TR201818506T4 - Transgeni̇k faktör vii&#8217#&ni̇n saflaştirilmasina yöneli̇k yöntem. - Google Patents

Transgeni̇k faktör vii&#8217#&ni̇n saflaştirilmasina yöneli̇k yöntem. Download PDF

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Publication number
TR201818506T4
TR201818506T4 TR2018/18506T TR201818506T TR201818506T4 TR 201818506 T4 TR201818506 T4 TR 201818506T4 TR 2018/18506 T TR2018/18506 T TR 2018/18506T TR 201818506 T TR201818506 T TR 201818506T TR 201818506 T4 TR201818506 T4 TR 201818506T4
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TR
Turkey
Prior art keywords
factor vii
affinity ligand
transgenic factor
fvii
purifying
Prior art date
Application number
TR2018/18506T
Other languages
English (en)
Inventor
Bataille Damien
Nogre Michel
Sami Chtourou Abdessatar
Original Assignee
Lab Francais Du Fractionnement
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lab Francais Du Fractionnement filed Critical Lab Francais Du Fractionnement
Publication of TR201818506T4 publication Critical patent/TR201818506T4/tr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/745—Blood coagulation or fibrinolysis factors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/36—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against blood coagulation factors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6437—Coagulation factor VIIa (3.4.21.21)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/22—Immunoglobulins specific features characterized by taxonomic origin from camelids, e.g. camel, llama or dromedary
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C07K2317/569—Single domain, e.g. dAb, sdAb, VHH, VNAR or nanobody®
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21021—Coagulation factor VIIa (3.4.21.21)

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Cell Biology (AREA)
  • Toxicology (AREA)
  • Food Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Mevcut buluş, transgenik kaynaklardan rekombinant insan aktifleştirilmiş Faktör VII&#8217#&nin saflaştırılmasına yönelik belirli olarak kullanışlı olan bir anti-Faktör VII afinite ligandı ile ilgilidir. Diğer ortogonal kromatografik adımları ile kombine edilmiş afinite ligandı, düşük yüzdede degrade veya oksitlenmiş FVII formuna sahip, agrega içermeyen, tamamen aktifleştirilmiş, yüksek derecede saflaştırılmış FVII solüsyonunun hazırlanmasına olanak verir.
TR2018/18506T 2012-07-19 2013-07-18 Transgeni̇k faktör vii&#8217#&ni̇n saflaştirilmasina yöneli̇k yöntem. TR201818506T4 (tr)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP12305882.8A EP2687595B1 (en) 2012-07-19 2012-07-19 Method for purifying transgenic factor VII

Publications (1)

Publication Number Publication Date
TR201818506T4 true TR201818506T4 (tr) 2019-01-21

Family

ID=48856605

Family Applications (1)

Application Number Title Priority Date Filing Date
TR2018/18506T TR201818506T4 (tr) 2012-07-19 2013-07-18 Transgeni̇k faktör vii&#8217#&ni̇n saflaştirilmasina yöneli̇k yöntem.

Country Status (19)

Country Link
US (1) US9982247B2 (tr)
EP (2) EP2687595B1 (tr)
JP (1) JP6219947B2 (tr)
KR (2) KR20140129015A (tr)
CN (1) CN104640980B (tr)
AR (1) AR091836A1 (tr)
AU (1) AU2013291969B2 (tr)
CA (1) CA2879109C (tr)
DK (1) DK2875129T3 (tr)
ES (1) ES2703541T3 (tr)
IL (1) IL236755B (tr)
MX (1) MX358139B (tr)
MY (1) MY172995A (tr)
PH (1) PH12015500083A1 (tr)
PL (1) PL2875129T4 (tr)
SG (1) SG11201500254PA (tr)
TR (1) TR201818506T4 (tr)
TW (1) TWI531576B (tr)
WO (1) WO2014013024A1 (tr)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1890257A (zh) 2003-12-01 2007-01-03 诺和诺德医疗保健公司 液体因子ⅶ组合物的病毒过滤
WO2012090067A1 (en) 2010-12-30 2012-07-05 Lfb Biotechnologies Glycols as pathogen inactivating agents
FR3006591B1 (fr) 2013-06-11 2016-05-06 Lab Francais Du Fractionnement Composition de facteur vii presentant un point isoelectrique substantiellement homogene
FR3082427B1 (fr) * 2018-06-14 2020-09-25 Lab Francais Du Fractionnement Combinaison de facteur vii et d'un anticorps bispecifique anti-facteurs ix et x
CN114438061B (zh) * 2020-10-30 2023-10-20 北京双鹭立生医药科技有限公司 用于纯化凝血因子vii的方法

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GR860984B (en) 1985-04-17 1986-08-18 Zymogenetics Inc Expression of factor vii and ix activities in mammalian cells
FR2632524B1 (fr) 1988-06-09 1992-03-13 Fondation Nale Transfusion San Procede de preparation d'une fraction concentree en facteur viia et son application a titre de medicament
FR2677652B1 (fr) 1991-06-12 2005-05-27 Agronomique Inst Nat Rech Procede de preparation d'une proteine d'interet dans le lait d'un animal transgenique, produit obtenu et cellule eucaryote utilisee.
FR2684999A1 (fr) 1991-12-16 1993-06-18 Aquitaine Dev Transf Sanguine Procede de fabrication d'un concentre de facteur vii active de haute purete essentiellement depourvu des facteurs vitamine k dependants et des facteurs viiic et viiicag.
EP2192131A1 (en) 1992-08-21 2010-06-02 Vrije Universiteit Brussel Immunoglobulins devoid of light chains
DK38293D0 (da) 1993-03-31 1993-03-31 Novo Nordisk As Fremstilling af proteiner
US6838254B1 (en) * 1993-04-29 2005-01-04 Conopco, Inc. Production of antibodies or (functionalized) fragments thereof derived from heavy chain immunoglobulins of camelidae
US6268487B1 (en) 1996-05-13 2001-07-31 Genzyme Transgenics Corporation Purification of biologically active peptides from milk
TWI268933B (en) 2003-06-10 2006-12-21 Animal Technology Inst Taiwan Method for separating protein from animal milk
FR2901707B1 (fr) 2006-05-31 2017-09-29 Lab Francais Du Fractionnement Composition de facteur vii recombinant ou transgenique, chaque molecule de facteur vii possedant deux sites de n-glycosylation a motifs glycanniques definis
FR2901796A1 (fr) 2006-05-31 2007-12-07 Lab Francais Du Fractionnement Procede d'extraction d'une ou de plusieurs proteines presentes dans du lait
FR2910786B1 (fr) 2006-12-29 2017-08-11 Laboratoire Francais Du Fractionnement Et Des Biotechnologies (Lfb) "procede d'extraction d'une proteine presente dans du lait"
BRPI0822557A2 (pt) 2008-03-25 2019-09-24 Bioprotein Tech Sa coelhos transgênicos produtores de fator vii humano
WO2009141418A1 (en) 2008-05-23 2009-11-26 Novo Nordisk A/S Formulations of peg-functionalised serine proteases with high concentrations of an aromatic preservative
FR2942233B1 (fr) 2009-02-19 2015-03-13 Lfb Biotechnologies Moyens pour la purification d'une proteine du plasma sanguin, et procedes pour sa mise en oeuvre
FR2947181B1 (fr) 2009-06-26 2012-05-04 Lfb Biotechnologies Composition de facteur vii
CN102161701A (zh) * 2011-01-28 2011-08-24 上海兴迪金生物技术有限公司 从细胞培养液或血浆组分中分离纯化高纯度活化凝血七因子的方法

Also Published As

Publication number Publication date
JP6219947B2 (ja) 2017-10-25
AU2013291969A1 (en) 2015-02-05
EP2687595B1 (en) 2018-05-30
MX2015000778A (es) 2015-10-14
US20150175983A1 (en) 2015-06-25
AR091836A1 (es) 2015-03-04
KR20170083166A (ko) 2017-07-17
PL2875129T3 (pl) 2019-03-29
ES2703541T3 (es) 2019-03-11
EP2875129B1 (en) 2018-09-05
US9982247B2 (en) 2018-05-29
KR20140129015A (ko) 2014-11-06
AU2013291969B2 (en) 2018-07-12
TW201416373A (zh) 2014-05-01
JP2015522600A (ja) 2015-08-06
CA2879109A1 (en) 2014-01-23
CN104640980A (zh) 2015-05-20
DK2875129T3 (en) 2019-01-07
PH12015500083A1 (en) 2015-03-02
EP2687595A1 (en) 2014-01-22
WO2014013024A1 (en) 2014-01-23
PL2875129T4 (pl) 2019-03-29
SG11201500254PA (en) 2015-02-27
EP2875129A1 (en) 2015-05-27
MY172995A (en) 2019-12-18
KR101842412B1 (ko) 2018-03-26
TWI531576B (zh) 2016-05-01
CN104640980B (zh) 2019-01-08
CA2879109C (en) 2019-03-05
MX358139B (es) 2018-08-06
IL236755B (en) 2018-03-29

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