KR920704117A - Fibrinogen Direct Analysis Method - Google Patents

Fibrinogen Direct Analysis Method

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Publication number
KR920704117A
KR920704117A KR1019920701275A KR920701275A KR920704117A KR 920704117 A KR920704117 A KR 920704117A KR 1019920701275 A KR1019920701275 A KR 1019920701275A KR 920701275 A KR920701275 A KR 920701275A KR 920704117 A KR920704117 A KR 920704117A
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KR
South Korea
Prior art keywords
plasma
sample
concentration
fibrinogen
measuring
Prior art date
Application number
KR1019920701275A
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Korean (ko)
Inventor
줄리에 프란세스 호프만
자네트 블랙 칼라한
찰스 헤르마스 스워프
Original Assignee
에프.지.엠.헤르만스 에이.지.제이.베르미렌
악조 엔. 브이.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by 에프.지.엠.헤르만스 에이.지.제이.베르미렌, 악조 엔. 브이. filed Critical 에프.지.엠.헤르만스 에이.지.제이.베르미렌
Publication of KR920704117A publication Critical patent/KR920704117A/en

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/84—Systems specially adapted for particular applications
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/86—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood coagulating time or factors, or their receptors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/745—Assays involving non-enzymic blood coagulation factors
    • G01N2333/75—Fibrin; Fibrinogen

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Plasma & Fusion (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Steroid Compounds (AREA)

Abstract

내용 없음.No content.

Description

피브리노겐 직접 문석 방법Fibrinogen direct serpentine method

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

Claims (5)

혈액 플라즈마 샘플의 피브리노겐 농도를 광학적으로 측정하는 방법에 있어서, 피브리노겐을 함유하고 있는 플라즈마 샘플을 컨테이너에 제공하는 단계와; 샘플에 트롬빈을 부가하는 단계와; 샘플과 트름빈을 혼합하여 반응 혼합물을 형성하는 단계와; 반응 혼합물에 대한 초기 광학 투과을을 측정하는 단계와; 트롬빈과 피브리노겐을 반응 혼합물내에서 서로 반응시키는 단계와; 반응 혼합물에 대한 최종 광학 투과율을 측정하는 단계와; 델타값을 계산하기 위해서 최종 투과율 측정값과 초기 투파울 측정값을 비교하는 단계와; 델타값에 따라서 피브리노겐 농도를 결정하는 단계를 포함하는 것을 특징으로 하는 측정방법.CLAIMS 1. A method for optically measuring fibrinogen concentration in a blood plasma sample, comprising: providing a container with a plasma sample containing fibrinogen; Adding thrombin to the sample; Mixing the sample with the trumbin to form a reaction mixture; Measuring an initial optical transmission for the reaction mixture; Reacting thrombin and fibrinogen with each other in a reaction mixture; Measuring a final optical transmittance for the reaction mixture; Comparing the final transmittance measurement with the initial two foul measurement to calculate a delta value; Determining the fibrinogen concentration according to the delta value. 제1항에 있어서, 트롬빈은 약 100 NIH 유닛의 농도를 갖고 플라즈마 샘플은 나트륨 바아비탈과 약 1:100의 비율로 희석되는 것을 특징으로 하는 측정방법.The method of claim 1, wherein the thrombin has a concentration of about 100 NIH units and the plasma sample is diluted at a ratio of about 1: 100 with sodium barbital. 제1항에 있어서, 델타값은 D=Ti,-Tf/Ti+So×K의 방정식에 따라 결정되는 것을 특징으로 하는 측정방법.The method of claim 1, wherein the delta value is determined according to an equation of D = T i , -T f / T i + S o × K. 여기서 D는 델타의 정규화된 값이고; Ti는 샘플의 초기 광학 투과율이고; Tf/T는 샘플의 최종 광학 투과율이고; So는 상기 방법을 수행하기 위해 이용되는 장치에 따른 오프셋이고; k는 미리 결정된 상수이다.Where D is the normalized value of delta; T i is the initial optical transmission of the sample; T f / T is the final optical transmission of the sample; S o is an offset according to the apparatus used to perform the method; k is a predetermined constant. 제1항에 있어서, 상기 피브리노겐 농도 결정 단계는 샘플의 피브리노겐 농도가 R=1og(D/Dc)의 방정식으로 부터 구해지는 R의 값으로 부터 결정되게 하는 상관 방정식을 유도하는 단계와; 유도된 상관 방정식에 따라 플라즈마 샘플의 피브리노겐 농도를 결정하는 단계를 추가로 포함하는 것을 특징으로 하는 측정방법.2. The method of claim 1, wherein determining fibrinogen concentration comprises: deriving a correlation equation such that the fibrinogen concentration of the sample is determined from a value of R obtained from an equation of R = 1og (D / D c ); Determining the fibrinogen concentration of the plasma sample according to the derived correlation equation. 상기 방정식에서, D는 샘플 플라즈마의 델타값이고, Dc는 플라즈마 검정기의 델타값이다.In the above equation, D is the delta value of the sample plasma and D c is the delta value of the plasma analyzer. 제4항에 있어서, 상관 방정식은, 서로 다른 주지된 농도를 잦는 복수의 표준 플라즈마에 대한 델타값을 측정하는 단계와; 주지된 농도를 잦는 플라즈마 검정기에 대한 텔타값을 측정하는 단계와; 복수의 표준 플라즈마에 대하여 Ra대 1og(Cs/Co)를 작도하는 단계에 의해 유도되는 것을 특징으로 하는 측정방법.The method of claim 4, wherein the correlation equation comprises: measuring delta values for a plurality of standard plasmas having different known concentrations; Measuring a delta value for a known plasma concentration at a known concentration; Measuring by R a vs. 1 og (C s / C o ) for a plurality of standard plasmas. 여기서 Rs는 (Ds/Dc)로서, 표준 플라즈마에 대한 R 값이고; Ds는 표준 플라즈마에 대한 측정된 델타값이고; Dc는 플라즈마 검정기에서 대해서 측정된 텔타값이고; Cs는 플라즈마내의 피브리노겐 농도이고; Cc는 플라즈마 검정기내의 괴브리노겐 농도이다.Wherein R s is (D s / D c ), which is the R value for a standard plasma; D s is the measured delta value for the standard plasma; D c is the delta value measured for the plasma assayer; C s is the fibrinogen concentration in the plasma; C c is the concentration of gobrinogen in the plasma assayer. ※참고사항:최초출원 내용에 의하여 공개되는 것임.※ Note: The information is disclosed by the first application.
KR1019920701275A 1989-12-01 1990-11-30 Fibrinogen Direct Analysis Method KR920704117A (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US44394889A 1989-12-01 1989-12-01
US443,948 1989-12-01
PCT/US1990/006988 WO1991008460A1 (en) 1989-12-01 1990-11-30 Direct fibrinogen assay

Publications (1)

Publication Number Publication Date
KR920704117A true KR920704117A (en) 1992-12-19

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Application Number Title Priority Date Filing Date
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Country Link
EP (1) EP0502103A4 (en)
JP (1) JPH05503008A (en)
KR (1) KR920704117A (en)
AU (1) AU641459B2 (en)
CA (1) CA2068221A1 (en)
FI (1) FI922312A0 (en)
IE (1) IE904244A1 (en)
WO (1) WO1991008460A1 (en)
ZA (1) ZA909564B (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5708591A (en) 1995-02-14 1998-01-13 Akzo Nobel N.V. Method and apparatus for predicting the presence of congenital and acquired imbalances and therapeutic conditions
US6429017B1 (en) 1999-02-04 2002-08-06 Biomerieux Method for predicting the presence of haemostatic dysfunction in a patient sample
US6321164B1 (en) 1995-06-07 2001-11-20 Akzo Nobel N.V. Method and apparatus for predicting the presence of an abnormal level of one or more proteins in the clotting cascade
US6898532B1 (en) 1995-06-07 2005-05-24 Biomerieux, Inc. Method and apparatus for predicting the presence of haemostatic dysfunction in a patient sample
US6502040B2 (en) 1997-12-31 2002-12-31 Biomerieux, Inc. Method for presenting thrombosis and hemostasis assay data
EP1147423B1 (en) 1999-02-04 2004-11-10 bioMérieux, Inc. A method and apparatus for predicting the presence of haemostatic dysfunction in a patient sample
US7179612B2 (en) 2000-06-09 2007-02-20 Biomerieux, Inc. Method for detecting a lipoprotein-acute phase protein complex and predicting an increased risk of system failure or mortality
CN110257475B (en) * 2019-06-28 2023-05-02 深圳市国赛生物技术有限公司 Fibrinogen detection reagent, preparation method thereof and detection reagent product

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Publication number Priority date Publication date Assignee Title
US3432268A (en) * 1964-08-28 1969-03-11 Peter Unger Method and apparatus for testing cell suspensions
US3658480A (en) * 1970-04-13 1972-04-25 Bio Data Corp Coagulation timing apparatus, and method
US3833864A (en) * 1972-11-30 1974-09-03 R Kiess Digital direct reading colorimeter
US3861877A (en) * 1974-01-21 1975-01-21 Clinical Technology Inc Optical analysis of fluids
US3905769A (en) * 1974-02-28 1975-09-16 Bagley Wallace E Method and apparatus for measuring prothrombin time and the like
US3989382A (en) * 1975-01-22 1976-11-02 Bio-Data Corporation Platelet aggregation monitoring device
EP0059277A1 (en) * 1981-03-02 1982-09-08 J. & P. Coats, Limited Rapid quantative measurement of fibrinogen in blood plasma
AT382971B (en) * 1981-06-16 1987-05-11 Hoffmann La Roche METHOD AND DEVICE FOR MEASURING THE BLOOD CLUTTING TIME
GB8426004D0 (en) * 1984-10-15 1984-11-21 Ortho Diagnostic Systems Inc Coagulation monitoring
IT1209604B (en) * 1984-11-27 1989-08-30 Instrumentation Lab Spa METHOD AND EQUIPMENT FOR MEASUREMENT OF COAGULATION PARAMETERS.

Also Published As

Publication number Publication date
CA2068221A1 (en) 1991-06-02
AU6898391A (en) 1991-06-26
JPH05503008A (en) 1993-05-27
WO1991008460A1 (en) 1991-06-13
AU641459B2 (en) 1993-09-23
EP0502103A4 (en) 1993-05-05
FI922312A (en) 1992-05-21
IE904244A1 (en) 1991-06-05
ZA909564B (en) 1992-11-25
FI922312A0 (en) 1992-05-21
EP0502103A1 (en) 1992-09-09

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