TW200517398A - Method for recombinant protein purification, modification and immobilization - Google Patents
Method for recombinant protein purification, modification and immobilizationInfo
- Publication number
- TW200517398A TW200517398A TW092132715A TW92132715A TW200517398A TW 200517398 A TW200517398 A TW 200517398A TW 092132715 A TW092132715 A TW 092132715A TW 92132715 A TW92132715 A TW 92132715A TW 200517398 A TW200517398 A TW 200517398A
- Authority
- TW
- Taiwan
- Prior art keywords
- recombinant protein
- immobilization
- modification
- protein purification
- purifying
- Prior art date
Links
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 title abstract 7
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 title abstract 7
- 238000000034 method Methods 0.000 title abstract 4
- 238000012986 modification Methods 0.000 title abstract 2
- 230000004048 modification Effects 0.000 title abstract 2
- 238000001742 protein purification Methods 0.000 title 1
- 239000003153 chemical reaction reagent Substances 0.000 abstract 2
- 230000003100 immobilizing effect Effects 0.000 abstract 2
- 238000000746 purification Methods 0.000 abstract 2
- 238000000502 dialysis Methods 0.000 abstract 1
- 238000010353 genetic engineering Methods 0.000 abstract 1
- 239000002808 molecular sieve Substances 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 238000011084 recovery Methods 0.000 abstract 1
- URGAHOPLAPQHLN-UHFFFAOYSA-N sodium aluminosilicate Chemical compound [Na+].[Al+3].[O-][Si]([O-])=O.[O-][Si]([O-])=O URGAHOPLAPQHLN-UHFFFAOYSA-N 0.000 abstract 1
- 239000000758 substrate Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/78—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Toxicology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Peptides Or Proteins (AREA)
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| TW092132715A TW200517398A (en) | 2003-11-21 | 2003-11-21 | Method for recombinant protein purification, modification and immobilization |
| US10/798,440 US20050112603A1 (en) | 2003-11-21 | 2004-03-12 | Method for purification, modification and immobilization of recombinant protein |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| TW092132715A TW200517398A (en) | 2003-11-21 | 2003-11-21 | Method for recombinant protein purification, modification and immobilization |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| TW200517398A true TW200517398A (en) | 2005-06-01 |
Family
ID=34588372
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| TW092132715A TW200517398A (en) | 2003-11-21 | 2003-11-21 | Method for recombinant protein purification, modification and immobilization |
Country Status (2)
| Country | Link |
|---|---|
| US (1) | US20050112603A1 (zh) |
| TW (1) | TW200517398A (zh) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111417404A (zh) * | 2017-09-28 | 2020-07-14 | 格尔托公司 | 重组胶原蛋白和弹性蛋白分子及其用途 |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2319061B1 (es) * | 2007-09-11 | 2010-02-10 | Biomedal, S.L. | Metodo de conservacion de peptidos o proteinas. |
| US8222206B2 (en) | 2008-03-04 | 2012-07-17 | Taiwan Advance Bio-Pharm Inc. | Method of enhancing the mobilization of hematopoietic stem cells using TAT-HOXB4H |
| KR100973428B1 (ko) * | 2008-05-19 | 2010-08-03 | 한국과학기술연구원 | 말토오스 결합 단백질을 이용한 생리활성 폴리펩타이드의고정화 방법 |
| KR20100063579A (ko) * | 2008-12-03 | 2010-06-11 | 한국생명공학연구원 | 프로모터 변이체 및 이를 이용한 단백질 생산방법 |
| EP3769083A1 (en) | 2018-03-21 | 2021-01-27 | Waters Technologies Corporation | Non-antibody high-affinity-based sample preparation, sorbents, devices and methods |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6150123A (en) * | 1998-09-03 | 2000-11-21 | Centre Integre De Recherches Biocliniques Sur Le Sida (Cirbs) | Affinity biotinylation |
| US20020102617A1 (en) * | 2000-08-03 | 2002-08-01 | Macbeath Gavin | Protein microarrays |
-
2003
- 2003-11-21 TW TW092132715A patent/TW200517398A/zh unknown
-
2004
- 2004-03-12 US US10/798,440 patent/US20050112603A1/en not_active Abandoned
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111417404A (zh) * | 2017-09-28 | 2020-07-14 | 格尔托公司 | 重组胶原蛋白和弹性蛋白分子及其用途 |
Also Published As
| Publication number | Publication date |
|---|---|
| US20050112603A1 (en) | 2005-05-26 |
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