SU1293192A1 - Method of preparing alkaline soils for rice seeding - Google Patents

Abstract

The invention relates to the field of agriculture, in particular to methods for increasing the yield and increasing the biological quality of rice grains during reclamation of alkaline soils. The aim of the invention is to increase the amylase activity of germinating rice StepHa. The method consists in carrying out agrotechnical measures, application of mineral fertilizers to the soil, zinc nitrate in the amount of 200 kg / ha, and simultaneous application of 5-7 t / ha of rice straw inoculated with Lactobacterium plantarum bacteria with a titer of cells per gram of straw. The method allows simultaneously increasing the yield of rice on alkaline soils to 36.2 c / ha and the germination of the obtained grain to 93.0% due to an increase in the about-amylase activity of the germinating grain. 3 tab. with SO y with co with N)

Description

f5

112931922

The invention relates to agricultural Kuban-3 variety cultivated in the agricultural sector, in particular, to alkaline alluvial-meadow non-abundant increase in yields and povnelenny soil containing 2.3% of the total biological, rice grain quality at reclamation of alkaline soils.

The purpose of the invention is the growth of the oh-amylase activity of the germinating rice grain.

Example 1, Germination energy of rice seeds obtained by Melio-O Zn (NO,) 2 .; 6 - straw + Zn (NO). In the case of zinc nitrate, Zn (NOj) 2, inoculation of straw with lactic acid and straw, inoculated with lactic acid bacteria Lactobacterium plantarum strain 3 sec (ICD) with a titer of U - Uc / g of straw, alluvial-meadow soil, was checked by setting model experiment in laboratory conditions, Petri dishes are seeded with 20 seeds per

options: control; straw 5-7 t / ha; 0 Active ingredient zinc straw + MB; ZnCNO,) 200 kg / ha; Zn (NO,), 1 + straw and Zn (NOg), + straw + ICD, The cups are poured with a layer of water of 0.3 cm and kept in a thermostat at 28 ° C for 3 days. The experiment is set in 5 replicates. The correlation between the oi-amylase activity of the germinating rice grain and the germination energy is determined.

Germination energy and oi-amylazgumus, pH 8.6, Experience consists of the following options: 1 - control (); 2 - rice straw was introduced (6.5 t / ha); 3 - straw treated with lactic acid bacteria (tKB) 4 - straw + ICD + ZnCNO,); 5 bacteria (12 10 ° cells per 6.5 tons of straw) is used pure culture of Lactobacterium plantarum strain 3c, isolated in the feed microbiology laboratory of the Institute of Microbiology and Virology of the Academy of Sciences of the Kazakh Academy of Sciences, SSR,

Mineral fertilizers used in the form of ammonium sulfate and superphosphate25

thirty

40 kg / ha. The determination of the activity of the total preparation amylase and o4-amylase is carried out according to the methods adopted in the laboratory of grain biochemistry of the Institute of Molecular Biology and Biochemistry of the Academy of Sciences of the Kazakh SSR.

Studies show that, in comparison with the known method, the proposed method significantly increases the content in the soil of mobile forms of phosphorus and potassium, contributes to the formation of fuller grains (4-10%), leads to an increase in rice yield of 71-138%,

The activity of rice seeds obtained on reclaimed alluvial soil is presented in Table. one.

The correlation coefficient between the value of oi-amylase activity and seed germination for 3 days. germination is 0.95. Thus, there is a very well-pronounced positive correlation between the germination of rice seeds and the activity of α-amylase. Seeds obtained against the background of Zn (NO) + straw + ICD are 39–16% more active than those treated with nitric acid. Rat Zinc (300 kg / ha).

Up to 5-7 t / ha of straw is selected from the average straw productivity in rice-planting zones of the USSR, as well as from experience showing that adding 7 t / ha of straw is the limit for embedding into the soil.

The titer of lactic acid bacteria 10 cells / g of straw is selected from the condition

the greatest formation of organic 55 growing rice grain, depending on

acids in this range, from land-improvement ameliorants, "

Example 2, Cases night experiments For the grain obtained by production on the Akdalinsky water background (N, 4o) harak, the irrigation massif of the Balkhash region, the thorns are high in activity, α-amylases.

Kuban-3 rice is grown on alkaline alluvial-meadow non-salted soil containing 2.3% of the total

Zn (NO,) 2 .; 6 - straw + Zn (NO). When inoculation of straw with lactic acid

humus, pH 8.6, Experience consists of the following options: 1 - control (); 2 - rice straw was introduced (6.5 t / ha); 3 - straw treated with lactic acid bacteria (tKB); 4 - straw + ICD + ZnCNO,); five

Zn (NO,) 2 .; 6 - straw + Zn (NO). When inoculation of straw with lactic acid

 Zinc active ingredient

bacteria (12 10 ° cells on 6.5 tons of straw) is used pure culture of Lactobacterium plantarum strain 3c, isolated in the feed microbiology laboratory of the Institute of Microbiology and Virology of the Academy of Sciences of the Kazakh Academy of Sciences, SSR,

Mineral fertilizers used in the form of ammonium sulfate and superphosphate. 0 Active ingredient zinc.

five

0

five

0 5

0

40 kg / ha. The determination of the activity of the totale preparation amylase and o4-amylase is carried out according to the methods adopted in the laboratory of biochemistry of grain crops of the Institute of Molecular Biology and Biochemistry of the Academy of Sciences of the Kazakh SSR.

Studies show that, in comparison with the known method, the proposed method significantly increases the content in the soil of mobile forms of phosphorus and potassium, contributes to the formation of fuller grains (4-10%), leads to an increase in rice yield of 71-138%,

The germination efficiency of grains in t HoroM is determined by amylase activity. In addition, the activity level of this protein-synthesizing system can be judged from the level of activity of this enzyme during germination of grain, since about 30-50% denovo synthesized protein is oi-amylase. Thus, the association of the amylase activity with the viability of the grain, i.e., its biological quality, becomes obvious.

The content of nutrients in the alluvial-meadow soil and the yield of rice with the introduction of various ameliorants are presented in Table 2.

Table 3 shows data on the variability of the total (- and o-amylase) and on the-amylase activity of pro312

use - as an organic fertilizer of rice straw reduces it somewhat (6-7%). Treatment of straw with a culture of lactic acid bacteria and thus caused changes in the plant nutritional regimen leads to a decrease in oi-amylase activity by almost half. The alkaline reaction of the medium and the high content of zinc salts impede the processes of respiration and photosynthesis of plants and sometimes irreversibly affect them.

In this connection, the effect of zinc salts on the amylase of rice grains is indicative. On alumina-arc soil, the use of zinc nitrate leads to a sharp decrease in the activity of both the total drug and c6-amylase. Only joint application of zinc salts with organic fertilizers allows to increase this

14 8

KB 17

13 8

sixteen

16 12

17

24

most important indicator. Of greatest interest is the variant with the introduction of Zn (NO) + straw + zhB, which is characterized by high activity of α-amylase, yield, mass of 1000 grains and the content of nutrients.

Claims (1)

Invention Formula A method of preparing alkaline soils for sowing rice, including carrying out agrotechnical measures, introducing mineral fertilizers and zinc nitrate into the soil, characterized in that, in order to increase the od-amylase activity of the germinating grain, 5 zinc is added to the soil -7 t / ha of rice straw inoculated with Lactobacterium planta rum No. 3 bacteria strain with a titer of 10 -10 cells / g of straw, Table 1 14 ; one 18 13.8 ± 2 10 + 2 16.6 +., 4 100 91 69 77 56 93 100 92.2 50 66.5 31.7 94.3 N, 40% Straw 6.5 t / ha Straw-5- RC Zn (W  gp (Shz) 2 + straw Zn (NO,) 2 + straw + ICD Control options 107.6 100 16.6 100 161 100 25.510015.2100 145,4135,111,0 66,2 185 114,9270105,926,9170,0 Known method 169.5157.5 11.2. 67.4 190 118.0 27.1106,540.02.0260.3 156.3 145.2 15.1 90.9 154 95.6 27.6108.225.6167.0 160,0148,617,5 105,4179 111,127,4107,428,3186.0 The proposed method 155.6144.6 21.7 130.7 214 132.9 28,0109,836,2238.2 Table 3