TH11461Y - Amyloid beta test strip and reagent kit. - Google Patents

Abstract

The new request improves the amyloid beta test strip and reagent kit. The main components are the amyloid beta test strip itself, composed of a nitrocellulose membrane, a sample pad, and an absorbance pad. These three components are attached to a backing pack and move via antibodies against amyloid beta at the test area and antibodies against anti-mouse IgG at the control area on the nitrocellulose membrane. A reagent kit is used to supplement the amyloid beta test strip. The amyloid beta test strip and reagent kit can detect amyloid beta at the nanogram to picogram level, allowing for screening of patients at risk of Alzheimer's disease. The presence of amyloid beta can be detected before a full diagnostic process is completed. Amyloid beta test strips and reagent kits are a quick, convenient, cost-effective, small, portable, and easily interpretable method for self-diagnosis by the public without the need for expert assistance.

Claims (10)

New request updated. 1. The amyloid beta test strip is a rectangular sheet consisting of a nitrocellulose membrane (a membrane made of cellulose) with one end connected to a sample pad and the other end connected to an absorbent pad. The sample pad is a sheet made of glass fibers used as the area for dropping the sample for analysis. The absorbent pad is a material that absorbs the remaining liquid from the reaction. The backing pack is a material that connects to the bottom of the nitrocellulose membrane. The sample pad and absorbent pad serve to hold the components of the biosensor together, with the upper area of the nitrocellulose membrane acting as the support. The nitrocellulose membrane is divided into two regions: a test region, which is coated with an anti-amyloid beta antibody, and a control region, which is coated with an anti-amyloid beta antibody. 2. Amyloid beta test strip, according to claim 1, where the appropriate substrate is a PVC sheet. 3. An amyloid beta test strip, according to either claim 1 or 2, where the appropriate antibody against amyloid beta is a polyclonal antibody. 4. Amyloid beta test strip, according to one of the claims 1-3, in which the appropriate concentration of anti-amyloid beta antibody and anti-anti-amyloid beta antibody coated at 0.5 µg/ml. 5. An amyloid beta test strip, according to any one of claims 1-4, which has the appropriate dimensions of 0.5 centimeters wide and 6 centimeters long. 6. Amyloid beta test strip, according to any one of claims 1-5, where the appropriate size of the nitrocellulose strip is 0.5 centimeters wide and 3 centimeters long. 7. Amyloid beta test strip, as per any one of claims 1-6, which may be supplemented with the following amyloid beta test reagents: Reagent A: a solution containing a monoclonal anti-amyloid-beta antibody linked to gold particles. Reagent B: a solution containing an anti-anti-amyloid beta antibody linked to gold particles. Reagent C: a solution to facilitate sample flow on the test strip, consisting of 0.08% polyvinyl alcohol and polyethylene glycol. Polyethylene glycol 0.2%, sucrose 0.02%, TritonX-100 0.2%, and Tween-20 0.2% in a phosphate buffer solution with a pH of 7.2. 8. Amyloid beta test strip, in accordance with claim 7, where the appropriate anti-amyloid beta antibody is anti-mouse IgG. 9. The amyloid beta test strip must be passed according to claim 7 or 8, where the optimal gold particle size for use as a component of the amyloid beta test strip is 30-40 nanometers. 10. Amyloid beta test strip, according to any one of claims 1-9, which has the following method for detecting amyloid beta: a. Mix the sample with reagent A in a 1:1 ratio and leave at 25-30°C for 5 minutes. b. Then mix with reagent B in a 2:1 ratio, shake to homogeneize the solution, and leave at 25-30°C for another 5 minutes. c. Then drop the entire mixture onto the amyloid beta test strip at the sample area and add 100 µL of reagent C. Wait until the experimental result appears.