CN105891460B - Portable multifunctional detection means and its application and method based on paper chip - Google Patents
Portable multifunctional detection means and its application and method based on paper chip Download PDFInfo
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- CN105891460B CN105891460B CN201610202958.8A CN201610202958A CN105891460B CN 105891460 B CN105891460 B CN 105891460B CN 201610202958 A CN201610202958 A CN 201610202958A CN 105891460 B CN105891460 B CN 105891460B
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- 238000001514 detection method Methods 0.000 title claims abstract description 123
- 238000000034 method Methods 0.000 title abstract description 12
- 239000007788 liquid Substances 0.000 claims abstract description 24
- 239000012472 biological sample Substances 0.000 claims abstract description 18
- 238000005213 imbibition Methods 0.000 claims abstract description 13
- 125000006850 spacer group Chemical group 0.000 claims abstract description 13
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 8
- 239000000428 dust Substances 0.000 claims abstract description 7
- 238000012360 testing method Methods 0.000 claims description 22
- 239000000427 antigen Substances 0.000 claims description 15
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 14
- 239000008103 glucose Substances 0.000 claims description 14
- 102000036639 antigens Human genes 0.000 claims description 13
- 108091007433 antigens Proteins 0.000 claims description 13
- LEHOTFFKMJEONL-UHFFFAOYSA-N Uric Acid Chemical compound N1C(=O)NC(=O)C2=C1NC(=O)N2 LEHOTFFKMJEONL-UHFFFAOYSA-N 0.000 claims description 12
- TVWHNULVHGKJHS-UHFFFAOYSA-N Uric acid Natural products N1C(=O)NC(=O)C2NC(=O)NC21 TVWHNULVHGKJHS-UHFFFAOYSA-N 0.000 claims description 12
- 229940116269 uric acid Drugs 0.000 claims description 12
- 239000012188 paraffin wax Substances 0.000 claims description 6
- 238000012546 transfer Methods 0.000 claims description 6
- 102000007066 Prostate-Specific Antigen Human genes 0.000 claims description 5
- 108010072866 Prostate-Specific Antigen Proteins 0.000 claims description 5
- 108010015776 Glucose oxidase Proteins 0.000 claims description 3
- 239000004366 Glucose oxidase Substances 0.000 claims description 3
- 108010001336 Horseradish Peroxidase Proteins 0.000 claims description 3
- 102000003992 Peroxidases Human genes 0.000 claims description 3
- 108010092464 Urate Oxidase Proteins 0.000 claims description 3
- 229940116332 glucose oxidase Drugs 0.000 claims description 3
- 235000019420 glucose oxidase Nutrition 0.000 claims description 3
- XMBWDFGMSWQBCA-UHFFFAOYSA-N hydrogen iodide Chemical compound I XMBWDFGMSWQBCA-UHFFFAOYSA-N 0.000 claims description 3
- 239000000463 material Substances 0.000 claims description 3
- 108040007629 peroxidase activity proteins Proteins 0.000 claims description 3
- 239000000523 sample Substances 0.000 abstract description 14
- 230000002745 absorbent Effects 0.000 abstract description 8
- 239000002250 absorbent Substances 0.000 abstract description 8
- 238000012986 modification Methods 0.000 abstract description 4
- 230000004048 modification Effects 0.000 abstract description 4
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 6
- 239000010902 straw Substances 0.000 description 6
- 238000001179 sorption measurement Methods 0.000 description 5
- 238000002965 ELISA Methods 0.000 description 4
- 206010028980 Neoplasm Diseases 0.000 description 4
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 4
- 238000005406 washing Methods 0.000 description 4
- 206010060862 Prostate cancer Diseases 0.000 description 3
- 208000000236 Prostatic Neoplasms Diseases 0.000 description 3
- 238000003556 assay Methods 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 230000002494 anti-cea effect Effects 0.000 description 2
- 239000011324 bead Substances 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 239000003086 colorant Substances 0.000 description 2
- 229940088598 enzyme Drugs 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- 235000014655 lactic acid Nutrition 0.000 description 2
- 239000004310 lactic acid Substances 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 240000003291 Armoracia rusticana Species 0.000 description 1
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- VGGSQFUCUMXWEO-UHFFFAOYSA-N Ethene Chemical compound C=C VGGSQFUCUMXWEO-UHFFFAOYSA-N 0.000 description 1
- 241000283973 Oryctolagus cuniculus Species 0.000 description 1
- 239000002033 PVDF binder Substances 0.000 description 1
- 239000012491 analyte Substances 0.000 description 1
- 229940098773 bovine serum albumin Drugs 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 235000021393 food security Nutrition 0.000 description 1
- 125000002791 glucosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)[C@H](O1)CO)* 0.000 description 1
- 238000003018 immunoassay Methods 0.000 description 1
- 210000001161 mammalian embryo Anatomy 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- PQIOSYKVBBWRRI-UHFFFAOYSA-N methylphosphonyl difluoride Chemical group CP(F)(F)=O PQIOSYKVBBWRRI-UHFFFAOYSA-N 0.000 description 1
- 150000002978 peroxides Chemical class 0.000 description 1
- 229920002981 polyvinylidene fluoride Polymers 0.000 description 1
- 238000007639 printing Methods 0.000 description 1
- 210000002307 prostate Anatomy 0.000 description 1
- 239000004575 stone Substances 0.000 description 1
- 239000001993 wax Substances 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
The invention discloses the portable multifunctional detection means based on paper chip and its application and method, the portable multifunctional detection means includes transparent liquid absorbent device and is placed in the paper chip at nearly imbibition end in transparent liquid absorbent device；Described paper chip one end is arranged to non-detection area of the detection zone one end for modification lyophobic dust, by imbibition end and including at least three spacer bar banded structure, the spacer bar banded structure is connected and is coated with the capturing agent for detecting biological sample the detection zone with non-detection area；The portable multifunctional detection means can be used in biology sample detection, simple and quick, can save detection time, and required sample to be tested amount is few, provides strong tool and method for PoC detections, can apply the detection fields such as medical treatment, environment and food.
Description
Technical field
The invention belongs to field of biological detection, and in particular to the portable multifunctional detection means based on paper chip, also relate to
And application and the method using portable multifunctional detection means detection biological sample of the portable multifunctional detection means.
Background technology
In recent years, in laboratory medicine and clinical field, it is popular that point of care (PoC) tests turns into a comparison
Vocabulary.It appears in the U.S. earliest, and it means：Diagnosed in the place fallen ill and occur event, monitor and treat, energy
Quickly obtain assay and make correct processing in time.Typically, disease examination is the centralized laboratories in hospital
Carry out, but shortcoming is can not to obtain assay in time.So targetedly treatment cannot be immediately begun to, while this detection
Also limited by place.PoC detection can be described as having filled up the blank of quick detection.At present, PoC is for some projects
Detection realizes qualitative results to quantitative parameter, anywhere can in time be detected and layman can also
Operated.This detection can widely apply to family, school, and among work.And it is also not limited to cure
In terms for the treatment of, the detection fields such as environment, food can also be applied to, be especially applicable to the bad place of some medical conditions and hair
It is national in exhibition.PoC has following advantage：It is quick first, test experience result can be obtained within a short period of time；Next makes
With simple, using not by the time, place, the limitation of time, 24 hours comprehensive for service for patients；It is last cost-effective,
Equally accurately result is obtained in the case that dosage is less.But existing detection method or detection instrument can be realized in time just
Ground detects less, limits PoC application.
Therefore, it is badly in need of developing a kind of detection method that can be used in PoC or detection instrument, quickly to obtain assay simultaneously
Correct processing is made in time, it is significant to detection fields such as medical treatment, environment and food.
The content of the invention
In view of this, an object of the present invention is to provide a kind of portable multifunctional detection dress based on paper chip
Put；The second object of the present invention is to provide the portable multifunctional detection means based on paper chip in detection biological sample
In application；The third object of the present invention is to provide the method using portable multifunctional detection means detection biological sample.
For achieving the above object, the present invention provides following technical scheme：
1st, the portable multifunctional detection means based on paper chip, the portable multifunctional detection means include transparent suction
Liquid device and the paper chip for being placed in nearly imbibition end in transparent liquid absorbent device；Described paper chip one end is arranged to the detection zone other end as modification
The non-detection area of lyophobic dust, the detection zone is by imbibition end and including at least three spacer bar banded structure, the spacer bar
Banded structure be connected with non-detection area and be coated with detection biological sample capturing agent.
In the present invention, the transparent liquid absorbent device is transparent liquid absorbent pipe or transparent liquid transfer gun head；The lyophobic dust is stone
Wax；The parting bead banded structure is preferably 3.
Preferably, the belt structure is preferably triangular in shape.
It is furthermore preferred that the portable multifunctional detection means is to detect the device of glucose and uric acid simultaneously；It is or described
Portable multifunctional detection means is to carry out the detection means of EUSA.
The belt structure for detecting the device of glucose and uric acid is 3, respectively control test passage, glucose detection
Passage and uric acid sense channel, the belt structure tip of the glucose detection passage adds liquor kalii iodide, apart from band
Shape structure tip 0.3-0.8cm region adds glucose oxidase and horseradish peroxidase；The uric acid sense channel
Belt structure tip adds uricase, and TMB and peroxide are added apart from 0.3~0.8cm of belt structure tip region
Enzyme；The control test passage is adding the peroxidase of TMB sums apart from 0.3~0.8cm of belt structure tip.
The structure of the detection means of EUSA is as follows：Parting bead banded structure is 3, respectively control test
Passage, carcinomebryonic antigen sense channel and prostate specific antigen sense channel, the ribbon knot of the carcinomebryonic antigen sense channel
Structure part adds anti-carcinoembryonic antigen monoclonal antibody；The belt structure part of the prostate specific antigen sense channel adds anti-
With prostate specific antigen monoclonal antibody；The control test passage is apart from belt structure part not sessile antibody.
2nd, application of the portable multifunctional detection means based on paper chip in biological sample is detected.
Preferably, the biological sample is glucose and uric acid；Or the biological sample is that carcinomebryonic antigen and prostate are special
Hapten.
3rd, the method for detecting biological sample using the portable multifunctional detection means based on paper chip, will be to be detected
Liquid sucks the detection zone that the portable multifunctional detection means soaks paper chip to liquid to be detected, colour developing.
The beneficial effects of the present invention are：The present invention is combined based on paper chip with aspirator, can be realized and more be accelerated
Prompt detection, its paper chip are paper, wide material sources and price is cheap, and can be selected according to the needs of the experiment of oneself different
The needs of paper of material and function is to meet oneself.
In addition, one or more spacer bar banded structures are set to form different detection paths, Neng Goushi in paper chip
Detected while existing a variety of biological samples.For convenience of test in laboratory, paper chip can be put into liquid transfer gun head, utilize liquid-transfering gun
Imbibition, while imbibition, you can complete detection.Testing sample amount needed for the portable multifunctional detection means of the present invention is few,
Only need 100 μ L to complete to detect, provided conveniently for PoC detections.
The portable multifunctional detection means of the present invention can be coated with catching for detection biological sample in detection zone as needed
Agent is obtained, can be applied to the detection fields such as medical treatment, environment and food, to realizing that PoC detections are significant.
Brief description of the drawings
In order that the purpose of the present invention, technical scheme and beneficial effect are clearer, the present invention provides drawings described below：
Fig. 1 is portable multifunctional structure of the detecting device and detection method schematic diagram.
Fig. 2 is that portable multifunctional detection means detects glucose and uric acid result figure simultaneously.
Embodiment
Below in conjunction with accompanying drawing, the preferred embodiments of the present invention are described in detail.It is unreceipted specific in embodiment
The experimental method of condition, generally according to normal condition or according to the condition proposed by manufacturer.
First, the portable multifunctional detection means based on paper chip
The present invention relates to the portable multifunctional detection means based on paper chip, its structure are as shown in Figure 1.As shown in Figure 1,
Portable multifunctional detection means includes transparent liquid absorbent device and is placed in transparent liquid absorbent device by the paper chip of liquid inlet, wherein refill
Piece is paper, and one end is with hydrophilic detection zone, and the other end is that hydrophobicity prevents the non-of cross pollution between different testing sample
Detection zone, the detection zone include one or more spacer bar banded structures, the multiple spacer bar banded structure and non-detection
Area be connected and be coated with detection biological sample capturing agent；The non-detection area is modified by lyophobic dust, such as paraffin；Then will
Paper chip non-detection area is crimped, then imbibition end of the detection zone of paper chip to aspirator is placed in pipette, is obtained based on refill
The portable multifunctional detection means of piece.
In the present invention, the belt structure is at least 3, including at least one setting check plot, and remaining is arranged to try
Test area；Preferably, belt structure is triangle.
In the present invention, transparent liquid absorbent device can use the conventional liquid-transfering gun pipette tips in laboratory or other aspirators, such as once
Property washing liquid device.
The paper of paper chip is preferably filter paper in the present invention.
2nd, the preparation method of the portable multifunctional detection means based on paper chip
The preparation method of portable multifunctional detection means based on paper chip, comprises the following steps：
(1) paper chip is made：By paper cutting instruments to square shape paper one end to be cut into the belt structure at least three interval
Multi-channel detection area is formed, then forms non-detection area in the terminal modified lyophobic dust of connection of filter paper belt structure, such as：Will
Paraffin is modified by paraffin printing technique；Coating detects the capturing agent of biological sample on the belt structure of filter paper again, then
The non-detection area of filter paper is curled into column, forms paper chip；
(2) assemble：The non-detection area of paper chip obtained by step (1) is positioned over pipette from remote imbibition end to nearly imbibition end
Or in the pipette tips of liquid-transfering gun, obtain the portable multifunctional detection means based on paper chip.
In the present invention, at least one belt structure is arranged to control test passage, and remaining is arranged to testing inspection passage,
Different samples and control can be shown that the biological sample of various concentrations can show different colors by different colors,
Therefore qualitative detection and quantitative detection can be realized simultaneously.By being coated with different capturing agents, portable multifunctional in detection zone
Detection means may be implemented in medical treatment, health, food security, environment measuring etc. and be widely used.
In the present invention, belt structure is preferably triangle, and paper is preferably Waterman filter paper.
3rd, the method that biological sample is detected using the portable multifunctional detection means based on paper chip
Detection method is sucked to be detected as shown in figure 1, as shown in Figure 1, during detection using portable multifunctional detection means
Liquid soaks the detection zone of paper chip to liquid to be detected, you can completes the multi-analyte immunoassay in liquid to be detected.Use the more of the present invention
Function portable detector can realize the detection of various concentrations biological sample, and exclude the nonspecific interference of sample, tool
Have the advantages that result is accurate, measurement is accurate, required testing sample amount is few, and 100 μ L can complete to detect.
Application Example 1：
Portable multifunctional detection means detects glucose and uric acid simultaneously：Portable multifunctional is prepared according to the method described above
The belt structure that detection means, specifically filter paper are cut into 3 intervals forms 3 sense channels, respectively control test passage,
Glucose detection passage and uric acid sense channel, filter paper top modification paraffin is then prevented into different sense channel cross pollutions,
And the liquor kalii iodide that 1 μ L concentration is 0.6M is added in the belt structure tip of glucose detection passage, in room temperature (18~25
DEG C) under dry after, apart from belt structure tip 0.3-0.8cm region add 1 μ L by volume be with 5:1 mixing
Glucose oxidase and horseradish peroxidase, dried under the conditions of room temperature (18~25 DEG C)；In the band of uric acid sense channel
Shape structure tip adds 2 μ L uricase (24UmL-1), adding 1 μ apart from belt structure tip 0.3-0.8cm region
L 2mg mL-1TMB, 0.5 μ L 1mg mL are then added in same position-1Peroxidase；And control test is led to
Road is adding 1 μ L 2mg mL apart from belt structure tip 0.3-0.8cm-1TMB and 0.5 μ L 1mg mL-1Peroxide
Compound enzyme.
Then it is column by the non-detection area curling of paper chip, and is put into 1000 μ L pipette tips.
Need detection when, using liquid-transfering gun suck 100 μ L detected sample (glucose or lactic acid or glucose add lactic acid
Mixed liquor), testing result is as shown in Figure 2.As a result show, become orange in glucose detection channel detection zone；Uric acid is examined
The detection zone for surveying passage then becomes au bleu, and check plot is without color.And each sense channel is with the difference of concentration, the depth of color
Shallow difference.
Therefore, portable multifunctional detection means of the invention can be used in same reaction tube while realize multicomponent
Detect on the spot in time.
Application Example 2：
Portable multifunctional detection means is completed to add heart method EUSA (ELISA) while detects carcinomebryonic antigen
With prostatic cancer specific tumour antigen：Portable multifunctional detection means is prepared according to the method described above, specifically gathers inclined difluoro
The belt structure that ethene (PVDF) film cuts into 3 intervals forms 3 sense channels, respectively control test passage, cancer embryo
Antigen sense channel and prostatic cancer specific tumour antigen sense channel, filter paper top modification paraffin is then prevented into different inspections
Survey passage cross pollution, and carcinomebryonic antigen (CEA) sense channel belt structure part add 5 μ L concentration be 10 micrograms/
The anti-carcinoembryonic-antigen (CEA) antibody (anti-CEA Ab) of milliliter, the adsorption reaction 30 minutes under room temperature (18~25 DEG C)；In prostate cancer
The belt structure part of specific tumor antigen (PSA) sense channel adds 5 μ L anti-psa antibody (10 mcg/ml)；And
Control test passage is apart from belt structure part not sessile antibody.Then it is column by the non-detection area curling of paper chip,
And it is put into 1000 μ L pipette tips.Using opaque straw or pipettor/rifle, 150 μ L bovine serum albumin(BSA)s (1%, BSA) are sucked,
The adsorption reaction 30 minutes under room temperature (18~25 DEG C).When needing detection, 100 μ L detected sample is sucked using liquid-transfering gun
(containing CEA, or PSA or CEA and PSA mixed liquor), the adsorption reaction 30 minutes under room temperature (18~25 DEG C).Then, shifting is utilized
Liquid device or opaque straw are repeatedly inhaled, blow and (sample introduction, go out sample) action, complete the washing in ELISA steps.Utilize opaque straw or shifting
Liquid device/rifle, suck the 100 anti-CEA in μ L sources containing mouse, the solution (0.1 mcg/ml) of the monoclonal antibody of anti-psa, in room temperature (18
~25 DEG C) under adsorption reaction 30 minutes；Then, repeatedly inhaled using pipettor or opaque straw, blow and (sample introduction, go out sample) action, it is complete
Washing into ELISA steps.Hereafter, using opaque straw or pipettor/rifle, 100 μ L horseradish peroxidase-labeleds are sucked
Rabbit anti-mouse antibody, the adsorption reaction 15 minutes under room temperature (18~25 DEG C).Then, it is multiple using pipettor or opaque straw
Inhale, blow and (sample introduction, go out sample) action, complete the washing in ELISA steps.Finally, 100 μ L TMB solution is sucked using liquid-transfering gun.
As a result show, become au bleu in CEA sense channels detection zone；Check plot is without color.And each sense channel is with concentration
Difference, the depth of color are different.
Finally illustrate, preferred embodiment above is merely illustrative of the technical solution of the present invention and unrestricted, although logical
Cross above preferred embodiment the present invention is described in detail, it is to be understood by those skilled in the art that can be
Various changes are made to it in form and in details, without departing from claims of the present invention limited range.
Claims (3)
1. the portable multifunctional detection means based on paper chip, it is characterised in that：The portable multifunctional detection means bag
Include transparent liquid transfer gun head and be placed in the paper chip at nearly imbibition end in transparent liquid transfer gun head；Described paper chip one end is arranged to detect
Area, for the other end to modify the non-detection area of lyophobic dust, the detection zone includes 3 spacer bar banded structures by imbibition end,
The spacer bar banded structure be connected with non-detection area and be coated with detection biological sample capturing agent；
3 spacer bar banded structures are triangle, respectively control test passage, glucose detection passage and uric acid detection
Passage, the belt structure tip of the glucose detection passage adds liquor kalii iodide, apart from belt structure tip 0.3-
0.8cm region adds glucose oxidase and horseradish peroxidase；The belt structure tip of the uric acid sense channel
Uricase is added, TMB and peroxidase are added apart from the cm of belt structure tip 0.3 ~ 0.8 region；The control test
Passage is adding TMB and peroxidase apart from the cm of belt structure tip 0.3 ~ 0.8 region.
2. the portable multifunctional detection means based on paper chip according to claim 1, it is characterised in that：The hydrophobicity
Material is paraffin.
3. the portable multifunctional detection means based on paper chip, it is characterised in that：The portable multifunctional detection means bag
Include transparent liquid transfer gun head and be placed in the paper chip at nearly imbibition end in transparent liquid transfer gun head；Described paper chip one end is arranged to detect
Area, for the other end to modify the non-detection area of lyophobic dust, the detection zone includes 3 spacer bar banded structures by imbibition end,
The spacer bar banded structure be connected with non-detection area and be coated with detection biological sample capturing agent；
3 spacer bar banded structures are triangle, respectively control test passage, carcinomebryonic antigen sense channel and prostate
Specific antigen sense channel, the belt structure part of the carcinomebryonic antigen sense channel add anti-carcinoembryonic antigen monoclonal antibody；
The belt structure part of the prostate specific antigen sense channel adds anti-prostate-specific-antigen monoclonal antibody；It is described
The belt structure part of control test passage not sessile antibody.
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| CN201610202958.8A CN105891460B (en) | 2016-03-31 | 2016-03-31 | Portable multifunctional detection means and its application and method based on paper chip |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106442491A (en) * | 2016-09-07 | 2017-02-22 | 华东理工大学 | Method for rapidly detecting urine sugar and uric acid in solution |
| CN110045124B (en) * | 2019-04-01 | 2024-07-19 | 芜湖森爱驰生物科技有限公司 | Interleukin-6 detection kit |
| CN111879934A (en) * | 2020-08-12 | 2020-11-03 | 上海市动物疫病预防控制中心(上海市兽药饲料检测所、上海市畜牧技术推广中心) | Canine carcino-embryonic antigen immune comb and canine carcino-embryonic antigen detection kit |
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| CN204439557U (en) * | 2014-09-26 | 2015-07-01 | 北京京东世纪贸易有限公司 | Tubular type test paper |
| CN204556510U (en) * | 2015-04-10 | 2015-08-12 | 山东省农业科学院畜牧兽医研究所 | A kind of sealed type ph test paper |
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| CA2564310A1 (en) * | 2005-11-07 | 2007-05-07 | Nisshinbo Industries, Inc. | Tip for biomolecular reaction |
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| US5876918A (en) * | 1993-03-08 | 1999-03-02 | Hydros, Inc. | Aligned fiber diagnostic chromatography with positive and negative controls |
| CN1456892A (en) * | 2003-06-06 | 2003-11-19 | 浙江大学 | Whole blood uric acid concentration detecting paper strip |
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| CN204439557U (en) * | 2014-09-26 | 2015-07-01 | 北京京东世纪贸易有限公司 | Tubular type test paper |
| CN104697993A (en) * | 2015-02-10 | 2015-06-10 | 吴有财 | Urine data acquiring device and application method thereof |
| CN204556510U (en) * | 2015-04-10 | 2015-08-12 | 山东省农业科学院畜牧兽医研究所 | A kind of sealed type ph test paper |
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