosinase activity of OSP) reaches 50% (IC ₅₀ ), the concentration of each component to be added can be observed. Among them, Yunzhi oligosaccharide peptide added concentration only needs 189.03mg/mL to achieve 50% tyrosinase activity inhibition ability, better than the commercially available arbutin tyrosinase activity inhibition rate, such as the third The figure shows. This proves that the Yunzhi oligosaccharide peptide has the ability to inhibit tyrosinase activity, thereby reducing melanin production and achieving whitening effect.

It can be known from experiments that the oligosaccharide peptide has the advantages of being small in molecular weight and easily absorbed by the human body, and having the same tyrosinase activity inhibition as arbutin and citric acid. In the cosmetic formula, the whitening function of the cosmetic product can be enhanced to provide the user with effective inhibition of the activity of tyrosinase in the melanocytes of the skin, thereby reducing the production of melanin in the body and achieving the whitening effect.

In summary, the oligosaccharide peptide having the whitening function of the present invention, the method for producing the same, and the whitening formula thereof, are obtained by extracting a natural mushroom mites to form a primary extract, and then using the primary extract The precipitation method is to precipitate a polysaccharide peptide, and then the polysaccharide peptide is added to a hydrolyzing enzyme to carry out a hydrolysis process to hydrolyze it into an oligosaccharide peptide having a small molecular mass. In this way, the oligosaccharide peptide can be easily absorbed by the human body, and if it is applied to cosmetics as a raw material for whitening, thereby enhancing the whitening effect and applicability of the product.

The above is intended to be illustrative only and not limiting. Any equivalent modifications or alterations to the spirit and scope of the invention are intended to be included in the scope of the appended claims.

S11~S13. . . step

Fig. 1 is a flow chart showing a method for producing a whitening-functional oligosaccharide peptide of the present invention.
Fig. 2 is a graph showing the analysis of the inhibition rate of in vitro tyrosinase activity of the oligosaccharide peptide having whitening function of the present invention.
Fig. 3 is a graph showing the analysis of the in vitro inhibition of tyrosinase IC ₅₀ value of the whitening-functional oligosaccharide peptide of the present invention.

S11~S13. . . step

Claims (13)

An oligosaccharide peptide having a whitening function, which comprises extracting a polysaccharide peptide by extracting and precipitating a mushroom, and adding the hydrolysis peptide to a hydrolysis enzyme to carry out a hydrolysis process to obtain an oligosaccharide peptide. The molecular mass of the oligosaccharide peptide is less than 3000 Daltons (Dalton, Da);
Wherein, the oligosaccharide peptide is subjected to a tyrosinase activity inhibition assay, and when the oligosaccharide peptide is added in an amount of 0.3 mg/mL, the tyrosinase activity inhibition rate of the oligosaccharide peptide is 97%. . The oligosaccharide peptide having whitening function as described in claim 1, wherein the weight percentage of the polysaccharide peptide and the hydrolyzed enzyme is 99.8 to 99 wt% and 0.2 to 1 wt%, respectively. The oligosaccharide peptide having whitening function as described in claim 1, wherein the mushroom genus comprises Yunzhi, Antrodia, Flammulina, Ganoderma lucidum, Brassica or shiitake mushroom. The oligosaccharide peptide having a whitening function as described in claim 1, wherein the hydrolyzing enzyme comprises β-1,3-D-glucanase (β-1,3-D-glucanase). A whitening formula comprising an oligosaccharide peptide having a whitening function as described in any one of claims 1 to 4. A method for producing an oligosaccharide peptide having a whitening function, comprising the following steps:
Extracting a mushroom mites to obtain an initial extract;
And extracting a polysaccharide peptide by an initial precipitation method; and adding a hydrolyzing enzyme to the polysaccharide peptide to form a mixture, and subjecting the mixture to a hydrolysis process at a predetermined temperature to obtain an oligosaccharide peptide . The method for producing a whitening-functional oligosaccharide peptide according to claim 6, wherein the oligosaccharide peptide has a molecular mass of less than 3000 Daltons (Dalton, Da). The method for producing an oligosaccharide peptide having a whitening function according to claim 6, wherein the weight percentage of the polysaccharide peptide and the hydrolyzed enzyme is 99.8 to 99 wt% and 0.2 to 1 wt%, respectively. The method for producing an oligosaccharide peptide having a whitening function according to claim 6, wherein the mushroom plant comprises Yunzhi, Antrodia, Flammulina, Ganoderma lucidum, Brassica or shiitake mushroom. The method for producing an oligosaccharide peptide having a whitening function according to claim 6, wherein the hydrolyzed enzyme comprises β-1,3-D-glucanase (β-1,3-D-glucanase). The method for producing a whitening-functional oligosaccharide peptide according to claim 6, wherein the predetermined temperature comprises 20 to 40 °C. The method for producing an oligosaccharide peptide having whitening function as described in claim 6 of the patent application further comprises the following steps:
After the mixture is subjected to a hydrolysis procedure, a freeze-drying operation is further performed to obtain the oligosaccharide peptide. The method for producing an oligosaccharide peptide having whitening function as described in claim 6 of the patent application further comprises the following steps:
The oligosaccharide peptide was subjected to a tyrosinase activity inhibition assay. When the amount of the oligosaccharide peptide added was 0.3 mg/mL, the tyrosinase activity inhibition rate of the oligosaccharide peptide was 97%.