SU1493187A1 - Method of transplanting plants grown in vitro in cultivation vessels on nutrient medium into non-sterile conditions - Google Patents

Abstract

The invention relates to agriculture, in particular, to plant growing, and can be used in the nursery to produce and propagate healthy planting material. The aim of the invention is to increase the survival rate of plants, reduce time and reduce material costs. The preparation of plants grown in vitro for transplantation under non-sterile conditions is carried out by adapting the plants to environmental conditions. For this, the internal volume of the culture vessel is communicated with the external environment by making a hole in the vessel cover. Through this hole, a solution of the fungicidal preparation is applied to the surface of the nutrient medium, and in no less than 5 days the coating is finally removed from the culture vessel with repeated application of the fungicide preparation to the surface of the nutrient medium. Transplantation to a permanent site is carried out no less than 10 days after removal of the coating from the cultivation vessel. For transplantation, plants with 2–4 roots 3–6 cm long are used. 1 Cp. Of the flesh, 3 tab.

Description

The invention relates to agriculture, in particular, to plant growing, and can be used in the nursery to produce and propagate healthy planting material.

The purpose of the invention is to increase the survival rate of plants, reducing time and reducing material costs.

Plants with two or four roots, 3-6 cm long, rooted in cultivation vessels, are suitable for preparation for transplantation into non-sterile conditions. In the cover of cultivation vessels, a hole of 3-5 mm is punctured, and 3 to 10 millimeters layer: 0.5% solution of systemic fungicide. After 5 days, the coatings from the cultivation vessels are removed completely and the same amount of fungicide solution is applied to the surface of the medium using a pipette. After 10 days, the plants are removed from the cultivation vessels and dive into the mountains.

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shki with soil substrate, pre-washing the root system of plants from the nutrient medium. Then the plants are watered with a solution of complex mineral fertilizers and after 1 day the pots with the plants are transferred to an ordinary greenhouse.

The effectiveness of various methods of transplanting plants grown in vitro in non-sterile conditions is given in Table. one.

The two-stage removal of coatings from cultivation vessels aims to prevent damage to plants from a sharp decrease in air humidity. Otherwise, the plants are drying out. The given time values are the minimum necessary to achieve the goal, i.e. plant adaptation to normal air humidity (50-60%) (see Table 2).

The size of the root system is minimally necessary for 100% survival of plants on a soil substrate. Therefore, it is preferable to take such plants prior to the start of plant preparation for transfer to non-sterile conditions. Plants with root system sizes that are not as described above require additional time to develop the root system of the required size. Since the adaptation of plants in non-sterile conditions, according to the prototype, is carried out at 100% moisture, the size of the root system in the initial stage of preparation does not play a significant role. Water transpiration by plant leaves under such conditions is practically absent. In the proposed method, the root system of the specified size provides the necessary water supply for the plants. Thus, the limiting factor is the level of water transpiration from plant leaves.

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Data survival of plants, depending on the number of roots and their length, are given in table. 3

Preparing plants grown in tissue culture for transplanting to a permanent place can increase the survival rate of plants in non-sterile conditions, and reduces the time and labor costs for the process of plant adaptation to environmental conditions.

Claims (2)

1. A method of transplanting plants grown in vitro on a nutrient medium in coated cultivation vessels under non-sterile conditions, including adapting plants to environmental conditions and transplanting to a permanent growth site, characterized in that, in order to increase the survival rate of plants , reduce time and reduce material costs, the adaptation of plants to environmental conditions is carried out directly in a cultivation vessel on a nutrient medium, for which the internal volume of the cultivation vessel communicates with the external environment Putting a hole in the cover of the cultivation vessel and applying the fungus preparation solution is applied to the surface of the nutrient medium, and after not less than 5 days the coating is removed from the ijjioHHoro culture vessel with repeated application of the fungicide preparation to the surface of the nutrient medium, and transplanting to a permanent growth site, no less than 10 days after removal of the coating from the cultivation vessel. 2. The method according to claim 1, with tl and h a 45 u and so that for transplantation plants are used, and a length of 3-6 cm. having 2-4 boxes 2 Reda torus N.Kystulinets Compiled by S. Kunayeva Tehred L. Oliinzh Order 3904/4 Circulation 621 VNIIPI State Committee for Inventions and Discoveries at the State Committee on Science and Technology of the USSR 113035, Moscow, Zh-35, Raushsk nab. 4/5 Production and publishing plant Patent, Uzhgorod, st. Gagarin, 101 Table 3 Proofreader M.Samborsk Subscription