201144434 六、發明說明： 【發明所屬之技術領域】 本發明係關於培育冬蟲夏草之方法及培養基，尤關於 以人工方式培育冬蟲夏草之方法及培養基。 【先前技術】 夏草疋種學名為•y/wertez’i之真菌，又 名中華**草其可感染昆蟲，使之僵化死亡，進而長出子 貫體，與蟲屍構成冬蟲夏草複合體。 聽、甘露糖醇、維生素m、B2、B12、e及 質、必需脂肪酸及固醇類多醣體化合物等藥 具有藥效而屬於具有商業價值之。 ^ $蟲夏草含有必需氨基酸及蛋白質、蟲草素、蟲莖多 K、稀有礦物 效成分，從而 天然的冬蟲夏草複合體採集不易。201144434 VI. Description of the Invention: [Technical Field of the Invention] The present invention relates to a method and a medium for cultivating Cordyceps sinensis, and more particularly to a method and medium for artificially cultivating Cordyceps sinensis. [Prior Art] The fungus of the summer grass cultivar named y/wertez'i, also known as the Chinese medicinal grass, can infect insects, causing it to die and die, and then grow the child body, and the corpse constitutes the Cordyceps sinensis complex. . Hearing, mannitol, vitamins m, B2, B12, e, and essential, essential fatty acids and sterol-based polysaccharide compounds are commercially effective and have commercial value. ^ Cordyceps contains essential amino acids and proteins, cordycepin, multi-K, and rare mineral-effect components, so that the natural Cordyceps complex is difficult to collect.
冬蟲夏草產物有所需求。 採集不易。從自然環境中取得 集成本極高。藉由養殖昆蟲並 以採收冬蟲夏草複合體之方式 6商業化生產。 方法有待改進，且市場對用 【發明内容】Cordyceps products are in demand. Acquisition is not easy. Getting the integration from the natural environment is extremely high. Commercial production is carried out by breeding insects and collecting the Cordyceps sinensis complex. The method needs to be improved, and the market is used. [Summary of the invention]
法及冬蟲夏草產物。 本發明之目的在於提 冬蟲夏草人工培育方Method and Cordyceps products. The purpose of the present invention is to improve the artificial cultivation of Cordyceps sinensis.
取之技術手段是前述用 201144434 於培養冬蟲夏草的培養基包括有：含量約為_ 之水、令晉介於 0重3：分 …於500至1500重量分之澱粉材料人 於1 〇至35重量分之椰子水、含量約為2重量 3里" 合！介於10至30重量分之大骨湯；以及 白臊 重晉八夕茲她 里）丨於1〇至30 重里刀之庶糖。該用於培養冬益夏草的 蟲夏草人工培育方法。 胃霄如冬The technical means is that the above-mentioned medium for cultivating Cordyceps sinensis with 201144434 includes: water having a content of about _, weight of jin between 0 and 3: minutes... starch of 500 to 1500 parts by weight of the person from 1 35 to 35 parts by weight. Coconut water, the content is about 2 weights 3 miles " Big bone soup between 10 and 30 parts by weight; and white 臊 臊 八 八 她 她 她 她 她 她 她 〇 〇 〇 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 The artificial cultivation method of Cordyceps sinensis for cultivating winter beneficial summer grass. Stomach cramps like winter
f發明亦有關於冬蟲夏草人工培育方法。本發明之冬 ^夏草人卫培育方法包括有：形成—培養基、令前述培養 土降至室溫、將前述培養基置人—容器、加熱前述培養基 至攝氏100度並持續2小時、將前述培養基冷卻至攝氏 1〇·〇-12_0度以取得一經處理的培養基、於前述經處理的培 養基接種冬蟲夏草以形成—培養混合物、令該混合培養物 中的冬蟲夏草生長擴張、令生長擴張之冬蟲夏草成長出子 實體以形成一冬蟲夏草產物。 本發明亦相關於以前述方法形成之冬蟲夏草產物。 藉由本發明可以利用適合商業化生產之培養基及前述 方法大量生產冬蟲夏草，進而提供冬蟲夏草產物滿足市場 需求，達到本發明之目的。 【實施方式】 (一）用於培養冬蟲夏草的培養基 本發明所提供之用於培養冬蟲夏草的培養基，包括 有： 含量約為1 〇〇〇重量分之水； 含量介於500至1500重量分之澱粉材料； 含量介於10至35重量分之椰子水； 4 201144434 含量約為2重量分之蛋白腺； 含量介於10至30重量分之大骨湯；以及 含量介於10至30重量分之蔗糖。 前述大骨湯是將20至60重量分之水加上10至30重 量分之動物骨持續加熱至前述之水蒸散至剩下1 0至30重 量分之液體。 【實施例1】 在本發明之實施例1之培養基包括有： # 含量約為1000重量分之水； 含量約為1 500重量分之澱粉材料； 含量約為2 0重量分之揶子水； 含量約為2重量分之蛋白腺； 含量約為20重量分之大骨湯；以及 含量約為2 0重量分之蔗糖。 該培養基各成分之具體分量可為： 含量約為1公升之水； • 含量約為1.5公斤之澱粉材料； 含量約為20公撮之椰子水； 含量約為2公克之蛋白腺； 含量約為20公撮之大骨湯；以及 含量約為20公克之蔗糖。 在實施例1之中，採用穀類粉末作為前述澱粉材料。 該縠類粉末係將縠類磨粉而成，可以採用的榖類包括有但 不限於稻米、小米、高梁及小麥。 (二）冬蟲夏草人工培育方法 5 .201144434 為形成—種冬蟲夏草產物，本發明所提供之冬蟲夏草 人工培育方法，包括有： 3量约為將1〇〇〇重量分之水含量介於5〇〇15〇〇重 量分之殿粉材料、含量介於1G-35重量分之椰子水、含量 約為2重量分夕疋丄 <蛋白脒、含量介於10_3〇重量分之大骨湯、 含量介於1〇-3〇舍旦八 # 重里刀之庶糖混合煮沸以形成一培養基； 令前述培養基降至室溫； 將前述培袭盆g. 養基置入一谷器，該容器具有容置空間，前 述培養基約佔該容置空間之一半； 加熱前述培養基至攝氏⑽度並持續2小時； 於無菌室將前述培養基冷卻至攝氏1〇 〇12 〇度，藉以 取得一經處理的培養基； β於則逑經處理的培養基接種冬蟲夏草，以形成一培養 混合物； 保持前述培養混合物於攝氏⑽-⑽度並持續一生長 ^，以令邊混合培養物中的冬蟲夏草生長擴張；以及 張之培養混合物於攝氏18.。_2〇 〇度，以令生長擴 夏草成長出子實體’形成-冬蟲夏草產物。 【實施例2】 本發明之實施例2採用前述眘 前述冬蟲夏草人工培育方法。WI之培養基來實施 =之實施例2先煮滞含量約為咖重量分之水、 :、…500重量分之殿粉材料、含量約為2〇重量分之 [S3 分！二t量約為2重量分之蛋白腺、含量約為2〇重量 大月商以及含量約為20重量分之薦糖等成分，以形 6 .201144434 成前述實施例〗之培養基， 蟲夏草產物。 &後續步驟以形成一冬 前述培養基先被降至室溫，再被置入— 是，該容器是玻璃瓶。該容器具有容置谷器。較佳的 至室溫的培養基置入該容 在將刖述降 在實施例2中传.·，'而將该谷器完全填滿。 P 刚述培養基約佔該容置空間之一半。 接者將前述被置入容器 + 之高溫殺菌手段是將脣丞進仃向溫殺菌。常用 基於壓力鋼中加熱至攝氏二;::二容器的培養 ==。在高溫殺菌時，通常將該容二= 方式盍住以保護其容置空 」边礼之 uΜ所衮入的培養基。 ::避免雜菌污染’經過高溫殺菌之前述培養基 於低溫無菌環境中保存備 。土 菌”卷3且“ W用在貫轭例2所採用的低溫無 菌6又備疋具有空調之無菌 ^ . 糟由將則述經過高溫殺菌之 坨養基保存於無菌室，除 苗- ▲ "避免雜菌3染外，亦可藉由無 鹵至的二调達到降溫效果。 在接種冬蟲夏草之前，先今針，+，aβ 1〇 Ο Ί? η ^ ^ 光7則述培養基冷卻至攝氏 1〇.(Μ2·〇度，鈕以取得— ,-由…. 丄處理的培養基。接著，於前述 ，，里處理的培養基接種冬蟲夏草， 4 ®夏早以形成一培養混合物。前 述接種冬蟲夏草之手段是將夂 u, 疋將冬蟲夏草之碎片置於前述培養 基來形成培養混合物。 在丄過接種之後’保持前述培養混合物於攝& 1 〇 〇· 12.0度並持續一生長時間。在實施例2中該生長時間是 三個月，藉以讓該混合培養物中的冬蟲夏草生長擴張。在 理想的狀況下，經渦哇旦士 心生長％•間後，該混合培養物中的冬蟲^ 【S j 7 201144434 夏草可生長擴張至覆蓋該 D培養物整體的程度。 畲經過三個月後 则度，以令生長擴張養混合物於攝氏i8.〇- 冬蟲夏草產物。雖可成— π _ 精由除去殘存之經處理的培養基，s λ如— 括有刖迷成長出子實體之冬蟲夏簟 但在貫施例2之中，Ρ 强夏單’ .^ w ^ 、不另行除去殘存的培養基，因此針 述冬蟲夏草產物包括有前述 因此别 前述經處理的培養基。 焱夏卓以及 此外’可以對於前述夂蟲 '錄夏軍產物進一步進行齡极 磨粉以及製丸之加工處理， 垛、 总认你 慝 以讓則述冬蟲夏草產物形成為 易於使用之劑型，亦7 y*、杜^马 進订乾燥、磨粉將之製成膠囊。 【實施例3】 將前述包括有成長出子實 理的培養基之冬蟲¥直,1 蟲夏草以及前述經處 ' 草產物，進仃乾燥、磨粉後，再加以 化驗可知該冬蟲夏草產物包 叨匕祜有14 ppm之維生素c、 10.63 ppm 之維生辛 e、] π μ ^ i.37 Ppm 之維生素 A、0.15 ppm 之維生素D、11.07 ppm之維生辛 土系ϋ 1、4.0 1 ppm之維生素 B2、m.41柳之維生素B6、23 7 ppm之辞、1171 p㈣ 之鎮、72.2PPm 之鐵、9〇lppm 之每、2 87 ppm 之銅、136 48 ppm之腺苦（adenosine)、之蟲草酸以及2〇78%之水 可溶性多膽體。 此外，前述冬蟲夏草產物具有5.96IU/g之超氧化物歧 化酶之活性。 —從而可去°藉由前述實㈣2㈣成之冬蟲夏草產物確 貫具有冬蟲夏草之主要藥效成分。 201144434 (二）另一冬蟲夏草人工培育方法 為形成則述冬蟲夏草產物，本發明所提供之另一冬蟲 夏草人工培育方法，包括有： 3里勺為將1000重量分之水、含量介於500-1500重 :分之澱：材料、含量介於1〇_35重量分之椰子水含量 人為重里刀之蛋白腺、含量介於1〇_30重量分之大骨湯、 3里介於1〇-30重量分之蔗糖置入一具有容置空間之容器 ^約佔去該容置空間之—部分’進而加熱至攝度並 持續2小時以形成-培養基； ;菌至將如述培養基冷卻至攝氏1〇〇_12〇度； 於别述培養基接種冬蟲夏草，以形成一培養混合物； 、持⑴述培養混合物於攝氏10.0-12.0度並持續三個 得則述培養混合物於攝氏18.0-20.0度，以令生長擴 ' :蟲！草成長出子實體，形成—冬蟲夏草產物。The invention also relates to the artificial cultivation method of Cordyceps sinensis. The winter Xixia grass human health cultivation method of the present invention comprises: forming a medium, lowering the culture soil to room temperature, placing the medium into a human-container, heating the medium to 100 degrees Celsius for 2 hours, and the medium is Cooling to 1 〇·〇-12_0 degrees Celsius to obtain a treated medium, inoculation of Cordyceps sinensis in the above-mentioned treated medium to form a culture mixture, so that the growth of Cordyceps sinensis in the mixed culture is expanded, and the growth of the Cordyceps sinensis grows out. The entity forms a Cordyceps product. The invention is also related to the Cordyceps sinensis product formed by the foregoing method. According to the present invention, it is possible to mass-produce the Cordyceps sinensis using a medium suitable for commercial production and the aforementioned method, thereby providing the Cordyceps sinensis product to meet the market demand, and achieving the object of the present invention. [Embodiment] (1) Medium for cultivating Cordyceps sinensis The medium for cultivating Cordyceps sinensis provided by the present invention comprises: water having a content of about 1 〇〇〇 by weight; starch having a content of 500 to 1500 parts by weight. Material; coconut water having a content of 10 to 35 parts by weight; 4 201144434 protein gland having a content of about 2 parts by weight; a large bone soup having a content of 10 to 30 parts by weight; and sucrose having a content of 10 to 30 parts by weight . The above-mentioned big bone soup is a liquid in which 20 to 60 parts by weight of water plus 10 to 30 parts by weight of the animal bone is continuously heated to the aforementioned water to evaporate to the remaining 10 to 30 parts by weight. [Example 1] The medium of Example 1 of the present invention comprises: #water having a content of about 1000 parts by weight; a starch material having a content of about 1 500 parts by weight; and a water having a content of about 20 parts by weight; A protein gland having a content of about 2 parts by weight; a large bone soup having a content of about 20 parts by weight; and a sucrose having a content of about 20 parts by weight. The specific components of the medium may be: water having a content of about 1 liter; • starch material having a content of about 1.5 kg; coconut water having a content of about 20 metric tons; protein glands having a content of about 2 gram; 20 ounces of big bone soup; and sucrose with a content of about 20 grams. In Example 1, a cereal powder was used as the aforementioned starch material. The anthraquinone powder is obtained by milling a moss, and the types of moss that can be used include, but are not limited to, rice, millet, sorghum, and wheat. (2) Artificial cultivation method of Cordyceps sinensis 5. 201144434 In order to form a kind of Cordyceps sinensis product, the artificial cultivation method of Cordyceps sinensis provided by the present invention comprises: 3 amounts of about 1 〇〇〇 by weight of water content between 5 〇〇 15 〇〇 Weight of the temple powder material, the content of coconut water with a content of 1G-35 parts by weight, the content of about 2 weight points 疋丄 疋丄 脒 脒 脒 含量 含量 含量 含量 含量 含量 含量 含量 含量 含量 含量 含量 、 、 、 、 、 、 、 、 、 1〇-3〇舍旦八# 重 刀 庶 混合 混合 混合 混合 混合 混合 混合 混合 重 重 重 重 重 重 重 重 重 重 重 重 重 重 重 重 重 重 重 重 重 重 重 重 重 重 重 重 重 重 重 重 重 重 重 重 重The medium is about one-half of the accommodation space; the medium is heated to Celsius (10) degrees for 2 hours; the medium is cooled to 1 to 12 degrees Celsius in a sterile room to obtain a treated medium; The treated medium is inoculated with Cordyceps sinensis to form a culture mixture; maintaining the aforementioned culture mixture at (10)-(10) degrees Celsius and continuing a growth to increase the growth of Cordyceps sinensis in the mixed culture Zhang and 18 ° C the mixture was cultured .. 〇 〇 〇 , , , , , , , , , , , , , , , , , , , , , , , [Embodiment 2] In the second embodiment of the present invention, the aforementioned artificial cultivation method of Cordyceps sinensis is used. WI medium to implement = Example 2 first boil the stagnation content of about 500 parts by weight of water, :, ... 500 parts by weight of the temple powder material, the content is about 2 〇 weight points [S3 points! a protein having a protein content of about 2 parts by weight, a product having a content of about 2 parts by weight, and a composition having a content of about 20 parts by weight of a reference sugar, and a medium having the shape of the above-mentioned example, in the form of 6.201144434, a product of the wormwood product . & Subsequent steps to form a winter The aforementioned medium is first lowered to room temperature and then placed in - Yes, the container is a glass bottle. The container has a receptacle. Preferably, the medium to room temperature is placed in the volume and the contents are lowered in Example 2 to completely fill the pot. P Just mentioned that the medium accounts for about half of the accommodation space. The high-temperature sterilization means for placing the aforementioned container into the container is to sterilize the lip liner into the temperature. Commonly used to heat up to two degrees Celsius based on pressure steel;:: culture of two containers ==. In the case of high-temperature sterilization, the volume of the two is usually blocked to protect the medium in which it is placed. :: Avoid contamination of bacteria. The above medium, which has been sterilized by high temperature, is stored in a low temperature sterile environment. Soil bacteria "volume 3 and "W used in the low-temperature sterile 6 used in the yoke example 2 is also prepared to have the air conditioning of the sterile ^. The poorly stored sterilized base is stored in a sterile room, except for seedlings - ▲ "To avoid the contamination of the bacteria 3, it can also achieve the cooling effect by the halogen-free second adjustment. Before inoculation of Cordyceps sinensis, the current needle, +, aβ 1〇Ο Ί η ^ ^ light 7 then the medium is cooled to 1 摄 Celsius (Μ2·〇度, button to obtain -, - by ... ... 丄 treatment of the medium Then, in the above, the medium treated in the inoculation of Cordyceps sinensis, 4 ® early summer to form a culture mixture. The aforementioned method of inoculating the Cordyceps sinensis is to put the pieces of Cordyceps sinensis into the aforementioned medium to form a culture mixture. After the inoculation, 'the aforementioned culture mixture was kept at & 1 〇〇·12.0 degrees and continued for a growth time. In Example 2, the growth time was three months, thereby allowing the growth of Cordyceps sinensis in the mixed culture to expand. Under ideal conditions, after the growth of the vortex, the winter insects in the mixed culture [S j 7 201144434 summer grass can grow and expand to cover the extent of the D culture as a whole. After the month, the degree is increased to grow the mixture in the Celsius i8. 〇- Cordyceps sinensis product. Although it can be made into π _ fine by removing the residual treated medium, s λ, such as In the case of the winter insect worm, but in the second embodiment, Ρ 夏 单 单 . . . 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 冬 冬 冬In addition, 'the above-mentioned locust' recorded Xiajun products can be further processed by age-based milling and pelleting, and 总, always recognize you to make the Cordyceps sinensis product into an easy-to-use dosage form, also 7 y*, Du Ma is ordered to dry and grind to make it into capsules. [Example 3] The above-mentioned winter insects including the medium with the growth of the physique are straight, 1 worm, and the above-mentioned 'grass product, After drying and grinding, it can be confirmed that the Cordyceps product contains 14 ppm of vitamin C, 10.63 ppm of vitamin E, π μ ^ i.37 Ppm of vitamin A, 0.15 ppm of vitamin D, 11.07 ppm of vitamins and earthworms 1, 4.0 1 ppm of vitamin B2, m.41 willow vitamin B6, 23 7 ppm of words, 1171 p (four) of the town, 72.2 ppm of iron, 9 〇lppm each, 2 87 Phenium copper, 136 48 ppm adenosine, Cordycepic acid and 2〇78% of water soluble polycholesterol. In addition, the aforementioned Cordyceps sinensis product has a activity of 5.96 IU/g of superoxide dismutase. - Thus, it can be determined by the above (4) 2 (4) Cordyceps sinensis product. The main medicinal ingredient of Cordyceps sinensis 201144434 (2) Another artificial cultivation method of Cordyceps sinensis is to form the product of Cordyceps sinensis, and another artificial cultivation method of Cordyceps sinensis provided by the present invention includes: 3 tablespoons for 1000 parts by weight of water, The content is between 500-1500 weight: the lake: the material, the content of the coconut water content of 1〇_35 parts by weight, the protein gland of the artificial heavy knife, the content of the bone soup between 1〇_30 parts by weight, 3 miles The sucrose between 1 and 30 parts by weight is placed in a container having an accommodating space, and the portion of the accommodating space is taken up and heated to a temperature for 2 hours to form a medium; The medium is cooled to 1 〇〇 12 ° C; the Cordyceps sinensis is inoculated in a medium to form a culture mixture; and the culture mixture is maintained at 10.0-12.0 degrees Celsius for three times. 18.0-20.0 ° C in the composition, in order to make expansion growth ': worm! The grass grows out of the fruiting body to form the product of Cordyceps sinensis.
女/另冬蟲夏草人工培育方法與前述冬蟲夏草人工培 I谇ί大致相同，其所相異處在於使用該另一冬蟲夏草人 月方法吩，先將各成分依比例裝入容器，The female/other Cordyceps sinensis artificial cultivation method is substantially the same as the above-mentioned Cordyceps sinensis artificial culture I谇ί, and the difference is that the other components of the Cordyceps sinensis are used to firstly load the components into the container in proportion.
兩溫殺菌結合為一個步驟。 U 【實施例4】 明之貫施例4採用前述實施例】之培養基來實施 述另—冬蟲夏草人工培育方法。 含旦：：明之實施例2先將含量約為1〇〇〇重量分之水、 挪二 〇〇重量分之殿粉材料、含量約為20重量分之 子水、含量約為2重量分 之蛋白脒、含1約為20重量[s】 9 201144434 分之大骨湯以及含量約為20重量分之蔗糖等成分置入一 具有容置空間之容器且約佔去該容置空間之一部分，進而 加熱至攝氏1 00度並持續2小時以形成前述實施例1之培 養基，再以該培養基實施前述另一冬蟲夏草人工培育方法 之後續步驟以形成一冬蟲夏草產物。 由上述可知本發明之具體製造及實施方法，其確可解 決冬蟲夏草採集不易和生產困難之問題，並滿足市場之需 求，從而能夠達成本發明之目的。Two-temperature sterilization is combined into one step. U [Example 4] The above Example 4 was carried out by using the medium of the above-mentioned Example to carry out the method of artificially cultivating the Cordyceps sinensis. Inclusion Example 2: Example 2 is a protein having a content of about 1 〇〇〇 by weight of water, a weight of 2 parts by weight of a powder, a content of about 20 parts by weight of water, and a protein content of about 2 parts by weight.脒, containing about 20 weight [s] 9 201144434 points of the big bone soup and the content of about 20 parts by weight of sucrose and the like are placed in a container having a accommodating space and occupying a part of the accommodating space, thereby The mixture was heated to 100 ° C for 2 hours to form the medium of the above Example 1, and the subsequent step of the other method of artificially cultivating the Cordyceps sinensis was carried out with the medium to form a Cordyceps sinensis product. From the above, it is understood that the specific manufacturing and carrying out method of the present invention can solve the problem that the collection of Cordyceps sinensis is difficult and difficult to produce, and meets the needs of the market, thereby achieving the object of the present invention.
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