TW200940105A - Inhibitor for growth of bacteria of genus legionella, bath agent and cleaning agent - Google Patents

Inhibitor for growth of bacteria of genus legionella, bath agent and cleaning agent Download PDF

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TW200940105A
TW200940105A TW098106937A TW98106937A TW200940105A TW 200940105 A TW200940105 A TW 200940105A TW 098106937 A TW098106937 A TW 098106937A TW 98106937 A TW98106937 A TW 98106937A TW 200940105 A TW200940105 A TW 200940105A
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Taiwan
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extract
bacteria
inhibitor
growth
legionella
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TW098106937A
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TWI426927B (zh
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Takaki Goto
Syuichi Fukumoto
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Pokka Corp
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08—Magnoliopsida [dicotyledons]
    • A01N65/18—Euphorbiaceae [Spurge family], e.g. ricinus [castorbean]
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
    • A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/335—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin
    • A61K31/35—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having six-membered rings with one oxygen as the only ring hetero atom
    • A61K31/352—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having six-membered rings with one oxygen as the only ring hetero atom condensed with carbocyclic rings, e.g. methantheline 
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/47—Euphorbiaceae (Spurge family), e.g. Ricinus (castorbean)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783—Angiosperms [Magnoliophyta]
    • A61K8/9789—Magnoliopsida [dicotyledons]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/10—Washing or bathing preparations
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
    • A61K2800/52—Stabilizers
    • A61K2800/524—Preservatives
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Description

200940105 六、發明說明： 【發明所屬之技術領域】 本發明係關於退伍軍人桿菌屬菌增殖抑 抑制劑之沐浴劑及洗淨劑。 ⑽ 【先前技術】 退伍軍人桿__喜氧性格蘭氏陰性桿菌 立 易於在例如沐浴設備、冷卻塔中增殖。吸入含有退伍 ^ ❹ ❾ 桿菌感染症。退伍軍人桿菌感^症 矮竹之卒取成分能抑制退伍軍人桿菌屬菌之增殖^ 另方面如專利文獻2戶斤記載，已知 ^:概穌)鋼之萃取物具^ ί r驗軍人桿_ 肢今尚 禾被解Θ在專利文獻2中亦無任何相關記載。 專利文獻1:日本特開2006_45077號公報 專利文獻2 :曰本特開2007-45754號公報 【發明内容】 對於 场⑽菌增殖抑制敝及含树抑_之沐浴劑 軍人ί 目的’在本發明之第一態樣中提供一種退伍 ί萃取物係使駿少含有錢溶狀萃取_從血有桐效萃成取刀而 在本發明之第二態樣中提供一種退伍軍人桿菌屬菌增殖 200940105 抑制劑，其含有選自寧芬酚_A(nymphae〇1_A)、寧 B (2^^hae〇l-B)及寧芬酚-C(nymPhae〇l-C)中之至少一種作為有 斤在^發明之第三態樣中提供一種沐浴劑，其含有前 及第二態樣之退伍軍人桿菌屬菌增殖抑制劑之其中一種。 在本發明之第四態樣中提供一種洗淨劑，其含 及第二態樣之祕軍人㈣韻增殖抑侧之其；第一 【實施方式】 以下，詳細地說明本發明之第一實施態樣。 祕ίΐί ΐ'ί，伍軍人桿菌屬菌增殖抑制劑含有血桐萃 該，萃取物係用至少含有有機溶劑的萃 屬於付。血桐亦被稱作Macarangatanarius，係 而言，其‘長荒繁殖。就血桐 Φ 右的溶劑進行萃取賴的萃取原料’可採用血桐所具 ί十各器官的構成要素。萃取原料’可使用血桐之單獨 構成要素’也可將血桐之二種以上器官或其構成ϋ 殖抑制作用提高，以使用包括血桐之果實、種mi 端部、葉片或分泌物(堰等)之萃取原料為較佳。贫 ί ^ 、根及葉所她b率較高。因此，若使用 為4取原料使用，從易於確保原料之觀點而言，於工業 纽ΐ取補’以採取當時雜‘態，或以採取後經碎裂、破碎 或搗碎的狀態’或以採取及乾燥後經粉碎、破碎或搗碎的狀 200940105 ί取再經乾燥的狀態，提供於 佳。萃取原料的破碎，^原料破碎為較 機(crusher)進行。萃取原料的粉碎，例二機及壓碎 進行。萃取原料的搗碎， 例如可用‘捏 j有機溶劑桐二:::用之_=劑, ❹ ❹ 有機溶劑而言，可單獨使用一插、醇丁醇等。就 使用。若使用水與有S之S劑==容= 機佳為50體積%以上，更佳 可特別有效率=積％以上’ 醇為較佳，以乙醇為更佳3之有效成分。錢溶劑以低碳 化劑在=溶劑中可溶解有例如有機鹽、無機鹽、緩衝劑、乳 中進靡萃取溶劑 Ϊ溫’為了減少不期望:=物劑 仃1取或熱水卒取並除去萃取水之萃取 ΐ具有胁軍人㈣屬_殖_作用之ΐ 孰刀。寧分盼類係屬於水不溶性。藉由將血桐在例如 ί ίΓ。可使寧芬_财卜的祕物核軸鷄至萃取 八取原料萃取出之血桐萃取物，為了將萃取原料的殘潰 i H進打111液分離。111液分離法係藉由，例如，過減、 離心等么知之方法進行。視需要可將赚分離後之紐狀血桐 200940105 萃取物濃縮。 方法，例如，簡由於減壓下加熱而進行，亦可藉峰結$之 而進行 燥 莖敌有有機溶劑之萃取溶劑從血桐萃取出之血桐 卒取物含树自寧芬D名：5,7,3，，4,_四錄_6 = 名.^ 3，4 _四經基_2，_香葉基黃燒鋼(5,7,3，，4，物咖卿_2，_
ΐ 芬紛 _c，（別名：5,7,3，，4，·四經基 ，一甲基烯丙基）-2’_香葉基黃烷酮（5 7 3’，4’-tetrahydroxy-6-(3，，，，3，，，-dimethylallyl)_2，- ，， geran^lflavanone))中之至少一種。金桐萃取物之主要成分係選 自寧芬酚-A(nymphae〇l-A)、寧芬酚_B (nymphae〇1_B)及寧芬酚 -C(nymPhae〇l-C)中之至少一種’亦即為寧芬酚類，其被推測且 有退伍軍人桿菌屬菌增殖抑制作用。 八 在血桐萃取物中更含有蜂膠素A (propolin A)(別名： 5 7^4，:四經基_2，_(7”經基_3，，，7”二甲基_2，，·辛稀基)黃烧嗣 (j,/,3 ,4 -tetrahydroxy.2)-(7--hydroxy-3-,7--dimethyl-2»-〇ctenyl)-flavanon e))。又，在血桐萃取物中包括例如下列微量成分：5,7,3，，4,_ 四羥基-5’-香葉基黃烷酮(別名：異寧芬酚]5)、5,7,3，，4，_四羥基 5 (7 -經基-3，7 -一甲基-2”-辛稀基)黃烧_、5,7,3’,4’-四經基 -6-(7’’-起基_3”，7”-二曱基-2”-辛烯基)黃烷酮、5,入4，_三羥基 -3 -(7舍基-3”，7”-二曱基-2”·辛婦基)黃院酮、5j，4’_三經基_3，_ 香葉基黃烧酮。 在從血桐之各部位萃取出之萃取液之中，從花、種子及果 實(包括蠟)萃取出之萃取液含有特別高濃度之寧芬酚A、b、c 以及異寧芬酚B。 退伍軍人桿菌屬菌增殖抑制劑只要不損及抑制退伍軍人 200940105 軍3 作用可含有血桐萃取物以外之成分。就退伍 :亡ίίί增殖抑制劑所可含有之血桐萃取物以外之成分 賦形劑、基劑、乳化劑、安定劑及香料。 退伍韻增殖抑糊可為餘，亦可為固體狀。 劑、㈣If Γ增殖抑糊之_無特殊_，可為例如散 屈旋劑、膠囊劑、丸劑、液劑。調製成固體 環用菌屬菌增殖抑制劑可由設置於排水用或水循 衣用配除鹵設備中之過濾器負載使用。 〇 ❹ 人士曰高血桐萃取物之水分散性及水溶解性，可於退伍軍 人^屬菌增殖抑制劑中添加，例如，界面活性劑、整合劑 (chelate)、乳化劑或乙醇。 引柄ί I，因吸入含有退伍軍人桿菌屬菌之氣霧(—所 症龐提亞克鮮退伍軍人桿菌感染 益愿t制退伍軍人桿_菌之增殖係極為重要的。退伍軍人桿 增Ϊ抑制劑經由抑制退伍軍人桿_菌之增殖，有益於 預防退伍軍人桿菌屬菌所引起之人類感染。 退伍軍人桿菌屬菌中，雖已知以嗜肺退伍軍人桿菌 feg^idla pneumophila)為代表，但現在已確認出數十種；所 ，此等退伍軍人桿菌屬菌皆被認為有引起退伍軍人桿染 症的可能性。 〜y 退伍軍人桿菌屬菌增殖抑制劑，只要在被推定為退伍軍人 杯囷屬菌增殖之處所，皆可於無特殊限定的適當地使用。具體 而言’退伍軍人桿關g增殖抑侧可添加於，例如，沐^設 備、供水供熱水設備、冷卻塔、加濕器、水景設備、蓄埶槽等 ，使用之水中。由退伍軍人桿菌屬菌引起之感染症對於^抗力 低的尚齡者、新生兒、乳幼兒中特別容易發病。本實施態樣之 退伍軍人桿菌屬菌增殖抑制劑，由於其有效成分係來自天缺物 之血桐萃取物，因此在醫療設施或老人福利設施中可安心地使 用。 退伍軍人桿菌屬菌增殖抑制劑可以，例如，沐浴劑、洗淨 200940105 二ί二前述在可能成為退伍軍人 .=劑以沐浴劑或二】菌増瘦 A、寧芬_及料ϋ人 產切之勒f，寧芬酚 類濃度為】5 ppm以上之方式使用。若寧芬朌 屬菌增殖之作用。剌此良好地發揮抑制退伍軍人桿菌 ❹ ❹ 制劑。沐浴劑之劑ΐ可：二二人菌增殖抑 例如水雜域it it f 含有選自 維生素類、生_、:^調整劑、 石夕酸納等石夕^ 納或碳酸氫鋼等碳酸鹽；偏 鹽，5化納、氯简、氯化銨等氯化物。鈉次硫_等硫酸 浴钟ίΐΐί等浴劑混合於沐浴設備之浴液中使用，可以於沐 其時之洛槽、該浴槽中循環浴液之配管、接續於談配 二本&器^熱父士換器中抑制退伍軍人桿菌屬菌之增、殖=因 浴=劑52 fΙίΐ中ί桐萃取物之含量，可視混入 中寧答8^Α當且地设疋。就沐浴劑而言，期望以使浴液 B '寧芬朌C之合計濃度，脚寧芬ί „度雖為l5ppm以上’更佳為2〇醇以上 ^在浴液巾寧芬賴濃度為15 ppm社，制能良^ 抑制退伍軍人桿菌屬菌增殖之作用。 綱本樣之洗淨劑含有前述退伍軍人桿菌屬菌增殖抑 :，Ϊ為固體狀、液狀、粉末狀。在洗淨 ίΐ見Ϊ了别輕軍人桿賴菌增殖抑制劑之外，含有選自 例如界面雜劑、螯合劑、ΡΗ調整财之至少—種。洗淨 1， 200940105 視需要可進一步含有氯系或氧系除菌劑。 軍人用在與可能成為退伍 稀釋洗淨劑並浸潰可能成為退伍軍人^菌可屬用ff液適當地 :生可 用態樣而二=:=之二量成劑之使 ❹ 特別能良舰發揮抑舰伍15鹏以上’ 根據本實施態樣，得到下列:士果。函曰殖之作用。 取物 ==桿_之増=於==^^ ^事===:= 料之供 益Μ 2施祕之沐浴航洗淨㈣於含㈣述退伍軍人桿 =增殖抑制劑，所以具有抑制退伍軍人桿菌屬菌= 前述各實施態樣可變更如下。 ，述實施態樣之退伍軍人桿菌屬菌增_制劑 來，血桐萃取物以外之寧麵A、寧細8及寧芬紛C中 之至少一種以代替血桐萃取物作為有效， 等成分。來自血桐萃取物以外之寧芬酚A、^J酚 B及+祕C ’例如’可藉由化學合成而制。 τ㈣ 退伍軍人桿菌屬菌存在於土壤等自然環境中。因此 軍人桿菌屬菌在樹脂製袋子密封保存之園藝用土或肥料中恐 200940105 =會增殖。因此，為了防止人類經 軍人桿菌屬菌感染，可將前述實施離樣:，或肥料被退伍 用添加退伍軍人桿朗菌增殖抑制物，所以使 物之繁殖亦幾乎無^何^礙來 _進—步頻本發明。 〈血桐萃取物之調製1>
碎。取並經冷;東的蝴葉片解壤，並用剪刀煎 ⑽室溫下以靜置的狀態浸潰於90體積 t Ϊ體積份之水所構成之混合溶劑100 ml中兩個星 月。之後，將其過濾並回收濾液以作為血桐萃取液。 乾該血桐萃取細將該血桐萃取液所含之__製成^ 士，之血桐萃取物。該粉末狀之血桐萃取物所含之寧芬酚A、 f芬酚B及寧芬酚C之合計濃度，亦即寧芬酚類濃度，從下 述HPLC條件下分析血桐萃取物所得之示於第一圖之層析圖 計算，結果為50質量％。 HPLC條件： 系統 ：PDA-HPLC系統(島津製作所）、LClOADvp系 列、UV ; SPD-lOAvp、PDA ; SPD-M10Avp 管柱 ：Luna C18 (2.0 X 250 mm)(島津 GLC) 溶劑 ·· A :水(5%醋酸）、B :乙腈(5%醋酸） 溶出條件：0-20分鐘 (梯度溶析；A : B=80 : 20 —A : B = 30 : 70) 20-50分鐘 (梯度溶析；A : B=30 : 70 ->A : B = 0 : 100) 50-60 分鐘(A : B = 0 : 1〇〇) 60-75 分鐘(A : B = 80 : 20) 200940105 流速 ：0.2毫升/分鐘
PDA 檢測：UVi90-370nm UV 檢測：UV287nm 注入量 ：20 μΐ 溫度 ：40〇C <血桐萃取物對於退伍軍人桿菌屬菌之抗菌活性試驗> 前培養 於37°C ’使用添加有L-半胱胺酸之緩衝活性碳酵母萃取 ❹ 物^BCYE)培養基培養退伍軍人桿菌屬菌株，即嗜肺退伍軍人 桿菌(Legionella pneumophila)(GIFU9134)計四日後，藉由將所 得到之菌體懸浮於磷酸緩衝液中，調製菌數為l〇6〜1〇7CFU/ml 之接種用菌液。 檢體液之調製 將前述粉末狀之血桐萃取物混合於99.5V/V%乙醇中並調 製血桐萃取物之濃度為1〇〇 x 1〇3 ppm之檢體液。再者，藉由 將該檢體液用99.5 V/V%乙醇稀釋，調製2倍稀釋系列之檢體 液’亦即血桐萃取物之濃度分別為50 X 1〇3 ppm、25 X 1〇3 ppm、12.5x 103ppm、6.25x 103ppm、3.13x 1〇3 ppm、1.56 X 103 〇 ppm之檢體液。 感受性測定用平板培養基之調製 在99質量％之滅菌後保溫於50〜60°C之B-SYE-α中添加1 質量％之各檢體液，充分混合後，分注於培養皿(Schale)，使 之固化。然後，調製血桐萃取物之濃度分別為l，000ppm、5〇〇 ppm、250 ppm、125 ppm、62.5 ppm、31.3 ppm、15.6 ppm 之 感受性測定用平板培養基。將調製後之平板培養基在潔淨工作 檯内乾燥。再者，B-SYE-α培養基具有澱粉1.5 g、酵母萃取 物1.0 g、瓊脂1.3 g、退伍軍人桿菌BCYEa生長補充劑 (Legionella BCYE a Growth Supplement, OXOID) 1 /kiisTviaH 及精製水100 ml之組成。 11 200940105 退伍軍人桿菌屬菌之接種及培養 使用内徑1 mm之環(l〇op)將接種用菌液劃線塗 性測定用平板培養基。之後，將各平板培養基上之菌株於一 培養三天。 ' 判定及結果 觀察培養後之平板培養基時，確認在血桐萃取物 31.3 ppm以上之平板培養基上可阻止退伍軍人桿菌 ======= ❹ ❹ 實施例2 <血·桐萃取物之調製2> 桐葉片3Gg浸潰於95°C之水30分鐘。將 Ϊ由，除去水之殘餘葉片浸潰於100%乙醇：天，之後，將 液以血桐萃取液回收。藉由將=以 該粉末狀之血桐萃取物所含之*狀之血桐卒取物。 之合計濃度，亦 :桐萃取物所得之示於第二圖之層析圖計t ，結果^ 40質量 屬产抗菌活性試驗> A桐萃取物得職實施例雜，實補2所調製之 之血桐萃取物之抗菌活性4樣桐卒取物與實施例2所調製 【圖式簡單說明】 之灰桐萃 取物之結果的層析圖譜。 速軸層析分析實施例！ 200940105 第二圖係顯示使用高速液相層析分析實施例2之血桐萃 取物之結果的層析圖譜。 【主要元件符號說明】
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Claims (1)

  1. 200940105 七、申請專利範圍： L 一種退伍軍人桿菌屬菌增殖抑制劑，其特徵為含有血桐萃 2. ^物作為有效成分，該萃取物係使用至少含有有機溶劑之 萃取溶劑從血桐萃取而得。 —種退伍軍人桿菌屬菌增殖抑制劑，其特徵為含有選自寧 劣盼-A(nymphaeol-A)、寧芬酚_b (nymphae〇i_B)及寧芬紛 3. -C(nymphae〇l-C)中之至少一種作為有效成分。 Ο 4. HI，退伍軍人桿菌屬菌增殖抑制劑， 係來自乂血桐寧务㈣及寧芬_中之至少一種 如申請專利範圍第1至3項中任-項 之退伍軍人柃_屬函増殖抑制劑。 如申請專利範圍第1至3項中任-項 退伍軍人杯囷屬菌增殖抑制劑。 5.
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US11395931B2 (en) 2017-12-02 2022-07-26 Mighty Fire Breaker Llc Method of and system network for managing the application of fire and smoke inhibiting compositions on ground surfaces before the incidence of wild-fires, and also thereafter, upon smoldering ambers and ashes to reduce smoke and suppress fire re-ignition
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US11865394B2 (en) 2017-12-03 2024-01-09 Mighty Fire Breaker Llc Environmentally-clean biodegradable water-based concentrates for producing fire inhibiting and fire extinguishing liquids for fighting class A and class B fires
US11865390B2 (en) 2017-12-03 2024-01-09 Mighty Fire Breaker Llc Environmentally-clean water-based fire inhibiting biochemical compositions, and methods of and apparatus for applying the same to protect property against wildfire
US11826592B2 (en) 2018-01-09 2023-11-28 Mighty Fire Breaker Llc Process of forming strategic chemical-type wildfire breaks on ground surfaces to proactively prevent fire ignition and flame spread, and reduce the production of smoke in the presence of a wild fire
CN108815237A (zh) * 2018-08-27 2018-11-16 维康腾达生物科技有限公司 一种博落回提取物的制备方法
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CN101959408A (zh) 2011-01-26
EP2255624A1 (en) 2010-12-01
EP2255624A4 (en) 2013-05-22
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JP5563199B2 (ja) 2014-07-30

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