TR201806407A2 - Natural Blend Containing Chitosan and Rosemary Oil Developed to Fight Red Mite - Google Patents

Abstract

The invention was developed to remove the parasite known as the winged red mite (dermanyssus gallinae) or shortly the red mite, which causes deterioration of animal welfare in poultry breeding, chitosan (a linear aminopolysaccharide obtained by deacetylation of chitin) and rosemary (Rosmarinus officinalis L). .) relates to the natural mixture containing oil.

Description

DESCRIPTION Developed to Fight Red Mite Natural Mixture Containing Chitosan and Rosemary Oil Technical Area The invention is used in poultry farming, which causes deterioration of animal welfare. the parasite known as the red mite (dermanyssus gallinae) or the red mite for short, Developed to remove chitosan (obtained by deacetylation of chitin) a linear aminopolysaccharide) and rosemary (Rosmarinus officinalis L.) oil. it's about karma.

State of the Art Dermanyssus gallinae, winged red mite or red mite for short, negative It is a foreign language, the importance of which is grasped day by day in poultry breeding due to its effects. is a parasite. The effects of this blood-fed external parasite on health and yield in animals. has many effects. The acarine in question has negative effects on animal welfare. In addition to its effects, the issue also has a serious dimension in terms of public health.

It is known that its existence is reported all over the world, especially in Europe, and its name is frequently mentioned. Measures against parasites are relatively few in our country. Raised up internal parasites Although it has not been introduced, the problem of external parasites in poultry breeding, especially in recent years With the increase in alternative growing systems such as field cultivation and organic cultivation, together become more visible. Dermanyssus gallinae, whose main host is poultry is the most important of them.

Dermanyssus gallinae, also called the red-winged mite, or the red mite for short It is a foreign language, the importance of which is understood day by day in poultry breeding due to its negative effects. is a parasite. Although it is very new as a problem in industrial aquaculture, it is a big problem. This external parasite, which can be seen frequently in other small-scale breeding systems, is a mite type. Some of the breeders are not aware of its existence, This mite species, which most of them describe as lice/insect, can be seen in various breeding systems. Turkey's climate zone and other In the context of its geographical features, it is, so to speak, a parasite paradise. In this sense Turkey is also a very suitable geography for the red-winged mite.

Dermanyssus gallinae is the host in terms of its mouth structure and diet. It is a blood-feeding dental parasite. This means both blood-related health on the host. problems, as well as health problems related to the diet of the mite. causes adverse effects. Dermanyssus gallinae has a mouth structure and It creates a serious pressure on the host due to its feeding habits. Promise The pressure in question is the skin of the mites on the host to suck blood. It occurs due to the severe bite they cause on them. Red The penetrating-sucking mouth structure of the mite, various types of skin surface where it sucks blood. can cause many skin problems, from rashes to severe inflammations and dermatitis. can open. These 'bites' are usually caused by a parasite that is active at night. realization of this, shows how uncomfortable the host can be even during resting time. shows. In this context, the problem caused by mites is only related to anemia. uneasiness, itching, restlessness and irritation on animals. As well as different symptoms of infestation can be seen.

The biological mechanism of the infested host is to survive rather than yield. The fact that it enters a movement to stay put is only due to low productivity. does not show. Parasites also affect the quality of animal products. also cause a decrease. The high density of red mite populations Eggs as a result of crushing blood-sucking mites in cage systems It is known that caking occurs on the bark. in animal production parasites do not only affect the animal, but also affect human, that is, public health. There is also an influencing dimension. Except for parasites specific to one species, the presence of parasite species that can infect more than one species, may cause it to occur. Especially living with people Such a parasite transmission from animal species to humans is a public health concern. is important.

The production of poultry meat and other poultry products is increasing in parallel with the increasing population and consumption. has been increasing. Accordingly, the unit shaped only by economic concerns and Production systems aiming at high production have been developed in the field. However, the animal by animal rights advocates of these systems that ignore the welfare of criticism of the consumers' demands for village eggs or backyard chickens. increase brought the development and dissemination of new systems to the agenda.

In the patent research about the known state of the technique, KR numbered “COMPOSlTION FOR ATTRACTING RED MlTE AND lNSECT TRAP A patent document titled "COMPRILSING THE SAME" has been found. Said calcium carbonate, known in the patent document as fossilized coral stone Harm to animals by attracting red mites with a mixture containing (CaCog) A system for trapping parasites is described. Said essential substance in the document enabling it to fulfill the objectives of the invention. It was stated that Cacog, Conchiolin and aragonite found in coral stone.

The shells of oysters are quite resistant. Oysters have sand, gravel or damage to their insides. When parasitic organisms enter, they are disturbed and the method of protection is They insulate this intruder and overlay it with the “aragonite” that makes up the pearl. They start coating with (CaCO3) and “Conchiolin” which acts as a glue. Meet In fact, it is claimed that the information in many articles is new. It is a print made. The difficulty of obtaining the mentioned substances Considering the costly and environmental damage of the necessary processes in order to be able to in order to remove the parasite known as the red mite, The need for products obtained from natural mixtures to be used is quite high.

Brief Description of the Invention The invention is used in poultry farming, which causes deterioration of animal welfare. the parasite known as the red mite (dermanyssus gallinae) or the red mite for short, Developed to remove chitosan (obtained by deacetylation of chitin) a linear aminopolysaccharide) and rosemary (Rosmarinus officinalis L.) oil. it's about karma.

The aim of the invention is to replace chemical pesticides used in the fight against red mites. to develop alternative natural antimicrobial agents, thereby increasing the impact on animal welfare. Removal of red mite, which has adverse effects, from poultry. is to provide.

The aim of the invention is to improve the productivity of poultry. is to provide a solution with natural mixture as an alternative to chemicals.

The aim of the invention is to treat anemia caused by mites on poultry, different types of infestation, such as restlessness, itching, restlessness, and irritation. to prevent symptoms.

Detailed Description of the Invention In this detailed explanation, it is necessary to remove the parasite known as the red mite for short. Chitosan (a linear product obtained by deacetylation of chitin) was developed for the purpose of aminopolysaccharide) and rosemary (Rosmarinus officinalis L.) oil only as an example for a better understanding of the subject and no limiting effect It is described in a way that does not constitute.

The red mite is a blood-feeding external parasite and its main host is poultry. especially chickens. In addition, the parasite in question was also seen in turkey coops. It is also known to infest cage birds, pigeons and wild birds. Red Unlike many external parasites, the mite is usually active at night, in the dark. It is a species found on the host. that red mites feed at night, at other times, in the cages, walls, or nearby cracks and crevices. It is known that in By the host in relation to the red mite Determining the location of the host by some semiochemicals secreted and called kairomone It is known to be effective in A similar situation exists in plants. that some substances secreted by plants have a chiromonal effect for parasites. known. These kairomones, which are effective on the behavior of parasites, It has effects such as attracting, stopping, slowing and encouraging.

Based on the aforementioned information, the improvement of animal welfare in poultry breeding parasite, known as red mite, which causes spoilage, from poultry Developed to remove chitosan (obtained by deacetylation of chitin) a linear aminopolysaccharide) and rosemary (Rosmarinus officinalis L.) oil. the mixture is prepared.

In the most basic form for the preparation of the mentioned mixture; . Preparation of acetic acid solution o Adding powder chitosan to acetic acid solution, o Adding glycerol to the solution containing acetic acid and chitosan, o Precipitation of the solution by centrifugation o Adding rosemary oil and tween 80 to the mixture o Homogenization of the mixture The process steps are being implemented.

Said mix content, preferred percentages and usable percentages rates are shown in Table-1.

Table-1: Natural mixture content developed to combat red mite Element Preferred [%1 Available[%] 1. Powder Chitosan 0.5 0.1-1 2. Acetic Acid 2,5 2-3 3. Glycerol 0.7 0.1-1 4. Tween 80 0.5 0.1-1 . Rosemary Oil 1 0.5-2 6. Pure Water 94.8 80-150 The powder chitosan structure used in the mixture content described in Table-1 is poly-[ß-(1,4)-2- amino-2-deoxy-ß-D-glucopyranose]. Powder chitosan of the mixture subject to the invention biocompatibility, antimicrobial activity, composite with other materials It is characterized by its ability to form, to allow cellular attachment and proliferation. therefore preferred. Powder chitosan is easily soluble in acetic acid.

Glycerol, also known as glycerin, belonging to the subject mixture of the invention is sweet, odorless and colorless. is a liquid. It is found naturally in animal and vegetable oils. subject of the invention Tween 80, another element used in the mixture, is also used as polysorbate 80. known, its chemical formula is C32H60010. Another element used in the mixture rosemary oil, steam distillation from rosemary (Rosmarinus officinalis L.) plant obtained by the method. In steam distillation method, dried 200-400 grams The rosemary plant is placed in the plant reservoir of the clevenger system and 2 liters of water is placed on it. was added and distilled for 2 hours at the boiling temperature of the water. Distillation Accumulation of volatile oil by removing the water collected at the bottom in the condensation environment during the has been provided. Obtained essential oils were put into 1.5 ml eppendorf tubes and lighted. tightly wrapped with aluminum foil and stored in the refrigerator at +4°C. has been preserved. camphene, 6% ß-pinene 5% borneol, 5% myrcene, 3% bornyl acetate, 2% d-terpineol.

Rosemary (Rosmarinus officinalis L.) from the Laminacae (Labiatae) family is an important It is a type of medicinal and aromatic plant. In our country, it is also called with different names (kusdili, hasalbal and rosemary, named as apurene), 50-100 cm high, bushy in appearance, leaves in winter It is an evergreen perennial herb with pale blue flowers.

The acetic acid solution applied to obtain the mixture of the invention In the preparation step, 1% v/v, 40 ml acetic acid solution in distilled water is added. by using a magnetic stirrer at 60°C for 30 minutes. mixed. Then, adding powder chitosan in acetic acid solution is done. In the first step, 0.5% w/v chitosan was added to the acetic acid solution, under the same conditions. at a temperature of 60°C until the chitosan melts completely and a homogeneous mixture is obtained. minutes stirring was continued. Then 0.7% v/h for viscosity reduction Glycerol was added and mixed in a magnetic stirrer at 50°C for 6 hours. of the solution in the centrifugal sedimentation step, the insoluble porticoli in the solution Centrifuge at 5000 rpm for 5 minutes in a 50 ml centrifuge tube for removal. action is applied. The process of adding rosemary oil and tween 80 to the mixture After the mixture is mixed in the magnetic stirrer for another 10 minutes, pre-prepared rosemary oil- Tween 80 (1:05 v/h) mixture, target chitosan added to the solution in drops. Mixture homogenization process In the next step, until the mixture becomes a homogeneous emulsion, it is left in the room for an average of 1.5 hours. It was continued to be mixed at the temperature. Finally obtained nanoparticles by taking it into the centrifuge tube and ensuring that the caps of the tubes are not airtight. It was paraffinized and stored at +4°C until use.

Red mites are scattering and scattering in environments with poultry during the day. they hide in the cracks. At night, they come out of their hiding places and come to the host animal and they suck blood. It is enough for them to stay on the birds for 1-2 hours to feed. new The blood-sucking lice are bright red in color, once the blood has been digested, their color they gradually darken and eventually become colorless. Based on this information In order to combat the red mite by exiting, the mite infestation density should be increased. Two pieces of walnut wood were used as traps for estimation and monitoring. wooden boards are used. Slab size on each wooden board 2 channels with a width of 3 mm were opened in the direction of along the plate length There are also 4 pieces of 3 mm wide in the most straight line of the plates with channels. the channel is opened. The wooden boards prepared in this way, the hollow channels, the interior and they are paired as a pair on top of each other. to one of these, Screwing is done in order to adjust the height between the traps. In this way, each It is ensured that a certain gap remains between the wooden boards that are closed on it.

The mixture mentioned in order to observe the effects of the mixture which is the subject of the invention is walnut. winged animal It is positioned in a cage used in breeding. wooden boards for a while in order to determine the mite density after being kept in the cage. no mites were found in the plate when it was opened, therefore the subject of the invention The mite-repelling effect of the mixture has been proven.

The subject of the invention was developed to remove the parasite known as the red mite, chitosan (a linear aminopolysaccharide obtained by deacetylation of chitin) and rosemary Chitin instead of chitosan in the natural mixture containing (Rosmarinus officinalis L.) oil can be used, but chitosan has two great advantages over the kit. Of these first, to dissolve chitin, which can be toxic, such as lithium chloride and dimethylacetamide. Despite the use of solvents, chitosan is readily dissolved in dilute acetic acid. is solvable. Its second advantage is that it is the active part for many chemical reactions. It has free amine groups.

The scope of protection of this application has been determined in the claims section and above It certainly cannot be limited to what is described for illustrative purposes. A technical expert One can also reveal the innovation revealed in the invention by using similar structures. and/or use this configuration with other similar purposes used in the related technique. It is clear that it can be applied to fields as well. Therefore, such structures are especially In the presence of our application, the criterion of innovation and exceeding the known state of the technique It is also obvious that it will be lacking.

REQUESTS . Invention, which leads to deterioration of animal welfare in poultry farming, Developed to remove the parasite known as the winged red mite. 0.5% by weight preferred, 0.1-1% powder chitosan, which can be used by weight, Preferred by weight 2.5% usable by weight 2-3% acetic acid, 0.7% by weight preferred 0.1-1% glycerol, which can be used by weight, 0.5% preferred by weight can be used by weight 0.1-1% tween 80, Preferred by weight, 94.8% by weight usable 80-150% pure water It is a natural mixture containing; 0.5-2% rosemary oil that can be used by weight, 1% by weight, characterized by its content.

Invention, which leads to deterioration of animal welfare in poultry farming, Developed to remove the parasite known as the winged red mite. Preparation of acetic acid solution Adding powder chitosan to acetic acid solution, Adding glycerol to the solution containing acetic acid and chitosan, Centrifugation of the solution Adding tween 80 to the mix Homogenization of the mixture It is a natural mixture produced with process steps and its feature is; mix tween 80 extra In addition to the tween 80 added in the process step, 1% by weight is preferred. 0.5-2% rosemary oil, usable by weight, with the addition of the process step is to be characterized. 3. Removing the parasite known as the winged red mite according to claim-Z Added to the mixture, belonging to the production stage of the natural mixture developed for the purpose of In addition to tween 80, adding rosemary oil is a process step and its feature is; After mixing the mixture for 10 minutes in a magnetic stirrer, add rosemary oil, is added to the solution in drops.

Developed to Fight Red Mite Natural Mixture Containing Chitosan and Rosemary Oil The invention is used in poultry farming, which causes deterioration of animal welfare. the parasite known as the red mite (dermanyssus gallinae) or the red mite for short, Developed to remove chitosan (obtained by deacetylation of chitin) a linear aminopolysaccharide) and rosemary (Rosmarinus officinalis L.) oil. it's about karma.

Claims (3)

REQUESTS 1. Invention, developed to remove the parasite known as winged red mite, which causes deterioration of animal welfare in poultry breeding, 0.5% by weight preferred 0.5% by weight can be used 0.1-1% powder chitosan, 2.5% by weight preferred 2-3% acetic acid, 0.7% by weight preferred °/o.1-1 glycerol, 0.5% by weight preferred 0.5% by weight usable 0.1-1 tween 80, 94.8% by weight preferred It is a natural mixture containing 80-150% pure water that can be used by weight. It is characterized by the fact that it contains 0.5-2% rosemary oil, which can be used by 1%, which is preferred by weight. 2. Invention, preparation of acetic acid solution developed to remove the parasite known as winged red mite, which causes deterioration of animal welfare in poultry breeding, Adding chitosan powder to the acetic acid solution, Adding glycerol to the solution containing acetic acid and chitosan, Precipitating the solution by centrifugation Adding tween 80, homogenizing the mixture. It is a natural mixture produced with process steps. The addition of tween 80 to the mixture is characterized by the addition of 1% by weight usable 0.5-2% rosemary oil, which is preferred by weight, in addition to the tween 80 added in the process step. 3. It is the process step of adding rosemary oil in addition to the tween 80 added to the mixture, which belongs to the production stage of the natural mixture developed to remove the parasite known as the winged red mite according to claim-Z. After mixing the mixture for 10 minutes in a magnetic stirrer, rosemary oil is added to the solution in drops.