SK279821B6 - Amine oxidic disinfectants containing isopropyl alcohol - Google Patents

Abstract

Disclosed are disinfectants based on long-chain linear or branched alkyldimethylamineoxides with the content of isopropyl alcohol which serves the function of an excipient exhibiting antimicrobial activity. Organic ammonium salt, chlorhexidine and glycerol are further components. Amine oxide, organic ammonium salt and chlorhexidine provide a wide spectrum and stability of antimicrobial activity together with detergent and penetration activity; and glycerol prevents the skin from dehydrating. Isopropyl alcohol gives potential and widens the antimicrobial activity of basic components. This composition can be used for instant disinfecting of hands, skin and other surfaces in the health service, communal hygiene, food-processing industry and agriculture, etc..

Description

SK 279821 Β6

Technical field

The invention relates to disinfectants comprising an amine oxide and other active ingredients soluble in an aqueous solution of isopropyl alcohol. Isopropyl alcohol in addition to its own antimicrobial activity, potency and extended antimicrobial effect of surface-active alkyl dimethyl branched or linear with the number of carbon atoms in the chain C _w and C _g. These disinfectants are intended for use in the rapid disinfection of hands, skin and other surfaces in healthcare, municipal hygiene, food and agriculture.

CH ₃

BACKGROUND OF THE INVENTION

Disinfectants containing surface-active amine oxides are known, but the onset of action is slow and the spectrum of microorganisms on which these agents act is relatively narrow. However, their advantages are low acute toxicity, low side effects, compatibility with a wide range of compounds used in this field, relatively low skin and eye irritation, and surface activity, potentiating the effect of other disinfectants. Therefore, it is advantageous to use them together with more vigorous and more drastic substances, while in the resulting combinations the advantages of the individual components can be exploited while at the same time reducing their drawbacks.

Aliphatic alcohols are known as disinfectants with limited antimicrobial activity and are often used as solvents for others, e.g. cosmetic or pharmaceutical products. However, several of these alcohols are unsuitable for wider use, e.g. for high toxicity to humans. Isopropyl alcohol is the most preferred of the water-miscible alcohols because it is the most effective antimicrobial. Isopropyl alcohol disinfectants are considered the best practice for hand disinfection. However, isopropyl alcohol also has a number of disadvantages. After its evaporation, which is relatively fast, the antimicrobial effect rapidly subsides and the treated surfaces are easily recontaminated. Its spectrum of action is also not satisfactory. When applied to the skin, it degreases and dehydrates. These drawbacks can be overcome by suitably combining it with other antimicrobial compounds and optionally other ingredients.

SUMMARY OF THE INVENTION

SUMMARY OF THE INVENTION The present invention is based on the formulation of a combination antimicrobial composition intended for topical application to uninjured skin as well as for the disinfection of various surfaces or materials. The present invention provides a combination of a plurality of antimicrobial active compounds in an isopropyl alcohol vehicle. The composition is comprised of 0.1 to 1.0 wt. amine oxide of formula R (CH _{3) 2} N ⁺ H ', and the structure

_CH3 wherein R) is a linear or branched, substituted or unsubstituted alkyl chain with carbon number 12 to 16 and R ₂ is either a methyl or a benzyl radical and X is a halide anion, and, finally, 0.1 to 1.0% by weight. chlorhexidine in 70% v / v of an aqueous solution of isopropyl alcohol and contains up to 1,0% vol. glycerol. The individual components, with the exception of glycerol, have their own spectrum of antimicrobial activity and other specific properties. By combining these mutually compatible compounds, their advantages are optimally exploited while at the same time their individual disadvantages are eliminated. They broaden their spectrum of action on Gram-positive and Gram-negative bacteria, microscopic fungi and enveloped viruses, and achieve a synergistic increase in the efficacy of the whole formulation, which is ultimately higher than that of the individual components. The surface activity of some of them provides, in addition to the antimicrobial effect, also a cleaning effect and a very strong residual antimicrobial effect remains even after the isopropyl alcohol has evaporated. Due to the presence of isopropyl alcohol, the preparation has a very rapid onset of action (according to microbiological analyzes, the onset of action is almost immediate). The preparation also acts on those microorganisms to which the aqueous solutions of the individual components are not effective.

DETAILED DESCRIPTION OF THE INVENTION

Aqueous solutions of surface-active alkyldimethylamine oxide, e.g. 10 wt. (1-methyldodecyl) dimethylamine oxide, alkyltrimethylammonium bromide, e.g. 10 wt. % carbetopendecin bromide and 20 wt. chlorhexidine di-gluconate is mixed in the necessary volume with pure isopropyl alcohol. The concentration of the resulting solution is adjusted to a final 70% by weight with distilled water. content of isopropyl alcohol. Glycerol whose final concentration in the solution does not exceed 1% by volume is added.

The antimicrobial activity of both the individual components and the resulting product was tested on Cs microorganisms. the State Collection of Type Cultures in Prague, as well as on microorganisms isolated from health facilities. Standard methods used to test disinfectants were used, from microorganisms to Gram-positive and Gram-negative bacteria and microscopic fungi. These microorganisms are effective even in high dilutions within 1 minute. The presence of proteins does not significantly affect efficacy, as fluctuations in temperature of 10 ° C over normal temperature do not affect the antimicrobial activity of the preparation.

To illustrate the efficacy, the results of the laboratory tests are shown in the tables.

I CH ₃ wherein R is a straight or branched alkyl chain having a carbon number from 10 to 18, further 0.5 to 1.0% by weight. an organic ammonium salt of the formula R ₁ R ₂ (CH ₃ ) ₂ N ⁺ X and the structure

Tab. 1

Minimum inhibitory concentrations (MIC) of the preparation in pg.crri ³

Staphylococcus aureus 195.3 Bacillus sublilis 195.3 Escherichia coli 781.2 Salmonella typhimurium 1562.5 Pseudomonas aeruginosa 3125 Proteus vulgaris 6250 Candida albicans 97.6

Table 2

Antimicrobial activity in suspension test

- maximum dilution killing micro-organisms per minute in% (v / v)

Staphylococcus aureus and X Bacillus subtilis 0.3 Escherichia col 0.2 ' Salmonella typhimurium of 2 Pseudomonas aeruginosa 0.3 Proteus vulgaris 0.3 Candida albicans 0.2

In the presence of 20% protein, the efficacy of the preparation is 5 '. aureus does not change in this test, on E. coli the maximum effective dilution increases to 0.3%

Tab. 3

Concentration of preparation required to kill micro-organisms dried on carriers in% (v / v)

time [min] 5 1 3 Staphylococcus aureus 0.06 0.05 0.05 Escherichia coli 1.0 1.0 0.3 Candida albicans 1.0 0.5 0.3

PATENT CLAIMS

Claims (2)

PATENT CLAIMS An isopropanol-containing amine oxide disinfectant, characterized in that it contains 0.1 to 1 wt. alkyldimethylamine oxide of the general formula R (CH ₃ ) ₂ N ⁺ O 'and structures CH ₃ I CH ₃ wherein R is a straight or branched alkyl chain having a carbon number from 10 to 18, further 0.5 to 1% by weight. a dialkyldimethylammonium salt of formula RR2 (CH3); Rf X wherein R 1 represents a linear or branched substituted or unsubstituted alkyl chain having a carbon number of 12 to 16 and R ₂ represents either a methyl or benzyl radical and X 'represents a halide anion and 0.1 to 1 wt. chlorhexidine in the form of digluconate dissolved in 30 to 95% aqueous isopropyl alcohol solution. Isopropyl alcohol-containing amine oxide disinfectants according to claim 1, characterized in that they contain 0.3 wt. % alkyldimethylamine N-oxide having a number of carbon atoms in the alkyl chain of 14; % dialkyldimethylammonium salt with equal number of carbons in both alkyl chains and bromide anion and finally 0.4 wt. chlorhexidine digluconate in 70% aqueous isopropyl alcohol.