CN102482385B - 用来培养细胞的合成微型载体 - Google Patents

用来培养细胞的合成微型载体 Download PDF

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Publication number
CN102482385B
CN102482385B CN201080033364.XA CN201080033364A CN102482385B CN 102482385 B CN102482385 B CN 102482385B CN 201080033364 A CN201080033364 A CN 201080033364A CN 102482385 B CN102482385 B CN 102482385B
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China
Prior art keywords
microcarriers
cells
unsaturated monomer
hydrophilic
microcarrier
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Expired - Fee Related
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CN201080033364.XA
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English (en)
Chinese (zh)
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CN102482385A (zh
Inventor
S·德赛
D·亨利
M·赫维
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Corning Inc
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Corning Inc
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Expired - Fee Related legal-status Critical Current
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    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
    • C08F289/00—Macromolecular compounds obtained by polymerising monomers on to macromolecular compounds not provided for in groups C08F251/00 - C08F287/00
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
    • C08F2/00—Processes of polymerisation
    • C08F2/32—Polymerisation in water-in-oil emulsions
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
    • C08F220/00—Copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and only one being terminated by only one carboxyl radical or a salt, anhydride ester, amide, imide or nitrile thereof
    • C08F220/02—Monocarboxylic acids having less than ten carbon atoms; Derivatives thereof
    • C08F220/10—Esters
    • C08F220/26—Esters containing oxygen in addition to the carboxy oxygen
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • C12N5/0075—General culture methods using substrates using microcarriers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
    • C08F222/00—Copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and at least one being terminated by a carboxyl radical and containing at least one other carboxyl radical in the molecule; Salts, anhydrides, esters, amides, imides, or nitriles thereof
    • C08F222/36—Amides or imides
    • C08F222/38—Amides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2531/00—Microcarriers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/30—Synthetic polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Polymers & Plastics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Gynecology & Obstetrics (AREA)
  • Developmental Biology & Embryology (AREA)
  • Microbiology (AREA)
  • Reproductive Health (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN201080033364.XA 2009-05-28 2010-05-27 用来培养细胞的合成微型载体 Expired - Fee Related CN102482385B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US18177609P 2009-05-28 2009-05-28
US61/181,776 2009-05-28
PCT/US2010/036351 WO2010138687A1 (en) 2009-05-28 2010-05-27 Synthetic microcarriers for culturing cells

Publications (2)

Publication Number Publication Date
CN102482385A CN102482385A (zh) 2012-05-30
CN102482385B true CN102482385B (zh) 2014-10-15

Family

ID=42629547

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201080033364.XA Expired - Fee Related CN102482385B (zh) 2009-05-28 2010-05-27 用来培养细胞的合成微型载体

Country Status (6)

Country Link
US (2) US9115238B2 (https=)
EP (2) EP2435490A1 (https=)
JP (2) JP5818785B2 (https=)
CN (1) CN102482385B (https=)
TW (1) TW201042038A (https=)
WO (2) WO2010138702A1 (https=)

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US8354274B2 (en) * 2008-01-30 2013-01-15 Geron Corporation Synthetic surfaces for culturing cells in chemically defined media
SG188096A1 (en) 2008-01-30 2013-03-28 Geron Corp Synthetic surfaces for culturing stem cell derived oligodendrocyte progenitor cells
EP2247718B1 (en) 2008-01-30 2019-01-16 Asterias Biotherapeutics, Inc. Synthetic surfaces for culturing stem cell derived cardiomyocytes
WO2012048276A2 (en) 2010-10-08 2012-04-12 Caridianbct, Inc. Customizable methods and systems of growing and harvesting cells in a hollow fiber bioreactor system
US9453197B2 (en) 2010-12-16 2016-09-27 General Electric Company Methods of making cell carrier
US9534206B2 (en) 2010-12-16 2017-01-03 General Electric Company Cell carrier, associated methods for making cell carrier and culturing cells using the same
US9926523B2 (en) 2010-12-16 2018-03-27 General Electric Company Cell carriers and methods for culturing cells
US9453196B2 (en) 2010-12-16 2016-09-27 General Electric Company Cell carrier, methods of making and use
US9518249B2 (en) 2010-12-16 2016-12-13 General Electric Company Cell carrier, associated methods for making cell carrier and culturing cells using the same
AU2012256403B2 (en) * 2011-03-17 2016-10-20 Corning Incorporated Synthetic coating for cell culture
US9376655B2 (en) * 2011-09-29 2016-06-28 Life Technologies Corporation Filter systems for separating microcarriers from cell culture solutions
KR101886432B1 (ko) * 2011-12-23 2018-09-07 엘지디스플레이 주식회사 유기전계발광표시장치 및 이의 제조 방법
NZ624195A (en) * 2012-01-31 2015-07-31 Rogosin Inst Improved method for manufacture of macrobeads
BR112014019157B1 (pt) 2012-02-02 2022-02-22 Corning Incorporated Meio de ligação sintético para cultura de célula
US10695440B2 (en) * 2012-05-24 2020-06-30 Biosphere Medical, Inc. Biomaterials suitable for use as drug eluting, magnetic resonance imaging detectable implants for vascular occlusion
EP2666486A1 (en) * 2012-05-25 2013-11-27 Université de Mons Micro-engineered hydrogels
TWI512103B (zh) * 2012-11-12 2015-12-11 Ind Tech Res Inst 細胞培養系統、以及無血清細胞培養方法
JP6299084B2 (ja) * 2013-05-31 2018-03-28 大日本印刷株式会社 細胞培養方法及びそれに用いるマイクロキャリア
EP3013940B1 (en) * 2013-06-24 2017-04-12 Corning Incorporated Cell culture article and methods thereof
SG11201707615YA (en) 2013-09-19 2017-10-30 Univ Leland Stanford Junior Methods and compositions for producing hepatocyte-like cells
WO2015073913A1 (en) 2013-11-16 2015-05-21 Terumo Bct, Inc. Expanding cells in a bioreactor
WO2015148704A1 (en) 2014-03-25 2015-10-01 Terumo Bct, Inc. Passive replacement of media
CN103980442B (zh) * 2014-05-29 2016-05-18 西南石油大学 用于稠油乳化降粘的水溶性聚合物及其制备方法
JP6104312B2 (ja) * 2014-06-19 2017-03-29 日東電工株式会社 組織再生促進剤
EP3198006B1 (en) 2014-09-26 2021-03-24 Terumo BCT, Inc. Scheduled feed
WO2017004592A1 (en) 2015-07-02 2017-01-05 Terumo Bct, Inc. Cell growth with mechanical stimuli
US11965175B2 (en) 2016-05-25 2024-04-23 Terumo Bct, Inc. Cell expansion
US11104874B2 (en) 2016-06-07 2021-08-31 Terumo Bct, Inc. Coating a bioreactor
US11685883B2 (en) 2016-06-07 2023-06-27 Terumo Bct, Inc. Methods and systems for coating a cell growth surface
US11873508B2 (en) 2017-01-20 2024-01-16 Agency For Science, Technology And Research Thermoresponsive microcarrier system and uses thereof
CN117247899A (zh) 2017-03-31 2023-12-19 泰尔茂比司特公司 细胞扩增
US11624046B2 (en) 2017-03-31 2023-04-11 Terumo Bct, Inc. Cell expansion
US12234441B2 (en) 2017-03-31 2025-02-25 Terumo Bct, Inc. Cell expansion
EP3830237A1 (en) * 2018-08-16 2021-06-09 TERUMO Kabushiki Kaisha Cell culture substrate
JP7378212B2 (ja) * 2019-02-28 2023-11-13 浜松ホトニクス株式会社 細胞接着用組成物及び細胞接着用基材
WO2020251799A1 (en) 2019-06-13 2020-12-17 Corning Incorporated Dissolvable microcarriers for culturing cells and related methods
US20240052301A1 (en) * 2021-02-03 2024-02-15 Sekisui Chemical Co., Ltd. Microcarrier for cell culture and cell culture method
EP4314244B1 (en) 2021-03-23 2025-07-23 Terumo BCT, Inc. Cell capture and expansion
JP7281116B2 (ja) 2021-05-11 2023-05-25 大日本印刷株式会社 乾燥マイクロキャリアおよびその製造方法
WO2023049884A2 (en) * 2021-09-27 2023-03-30 The Regents Of The University Of California Multivariate biomimetic biomaterial arrays for precision medicine and screening applications
US12209689B2 (en) 2022-02-28 2025-01-28 Terumo Kabushiki Kaisha Multiple-tube pinch valve assembly
USD1099116S1 (en) 2022-09-01 2025-10-21 Terumo Bct, Inc. Display screen or portion thereof with a graphical user interface for displaying cell culture process steps and measurements of an associated bioreactor device

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Also Published As

Publication number Publication date
US8426176B2 (en) 2013-04-23
US20100304482A1 (en) 2010-12-02
JP2012527901A (ja) 2012-11-12
JP2012527902A (ja) 2012-11-12
WO2010138702A1 (en) 2010-12-02
JP5818785B2 (ja) 2015-11-18
US20100317113A1 (en) 2010-12-16
CN102482385A (zh) 2012-05-30
JP5792714B2 (ja) 2015-10-14
EP2435490A1 (en) 2012-04-04
WO2010138687A1 (en) 2010-12-02
EP2435489A1 (en) 2012-04-04
US9115238B2 (en) 2015-08-25
TW201042038A (en) 2010-12-01

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Granted publication date: 20141015

Termination date: 20190527

CF01 Termination of patent right due to non-payment of annual fee