SU1355931A1 - Method of determining individual sensitivity to rehydration therapy in case of patients having intestinal infection - Google Patents

Abstract

This invention relates to biochemistry. The purpose of the invention is the early detection of an individual sensitive tee. Two portions of urine, obtained before and after treatment, are treated with a solution of trichloroacetic acid and centrifuged. The supernatant is diluted, the level of optical density is measured, and the adequacy of the administered therapy is determined by the ratio of the extinction values before and after treatment. The method allows to control the effectiveness of rehydration. i (L

Description

The invention relates to medicine, specifically to infectious diseases, and can be used to control the effectiveness of rehydration therapy in patients with acute intestinal infections.

The purpose of the invention is to improve the accuracy of the method.

The method is carried out as follows.

Two portions of urine, obtained immediately before and after rehydration therapy, are abatted with 10% trichloroacetic acid in a ratio of 1: 0.5. Deposition is carried out in a laboratory centrifuge at 3000 rpm. The supernatant containing the acid-soluble fraction of the metabolites is diluted with 1: 100 distilled water, the optical density is determined at 254 nm versus distilled water, the ratio of the indicator in the urine portion obtained after rehydration therapy is calculated to the initial one and at a value greater than 1 is determined adequate efficacy of therapy.

Example 1. B-on 3, diagnosis; food toxicoinfection, medium course, dehydration of II degree. On admission, the patients were diagnosed with adina, nausea, vomiting 5-7 times, abdominal pain, loose stools up to 10 times, body temperature 38.8 ° С. Rehydration was carried out orally. Laboratory indicators; The urine pH is 4.75, the relative density is 1.024 g / cm, the urea content is 220 mmol / l, the content of acid-soluble metabolites (CRM) is 0 „320 units, after the rehydration has been completed, which amounted to 3.5 l of gluco-solan solution, urine analysis, the studied parameters slightly changed compared with baseline, except for the CRM ,. which increased to 0.605 usd. The ratio of the KRM fraction after rehydration to the initial value was (K) 1.8. At the same time, a positive clinical dynamics was observed - nausea, vomiting disappeared, body temperature was normalized. The K-index used and the regression of the clinical symptoms of the disease made it possible to consider rehydration therapy effective.

Example 2. Bs. B., diagnosis: food toxicoinfection, medium course, dehydration of II-III degree. On admission, the patient is pale, cyanosis of the lips, cramps of the calf muscles, vomiting 10 times, loose stools up to 12 times, oliguri. Laboratory data obtained prior to the onset of rehydration: pH 5.2 relative density 1.020 g / cm, urea content 210 mmol / l, CRM 0.280 sr. Rehydration was carried out by injecting intravenously 5 solution of Quartasol, after 3 hours of infusion in the urine analysis, the studied parameters differed little from the initial ones, including the content of the CRM, which amounted to 0.278 standard units, 0 ratio of fraction

was less than 1 (, 98). The persistent signs of intoxication and diarrhea in combination with an unchanged level of CRP in the urine in response to rehydration 5 served as a basis to consider the therapy to be insufficiently effective. After an additional course of oral rehydration with a solution of glucosolan, clinical improvement of co-Q and 100 patients was observed, and consequently, a positive dynamics of CRP excretion was observed. which was expressed by increasing K to 15.3.

Claims (1)

The data obtained using the spectrophotometric study of 35 urine in patients with acute intestinal infections in the process of rehydration therapy shows TOM that the proposed method allows you to monitor the effectiveness of rehydration 5 creates the ability to prevent the occurrence of complications, the development of a protracted course of the disease. Invention Formula The method for determining individual sensitivity to rehydration therapy in patients with intestinal infections by examining urine, characterized in that, for the purpose of early detection of individual sensitivity, urine is examined before and after the minimum treatment time, while the sample is processed 10 % solution of TCA in the ratio of 1: 0.5 d. The supernatant was diluted with 55 distilled water of 1: 100, spectrometrized at 254 nm, and by the ratio of extinction values before and after therapy to the original, more than 1 determine individual sensitivity. nost.