SU1754071A1 - Method for making differential diagnosis of chronic hepatitis and cholecystocholangitis - Google Patents

Abstract

Usage: the invention relates to laboratory tests and can be used to diagnose diseases of the hepatobiliary system. The goal is to improve the accuracy of the method. The essence of the method lies in the fact that on an empty stomach saliva after 2 times crawling of the mouth of the distilled gray-haired distillate is collected, centrifuged, the supernatant in the amount of 0.02 ml is applied to a glass slide (sample) and closed with a cover slip. The preparation of saliva in the genetic chamber thus formed is left for 24 hours at room temperature. The intensity of light transmission of the formed birefringent structures is measured on a spectrophotometer. Results are expressed in arbitrary optical units. When the density of liquid crystals of saliva 15 - 30 units. chronic hepatitis is diagnosed, and at a density of 35 units and above, chronic cholecystocholangitis is diagnosed with S. The positive effect of the invention is the C / 1 increase in the accuracy of differential f diagnostics of chronic hepatitis and cholestasis of cystocholangitis. 1 tab.

Description

The invention relates to medicine, in particular to laboratory studies of saliva in diseases of the hepatobiliary system.

Biochemical methods for the study of saliva (protein, glycoproteins, lysozyme, etc.) are known, which are used in assessing the functional state of the digestive organs.

However, these studies are complex in their performance and characterize only certain aspects of the metabolic processes, changing in different stages of the disease.

Closest to the proposed method is the study of solid-crystal structures in various biological substrates: duodenal content, tears, blood serum.

The disadvantages of this method are the lack of a quantitative assessment and the difficulty in interpreting the crystallographic pattern.

The aim of the invention is to improve the accuracy of the method.

This goal is achieved by using saliva as a biological fluid under test, 0.02 ml of which was placed on a glass slide and covered with a cover glass (both glasses were degreased beforehand). The unpressurized chamber created in this way was kept for 24 hours with a combo.

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This temperature was then placed in a spectrophotometer, on which the intensity of the light transmission of the birefringent structures formed was measured.

The results of the follow-up are presented in tables.

These tables indicate the informativeness of this test.

Example 1. Ill M., 13 years old, was in DSO MONIKI with a diagnosis of Chronic cholecystocholangitis in the acute stage. The diagnosis was made on the basis of anamnesis, clinical, laboratory and X-ray examination.

The intensity of transmisson through an unpressurized cell with a saliva sample was 67 srvc. optical units

Comparing the results of crystallographic drawing of saliva in this patient and in other children with a similar picture of the disease, it can be concluded that this method helps to establish the correct diagnosis in young children who have difficulty in endoscopic and probe studies. In addition, it can be used in mass screening of children for detecting health group II and lit.

The proposed method provides a reduction in the timing of the examination of the patient, avoids carrying out various guest rooms for the patient's procedures associated with the use of anesthesia (liver biopsy puncture) or causing moral injury to the child (probe studies). It may be a prerequisite for choosing adequate treatment and stopping the dispensary registration of children at risk for developing diseases of the hepatitis B system, especially after transferred viral hepatitis.

Example 2, Patient D., 10 years old, was admitted to the clinic with a guide diagnosis: Stomach ulcer.

When conducting saliva crystallography, an optical density of 112 sr. wholesale units. Based on this, a presumptive diagnosis of chronic cholecystocholangitis in the acute stage was made. Further studies performed (fibroesophagogastroscopy, duodenal intubation, echography

abdominal organs) allowed to confirm this diagnosis.

Example 3. Patient Y, 11 years old, was admitted to the clinic with a guide diagnosis: Chronic cholecystocholangitis,

A carried out crystallographic saliva revealed an optical density of 19 uyl, opt. units This study suggested that the patient had chronic hepatitis. Further laboratory and instrumental studies (including liver biopsy) confirmed the diagnosis of chronic active hepatitis.

Example 4. Ill, N., 8 years old, was admitted to the clinic with a guide diagnosis of biliary dyskinesia.

A crystallography of saliva revealed an optical density of 30 uel, opt.

units. As a result of this study, differential diagnosis was made between chronic cholecystocholanitis and chronic hepatitis. With the help of liver biopsy, a diagnosis of chronic persistent hepatitis was made.

The proposed method for the diagnosis of diseases of the hepatobiliary system is quite informative, allows

to quantify more accurately the nature of the pathological process is available for implementation in health care practice.

This diagnostic method allows

to speed up the results of examinations of patients in the hospital and can be applied during mass examinations of the population to identify risk groups for diseases of the hepatobiliary system.

Claims (1)

Invention Formula The method of differential diagnosis of chronic hepatitis and cholecystocholangitis by crystallographic examination of a biological substrate, differing in that, in order to improve the accuracy of the method, crystallography is subjected to saliva in an unpressurized chamber at room temperature for 1 day, then the optical the density of saliva liquid crystals, with a density of 15 - 30 units. diagnosed with chronic hepatitis, and when its density from 35 units. and above, chronic cholecystocholangitis is diagnosed.