CN112574903A - Complex microbial inoculant and application thereof - Google Patents

Complex microbial inoculant and application thereof Download PDF

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CN112574903A
CN112574903A CN202010741141.4A CN202010741141A CN112574903A CN 112574903 A CN112574903 A CN 112574903A CN 202010741141 A CN202010741141 A CN 202010741141A CN 112574903 A CN112574903 A CN 112574903A
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bacillus
fertilizer
microbial inoculum
mucilaginosus
powder
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杜延全
满丽萍
柴彦亮
战帅
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Sinofert Holding Ltd
Sinochem Agriculture Linyi Research and Development Center Co Ltd
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Sinochem Agriculture Linyi Research and Development Center Co Ltd
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    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
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    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
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    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
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Abstract

The invention provides a compound microbial inoculum. The composite microbial inoculum comprises bacillus mucilaginosus, bacillus subtilis and bacillus laterosporus. The composite microbial inoculum can effectively promote the root growth of monocotyledon crops and improve the yield.

Description

Complex microbial inoculant and application thereof
Technical Field
The invention relates to the field of fertilizers, in particular to a compound microbial inoculum and application thereof.
Background
Microorganisms have been widely used in agricultural production, known as PRPG, and are commonly applied to crop seeds or soil in the form of microbial fertilizers, biological agents, functional additives, seed dressings, and the like. The microorganism living preparation prepared from azotobacter, rhizobium, photosynthetic bacteria, potassium bacteria, phosphorus bacteria and the like can form a root system microenvironment through interaction with rhizosphere in the microorganism metabolic process, such as release of gibberellin, heteroauxin, disease-resistant growth-promoting enzymes and the like, promote the growth and development of crops, and reduce plant diseases and insect pests.
The bacillus mucilaginosus is commonly called silicate bacteria, has a dissolving and utilizing function on insoluble minerals in soil, can promote the release of mineral elements in the soil, is utilized during self growth and propagation, and can also promote the biogeochemical cycle of the mineral elements in the soil, such as silicon cycle, so as to promote the growth and development of silicon-loving plants. The bacillus subtilis has a good effect on preventing and treating diseases of crops such as rice, wheat and the like, has strong nutrient substance competitive capacity and oxygen competitive advantage, and plays a role in protecting healthy growth of crops. The bacillus laterosporus also has better effects on the aspects of inhibiting the propagation of pathogenic bacteria, promoting the growth of plant root systems and improving the quality of crops.
Because monocotyledons and dicotyledons have different structures such as root systems, embryos, cotyledons and the like, the nutrition requirements are different. Monocotyledon root systems are relatively dispersed and shallow, and compared with large root systems, the microbial inoculum is fast attached to the surface of the root system after being propagated, and a rhizosphere effect can be fast formed.
The screening of the monocotyledon growth promoting microbial inoculum is carried out in a targeted manner, so that the application of the microbial inoculum in the planting process of monocotyledon crops such as wheat and rice can be enhanced.
Disclosure of Invention
The present invention is directed to solving, at least to some extent, one of the technical problems in the related art.
In a first aspect of the invention, the invention provides a complex microbial inoculant. According to an embodiment of the invention, the complex microbial inoculum comprises bacillus mucilaginosus, bacillus subtilis and bacillus laterosporus. The composite microbial inoculum according to the embodiment of the invention can effectively promote the root growth of monocotyledon crops and improve the yield.
According to the embodiment of the invention, the complex microbial inoculum can further comprise at least one of the following additional technical characteristics:
according to an embodiment of the present invention, the bacillus mucilaginosus, the bacillus subtilis and the bacillus laterosporus are provided in the form of bacterial powder.
According to the embodiment of the invention, the effective viable count of the bacillus mucilaginosus powder is 1 multiplied by 107～1.5×1010CFU/g, the effective viable count of the bacillus subtilis powder is 1 multiplied by 107～3×1011CFU/g, the effective viable count of the lateral spore bacillus powder is 1 multiplied by 107～3×1010CFU/g。
According to the embodiment of the invention, the mass ratio of the bacillus mucilaginosus powder to the bacillus subtilis powder to the bacillus laterosporus powder is (1-1.2): (1-1.2) and (0.5-1).
According to the embodiment of the invention, the number ratio of the effective viable count of bacillus mucilaginosus, the effective viable count of bacillus subtilis and the effective viable count of bacillus laterosporus is 1: (5X 10)-4～3.6×103)：(2.7×10-4～3 ×103) Preferably, 1: (20-30): (0.5 to 5), more preferably, 1: 20: 2. the inventor finds that the growth promoting effect of the composite microbial agent is more obvious when the viable bacteria amount of the bacillus mucilaginosus, the bacillus subtilis and the bacillus laterosporus is within the proportion range.
According to the embodiment of the invention, based on the mass of the composite microbial inoculum, the effective viable count of the composite microbial inoculum is 1 x 107～1.3*1011CFU/g, preferably, 1 x 107～1.15*1011CFU/g。
According to an embodiment of the present invention, the bacillus mucilaginosus, the bacillus subtilis and the bacillus laterosporus are obtained after fermentation treatment, and the fermentation treatment is performed in the following way: fermenting the seed liquid for 9-15 hours in advance; and continuing fermentation treatment on the seed liquid, wherein the bacillus mucilaginosus is obtained after continuing fermentation for 40-48 hours, the bacillus subtilis is obtained after continuing fermentation for 30-36 hours, and the bacillus laterosporus is obtained after continuing fermentation for 30-36 hours. The inventor finds that more than 90% of bacillus mucilaginosus, bacillus subtilis and bacillus laterosporus are sporulated after the fermentation in the time, and the growth promoting effect of the composite microbial inoculum is more remarkable.
According to the embodiment of the invention, the preservation number of the bacillus mucilaginosus is CGMCC No.19585, the preservation number of the bacillus subtilis is CGMCC No.19584, and the preservation number of the bacillus laterosporus is CGMCC No. 19583.
According to the embodiment of the invention, the preservation number of the bacillus mucilaginosus is CGMCC No.19585, the preservation unit is as follows: china general microbiological culture Collection center, the preservation unit address: the microbial research institute of western road No.1, 3, china academy of sciences, bei yang district, jing city, the storage day: 17/04/2020, category name: bacillus mucilaginosus; the preservation number of the bacillus subtilis is CGMCC No.19584, and the preservation unit is as follows: china general microbiological culture Collection center, the preservation unit address: the microbial research institute of western road No.1, 3, china academy of sciences, bei yang district, jing city, the storage day: 17/04/2020, category name: bacillus subtilis; the preservation number of the bacillus laterosporus is CGMCC No.19583, and the preservation unit is as follows: china general microbiological culture Collection center, the preservation unit address: the microbial research institute of western road No.1, 3, china academy of sciences, bei yang district, jing city, the storage day: 17/04/2020, category name: bacillus laterosporus.
In a second aspect of the invention, a fertilizer is provided. According to the embodiment of the invention, the fertilizer comprises the compound microbial inoculum. The fertilizer provided by the embodiment of the invention can obviously promote the root growth of monocotyledon crops and improve the yield.
According to an embodiment of the present invention, the fertilizer may further comprise at least one of the following additional technical features:
according to an embodiment of the invention, the fertilizer is a compound fertilizer, an organic fertilizer or an organic-inorganic fertilizer.
According to the embodiment of the invention, the effective viable count of the compound microbial inoculum is 0.5 x 10 based on the quality of the fertilizer8CFU/g ～1*108CFU/g。
In a second aspect of the invention, a method of growing a monocot plant is provided. According to an embodiment of the present invention, the method comprises applying the complex microbial inoculum or the fertilizer as described above to the roots of the monocotyledonous plant. According to the cultivation method provided by the embodiment of the invention, the growth promotion effect on the monocotyledons is remarkable, the root growth of the monocotyledons can be remarkably promoted, and the yield of the monocotyledons is improved.
According to an embodiment of the present invention, the method may further include at least one of the following additional technical features:
according to an embodiment of the invention, the monocotyledonous plant is rice or wheat. The growth promoting effect of the cultivation method provided by the embodiment of the invention on rice or wheat is more obvious.
According to an embodiment of the invention, the fertilizer is applied in an amount of 40-60 kg/acre, preferably 50 kg/acre.
The compound microbial inoculum provided by the invention has wide application mode, can be independently used as a microbial inoculum in an agricultural planting process, and can also be applied to fertilizer production to prepare biological fertilizers by adding organic fertilizers, compound fertilizers and organic and inorganic fertilizers.
Compared with the prior art, the invention has the beneficial effects that:
1. a series of effect tests prove that the selected microbial strains and the prepared composite microbial inoculum have obvious promotion effects on root systems and plant yields of monocotyledons including wheat and rice.
2. The selected microbial strains are strains screened in natural environment, have high-efficiency growth promoting capacity, do not generate antagonism among the strains, and can coexist in soil.
3. The selected microbial strains have good colonization effect in soil and can quickly form probiotic groups.
4. The microbial agent can promote the development of crop roots and improve the freeze injury resistance of wheat.
Detailed Description
The following detailed description of the embodiments of the present invention is intended to be illustrative, and not to be construed as limiting the invention.
Example 1 Process for preparing a monocot growth-promoting Complex microbial inoculum
The invention relates to a monocotyledon growth promoting type composite microbial inoculum which comprises bacillus mucilaginosus, bacillus subtilis and bacillus laterosporus. The preparation method comprises the following specific steps:
step 1: a solid slant culture medium is prepared by adopting a slant culture mode, bacillus mucilaginosus (with the preservation number of CGMCC No.19585) is inoculated on the solid slant of a silicate bacteria culture medium, and bacillus subtilis (with the preservation number of CGMCC No.19584) and bacillus laterosporus (with the preservation number of CGMCC No.19583) are respectively inoculated on the solid slant of an LB culture medium.
Step 2: seed tank fermentation culture: respectively inoculating the activated bacillus mucilaginosus, the activated bacillus subtilis and the activated bacillus laterosporus in the step 1 into a seed fermentation tank, and setting different fermentation parameters.
And step 3: expanding fermentation: and (3) respectively adding the bacillus mucilaginosus seed liquid, the bacillus subtilis seed liquid and the bacillus laterosporus seed liquid which are fermented in the step (2) into a fermentation tank, and setting different fermentation parameters to obtain fermentation liquid.
And 4, step 4: respectively drying the bacillus mucilaginosus fermentation liquor, the bacillus subtilis fermentation liquor and the bacillus laterosporus fermentation liquor obtained in the step 3 through a spray drying system to respectively obtain bacillus mucilaginosus powder, bacillus subtilis powder and bacillus laterosporus powder, wherein the effective viable count of the bacillus mucilaginosus powder is 1 multiplied by 10 through measurement7～1.5×1010CFU/g, the effective viable count of the bacillus subtilis powder is 1 multiplied by 107～3×1011CFU/g, the effective viable count of the lateral spore bacillus powder is 1 multiplied by 107～3×1010CFU/g。
And 5: and (3) mixing the dried bacterial powder in a proportion of 1-1.2: and (3) mixing the materials in a ratio of 1-1.2: 0.5-1 to obtain various samples of the composite microbial inoculum.
The fermentation conditions, mixing ratio, effective viable count and the like of various samples of the microbial inoculum are shown in the table below.
Table 1: proportion of complex microbial inoculum and fermentation conditions
Figure RE-GDA0002948313900000041
Figure RE-GDA0002948313900000051
Example 2 plate growth promotion test of different microbial inoculum for wheat and rice
The bacillus mucilaginosus, the bacillus subtilis and the bacillus laterosporus which are related in the invention are taken as test strains, the bacteria liquid is prepared according to the step 3 of the embodiment 1, wherein the composite bacteria liquid is the bacillus mucilaginosus, the bacillus subtilis and the bacillus laterosporus which are mixed according to the proportion shown in the table 1, and the wheat seed and rice seed plate growth promotion tests are respectively carried out by the same amount of fermentation liquid according to the comparative examples of other bacteria agents, and the results show that the three strains have better root growth promotion effects on wheat and rice than other strains, and the plate effect after the combination is better than that of the single use.
When the bacillus mucilaginosus, the bacillus subtilis and the bacillus laterosporus are used independently, the main root length of the rice is increased by 50 percent, 54 percent and 62 percent relative to that of the rice in a blank test group, the main root length is increased by 30 to 66 percent compared with that of other microbial inoculums, and the germination rate is increased by 9 to 20 percent; the main root length of the compound microbial inoculum is increased by 68 percent relative to that of a blank test group, is increased by 46 to 72 percent compared with that of other microbial inoculants, and the germination rate is increased by 18 to 22 percent.
When the bacillus mucilaginosus, the bacillus subtilis and the bacillus laterosporus are used independently, the main root length of wheat in a blank test group is increased by 45 percent, 31 percent and 33 percent respectively, and the germination rate is increased by 4 to 21 percent; the growth rate is increased by 19 to 46 percent compared with other microbial inoculum; the main root length of the compound microbial inoculum is increased by 50 percent relative to that of a blank test group, is increased by 38 to 52 percent compared with that of other microbial inoculants, and the germination rate is increased by 13 to 23 percent.
The data of the wheat and rice growth promoting test on the plate are shown in the table 2.
Table 2: results of plate growth promotion test among different microbial agents
Figure RE-GDA0002948313900000052
Figure RE-GDA0002948313900000061
As can be seen from Table 2, the plate growth promoting effect of the three bacteria on rice or wheat after being compounded is obviously superior to that of the three bacteria which are independently applied, and the growth promoting effect of the compound microbial inoculum 1 and 6 is superior to that of the compound microbial inoculum 3.
Example 3 wheat and rice potting test comparing the effects of the composite microbial inoculum with the effects of a single microbial inoculum
The compound microbial inoculum compound samples of numbers 1-6 prepared in the example 1 are used as test raw materials, the single microbial inoculum of bacillus licheniformis, bacillus megaterium and bacillus amyloliquefaciens and the three market compound microbial inoculants are used as references, rice and wheat are used as test crops respectively, and 30-day pot culture is carried out, and the results show that compared with a blank test group, the microbial inoculum disclosed by the invention can be used for increasing the main root length of rice by 48-56%, and compared with the single microbial inoculum and the compound microbial inoculum which are externally harvested, the main root length of rice is increased by 21-59%. Compared with a blank test group, the microbial inoculum disclosed by the invention can increase the main root length of wheat by 15% -29%, and compared with a single microbial inoculum and a compound microbial inoculum which are collected externally, the main root length of rice is increased by 11% -35%. Therefore, the composite microbial inoculum has obvious advantages in the aspect of promoting root length. The data of the wheat and rice pot experiment are shown in the table 3.
Table 3: test results of growth promotion of rice and wheat potted plants
Figure RE-GDA0002948313900000062
Figure RE-GDA0002948313900000071
As can be seen from Table 3, the growth promoting effect of the three bacteria on the rice or wheat potting after being compounded is obviously superior to the growth promoting effect of the three bacteria when being independently applied and the microbial inoculum is applied in the market.
Example 4 synergistic effect of monocot growth-promoting type complex microbial inoculum in wheat and rice field
The compound microbial inoculum compound samples of numbers 1-6 prepared in the example 1 are respectively used as test raw materials, single microbial inoculants of bacillus licheniformis, bacillus megaterium and bacillus amyloliquefaciens and three market compound microbial inoculants are used as references, rice and wheat are respectively used as test crops, and field tests of rice and wheat are carried out in Hubei province and Henan province, and the results show that the microbial inoculants can increase the yield of rice by 28-35% relative to a blank test group, and increase the yield of rice by 21-27% relative to the single microbial inoculants and the compound microbial inoculants which are externally collected; compared with a blank test group, the microbial inoculum can increase the yield of wheat by 21-36 percent, and the yield of rice by 9-26 percent compared with a single microbial inoculum and a compound microbial inoculum which are externally collected. Therefore, the composite microbial inoculum has obvious advantages in the aspect of increasing the yield. The data of the wheat and rice field test are shown in table 4.
Table 4: yield increasing in rice and wheat field
Figure RE-GDA0002948313900000072
Figure RE-GDA0002948313900000081
As can be seen from Table 4, the field yield increasing effect of the compound of the three bacteria on rice or wheat is obviously better than the field yield increasing effect of the single application of the three bacteria and the application of the commercially available microbial inoculum, wherein the field yield increasing effect of the compound microbial inoculum 1 is optimal.
Example 5 field synergistic Effect of a monocot plant growth promoting type Complex microbial inoculum of wheat organic and inorganic fertilizers
The composite microbial agent, the bacillus mucilaginosus microbial agent, the bacillus subtilis microbial agent and the bacillus laterosporus microbial agent prepared in the example 1 are respectively coated on the surfaces of 15-40(20-13-7) of organic and inorganic fertilizers with high inorganic nutrients, so that the effective viable count is 0.5 x 108 CFU/g～1*108CFU/g CFU/g, and the biological organic and inorganic fertilizer is obtained. And (3) performing a wheat field fertilizer efficiency test by taking the organic and inorganic fertilizers without the added microbial inoculum as a control group. The fertilizer application amount of the test group and the fertilizer application amount of the control group are the same, and are respectively 50 kg/mu, 3 times of the fertilizer application amount are respectively arranged, and the fertilizer application amount is randomly arranged in blocks. The results of field tests show that after the monocotyledon growth promoting type composite microbial inoculum is added into an organic and inorganic fertilizer, the yield of wheat can be obviously improved, and under the condition of equal application amount, the biological organic and inorganic fertilizer added with the monocotyledon growth promoting type composite microbial inoculum is respectively 30-32.9% higher than CK subjected to fertilization treatment and 13-15.5% higher than NPK subjected to conventional fertilization treatment, 5.5-8% higher than organic and inorganic fertilizer without microbial inoculum according to the same product specification, and 3.1-6.0% higher than the organic and inorganic fertilizer added with a single microbial inoculum.
The measured production data are shown in the following table.
Table 5: wheat yield statistical table
Figure RE-GDA0002948313900000082
Figure RE-GDA0002948313900000091
As can be seen from Table 5, the field synergistic effect of the biological organic and inorganic fertilizer wheat wrapped with the composite microbial inoculum is obviously better than the field yield increasing effect of the biological organic and inorganic fertilizer wrapped with the single microbial inoculum and the field yield increasing effect of the organic and inorganic fertilizer wheat without the microbial inoculum, wherein the field synergistic effect of the biological organic and inorganic fertilizer wheat wrapped with the composite microbial inoculum 1 or 6 is better than the field synergistic effect of the biological organic and inorganic fertilizer wheat wrapped with the composite microbial inoculum 3.
Example 6 Rice field Effect-increasing effects of a Monocotyledon growth-promoting Complex microbial inoculum
The surface of 40-20 organic and inorganic fertilizers is respectively coated with the composite microbial agent, the bacillus mucilaginosus microbial agent, the bacillus subtilis microbial agent and the bacillus laterosporus microbial agent prepared in the example 1, so that the number of effective viable bacteria is 1 hundred million CFU/g, and the biological organic and inorganic fertilizer is formed. And (4) performing a rice field fertilizer efficiency test by taking the organic and inorganic fertilizers without the added microbial inoculum as a control group. The fertilizer application amount of the test group and the fertilizer application amount of the control group are the same, and are respectively 30 kg/mu, 3 times of the fertilizer application amount are respectively arranged, and the fertilizer application amount is randomly arranged in blocks. The results of field tests show that after the monocotyledon growth promoting type composite microbial inoculum is added into an organic and inorganic fertilizer, the yield of rice can be obviously improved, and under the condition of equal application amount, the biological organic and inorganic fertilizer added with the monocotyledon growth promoting type composite microbial inoculum is respectively 87.8-88.5% higher than CK subjected to fertilization treatment and 35.7-36.2% higher than NPK subjected to conventional fertilization treatment, 11.1-11.5% higher than the organic and inorganic fertilizer added with microbial inoculum in different specifications, and 2.2-2.7% higher than the organic and inorganic fertilizer added with a single microbial inoculum.
The measured production data are shown in the following table.
Table 6: rice yield statistical table
Figure RE-GDA0002948313900000092
Figure RE-GDA0002948313900000101
As can be seen from Table 6, the field synergistic effect of the biological organic and inorganic fertilizer rice wrapped with the composite microbial inoculum is obviously better than the field yield increasing effect of the biological organic and inorganic fertilizer wrapped with the single microbial inoculum and the organic and inorganic fertilizer without the microbial inoculum.
In the description herein, references to the description of the term "one embodiment," "some embodiments," "an example," "a specific example," or "some examples," etc., mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above are not necessarily intended to refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples. Furthermore, various embodiments or examples and features of different embodiments or examples described in this specification can be combined and combined by one skilled in the art without contradiction.
Although embodiments of the present invention have been shown and described above, it is understood that the above embodiments are exemplary and should not be construed as limiting the present invention, and that variations, modifications, substitutions and alterations can be made to the above embodiments by those of ordinary skill in the art within the scope of the present invention.

Claims (10)

1. A composite microbial inoculum is characterized by comprising bacillus mucilaginosus, bacillus subtilis and bacillus laterosporus.
2. The complex microbial inoculant according to claim 1, wherein the bacillus mucilaginosus, bacillus subtilis and bacillus laterosporus are provided in the form of a bacterial powder;
optionally, the effective viable count of the Bacillus mucilaginosus powder is 1 × 107～1.5×1010CFU/g, the effective viable count of the bacillus subtilis powder is 1 multiplied by 107～3×1011CFU/g, the effective viable count of the lateral spore bacillus powder is 1 multiplied by 107～3×1010CFU/g；
Optionally, the mass ratio of the bacillus mucilaginosus powder to the bacillus subtilis powder to the bacillus laterosporus powder is (1-1.2): (1-1.2) and (0.5-1).
3. The complex microbial agent according to claim 1, wherein the number of effective viable bacteria of Bacillus mucilaginosus, the number of effective viable bacteria of Bacillus subtilis, and the number of effective viable bacteria of Bacillus laterosporusThe ratio is 1: (5X 10)-4～3.6×103)：(2.7×10-4～3×103) Preferably, 1: (20-30): (0.5-5).
4. The complex microbial inoculant according to claim 1, wherein the effective viable count of the complex microbial inoculant is 1 x 10 based on the mass of the complex microbial inoculant7～1.3*1011CFU/g。
5. The complex microbial inoculum of claim 1, wherein the bacillus mucilaginosus, the bacillus subtilis and the bacillus laterosporus are obtained after fermentation treatment, and the fermentation treatment is carried out by the following way:
fermenting the seed liquid for 9-15 hours in advance;
the seed liquid is continuously fermented,
wherein the Bacillus mucilaginosus is obtained after fermentation for 40-48 hours,
the bacillus subtilis is obtained after 30-36 hours of fermentation is carried out,
the bacillus laterosporus is obtained after fermentation is carried out for 30-36 hours.
6. The composite bacterial agent as claimed in claim 1, wherein the preservation number of Bacillus mucilaginosus is CGMCC No.19585, the preservation number of Bacillus subtilis is CGMCC No.19584, and the preservation number of Bacillus laterosporus is CGMCC No. 19583.
7. A fertilizer, characterized by comprising the complex microbial inoculum according to any one of claims 1 to 6.
8. The fertilizer according to claim 7, wherein the fertilizer is a compound fertilizer, an organic fertilizer or an organic-inorganic fertilizer.
9. Fertilizer according to claim 7, characterized in that, based on the mass of the fertilizer,the effective viable count of the composite microbial inoculum is 0.5 x 108CFU/g～1*108CFU/g。
10. A method for cultivating a monocotyledonous plant, which comprises applying the complex microbial agent of any one of claims 1 to 6 or the fertilizer of any one of claims 7 to 9 to the root of a monocotyledonous plant;
optionally, the monocot is rice or wheat;
optionally, the fertilizer is applied at a rate of 40-60 kg/acre, preferably 50 kg/acre.
CN202010741141.4A 2020-07-17 2020-07-17 Complex microbial inoculant and application thereof Pending CN112574903A (en)

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CN114437980A (en) * 2022-02-09 2022-05-06 山东农业大学 Microbial agent and application thereof

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CN110330372A (en) * 2019-06-19 2019-10-15 湖北中化东方肥料有限公司 Shrimp rice field special bio compound organic and inorganic fertilizer and preparation method thereof

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110330372A (en) * 2019-06-19 2019-10-15 湖北中化东方肥料有限公司 Shrimp rice field special bio compound organic and inorganic fertilizer and preparation method thereof

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114437980A (en) * 2022-02-09 2022-05-06 山东农业大学 Microbial agent and application thereof
CN114437980B (en) * 2022-02-09 2023-11-24 山东农业大学 Microbial agent and application thereof

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