TW201042038A - Synthetic microcarriers for culturing cells - Google Patents
Synthetic microcarriers for culturing cells Download PDFInfo
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- TW201042038A TW201042038A TW099117055A TW99117055A TW201042038A TW 201042038 A TW201042038 A TW 201042038A TW 099117055 A TW099117055 A TW 099117055A TW 99117055 A TW99117055 A TW 99117055A TW 201042038 A TW201042038 A TW 201042038A
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- microcarrier
- hydrophilic
- unsaturated monomer
- microcarriers
- peptide
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Classifications
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- C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
- C08F289/00—Macromolecular compounds obtained by polymerising monomers on to macromolecular compounds not provided for in groups C08F251/00 - C08F287/00
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
- C08F2/00—Processes of polymerisation
- C08F2/32—Polymerisation in water-in-oil emulsions
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
- C08F220/00—Copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and only one being terminated by only one carboxyl radical or a salt, anhydride ester, amide, imide or nitrile thereof
- C08F220/02—Monocarboxylic acids having less than ten carbon atoms; Derivatives thereof
- C08F220/10—Esters
- C08F220/26—Esters containing oxygen in addition to the carboxy oxygen
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0068—General culture methods using substrates
- C12N5/0075—General culture methods using substrates using microcarriers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0603—Embryonic cells ; Embryoid bodies
- C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
- C08F222/00—Copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and at least one being terminated by a carboxyl radical and containing at least one other carboxyl radical in the molecule; Salts, anhydrides, esters, amides, imides, or nitriles thereof
- C08F222/36—Amides or imides
- C08F222/38—Amides
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2531/00—Microcarriers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/30—Synthetic polymers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/50—Proteins
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
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- Biochemistry (AREA)
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- Oil, Petroleum & Natural Gas (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
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| US18177609P | 2009-05-28 | 2009-05-28 |
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| TW201042038A true TW201042038A (en) | 2010-12-01 |
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| EP (2) | EP2435490A1 (https=) |
| JP (2) | JP5818785B2 (https=) |
| CN (1) | CN102482385B (https=) |
| TW (1) | TW201042038A (https=) |
| WO (2) | WO2010138702A1 (https=) |
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| TWI852996B (zh) * | 2019-02-28 | 2024-08-21 | 日商濱松赫德尼古斯股份有限公司 | 細胞接著用組合物及細胞接著用基材 |
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| JP5560393B2 (ja) * | 2005-06-22 | 2014-07-23 | アステリアス バイオセラピューティクス インコーポレイテッド | 心筋細胞系列細胞への霊長類多能性幹細胞の分化 |
| US8354274B2 (en) * | 2008-01-30 | 2013-01-15 | Geron Corporation | Synthetic surfaces for culturing cells in chemically defined media |
| SG188096A1 (en) | 2008-01-30 | 2013-03-28 | Geron Corp | Synthetic surfaces for culturing stem cell derived oligodendrocyte progenitor cells |
| EP2247718B1 (en) | 2008-01-30 | 2019-01-16 | Asterias Biotherapeutics, Inc. | Synthetic surfaces for culturing stem cell derived cardiomyocytes |
| WO2012048276A2 (en) | 2010-10-08 | 2012-04-12 | Caridianbct, Inc. | Customizable methods and systems of growing and harvesting cells in a hollow fiber bioreactor system |
| US9453197B2 (en) | 2010-12-16 | 2016-09-27 | General Electric Company | Methods of making cell carrier |
| US9534206B2 (en) | 2010-12-16 | 2017-01-03 | General Electric Company | Cell carrier, associated methods for making cell carrier and culturing cells using the same |
| US9926523B2 (en) | 2010-12-16 | 2018-03-27 | General Electric Company | Cell carriers and methods for culturing cells |
| US9453196B2 (en) | 2010-12-16 | 2016-09-27 | General Electric Company | Cell carrier, methods of making and use |
| US9518249B2 (en) | 2010-12-16 | 2016-12-13 | General Electric Company | Cell carrier, associated methods for making cell carrier and culturing cells using the same |
| AU2012256403B2 (en) * | 2011-03-17 | 2016-10-20 | Corning Incorporated | Synthetic coating for cell culture |
| US9376655B2 (en) * | 2011-09-29 | 2016-06-28 | Life Technologies Corporation | Filter systems for separating microcarriers from cell culture solutions |
| KR101886432B1 (ko) * | 2011-12-23 | 2018-09-07 | 엘지디스플레이 주식회사 | 유기전계발광표시장치 및 이의 제조 방법 |
| NZ624195A (en) * | 2012-01-31 | 2015-07-31 | Rogosin Inst | Improved method for manufacture of macrobeads |
| BR112014019157B1 (pt) | 2012-02-02 | 2022-02-22 | Corning Incorporated | Meio de ligação sintético para cultura de célula |
| US10695440B2 (en) * | 2012-05-24 | 2020-06-30 | Biosphere Medical, Inc. | Biomaterials suitable for use as drug eluting, magnetic resonance imaging detectable implants for vascular occlusion |
| EP2666486A1 (en) * | 2012-05-25 | 2013-11-27 | Université de Mons | Micro-engineered hydrogels |
| TWI512103B (zh) * | 2012-11-12 | 2015-12-11 | Ind Tech Res Inst | 細胞培養系統、以及無血清細胞培養方法 |
| JP6299084B2 (ja) * | 2013-05-31 | 2018-03-28 | 大日本印刷株式会社 | 細胞培養方法及びそれに用いるマイクロキャリア |
| EP3013940B1 (en) * | 2013-06-24 | 2017-04-12 | Corning Incorporated | Cell culture article and methods thereof |
| SG11201707615YA (en) | 2013-09-19 | 2017-10-30 | Univ Leland Stanford Junior | Methods and compositions for producing hepatocyte-like cells |
| WO2015073913A1 (en) | 2013-11-16 | 2015-05-21 | Terumo Bct, Inc. | Expanding cells in a bioreactor |
| WO2015148704A1 (en) | 2014-03-25 | 2015-10-01 | Terumo Bct, Inc. | Passive replacement of media |
| CN103980442B (zh) * | 2014-05-29 | 2016-05-18 | 西南石油大学 | 用于稠油乳化降粘的水溶性聚合物及其制备方法 |
| JP6104312B2 (ja) * | 2014-06-19 | 2017-03-29 | 日東電工株式会社 | 組織再生促進剤 |
| EP3198006B1 (en) | 2014-09-26 | 2021-03-24 | Terumo BCT, Inc. | Scheduled feed |
| WO2017004592A1 (en) | 2015-07-02 | 2017-01-05 | Terumo Bct, Inc. | Cell growth with mechanical stimuli |
| US11965175B2 (en) | 2016-05-25 | 2024-04-23 | Terumo Bct, Inc. | Cell expansion |
| US11104874B2 (en) | 2016-06-07 | 2021-08-31 | Terumo Bct, Inc. | Coating a bioreactor |
| US11685883B2 (en) | 2016-06-07 | 2023-06-27 | Terumo Bct, Inc. | Methods and systems for coating a cell growth surface |
| US11873508B2 (en) | 2017-01-20 | 2024-01-16 | Agency For Science, Technology And Research | Thermoresponsive microcarrier system and uses thereof |
| CN117247899A (zh) | 2017-03-31 | 2023-12-19 | 泰尔茂比司特公司 | 细胞扩增 |
| US11624046B2 (en) | 2017-03-31 | 2023-04-11 | Terumo Bct, Inc. | Cell expansion |
| US12234441B2 (en) | 2017-03-31 | 2025-02-25 | Terumo Bct, Inc. | Cell expansion |
| EP3830237A1 (en) * | 2018-08-16 | 2021-06-09 | TERUMO Kabushiki Kaisha | Cell culture substrate |
| WO2020251799A1 (en) | 2019-06-13 | 2020-12-17 | Corning Incorporated | Dissolvable microcarriers for culturing cells and related methods |
| US20240052301A1 (en) * | 2021-02-03 | 2024-02-15 | Sekisui Chemical Co., Ltd. | Microcarrier for cell culture and cell culture method |
| EP4314244B1 (en) | 2021-03-23 | 2025-07-23 | Terumo BCT, Inc. | Cell capture and expansion |
| JP7281116B2 (ja) | 2021-05-11 | 2023-05-25 | 大日本印刷株式会社 | 乾燥マイクロキャリアおよびその製造方法 |
| WO2023049884A2 (en) * | 2021-09-27 | 2023-03-30 | The Regents Of The University Of California | Multivariate biomimetic biomaterial arrays for precision medicine and screening applications |
| US12209689B2 (en) | 2022-02-28 | 2025-01-28 | Terumo Kabushiki Kaisha | Multiple-tube pinch valve assembly |
| USD1099116S1 (en) | 2022-09-01 | 2025-10-21 | Terumo Bct, Inc. | Display screen or portion thereof with a graphical user interface for displaying cell culture process steps and measurements of an associated bioreactor device |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US127878A (en) * | 1872-06-11 | Improvement in plows | ||
| US4070348A (en) | 1973-07-25 | 1978-01-24 | Rohm Gmbh | Water-swellable, bead copolymer |
| SE464816B (sv) | 1985-10-15 | 1991-06-17 | Nilsson Kjell | Makroporoesa partiklar, foerfarande foer dess framstaellning och dess anvaendning |
| WO1989005150A1 (en) | 1987-12-10 | 1989-06-15 | La Jolla Cancer Research Foundation | Conformationally stabilized cell adhesion peptides |
| US5073612A (en) * | 1987-12-28 | 1991-12-17 | Nippon Shokubai Kagaku Kogyo, Co., Ltd. | Hydrophilic polymer and method for production thereof |
| EP0816393B1 (de) * | 1996-07-02 | 2003-11-19 | Vantico AG | Härter für Epoxidharze |
| CN100384958C (zh) * | 1998-12-15 | 2008-04-30 | 艾弗里·丹尼森公司 | 可去除的乳液压敏粘合剂 |
| US20070026518A1 (en) * | 2005-03-29 | 2007-02-01 | The Regents Of The University Of California | Controlling stem cell destiny with tunable matrices |
| WO2004055057A1 (en) | 2002-12-18 | 2004-07-01 | The University Of Melbourne | Hydrogel preparation and process of manufacture thereof |
| US7645827B2 (en) | 2006-03-08 | 2010-01-12 | 3M Innovative Properties Company | High shear pressure-sensitive adhesive |
| MX2009007101A (es) | 2006-12-29 | 2009-12-01 | Univ Washington | Superficies y materiales antibioincrustación bifuncionales. |
| US8354274B2 (en) * | 2008-01-30 | 2013-01-15 | Geron Corporation | Synthetic surfaces for culturing cells in chemically defined media |
| US8329469B2 (en) * | 2008-01-30 | 2012-12-11 | Geron Corporation | Swellable (meth)acrylate surfaces for culturing cells in chemically defined media |
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2010
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- 2010-05-27 US US12/788,917 patent/US9115238B2/en not_active Expired - Fee Related
- 2010-05-27 JP JP2012513247A patent/JP5818785B2/ja not_active Expired - Fee Related
- 2010-05-27 CN CN201080033364.XA patent/CN102482385B/zh not_active Expired - Fee Related
- 2010-05-27 EP EP10721604A patent/EP2435490A1/en not_active Withdrawn
- 2010-05-27 WO PCT/US2010/036380 patent/WO2010138702A1/en not_active Ceased
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- 2010-05-27 EP EP10721249A patent/EP2435489A1/en not_active Withdrawn
- 2010-05-27 WO PCT/US2010/036351 patent/WO2010138687A1/en not_active Ceased
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TWI852996B (zh) * | 2019-02-28 | 2024-08-21 | 日商濱松赫德尼古斯股份有限公司 | 細胞接著用組合物及細胞接著用基材 |
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| US8426176B2 (en) | 2013-04-23 |
| US20100304482A1 (en) | 2010-12-02 |
| JP2012527901A (ja) | 2012-11-12 |
| CN102482385B (zh) | 2014-10-15 |
| JP2012527902A (ja) | 2012-11-12 |
| WO2010138702A1 (en) | 2010-12-02 |
| JP5818785B2 (ja) | 2015-11-18 |
| US20100317113A1 (en) | 2010-12-16 |
| CN102482385A (zh) | 2012-05-30 |
| JP5792714B2 (ja) | 2015-10-14 |
| EP2435490A1 (en) | 2012-04-04 |
| WO2010138687A1 (en) | 2010-12-02 |
| EP2435489A1 (en) | 2012-04-04 |
| US9115238B2 (en) | 2015-08-25 |
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