TW200813232A

TW200813232A - Rationale, methods, and assays for identifying novel taste cell genes and salty taste receptor targets and assays using these identified genes or gene products

Rationale, methods, and assays for identifying novel taste cell genes and salty taste receptor targets and assays using these identified genes or gene products Download PDF

Info

Publication number: TW200813232A
Authority: TW; Taiwan
Prior art keywords: taste; cell; cells; gene; genes
Prior art date: 2006-06-08

Application number

TW096120717A

Other languages

English (en)

Chinese (zh)

Inventor

Bryan Moyer

Min Lu

Fernando Echeverri

Dalia Kalabat

Na Gao

Peter Hevezi

Original Assignee

Senomyx Inc

Priority date

2006-06-08

Filing date

2007-06-08

Publication date

2008-03-16

2007-06-08 Application filed by Senomyx Inc filed Critical Senomyx Inc

2008-03-16 Publication of TW200813232A publication Critical patent/TW200813232A/zh

Links

235000019640 taste Nutrition 0.000 title claims abstract description 688
108090000623 proteins and genes Proteins 0.000 title claims abstract description 612
238000000034 method Methods 0.000 title claims abstract description 225
238000003556 assay Methods 0.000 title claims abstract description 62
108091005708 gustatory receptors Proteins 0.000 title claims abstract description 43
235000019643 salty taste Nutrition 0.000 title claims description 38
210000004027 cell Anatomy 0.000 claims abstract description 711
150000001875 compounds Chemical class 0.000 claims abstract description 197
230000000694 effects Effects 0.000 claims abstract description 96
235000019583 umami taste Nutrition 0.000 claims abstract description 67
230000006870 function Effects 0.000 claims abstract description 61
230000033228 biological regulation Effects 0.000 claims abstract description 42
235000009508 confectionery Nutrition 0.000 claims abstract description 41
235000019658 bitter taste Nutrition 0.000 claims abstract description 38
108020003175 receptors Proteins 0.000 claims abstract description 28
239000003814 drug Substances 0.000 claims abstract description 27
230000001079 digestive effect Effects 0.000 claims abstract description 26
208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims abstract description 25
235000019607 umami taste sensations Nutrition 0.000 claims abstract description 20
210000001035 gastrointestinal tract Anatomy 0.000 claims abstract description 19
235000019614 sour taste Nutrition 0.000 claims abstract description 19
201000010099 disease Diseases 0.000 claims abstract description 18
241000124008 Mammalia Species 0.000 claims abstract description 14
208000008589 Obesity Diseases 0.000 claims abstract description 14
239000011324 bead Substances 0.000 claims abstract description 14
235000020824 obesity Nutrition 0.000 claims abstract description 14
238000001943 fluorescence-activated cell sorting Methods 0.000 claims abstract description 13
235000019605 sweet taste sensations Nutrition 0.000 claims abstract description 13
150000003839 salts Chemical class 0.000 claims abstract description 4
231100000599 cytotoxic agent Toxicity 0.000 claims abstract description 3
239000002619 cytotoxin Substances 0.000 claims abstract description 3
230000002503 metabolic effect Effects 0.000 claims abstract description 3
102000004169 proteins and genes Human genes 0.000 claims description 137
230000014509 gene expression Effects 0.000 claims description 88
108090000862 Ion Channels Proteins 0.000 claims description 79
102000004310 Ion Channels Human genes 0.000 claims description 78
210000002105 tongue Anatomy 0.000 claims description 72
238000004458 analytical method Methods 0.000 claims description 66
108090000765 processed proteins & peptides Proteins 0.000 claims description 57
101000605625 Homo sapiens Polycystic kidney disease 2-like 1 protein Proteins 0.000 claims description 56
102100038330 Polycystic kidney disease 2-like 1 protein Human genes 0.000 claims description 55
101000844521 Homo sapiens Transient receptor potential cation channel subfamily M member 5 Proteins 0.000 claims description 52
102000003609 TRPM5 Human genes 0.000 claims description 50
239000011734 sodium Substances 0.000 claims description 49
DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims description 48
229910052708 sodium Inorganic materials 0.000 claims description 48
210000001519 tissue Anatomy 0.000 claims description 43
239000012528 membrane Substances 0.000 claims description 41
210000001779 taste bud Anatomy 0.000 claims description 36
238000012360 testing method Methods 0.000 claims description 33
108091032973 (ribonucleotides)n+m Proteins 0.000 claims description 31
108091006146 Channels Proteins 0.000 claims description 30
150000002500 ions Chemical class 0.000 claims description 30
238000012216 screening Methods 0.000 claims description 29
239000000523 sample Substances 0.000 claims description 28
230000029087 digestion Effects 0.000 claims description 27
230000001747 exhibiting effect Effects 0.000 claims description 27
210000000214 mouth Anatomy 0.000 claims description 27
102000004196 processed proteins & peptides Human genes 0.000 claims description 27
102000005962 receptors Human genes 0.000 claims description 27
210000000130 stem cell Anatomy 0.000 claims description 27
239000000126 substance Substances 0.000 claims description 26
206010028980 Neoplasm Diseases 0.000 claims description 24
201000011510 cancer Diseases 0.000 claims description 24
229940088597 hormone Drugs 0.000 claims description 24
239000005556 hormone Substances 0.000 claims description 24
208000024891 symptom Diseases 0.000 claims description 24
101000730587 Homo sapiens Polycystic kidney disease protein 1-like 3 Proteins 0.000 claims description 23
238000007423 screening assay Methods 0.000 claims description 23
238000011282 treatment Methods 0.000 claims description 23
102100032598 Polycystic kidney disease protein 1-like 3 Human genes 0.000 claims description 22
235000013305 food Nutrition 0.000 claims description 22
230000002496 gastric effect Effects 0.000 claims description 22
102000004190 Enzymes Human genes 0.000 claims description 20
108090000790 Enzymes Proteins 0.000 claims description 20
210000002249 digestive system Anatomy 0.000 claims description 20
239000000975 dye Substances 0.000 claims description 20
239000003550 marker Substances 0.000 claims description 20
238000001514 detection method Methods 0.000 claims description 18
210000003802 sputum Anatomy 0.000 claims description 18
206010036790 Productive cough Diseases 0.000 claims description 17
238000002955 isolation Methods 0.000 claims description 17
208000024794 sputum Diseases 0.000 claims description 17
230000001225 therapeutic effect Effects 0.000 claims description 17
108010078791 Carrier Proteins Proteins 0.000 claims description 16
229920001184 polypeptide Polymers 0.000 claims description 16
238000000746 purification Methods 0.000 claims description 16
-1 triethylammonium salt Chemical class 0.000 claims description 16
230000027455 binding Effects 0.000 claims description 15
230000001105 regulatory effect Effects 0.000 claims description 15
210000004962 mammalian cell Anatomy 0.000 claims description 14
210000002784 stomach Anatomy 0.000 claims description 14
239000002253 acid Substances 0.000 claims description 13
229940079593 drug Drugs 0.000 claims description 13
238000002372 labelling Methods 0.000 claims description 13
210000000287 oocyte Anatomy 0.000 claims description 13
208000011580 syndromic disease Diseases 0.000 claims description 13
230000032258 transport Effects 0.000 claims description 13
241001465754 Metazoa Species 0.000 claims description 12
230000009102 absorption Effects 0.000 claims description 12
238000010521 absorption reaction Methods 0.000 claims description 12
210000000987 immune system Anatomy 0.000 claims description 12
230000002757 inflammatory effect Effects 0.000 claims description 12
230000000984 immunochemical effect Effects 0.000 claims description 11
230000004060 metabolic process Effects 0.000 claims description 11
230000003321 amplification Effects 0.000 claims description 10
230000001363 autoimmune Effects 0.000 claims description 10
238000007901 in situ hybridization Methods 0.000 claims description 10
238000003199 nucleic acid amplification method Methods 0.000 claims description 10
210000000056 organ Anatomy 0.000 claims description 10
235000019608 salt taste sensations Nutrition 0.000 claims description 10
208000011231 Crohn disease Diseases 0.000 claims description 9
206010009900 Colitis ulcerative Diseases 0.000 claims description 8
201000006704 Ulcerative Colitis Diseases 0.000 claims description 8
230000005540 biological transmission Effects 0.000 claims description 8
201000006549 dyspepsia Diseases 0.000 claims description 8
238000001727 in vivo Methods 0.000 claims description 8
210000002569 neuron Anatomy 0.000 claims description 8
241000894007 species Species 0.000 claims description 8
210000000108 taste bud cell Anatomy 0.000 claims description 8
102400000921 Gastrin Human genes 0.000 claims description 7
108010052343 Gastrins Proteins 0.000 claims description 7
AOXOCDRNSPFDPE-UKEONUMOSA-N chembl413654 Chemical compound C([C@H](C(=O)NCC(=O)N[C@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@H](CCSC)C(=O)N[C@H](CC(O)=O)C(=O)N[C@H](CC=1C=CC=CC=1)C(N)=O)NC(=O)[C@@H](C)NC(=O)[C@@H](CCC(O)=O)NC(=O)[C@@H](CCC(O)=O)NC(=O)[C@@H](CCC(O)=O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC=1C2=CC=CC=C2NC=1)NC(=O)[C@H]1N(CCC1)C(=O)CNC(=O)[C@@H](N)CCC(O)=O)C1=CC=C(O)C=C1 AOXOCDRNSPFDPE-UKEONUMOSA-N 0.000 claims description 7
238000002512 chemotherapy Methods 0.000 claims description 7
230000006378 damage Effects 0.000 claims description 7
230000001419 dependent effect Effects 0.000 claims description 7
235000003642 hunger Nutrition 0.000 claims description 7
210000002865 immune cell Anatomy 0.000 claims description 7
230000036039 immunity Effects 0.000 claims description 7
239000003068 molecular probe Substances 0.000 claims description 7
210000001635 urinary tract Anatomy 0.000 claims description 7
230000000692 anti-sense effect Effects 0.000 claims description 6
230000036528 appetite Effects 0.000 claims description 6
235000019789 appetite Nutrition 0.000 claims description 6
230000004069 differentiation Effects 0.000 claims description 6
210000003238 esophagus Anatomy 0.000 claims description 6
230000029142 excretion Effects 0.000 claims description 6
230000001575 pathological effect Effects 0.000 claims description 6
230000035479 physiological effects, processes and functions Effects 0.000 claims description 6
238000012106 screening analysis Methods 0.000 claims description 6
230000028327 secretion Effects 0.000 claims description 6
229910001415 sodium ion Inorganic materials 0.000 claims description 6
108090000045 G-Protein-Coupled Receptors Proteins 0.000 claims description 5
102000003688 G-Protein-Coupled Receptors Human genes 0.000 claims description 5
PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 5
238000001479 atomic absorption spectroscopy Methods 0.000 claims description 5
230000003305 autocrine Effects 0.000 claims description 5
210000001072 colon Anatomy 0.000 claims description 5
208000010643 digestive system disease Diseases 0.000 claims description 5
235000011389 fruit/vegetable juice Nutrition 0.000 claims description 5
238000003384 imaging method Methods 0.000 claims description 5
230000000968 intestinal effect Effects 0.000 claims description 5
230000037427 ion transport Effects 0.000 claims description 5
210000005036 nerve Anatomy 0.000 claims description 5
230000003076 paracrine Effects 0.000 claims description 5
230000002265 prevention Effects 0.000 claims description 5
238000010361 transduction Methods 0.000 claims description 5
230000026683 transduction Effects 0.000 claims description 5
238000012546 transfer Methods 0.000 claims description 5
206010006326 Breath odour Diseases 0.000 claims description 4
102000051325 Glucagon Human genes 0.000 claims description 4
108060003199 Glucagon Proteins 0.000 claims description 4
WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 4
241000283984 Rodentia Species 0.000 claims description 4
108020004459 Small interfering RNA Proteins 0.000 claims description 4
208000027418 Wounds and injury Diseases 0.000 claims description 4
241000269370 Xenopus <genus> Species 0.000 claims description 4
210000000436 anus Anatomy 0.000 claims description 4
230000011712 cell development Effects 0.000 claims description 4
206010013781 dry mouth Diseases 0.000 claims description 4
230000004064 dysfunction Effects 0.000 claims description 4
210000000232 gallbladder Anatomy 0.000 claims description 4
MASNOZXLGMXCHN-ZLPAWPGGSA-N glucagon Chemical compound C([C@@H](C(=O)N[C@H](C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(O)=O)C(C)C)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](C)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CO)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CO)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CO)NC(=O)[C@@H](NC(=O)[C@H](CC=1C=CC=CC=1)NC(=O)[C@@H](NC(=O)CNC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CO)NC(=O)[C@@H](N)CC=1NC=NC=1)[C@@H](C)O)[C@@H](C)O)C1=CC=CC=C1 MASNOZXLGMXCHN-ZLPAWPGGSA-N 0.000 claims description 4
229960004666 glucagon Drugs 0.000 claims description 4
239000008103 glucose Substances 0.000 claims description 4
208000014674 injury Diseases 0.000 claims description 4
QQVIHTHCMHWDBS-UHFFFAOYSA-N isophthalic acid Chemical compound OC(=O)C1=CC=CC(C(O)=O)=C1 QQVIHTHCMHWDBS-UHFFFAOYSA-N 0.000 claims description 4
210000004185 liver Anatomy 0.000 claims description 4
238000010992 reflux Methods 0.000 claims description 4
241000251468 Actinopterygii Species 0.000 claims description 3
102000013142 Amylases Human genes 0.000 claims description 3
108010065511 Amylases Proteins 0.000 claims description 3
208000007882 Gastritis Diseases 0.000 claims description 3
241000239226 Scorpiones Species 0.000 claims description 3
208000025371 Taste disease Diseases 0.000 claims description 3
208000025865 Ulcer Diseases 0.000 claims description 3
238000007792 addition Methods 0.000 claims description 3
235000019418 amylase Nutrition 0.000 claims description 3
239000006285 cell suspension Substances 0.000 claims description 3
208000007784 diverticulitis Diseases 0.000 claims description 3
208000024798 heartburn Diseases 0.000 claims description 3
210000002429 large intestine Anatomy 0.000 claims description 3
239000000463 material Substances 0.000 claims description 3
235000019656 metallic taste Nutrition 0.000 claims description 3
VYNDHICBIRRPFP-UHFFFAOYSA-N pacific blue Chemical compound FC1=C(O)C(F)=C2OC(=O)C(C(=O)O)=CC2=C1 VYNDHICBIRRPFP-UHFFFAOYSA-N 0.000 claims description 3
210000002243 primary neuron Anatomy 0.000 claims description 3
210000000813 small intestine Anatomy 0.000 claims description 3
238000001356 surgical procedure Methods 0.000 claims description 3
231100000397 ulcer Toxicity 0.000 claims description 3
239000004382 Amylase Substances 0.000 claims description 2
241000282693 Cercopithecidae Species 0.000 claims description 2
101800001982 Cholecystokinin Proteins 0.000 claims description 2
102100025841 Cholecystokinin Human genes 0.000 claims description 2
101710112752 Cytotoxin Proteins 0.000 claims description 2
108010088406 Glucagon-Like Peptides Proteins 0.000 claims description 2
206010061218 Inflammation Diseases 0.000 claims description 2
102100033420 Keratin, type I cytoskeletal 19 Human genes 0.000 claims description 2
108010066302 Keratin-19 Proteins 0.000 claims description 2
239000004472 Lysine Substances 0.000 claims description 2
KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 claims description 2
102000045595 Phosphoprotein Phosphatases Human genes 0.000 claims description 2
108700019535 Phosphoprotein Phosphatases Proteins 0.000 claims description 2
230000008901 benefit Effects 0.000 claims description 2
MWUYLQGOGOLRQS-UHFFFAOYSA-N bis(acetyloxymethyl) 4-[6-[13-[2-[2,4-bis(acetyloxymethoxycarbonyl)phenyl]-5-methoxy-1-benzofuran-6-yl]-1,4,10-trioxa-7,13-diazacyclopentadec-7-yl]-5-methoxy-1-benzofuran-2-yl]benzene-1,3-dicarboxylate Chemical compound COC1=CC=2C=C(C=3C(=CC(=CC=3)C(=O)OCOC(C)=O)C(=O)OCOC(C)=O)OC=2C=C1N(CCOCC1)CCOCCOCCN1C(C(=CC=1C=2)OC)=CC=1OC=2C1=CC=C(C(=O)OCOC(C)=O)C=C1C(=O)OCOC(C)=O MWUYLQGOGOLRQS-UHFFFAOYSA-N 0.000 claims description 2
229940107137 cholecystokinin Drugs 0.000 claims description 2
208000001848 dysentery Diseases 0.000 claims description 2
235000019581 fat taste sensations Nutrition 0.000 claims description 2
230000006698 induction Effects 0.000 claims description 2
230000004054 inflammatory process Effects 0.000 claims description 2
125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 claims description 2
239000003607 modifier Substances 0.000 claims description 2
IZTQOLKUZKXIRV-YRVFCXMDSA-N sincalide Chemical compound C([C@@H](C(=O)N[C@@H](CCSC)C(=O)NCC(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC=1C=CC=CC=1)C(N)=O)NC(=O)[C@@H](N)CC(O)=O)C1=CC=C(OS(O)(=O)=O)C=C1 IZTQOLKUZKXIRV-YRVFCXMDSA-N 0.000 claims description 2
238000010230 functional analysis Methods 0.000 claims 3
238000002825 functional assay Methods 0.000 claims 3
230000007661 gastrointestinal function Effects 0.000 claims 3
AZQWKYJCGOJGHM-UHFFFAOYSA-N 1,4-benzoquinone Chemical compound O=C1C=CC(=O)C=C1 AZQWKYJCGOJGHM-UHFFFAOYSA-N 0.000 claims 2
HAPJROQJVSPKCJ-UHFFFAOYSA-N 3-[4-[2-[6-(dibutylamino)naphthalen-2-yl]ethenyl]pyridin-1-ium-1-yl]propane-1-sulfonate Chemical compound C1=CC2=CC(N(CCCC)CCCC)=CC=C2C=C1C=CC1=CC=[N+](CCCS([O-])(=O)=O)C=C1 HAPJROQJVSPKCJ-UHFFFAOYSA-N 0.000 claims 2
208000018522 Gastrointestinal disease Diseases 0.000 claims 2
DTHNMHAUYICORS-KTKZVXAJSA-N Glucagon-like peptide 1 Chemical compound C([C@@H](C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](C)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CCCCN)C(=O)NCC(=O)N[C@@H](CCCNC(N)=N)C(N)=O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CCCCN)NC(=O)[C@H](C)NC(=O)[C@H](C)NC(=O)[C@H](CCC(N)=O)NC(=O)CNC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CO)NC(=O)[C@H](CO)NC(=O)[C@@H](NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CO)NC(=O)[C@@H](NC(=O)[C@H](CC=1C=CC=CC=1)NC(=O)[C@@H](NC(=O)CNC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](C)NC(=O)[C@@H](N)CC=1N=CNC=1)[C@@H](C)O)[C@@H](C)O)C(C)C)C1=CC=CC=C1 DTHNMHAUYICORS-KTKZVXAJSA-N 0.000 claims 2
102100040918 Pro-glucagon Human genes 0.000 claims 2
MJOQJPYNENPSSS-XQHKEYJVSA-N [(3r,4s,5r,6s)-4,5,6-triacetyloxyoxan-3-yl] acetate Chemical compound CC(=O)O[C@@H]1CO[C@@H](OC(C)=O)[C@H](OC(C)=O)[C@H]1OC(C)=O MJOQJPYNENPSSS-XQHKEYJVSA-N 0.000 claims 2
210000004798 organs belonging to the digestive system Anatomy 0.000 claims 2
235000019600 saltiness Nutrition 0.000 claims 2
229920002554 vinyl polymer Polymers 0.000 claims 2
230000002747 voluntary effect Effects 0.000 claims 2
DLFVBJFMPXGRIB-UHFFFAOYSA-N Acetamide Chemical compound CC(N)=O DLFVBJFMPXGRIB-UHFFFAOYSA-N 0.000 claims 1
206010006707 Burn oesophageal Diseases 0.000 claims 1
241000867607 Chlorocebus sabaeus Species 0.000 claims 1
108090000695 Cytokines Proteins 0.000 claims 1
101710198884 GATA-type zinc finger protein 1 Proteins 0.000 claims 1
206010017943 Gastrointestinal conditions Diseases 0.000 claims 1
102000014150 Interferons Human genes 0.000 claims 1
108010050904 Interferons Proteins 0.000 claims 1
OFOBLEOULBTSOW-UHFFFAOYSA-N Malonic acid Chemical compound OC(=O)CC(O)=O OFOBLEOULBTSOW-UHFFFAOYSA-N 0.000 claims 1
JQVDAXLFBXTEQA-UHFFFAOYSA-N N-butyl-butylamine Natural products CCCCNCCCC JQVDAXLFBXTEQA-UHFFFAOYSA-N 0.000 claims 1
SUHOOTKUPISOBE-UHFFFAOYSA-N O-phosphoethanolamine Chemical compound NCCOP(O)(O)=O SUHOOTKUPISOBE-UHFFFAOYSA-N 0.000 claims 1
206010030216 Oesophagitis Diseases 0.000 claims 1
108010086019 Secretin Proteins 0.000 claims 1
102100037505 Secretin Human genes 0.000 claims 1
102000006612 Transducin Human genes 0.000 claims 1
108010087042 Transducin Proteins 0.000 claims 1
125000003668 acetyloxy group Chemical group [H]C([H])([H])C(=O)O[*] 0.000 claims 1
235000019631 acid taste sensations Nutrition 0.000 claims 1
WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims 1
210000004913 chyme Anatomy 0.000 claims 1
230000000504 effect on taste Effects 0.000 claims 1
230000006862 enzymatic digestion Effects 0.000 claims 1
208000006881 esophagitis Diseases 0.000 claims 1
235000019197 fats Nutrition 0.000 claims 1
125000003983 fluorenyl group Chemical group C1(=CC=CC=2C3=CC=CC=C3CC12)* 0.000 claims 1
208000018685 gastrointestinal system disease Diseases 0.000 claims 1
239000003102 growth factor Substances 0.000 claims 1
XLYOFNOQVPJJNP-UHFFFAOYSA-M hydroxide Chemical compound [OH-] XLYOFNOQVPJJNP-UHFFFAOYSA-M 0.000 claims 1
230000002473 insulinotropic effect Effects 0.000 claims 1
229940079322 interferon Drugs 0.000 claims 1
210000003292 kidney cell Anatomy 0.000 claims 1
210000000265 leukocyte Anatomy 0.000 claims 1
230000001404 mediated effect Effects 0.000 claims 1
230000001613 neoplastic effect Effects 0.000 claims 1
230000001537 neural effect Effects 0.000 claims 1
230000009984 peri-natal effect Effects 0.000 claims 1
230000008855 peristalsis Effects 0.000 claims 1
125000001436 propyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])[H] 0.000 claims 1
239000002795 scorpion venom Substances 0.000 claims 1
229960002101 secretin Drugs 0.000 claims 1
OWMZNFCDEHGFEP-NFBCVYDUSA-N secretin human Chemical compound C([C@@H](C(=O)N[C@H](C(=O)N[C@@H](CO)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCC(O)=O)C(=O)NCC(=O)N[C@@H](C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(N)=O)C(=O)NCC(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C(C)C)C(N)=O)[C@@H](C)O)NC(=O)[C@@H](NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CO)NC(=O)[C@@H](N)CC=1NC=NC=1)[C@@H](C)O)C1=CC=CC=C1 OWMZNFCDEHGFEP-NFBCVYDUSA-N 0.000 claims 1
230000009758 senescence Effects 0.000 claims 1
210000000952 spleen Anatomy 0.000 claims 1
230000007306 turnover Effects 0.000 claims 1
230000002485 urinary effect Effects 0.000 claims 1
125000000391 vinyl group Chemical group [H]C([*])=C([H])[H] 0.000 claims 1
206010012601 diabetes mellitus Diseases 0.000 abstract description 15
208000030159 metabolic disease Diseases 0.000 abstract description 9
238000010187 selection method Methods 0.000 abstract description 9
230000014860 sensory perception of taste Effects 0.000 abstract description 4
239000003623 enhancer Substances 0.000 abstract description 3
208000010470 Ageusia Diseases 0.000 abstract description 2
230000000903 blocking effect Effects 0.000 abstract description 2
230000030883 sensory perception of salty taste Effects 0.000 abstract 3
206010048685 Oral infection Diseases 0.000 abstract 1
210000001789 adipocyte Anatomy 0.000 abstract 1
235000019611 bitter taste sensations Nutrition 0.000 abstract 1
230000003822 cell turnover Effects 0.000 abstract 1
230000002708 enhancing effect Effects 0.000 abstract 1
230000007365 immunoregulation Effects 0.000 abstract 1
230000030812 sensory perception of bitter taste Effects 0.000 abstract 1
230000030682 sensory perception of sour taste Effects 0.000 abstract 1
230000030738 sensory perception of sweet taste Effects 0.000 abstract 1
230000030688 sensory perception of umami taste Effects 0.000 abstract 1
235000019637 sour taste sensation Nutrition 0.000 abstract 1
235000018102 proteins Nutrition 0.000 description 112
150000007523 nucleic acids Chemical group 0.000 description 74
241000699666 Mus <mouse, genus> Species 0.000 description 62
102000039446 nucleic acids Human genes 0.000 description 53
108020004707 nucleic acids Proteins 0.000 description 53
238000003752 polymerase chain reaction Methods 0.000 description 41
235000001014 amino acid Nutrition 0.000 description 36
229940024606 amino acid Drugs 0.000 description 36
150000001413 amino acids Chemical class 0.000 description 34
210000004379 membrane Anatomy 0.000 description 34
101001032038 Homo sapiens Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4 Proteins 0.000 description 28
102100038718 Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4 Human genes 0.000 description 28
238000002474 experimental method Methods 0.000 description 28
108091028043 Nucleic acid sequence Proteins 0.000 description 20
229940088598 enzyme Drugs 0.000 description 19
230000004907 flux Effects 0.000 description 18
238000009396 hybridization Methods 0.000 description 18
238000003364 immunohistochemistry Methods 0.000 description 17
239000003446 ligand Substances 0.000 description 17
239000013598 vector Substances 0.000 description 17
235000019613 sensory perceptions of taste Nutrition 0.000 description 16
230000035923 taste sensation Effects 0.000 description 16
230000002159 abnormal effect Effects 0.000 description 15
238000004422 calculation algorithm Methods 0.000 description 14
210000003296 saliva Anatomy 0.000 description 14
230000000723 chemosensory effect Effects 0.000 description 13
239000012634 fragment Substances 0.000 description 13
229910052751 metal Inorganic materials 0.000 description 13
239000002184 metal Substances 0.000 description 13
230000008929 regeneration Effects 0.000 description 13
238000011069 regeneration method Methods 0.000 description 13
108020004705 Codon Proteins 0.000 description 12
101000684820 Homo sapiens Sodium channel protein type 3 subunit alpha Proteins 0.000 description 12
102100023720 Sodium channel protein type 3 subunit alpha Human genes 0.000 description 12
239000011148 porous material Substances 0.000 description 12
238000013518 transcription Methods 0.000 description 12
230000035897 transcription Effects 0.000 description 12
125000003275 alpha amino acid group Chemical group 0.000 description 11
239000003795 chemical substances by application Substances 0.000 description 11
239000003112 inhibitor Substances 0.000 description 11
239000013642 negative control Substances 0.000 description 11
239000012190 activator Substances 0.000 description 10
150000001412 amines Chemical class 0.000 description 10
239000011230 binding agent Substances 0.000 description 10
239000000203 mixture Substances 0.000 description 10
208000019454 Feeding and Eating disease Diseases 0.000 description 9
108010004460 Gastric Inhibitory Polypeptide Proteins 0.000 description 9
108010052285 Membrane Proteins Proteins 0.000 description 9
239000012530 fluid Substances 0.000 description 9
239000012071 phase Substances 0.000 description 9
230000035807 sensation Effects 0.000 description 9
235000019615 sensations Nutrition 0.000 description 9
230000001953 sensory effect Effects 0.000 description 9
239000007787 solid Substances 0.000 description 9
208000027559 Appetite disease Diseases 0.000 description 8
230000004913 activation Effects 0.000 description 8
238000001994 activation Methods 0.000 description 8
230000006907 apoptotic process Effects 0.000 description 8
238000000423 cell based assay Methods 0.000 description 8
238000006243 chemical reaction Methods 0.000 description 8
238000004519 manufacturing process Methods 0.000 description 8
108020004999 messenger RNA Proteins 0.000 description 8
206010016807 Fluid retention Diseases 0.000 description 7
102000018697 Membrane Proteins Human genes 0.000 description 7
108091005461 Nucleic proteins Proteins 0.000 description 7
230000003915 cell function Effects 0.000 description 7
208000035475 disorder Diseases 0.000 description 7
239000000796 flavoring agent Substances 0.000 description 7
238000013537 high throughput screening Methods 0.000 description 7
238000005259 measurement Methods 0.000 description 7
239000002773 nucleotide Substances 0.000 description 7
125000003729 nucleotide group Chemical group 0.000 description 7
230000002829 reductive effect Effects 0.000 description 7
238000006467 substitution reaction Methods 0.000 description 7
YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 6
208000023275 Autoimmune disease Diseases 0.000 description 6
206010006895 Cachexia Diseases 0.000 description 6
241000282412 Homo Species 0.000 description 6
150000001768 cations Chemical class 0.000 description 6
230000024245 cell differentiation Effects 0.000 description 6
230000009977 dual effect Effects 0.000 description 6
230000007831 electrophysiology Effects 0.000 description 6
238000002001 electrophysiology Methods 0.000 description 6
238000005516 engineering process Methods 0.000 description 6
239000013604 expression vector Substances 0.000 description 6
235000019634 flavors Nutrition 0.000 description 6
238000013507 mapping Methods 0.000 description 6
229920000642 polymer Polymers 0.000 description 6
210000003370 receptor cell Anatomy 0.000 description 6
239000000758 substrate Substances 0.000 description 6
108091005462 Cation channels Proteins 0.000 description 5
FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 5
241000699670 Mus sp. Species 0.000 description 5
108091034117 Oligonucleotide Proteins 0.000 description 5
241000700159 Rattus Species 0.000 description 5
241000700605 Viruses Species 0.000 description 5
239000005557 antagonist Substances 0.000 description 5
238000003320 cell separation method Methods 0.000 description 5
239000003153 chemical reaction reagent Substances 0.000 description 5
239000002299 complementary DNA Substances 0.000 description 5
231100000433 cytotoxic Toxicity 0.000 description 5
230000001472 cytotoxic effect Effects 0.000 description 5
230000006866 deterioration Effects 0.000 description 5
210000002919 epithelial cell Anatomy 0.000 description 5
210000000981 epithelium Anatomy 0.000 description 5
239000007850 fluorescent dye Substances 0.000 description 5
230000002068 genetic effect Effects 0.000 description 5
210000005260 human cell Anatomy 0.000 description 5
238000000338 in vitro Methods 0.000 description 5
208000027866 inflammatory disease Diseases 0.000 description 5
230000003834 intracellular effect Effects 0.000 description 5
150000002632 lipids Chemical class 0.000 description 5
229930182817 methionine Natural products 0.000 description 5
210000002706 plastid Anatomy 0.000 description 5
238000003908 quality control method Methods 0.000 description 5
238000005204 segregation Methods 0.000 description 5
239000007790 solid phase Substances 0.000 description 5
208000023665 Barrett oesophagus Diseases 0.000 description 4
OBMZMSLWNNWEJA-XNCRXQDQSA-N C1=CC=2C(C[C@@H]3NC(=O)[C@@H](NC(=O)[C@H](NC(=O)N(CC#CCN(CCCC[C@H](NC(=O)[C@@H](CC4=CC=CC=C4)NC3=O)C(=O)N)CC=C)NC(=O)[C@@H](N)C)CC3=CNC4=C3C=CC=C4)C)=CNC=2C=C1 Chemical compound C1=CC=2C(C[C@@H]3NC(=O)[C@@H](NC(=O)[C@H](NC(=O)N(CC#CCN(CCCC[C@H](NC(=O)[C@@H](CC4=CC=CC=C4)NC3=O)C(=O)N)CC=C)NC(=O)[C@@H](N)C)CC3=CNC4=C3C=CC=C4)C)=CNC=2C=C1 OBMZMSLWNNWEJA-XNCRXQDQSA-N 0.000 description 4
108090000994 Catalytic RNA Proteins 0.000 description 4
102000053642 Catalytic RNA Human genes 0.000 description 4
208000015943 Coeliac disease Diseases 0.000 description 4
108020004635 Complementary DNA Proteins 0.000 description 4
108020004414 DNA Proteins 0.000 description 4
ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 4
ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 description 4
241000283973 Oryctolagus cuniculus Species 0.000 description 4
101710176384 Peptide 1 Proteins 0.000 description 4
108010052164 Sodium Channels Proteins 0.000 description 4
FKNQFGJONOIPTF-UHFFFAOYSA-N Sodium cation Chemical compound [Na+] FKNQFGJONOIPTF-UHFFFAOYSA-N 0.000 description 4
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
102000014384 Type C Phospholipases Human genes 0.000 description 4
108010079194 Type C Phospholipases Proteins 0.000 description 4
JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 4
239000000427 antigen Substances 0.000 description 4
108091007433 antigens Proteins 0.000 description 4
102000036639 antigens Human genes 0.000 description 4
210000004369 blood Anatomy 0.000 description 4
239000008280 blood Substances 0.000 description 4
230000036772 blood pressure Effects 0.000 description 4
150000001720 carbohydrates Chemical class 0.000 description 4
235000014633 carbohydrates Nutrition 0.000 description 4
210000000170 cell membrane Anatomy 0.000 description 4
230000001413 cellular effect Effects 0.000 description 4
230000008859 change Effects 0.000 description 4
210000001671 embryonic stem cell Anatomy 0.000 description 4
210000003527 eukaryotic cell Anatomy 0.000 description 4
230000003832 immune regulation Effects 0.000 description 4
230000002055 immunohistochemical effect Effects 0.000 description 4
238000005462 in vivo assay Methods 0.000 description 4
230000001939 inductive effect Effects 0.000 description 4
210000003097 mucus Anatomy 0.000 description 4
108091033319 polynucleotide Proteins 0.000 description 4
102000040430 polynucleotide Human genes 0.000 description 4
230000001737 promoting effect Effects 0.000 description 4
230000004952 protein activity Effects 0.000 description 4
230000005855 radiation Effects 0.000 description 4
230000002285 radioactive effect Effects 0.000 description 4
108091092562 ribozyme Proteins 0.000 description 4
230000035945 sensitivity Effects 0.000 description 4
238000010561 standard procedure Methods 0.000 description 4
108020004463 18S ribosomal RNA Proteins 0.000 description 3
108020005544 Antisense RNA Proteins 0.000 description 3
241000894006 Bacteria Species 0.000 description 3
UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 description 3
206010013911 Dysgeusia Diseases 0.000 description 3
238000002965 ELISA Methods 0.000 description 3
241000238631 Hexapoda Species 0.000 description 3
108060003951 Immunoglobulin Proteins 0.000 description 3
QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 description 3
QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 3
WHXSMMKQMYFTQS-UHFFFAOYSA-N Lithium Chemical compound [Li] WHXSMMKQMYFTQS-UHFFFAOYSA-N 0.000 description 3
235000005135 Micromeria juliana Nutrition 0.000 description 3
240000004808 Saccharomyces cerevisiae Species 0.000 description 3
241000246354 Satureja Species 0.000 description 3
235000007315 Satureja hortensis Nutrition 0.000 description 3
230000003187 abdominal effect Effects 0.000 description 3
235000004279 alanine Nutrition 0.000 description 3
125000000539 amino acid group Chemical group 0.000 description 3
238000010171 animal model Methods 0.000 description 3
238000000137 annealing Methods 0.000 description 3
210000000270 basal cell Anatomy 0.000 description 3
229940049706 benzodiazepine Drugs 0.000 description 3
125000003310 benzodiazepinyl group Chemical class N1N=C(C=CC2=C1C=CC=C2)* 0.000 description 3
230000015572 biosynthetic process Effects 0.000 description 3
235000020958 biotin Nutrition 0.000 description 3
229960002685 biotin Drugs 0.000 description 3
239000011616 biotin Substances 0.000 description 3
230000010261 cell growth Effects 0.000 description 3
230000000295 complement effect Effects 0.000 description 3
239000003184 complementary RNA Substances 0.000 description 3
230000001186 cumulative effect Effects 0.000 description 3
238000005520 cutting process Methods 0.000 description 3
210000000805 cytoplasm Anatomy 0.000 description 3
230000001086 cytosolic effect Effects 0.000 description 3
238000011161 development Methods 0.000 description 3
230000018109 developmental process Effects 0.000 description 3
210000002257 embryonic structure Anatomy 0.000 description 3
GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 3
230000004927 fusion Effects 0.000 description 3
208000021302 gastroesophageal reflux disease Diseases 0.000 description 3
238000012188 high-throughput screening assay Methods 0.000 description 3
238000003018 immunoassay Methods 0.000 description 3
102000018358 immunoglobulin Human genes 0.000 description 3
238000010324 immunological assay Methods 0.000 description 3
238000000099 in vitro assay Methods 0.000 description 3
230000000977 initiatory effect Effects 0.000 description 3
230000003993 interaction Effects 0.000 description 3
229910052744 lithium Inorganic materials 0.000 description 3
210000004072 lung Anatomy 0.000 description 3
230000014759 maintenance of location Effects 0.000 description 3
238000002493 microarray Methods 0.000 description 3
230000035772 mutation Effects 0.000 description 3
210000002445 nipple Anatomy 0.000 description 3
210000004940 nucleus Anatomy 0.000 description 3
230000008520 organization Effects 0.000 description 3
230000000149 penetrating effect Effects 0.000 description 3
239000002157 polynucleotide Substances 0.000 description 3
239000013641 positive control Substances 0.000 description 3
238000002360 preparation method Methods 0.000 description 3
230000004044 response Effects 0.000 description 3
210000003079 salivary gland Anatomy 0.000 description 3
238000003786 synthesis reaction Methods 0.000 description 3
231100000331 toxic Toxicity 0.000 description 3
230000002588 toxic effect Effects 0.000 description 3
238000013519 translation Methods 0.000 description 3
241000701447 unidentified baculovirus Species 0.000 description 3
102000040650 (ribonucleotides)n+m Human genes 0.000 description 2
108010085238 Actins Proteins 0.000 description 2
102000007469 Actins Human genes 0.000 description 2
208000023514 Barrett esophagus Diseases 0.000 description 2
108091026890 Coding region Proteins 0.000 description 2
238000001712 DNA sequencing Methods 0.000 description 2
241000588724 Escherichia coli Species 0.000 description 2
108700039887 Essential Genes Proteins 0.000 description 2
ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 description 2
108091006027 G proteins Proteins 0.000 description 2
102000030782 GTP binding Human genes 0.000 description 2
108091000058 GTP-Binding Proteins 0.000 description 2
WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 2
102100031181 Glyceraldehyde-3-phosphate dehydrogenase Human genes 0.000 description 2
DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 2
108010043121 Green Fluorescent Proteins Proteins 0.000 description 2
102100039215 Guanine nucleotide-binding protein G(t) subunit alpha-3 Human genes 0.000 description 2
206010020772 Hypertension Diseases 0.000 description 2
108700005091 Immunoglobulin Genes Proteins 0.000 description 2
102100034343 Integrase Human genes 0.000 description 2
CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 description 2
241000288906 Primates Species 0.000 description 2
108010092799 RNA-directed DNA polymerase Proteins 0.000 description 2
108700008625 Reporter Genes Proteins 0.000 description 2
208000004337 Salivary Gland Neoplasms Diseases 0.000 description 2
206010061934 Salivary gland cancer Diseases 0.000 description 2
239000004098 Tetracycline Substances 0.000 description 2
108091023040 Transcription factor Proteins 0.000 description 2
102000040945 Transcription factor Human genes 0.000 description 2
QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 2
210000001015 abdomen Anatomy 0.000 description 2
238000002835 absorbance Methods 0.000 description 2
230000009471 action Effects 0.000 description 2
210000004504 adult stem cell Anatomy 0.000 description 2
208000022531 anorexia Diseases 0.000 description 2
235000003704 aspartic acid Nutrition 0.000 description 2
230000001580 bacterial effect Effects 0.000 description 2
230000009286 beneficial effect Effects 0.000 description 2
OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 description 2
235000013361 beverage Nutrition 0.000 description 2
230000003115 biocidal effect Effects 0.000 description 2
230000004071 biological effect Effects 0.000 description 2
230000008827 biological function Effects 0.000 description 2
229920001222 biopolymer Polymers 0.000 description 2
OSGAYBCDTDRGGQ-UHFFFAOYSA-L calcium sulfate Chemical compound [Ca+2].[O-]S([O-])(=O)=O OSGAYBCDTDRGGQ-UHFFFAOYSA-L 0.000 description 2
238000004364 calculation method Methods 0.000 description 2
125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 2
230000009134 cell regulation Effects 0.000 description 2
230000002925 chemical effect Effects 0.000 description 2
230000001684 chronic effect Effects 0.000 description 2
238000004140 cleaning Methods 0.000 description 2
239000013068 control sample Substances 0.000 description 2
230000001351 cycling effect Effects 0.000 description 2
210000000172 cytosol Anatomy 0.000 description 2
238000007405 data analysis Methods 0.000 description 2
230000007423 decrease Effects 0.000 description 2
206010061428 decreased appetite Diseases 0.000 description 2
230000002950 deficient Effects 0.000 description 2
235000013601 eggs Nutrition 0.000 description 2
230000008030 elimination Effects 0.000 description 2
238000003379 elimination reaction Methods 0.000 description 2
108020001507 fusion proteins Proteins 0.000 description 2
102000037865 fusion proteins Human genes 0.000 description 2
239000007789 gas Substances 0.000 description 2
210000005095 gastrointestinal system Anatomy 0.000 description 2
238000003500 gene array Methods 0.000 description 2
210000004602 germ cell Anatomy 0.000 description 2
235000013922 glutamic acid Nutrition 0.000 description 2
239000004220 glutamic acid Substances 0.000 description 2
108020004445 glyceraldehyde-3-phosphate dehydrogenase Proteins 0.000 description 2
230000001339 gustatory effect Effects 0.000 description 2
108010005995 gustducin Proteins 0.000 description 2
238000012203 high throughput assay Methods 0.000 description 2
230000006801 homologous recombination Effects 0.000 description 2
238000002744 homologous recombination Methods 0.000 description 2
230000004941 influx Effects 0.000 description 2
230000005764 inhibitory process Effects 0.000 description 2
210000000936 intestine Anatomy 0.000 description 2
210000003734 kidney Anatomy 0.000 description 2
230000003902 lesion Effects 0.000 description 2
230000004807 localization Effects 0.000 description 2
230000036210 malignancy Effects 0.000 description 2
239000011159 matrix material Substances 0.000 description 2
230000007246 mechanism Effects 0.000 description 2
230000007102 metabolic function Effects 0.000 description 2
MYWUZJCMWCOHBA-VIFPVBQESA-N methamphetamine Chemical compound CN[C@@H](C)CC1=CC=CC=C1 MYWUZJCMWCOHBA-VIFPVBQESA-N 0.000 description 2
239000000178 monomer Substances 0.000 description 2
230000000877 morphologic effect Effects 0.000 description 2
239000002858 neurotransmitter agent Substances 0.000 description 2
239000002777 nucleoside Substances 0.000 description 2
210000000496 pancreas Anatomy 0.000 description 2
230000036961 partial effect Effects 0.000 description 2
230000010287 polarization Effects 0.000 description 2
229920000768 polyamine Polymers 0.000 description 2
210000001236 prokaryotic cell Anatomy 0.000 description 2
238000000159 protein binding assay Methods 0.000 description 2
210000000664 rectum Anatomy 0.000 description 2
108010054624 red fluorescent protein Proteins 0.000 description 2
230000000717 retained effect Effects 0.000 description 2
QZAYGJVTTNCVMB-UHFFFAOYSA-N serotonin Chemical compound C1=C(O)C=C2C(CCN)=CNC2=C1 QZAYGJVTTNCVMB-UHFFFAOYSA-N 0.000 description 2
230000001568 sexual effect Effects 0.000 description 2
239000011780 sodium chloride Substances 0.000 description 2
238000004611 spectroscopical analysis Methods 0.000 description 2
150000003431 steroids Chemical class 0.000 description 2
239000000725 suspension Substances 0.000 description 2
229960002180 tetracycline Drugs 0.000 description 2
229930101283 tetracycline Natural products 0.000 description 2
235000019364 tetracycline Nutrition 0.000 description 2
150000003522 tetracyclines Chemical class 0.000 description 2
101150065732 tir gene Proteins 0.000 description 2
231100000765 toxin Toxicity 0.000 description 2
230000009261 transgenic effect Effects 0.000 description 2
102000035160 transmembrane proteins Human genes 0.000 description 2
108091005703 transmembrane proteins Proteins 0.000 description 2
231100000611 venom Toxicity 0.000 description 2
239000002435 venom Substances 0.000 description 2
210000001048 venom Anatomy 0.000 description 2
230000000007 visual effect Effects 0.000 description 2
238000011179 visual inspection Methods 0.000 description 2
238000005406 washing Methods 0.000 description 2
DIGQNXIGRZPYDK-WKSCXVIASA-N (2R)-6-amino-2-[[2-[[(2S)-2-[[2-[[(2R)-2-[[(2S)-2-[[(2R,3S)-2-[[2-[[(2S)-2-[[2-[[(2S)-2-[[(2S)-2-[[(2R)-2-[[(2S,3S)-2-[[(2R)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[2-[[(2S)-2-[[(2R)-2-[[2-[[2-[[2-[(2-amino-1-hydroxyethylidene)amino]-3-carboxy-1-hydroxypropylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1-hydroxyethylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1,3-dihydroxypropylidene]amino]-1-hydroxyethylidene]amino]-1-hydroxypropylidene]amino]-1,3-dihydroxypropylidene]amino]-1,3-dihydroxypropylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1,3-dihydroxybutylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1-hydroxypropylidene]amino]-1,3-dihydroxypropylidene]amino]-1-hydroxyethylidene]amino]-1,5-dihydroxy-5-iminopentylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1,3-dihydroxybutylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1,3-dihydroxypropylidene]amino]-1-hydroxyethylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1-hydroxyethylidene]amino]hexanoic acid Chemical compound C[C@@H]([C@@H](C(=N[C@@H](CS)C(=N[C@@H](C)C(=N[C@@H](CO)C(=NCC(=N[C@@H](CCC(=N)O)C(=NC(CS)C(=N[C@H]([C@H](C)O)C(=N[C@H](CS)C(=N[C@H](CO)C(=NCC(=N[C@H](CS)C(=NCC(=N[C@H](CCCCN)C(=O)O)O)O)O)O)O)O)O)O)O)O)O)O)O)N=C([C@H](CS)N=C([C@H](CO)N=C([C@H](CO)N=C([C@H](C)N=C(CN=C([C@H](CO)N=C([C@H](CS)N=C(CN=C(C(CS)N=C(C(CC(=O)O)N=C(CN)O)O)O)O)O)O)O)O)O)O)O)O DIGQNXIGRZPYDK-WKSCXVIASA-N 0.000 description 1
DDYAPMZTJAYBOF-ZMYDTDHYSA-N (3S)-4-[[(2S)-1-[[(2S)-1-[[(2S)-5-amino-1-[[(2S)-1-[[(2S)-1-[[(2S)-1-[[(2S)-4-amino-1-[[(2S,3R)-1-[[(2S)-6-amino-1-[[(2S)-1-[[(2S)-4-amino-1-[[(2S)-1-[[(2S)-4-amino-1-[[(2S)-4-amino-1-[[(2S,3S)-1-[[(1S)-1-carboxyethyl]amino]-3-methyl-1-oxopentan-2-yl]amino]-1,4-dioxobutan-2-yl]amino]-1,4-dioxobutan-2-yl]amino]-5-carbamimidamido-1-oxopentan-2-yl]amino]-1,4-dioxobutan-2-yl]amino]-5-carbamimidamido-1-oxopentan-2-yl]amino]-1-oxohexan-2-yl]amino]-3-hydroxy-1-oxobutan-2-yl]amino]-1,4-dioxobutan-2-yl]amino]-4-methylsulfanyl-1-oxobutan-2-yl]amino]-4-methyl-1-oxopentan-2-yl]amino]-3-(1H-indol-3-yl)-1-oxopropan-2-yl]amino]-1,5-dioxopentan-2-yl]amino]-3-methyl-1-oxobutan-2-yl]amino]-1-oxo-3-phenylpropan-2-yl]amino]-3-[[(2S)-5-amino-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-6-amino-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S,3R)-2-[[(2S)-2-[[(2S,3R)-2-[[2-[[(2S)-5-amino-2-[[(2S)-2-[[(2S)-2-amino-3-(1H-imidazol-4-yl)propanoyl]amino]-3-hydroxypropanoyl]amino]-5-oxopentanoyl]amino]acetyl]amino]-3-hydroxybutanoyl]amino]-3-phenylpropanoyl]amino]-3-hydroxybutanoyl]amino]-3-hydroxypropanoyl]amino]-3-carboxypropanoyl]amino]-3-(4-hydroxyphenyl)propanoyl]amino]-3-hydroxypropanoyl]amino]hexanoyl]amino]-3-(4-hydroxyphenyl)propanoyl]amino]-4-methylpentanoyl]amino]-3-carboxypropanoyl]amino]-3-hydroxypropanoyl]amino]-5-carbamimidamidopentanoyl]amino]-5-carbamimidamidopentanoyl]amino]propanoyl]amino]-5-oxopentanoyl]amino]-4-oxobutanoic acid Chemical class [H]N[C@@H](CC1=CNC=N1)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCC(N)=O)C(=O)NCC(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC1=CC=C(O)C=C1)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC1=CC=C(O)C=C1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](C)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC1=CNC2=C1C=CC=C2)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](C)C(O)=O DDYAPMZTJAYBOF-ZMYDTDHYSA-N 0.000 description 1
UKAUYVFTDYCKQA-UHFFFAOYSA-N -2-Amino-4-hydroxybutanoic acid Natural products OC(=O)C(N)CCO UKAUYVFTDYCKQA-UHFFFAOYSA-N 0.000 description 1
VZXTWGWHSMCWGA-UHFFFAOYSA-N 1,3,5-triazine-2,4-diamine Chemical compound NC1=NC=NC(N)=N1 VZXTWGWHSMCWGA-UHFFFAOYSA-N 0.000 description 1
RABZYUWMIDILPP-UHFFFAOYSA-N 1,3-bis(sulfanyl)propane-1,2,3-triol Chemical compound SC(C(C(O)S)O)O RABZYUWMIDILPP-UHFFFAOYSA-N 0.000 description 1
101150029062 15 gene Proteins 0.000 description 1
VGONTNSXDCQUGY-RRKCRQDMSA-N 2'-deoxyinosine Chemical group C1[C@H](O)[C@@H](CO)O[C@H]1N1C(N=CNC2=O)=C2N=C1 VGONTNSXDCQUGY-RRKCRQDMSA-N 0.000 description 1
GOJUJUVQIVIZAV-UHFFFAOYSA-N 2-amino-4,6-dichloropyrimidine-5-carbaldehyde Chemical class NC1=NC(Cl)=C(C=O)C(Cl)=N1 GOJUJUVQIVIZAV-UHFFFAOYSA-N 0.000 description 1
GELVZYOEQVJIRR-UHFFFAOYSA-N 2-chloropyrazine Chemical compound ClC1=CN=CC=N1 GELVZYOEQVJIRR-UHFFFAOYSA-N 0.000 description 1
108020005096 28S Ribosomal RNA Proteins 0.000 description 1
ZOOGRGPOEVQQDX-UUOKFMHZSA-N 3',5'-cyclic GMP Chemical compound C([C@H]1O2)OP(O)(=O)O[C@H]1[C@@H](O)[C@@H]2N1C(N=C(NC2=O)N)=C2N=C1 ZOOGRGPOEVQQDX-UUOKFMHZSA-N 0.000 description 1
101150096316 5 gene Proteins 0.000 description 1
102100022094 Acid-sensing ion channel 2 Human genes 0.000 description 1
206010069754 Acquired gene mutation Diseases 0.000 description 1
108020000948 Antisense Oligonucleotides Proteins 0.000 description 1
101100175474 Arabidopsis thaliana GLP4 gene Proteins 0.000 description 1
XVPZRKIQCKKYNE-UHFFFAOYSA-N Arborine Chemical compound N=1C(=O)C2=CC=CC=C2N(C)C=1CC1=CC=CC=C1 XVPZRKIQCKKYNE-UHFFFAOYSA-N 0.000 description 1
239000004475 Arginine Substances 0.000 description 1
229930192334 Auxin Natural products 0.000 description 1
108090001008 Avidin Proteins 0.000 description 1
241000194110 Bacillus sp. (in: Bacteria) Species 0.000 description 1
108090000363 Bacterial Luciferases Proteins 0.000 description 1
KXDROGADUISDGY-UHFFFAOYSA-N Benzamil hydrochloride Chemical compound C=1C=CC=CC=1CN=C(N)NC(=O)C1=NC(Cl)=C(N)N=C1N KXDROGADUISDGY-UHFFFAOYSA-N 0.000 description 1
241001674044 Blattodea Species 0.000 description 1
241000283690 Bos taurus Species 0.000 description 1
206010006187 Breast cancer Diseases 0.000 description 1
241000208199 Buxus sempervirens Species 0.000 description 1
102000000905 Cadherin Human genes 0.000 description 1
108050007957 Cadherin Proteins 0.000 description 1
241000282472 Canis lupus familiaris Species 0.000 description 1
241000700198 Cavia Species 0.000 description 1
102000034573 Channels Human genes 0.000 description 1
240000001825 Chimonanthus praecox Species 0.000 description 1
108010035563 Chloramphenicol O-acetyltransferase Proteins 0.000 description 1
ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 1
102000010792 Chromogranin A Human genes 0.000 description 1
108010038447 Chromogranin A Proteins 0.000 description 1
241000699800 Cricetinae Species 0.000 description 1
241000938605 Crocodylia Species 0.000 description 1
206010011469 Crying Diseases 0.000 description 1
102000001189 Cyclic Peptides Human genes 0.000 description 1
108010069514 Cyclic Peptides Proteins 0.000 description 1
HMFHBZSHGGEWLO-SOOFDHNKSA-N D-ribofuranose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H]1O HMFHBZSHGGEWLO-SOOFDHNKSA-N 0.000 description 1
230000004544 DNA amplification Effects 0.000 description 1
230000004543 DNA replication Effects 0.000 description 1
108010033806 Degenerin Sodium Channels Proteins 0.000 description 1
YZCKVEUIGOORGS-OUBTZVSYSA-N Deuterium Chemical compound [2H] YZCKVEUIGOORGS-OUBTZVSYSA-N 0.000 description 1
SHIBSTMRCDJXLN-UHFFFAOYSA-N Digoxigenin Natural products C1CC(C2C(C3(C)CCC(O)CC3CC2)CC2O)(O)C2(C)C1C1=CC(=O)OC1 SHIBSTMRCDJXLN-UHFFFAOYSA-N 0.000 description 1
108010016626 Dipeptides Proteins 0.000 description 1
208000030814 Eating disease Diseases 0.000 description 1
UPEZCKBFRMILAV-JNEQICEOSA-N Ecdysone Natural products O=C1[C@H]2[C@@](C)([C@@H]3C([C@@]4(O)[C@@](C)([C@H]([C@H]([C@@H](O)CCC(O)(C)C)C)CC4)CC3)=C1)C[C@H](O)[C@H](O)C2 UPEZCKBFRMILAV-JNEQICEOSA-N 0.000 description 1
102000003837 Epithelial Sodium Channels Human genes 0.000 description 1
108090000140 Epithelial Sodium Channels Proteins 0.000 description 1
208000000461 Esophageal Neoplasms Diseases 0.000 description 1
108700024394 Exon Proteins 0.000 description 1
108090000331 Firefly luciferases Proteins 0.000 description 1
101800001586 Ghrelin Proteins 0.000 description 1
102400000442 Ghrelin-28 Human genes 0.000 description 1
239000004471 Glycine Substances 0.000 description 1
108091005250 Glycophorins Proteins 0.000 description 1
102000004144 Green Fluorescent Proteins Human genes 0.000 description 1
101000659765 Homo sapiens Taste receptor type 1 member 2 Proteins 0.000 description 1
108090000144 Human Proteins Proteins 0.000 description 1
102000003839 Human Proteins Human genes 0.000 description 1
PMMYEEVYMWASQN-DMTCNVIQSA-N Hydroxyproline Chemical compound O[C@H]1CN[C@H](C(O)=O)C1 PMMYEEVYMWASQN-DMTCNVIQSA-N 0.000 description 1
208000001953 Hypotension Diseases 0.000 description 1
102000008394 Immunoglobulin Fragments Human genes 0.000 description 1
108010021625 Immunoglobulin Fragments Proteins 0.000 description 1
108010067060 Immunoglobulin Variable Region Proteins 0.000 description 1
206010062717 Increased upper airway secretion Diseases 0.000 description 1
108090001061 Insulin Proteins 0.000 description 1
UKAUYVFTDYCKQA-VKHMYHEASA-N L-homoserine Chemical compound OC(=O)[C@@H](N)CCO UKAUYVFTDYCKQA-VKHMYHEASA-N 0.000 description 1
AGPKZVBTJJNPAG-WHFBIAKZSA-N L-isoleucine Chemical compound CC[C@H](C)[C@H](N)C(O)=O AGPKZVBTJJNPAG-WHFBIAKZSA-N 0.000 description 1
COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 description 1
OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 1
KZSNJWFQEVHDMF-BYPYZUCNSA-N L-valine Chemical compound CC(C)[C@H](N)C(O)=O KZSNJWFQEVHDMF-BYPYZUCNSA-N 0.000 description 1
108090001090 Lectins Proteins 0.000 description 1
102000004856 Lectins Human genes 0.000 description 1
108060001084 Luciferase Proteins 0.000 description 1
239000005089 Luciferase Substances 0.000 description 1
206010058467 Lung neoplasm malignant Diseases 0.000 description 1
229920000877 Melamine resin Polymers 0.000 description 1
235000006679 Mentha X verticillata Nutrition 0.000 description 1
235000002899 Mentha suaveolens Nutrition 0.000 description 1
235000001636 Mentha x rotundifolia Nutrition 0.000 description 1
102000003792 Metallothionein Human genes 0.000 description 1
108090000157 Metallothionein Proteins 0.000 description 1
241000237852 Mollusca Species 0.000 description 1
208000003445 Mouth Neoplasms Diseases 0.000 description 1
241001529936 Murinae Species 0.000 description 1
UFWIBTONFRDIAS-UHFFFAOYSA-N Naphthalene Chemical compound C1=CC=CC2=CC=CC=C21 UFWIBTONFRDIAS-UHFFFAOYSA-N 0.000 description 1
238000011887 Necropsy Methods 0.000 description 1
229920000784 Nomex Polymers 0.000 description 1
108020004711 Nucleic Acid Probes Proteins 0.000 description 1
102000016979 Other receptors Human genes 0.000 description 1
108090000854 Oxidoreductases Proteins 0.000 description 1
102000004316 Oxidoreductases Human genes 0.000 description 1
238000012408 PCR amplification Methods 0.000 description 1
208000002193 Pain Diseases 0.000 description 1
206010061902 Pancreatic neoplasm Diseases 0.000 description 1
241001631646 Papillomaviridae Species 0.000 description 1
108010043958 Peptoids Proteins 0.000 description 1
241000276498 Pollachius virens Species 0.000 description 1
229920002873 Polyethylenimine Polymers 0.000 description 1
101710182846 Polyhedrin Proteins 0.000 description 1
229920002396 Polyurea Polymers 0.000 description 1
102000004257 Potassium Channel Human genes 0.000 description 1
108020004518 RNA Probes Proteins 0.000 description 1
239000003391 RNA probe Substances 0.000 description 1
230000004570 RNA-binding Effects 0.000 description 1
108020004511 Recombinant DNA Proteins 0.000 description 1
102000007056 Recombinant Fusion Proteins Human genes 0.000 description 1
108010008281 Recombinant Fusion Proteins Proteins 0.000 description 1
PYMYPHUHKUWMLA-LMVFSUKVSA-N Ribose Natural products OC[C@@H](O)[C@@H](O)[C@@H](O)C=O PYMYPHUHKUWMLA-LMVFSUKVSA-N 0.000 description 1
KJTLSVCANCCWHF-UHFFFAOYSA-N Ruthenium Chemical compound [Ru] KJTLSVCANCCWHF-UHFFFAOYSA-N 0.000 description 1
241000607142 Salmonella Species 0.000 description 1
241000612118 Samolus valerandi Species 0.000 description 1
206010039491 Sarcoma Diseases 0.000 description 1
206010039740 Screaming Diseases 0.000 description 1
102000003800 Selectins Human genes 0.000 description 1
108090000184 Selectins Proteins 0.000 description 1
MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 description 1
102000018674 Sodium Channels Human genes 0.000 description 1
208000005718 Stomach Neoplasms Diseases 0.000 description 1
108010090804 Streptavidin Proteins 0.000 description 1
208000006011 Stroke Diseases 0.000 description 1
229930006000 Sucrose Natural products 0.000 description 1
CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
102100035948 Taste receptor type 1 member 2 Human genes 0.000 description 1
241000270666 Testudines Species 0.000 description 1
WKDDRNSBRWANNC-UHFFFAOYSA-N Thienamycin Natural products C1C(SCCN)=C(C(O)=O)N2C(=O)C(C(O)C)C21 WKDDRNSBRWANNC-UHFFFAOYSA-N 0.000 description 1
RYYWUUFWQRZTIU-UHFFFAOYSA-N Thiophosphoric acid Chemical class OP(O)(S)=O RYYWUUFWQRZTIU-UHFFFAOYSA-N 0.000 description 1
206010062129 Tongue neoplasm Diseases 0.000 description 1
208000008312 Tooth Loss Diseases 0.000 description 1
101710120037 Toxin CcdB Proteins 0.000 description 1
102000004338 Transferrin Human genes 0.000 description 1
108090000901 Transferrin Proteins 0.000 description 1
241000209140 Triticum Species 0.000 description 1
235000021307 Triticum Nutrition 0.000 description 1
KZSNJWFQEVHDMF-UHFFFAOYSA-N Valine Natural products CC(C)C(N)C(O)=O KZSNJWFQEVHDMF-UHFFFAOYSA-N 0.000 description 1
108070000030 Viral receptors Proteins 0.000 description 1
208000010399 Wasting Syndrome Diseases 0.000 description 1
230000005856 abnormality Effects 0.000 description 1
150000007513 acids Chemical class 0.000 description 1
239000000853 adhesive Substances 0.000 description 1
230000001070 adhesive effect Effects 0.000 description 1
239000011543 agarose gel Substances 0.000 description 1
235000019666 ageusia Nutrition 0.000 description 1
230000032683 aging Effects 0.000 description 1
HMFHBZSHGGEWLO-UHFFFAOYSA-N alpha-D-Furanose-Ribose Natural products OCC1OC(O)C(O)C1O HMFHBZSHGGEWLO-UHFFFAOYSA-N 0.000 description 1
UPEZCKBFRMILAV-UHFFFAOYSA-N alpha-Ecdysone Natural products C1C(O)C(O)CC2(C)C(CCC3(C(C(C(O)CCC(C)(C)O)C)CCC33O)C)C3=CC(=O)C21 UPEZCKBFRMILAV-UHFFFAOYSA-N 0.000 description 1
230000004075 alteration Effects 0.000 description 1
229910000147 aluminium phosphate Inorganic materials 0.000 description 1
125000003277 amino group Chemical group 0.000 description 1
125000004103 aminoalkyl group Chemical group 0.000 description 1
150000003863 ammonium salts Chemical class 0.000 description 1
229940025131 amylases Drugs 0.000 description 1
239000003242 anti bacterial agent Substances 0.000 description 1
230000009830 antibody antigen interaction Effects 0.000 description 1
230000000890 antigenic effect Effects 0.000 description 1
239000000074 antisense oligonucleotide Substances 0.000 description 1
238000012230 antisense oligonucleotides Methods 0.000 description 1
230000004596 appetite loss Effects 0.000 description 1
238000013459 approach Methods 0.000 description 1
238000009360 aquaculture Methods 0.000 description 1
244000144974 aquaculture Species 0.000 description 1
239000007864 aqueous solution Substances 0.000 description 1
ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 1
238000003149 assay kit Methods 0.000 description 1
235000019606 astringent taste Nutrition 0.000 description 1
239000002363 auxin Substances 0.000 description 1
108010005774 beta-Galactosidase Proteins 0.000 description 1
102000005936 beta-Galactosidase Human genes 0.000 description 1
238000002306 biochemical method Methods 0.000 description 1
230000031018 biological processes and functions Effects 0.000 description 1
239000012472 biological sample Substances 0.000 description 1
239000003131 biological toxin Substances 0.000 description 1
238000001574 biopsy Methods 0.000 description 1
210000000601 blood cell Anatomy 0.000 description 1
210000001124 body fluid Anatomy 0.000 description 1
239000010839 body fluid Substances 0.000 description 1
239000007767 bonding agent Substances 0.000 description 1
230000005587 bubbling Effects 0.000 description 1
125000000484 butyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
239000001175 calcium sulphate Substances 0.000 description 1
235000011132 calcium sulphate Nutrition 0.000 description 1
230000003185 calcium uptake Effects 0.000 description 1
244000309466 calf Species 0.000 description 1
230000023852 carbohydrate metabolic process Effects 0.000 description 1
235000021256 carbohydrate metabolism Nutrition 0.000 description 1
229910052799 carbon Inorganic materials 0.000 description 1
UHBYWPGGCSDKFX-UHFFFAOYSA-N carboxyglutamic acid Chemical compound OC(=O)C(N)CC(C(O)=O)C(O)=O UHBYWPGGCSDKFX-UHFFFAOYSA-N 0.000 description 1
125000002091 cationic group Chemical group 0.000 description 1
230000020411 cell activation Effects 0.000 description 1
210000005056 cell body Anatomy 0.000 description 1
230000030833 cell death Effects 0.000 description 1
239000002771 cell marker Substances 0.000 description 1
230000004663 cell proliferation Effects 0.000 description 1
230000004656 cell transport Effects 0.000 description 1
229920002678 cellulose Polymers 0.000 description 1
239000001913 cellulose Substances 0.000 description 1
238000012512 characterization method Methods 0.000 description 1
239000013043 chemical agent Substances 0.000 description 1
125000003636 chemical group Chemical group 0.000 description 1
239000002561 chemical irritant Substances 0.000 description 1
239000000460 chlorine Substances 0.000 description 1
229910052801 chlorine Inorganic materials 0.000 description 1
210000000349 chromosome Anatomy 0.000 description 1
210000000078 claw Anatomy 0.000 description 1
238000003776 cleavage reaction Methods 0.000 description 1
238000007621 cluster analysis Methods 0.000 description 1
230000004186 co-expression Effects 0.000 description 1
239000011248 coating agent Substances 0.000 description 1
238000000576 coating method Methods 0.000 description 1
206010009887 colitis Diseases 0.000 description 1
230000002860 competitive effect Effects 0.000 description 1
238000003271 compound fluorescence assay Methods 0.000 description 1
210000002808 connective tissue Anatomy 0.000 description 1
230000008878 coupling Effects 0.000 description 1
238000010168 coupling process Methods 0.000 description 1
238000005859 coupling reaction Methods 0.000 description 1
238000004132 cross linking Methods 0.000 description 1
210000004748 cultured cell Anatomy 0.000 description 1
235000021438 curry Nutrition 0.000 description 1
125000004122 cyclic group Chemical group 0.000 description 1
229920005565 cyclic polymer Polymers 0.000 description 1
235000018417 cysteine Nutrition 0.000 description 1
XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 description 1
102000003675 cytokine receptors Human genes 0.000 description 1
108010057085 cytokine receptors Proteins 0.000 description 1
210000005220 cytoplasmic tail Anatomy 0.000 description 1
230000034994 death Effects 0.000 description 1
238000000354 decomposition reaction Methods 0.000 description 1
230000007812 deficiency Effects 0.000 description 1
238000012217 deletion Methods 0.000 description 1
230000037430 deletion Effects 0.000 description 1
238000004925 denaturation Methods 0.000 description 1
230000036425 denaturation Effects 0.000 description 1
208000002925 dental caries Diseases 0.000 description 1
239000005547 deoxyribonucleotide Substances 0.000 description 1
125000002637 deoxyribonucleotide group Chemical class 0.000 description 1
238000000586 desensitisation Methods 0.000 description 1
230000020698 detection of chemical stimulus involved in sensory perception of salty taste Effects 0.000 description 1
238000001784 detoxification Methods 0.000 description 1
229910052805 deuterium Inorganic materials 0.000 description 1
235000014113 dietary fatty acids Nutrition 0.000 description 1
QONQRTHLHBTMGP-UHFFFAOYSA-N digitoxigenin Natural products CC12CCC(C3(CCC(O)CC3CC3)C)C3C11OC1CC2C1=CC(=O)OC1 QONQRTHLHBTMGP-UHFFFAOYSA-N 0.000 description 1
SHIBSTMRCDJXLN-KCZCNTNESA-N digoxigenin Chemical compound C1([C@@H]2[C@@]3([C@@](CC2)(O)[C@H]2[C@@H]([C@@]4(C)CC[C@H](O)C[C@H]4CC2)C[C@H]3O)C)=CC(=O)OC1 SHIBSTMRCDJXLN-KCZCNTNESA-N 0.000 description 1
239000000539 dimer Substances 0.000 description 1
238000006471 dimerization reaction Methods 0.000 description 1
150000002013 dioxins Chemical class 0.000 description 1
235000014632 disordered eating Nutrition 0.000 description 1
229930004069 diterpene Natural products 0.000 description 1
125000000567 diterpene group Chemical group 0.000 description 1
PMMYEEVYMWASQN-UHFFFAOYSA-N dl-hydroxyproline Natural products OC1C[NH2+]C(C([O-])=O)C1 PMMYEEVYMWASQN-UHFFFAOYSA-N 0.000 description 1
238000009509 drug development Methods 0.000 description 1
238000002651 drug therapy Methods 0.000 description 1
235000019564 dysgeusia Nutrition 0.000 description 1
UPEZCKBFRMILAV-JMZLNJERSA-N ecdysone Chemical compound C1[C@@H](O)[C@@H](O)C[C@]2(C)[C@@H](CC[C@@]3([C@@H]([C@@H]([C@H](O)CCC(C)(C)O)C)CC[C@]33O)C)C3=CC(=O)[C@@H]21 UPEZCKBFRMILAV-JMZLNJERSA-N 0.000 description 1
230000005611 electricity Effects 0.000 description 1
238000004520 electroporation Methods 0.000 description 1
210000003158 enteroendocrine cell Anatomy 0.000 description 1
238000001952 enzyme assay Methods 0.000 description 1
201000004101 esophageal cancer Diseases 0.000 description 1
150000002148 esters Chemical class 0.000 description 1
238000011156 evaluation Methods 0.000 description 1
230000007717 exclusion Effects 0.000 description 1
239000004744 fabric Substances 0.000 description 1
229930195729 fatty acid Natural products 0.000 description 1
239000000194 fatty acid Substances 0.000 description 1
150000004665 fatty acids Chemical class 0.000 description 1
210000003608 fece Anatomy 0.000 description 1
210000001559 fluids and secretion Anatomy 0.000 description 1
MHMNJMPURVTYEJ-UHFFFAOYSA-N fluorescein-5-isothiocyanate Chemical compound O1C(=O)C2=CC(N=C=S)=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 MHMNJMPURVTYEJ-UHFFFAOYSA-N 0.000 description 1
238000012921 fluorescence analysis Methods 0.000 description 1
238000000799 fluorescence microscopy Methods 0.000 description 1
108091006047 fluorescent proteins Proteins 0.000 description 1
102000034287 fluorescent proteins Human genes 0.000 description 1
238000009472 formulation Methods 0.000 description 1
239000003205 fragrance Substances 0.000 description 1
206010017758 gastric cancer Diseases 0.000 description 1
230000030135 gastric motility Effects 0.000 description 1
239000003629 gastrointestinal hormone Substances 0.000 description 1
230000036397 gastrointestinal physiology Effects 0.000 description 1
238000003197 gene knockdown Methods 0.000 description 1
238000012239 gene modification Methods 0.000 description 1
230000005017 genetic modification Effects 0.000 description 1
235000013617 genetically modified food Nutrition 0.000 description 1
238000011331 genomic analysis Methods 0.000 description 1
GNKDKYIHGQKHHM-RJKLHVOGSA-N ghrelin Chemical compound C([C@H](NC(=O)[C@@H](NC(=O)[C@H](CO)NC(=O)CN)COC(=O)CCCCCCC)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CO)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC=1N=CNC=1)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N1[C@@H](CCC1)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(N)=O)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCCNC(N)=N)C(O)=O)C1=CC=CC=C1 GNKDKYIHGQKHHM-RJKLHVOGSA-N 0.000 description 1
208000007565 gingivitis Diseases 0.000 description 1
239000011521 glass Substances 0.000 description 1
125000002791 glucosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)[C@H](O1)CO)* 0.000 description 1
150000004676 glycans Chemical class 0.000 description 1
239000005090 green fluorescent protein Substances 0.000 description 1
230000012010 growth Effects 0.000 description 1
210000003128 head Anatomy 0.000 description 1
238000010438 heat treatment Methods 0.000 description 1
230000013632 homeostatic process Effects 0.000 description 1
230000003054 hormonal effect Effects 0.000 description 1
WJRBRSLFGCUECM-UHFFFAOYSA-N hydantoin Chemical compound O=C1CNC(=O)N1 WJRBRSLFGCUECM-UHFFFAOYSA-N 0.000 description 1
229940091173 hydantoin Drugs 0.000 description 1
150000002429 hydrazines Chemical class 0.000 description 1
150000002431 hydrogen Chemical class 0.000 description 1
229910052739 hydrogen Inorganic materials 0.000 description 1
239000001257 hydrogen Substances 0.000 description 1
125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
125000002768 hydroxyalkyl group Chemical group 0.000 description 1
229960002591 hydroxyproline Drugs 0.000 description 1
206010020718 hyperplasia Diseases 0.000 description 1
ZSKVGTPCRGIANV-ZXFLCMHBSA-N imipenem Chemical compound C1C(SCC\N=C\N)=C(C(O)=O)N2C(=O)[C@H]([C@H](O)C)[C@H]21 ZSKVGTPCRGIANV-ZXFLCMHBSA-N 0.000 description 1
229960002182 imipenem Drugs 0.000 description 1
230000003100 immobilizing effect Effects 0.000 description 1
230000002519 immonomodulatory effect Effects 0.000 description 1
230000001900 immune effect Effects 0.000 description 1
229940072221 immunoglobulins Drugs 0.000 description 1
230000001976 improved effect Effects 0.000 description 1
238000011065 in-situ storage Methods 0.000 description 1
238000010348 incorporation Methods 0.000 description 1
SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 description 1
208000015181 infectious disease Diseases 0.000 description 1
230000002401 inhibitory effect Effects 0.000 description 1
102000006495 integrins Human genes 0.000 description 1
108010044426 integrins Proteins 0.000 description 1
208000028774 intestinal disease Diseases 0.000 description 1
210000004347 intestinal mucosa Anatomy 0.000 description 1
102000027411 intracellular receptors Human genes 0.000 description 1
108091008582 intracellular receptors Proteins 0.000 description 1
238000001616 ion spectroscopy Methods 0.000 description 1
229960000310 isoleucine Drugs 0.000 description 1
AGPKZVBTJJNPAG-UHFFFAOYSA-N isoleucine Natural products CCC(C)C(N)C(O)=O AGPKZVBTJJNPAG-UHFFFAOYSA-N 0.000 description 1
238000000370 laser capture micro-dissection Methods 0.000 description 1
150000002611 lead compounds Chemical class 0.000 description 1
239000002523 lectin Substances 0.000 description 1
230000000670 limiting effect Effects 0.000 description 1
208000012987 lip and oral cavity carcinoma Diseases 0.000 description 1
239000002502 liposome Substances 0.000 description 1
239000007788 liquid Substances 0.000 description 1
230000003910 liver physiology Effects 0.000 description 1
208000019017 loss of appetite Diseases 0.000 description 1
235000021266 loss of appetite Nutrition 0.000 description 1
208000012866 low blood pressure Diseases 0.000 description 1
201000005202 lung cancer Diseases 0.000 description 1
208000020816 lung neoplasm Diseases 0.000 description 1
229920002521 macromolecule Polymers 0.000 description 1
208000015486 malignant pancreatic neoplasm Diseases 0.000 description 1
238000002844 melting Methods 0.000 description 1
230000008018 melting Effects 0.000 description 1
239000007769 metal material Substances 0.000 description 1
230000001394 metastastic effect Effects 0.000 description 1
206010061289 metastatic neoplasm Diseases 0.000 description 1
VUZPPFZMUPKLLV-UHFFFAOYSA-N methane;hydrate Chemical compound C.O VUZPPFZMUPKLLV-UHFFFAOYSA-N 0.000 description 1
YACKEPLHDIMKIO-UHFFFAOYSA-N methylphosphonic acid Chemical class CP(O)(O)=O YACKEPLHDIMKIO-UHFFFAOYSA-N 0.000 description 1
244000005700 microbiome Species 0.000 description 1
238000000520 microinjection Methods 0.000 description 1
210000000110 microvilli Anatomy 0.000 description 1
230000005012 migration Effects 0.000 description 1
238000013508 migration Methods 0.000 description 1
235000013336 milk Nutrition 0.000 description 1
210000004080 milk Anatomy 0.000 description 1
239000008267 milk Substances 0.000 description 1
230000004048 modification Effects 0.000 description 1
238000012986 modification Methods 0.000 description 1
108091005601 modified peptides Proteins 0.000 description 1
238000001823 molecular biology technique Methods 0.000 description 1
230000004001 molecular interaction Effects 0.000 description 1
238000002625 monoclonal antibody therapy Methods 0.000 description 1
HDZGCSFEDULWCS-UHFFFAOYSA-N monomethylhydrazine Chemical compound CNN HDZGCSFEDULWCS-UHFFFAOYSA-N 0.000 description 1
230000004899 motility Effects 0.000 description 1
238000010172 mouse model Methods 0.000 description 1
239000003471 mutagenic agent Substances 0.000 description 1
231100000707 mutagenic chemical Toxicity 0.000 description 1
230000003505 mutagenic effect Effects 0.000 description 1
208000010125 myocardial infarction Diseases 0.000 description 1
239000002105 nanoparticle Substances 0.000 description 1
230000007935 neutral effect Effects 0.000 description 1
230000003472 neutralizing effect Effects 0.000 description 1
108010087904 neutravidin Proteins 0.000 description 1
229940127285 new chemical entity Drugs 0.000 description 1
239000004763 nomex Substances 0.000 description 1
238000007899 nucleic acid hybridization Methods 0.000 description 1
239000002853 nucleic acid probe Substances 0.000 description 1
125000003835 nucleoside group Chemical group 0.000 description 1
229920001542 oligosaccharide Polymers 0.000 description 1
150000002482 oligosaccharides Chemical class 0.000 description 1
238000011275 oncology therapy Methods 0.000 description 1
229940005483 opioid analgesics Drugs 0.000 description 1
230000002018 overexpression Effects 0.000 description 1
CELWCAITJAEQNL-UHFFFAOYSA-N oxan-2-ol Chemical compound OC1CCCCO1 CELWCAITJAEQNL-UHFFFAOYSA-N 0.000 description 1
238000010422 painting Methods 0.000 description 1
201000002528 pancreatic cancer Diseases 0.000 description 1
208000008443 pancreatic carcinoma Diseases 0.000 description 1
238000002161 passivation Methods 0.000 description 1
230000037361 pathway Effects 0.000 description 1
208000000689 peptic esophagitis Diseases 0.000 description 1
230000002688 persistence Effects 0.000 description 1
238000002823 phage display Methods 0.000 description 1
239000008194 pharmaceutical composition Substances 0.000 description 1
COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 description 1
208000026435 phlegm Diseases 0.000 description 1
150000003904 phospholipids Chemical class 0.000 description 1
UEZVMMHDMIWARA-UHFFFAOYSA-M phosphonate Chemical compound [O-]P(=O)=O UEZVMMHDMIWARA-UHFFFAOYSA-M 0.000 description 1
XYFCBTPGUUZFHI-UHFFFAOYSA-O phosphonium Chemical compound [PH4+] XYFCBTPGUUZFHI-UHFFFAOYSA-O 0.000 description 1
NBIIXXVUZAFLBC-UHFFFAOYSA-N phosphoric acid Substances OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 1
238000003566 phosphorylation assay Methods 0.000 description 1
125000005498 phthalate group Chemical class 0.000 description 1
XNGIFLGASWRNHJ-UHFFFAOYSA-N phthalic acid Chemical class OC(=O)C1=CC=CC=C1C(O)=O XNGIFLGASWRNHJ-UHFFFAOYSA-N 0.000 description 1
230000000704 physical effect Effects 0.000 description 1
238000000053 physical method Methods 0.000 description 1
229920000412 polyarylene Polymers 0.000 description 1
229920000515 polycarbonate Polymers 0.000 description 1
239000004417 polycarbonate Substances 0.000 description 1
229920000728 polyester Polymers 0.000 description 1
229920000570 polyether Polymers 0.000 description 1
229920001223 polyethylene glycol Polymers 0.000 description 1
229920002098 polyfluorene Polymers 0.000 description 1
229920001282 polysaccharide Polymers 0.000 description 1
239000005017 polysaccharide Substances 0.000 description 1
108020001213 potassium channel Proteins 0.000 description 1
239000000843 powder Substances 0.000 description 1
230000037452 priming Effects 0.000 description 1
230000008569 process Effects 0.000 description 1
230000002250 progressing effect Effects 0.000 description 1
230000000750 progressive effect Effects 0.000 description 1
230000035755 proliferation Effects 0.000 description 1
AAEVYOVXGOFMJO-UHFFFAOYSA-N prometryn Chemical compound CSC1=NC(NC(C)C)=NC(NC(C)C)=N1 AAEVYOVXGOFMJO-UHFFFAOYSA-N 0.000 description 1
230000006916 protein interaction Effects 0.000 description 1
238000001742 protein purification Methods 0.000 description 1
210000001938 protoplast Anatomy 0.000 description 1
JUJWROOIHBZHMG-UHFFFAOYSA-O pyridinium Chemical compound C1=CC=[NH+]C=C1 JUJWROOIHBZHMG-UHFFFAOYSA-O 0.000 description 1
150000003235 pyrrolidines Chemical class 0.000 description 1
238000001959 radiotherapy Methods 0.000 description 1
238000003259 recombinant expression Methods 0.000 description 1
230000001172 regenerating effect Effects 0.000 description 1
230000029865 regulation of blood pressure Effects 0.000 description 1
230000018406 regulation of metabolic process Effects 0.000 description 1
238000011160 research Methods 0.000 description 1
230000001177 retroviral effect Effects 0.000 description 1
238000012552 review Methods 0.000 description 1
239000002336 ribonucleotide Substances 0.000 description 1
108020004418 ribosomal RNA Proteins 0.000 description 1
229910052707 ruthenium Inorganic materials 0.000 description 1
235000002020 sage Nutrition 0.000 description 1
230000003248 secreting effect Effects 0.000 description 1
210000004739 secretory vesicle Anatomy 0.000 description 1
238000000926 separation method Methods 0.000 description 1
238000002864 sequence alignment Methods 0.000 description 1
229940076279 serotonin Drugs 0.000 description 1
208000027390 severe congenital neutropenia 3 Diseases 0.000 description 1
230000019491 signal transduction Effects 0.000 description 1
230000011664 signaling Effects 0.000 description 1
239000002924 silencing RNA Substances 0.000 description 1
150000003384 small molecules Chemical class 0.000 description 1
238000003746 solid phase reaction Methods 0.000 description 1
238000010532 solid phase synthesis reaction Methods 0.000 description 1
238000010671 solid-state reaction Methods 0.000 description 1
239000000243 solution Substances 0.000 description 1
230000037439 somatic mutation Effects 0.000 description 1
230000009870 specific binding Effects 0.000 description 1
210000003594 spinal ganglia Anatomy 0.000 description 1
235000015096 spirit Nutrition 0.000 description 1
230000037351 starvation Effects 0.000 description 1
201000011549 stomach cancer Diseases 0.000 description 1
239000005720 sucrose Substances 0.000 description 1
235000000346 sugar Nutrition 0.000 description 1
150000008163 sugars Chemical class 0.000 description 1
229920001059 synthetic polymer Polymers 0.000 description 1
238000012353 t test Methods 0.000 description 1
230000036327 taste response Effects 0.000 description 1
230000002123 temporal effect Effects 0.000 description 1
208000001608 teratocarcinoma Diseases 0.000 description 1
150000003505 terpenes Chemical class 0.000 description 1
238000010998 test method Methods 0.000 description 1
150000003527 tetrahydropyrans Chemical class 0.000 description 1
NEUOBESLMIKJSB-UHFFFAOYSA-J tetrasodium;tetraacetate Chemical compound [Na+].[Na+].[Na+].[Na+].CC([O-])=O.CC([O-])=O.CC([O-])=O.CC([O-])=O NEUOBESLMIKJSB-UHFFFAOYSA-J 0.000 description 1
238000002560 therapeutic procedure Methods 0.000 description 1
150000003548 thiazolidines Chemical class 0.000 description 1
150000003568 thioethers Chemical class 0.000 description 1
150000003573 thiols Chemical class 0.000 description 1
210000001541 thymus gland Anatomy 0.000 description 1
239000005495 thyroid hormone Substances 0.000 description 1
229940036555 thyroid hormone Drugs 0.000 description 1
230000001550 time effect Effects 0.000 description 1
201000006134 tongue cancer Diseases 0.000 description 1
230000000699 topical effect Effects 0.000 description 1
239000003053 toxin Substances 0.000 description 1
108700012359 toxins Proteins 0.000 description 1
238000012549 training Methods 0.000 description 1
FGMPLJWBKKVCDB-UHFFFAOYSA-N trans-L-hydroxy-proline Natural products ON1CCCC1C(O)=O FGMPLJWBKKVCDB-UHFFFAOYSA-N 0.000 description 1
230000005030 transcription termination Effects 0.000 description 1
239000012581 transferrin Substances 0.000 description 1
230000009466 transformation Effects 0.000 description 1
238000011830 transgenic mouse model Methods 0.000 description 1
230000014616 translation Effects 0.000 description 1
230000001960 triggered effect Effects 0.000 description 1
OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 1
241001430294 unidentified retrovirus Species 0.000 description 1
239000004474 valine Substances 0.000 description 1
235000013311 vegetables Nutrition 0.000 description 1
108700026220 vif Genes Proteins 0.000 description 1
239000013603 viral vector Substances 0.000 description 1
230000003612 virological effect Effects 0.000 description 1
235000019166 vitamin D Nutrition 0.000 description 1
239000011710 vitamin D Substances 0.000 description 1
238000001262 western blot Methods 0.000 description 1
210000004885 white matter Anatomy 0.000 description 1