RU2297828C2 - Disinfecting agent - Google Patents

Abstract

FIELD: veterinary, medicine, controlling of fungal and bacterial infections.

SUBSTANCE: claimed agent contains alkyldimethylbenzylammonium chloride, mono- or disubstituted potassium, sodium and ammonium salts of phosphoric or carbonic acid or mixture thereof, and water in specific component ratio (mass %).

EFFECT: agent of increased disinfecting activity and decreased active ingredient content.

36 tbl, 37 ex

Description

The invention relates to veterinary medicine and medicine, in particular disinfectants, and can find application in veterinary and medical practice for the disinfection of livestock buildings, poultry houses, dairy and meat processing enterprises and other objects of veterinary surveillance, as well as for surface treatment of premises, sanitary equipment patient care items in medical institutions for bacterial and fungal infections.

It is known to use quaternary ammonium compounds (HOURs) as part of preparations with antiseptic, disinfectant and detergent effects, used in veterinary practice and medicine.

It is known the use of the drug "Glutex" (Glutex) for the prophylactic disinfection of livestock, poultry and animal husbandry premises, equipment, as well as forced disinfection in diseases whose pathogens belong to groups 1 and 2 in terms of resistance to chemical disinfectants. [Veterinary drugs in Russia. Directory. I.F. Klenova, N.A. Yaremenko. M., Selkhozizdat, 2000]. The preparation contains in one liter of a solution 40 g of glutaraldehyde, 32 g of glyoxal, 100 g of didecyldimethylammonium chloride (HOUR). For preventive disinfection, the drug is diluted with water 200 times, and for forced disinfection - 100 times with an exposure of 3 hours. The calculated bactericidal concentration of the components in the working solution will be, wt.%:

- with prophylactic disinfection: glutaraldehyde - 0.020; glyoxal - 0.016; HOUR - 0.050;

- in case of forced disinfection: glutaraldehyde - 0.040; glyoxal - 0.032; HOUR - 0.100.

The total bactericidal concentration of active substances in the working solution is, wt.%:

- with preventive disinfection - 0.086;

- with forced disinfection - 0.172.

The main disadvantage of the known drug is its multicomponent, deficiency and high cost of its constituent components.

A disinfectant for disinfecting patient care items is known. [Patent RU No. 2203094, IPC ⁷ A61L 2/16, A61L 2/18; 2003.04.27. Disinfectant]. The tool contains glutaraldehyde, alkyldimethylbenzylammonium chloride or didecyldimethylammonium chloride (HOUR), 1,4-butanediol, nonionic surfactant (SAS), perfume and water in the following ratio of ingredients, wt. %: glutaraldehyde - 1.4-1.8; HOUR - 1.6-2.0; 1,4-butanediol - 1.6-2.2; nonionic surfactant - 0.4-0.8; perfume - 0.01-0.05; water is the rest. For surface treatment of premises, sanitary equipment, patient care items for bacterial and fungal infections (except for tuberculosis), the drug is diluted 50-100 times with water at a disinfection (exposure) time of 60 minutes. The calculated bactericidal concentration of active substances in the working solution will be, wt. %: glutaraldehyde - 0.014-0.036; butanediol - 0.016-0.040; HOUR - 0.016-0.040. The total bactericidal concentration of active substances in the working solution is 0.046-0.116 wt.%. The known technical solution in its effectiveness does not exceed the previously considered. The main disadvantage of this disinfectant is its multicomponent nature, scarcity and high cost of its constituent components.

A disinfectant is known for use in veterinary practice. [Patent RU No. 2158140, IPC ⁷ A61L 2/16; 2000.10.27. Disinfectant and method for its preparation].

The tool includes glutaraldehyde, water and a Quaternary ammonium compound (QAC) - N- [alkylphenoxypoly (ethyleneoxy) carbonylmethyl] ammonium chloride in the following ratio, wt. %: glutaraldehyde - 20.0-26.0; HOUR - 4.0-20.0; water is the rest.

A disinfectant is obtained by heating a mixture of an aqueous solution of glutaraldehyde and QAC taken in a predetermined ratio for 2-3 hours at a temperature of 50-80 ° C. The bactericidal activity of the agent was determined by the effect of solutions of various concentrations in physiological saline on cultures of Staphylococcus aureus for 2 hours and Mycobacterium bovis for 0.5-2 hours. The minimum bactericidal concentration of the main components in the working solution is, wt. %:

- in relation to Staphylococcus aureus with an exposure of 1 hour: glutaraldehyde - 0.002-0.003; HOUR - 0.0002-0.001. The total bactericidal concentration of active substances in the working solution is 0.0022-0.004%;

- in relation to Mycobacterium bovis at an exposure of 1 hour: glutaraldehyde - 0.1-0.2; HOUR - 0.02-0.2. The total bactericidal concentration of active substances in the working solution is 0.12-0.40%. The main disadvantage of this solution is the scarcity of raw materials - a quaternary ammonium compound - and the need for prolonged heating, which leads to additional costs of thermal energy and financial resources.

Known disinfectant for disinfection of premises, sanitary equipment, items for patient care. [Patent RU No. 2145238, IPC ⁷ A61L 2/16, A61L 2/18; 02/02/10. Disinfectant]. The tool includes glutaraldehyde, glyoxal, nonionic surfactant, alkyldimethylbenzylammonium chloride (HOUR), Trilon B, ethyl alcohol and water in the following ratio of ingredients, wt. %: glutaraldehyde - 0.3-0.7; glyoxal - 3.5-6.5; nonionic surfactant - 4.0-8.0; Alkyldimethylbenzylammonium chloride (HOUR) - 25.0-35.0; Trilon B - 0.08-0.12; ethyl alcohol - 6.0-10.0; water is the rest.

For disinfection with bacterial and viral infections of the surface in the premises, sanitary equipment use 0.1-0.5% solution of the product. The minimum bactericidal concentration of the main components in the working solution is, wt. %: glutaraldehyde - 0.0015-0.007; glyoxal - 0.006-0.017; Alkyldimethylbenzylammonium chloride (HOUR) - 0.035-0.125. The total bactericidal concentration of active substances in the working solution is 0.04-0.15%.

To disinfect various objects with tuberculosis and infections of fungal etiology, a 1% solution of the agent is used. The minimum bactericidal concentration of the main components in the working solution is, wt. %: glutaraldehyde - 0.003-0.007; glyoxal - 0.035-0.065; Alkyldimethylbenzylammonium chloride (HOUR) 0.25-0.35. The total bactericidal concentration of active substances in the working solution is 0.3-0.4%. The well-known technical solution in its effectiveness does not exceed those considered previously. The main disadvantage of this disinfectant is its multicomponent nature.

The closest in technical essence and the achieved result to the claimed invention is a disinfectant with a detergent effect "Catamine AB", used in veterinary medicine at the enterprises of meat and dairy industry for disinfection of the surface of equipment, industrial premises. [one. Instructions for the use of disinfectant with detergent effect "Catamine AB" for the sanitary processing of equipment at the enterprises of the meat industry. Developed by RAAS and VNIIMP. Approved GOS of the Ministry of Health of Russia February 16, 1999, No. 1100 / 357-99-113; 2. Instructions for the use of Kathamine AB disinfectant for the disinfection of equipment and surfaces of industrial premises in the dairy industry. Developed by RAAS and VNIIMP. Approved GSN Ministry of Health of Russia February 16, 1999, No. 1100 / 360-99-113].

To process disinfection objects at meat enterprises, a content of 1.5% alkyldimethylbenzylammonium chloride in the working solution is necessary. To process disinfection objects at dairy enterprises, a content of 0.15-0.25% of alkyldimethylbenzylammonium chloride in the working solution is necessary.

The objective of the present invention is to provide an effective, affordable and cheap disinfectant with antimicrobial activity against gram-negative, gram-positive bacteria and spore cultures, with a minimum amount of active substance in its composition.

To solve this problem, a disinfectant is proposed containing alkyl dimethylbenzylammonium chloride, phosphate or carbonate salt of sodium or potassium, or ammonium, or a mixture thereof and water in the following ratio of components, wt. %:

Alkyldimethylbenzylammonium chloride 0.001-1.5 sodium phosphate or carbonate or potassium, or ammonium, or a mixture thereof 0,0003-6.0 water - the rest.

Preferably, the weight ratio of alkyldimethylbenzylammonium chloride (HAC) to the salt or mixture of salts is from 90:10 to 40:60.

The technical result of the present invention is to increase the effectiveness of the disinfectant while reducing the content of the active substance in its composition.

Only a combination of alkyldimethylbenzylammonium chloride and sodium, potassium or ammonium salts or a mixture thereof in a certain ratio allows us to solve the problem and obtain the specified technical result.

When the content of the components in the composition of the disinfectant is below the minimum limits and the salt or salt mixture content is less than 10% with respect to HOUR, the effectiveness of the disinfectant practically does not differ from the effectiveness of the Catamine AB solution. The content of the components in the composition of the disinfectant is higher than the maximum limits and the content of the salt or salt mixture of more than 60% with respect to HOUR does not increase the disinfecting ability of the disinfectant.

No technical solutions similar to the claimed are known from the patent and scientific and technical literature, which allows us to conclude that the proposal meets the patentability condition - “novelty”.

To prepare the disinfectant solution, the industrial preparation “Catamine AB” is used according to TU 2482-012-13164401, which is a 50% aqueous solution of alkyldimethylbenzylammonium chloride, drinking water according to GOST 2874 and the following salts or their mixture:

- potassium carbonate in accordance with GOST 10690 or GOST 4221;

- sour potassium carbonate in accordance with GOST 4143;

- potassium phosphate monosubstituted according to GOST 4198;

- potassium phosphate disubstituted in accordance with GOST 2493;

- sodium carbonate in accordance with GOST 83;

- sodium bicarbonate in accordance with GOST 2151;

- sodium phosphate monosubstituted in accordance with GOST 245;

- sodium phosphate disubstituted in accordance with GOST 4172;

- ammonium carbonate in accordance with GOST 3770;

- carbonic acid ammonium in accordance with GOST 3762;

- diammonium phosphate according to GOST 8515;

- ammophos according to TU 2387-026-00494172-01;

- ammofoska according to TU 113-08-569-98.

Antimicrobial activity is determined in accordance with the methods and recommendations of the "Instructions for the determination of the bactericidal properties of new disinfectants" No. 739-68 of the USSR Ministry of Health. The invention is illustrated by the following examples.

Example 1

Take 0.2 g of Catamine AB (0.1 g of alkyldimethylbenzylammonium chloride (HOUR) in terms of dry matter), adjusted to 200 g with water. Get a solution with a concentration of alkyldimethylbenzylammonium chloride of 0.05%. 40 g were taken from the resulting solution and adjusted to 100 g with water. Get a solution with a concentration of alkyldimethylbenzylammonium chloride of 0.02%. In a similar manner, a series of solutions are prepared by dilution with a concentration of 0.01% dimethylbenzylammonium chloride; 0.008%; 0.005%; 0.004%. According to the above methodology, the minimum bactericidal concentration of alkyldimethylbenzylammonium chloride is determined using Escherichia coli as the most stable type of intestinal group as a test culture of Escherichia coli. After a certain exposure, the presence or absence of growth of the test culture is determined. The results of testing a series of solutions are presented in the table.

No. p / p The concentration of HOUR, wt. % Exposure time, min fifteen thirty 60 one 0.05 - - - 2 0.02 - - - 3 0.01 + - - four 0.008 + + + 5 0.005 + + + 6 0.004 + + + Note: + - the presence of growth of the test culture;
- lack of growth of the test culture.

Example 2

A sample of 1.0 g of sodium bicarbonate is taken and dissolved in water, bringing the volume of the solution to 100 g. A solution is obtained in 1 g of which contains 10 mg of sodium bicarbonate (solution 1).

Take 0.2 g of Catamine AB (0.1 g of alkyldimethylbenzylammonium chloride, calculated on the dry matter), adjusted to 200 g with water. Get a solution with a concentration of alkyldimethylbenzylammonium chloride (HOUR) of 0.05% (solution 2).

Take 92 g of solution 2, add 0.4 g of solution 1 to it. A solution with a HOUR concentration of 0.05% and a HOUR: salt ratio of 92: 8 is obtained. 40 g were taken from the resulting solution and adjusted to 100 g with water. Get a solution of a disinfectant with a concentration of alkyldimethylbenzylammonium chloride of 0.02%. Similarly, by dilution, a series of disinfectant solutions is prepared with a concentration of alkyldimethylbenzylammonium chloride of 0.01%; 0.008%; 0.005%; 0.004% and with a sodium bicarbonate concentration of 0.00087%, 0.0007%, 0.00043%, 0.00035%, respectively (HR: salt ratio as 92: 8). According to the above methodology, the minimum bactericidal concentration of alkyl dimethylbenzylammonium chloride in a disinfectant solution is determined using Escherichia coli as a test culture of Escherichia coli.

No. p / p The concentration of HOUR in a solution of a disinfectant, wt. % Exposure time, min fifteen thirty 60 one 0.05 - - - 2 0.02 - - - 3 0.01 - - four 0.008 + + - 5 0.005 + + + 6 0.004 + + + Note: + the presence of growth of the test culture; - lack of growth of the test culture

Example 3

A sample of 1.0 g of sodium bicarbonate is taken and dissolved in water, bringing the volume of the solution to 100 g. A solution is obtained in 1 g of which contains 10 mg of sodium bicarbonate (solution 1).

Take 0.2 g of Catamine AB (0.1 g of alkyldimethylbenzylammonium chloride, calculated on the dry matter), adjusted to 200 g with water. Get a solution with a concentration of alkyldimethylbenzylammonium chloride (HOUR) of 0.05% (solution 2).

Take 90 g of solution 2, add 0.5 g of solution 1 to it. A disinfectant solution is obtained with a HOUR concentration of 0.05% and a HOUR: salt ratio of 90:10. 40 g were taken from the resulting solution and adjusted to 100 g with water. Get a solution of a disinfectant with a concentration of alkyldimethylbenzylammonium chloride of 0.02%. Similarly, by dilution, a series of disinfectant solutions is prepared with a concentration of alkyldimethylbenzylammonium chloride of 0.01%; 0.008%; 0.004%; 0.003%, 0.0025% and with a concentration of sodium bicarbonate 0.0011%, 0.00089%, 0.00044%, 0.00033%, 0.00028 - respectively (HR: salt ratio as 90:10). According to the above methodology, the minimum bactericidal concentration of alkyl dimethylbenzylammonium chloride in a disinfectant solution is determined using Escherichia coli as a test culture of Escherichia coli.

No. p / p The concentration of HOUR in a solution of a disinfectant, wt. % Exposure time, min fifteen thirty 60 one 0.05 - - - 2 0.02 - - - 3 0.01 - - - four 0.008 - - - 5 0.004 - - - 6 0.003 - - - 7 0.0025 + - - Note: + the presence of growth of the test culture;
- lack of growth of the test culture

Example 4

Take a sample of 4.0 g of sodium bicarbonate and dissolve in water, bring the volume of the solution to 100 g. Get a solution in 1 g of which contains 40 mg of sodium bicarbonate (solution 1).

Take 0.2 g of Catamine AB (0.1 g of alkyldimethylbenzylammonium chloride, calculated on the dry matter), adjusted to 200 g with water. Get a solution with a concentration of alkyldimethylbenzylammonium chloride (HOUR) of 0.05% (solution 2).

60 g of solution 2 are taken, 0.5 g of solution 1 is added to it. A disinfectant solution is obtained with a HOUR concentration of 0.05% and a HOUR: salt ratio of 60:40. 20 g are taken from the resulting solution and adjusted to 100 g with water. Get a solution of a disinfectant with a concentration of alkyldimethylbenzylammonium chloride of 0.01%. Similarly, by dilution, a series of disinfectant solutions is prepared with a concentration of alkyldimethylbenzylammonium chloride of 0.01%; 0.005%; 0.0025%; 0.0020% and with a concentration of sodium bicarbonate, respectively, 0.066%, 0.0033%, 0.0017%, 0.0013% (HR: salt ratio as 60:40). According to the above methodology, the minimum bactericidal concentration of alkyl dimethylbenzylammonium chloride in a disinfectant solution is determined using Escherichia coli as a test culture of Escherichia coli.

No. p / p The concentration of HOUR in a solution of a disinfectant, wt. % Exposure time, min fifteen thirty 60 one 0.05 - - - 2 0.01 - - - 3 0.005 - - - four 0.0025 - - - 5 0.0020 + - - Note: + the presence of growth of the test culture;
- lack of growth of the test culture

Example 5

Take a sample of 4.0 g of sodium bicarbonate and dissolve in water, bringing the volume of the solution to 100 g. Get a solution in 1 g of which contains 40 mg of sodium bicarbonate (solution 1).

Take 0.2 g of Catamine AB (0.1 g of alkyldimethylbenzylammonium chloride, calculated on the dry matter), adjusted to 200 g with water. Get a solution with a concentration of alkyldimethylbenzylammonium chloride (HOUR) of 0.05% (solution 2).

40 g of solution 2 is taken, 0.75 g of solution 1 is added to it. A disinfectant solution is obtained with a HOUR concentration of 0.05% and an HOUR: salt ratio of 40:60. 20 g are taken from the resulting solution and adjusted to 100 g with water. Get a solution of a disinfectant with a concentration of alkyldimethylbenzylammonium chloride of 0.01%. Similarly, by dilution, a series of disinfectant solutions is prepared with a concentration of alkyldimethylbenzylammonium chloride of 0.01%; 0.005%; 0.0025%; 0.0020% with a concentration of sodium bicarbonate, respectively 0.015%, 0.0075%, 0.00375%, 0.003% (HR: salt ratio as 40:60). According to the above methodology, the minimum bactericidal concentration of alkyl dimethylbenzylammonium chloride in a disinfectant solution is determined using Escherichia coli as a test culture of Escherichia coli.

No. p / p The concentration of HOUR in a solution of a disinfectant, wt. % Exposure time, min fifteen thirty 60 one 0.01 - - - 2 0.005 - - - 3 0.0025 - - - four 0.0020 + - - Note: + the presence of growth of the test culture;
- lack of growth of the test culture

Example 6

Take a sample of 4.0 g of sodium bicarbonate and dissolve in water, bringing the volume of the solution to 100 g. Get a solution in 1 g of which contains 40 mg of sodium bicarbonate (solution 1).

Take 0.2 g of Catamine AB (0.1 g of alkyldimethylbenzylammonium chloride, calculated on the dry matter), adjusted to 200 g with water. Get a solution with a concentration of alkyldimethylbenzylammonium chloride (HOUR) of 0.05% (solution 2).

60 g of solution 2 are taken, 1.75 g of solution 1 is added to it. A disinfectant solution is obtained with a HOUR concentration of 0.05% and an HOUR: salt ratio of 70:30. 20 g are taken from the resulting solution and adjusted to 100 g with water. Get a solution of a disinfectant with a concentration of alkyldimethylbenzylammonium chloride of 0.01%. Similarly, by dilution, a series of disinfectant solutions is prepared with a concentration of alkyldimethylbenzylammonium chloride of 0.01%; 0.005%; 0.0025%; 0.0020% and with a concentration of sodium bicarbonate, respectively, 0.0043%, 0.0021%, 0.0011%, 0.00085% (HR: salt ratio as 70:30). According to the above methodology, the minimum bactericidal concentration of alkyl dimethylbenzylammonium chloride in a disinfectant solution is determined using Escherichia coli as a test culture of Escherichia coli. After a certain exposure, the presence or absence of growth of the test culture was determined. The results of testing a series of solutions are presented in the table.

No. p / p The concentration of HOUR in a solution of a disinfectant, wt. % Exposure time, min fifteen thirty 60 one 0.05 - - 2 0.01 - - - 3 0.005 - - - four 0.0025 - - - 5 0.0020 + - - Note: + the presence of growth of the test culture; - lack of growth of the test culture

Example 7

A series of disinfectant solutions is prepared in accordance with Example 3, using sodium carbonate with a concentration of alkyldimethylbenzylammonium chloride 0.01% as a salt; 0.008%; 0.004%; 0.003%; 0.0025% and with a concentration of sodium carbonate, respectively 0.0011%, 0.00089%, 0.00044%, 0.00033%, 0.00028% (HR: salt ratio as 90:10). According to the above methodology, the minimum bactericidal concentration of alkyl dimethylbenzylammonium chloride in a disinfectant solution is determined using Escherichia coli as a test culture of Escherichia coli.

No. p / p The concentration of HOUR in a solution of a disinfectant, wt. % Exposure time, min fifteen thirty 60 one 0.05 - - - 2 0.02 - - - 3 0.01 - - - four 0.008 - - - 5 0.004 - - - 6 0.003 - - - 7 0.0025 + + - Note: + the presence of growth of the test culture;
- lack of growth of the test culture

Example 8

A series of disinfectant solutions is prepared in accordance with Example 5, using sodium carbonate as a salt with a concentration of alkyldimethylbenzylammonium chloride of 0.01%; 0.005%; 0.0025%; 0.0020% and with a concentration of sodium carbonate, respectively 0.015%, 0.0075%, 0.00375%, 0.003% (HR: salt ratio as 40:60). According to the above methodology, the minimum bactericidal concentration of alkyl dimethylbenzylammonium chloride in a disinfectant solution is determined using Escherichia coli as a test culture of Escherichia coli.

No. p / p The concentration of HOUR in a solution of a disinfectant, wt. % Exposure time, min fifteen thirty 60 one 0.01 - - - 2 0.005 - - - 3 0.0025 - - - four 0.0020 + + - Note: + the presence of growth of the test culture;
- lack of growth of the test culture

Example 9

A series of disinfectant solutions is prepared in accordance with Example 3, using monosubstituted sodium phosphate as a salt with an alkyl dimethyl benzyl ammonium chloride concentration of 0.01%; 0.005%; 0.0025%; 0.0020%; 0.0015% and with a concentration of sodium phosphate monosubstituted 0.0011%, 0.00089%, 0.00044%, 0.00033%, 0.00028 -respectively (HOUR: salt ratio as 90:10). Using the above method, determine the minimum bactericidal concentration of alkyl dimethylbenzylammonium chloride in a disinfectant solution, using Escherichia coli as a test culture of Escherichia coli.

No. p / p The concentration of HOUR in a solution of a disinfectant, wt. % Exposure time, min fifteen thirty 60 one 0.01 - - - 2 0.005 - - - 3 0.0025 - - - four 0.0020 - - - 5 0.0015 + - - Note: + the presence of growth of the test culture;
- lack of growth of the test culture

Example 10

A series of disinfectant solutions is prepared in accordance with Example 5, using monosubstituted sodium phosphate as a salt with a concentration of alkyldimethylbenzylammonium chloride of 0.01%; 0.005%; 0.0025%; 0.0020%; 0.0015% and with a concentration of sodium phosphate monosubstituted, respectively, 0.015%, 0.0075%, 0.00375%, 0.003% (HR: salt ratio as 40:60). According to the above methodology, the minimum bactericidal concentration of alkyl dimethylbenzylammonium chloride in a disinfectant solution is determined using Escherichia coli as a test culture of Escherichia coli.

No. p / p The concentration of HOUR in a solution of a disinfectant, wt. % Exposure time, min fifteen thirty 60 one 0.01 - - - 2 0.005 - - - 3 0.0025 - - - four 0.0020 - - - 5 0.0015 + - - Note: + the presence of growth of the test culture;
- lack of growth of the test culture

Example 11

A series of disinfectant solutions is prepared in accordance with Example 3, using sodium disubstituted phosphate with a concentration of 0.01% dimethylbenzylammonium chloride as a salt; 0.005%; 0.0025%; 0.0020%; 0.0015% and with a concentration of sodium phosphate monosubstituted 0.0011%, 0.00056%, 0.00028%, 0.00022%, 0.00017%, respectively (HR: salt ratio as 90:10). According to the above methodology, the minimum bactericidal concentration of alkyl dimethylbenzylammonium chloride in a disinfectant solution is determined using Escherichia coli as a test culture of Escherichia coli.

No. p / p The concentration of HOUR in a solution of a disinfectant, wt. % Exposure time, min fifteen thirty 60 one 0.01 - - - 2 0.005 - - 3 0.0025 - - - four 0.0020 - - - 5 0.0015 + + Note: + the presence of growth of the test culture;
- lack of growth of the test culture

Example 12

A series of disinfectant solutions is prepared in accordance with Example 5, using disodium disodium phosphate as a salt with a concentration of 0.01% dimethylbenzylammonium chloride; 0.005%; 0.0025%; 0.0020%; 0.0015% and with a concentration of sodium phosphate disubstituted, respectively, 0.015%, 0.0075%, 0.00375%, 0.003%, 0.0023% (HR: salt ratio as 40:60). According to the above methodology, the minimum bactericidal concentration of alkyl dimethylbenzylammonium chloride in a disinfectant solution is determined using Escherichia coli as a test culture of Escherichia coli.

No. p / p The concentration of HOUR in a solution of a disinfectant, wt. % Exposure time, min fifteen thirty 60 one 0.01 - - - 2 0.005 - - - 3 0.0025 - - - four 0.0020 - - - 5 0.0015 + - - Note: + the presence of growth of the test culture;
- lack of growth of the test culture

Example 13

A series of disinfectant solutions is prepared in accordance with Example 3, using potassium carbonate as a salt with a concentration of alkyldimethylbenzylammonium chloride of 0.01%; 0.005%; 0.0025%; 0.0020% and with a concentration of potassium carbonate 0.0011%, 0.00056%, 0.00028%, 0.00022%, respectively (HR: salt ratio as 90:10). According to the above methodology, the minimum bactericidal concentration of alkyl dimethylbenzylammonium chloride in a disinfectant solution is determined using Escherichia coli as a test culture of Escherichia coli.

No. p / p The concentration of HOUR in a solution of a disinfectant, wt. % Exposure time, min fifteen thirty 60 one 0.01 - - - 2 0.005 - - - 3 0.0025 + - - four 0.0020 + + + Note: + the presence of growth of the test culture;
- lack of growth of the test culture

Example 14

A series of disinfectant solutions is prepared in accordance with Example 5, using potassium carbonate as a salt with a concentration of alkyldimethylbenzylammonium chloride of 0.01%; 0.005%; 0.0025%; 0.0020% and with a concentration of potassium carbonate, respectively 0.015%, 0.0075%, 0.00375%, 0.003%, 0.0023% (HR: salt ratio as 40:60). According to the above methodology, the minimum bactericidal concentration of alkyl dimethylbenzylammonium chloride in a disinfectant solution is determined using Escherichia coli as a test culture of Escherichia coli.

No. p / p The concentration of HOUR in a solution of a disinfectant, wt. % Exposure time, min fifteen thirty 60 one 0.01 - - - 2 0.005 - - - 3 0.0025 - - - four 0.0020 - - - Note: + the presence of growth of the test culture;
- lack of growth of the test culture

Example 15

A series of disinfectant solutions is prepared in accordance with Example 3, using acidic potassium carbonate with a concentration of alkyldimethylbenzylammonium chloride 0.01% as a salt; 0.005%; 0.0025%; 0.0020% and with a potassium carbonate concentration of 0.0011%, 0.00056%, 0.00028%, 0.00022%, respectively (HR: salt ratio as 90:10). According to the above methodology, the minimum bactericidal concentration of alkyl dimethylbenzylammonium chloride in a disinfectant solution is determined using Escherichia coli as a test culture of Escherichia coli.

No. p / p The concentration of HOUR in a solution of a disinfectant, wt. % Exposure time, min fifteen thirty 60 one 0.01 - - - 2 0.005 - - - 3 0.0025 + - - four 0.0020 + + + Note: + the presence of growth of the test culture;
- lack of growth of the test culture

Example 16

A series of disinfectant solutions is prepared according to Example 5, using acidic potassium carbonate with a concentration of alkyldimethylbenzylammonium chloride 0.01% as a salt; 0.005%; 0.0025%; 0.0020% and with a concentration of potassium carbonate, respectively 0.015%, 0.0075%, 0.00375%, 0.003%, 0.0023% (HR: salt ratio as 40:60). According to the above methodology, the minimum bactericidal concentration of alkyl dimethylbenzylammonium chloride in a disinfectant solution is determined using Escherichia coli as a test culture of Escherichia coli.

No. p / p The concentration of HOUR in a solution of a disinfectant, wt. % Exposure time, min fifteen thirty 60 one 0.01 - - - 2 0.005 - - - 3 0.0025 + - - four 0.0020 + + + Note: + the presence of growth of the test culture;
- lack of growth of the test culture

Example 17

A series of disinfectant solutions is prepared in accordance with Example 3, using potassium phosphate monosubstituted with an alkyl dimethyl-benzylammonium chloride concentration of 0.01% as a salt; 0.005%; 0.0025%; 0.0020% and with a concentration of potassium phosphate monosubstituted 0.0011%, 0.00056%, 0.00028%, 0.00022%, respectively (HR: salt ratio as 90:10). According to the above methodology, the minimum bactericidal concentration of alkyl dimethylbenzylammonium chloride in a disinfectant solution is determined using Escherichia coli as a test culture of Escherichia coli.

No. p / p The concentration of HOUR in a solution of a disinfectant, wt. % Exposure time, min fifteen thirty 60 one 0.01 - - - 2 0.005 - - - 3 0.0025 - - - four 0.0020 + + - Note: + the presence of growth of the test culture;
- lack of growth of the test culture

Example 18

A series of disinfectant solutions is prepared in accordance with Example 5, using potassium phosphate monosubstituted with an alkyl dimethylbenzylammonium chloride concentration of 0.01% as a salt; 0.005%; 0.0025%; 0.0020% and with a concentration of potassium phosphate monosubstituted, respectively, 0.015%, 0.0075%, 0.00375%, 0.003%, 0.0023% (HR: salt ratio as 40:60). According to the above methodology, the minimum bactericidal concentration of alkyl dimethylbenzylammonium chloride in a disinfectant solution is determined using Escherichia coli as a test culture of Escherichia coli.

No. p / p The concentration of HOUR in a solution of a disinfectant, wt. % Exposure time, min fifteen thirty 60 one 0.01 - - - 2 0.005 - - - 3 0.0025 - - - four 0.0020 + + - Note: + the presence of growth of the test culture;
- lack of growth of the test culture

Example 19

A series of disinfectant solutions is prepared according to Example 3, using potassium phosphate disubstituted with a concentration of alkyl dimethylbenzylammonium chloride 0.01% as a salt; 0.005%; 0.0025%; 0.0020% and with a potassium phosphate disubstituted concentration of 0.0011%, 0.00056%, 0.00028%, 0.00022%, respectively (HR: salt ratio as 90:10). According to the above methodology, the minimum bactericidal concentration of alkyl dimethylbenzylammonium chloride in a disinfectant solution is determined using Escherichia coli as a test culture of Escherichia coli.

No. p / p The concentration of HOUR in a solution of a disinfectant, wt. % Exposure time, min fifteen thirty 60 one 0.01 - - - 2 0.005 - - - 3 0.0025 - - - four 0.0020 + + - Note: + the presence of growth of the test culture;
- lack of growth of the test culture

Example 20

A series of disinfectant solutions is prepared in accordance with Example 5, using potassium carbonate as a salt with a concentration of alkyldimethylbenzylammonium chloride of 0.01%; 0.005%; 0.0025%; 0.0020% and with a concentration of potassium carbonate, respectively 0.015%, 0.0075%, 0.00375%, 0.003%, 0.0023% (HR: salt ratio as 40:60). According to the above methodology, the minimum bactericidal concentration of alkyl dimethylbenzylammonium chloride in a disinfectant solution is determined using Escherichia coli as a test culture of Escherichia coli.

No. p / p The concentration of HOUR in a solution of a disinfectant, wt. % Exposure time, min fifteen thirty 60 one 0.01 - - - 2 0.005 - - - 3 0.0025 - - - four 0.0020 + + - Note: + the presence of growth of the test culture;
- lack of growth of the test culture

Example 21

A series of disinfectant solutions is prepared in accordance with Example 3, using carbon dioxide as an ammonium salt with an alkyl dimethylbenzylammonium chloride concentration of 0.01%; 0.005%; 0.0025%; 0.0020% and with a concentration of ammonium carbonate 0.0011%, 0.00056%, 0.00028%, 0.00022%, respectively (HR: salt ratio as 90:10). According to the above methodology, the minimum bactericidal concentration of alkyl dimethylbenzylammonium chloride in a disinfectant solution is determined using Escherichia coli as a test culture of Escherichia coli.

No. p / p The concentration of HOUR in a solution of a disinfectant, wt. % Exposure time, min fifteen thirty 60 one 0.01 - - - 2 0.005 - - - 3 0.0025 + - - four 0.0020 + + + Note: + the presence of growth of the test culture;
- lack of growth of the test culture

Example 22

A series of disinfectant solutions is prepared in accordance with Example 5, using carbon dioxide as an ammonium salt with a concentration of alkyl dimethyl benzyl ammonium chloride of 0.01%; 0.005%; 0.0025%; 0.0020% and with a concentration of potassium ammonium carbonate, respectively 0.015%, 0.0075%, 0.00375%, 0.003%, 0.0023% (HR: salt ratio as 40:60). According to the above methodology, the minimum bactericidal concentration of alkyl dimethylbenzylammonium chloride in a disinfectant solution is determined using Escherichia coli as a test culture of Escherichia coli.

No. p / p The concentration of HOUR in a solution of a disinfectant, wt. % Exposure time, min fifteen thirty 60 one 0.01 - - - 2 0.005 - - - 3 0.0025 + - - four 0.0020 + + + Note: + the presence of growth of the test culture;
- lack of growth of the test culture

Example 23

A series of disinfectant solutions is prepared in accordance with Example 5, using acidic carbonate as an ammonium salt with a concentration of alkyldimethylbenzylammonium chloride of 0.01%; 0.005%; 0.0025%; 0.0020% and with a concentration of ammonium carbonate, respectively, 0.015%, 0.0075%, 0.00375%, 0.003%, 0.0023% (HR: salt ratio as 40:60). According to the above methodology, the minimum bactericidal concentration of alkyl dimethylbenzylammonium chloride in a disinfectant solution is determined using Escherichia coli as a test culture of Escherichia coli.

No. p / p The concentration of HOUR in a solution of a disinfectant, wt. % Exposure time, min fifteen thirty 60 one 0.01 - - - 2 0.005 - - - 3 0.0025 + - - four 0.0020 + + + Note: + the presence of growth of the test culture;
- lack of growth of the test culture

Example 24

A series of disinfectant solutions is prepared in accordance with Example 3, using diammonium phosphate as a salt with a concentration of alkyl dimethyl benzyl ammonium chloride of 0.01%; 0.005%; 0.0025%; 0.0020% and with a concentration of diammonium phosphate 0.0011%, 0.00056%, 0.00028%, 0.00022%, respectively (HR: salt ratio as 90:10). According to the above methodology, the minimum bactericidal concentration of alkyl dimethylbenzylammonium chloride in a disinfectant solution is determined using Escherichia coli as a test culture of Escherichia coli.

No. p / p The concentration of HOUR in a solution of a disinfectant, wt. % Exposure time, min fifteen thirty 60 one 0.01 - - - 2 0.005 - - - 3 0.0025 - - four 0.0020 + - - Note: + the presence of growth of the test culture;
- lack of growth of the test culture

Example 25

A series of disinfectant solutions is prepared in accordance with Example 5, using diammonium phosphate with a concentration of alkyldimethylbenzylammonium chloride 0.01% as a salt; 0.005%; 0.0025%; 0.0020% and with a concentration of diammonium phosphate, respectively 0.015%, 0.0075%, 0.00375%, 0.003% (HR: salt ratio as 40:60). According to the above methodology, the minimum bactericidal concentration of alkyl dimethylbenzylammonium chloride in a disinfectant solution is determined using Escherichia coli as a test culture of Escherichia coli.

No. p / p The concentration of HOUR in a solution of a disinfectant, wt. % Exposure time, min fifteen thirty 60 one 0.01 - - - 2 0.005 - - - 3 0.0025 - - - four 0.0020 + - - Note: + the presence of growth of the test culture;
- lack of growth of the test culture

Example 26

A series of disinfectant solutions is prepared according to Example 3, using Ammophos as a salt with a concentration of alkyldimethylbenzylammonium chloride of 0.01%; 0.005%; 0.0025%; 0.0020% and with a concentration of Ammophos 0.0011%, 0.00056%, 0.00028%, 0.00022%, respectively (HR: salt ratio as 90:10). According to the above methodology, the minimum bactericidal concentration of alkyl dimethylbenzylammonium chloride in a disinfectant solution is determined using Escherichia coli as a test culture of Escherichia coli.

No. p / p The concentration of HOUR in a solution of a disinfectant, wt. % Exposure time, min fifteen thirty 60 one 0.01 - - - 2 0.005 - - - 3 0.0025 - - four 0.0020 + - - Note: + the presence of growth of the test culture;
- lack of growth of the test culture

Example 27

A series of disinfectant solutions is prepared in accordance with Example 5, using Ammophos as a salt with a concentration of alkyldimethylbenzylammonium chloride of 0.01%; 0.005%; 0.0025%; 0.0020% and with a concentration of Ammophos, respectively 0.015%, 0.0075%, 0.00375%, 0.003%, 0.0023% (HR: salt ratio as 40:60). According to the above methodology, the minimum bactericidal concentration of alkyl dimethylbenzylammonium chloride in a disinfectant solution is determined using Escherichia coli as a test culture of Escherichia coli.

No. p / p The concentration of HOUR in a solution of a disinfectant, wt. % Exposure time, min fifteen thirty 60 one 0.01 - - - 2 0.005 - - - 3 0.0025 - - - four 0.0020 + - - Note: + the presence of growth of the test culture;
- lack of growth of the test culture

Example 28

A series of disinfectant solutions is prepared in accordance with Example 3, using Ammofoska as a salt with a concentration of alkyldimethylbenzylammonium chloride of 0.01%; 0.005%; 0.0025%; 0.0020% and with a concentration of Ammofoski 0.0011%, 0.00056%, 0.00028%, 0.00022%, respectively (HR: salt ratio as 90:10). According to the above methodology, the minimum bactericidal concentration of alkyl dimethylbenzylammonium chloride in a disinfectant solution is determined using Escherichia coli as a test culture of Escherichia coli.

No. p / p The concentration of HOUR in a solution of a disinfectant, wt. % Exposure time, min fifteen thirty 60 one 0.01 - - - 2 0.005 - - - 3 0.0025 - - - four 0.0020 + - - Note: + the presence of growth of the test culture;
- lack of growth of the test culture

Example 29

A series of disinfectant solutions is prepared in accordance with Example 5, using Ammofoska as a salt with a concentration of alkyldimethylbenzylammonium chloride of 0.01%; 0.005%; 0.0025%; 0.0020% and with a concentration of Ammofoski, respectively 0.015%, 0.0075%, 0.00375%, 0.003%, 0.0023% (HR: salt ratio as 40:60). According to the above methodology, the minimum bactericidal concentration of alkyl dimethylbenzylammonium chloride in a disinfectant solution is determined using Escherichia coli as a test culture of Escherichia coli.

No. p / p The concentration of HOUR in a solution of a disinfectant, wt. % Exposure time, min fifteen thirty 60 one 0.01 - - - 2 0.005 - - - 3 0.0025 - - - four 0.0020 + - - Note: + the presence of growth of the test culture;
- lack of growth of the test culture

Example 30

A series of disinfectant solutions is prepared according to Example 3, using as a salt a mixture of 0.5 g sodium bicarbonate and 0.5 g sodium disubstituted phosphate, with a concentration of 0.01% dimethylbenzylammonium chloride; 0.005%; 0.0025%; 0.0020%; 0.0015% and with a concentration of said mixture of salts 0.0011%, 0.00056%, 0.00028%, 0.00022%, respectively (HR: salt ratio as 90:10). According to the above methodology, the minimum bactericidal concentration of alkyl dimethylbenzylammonium chloride in a disinfectant solution is determined using Escherichia coli as a test culture of Escherichia coli.

No. p / p The concentration of HOUR in a solution of a disinfectant, wt. % Exposure time, min fifteen thirty 60 one 0.01 - - - 2 0.005 - - - 3 0.0025 - - four 0.0020 - - - 5 0.0015 + - - Note: + the presence of growth of the test culture;
- lack of growth of the test culture

Example 31

A series of disinfectant solutions is prepared according to Example 5, using as a salt a mixture of 2.0 g of sodium bicarbonate and 2.0 g of sodium phosphate disubstituted, with a concentration of alkyl dimethylbenzylammonium chloride of 0.01%; 0.005%; 0.0025%; 0.0020%; 0.0015% and with a concentration of a mixture of salts, respectively, 0.015%, 0.0075%, 0.00375%, 0.003%, 0.0023% (HR: salt ratio as 40:60). According to the above methodology, the minimum bactericidal concentration of alkyl dimethylbenzylammonium chloride in a disinfectant solution is determined using Escherichia coli as a test culture of Escherichia coli.

No. p / p The concentration of HOUR in a solution of a disinfectant, wt. % Exposure time, min fifteen thirty 60 one 0.01 - - - 2 0.005 - - - 3 0.0025 - - - four 0.0020 - - - 5 0.0015 + - - Note: + the presence of growth of the test culture;
- lack of growth of the test culture

Example 32

A series of disinfectant solutions is prepared according to Example 3, using as a salt a mixture of 0.5 g of sodium bicarbonate and 0.5 g of diammonium phosphate, with a concentration of alkyldimethylbenzylammonium chloride of 0.01%; 0.005%; 0.0025%; 0.0020%; 0.0015% and with a concentration of said mixture of salts 0.0011%, 0.00056%, 0.00028%, 0.00022%, respectively (HR: salt ratio as 90:10). According to the above methodology, the minimum bactericidal concentration of alkyl dimethylbenzylammonium chloride in a disinfectant solution is determined using Escherichia coli as a test culture of Escherichia coli.

No. p / p The concentration of HOUR in a solution of a disinfectant, wt. % Exposure time, min fifteen thirty 60 one 0.01 - - - 2 0.005 - - - 3 0.0025 - - - four 0.0020 - . - - 5 0.0015 + - - Note: + the presence of growth of the test culture;
- lack of growth of the test culture

Example 33

A series of disinfectant solutions is prepared according to Example 5, using as a salt a mixture of 2.0 g of sodium bicarbonate and 2.0 g of diammonium phosphate, with a concentration of alkyldimethylbenzylammonium chloride of 0.01%; 0.005%; 0.0025%; 0.0020%; 0.0015% and with a concentration of said mixture of salts, respectively, 0.015%, 0.0075%, 0.00375%, 0.003%, 0.0023% (HR: salt ratio as 40:60). According to the above methodology, the minimum bactericidal concentration of alkyl dimethylbenzylammonium chloride in a disinfectant solution is determined using Escherichia coli as a test culture of Escherichia coli.

No. p / p The concentration of HOUR in a solution of a disinfectant, wt. % Exposure time, min fifteen thirty 60 one 0.01 - - - 2 0.005 - - - 3 0.0025 - - - four 0.0020 - - - 5 0.0015 + - - Note: + the presence of growth of the test culture;
- lack of growth of the test culture

Example 34

A series of disinfectant solutions is prepared in accordance with Example 5, using sodium bicarbonate as a salt with an alkyl dimethylbenzylammonium chloride concentration of 0.01%; 0.005%; 0.0025%, 0.0020 and with a concentration of sodium bicarbonate, respectively 0.015%, 0.0075%, 0.00375%, 0.003%, 0.0023% (HR: salt ratio as 40:60). According to the above methodology, the minimum bactericidal concentration of alkyl dimethylbenzylammonium chloride in a disinfectant solution is determined using Escherichia coli Escherichia coli, Staphylococcus aureus Staphylococcus aureus, Pseudomonas acruginosa Pseudomonas bacillus, Anthracoid Vas as a test culture. Anthracoides in spore form. After a certain exposure, the presence or absence of growth of the test culture is determined. The results of testing a series of disinfectant solutions are presented in the table.

No. p / p Type of microorganism The concentration of HOUR in a solution of a disinfectant, wt. % Death time, min one Escherichia coli 0.01 fifteen 0.005 fifteen 0.0025 fifteen 0.0020 thirty 2 Staphylococcus aureus 0.01 fifteen 0.005 fifteen 0.0025 thirty 0.0020 thirty 3 Pseudomonas acruginosa 0.01 fifteen 0.005 fifteen 0.0025 thirty 0.0020 thirty four You. Anthracoides 0.01 60 0.005 90 0.0025 120 0.0020 120

Example 35

Prepare a series of solutions of the disinfectant according to example 12, using sodium phosphate disubstituted with a concentration of alkyl dimethylbenzylammonium chloride 0.01% as a salt; 0.005%; 0.0025%, 0.0020; 0.0015% and with a concentration of sodium phosphate disubstituted, respectively, 0.015%, 0.0075%, 0.00375%, 0.003%, 0.0023% (HR: salt ratio as 40:60). According to the above methodology, the minimum bactericidal concentration of alkyl dimethylbenzylammonium chloride in a disinfectant solution is determined using Escherichia coli Escherichia coli, Staphylococcus aureus Staphylococcus aureus, Pseudomonas acruginosa Pseudomonas bacillus, Anthracoid Vas as a test culture. Anthracoides in spore form. After a certain exposure, the presence or absence of growth of the test culture is determined. The results of testing a series of disinfectant solutions are presented in the table.

No. p / p Type of microorganism The concentration of HOUR in a solution of a disinfectant, wt. % Death time, min one 2 3 four one Escherichia coli 0.01 fifteen 0.005 fifteen 0.0025 fifteen 0.0020 fifteen 0.0015 thirty one 2 3 four 2 Staphylococcus aureus 0.01 fifteen 0.005 fifteen 0.0025 fifteen 0.0020 thirty 0.0015 60 3 Pseudomonas acruginosa 0.01 fifteen 0.005 fifteen 0.0025 thirty 0.0020 thirty 0.0015 60 four You. Anthracoides 0.01 60 0.005 60 0.0025 90 0.0020 120 0.0015 120

Example 36

A series of disinfectant solutions is prepared according to Example 33, using as a salt a mixture of 2.0 g of sodium bicarbonate and 2.0 g of diammonium phosphate, with a concentration of alkyldimethylbenzylammonium chloride of 0.01%; 0.005%; 0.0025%, 0.0020; 0.0015% and with a concentration of a mixture of salts, respectively, 0.015%, 0.0075%, 0.00375%, 0.003%, 0.0023% (HR: salt ratio as 40:60). According to the above methodology, the minimum bactericidal concentration of alkyl dimethylbenzylammonium chloride in a disinfectant solution is determined using Escherichia coli Escherichia coli, Staphylococcus aureus Staphylococcus aureus, Pseudomonas acruginosa Pseudomonas bacillus, Anthracoid Vas as a test culture. Anthracoides in spore form. After a certain exposure, the presence or absence of growth of the test culture is determined. The results of testing a series of disinfectant solutions are presented in the table.

No. p / p Type of microorganism The concentration of HOUR in a solution of a disinfectant, wt. % Death time, min one 2 3 four one Escherichia coli 0.01 fifteen 0.005 fifteen 0.0025 fifteen 0.0020 fifteen 0.0015 thirty 2 Staphylococcus aureus 0.01 fifteen 0.005 fifteen 0.0025 fifteen 0.0020 thirty 0.0015 60 one 2 3 four 3 Pseudomonas acruginosa 0.01 fifteen 0.005 fifteen 0.0025 thirty 0.0020 thirty 0.0015 60 four You. Anthracoides 0.01 60 0.005 60 0.0025 90 0.0020 120 0.0015 120

Example 37

The bactericidal activity of disinfectant solutions prepared according to examples 5; 12; 33, was tested on test objects (wooden plates 10 × 10 cm in size, painted with oil paint), onto the surface of which a strain of Escherichia coli was applied at the rate of 0.5 g of a two-billionth suspension per 100 cm ² . The surface of the test objects was dried at room temperature for two hours. They took a series of disinfectant solutions prepared according to examples 5; 12; 33, with a concentration of alkyldimethylbenzylammonium chloride of 0.01%; 0.005%; 0.0025%, 0.0020 and an HOUR: salt ratio of 40:60 and a spray gun irrigated the surface of test objects contaminated with the pathogen. After irrigation, test objects were left to dry completely (50-60 minutes). In parallel, we set up control experiments in which infected surfaces were irrigated with drinking water. The effectiveness of disinfection was monitored by washing off infected surfaces, followed by the introduction of washing liquid into Petri dishes with agar-agar. In all washes from surfaces treated with disinfectant solutions, the growth of the reference culture was not observed.

The data presented in the examples show that the minimum bactericidal concentration of the active substance - HOUR - is only 0.0015-0.0025% against 0.01% in the prototype. Other known disinfectants, close in effectiveness to the claimed, consist of a large number of expensive components and require significant material costs for production.

Thus, the claimed disinfectant consists of only two components manufactured by the industry, surpasses the used disinfectants and prototype in its bactericidal and disinfecting ability and can be used in veterinary and medical practice for disinfection of livestock buildings, poultry houses, dairy and meat processing enterprises and other objects of veterinary surveillance as well as for surface treatment of premises, sanitary equipment, patient care items in pocket medical institutions.

Claims (1)

A disinfectant containing alkyldimethylbenzylammonium chloride and a salt of phosphoric and carbonic acid, characterized in that the salt contains one-, two-substituted potassium, sodium and ammonium salts of phosphoric and carbonic acids or their mixture and water in the following ratio, wt.%: Alkyldimethylbenzylammonium chloride 0.001-1.5 Single, double substituted potassium, sodium or ammonium salts phosphoric or carbonic acids, or a mixture thereof 0,0003-6.0 Water Rest