SU806760A1 - Method of producing cultural filtrate of basidial russula decolorans fungus - Google Patents
Method of producing cultural filtrate of basidial russula decolorans fungus Download PDFInfo
- Publication number
- SU806760A1 SU806760A1 SU782686297A SU2686297A SU806760A1 SU 806760 A1 SU806760 A1 SU 806760A1 SU 782686297 A SU782686297 A SU 782686297A SU 2686297 A SU2686297 A SU 2686297A SU 806760 A1 SU806760 A1 SU 806760A1
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- SU
- USSR - Soviet Union
- Prior art keywords
- russula
- decolorans
- fungus
- basidial
- filtrate
- Prior art date
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Description
1one
Изобретение относитс к ферментной технологии и может найти применение в сыроделии дл получени сыров.The invention relates to enzyme technology and may find use in cheese making for the production of cheese.
Известен способ получени культурального фильтрата базидиального гриба Russula decolorans, из которого выдел ют молокосвертывающий фермент, служащий основой молокосвертывающего препарата Руссулин.A known method of obtaining the culture filtrate of the basidiomycete fungus Russula decolorans, from which the milk-clotting enzyme is used, which serves as the basis of the milk-clotting preparation Roussulin.
заключаетс в культивировании продуцента Russula decolorans, выделенного из плодового тела Russula decolorans Fr (сыроежка сереюща ) на жидкой питательной среде с последующим отделением культурального фильтра та от мицели l. It consists in cultivating the producer of Russula decolorans, isolated from the fruit body of Russula decolorans Fr (russula graying) on a liquid nutrient medium, followed by separation of the culture filter and the mycelium l.
Однако получаеьфлй культуральный Фильтрат обладает сравнительно невысокрй молбкосвертывающей активностью Г976 ед/мл)/что в дальнейшем лимитирует активность получаемого ЛерментН .ОГО поепарата (500 000 ед)..However, the resulting cultured filtrate has a relatively low G976 U / ml molar-clotting activity / which further limits the activity of the obtained LermentN. OH of the preparation (500,000 units) ..
Цель изобретени - повышение активности культурального фильтрата базидиального грибка Russula decolorans Поставленна цеЛь достигаетс , что культуральный фильтрат дополнительно пропускают в течение 2-15 ч при 18-20°С через слой карбоксилсОдержащего поливинилспиртового волокна с привитой полиакриловой кислотой .The purpose of the invention is to increase the activity of the culture filtrate of the basidial fungus of Russula decolorans. The goal is that the culture filtrate is additionally passed for 2-15 hours at 18-20 ° C through a layer of carboxyl-containing polyvinyl alcohol fiber with grafted polyacrylic acid.
В результате сорбции ингибиторов небелкового характера волокном молокосвертывающа активность культурального фи-льтрата увеличиваетс в 1,32 ,0 раза.As a result of the sorption of inhibitors of non-protein nature by the fiber, the milk-clotting activity of the culture fiber increases by 1.32, 0 times.
Полученный описываемым способом культуральный фильтрат базидиального гриба Russula decolorans используют как основу дл получени сухого молокосвертывающего ферментного препарата , используемого в сыроделии.The culture filtrate of the basidiomycete fungus Russula decolorans obtained by the described method is used as the basis for the preparation of the dry milk-clotting enzyme preparation used in cheese making.
Препарат, полученный на основе культургшьного фильтрата по предлагаемому способу, обладает молокосвертывающей активностью 800000-850000 ед что в 1,7 раза выше активности препарата на основе культурального лильтрата , получаемого известным способомThe preparation obtained on the basis of the culture filtrate of the proposed method has a milk-clotting activity of 800,000-850000 units, which is 1.7 times higher than the activity of the preparation based on the culture liltrath obtained in a known manner
Пример 1. Посевную культуру выращивают на сусле-агаре при 25-26 С Культивирование продуцента ведут поверхностным способом в колбах Эрленмейера и в биологических матрасс1х, содержащих соответственно 100, 200 и 150 мл питательной среды состава, г/л: глюкоза 10,, пептон 3, КН,РО.О,6 0,4, Мд504-7Н.О О,ОЬ, 70504Example 1. Sowing culture is grown on wort-agar at 25-26 C. Producer cultivation is carried out in a superficial manner in Erlenmeyer flasks and in biological matrices containing, respectively, 100, 200 and 150 ml of the nutrient medium of the composition, g / l: glucose 10, peptone 3 , KN, RO.O., 6 0.4, MD504-7N.O, OH, 70504
Claims (1)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU782686297A SU806760A1 (en) | 1978-11-04 | 1978-11-04 | Method of producing cultural filtrate of basidial russula decolorans fungus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU782686297A SU806760A1 (en) | 1978-11-04 | 1978-11-04 | Method of producing cultural filtrate of basidial russula decolorans fungus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SU806760A1 true SU806760A1 (en) | 1981-02-23 |
Family
ID=20794297
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SU782686297A SU806760A1 (en) | 1978-11-04 | 1978-11-04 | Method of producing cultural filtrate of basidial russula decolorans fungus |
Country Status (1)
| Country | Link |
|---|---|
| SU (1) | SU806760A1 (en) |
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1978
- 1978-11-04 SU SU782686297A patent/SU806760A1/en active
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