TW201340991A - Composition for improving skin defensive power - Google Patents

Abstract

Disclosed is a composition for improving skin defensive power containing at least one of a protopanaxadiol compound and a Japanese nutmeg extract as an active ingredient.

Description

a composition that enhances skin protection

The present invention relates to a composition for enhancing skin protection.

Tea tree oil, salicylic acid, triclosan, fruit acid and the like which have antibacterial activity itself have long been used to prevent or ameliorate acne or skin problems. However, since the antibacterial activity of these substances may cause skin irritation and there are regulatory restrictions on the permitted concentration, the concentrations used by these substances are not effective and difficult to achieve substantial results. Therefore, there is a need to develop a substance that exhibits excellent antibacterial and anti-inflammatory effects without irritating the skin.

[Related technology]

[Patent Literature]

(Patent Document 1) Korean Patent No. 10-0884616 (February 12, 2009);
(Patent Document 2) Korean Patent Application Publication No. 10-2006-0043067 (May 15, 2006).

【technical problem】
The present invention aims to provide a composition capable of enhancing skin protection.

【Technical solutions】
In a large aspect, a composition for enhancing skin protection is provided, which comprises at least one of a protopanaxadiol compound and a Torreya nucifera extract as an active ingredient.

In another general aspect, a composition for enhancing skin protection is provided, the composition comprising at least one of a protopanaxadiol compound and a Japanese eucalyptus extract (Torreya nucifera); Epigallocatechin gallate is used as an active ingredient.

[Beneficial effect]
The composition of the present invention contains at least one of a protopanaxadiol compound and an extract of Japanese eucalyptus, and optionally an epigallocatechin gallate, which can be promoted by the compound The antibacterial peptide is active and promotes skin self-protection to exhibit excellent skin antibacterial and anti-inflammatory activity.

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Figure 1 compares the effects of Compound K, Japanese eucalyptus seed oil, epigallocatechin gallate, a mixture of the above, and the performance of tea tree oil on the antibacterial peptide in the skin.

[best embodiment]
As used herein, the term "skin" means the tissue that covers the surface of an animal and, in its broadest sense, includes not only the face or body, but also the scalp and hair. As used herein, the term "extract" broadly includes any material extracted from a natural product, regardless of the extraction method employed, the extraction solvent, the extracted component, or the type of extract. For example, the extract can be an oil component extracted from a natural product. As used herein, the term "skin defensive power" means the ability to protect the skin from external stimuli or toxins. The stimulus broadly includes any irritating effects on the skin, including bacteria, viruses, ultraviolet light (UV), dryness, damage, and the like.

The invention is described in detail below.

The skin resists bacterial attack by secreting antibacterial peptides. Therefore, a substance which can promote the activity of the antibacterial peptide in the skin can enhance the antibacterial and anti-inflammatory ability of the skin without irritating the skin by enhancing the protective effect of the skin.

In another aspect, the present invention provides a composition for enhancing skin protection comprising at least one of a protopanaxadiol compound and a Japanese eucalyptus extract as an active ingredient. The protopanaxadiol compound or the Japanese eucalyptus extract can enhance skin protection by promoting the performance of the antibacterial peptide in the skin. Therefore, the composition containing at least one of the protopanaxadiol compound and the Japanese eucalyptus extract can improve skin protection, particularly, enhance the antibacterial and anti-inflammatory activity of the skin, and can further improve the acne and other skin problems. In an exemplary embodiment of the invention, the antibacterial peptide is a peptide that induces expression in response to external stimuli and is capable of controlling invading pathogens. Representative examples include hBD1, hBD2, LL37 or RNA hydrolase 7 (RNase7), but are not limited thereto.

In an exemplary embodiment of the invention, the protopanaxadiol compound is a ginsenoside which is a ginseng saponin. In another exemplary embodiment of the present invention, the ginseng diol compound may be obtained by treating the ginseng extract, especially the red ginseng extract, with an enzyme. In another exemplary embodiment of the invention, the protopanaxadiol compound comprises Compound K. Compound K is also known as ginsenoside M1, which is a 20-O-(bd-glucopyranoside)-20(S)-protopanaxadiol represented by Chemical Formula 1 (20-O-(beta-d-glucopyranosyl) )-20(S)-protopanaxadiol).

[Chemical Formula 1]

The composition according to an exemplary embodiment of the present invention may comprise 0.001 to 10% by weight, preferably 0.01 to 5% by weight, more preferably 0.1 to 3% by weight, based on the total weight of the composition, of the protopanaxadiol compound. When the protopanaxadiol compound of the above range is contained, the desired effects of the present invention can be suitably attained while satisfying the stability and safety of the composition. In addition, the above range can also be obtained with appropriate cost-effectiveness. In particular, if the composition has a protopanaxadiol compound content of less than 0.001% by weight, the effect of enhancing skin protection may be negligible. Further, if the content of the original panaxadiol compound of the composition exceeds 10% by weight, the composition may lower the solubility of the composition due to a decrease in the solubility of the original panaxadiol compound in the solvent.

In an exemplary embodiment of the invention, the Japanese eucalyptus extract comprises a dendritic extract of Japanese eucalyptus (Torreya nucifera), especially an extract of Japanese eucalyptus seed. In another exemplary embodiment of the present invention, the Japanese eucalyptus extract includes oils and fat components, such as Japanese eucalyptus oil obtained by pressing or using an organic solvent to extract Japanese eucalyptus, especially Japanese eucalyptus seeds. oil. The organic solvent includes one or more organic solvents selected from the group consisting of ethanol, hexane, acetone, ethyl acetate, diethyl ether, ethyl methyl ketone, and chloroform, but is not limited thereto. this.

In an exemplary embodiment of the present invention, the composition may contain 0.001 to 99% by weight, preferably 0.1 to 80% by weight, more preferably 10 to 50% by weight, of Japanese eucalyptus, based on the total weight of the composition. Extracts. When the Japanese eucalyptus extract in the above range is contained, the desired effects of the present invention can be suitably attained while satisfying the stability and safety of the composition. In addition, the above range can also be obtained with appropriate cost-effectiveness. In particular, if the Japanese eucalyptus extract content of the composition is less than 0.001% by weight, the effect of enhancing skin protection may be extremely small.

The composition for enhancing skin protection according to an exemplary embodiment of the present invention may further comprise epigallocatechin gallate. When the skin-protecting composition of the present invention further contains epigallocatechin gallate, the composition may have a better effect due to a higher skin antibacterial and anti-inflammatory activity enhancing effect. Skin protection enhances the effect.
In the present invention, epigallocatechin gallate is a polyphenolic compound and is mainly present in catechins in green tea, and is also (-)-epigallocatechin-3- The chemical formula of gallic acid ester ((-)-epigallocatechin-3-gallate) is shown. In an exemplary embodiment of the invention, epigallocatechin gallate can be extracted from green tea.

The compound according to an exemplary embodiment of the present invention may contain 0.00001 to 10% by weight, preferably 0.0001 to 5% by weight, more preferably 0.001 to 1% by weight, based on the total weight of the compound, of epigallocatechin. Acid ester. When the epigallocatechin gallate is contained in the above range, the desired effects of the present invention can be suitably attained while satisfying the stability and safety of the composition. In addition, the above range can also be obtained with appropriate cost-effectiveness. In particular, if the epigallocatechin gallate content of the composition is less than 0.00001% by weight, the effect of enhancing skin protection may be extremely small. Further, if the epigallocatechin gallate content of the composition exceeds 10% by weight, the oxidation reaction tends to occur, and it is difficult to ensure the stability of the formulation.

In another aspect, the present invention provides a cosmetic composition comprising at least one of a protopanaxadiol compound and an extract of Japanese eucalyptus; and an epigallocatechin gallate, optionally selected The ester acts as an active ingredient. The cosmetic composition exhibits a skin-protecting effect, particularly exhibiting excellent antibacterial and anti-inflammatory activity, and the cosmetic composition can further improve or prevent acne or other skin problems.

The cosmetic composition of the present invention provided may be any formulation suitable for external use. For example, the cosmetic composition can be prepared in the form of a solution, an oil-in-water emulsion, a water-in-oil emulsion, a suspension, a solid, a gel, a powder, a paste, a foam or an aerosol. Such formulations can be prepared according to methods commonly used in the art.

The cosmetic composition of the present invention may further comprise a humectant, an emollient, a surfactant, an ultraviolet (UV) absorber, a preservative, a bactericide, an antioxidant, a pH control agent, an organic or inorganic A pigment, a fragrance, a cooling agent or an antiperspirant. Those skilled in the art can readily determine the amount of such ingredients in the cosmetic composition, and the range of such ingredients will not adversely affect the objects and effects of the present invention. The content of the components may be from 0.01 to 5% by weight, preferably from 0.01 to 3% by weight, based on the total weight of the composition.

In another aspect, the present invention provides a pharmaceutical composition comprising at least one of a protopanaxadiol compound and an extract of Japanese eucalyptus, and optionally epigallocatechin gallate. The ester acts as an active ingredient. The pharmaceutical composition exhibits a skin-protecting effect, particularly exhibiting excellent antibacterial and anti-inflammatory activity, and the pharmaceutical composition can further improve or prevent blistering and other skin problems.

The pharmaceutical composition according to one aspect of the present invention can be administered orally or parenterally (for example, rectal, topical, transdermal, intravenous, intramuscular, intraperitoneal or subcutaneous).

Formulations for oral administration include, but are not limited to, tablets, tablets, hard or soft capsules, granules, powders, granules, liquids, emulsions or pellets. These formulations may contain, in addition to the active ingredient, diluents (for example, lactose, dextrose, sucrose, mannitol, sorbitol, cellulose or glycine), lubricants (for example, silica, Talc, stearic acid or polyethylene glycol) or binder (for example, aluminum magnesium citrate, starch paste, gelatin, tragacanth, methylcellulose, sodium carboxymethylcellulose or polyvinylpyrrolidone ). The pharmaceutical composition may also contain pharmaceutical additives such as disintegrating agents, absorbents, coloring agents, perfumes, sweeteners, and the like, as occasion demands. Tablets can be prepared by conventional mixing, granulating or film coating methods.

Formulations for parenteral administration include, but are not limited to, injections, pharmaceutical drops, lotions, ointments, gels, creams, suspensions, emulsions, stoppers, patches or sprays. .

The active ingredient of the pharmaceutical composition according to one aspect of the present invention may be administered in a dose corresponding to the age, sex, weight of the patient, the particular disease or pathological condition to be treated, the severity of the disease or pathological condition, and the route of administration. And the doctor's considerations change. Taking these factors into account to determine the dosage is a level of competence of those skilled in the art. The daily dose may be, for example, 0.1 to 100 mg/kg/day, preferably 5 to 50 mg/kg/day, but is not limited thereto.

[Inventions]
The invention will be described in detail below by way of examples and test examples. However, the following examples are for illustrative purposes only, and it is apparent to those skilled in the art that the scope of the invention is not limited by the embodiments.

[Examples]
Compound K is obtained by hydrolyzing red ginseng with an enzyme. Japanese eucalyptus seed is extracted by pressing Japanese eucalyptus seeds or using an organic solvent to obtain Japanese eucalyptus seed oil.

Epigallocatechin gallate (EGCG) was obtained according to the following method. First, the green tea leaves were extracted by hot water reflux at 10 to 70 ° C for 1 to 24 hours using 10 volumes of pure water. After filtration, the residue was removed, and the remaining filtrate was filtered again to separate the water-soluble component. From the separated water-soluble components, only epigallocatechin gallate is selectively precipitated and concentrated by crystallization. After the unprecipitated residual filtrate was removed by filtration, the precipitate was purified by chromatographic analysis. Subsequently, the solvent was evaporated, and finally high-purity epigallocatechin gallate was obtained.

[Test Example] Evaluation of the effect of improving the performance of antibacterial peptides in the skin

Use compound K, Japanese eucalyptus seed oil, epigallocatechin gallate (EGCG), compound K + Japanese eucalyptus seed oil, compound K + Japanese eucalyptus seed oil + epigallocatechin Gallate or tea tree oil was treated with HaCaT keratinocytes (provided by Dr. NE Fusenig of the German Cancer Research Center in Heidelberg, Germany) for 24 hours, using real-time PCR. The changes in gene expression of representative skin antibacterial peptides hBD1, hBD2, LL37 and RNA hydrolase 7 (RNase7) were measured. Fig. 1 (average value) and Table 1 (mean ± standard deviation value) show changes in gene expression of each of the treated groups and the untreated control group.

[Table 1]

It can be seen that compound K, Japanese eucalyptus seed oil, epigallocatechin gallate (EGCG), compound K + Japanese eucalyptus seed oil, compound K + Japanese eucalyptus seed oil + table gallop The tea gallate can enhance the performance of the skin antibacterial peptides hBD1, hBD2, LL37 and RNase7, while the tea tree oil from natural products and commonly used in antibacterial cosmetics inhibits the expression of these four genes. That is, although tea tree oil does not enhance skin protection, compound K, Japanese eucalyptus seed oil, epigallocatechin gallate (EGCG), and mixtures thereof can be achieved by activating antibacterial in the skin. Peptides enhance skin protection.

An example of a formulation of a composition according to an exemplary embodiment of the present invention will be described in detail below, however, the example can be applied to various different types of formulations. The following examples of formulations are not intended to limit the scope of the invention, but are merely illustrative.
[Formulation Example 1] Emulsion

The emulsion was prepared according to the composition contained in Table 2 according to a usual method.

[Table 2]

[Formulation Example 2] Cream

Creams were prepared according to the compositions contained in Table 3 according to the usual methods.

[table 3]

[Formulation Example 3] Mask

The mask was prepared according to the composition contained in Table 4 according to a usual method.

[Table 4]

[Formulation Example 4] Soft Capsules

The original ginseng diol compound (50 mg), Japanese eucalyptus extract (200 mg), epigallocatechin gallate (10 mg), soy extract (50 mg), large Soybean oil (180 mg), palm oil (2 mg), hydrogenated palm oil (8 mg), yellow beeswax (4 mg) and lecithin (6 mg) were mixed and prepared into soft capsules.

[Formulation Example 5] Lozenges

Mixed compound K (10 mg), Japanese eucalyptus seed oil (200 mg), epigallocatechin gallate (10 mg), soy extract (50 mg), glucose (100 mg), starch (96 mg) and magnesium stearate (4 mg), and 30% ethanol (40 mg) was added to the mixture to form granules. After the granules were dried, the granules were prepared into tablets using a tablet machine.

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Claims (10)

A composition for enhancing skin protection, comprising at least one of a proto-ginseng diol compound and a Japanese eucalyptus extract (Torreya nucifera) as an active ingredient. The composition of claim 1, wherein the protopanaxadiol compound comprises the compound K (20-O-(b-d-glucopyranoside)-20(S)-protopanaxadiol). The composition of claim 1, wherein the Japanese eucalyptus extract comprises Japanese eucalyptus seed oil. The composition of claim 1, wherein the composition comprises 0.001 to 10% by weight of the protopanaxadiol compound based on the total weight of the composition. The composition of claim 1, wherein the composition comprises 0.001 to 99% by weight of the Japanese eucalyptus extract based on the total weight of the composition. The composition of claim 1, wherein the composition further comprises epigallocatechin gallate. The composition of claim 6, wherein the composition comprises 0.00001 to 10% by weight of epigallocatechin gallate, based on the total weight of the composition. The composition of claim 1, wherein the composition enhances antibacterial and anti-inflammatory activity of the skin. The composition of claim 1, wherein the composition promotes the performance of an antimicrobial peptide in the skin. The composition of any one of claims 1 to 9, wherein the composition is a cosmetic composition.