SU1617337A1 - Method of determining hydroquinone - Google Patents

Abstract

This invention relates to analytical chemistry, in particular to the determination of hydroquinone. The goal is to increase the detection sensitivity. The preparation is carried out by treating the analyzed sample with 4-diethylaminophthalhydrazide in the presence of a solution of cobalt salt (2+) and an alkaline solution of hydrogen peroxide. The method provides for the determination of hydroquinone at its content in the solution (1-100) ^. 10 ^-4 µg / ml, which is 30,000 times the sensitivity of the known method of determination. 1 tab.

Description

The invention relates to analytical chemistry, in particular to methods for the determination of hydroquinone with its content in the solution (1-100) .10 µg / ml, and can be used in biochemical studies, as well as in the analysis of environmental objects, natural and waste paint and varnish leather and textile industry.

The purpose of the invention is to increase the detection sensitivity.

Example 1. In the first cuvette inject 0.5 ml of an 8 M solution of KOH and 0.5 ml of a solution of hydrogen peroxide, and in the second cuvette, a .0.5 ml of an 8-10 M solution of 4-diethyl amino phthalhydride i.O. 1 ml of 10 M solution of cobalt (II) salt and hydroquinone in the amount of O-140 µg / ml. The contents of the second cuvette are introduced into the first one and the maximum intensity of the light is measured after 5 s using a photomultiplier. The content of hydroquinone is found on the calibration curve. The detection sensitivity is 1-10 µg / ml. The graduated graph is linear in the coordinates of IgJ - pC in the concentration range of hydroquinone G-10 - 110 µg / ml.

Example 2. All components were introduced into the first and second cuvettes in the amounts indicated in Example 1, with the exception of hydroquinone, which was introduced into the second cuvette in a volume of 4.0 µg / ml. Analysis I is carried out as described.

in example 1. Hydroquinone h (3, 7 ± 0.6) -PG μg / ml was found (n 6, P 0.95).

Example 3. All components were introduced into the first and second cuvettes in the amounts indicated in Example 1, with the exception of hydroquinone, which was

|

oo with

d | t in the second cuvette in the amount of 8. | 8 × mcg / ml. The analysis is carried out analogously to that described in Example 1. Hydroquinone (8.7lO, 6) -1O µg / ml (TI 6, P 0.95) was found.

| Example 4. In the first and second KidseTM, all components are entered in the numbers given in Example 2 for | with the exception of hydroquinone, which enters into the second cuvette in the amount of 17, 6.-10 μg / ml. The analysis is carried out analogously to Example 1. A hydroquinosome was found for the analysis of natural; with and sewage with a content of hydroquinone n level and below the MPC (0.2 mg / ml).

ten

on

(1b, 6 ± 1,2) .1-0 μg / n1 (n 6, P 0.95),

Example 5. In the first and second cells, all components are entered in quantities.

on

MO

15

TWAs listed in Example 2 for

20

with the exception of hydroquinone, which was supplied in the second cuvette in the amount of 88 × O μg / ml. The analysis is carried out analogously to example 1. Hydroquino-Formulas for the invention were found.

(78 ± 11) -10 μg / ml (q 6,

R

0.95).

The table presents the results of an ogg of hydroquinone in solution (n; measures 2–5). From the table it is clear that

The advanced method is characterized by sufficient accuracy and reproducibility.

; tyu.

Thus, the sensitivity of the op: the hydroquinone effect of the proposed method is 30,000 times greater than the sensitivity of the known method, allows the proposed

thirty

wh

35

The method of determining hydroquinone by treating an analyzed sample with an organic reagent in the presence of a catalyst and an alkaline solution of hydrogen peroxide: followed by measuring the intensity of chemiluminescence, which is used to increase the sensitivity of the determination as 4-diethyl amino acid alhydrazide as an organic reagent and as a catalyst, a solution of cobalt (II) salt.

natural analysis method; with and wastewater when the content of hydroquinone at the level and below the MPC (0.2 µg / ml).

The method of determining hydroquinone by treating an analyzed sample with an organic reagent in the presence of a catalyst and an alkaline solution of hydrogen peroxide: followed by measuring the intensity of chemiluminescence, which is used to increase the sensitivity of the determination as 4-diethyl amino acid alhydrazide as an organic reagent and as a catalyst, a solution of cobalt (II) salt.

Claims (1)

The claims of the definition of hydroquinone in (examples 2-5) pr To the face. Thus, the sensitivity of the determination of hydroquinone according to the proposed method is 30,000 times higher A method for determining hydroquinone by treating an analyzed sample with an organic reagent in the presence of. The table shows the present method is characterized by accuracy and reproduction ι the sensitivity of the known method, which allows the use of the proposed catalyst and an alkaline solution of hydrogen peroxide with subsequent measurement of the chemiluminescence intensity, characterized in that, in order to increase the sensitivity of determination, 4-diethylamino-phthalhydrazide is used as an organic reagent and as a catalyst, a cobalt (II) salt solution.