SU689313A1 - Method of control of culturing properties of nourishing media for cholera diagnosis - Google Patents
Method of control of culturing properties of nourishing media for cholera diagnosis Download PDFInfo
- Publication number
- SU689313A1 SU689313A1 SU782649889A SU2649889A SU689313A1 SU 689313 A1 SU689313 A1 SU 689313A1 SU 782649889 A SU782649889 A SU 782649889A SU 2649889 A SU2649889 A SU 2649889A SU 689313 A1 SU689313 A1 SU 689313A1
- Authority
- SU
- USSR - Soviet Union
- Prior art keywords
- control
- cholera diagnosis
- media
- agar
- nourishing
- Prior art date
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Description
(54) СПОСОБ КОНТРОЛЕ РОСТОВЫХ СВОЙСТВ ПИТАТЕЛЬНЫХ СРЕД ДЛЯ ДИАГНОСТИКИ ХОЛЕРЫ(54) METHOD FOR CONTROL OF GROWTH PROPERTIES OF NUTRIENT FOR DIAGNOSTIC CHOLERA
Способ иллюстрируетс следующими примерами.The method is illustrated by the following examples.
Пример 1. Дл испытани качества питательных сред используют 5-часовые агаровые культуры тест (дакробов Vibrio Nag Р-9741 и V.Cholerae Р-1/145 18-20 часовых агаровых культур 2-5 пас.сажа.Готов т взвеси вибрионов в физиологическом растворе , содержащие по 2 клеток в 1 мл. Приготовленные взйеси развод т физиологическим раствором в 2 раза/ а затем делают р д последовательных дес тикратных разведений с таким расчетом, чтобы в 1.мл содержгшось 1000 м.т. Из приготовленной взвеси производ т высев по ОД мл на 3 чашки с испытуемой агаровой средой. Дл контрол посевной дозы параллельно делают высев на 3 агаровые пластинки заранее проверенного качественного щелочного агара (рН 7,6-7,8). Посевной материал равномерно распредел ют по поверхности агаровой пластинки путем покачивани чгиики Петри. Посевы выращивгиот при 37°С в течение 12 ч.Example 1. For testing the quality of nutrient media, 5-hour agar cultures were tested (Vibrio Nag P-9741 and V. Pholerae P-1/145 dacrobes 18-20 hour agar cultures of 2-5 pas. Sazha. Ready t suspension of vibrios in physiological solution containing 2 cells in 1 ml. Prepared vodis are diluted 2 times with saline and then made a series of consecutive tenfold dilutions so that 1.ml contained 1000 mt. OD ml per 3 cups with the test agar medium. Doses in parallel make seeding on 3 agar plates of previously tested high-quality alkaline agar (pH 7.6-7.8). The seed is evenly distributed over the surface of the agar plate by rocking Chgiika Petri. Seeds grown at 37 ° C for 12 hours.
Плотные питательные среды считают годными в том случае., если на пластинках агара через 12 ч вырастает не менее 30% колоний от общего посевного числа в типичной форме размером не менее 1 мм.Dense nutrient media are considered suitable in the event that, on agar plates, after 12 hours at least 30% of the colonies of the total seed number in a typical form grows with a size of at least 1 mm.
Пример 2. Дл испытани . качества жидких питательных сред из рабочих взвесей тест-штаммов, приготовленных способом, описанным в примере 1, производ т высевы в объеме 0,1 мл (100 м.т.) в 3 флакона соExample 2. For testing. the quality of liquid nutrient media from working suspensions of test strains prepared by the method described in Example 1, are sown in a volume of 0.1 ml (100 mt) in 3 bottles with
100 мл испытуемой 1% пептонной водой и 3 флакона с заранее проверенной высококачественной 1% пептонной водой. Дл контрол посевной дозы необходимо сделать высев посевной культуры на « три агаровые пластинки. Посевы инкубируют .при в течение 6 ч, после чего из каждого флакона с поверхности высевают петлей на чашку Петри с проверенным высококачественным агаром. Выращивают при З7с 12-18 ч.100 ml of tested 1% peptone water and 3 bottles with pre-tested high-quality 1% peptone water. To control the inoculation dose, it is necessary to make the inoculation of the inoculum on three agar plates. Crops are incubated for 6 hours, after which from each bottle from the surface they are seeded with a loop on a Petri dish with a proven high-quality agar. Grown when S7s 12-18 h.
Пептонную воду считают пригодной в том случае, если в результате 6-часового выраццивани в ней и последующего высева петлей на агаровых пластинках вырастает в среднем не S менее 10 колоний вибрионов.Peptone water is considered suitable if, as a result of 6-hour expression in it and subsequent seeding by a loop on agar plates, an average S does not grow less than 10 vibrio colonies.
Использование изобретени позволит сократить сроки контрол качества питательных сред дл диагностики холеры. 0The use of the invention will reduce the time needed to control the quality of nutrient media for the diagnosis of cholera. 0
Claims (1)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU782649889A SU689313A1 (en) | 1978-07-27 | 1978-07-27 | Method of control of culturing properties of nourishing media for cholera diagnosis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU782649889A SU689313A1 (en) | 1978-07-27 | 1978-07-27 | Method of control of culturing properties of nourishing media for cholera diagnosis |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SU689313A1 true SU689313A1 (en) | 1980-11-30 |
Family
ID=20779343
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SU782649889A SU689313A1 (en) | 1978-07-27 | 1978-07-27 | Method of control of culturing properties of nourishing media for cholera diagnosis |
Country Status (1)
| Country | Link |
|---|---|
| SU (1) | SU689313A1 (en) |
-
1978
- 1978-07-27 SU SU782649889A patent/SU689313A1/en active
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