SU818603A1 - Method of diagnosis of alveococcus - Google Patents

Description

one

This invention relates to the field of medicine, in particular to methods for diagnosing helminthism, in particular alveococcosis.

A known method for the diagnosis of alveococcosis by examining blood serum by setting serological and immunological reactions 1.

However, it is necessary to set up at least two or three different immunological reactions using, as a rule, deficient diagnostics. The method allows patients to be diagnosed only in the second (the manifestation of clinical signs of disease) and the third (the presence of complications) stages, i.e., in later cases, when the treatment may not have the desired effect.

The aim of the invention is the early diagnosis of the disease.

The goal is achieved by determining the activity of phosphohexoisomerase, aldolase, and ketozo-1-phosphataldolase in the urine, and at a ratio of

aldolase

Ketozo-phosphate aldolase 0.56-0.87, and

ketozo-1-phosphataldolase phosphohexoisomerase ratio

equal 0,47-0,66, diagnose early stage of alveococcosis.

The activity of urine enzymes is investigated by methods known for determining these values in serum: the activity of aldolase by the method of V.A. Ananieva and V.R. Obukhova, ketozo-1-phosphataldolase by the method of DM Braginsky et al., Phosphohexoisomerase by the method

B. F. Korovkina, and double the amount of substrate is used to analyze the activity of aldolase and ketozo-1-phosphataldolase.

The intensity of the formed colors in the test and control samples was determined by colorimetric using a cuvette with 5 mm working faces for distilled water with a 536 nm light filter, ketos-1 phosphate aldolase — a cell with 5 mm working faces against distilled water with a 5 mm working face, 5 phosphohexoisomerases using a cuvette with 10 mm working faces against controls for reagents with a 413 nm light filter. The readings of the galvanometer are translated into extinction rates. The activity of urine enzymes is expressed in units of extinction, multiplied by 100 (EXO). Ratios that reflect the relationship between enzyme activity are calculated.

urine.

In individuals in the early (lethal) stage of the disease with alveococcosis, enzymatic koefaldosis

fcient rises

Ketozo-1-phosphotaldolase

From 0.25 (in healthy people) to

Urine Enzyme Activity (Extraction Units)

ketozo-1-phosphataldolase

0.58-0.87, coefficient

phosphohexoisomerase is reduced from 2.20 (in healthy people) to 0.47–0.66.

The results of the studies are presented in the table.

where M is arithmetic average;

/ and - arithmetic average error; p - probability.

As follows from the data presented in the table, the proposed method allows for the early diagnosis of alveococcosis and, as a result, the timely treatment of the disease.

Claims (1)

1. Zorikhina V. I, “Serological methods for the diagnosis of human echinococcosis and human alveococcosis and their use in studying the epidemiology of these diseases. “Abstract of thesis for the degree of Doctor of Medical Sciences. M., 1978.