201204417 六、發明說明： 【發明所屬之技術領域】 本發明係關於一種寄生於養殖魚之鰓吸蟲、體表吸蟲等外 部寄生蟲之驅除方法。 【先前技術】 近年來，海產魚類養殖技術發達，青甘鰺、獅魚、比目魚、 真鯛、河豚等之高級魚成為養瘦對象。養瘦場中，為了縮短 養殖期間、提升商品價值，重要的是預防病毒、病原性細菌、 外部寄生蟲所造成之疾病。然而，在成為適合外部寄生蟲繁 殖之水溫的5月〜8月（水溫22°C〜27°C)，時常發生養殖魚的 大量死亡。即使未到達死亡的程度，仍因寄生蟲而使養殖魚 體力減少、攝食不良，喪失商品價值。一般受到指摘是在禁 用有機錫系之防藻劑後，並未對於寄生蟲被害之增加開發出 有效之驅除方法乃原因所在。而在養魚用調配飼料中混合藥 劑、進行經口投予的市售藥劑雖然已普及，但仍只對一部分 寄生蟲確認到效果。作為寄生蟲代表，有如體表面寄生之體 表吸蟲與魚鰓葉寄生之鰓吸蟲之二種。 作為養殖場之寄生蟲的驅除方法，迄今檢討出以下方法。 (1)淡水浴法、濃鹽水浴法 所謂淡水浴法、濃鹽水浴法，係指藉由在事先準備於船槽 等中之淡水或濃鹽水中使魚進行浸潰處理數分鐘，使寄生蟲 因滲透壓改變而衰弱，並從魚體脫落、死亡。此方法雖然因 100113338 4 201204417 處理水之鹽濃度與海水不同而對魚體亦造成影響，且必須將 用水搬運至實施的養殖場，但因無需擔心海水污染，故可說 得上是適當方法。 (2)藥浴法 藥浴法係欲利用過氧化焦磷酸鈉、過碳酸鈉、過氧化填酸 二鈉、甲醛、冰醋酸等藥劑之作用，將寄生蟲從魚體驅除。 然而’藥劑有污染周邊海水的問題。作為藥浴法，習知係進 行下述方法。 專利文獻1中，揭示有藉由阿魏酸與乳酸，由感染了體表 吸蟲等寄生蟲之魚驅除寄生蟲。 專利文獻2中，揭示有藉由投予以可可豆組成物作為有效 成分的寄生蟲抑制劑，以抑制及預防海產養殖魚之寄生蟲症 的方法。 專利文獻3中’揭示有將δ-胺基戊酮酸添加於飼料或水 槽中，以治療感染了病原性微生物及寄生蟲之魚類的方法。 專利文獻4中’作為寄生於虎河豚之總吸轰之驅除劑及驅 除方法，揭示有使用苯并咪唑系藥劑作為有效成分。 專利文獻5中’揭示有使用穩定化二氧化氯或亞氯酸鹽、 有機羧酸及過氧化氫，以治療及預防魚類之盾狀纖毛蟲感染 症(Scuticociliatosis)的方法。盾狀纖毛嘉因較深地侵入東體 内，故與其他寄生蟲病不同’無法期待以藥浴進行驅蟲。習 知，一直以來慣用的曱醛已全面禁止使用於養殖魚。 100113338 5 201204417 (3)過氧化氫法 另外亦有使用下 作為寄生蟲之驅蟲方法或感染預防方法， 述過氧化氫的方法。 專利文獻6中，作為提供完全不伴隨習知方法般之不利、 困難’即使在大量處理時仍操作容易且可效率佳地進行處 理，以驅除寄纽養❹、之寄生蟲的方法，係射有將海水 系養殖場之養殖缝容於魚_之錢婦泳區域中，投予 稀釋為適當濃度的過氧化氫水溶㈣方法。專利文獻6中， 作為較佳範圍係推薦於過氧化氫水溶液濃度 400〜1000PPm、處理時間3〜10分鐘之範圍内選擇實施。= 而，搬送過氧化氫水溶液之濃厚藥液相當危險。而且，若經 常使用過氧化氫，將可增強寄生蟲對過氧化氫的财受性。 專利文獻7中，作為海水養殖場中虎河豚之異溝盤蟲 (Heterobothrium)(蘇吸蟲）症的預防方法，揭示有於過氧化氣 濃度400ppm以上不對魚體造成藥害的濃度以下、處理時 間20分鐘以上的條件下’對魚體進行處理，而可將寄生於 鰓之階段的異溝盤蟲驅除。 專利文獻8中，揭示有添加過氧化氫至過氧化氫濃度成為 10〜600ppm，於維持上述濃度之狀態下’依上述閉鎖水系統 之水面的照射強度為2〜3〇mW/cm2之方式照射240〜370nm 之紫外線3〜40分鐘，以殺滅養殖魚之寄生蟲卵的方法。 另外，專利文獻9中，係以過醋酸作為有效成分，添加過 100113338 6 201204417 氧化虱至成為8〇ppm至2000ppm之濃度後，再以驗中和， 於此處理液中對養殖魚進行浸潰處理，藉此於較寬之溫度區 域下不對養殖魚造成傷害，而嘗試養殖魚的寄生蟲驅除。 (4)其他方法 作為其他方法，專利文獻10、U中，揭示有—種光觸媒 反應水生錢置’錢由於含_子.水驗中使對光觸 媒體照射料線所產生之雜氧種擴散，㈣水_活性氧 種之機能，利錢齡之氧化反錢進行微生物之 生蟲之驅蟲、原蟲類之驅除。錢，為 ⑭β 蟲死滅，必須藉由1外線使寄生 專利^ 養料中的利用。 專利文獻12中，作為為了抑制養殖备 的繁死發生，使魚類之免賴活化心^寄生蟲症所造成 生蟲症之免⑽活的枝，揭村’、尤其是使魚類寄 階段，缺乏實用性。 “、、用疫苗，但尚屬研究 (5)臭氧水所進行之方法 非專利文獻1巾，記载有屬於氧化 微生物的殺菌效果。 臭氧對於魚類病原 非專利文獻2中，揭示有臭氧水 理，於濃度〇.3〜0.5ppmT5〜1〇分^合飼育用水、用具處 育槽中，為了避免與海水中漠化物間里：有效果。然而，於飼 養殖魚的影響，必須將濃度減低至百八反應所造成之漠酸對 非專利文獻3中，報告有藉㈣。 100113338 ’依〇.3ppm、5分鐘 201204417 之處理可防止角孢子蟲（Ceratomyxa)症、黏液孢子蟲 (Myxozoa)屬寄生蟲感染症的實例。 尚且’在海水中存在溴化物離子的情況，由於其與臭氧反 應而生成具有殘留性、有害性的溴酸離子，故最好事先予以 去除。 如上述’習知之上述臭氧水所進行的方法中，有關於作為 本發明對象之養殖魚中所寄生之外部寄生蟲的驅除方法，尤 其是寄生於如虎珂豚般外皮較厚之魚類的鰓吸蟲、體表吸蟲 等之外部寄生蟲的驅除，仍屬未知。 洛解了臭氧氣體之臭氧水，&由美國FDA(食品醫藥品局） 登錄於食品添加物表中’已獲得作為食品貯藏、製造步驟中 之殺菌劑的認可(2GG1年）。且於食品4内之殺g、食品本 身之殺菌方面已有多數實際績效。 臭氧水之優點有如： (a) 臭氧(經由OH自由基)殺菌效果為細胞壁之氧化破壞且無 差別性，故不存在耐性菌。 (b) 由於會分解為氧，故無有害之二次生成物。 (c)無殘留性。 無殘留性既為優點，亦為缺點。若可將臭氧氣體穩定保 於溶液中，則可期待其應用、效果的擴大。 另外，對於人體之臭氧療法有悠久歷史，而以有益之治 方法又到矚目最近’如非專利文獻4所記載般，已進展 100113338 201204417 對家畜、寵物的利用。最近的岍究曰 氧將使免疫系統滅活。對於養殖备月，’主入至血液中的臭 能性。 亦有表現此種效果的可 臭氧水習知以來一般係使用故， 行製造，可輕易製造數ppm 型之臭氧氣體產生器進 〈臭氧水， 艮品洗淨領域中。然而，放畲 利用於淨水處理、 苞裂之臭惠^ 由而有使用領域的限制。 、氣體產生器因以下理 (a) 若使用乾燥空氣作為原料，則a 到高濃度氣體，必須使用純氧原料而產生出N〇x。為了得 (b) 必須有使臭氧先以氣體型式產生 的2個步驟。 ，其後使其再溶解於水 (c) 相較於後述之電解法，其濃度較 W由於產生電源為高電壓故難故難以溶解。 ,、 1故難以小裀仆。 ⑷於放電之缝水生成裝置中，使㈣ 前需要時間（數分鐘之待機時間） ' 此穩疋 ㈣H 難以—瞬間調製_農度 Θ因^放電型之臭氧氣體產生器並不適合於養殖魚、尤其 疋海序處的利用0 '乍為臭氧水之製法’除了上述放電法以外，已知有由電解 =所進仃之臭氧水製法，相較於放電法，此電解法的電力原 單位雖U ’但因容易得到高漠度之臭氧氣體及水的特徵， 而被通用於電子零件洗淨等之特殊領域中。原理上，由於使 100113338 9 201204417 用直流低壓電源，故瞬時應答性、安全性優越，而可期待作 為小型之臭氧氣體、臭氧水產生器的利用。 為了使臭氧氣體效率佳地產生，不可或缺的是選擇適當的 觸媒與電解質。作為電極材料，已知有鉑等貴金屬、〇!-二氧 化鉛、/?-二氧化鉛、浸含有氟碳之玻璃碳、鑽石。作為電解 質，過去雖利用硫酸、磷酸、含氟基等之水溶液，但操作不 便而未普及。相對於此，如非專利文獻5所記載般，使用固 體高分子電解質作為隔膜、以水作為原料的水電解槽，係管 理容易而通用。若使用習知觸媒之二氧化鉛，則可得到12 重量％以上的高濃度臭氧氣體。 關於使用固體高分子電解質作為隔膜、以原料水作為原料 的水電解槽，專利文獻13中揭示有導電性鑽石可用於作為 機能水（包括臭氧水）用電極。 另外，專利文獻14中，揭示有藉由對電極附近之溶液賦 予充分流速，而在氣體化前以臭氧水之型式取出的方法。 專利文獻15中，提案有溶解臭氧之電解水的喷霧裝置、 尤其是將所得之電解水依霧狀進行喷霧的小型喷霧器裝置。 專利文獻16中，提供一種殺菌淨化裝置，係將所生成之 臭氧水導入至殺菌洗淨槽並藉由超音波作用與臭氧水之殺 菌作用的組合，而發揮殺菌•脫臭之功能。 再者，如非專利文獻6所記載，近年來正進行著有關所謂 奈米氣泡、微氣泡等細微氣泡的基礎性研究或實用化檢討。 100113338 10 201204417 有關最近之發展，已記載於細微氣泡之最新技術中。 尸專利文獻π、ι8中’赫有以⑽氣體為主體的經奈米 氣泡、微氣泡化的含臭氧氣泡’係具有洗淨效果。同技術中， 報告有其對牡糲體内之諾羅病毒的不活化具有效果。 再者’最近’如非專敎獻2所記载般，亦進行著將使氣 化物離子電解氧化而得之電解機能水利用於養魚領域的檢 ‘已報告有依濃度lppm、！分鐘可使魚類病原微生物死 滅，又，對於飼育料，亦可藉由G.5ppm、1G〜3G分鐘之 處理予以消毒。然而’此方法中，有關於作為本發明對象之 養殖魚中所寄生之外部寄生蟲的驅除方法、尤其是如虎 般外皮較厚之魚财所寄生之·㈣蟲、縣吸蟲等外部寄生 蟲的驅除仍屬不明，至此尚未有漏報告。 ° (專利文獻1)日本專利特開2〇〇6_77〇〇〇號公報 (專利文獻2)曰本專利特開2〇〇6 611〇7號公報 (專利文獻3)曰本專利特開2〇〇1_316255號公報 (專利文獻4)曰本專利特開2〇〇2_22〇3〇8號公報 (專利文獻5)曰本專利特開2〇〇8_44862號公報 (專利文獻6)日本專利特公平7_51〇28號公報 (專利文獻7)曰本專利特開平6_467〇8號公報 (專利文獻8)曰本專利特開2〇〇9_5〇215號公報 (專利文獻9)日本專利特開2〇〇〇_1287〇2號公報 (專利文獻10)日本專利特開2〇〇8_142647號公報 100113338 201204417 (專利文獻l1)國際公開第2007/043592號說明書 (專利文獻丨2)曰本專利特開2008-148607號公報 (專利文獻13)日本專利特開平9_268395號公報 (專利文獻14)日本專利特開平〇8_134677號公報 (專利文獻15)日本專利特開2006-346203號公報 (專利文獻16)日本專利特開2〇〇4_357521號公報 (專利文獻Π)曰本專利特開2005-246293號公報 (專利文獻18)日本專利特開2009-226386號公報 (非專利文獻1)吉水守，臭氧年鑑，ρ4〇ΐ-4〇9，1993-1994 年度版 (非專利文獻2)對於魚類病原微生物之殺菌、工業用水， 523，Ρ13-26，平成 14 年 (非專利文獻3)鮭蹲資源管理中心技術情報ρ169，2003 (非專利文獻4)日本醫療臭氧研究會，增刊丨號，1996 (非專利文獻 5)J. Electrochem. Soc.，132, 367，1985 (非專利文獻6)細微氣泡之最新技術，NTS公司，2006 【發明内容】 (發明所欲解決之問題） 習4 σ ’如上述般雖對寄生蟲之驅除方法進行了各種檢討， 但任一方法均無法稱得上能充分解決。 作為養殖魚之驅除方法，較佳係 (a)可依短時間進行寄生蟲 、尤其是體表吸蟲、鰓吸蟲之 100Π3338 12 201204417 驅除； _ (b)可不需耗費調製保管的功夫，於現場安全地進行作業； (c) 可直接將處理水廢棄至海中； (d) 可不對養殖魚生態造成影響而發揮驅除效果。 重要的是提供一種漁業者可實施之具有實效的現場作業 方法。 本發明之目的在於提供一種驅除方法，其係解決上述習知 方法的問題點，可有效驅除寄生於養殖魚中之鰓吸蟲、體表 吸蟲等外部寄生蟲。 (解決問題之手段） - 本發明之第1課題解決手段在於，為了達成上述目的，而 - 於養殖場附近設置電解式臭氧產生裝置，藉由電解式臭氧產 生裝置將原料水進行電解而生成電解臭氧水，將所生成之電 解臭氧水貯藏於電解臭氧水槽内，於該電解臭氧水槽内投入 養殖魚，以驅除寄生於養殖魚之外部寄生蟲。 本發明之第2課題解決手段中，所驅除之外部寄生蟲為鰓 吸蟲或體表吸蟲。 . 本發明之第3課題解決手段中，係使用導電性鑽石電極作 . 為電解式臭氧產生裝置中所使用的電極。 本發明之第4課題解決手段中，係將電解臭氧水槽内之電 解臭氧水溫度設為20〜28°C，將電解臭氧水濃度設為 0.1〜lOppm，將處理時間設為1〜10分鐘之範圍。 100113338 13 201204417 本發明之第5課題解決手段中，係將供給至電解式臭氧產 生裝置之原料水的氣化物離子設為3mM以下。 本發明之第6課題解決手段，係將電解式臭氧產生裝置設 置於船上或港附近之養殖場，並將由上述電解式臭氧產生裝 置所生成之電解臭氧水供給至設置於由養殖區所隔離出之 另一區間的電解臭氧水槽内，於該區間内投入養殖魚，以驅 除寄生於養殖魚之外部寄生蟲。 本發明之第7課題解決手段中，係於上述電解臭氧水槽内 投入由電解式臭氧產生裝置所生成之電解過氧化氫水，使用 上述電解臭氧水與上述電解過氧化氫水之混合水，驅除寄生 於養殖魚的外部寄生蟲。 本發明之第8課題解決手段中，作為養殖魚，係使用虎河 豚等之外皮較厚之魚類的養殖魚。 (發明效果） 藉由本發明，確認到下述效果。 (a) 電解臭氧水可依短時間進行寄生蟲、尤其是體表吸蟲、 鰓吸蟲之驅除； (b) 電解臭氧水可由電能量與水於現場進行合成，不需耗費 調製保管的功夫，可安全地進行作業； (c) 電解臭氧水可迅速地分解為安全的氧，故可直接將處理 水廢棄至海中； (d) 電解臭氧水可不對養殖魚生態造成影響而發揮驅除效 100113338 14 201204417 果。 " ’料’根據本發明’可藉由使用電解臭氧水之簡便方法而 _ 核地驅除寄生於養絲找吸蟲、體表吸鮮外部寄生 蟲。 【實施方式】 以下，根據圖1說明本發明之實施態樣。 〃圖1係表示本發财寄生於養殖魚之㈣蟲或體表吸蟲 等之外部寄生蟲之驅除方法的—實施態樣，丨為電解式臭氧 產生裝置’ 2為電解式臭氧產生裝置之直流電源，3為供給 至電解式臭氧產生裝置之原料水的原料水槽，4為貯藏由電 解式臭氧產生裂置1所生成之臭氧水的電解臭氧水槽。將原 - 料水由原料水槽3供給至電解式臭氧產生裝置丨，並對直流 電源2流通電量時’所供給之原料水於電解式臭氧產生裝置 1内被電解，生成臭氧氣體。此臭氧氣體係溶解於原料水中 而生成既定濃度之電解臭氧水，將所生成之既定濃度之電解 臭氧水供給並貯藏於電解臭氧水槽4内。 其後，將養殖魚投入至電解臭氧水槽4内，維持既定時 ' 間，則於電解臭氧水槽4内將寄生於養殖魚的外部寄生蟲驅 - 除。作為養殖魚，以虎河豚等外皮較厚之魚類的養殖魚最適 合，作為所驅除之外部寄生蟲，為總吸蟲、體表吸蟲。 作為實施本發明之驅除方法的場所’係於沿岸所設置之養 殖區附近，於由該區所隔離出之其他區間中進行。亦即，電 100113338 201204417 解式臭氧產生裝置丨、直流電源2、原料水槽 水槽4均設置於養殖場附近。 及電解臭氧 作為電解式臭氧產生裂置i所使用之陽極 石電極’相較於鉬或二氧化錯電極，為導電鑽 即使於停機時放置仍a 、產生欵率較高、 罝仍T維持活性，故通用性優 電解式臭氧產生4内之驅除，触係設為兮梯 臭氧水溫麟水濃料G 内之 間1〜10分鐘之範圍1於臭氧水之溫度，較佳 =使f時 再循環至電解式臭氧產生裝^，或重新供給氣水 氧而使臭氧錢度轉—定。難練置冷卻裝;^成臭 保持-定。若臭氧水之溫度為此範圍，則養:溫 躍’充分進肋呼吸，促進臭氧水賴的供給^此舌動活 溫7 ’則因溶解氧濃度降低，故對養殖魚而言危險二的 t此範圍⑽可期待藥效。此以上則有對養❹^氧 良’“之虞。又’臭氧氣體之I生量增加，作孝: 效果；若為此==理時間，若為此以下則無法期待 另外“ 養殖魚造成不良影響之虞。 另外，供給至電解式臭氧產生^之原 高臭氧效率，氣化物離子 ’、係為了提 設在_時將一，故在::二：二由於假 =〜化物離子時：量最好為微= 上的魏物離子，則臭氧產生效率降低，⑼代^ 100113338 201204417 成亞氯酸離子。 進而，於電解式臭氧產生農置1附近設置電解式過氧化 產生裝置，於上述電解臭氧水槽4内投入由電解式過氧化气 產生裝置所生成之電解過氧化氫水，使用上述電解臭氧火2 上述電解過氧化氫水之混合水，以驅除寄生於外皮較严之备、 類之養殖魚上的外部寄生蟲，則可有效進行驅除。可認為〜 如上述般使用電解臭氧水與電解過氧化氫水之混合水，則藉 由此等物質及因促進氧化所生成之活性氧種而使寄生蟲表 面被氧化，阻礙正常之生命活動，而誘發從寄生部位的脫 落、驅除。若使用臭氧與過氧化氫之混合水，則因促進氣化 處理而容易生成活性氧。此時之濃度比臭氧：過氧化氫較佳 為1 : 0.1〜1 : 10之範圍。若過氧化氮少於此，則無法期待 促進氧化處理的效果。又，若較此多，則臭氧之分解變快， 僅可得到過氧化氫的效果。 圖2係表示本發明之寄生於養殖魚之總吸蟲或體表吸蟲 等之外部寄生蟲的驅除方法的其他實施態樣，其係於遠離沿 岸之海上養殖場設置養殖用魚場5，於船6之船上設置電解 式臭氧產生裝置1，於船上或船6附近之海上設置電解臭氣 水槽4。 在由養殖魚場5所隔離出之另一區間中設置電解臭氧水 槽4，於此槽4内注入臭氧水，將養殖魚場中之魚移送至該 區間申，經處理後，再將魚流放至原本的魚場5中。作為處 100113338 17 201204417 理區間，可為合成、搬運臭氧水時所使用的容器。為了防止 臭氧水釋出臭氧氣體，較佳為具有蓋的區間、容器。與海水 所分離區分出之區間的水量，係依存於魚類與重量，為 1〜10m左右。有效的是重複數次之上述驅除方法。 臭氧水係於船上或漁港中，由裝滿原料水之容器將原料水 輸送至電解槽，以合成臭氧水。 針對本發明所使用之電解式臭氧產生裝置之一例進行詳 述0 (1)電解式臭氧產生裝置 圖3表示電解式臭氧產生槽之一例。圖3中，7為陽極， 8為陰極’ 9為離子交換膜，電解式臭氧產生槽係由離子交 換膜9’區分為含有陽極7之陽極室與含有陰極8之陰極室。 電極間距離較佳為G lmm〜5Gmme若較此距離近，則容 易因接觸㈣生⑯路，若較此距離遠，料至槽電壓增加。 電極間距離更佳為Q lmmJ_ 2rnm左右。各電極室中，設有 原料水之供給口與排出口、生成氣體之排出口。所合成之電 解臭氧水村保麵電極㈣，祕健存於 ㈣題， (2)電極反應 ’藉由電解而 電解槽中之陽極反應，係供給屬制料之水 進行 100113338 201204417 2H20=〇2+4H++4e' 的氧產生，但視觸媒、電解條件而生成 3H20=〇3H-6H++6e' 之臭氧，可合成出使其溶解的臭氧水。 (3)陽極材料 作為陽極7之陽極基材，限定為鈦、鈮等之閥金屬（Vdve201204417 VI. Description of the Invention: [Technical Field to Be Invented by the Invention] The present invention relates to a method for repelling an external parasite such as schistosomiasis and surface fluke which is parasitic on farmed fish. [Prior Art] In recent years, marine fish farming technology has developed, and high-grade fish such as green sorghum, lionfish, flounder, scorpionfish, and puffer fish have become thinner objects. In the thinning field, in order to shorten the breeding period and increase the value of the commodity, it is important to prevent diseases caused by viruses, pathogenic bacteria and external parasites. However, in the period from May to August (water temperature 22 ° C to 27 ° C) which is suitable for the temperature of the external parasite to proliferate, a large number of deaths of farmed fish often occur. Even if the degree of death is not reached, the cultured fish are reduced in physical strength due to parasites, and the food is poor, and the value of the commodity is lost. It is generally cited that after the use of the organotin-based anti-algae agent, an effective repelling method has not been developed for the increase in parasite damage. However, although a commercially available pharmaceutical preparation for mixing fish in a fish-fed blended feed and oral administration has been widely used, it has been confirmed only for a part of parasites. As a representative of the parasite, there are two kinds of parasitic worms, such as the surface parasitic body and the fish mites. As a method of repelling parasites in farms, the following methods have been reviewed so far. (1) Freshwater bath method, concentrated brine bath method The so-called fresh water bath method and the concentrated brine bath method mean that the fish is subjected to impregnation treatment in fresh water or concentrated brine prepared in a ship tank or the like for several minutes to cause parasitic The insects weaken due to changes in osmotic pressure and fall off from the fish body and die. Although this method affects the fish body due to the difference in salt concentration of water treated with 100113338 4 201204417, it must be transported to the farm where it is implemented, but it is an appropriate method because there is no need to worry about seawater pollution. (2) medicated bath method The medicated bath method is to remove the parasite from the fish body by using the action of peroxidic sodium pyrophosphate, sodium percarbonate, peroxyacid disodium, formaldehyde, glacial acetic acid and the like. However, the drug has the problem of polluting the surrounding seawater. As a medicated bath method, the following method is conventionally carried out. Patent Document 1 discloses that parasites are repelled by fish infected with parasites such as Fasciola by ferulic acid and lactic acid. Patent Document 2 discloses a method for suppressing and preventing parasitic diseases of mariculture fish by administering a parasite inhibitor containing a cocoa bean composition as an active ingredient. Patent Document 3 discloses a method of adding δ-aminolevulinic acid to a feed or a water tank to treat fish infected with pathogenic microorganisms and parasites. Patent Document 4 discloses a use of a benzimidazole-based drug as an active ingredient as a repellent and a repellent method for parasitic in the total absorption of tiger pufferfish. Patent Document 5 discloses a method of using a stabilized chlorine dioxide or chlorite, an organic carboxylic acid, and hydrogen peroxide to treat and prevent fish scutellaria ciliate infection (Scuticociliatosis). Shield-like cilia has a deeper intrusion into the body, so it is different from other parasitic diseases. It is well known that furfural, which has been conventionally used, has been completely banned from being used in farmed fish. 100113338 5 201204417 (3) Hydrogen peroxide method There is also a method of using hydrogen peroxide as a method for deworming parasites or a method for preventing infection. Patent Document 6 is a method for providing a parasite that is easy to operate without any conventional method, and that is easy to handle and can be efficiently processed even in a large amount of processing to drive out parasites. There is a method of suturing the culture of the marine fish farm in the fish swimming area of the fish, and diluting it to a suitable concentration of hydrogen peroxide solution (4). In Patent Document 6, the preferred range is preferably selected within the range of a hydrogen peroxide aqueous solution concentration of 400 to 1000 ppm and a treatment time of 3 to 10 minutes. = However, it is quite dangerous to transfer a thick liquid of hydrogen peroxide solution. Moreover, if hydrogen peroxide is used frequently, it will enhance the parasite's financial acceptability to hydrogen peroxide. In Patent Document 7, as a method for preventing Heterobothrium (S. cerevisiae) in a tiger puffer fish in a marine farm, it is disclosed that the concentration of the peroxidizing gas is 400 ppm or more and the phytotoxicity is not caused to the fish body, and the treatment is performed. The fish body is treated under the condition of more than 20 minutes, and the worms parasitized at the stage of cockroach can be driven away. Patent Document 8 discloses that hydrogen peroxide is added to a hydrogen peroxide concentration of 10 to 600 ppm, and the irradiation intensity of the water surface of the above-mentioned blocked water system is 2 to 3 〇mW/cm 2 while maintaining the above concentration. 240~370nm UV for 3~40 minutes to kill the parasite eggs of farmed fish. Further, in Patent Document 9, peracetic acid is used as an active ingredient, and after adding 100113338 6 201204417 cerium oxide to a concentration of 8 〇 ppm to 2000 ppm, and then neutralizing, the cultured fish is immersed in the treatment liquid. Treatment, in order to avoid damage to farmed fish in a wide temperature range, and try to remove parasites from farmed fish. (4) Other methods As other methods, Patent Documents 10 and U disclose that there is a kind of photocatalyst reaction to aquatic money, which is caused by the diffusion of the oxygen species generated by the light-contacting medium by the _ sub-water test. (4) The function of water _ active oxygen species, the oxidation of anti-money, and the repelling of the insects and protozoa of microorganisms. Money, for the death of the 14β insect, must be utilized by the parasitic patent ^ nutrient by an external line. In Patent Document 12, in order to suppress the occurrence of dying in aquaculture, the fish are protected from parasitic diseases caused by parasitic diseases (10), and the village is exposed, especially in the stage of fish feeding. Practicality. ", and vaccines are used, but it is still a study. (5) Method for performing ozone water Non-patent document 1 towel, which describes a bactericidal effect belonging to oxidized microorganisms. Ozone for fish pathogen Non-patent document 2 discloses ozone water treatment In the concentration of 〇.3~0.5ppmT5~1〇, combined with the water for breeding, and the equipment in the tank, in order to avoid the interaction with the desertification in the seawater: there is effect. However, the effect of feeding the fish must be reduced. The indifference caused by the reaction to Hundreds of Eight is reported in Non-Patent Document 3. (4) 100113338 'The treatment of ppm. 3ppm, 5 minutes 201204417 can prevent cerebral larvae (Ceratomyxa), Myxozoa genus Examples of parasitic infections. However, in the case where bromide ions are present in seawater, since they react with ozone to form residual and harmful bromate ions, it is preferable to remove them in advance. Among the methods carried out by ozone water, there is a method for repelling the external parasites parasitized in the cultured fish which are the object of the present invention, especially the fish which are thicker than the outer skin of the tiger porpoise. The repelling of external parasites such as worms and surface flukes is still unknown. Ozone water for ozone gas has been solved. & FDA (Food and Drug Administration) is registered in the food supplement table. It has been approved as a fungicide in food storage and manufacturing steps (2GG1 years), and has many practical achievements in killing g in food 4 and sterilization of food itself. The advantages of ozone water are as follows: (a) Ozone ( The bactericidal effect by the OH radical is the oxidative destruction of the cell wall and there is no difference, so there is no resistant bacteria. (b) Since it is decomposed into oxygen, there is no harmful secondary product. (c) No residue. Sexuality is both an advantage and a disadvantage. If the ozone gas can be stably stored in a solution, it can be expected to be applied and the effect is expanded. In addition, there is a long history of ozone therapy for human body, and it is a good way to treat it with beneficial methods. Recently, as described in Non-Patent Document 4, the use of livestock and pets has progressed 100113338 201204417. Recent research on oxygenation will inactivate the immune system. For breeding months, the stench energy that enters the bloodstream Ozone water, which has been shown to have such an effect, has been commonly used since its inception, and can be easily manufactured to produce a ppm of ozone gas generators in the field of ozone water and detergent cleaning. In the case of clean water treatment and smashing, there are restrictions on the use of the gas. The gas generator is based on the following (a) If dry air is used as the raw material, a to a high concentration gas must be produced using pure oxygen raw materials. N〇x. In order to obtain (b) there must be two steps to make ozone first in gas form. Then, it is redissolved in water (c). Compared with the electrolysis method described later, the concentration is higher than W. The power supply is high voltage, so it is difficult to dissolve. , 1, 1 is difficult to be a small servant. (4) In the discharge water generating device for discharge, it takes time (a few minutes of standby time) before making (4) ' This stable (four) H is difficult - instantaneous modulation _ agricultural degree Θ due to the discharge type ozone gas generator is not suitable for farmed fish, In particular, the method of using 0 '乍 as ozone water in the sea order is known. In addition to the above-mentioned discharge method, there is known a method of producing ozone water by electrolysis = compared with the discharge method, although the electric power unit of the electrolysis method is U 'But it is widely used in special fields such as electronic parts cleaning because it is easy to obtain the characteristics of high-intensity ozone gas and water. In principle, since 100113338 9 201204417 is used with a DC low-voltage power supply, it is excellent in transient response and safety, and can be expected to be used as a small ozone gas or ozone water generator. In order to produce ozone gas efficiently, it is indispensable to select an appropriate catalyst and electrolyte. As the electrode material, a noble metal such as platinum, bismuth--lead dioxide, /?-lead dioxide, glass carbon immersed in fluorocarbon, and diamond are known. As the electrolyte, an aqueous solution of sulfuric acid, phosphoric acid, or a fluorine-containing group has been used in the past, but it has not been widely used because of its inconvenience. On the other hand, as described in Non-Patent Document 5, a water-based electrolytic cell using a solid polymer electrolyte as a separator and water as a raw material is easy to handle and is common. When a conventional catalyst of lead dioxide is used, a high concentration ozone gas of 12% by weight or more can be obtained. In the water electrolysis cell using a solid polymer electrolyte as a separator and raw material water as a raw material, Patent Document 13 discloses that a conductive diamond can be used as an electrode for functional water (including ozone water). Further, Patent Document 14 discloses a method of taking out a pattern of ozone water before gasification by giving a sufficient flow rate to a solution in the vicinity of the electrode. Patent Document 15 proposes a spray device for dissolving dissolved water of ozone, in particular, a small spray device for spraying the obtained electrolyzed water in a mist form. Patent Document 16 provides a sterilizing and purifying apparatus which introduces the generated ozone water into a sterilizing washing tank and combines the action of ultrasonic waves with the bactericidal action of ozone water to exhibit a function of sterilization and deodorization. Further, as described in Non-Patent Document 6, basic research or practical review of fine bubbles such as nanobubbles and microbubbles has been conducted in recent years. 100113338 10 201204417 Recent developments have been documented in the latest technology in microbubbles. The corpus patent documents π and ι8 have a cleaning effect by a nano-bubble or a microbubble-containing ozone-containing bubble mainly composed of a (10) gas. In the same technology, it has been reported to have an effect on the inactivation of norovirus in oysters. In addition, as for the "recent", it is also reported that the electrolyzed functional water used for electrolytic oxidation of gas ions is used in the field of fish farming. Minutes can kill fish pathogenic microorganisms, and for breeding materials, it can be disinfected by G.5ppm, 1G~3G minutes. However, in this method, there is a method of repelling the parasitic external parasites in the cultured fish which is the object of the present invention, especially the fish parasites which are thicker than the tiger skin. (4) External parasites such as insects and trematodes The expulsion is still unclear, and no reports have been reported so far. (Patent Document 1) Japanese Patent Laid-Open Publication No. Hei. No. Hei. No. Hei. No. 2, No. 6, No. 6,611, No. 2 (Patent Document 3) Japanese Laid-Open Patent Publication No. Hei 2 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ Japanese Laid-Open Patent Publication No. Hei. No. Hei. No. Hei. No. Hei. No. Hei. Japanese Patent Laid-Open Publication No. Hei. No. Hei. No. Hei. No. Hei. No. Hei. No. Hei. No. Hei. No. Hei. No. Hei. Japanese Laid-Open Patent Publication No. Hei. No. Hei. No. Hei. No. Hei. No. Hei. No. Hei. No. Hei. No. Hei. No. Hei. 2〇〇4_357521 (Patent Document Π) 曰本专利特开2005-246293号Japanese Patent Laid-Open Publication No. 2009-226386 (Non-Patent Document 1) Ji Shoushou, Ozone Yearbook, ρ4〇ΐ-4〇9, 1993-1994 Edition (Non-Patent Document 2) for fish pathogenic microorganisms Sterilization, industrial water, 523, Ρ13-26, Heisei 14 (Non-Patent Document 3) 鲑蹲 Resource Management Center Technical Information ρ169, 2003 (Non-Patent Document 4) Japan Medical Ozone Research Association, Supplement nickname, 1996 (Non- Patent Document 5) J. Electrochem. Soc., 132, 367, 1985 (Non-Patent Document 6) The latest technology of fine bubbles, NTS Corporation, 2006 [Summary of the Invention] (The problem to be solved by the invention) Although various methods have been reviewed for the method of repelling parasites, neither method can be adequately solved. As a method for expelling farmed fish, it is preferred that (a) can be repelled in a short period of time by parasites, especially surface flukes and ticks, 100 Π 3338 12 201204417; _ (b) can be used without modulating and storing Work safely on site; (c) Dispose of treated water directly into the sea; (d) Explode without affecting the ecology of farmed fish. It is important to provide a field work method that can be implemented by a fisherman. SUMMARY OF THE INVENTION An object of the present invention is to provide a method for repelling which solves the problems of the above-mentioned conventional methods and can effectively eliminate external parasites such as ticks and surface fluke which are parasitic in farmed fish. (Means for Solving the Problem) - In order to achieve the above object, the first object of the present invention is to provide an electrolytic ozone generating device in the vicinity of a farm, and to electrolyze raw material water to generate electrolysis by an electrolytic ozone generating device. Ozone water, the generated electrolytic ozone water is stored in an electrolytic ozone water tank, and the cultured fish is put into the electrolytic ozone water tank to drive out the external parasites parasitic on the cultured fish. In the second aspect of the present invention, the external parasite to be repelled is ticks or surface flukes. In the third aspect of the present invention, a conductive diamond electrode is used as an electrode used in an electrolytic ozone generating device. In the fourth aspect of the present invention, the temperature of the electrolytic ozone water in the electrolytic ozone water tank is set to 20 to 28 ° C, the electrolytic ozone water concentration is set to 0.1 to 10 ppm, and the treatment time is set to 1 to 10 minutes. range. In the solution of the fifth aspect of the present invention, the vaporization ions of the raw material water supplied to the electrolytic ozone generator are set to 3 mM or less. According to a sixth aspect of the present invention, an electrolytic ozone generating device is installed on a ship or a farm near a port, and the electrolytic ozone water generated by the electrolytic ozone generating device is supplied to be disposed in a culture area. In another section of the electrolytic ozone tank, farmed fish are placed in this section to drive out parasites that are parasitic on farmed fish. According to a seventh aspect of the present invention, an electrolytic hydrogen peroxide water produced by an electrolytic ozone generating device is introduced into the electrolytic ozone water tank, and the mixed water of the electrolytic ozone water and the electrolytic hydrogen peroxide water is used to drive off An external parasite that is parasitic on farmed fish. In the eighth aspect of the present invention, a farmed fish is a farmed fish of a fish having a thick outer skin such as a tiger puffer. (Effect of the Invention) According to the present invention, the following effects are confirmed. (a) Electrolyzed ozone water can be used to remove parasites, especially surface flukes and ticks in a short period of time; (b) Electrolyzed ozone water can be synthesized on site by electric energy and water, without the need to modulate and store (c) Electrolyzed ozone water can be quickly decomposed into safe oxygen, so the treated water can be directly discarded into the sea; (d) Electrolyzed ozone water can exert the effect of repelling without affecting the culture of farmed fish 100113338 14 201204417 fruit. "Materials' can be used to remove parasitic larvae from the filatures and to absorb external parasites by using a simple method of electrolyzing ozone water. [Embodiment] Hereinafter, an embodiment of the present invention will be described with reference to Fig. 1 . Figure 1 shows an embodiment of the method for repelling external parasites such as (4) insects or surface flukes in cultured fish, and the electrolytic ozone generating device is an electrolytic ozone generating device. The DC power source 3 is a raw material water tank supplied to the raw material water of the electrolytic ozone generating device, and 4 is an electrolytic ozone water tank for storing ozone water generated by the electrolytic ozone generating crack 1 . The raw material water is supplied from the raw material water tank 3 to the electrolytic ozone generating device 丨, and the raw material water supplied to the direct current power supply 2 is electrolyzed in the electrolytic ozone generating device 1 to generate ozone gas. This ozone gas system is dissolved in the raw material water to generate electrolytic ozone water of a predetermined concentration, and the generated electrolytic ozone water of a predetermined concentration is supplied and stored in the electrolytic ozone water tank 4. Thereafter, the cultured fish is put into the electrolyzed ozone tank 4, and while maintaining the timing, the external parasites parasitic on the cultured fish are driven out in the electrolytic ozone tank 4. As a farmed fish, the fish cultured with thicker skins such as tiger puffer fish is most suitable, and the external parasite that is driven out is the total trematode and surface fluke. The place where the repelling method of the present invention is carried out is carried out in the vicinity of the cultivating area provided along the coast, and is carried out in other sections separated by the area. That is, the electricity 100113338 201204417 solution ozone generating device 丨, DC power source 2, raw material sink sink 4 are all located near the farm. And the electrolyzed ozone as the anode electrode used in the electrolytic ozone generation cracking i is compared with the molybdenum or the dioxing electrode, so that the conductive drill is placed even when it is stopped, the yield is high, and the crucible is still active. Therefore, the general-purpose electrolysis type ozone generation 4 is driven out, and the contact system is set to the temperature of the ozone water in the range of 1 to 10 minutes between the temperature of the ozone water and the temperature of the water. Recycling to the electrolytic ozone generating device, or re-supply the gas water oxygen to make the ozone money turn. Difficult to set the cooling equipment; ^ into the smell to maintain - fixed. If the temperature of the ozone water is in this range, then raise: warmth ‘full rib breathing, promote the supply of ozone water ^ ^ this tongue moving temperature 7 ' is due to the dissolved oxygen concentration is reduced, it is dangerous for farmed fish t This range (10) can be expected to be effective. The above is the 对 ❹ 氧 氧 氧 氧 氧 氧 又 又 又 又 又 又 臭氧 臭氧 臭氧 臭氧 臭氧 臭氧 臭氧 臭氧 臭氧 臭氧 臭氧 臭氧 臭氧 臭氧 臭氧 臭氧 臭氧 臭氧 臭氧 臭氧 臭氧 臭氧 臭氧 臭氧 臭氧 臭氧 臭氧 臭氧 臭氧 臭氧 臭氧 臭氧 臭氧 臭氧The impact of adverse effects. In addition, the supply of high-efficiency ozone to the electrolytic ozone generation, the vaporization ion ', will be one in order to be set at _, so in:: two: two due to false = ~ compound ions: the amount is preferably micro = on the Weifang ion, the ozone production efficiency is reduced, (9) generation ^ 100113338 201204417 into chlorite ion. Further, an electrolytic peroxidation generating device is provided in the vicinity of the electrolytic ozone generating agricultural plant 1, and the electrolytic hydrogen peroxide water generated by the electrolytic peroxidizing gas generating device is introduced into the electrolytic ozone water tank 4, and the electrolytic ozone fire is used. The mixed water of the above-mentioned electrolyzed hydrogen peroxide water can be effectively repelled by repelling external parasites which are parasitic on the cultured fish of the outer skin. It is considered that when the mixed water of electrolytic ozone water and electrolytic hydrogen peroxide water is used as described above, the surface of the parasite is oxidized by such substances and the active oxygen species generated by the promotion of oxidation, thereby hindering normal life activities. It is induced to fall off and repel from the parasitic part. When mixed water of ozone and hydrogen peroxide is used, active oxygen is easily generated by promoting gasification treatment. The concentration at this time is more than ozone: hydrogen peroxide is preferably in the range of 1:0.1 to 1:10. If the amount of nitrogen peroxide is less than this, the effect of promoting the oxidation treatment cannot be expected. Further, if it is more than this, the decomposition of ozone becomes fast, and only the effect of hydrogen peroxide can be obtained. Fig. 2 is a view showing another embodiment of the method for repelling the external parasites of the total trematode or the surface flukes of the cultured fish of the present invention, which is provided for the breeding fish farm 5 at a marine farm far from the coast, An electrolytic ozone generating device 1 is provided on the ship of the ship 6, and an electrolytic odor water tank 4 is provided on the ship or near the sea of the ship 6. In another section separated by the farmed fish farm 5, an electrolyzed ozone tank 4 is arranged, and ozone water is injected into the tank 4, and the fish in the farmed fish farm is transferred to the section, and after being processed, the fish is discharged. To the original fish farm 5 . As a container, it can be used as a container for synthesizing and transporting ozone water. In order to prevent ozone water from releasing ozone gas, it is preferable to have a cover section and a container. The amount of water separated from the seawater depends on the fish and the weight, and is about 1 to 10 m. It is effective to repeat the above-described repelling method several times. The ozone water is supplied to the vessel or the fishing port, and the raw material water is sent to the electrolytic tank by a container filled with raw water to synthesize ozone water. DETAILED DESCRIPTION OF THE INVENTION An example of an electrolytic ozone generating apparatus used in the present invention is as follows: (1) Electrolytic ozone generating apparatus Fig. 3 shows an example of an electrolytic ozone generating tank. In Fig. 3, 7 is an anode, 8 is a cathode, and 9 is an ion exchange membrane. The electrolytic ozone generating tank is divided by an ion exchange membrane 9' into an anode chamber containing an anode 7 and a cathode chamber containing a cathode 8. The distance between the electrodes is preferably G lmm~5Gmme. If it is closer than this distance, it is easy to contact 16 holes due to contact (4). If it is farther away from this distance, the material-to-slot voltage increases. The distance between the electrodes is preferably about Q lmmJ_ 2rnm. In each of the electrode chambers, a supply port and a discharge port for the raw material water and a discharge port for generating a gas are provided. The electrolyzed ozone water reserving surface electrode (4) synthesized by the company, the secret health is stored in (4), (2) the electrode reaction 'the anode reaction in the electrolysis cell by electrolysis, and the water supplied to the material is 100113338 201204417 2H20=〇2+ Oxygen is generated by 4H++4e', but ozone of 3H20=〇3H-6H++6e' is generated depending on the catalyst and electrolysis conditions, and ozone water which is dissolved can be synthesized. (3) Anode material As the anode substrate of the anode 7, it is limited to a valve metal such as titanium or tantalum (Vdve)
Metals)、其合金、矽。作為觸媒，可利用鉑、鑽石、二氧 化錯。 鑽石由於可藉由摻雜而控制電傳導性，故可期待作為電極 材料。鑽石電極已報告有其對水分解反應呈惰性，藉由氧化 反應，除了氧以外，尚生成臭氧、過氧化氫。觸媒可存在於 陽極之一部分，亦可使上述基材之一部分露出無妨。以下說 明代表性之熱燈絲CVD法。使用成為碳源之曱烷CH4等碳 化氫氣體、或醇等有機物，與氫氣一起送入至CVD室内， 一邊保持還原環境’ 一邊加熱燈絲，使其成為生成碳自由基 之溫度1800〜2400X：。此時，於析出鑽石之溫度(750〜950。〇 區域設置電極基材。碳化氫氣體相對於氫的濃度為 0.1~10vol%，壓力為 2〇hPa〜WUhPaG 大氣壓）。 鑽石係為了得到良好之導電性，必須微量添加原子價不同 之元素。硼B或磷p之較佳含有率為卜i〇〇〇〇〇ppnl，更佳 為100〜lOOOOppm。原料化合物係使用三曱基硼（CH3)3B，但 較佳係利用毒素少的氧化硼b2o3、五氧化二磷P205等。作 100113338 19 201204417 為電極基材之形狀，可為粒子、纖維、板、 (4)陰極材料 双棒專。 陰極8之陰極反應主要為產生鼓，較佳為對氣不 的電極觸媒，較佳有如翻族金屬、錄、不録鋼、欽、生& 銀、碳、鑽石等。作為陰極8之陰極基材，限定' 錯、金、 鈦等。本發明之裝置中，由於屬，:::有 =或過氧化物之水接觸的配置，故叫氧化性優越者為 (5)膜材料 為了穩定保持由電極反應所生成之活性物質，可利 ㈣或離子交換膜。膜可為氟樹脂系、碳化氫樹脂一 種’由臭氧或過氧化物耐純方面而言 為傕用雜；a 佳為別者。圖3 用離子讀膜9之例，離子交賴9 _止陽極7、陰 極8所生叙物龍相反之電極所雜 - 電導度較低的情況下仍使電解迅 〃有即使在液體 乏傳導性之原料水等作為原料的卜仃、機能’故在利用缺 佳為氟樹脂系、聚醯亞胺樹月旨系Μ為必須。作為材質，較 (6)原料水與電解條件 自來水、井水為可·之原料水 之原料水，有於陰極表面沉搬氫。又，含有較多金屬離子 應之虞。又，於陽極表面析二化物或碳酸化物而妨礙反 止此情形，藉由每隔適當時氣化石夕等之氧化物。為了防 100113338 分鐘至1小時)賦予逆電 20 201204417 μ，於陰極進打酸性化’於陽極進行驗化，貝q因產生氣體及 . 供給水之流動而予以加速，使析出物之脫離反應容易進行。 . 軸電極密度越大、臭氧之電流料越增加，但因藉發熱 所進订之分解亦被促進，故適當範圍較佳設為 0.05〜5A/cm2。由於溫度越低、電極中之臭氧之電流效率越 增加’且因溶解度亦越增加，故較佳，但因槽電壓亦屬增加 要因，故溶液溫度較佳為5ΐ〜3〇t。為了提高臭氧效率， 氣化物離子濃度較佳設為3 mM以下。 若使用臭氧與過氧化氣之混合水，則目促進氧化處理而容 易生成活性氧。視目的推薦予以混合使用。此時之濃度比 -(〇3 . H2〇2)較佳為1 : 0_1〜1 : 10之範圍。亦可藉由電解合 - 成過氧化氫。 (實施例） 接著說明本發明之實施例，但本發明並不限定於此等。 [實施例1] 使用形成有導電性鑽石之親製板狀開孔電極(5cmx2〇cm) 作為陽極7。夾住杜邦製Nafi〇n臈(杜邦之註冊商標)作為離 .子交換膜9 ’並使用藉鍍敷形成了 _ G 2微米的鈦製板狀電 . 極(5cmx2〇Cm)作為陰極8。建構成安装了 2〇片此等電極_ 膜接合體之具有陽極室、&極室之2室的電解槽。整體之電 極投影面積為〇.2m2。電解槽之各室中設有氣體、液體之流 路。以原料水作為原料，由電解室下部依每分鐘供給500L。 100113338 21 201204417 依25A/dm2流通電流，將溫度於出口控制為25〇c。所合成 出之臭氧水濃度為2ppm。分出其一部分進行以下試驗。寄 生蟲之生死確認，係先以肉眼確認有無動作，再藉顯微鏡4 細確認，進而視需要重放於海水中確認有無復活。 由體長15cm之虎河豚之鰓所切除之總吸蟲 (Heterobothrium okamotoi，本體4〜5mm長），係依由有機質 之保s蒦皮膜所被覆的狀態’接觸至臭氧水時呈白丨蜀，經1 分鐘處理後，確認其死滅。 對寄生於鰓部位之狀態的鰓吸蟲及鰓肉片以相同臭氧水 進行處理’結果於10分鐘後確認到藥效。 將鰓吸蟲所寄生之河豚置入相同臭氧水中丨分鐘後，重放 至海水中。其體力回復，飼料之攝取狀態亦良好。其後予以 解剖，確認有無鰓吸蟲，但並未檢測到生存之總吸森。 [比較例1] 將由實施例1所使用之體長15cm之虎河豚之總所切除的 鰓吸蟲’藉過氧化氫水(600ppm)進行處理，結果在鰓吸蟲死 滅前需要5分鐘。尚且，藉此過氧化氫水(6〇〇ppm)對寄生於 虎河豚之鰓部位之狀態的鰓吸蟲及鰓肉片進行虑 s W 土苑果麵 吸蟲及無肉片於20分鐘時亦無法死滅。寄生有鰓吸義之、、可 豚未能恢復體力。經解剖後，確認到鰓吸蟲的生存。 上述實施例中，雖針對虎河豚等外皮較厚之養殖魚進行纪 載，但本發明並不限定於此等，亦可應用於其他養殖魚。又， 100113338 22 201204417 作為外部寄生蟲，亦可應用於鰓吸蟲、體表吸蟲以外之外部 寄生蟲。 (產業上之可利用性） 本發明可於養殖場中，廣泛地利用作為預防寄生於養殖魚 之鰓吸蟲、體表吸蟲等之外部寄生蟲所造成之疾病的方法， 並可利用於縮短養殖期間、提升商品價值的領域。 【圖式簡單說明】 圖1為表示本發明一實施態樣的圖。 圖2為表示本發明其他實施態樣的圖。 圖3為表示本發明所使用之電解式臭氧產生裝置之一例 的圖。 【主要元件符號說明】 1 電解式臭氧產生裝置 2 直流電源 3 原料水槽 4 電解臭氧水槽 5 養殖魚場 6 船 7 陽極 8 陰極 9 離子交換膜 100113338 23Metals), its alloys, niobium. As the catalyst, platinum, diamond, and dioxin can be used. Since diamond can be controlled by doping to control electrical conductivity, it can be expected as an electrode material. Diamond electrodes have been reported to be inert to water decomposition reactions. Oxidation reactions, in addition to oxygen, produce ozone and hydrogen peroxide. The catalyst may be present in one of the anodes and may also expose a portion of the substrate. A representative hot filament CVD method will be described below. A hydrocarbon gas such as decane CH4 which is a carbon source or an organic substance such as an alcohol is supplied to the CVD chamber together with hydrogen gas, and the filament is heated while maintaining the reducing atmosphere to a temperature of 1800 to 2400X which generates carbon radicals. At this time, the temperature of the precipitated diamond (750 to 950. The electrode substrate is provided in the region. The concentration of the hydrocarbon gas with respect to hydrogen is 0.1 to 10 vol%, and the pressure is 2 〇 hPa to WUHPaG atm). In order to obtain good conductivity, diamonds must be added with trace amounts of different elements. The preferred content of boron B or phosphorus p is i i pp nl, more preferably 100 to 100 ppm. As the raw material compound, trimethylboron boron (CH3) 3B is used, but boron b2O3, phosphorus pentoxide P205 or the like having less toxin is preferably used. 100113338 19 201204417 The shape of the electrode substrate can be particles, fibers, plates, and (4) cathode materials. The cathode reaction of the cathode 8 is mainly to produce a drum, preferably an electrode catalyst for gas, preferably such as a turn-over metal, recorded, unrecorded steel, chin, raw & silver, carbon, diamond, and the like. As the cathode substrate of the cathode 8, "error, gold, titanium, etc." are defined. In the device of the present invention, due to the genus::: having a configuration in which the water of the peroxide or the peroxide is contacted, it is called that the oxidizing property is superior (5) the membrane material is stable in order to stably maintain the active material generated by the electrode reaction. (d) or ion exchange membrane. The film may be a fluororesin type or a hydrocarbon resin. 'The ozone or peroxide is used for the purpose of purity resistance; a is preferable. Fig. 3 In the case of ion-reading film 9, the ion exchange 9 _ anode 7 and the cathode 8 are the opposite of the electrode of the syllabary dragon - the conductivity is low, and the electrolysis is fast even if the liquid is depleted. It is necessary to use the fluororesin and the polyimine tree as the raw material, such as raw material water and the like. As a material, (6) Raw material water and electrolysis conditions Tap water and well water are the raw material water of the raw material water, and hydrogen is deposited on the surface of the cathode. Also, it contains a lot of metal ions. Further, the formation of a compound or a carbonate on the surface of the anode hinders the reaction, and the oxide of the stone or the like is gasified at every appropriate time. In order to prevent 100,113,338 minutes to 1 hour), the reverse electricity 20 201204417 μ is applied, and the acid is acidified at the cathode to be tested at the anode, and the shellfish q is accelerated by the flow of the generated gas and the feed water, so that the precipitate is easily separated. get on. The higher the density of the shaft electrode, the more the current of the ozone is increased, but the decomposition by the heat is also promoted, so the appropriate range is preferably set to 0.05 to 5 A/cm2. Since the lower the temperature, the more efficient the current of the ozone in the electrode is, and the solubility is increased, it is preferable, but since the cell voltage is also an important factor, the solution temperature is preferably 5 ΐ to 3 〇t. In order to increase ozone efficiency, the vapor ion concentration is preferably set to 3 mM or less. When a mixed water of ozone and peroxidation gas is used, the oxidation treatment is promoted to easily generate active oxygen. It is recommended to be mixed according to the purpose. The concentration ratio -(〇3.H2〇2) at this time is preferably in the range of 1:0_1 to 1:10. It can also be hydrogenated by electrolysis. (Embodiment) Next, an embodiment of the present invention will be described, but the present invention is not limited thereto. [Example 1] A pre-made plate-shaped aperture electrode (5 cm x 2 〇 cm) in which a conductive diamond was formed was used as the anode 7. Nafi〇n臈 (registered trademark of DuPont) was used as the separator 8 and a titanium plate-shaped electrode (5 cm x 2 〇 Cm) of _G 2 μm was formed by using plating. An electrolytic cell having two chambers of the electrode assembly and the two chambers of the anode chamber and the chamber was mounted. The overall electrode projection area is 〇.2m2. A gas and liquid flow path is provided in each chamber of the electrolytic cell. Raw material water was used as a raw material, and 500 L was supplied per minute from the lower portion of the electrolysis chamber. 100113338 21 201204417 According to the current flowing through 25A/dm2, the temperature is controlled at the outlet to 25〇c. The concentration of ozone water synthesized was 2 ppm. Part of it was divided into the following tests. Confirmation of the life and death of the parasitic insects is first confirmed with the naked eye, and then confirmed by the microscope 4, and then replayed in the seawater as needed to confirm the resurrection. The total trematode (Heterobothrium okamotoi, body 4 to 5 mm long) excised from the larvae of the tiger pufferfish, which is 15 cm in length, is covered by the membrane of the organic matter, and is white when exposed to ozone water. After 1 minute of treatment, confirm its death. The schistosomiasis and the mashed meat pieces in the state of parasitic sputum were treated with the same ozone water. The results were confirmed after 10 minutes. The pufferfish parasitized by the mites were placed in the same ozone water for a minute and then replayed into the seawater. The physical strength is restored and the state of intake of the feed is also good. Thereafter, it was dissected to confirm the presence or absence of ticks, but the total absorption of survival was not detected. [Comparative Example 1] The schistosomiasis excised from the total of the tiger pufferfish having a body length of 15 cm, which was used in Example 1, was treated with hydrogen peroxide water (600 ppm), and it was required to take 5 minutes before the cockroach died. Furthermore, it is not possible to use the hydrogen peroxide water (6 〇〇ppm) for the worms and cockroaches that are parasitic on the scorpion of the tiger pufferfish. Dead. Parasitic sputum has the meaning of sucking, but the dolphins can not recover their strength. After dissection, the survival of the ticks was confirmed. In the above embodiment, the cultured fish having a thick outer skin such as tiger puffer fish is described, but the present invention is not limited thereto and can be applied to other farmed fish. Further, 100113338 22 201204417 As an external parasite, it can also be applied to external parasites other than ticks and surface flukes. (Industrial Applicability) The present invention can be widely used as a method for preventing diseases caused by external parasites such as schistosomiasis and surface fluke which are parasitic on farmed fish, and can be utilized in a farm. Shorten the field of cultivation and increase the value of goods. BRIEF DESCRIPTION OF THE DRAWINGS Fig. 1 is a view showing an embodiment of the present invention. Fig. 2 is a view showing another embodiment of the present invention. Fig. 3 is a view showing an example of an electrolytic ozone generating apparatus used in the present invention. [Main component symbol description] 1 Electrolytic ozone generator 2 DC power supply 3 Raw material tank 4 Electrolyzed ozone tank 5 Farmed fish farm 6 Boat 7 Anode 8 Cathode 9 Ion exchange membrane 100113338 23