SG184592A1 - Isolating target cells from a biological fluid - Google Patents
Isolating target cells from a biological fluid Download PDFInfo
- Publication number
- SG184592A1 SG184592A1 SG2011019775A SG2011019775A SG184592A1 SG 184592 A1 SG184592 A1 SG 184592A1 SG 2011019775 A SG2011019775 A SG 2011019775A SG 2011019775 A SG2011019775 A SG 2011019775A SG 184592 A1 SG184592 A1 SG 184592A1
- Authority
- SG
- Singapore
- Prior art keywords
- cell isolation
- micro
- isolation wells
- biological fluid
- fluidic device
- Prior art date
Links
- 239000013060 biological fluid Substances 0.000 title claims abstract description 48
- 238000002955 isolation Methods 0.000 claims abstract description 187
- 238000003491 array Methods 0.000 claims abstract description 99
- 239000002699 waste material Substances 0.000 claims abstract description 47
- 238000000034 method Methods 0.000 claims abstract description 35
- 239000012530 fluid Substances 0.000 claims abstract description 24
- 230000008878 coupling Effects 0.000 claims abstract description 15
- 238000010168 coupling process Methods 0.000 claims abstract description 15
- 238000005859 coupling reaction Methods 0.000 claims abstract description 15
- 238000012423 maintenance Methods 0.000 claims abstract description 15
- 230000004044 response Effects 0.000 claims abstract description 6
- 238000011045 prefiltration Methods 0.000 claims description 77
- 239000003153 chemical reaction reagent Substances 0.000 claims description 39
- 238000011084 recovery Methods 0.000 claims description 23
- 230000008859 change Effects 0.000 claims description 5
- 238000012545 processing Methods 0.000 abstract description 13
- 230000000717 retained effect Effects 0.000 abstract description 7
- 210000004027 cell Anatomy 0.000 description 290
- 239000000523 sample Substances 0.000 description 60
- 206010028980 Neoplasm Diseases 0.000 description 36
- 210000004369 blood Anatomy 0.000 description 32
- 239000008280 blood Substances 0.000 description 32
- 201000011510 cancer Diseases 0.000 description 31
- 210000005266 circulating tumour cell Anatomy 0.000 description 26
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 10
- 201000010099 disease Diseases 0.000 description 9
- 239000000463 material Substances 0.000 description 9
- 230000008569 process Effects 0.000 description 8
- 230000007423 decrease Effects 0.000 description 7
- 238000010186 staining Methods 0.000 description 7
- 239000000470 constituent Substances 0.000 description 6
- 210000000349 chromosome Anatomy 0.000 description 5
- 230000001419 dependent effect Effects 0.000 description 5
- 210000005259 peripheral blood Anatomy 0.000 description 5
- 239000011886 peripheral blood Substances 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- 238000004458 analytical method Methods 0.000 description 4
- 210000000601 blood cell Anatomy 0.000 description 4
- 239000007850 fluorescent dye Substances 0.000 description 4
- 238000007901 in situ hybridization Methods 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 238000012986 modification Methods 0.000 description 4
- 230000004048 modification Effects 0.000 description 4
- 230000002829 reductive effect Effects 0.000 description 4
- 238000013459 approach Methods 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
- 238000011161 development Methods 0.000 description 3
- 230000001605 fetal effect Effects 0.000 description 3
- 238000003125 immunofluorescent labeling Methods 0.000 description 3
- 238000012544 monitoring process Methods 0.000 description 3
- 230000035899 viability Effects 0.000 description 3
- 208000005443 Circulating Neoplastic Cells Diseases 0.000 description 2
- 206010027476 Metastases Diseases 0.000 description 2
- -1 and D.T. Chiu Proteins 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 238000005345 coagulation Methods 0.000 description 2
- 230000015271 coagulation Effects 0.000 description 2
- 230000034994 death Effects 0.000 description 2
- 231100000517 death Toxicity 0.000 description 2
- 238000003745 diagnosis Methods 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 238000011065 in-situ storage Methods 0.000 description 2
- 230000007246 mechanism Effects 0.000 description 2
- 230000009401 metastasis Effects 0.000 description 2
- 230000000704 physical effect Effects 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 230000002441 reversible effect Effects 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 230000003068 static effect Effects 0.000 description 2
- 210000000130 stem cell Anatomy 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- 230000004083 survival effect Effects 0.000 description 2
- 230000007704 transition Effects 0.000 description 2
- 238000011282 treatment Methods 0.000 description 2
- 210000004881 tumor cell Anatomy 0.000 description 2
- 238000012800 visualization Methods 0.000 description 2
- 206010006187 Breast cancer Diseases 0.000 description 1
- 208000026310 Breast neoplasm Diseases 0.000 description 1
- 208000037088 Chromosome Breakage Diseases 0.000 description 1
- 206010009944 Colon cancer Diseases 0.000 description 1
- 108020004414 DNA Proteins 0.000 description 1
- 206010061818 Disease progression Diseases 0.000 description 1
- 102000018651 Epithelial Cell Adhesion Molecule Human genes 0.000 description 1
- 108010066687 Epithelial Cell Adhesion Molecule Proteins 0.000 description 1
- 108020004711 Nucleic Acid Probes Proteins 0.000 description 1
- 108091028043 Nucleic acid sequence Proteins 0.000 description 1
- 241001296119 Panteles Species 0.000 description 1
- 101100017603 Rattus norvegicus Hopx gene Proteins 0.000 description 1
- 108020004511 Recombinant DNA Proteins 0.000 description 1
- 208000007536 Thrombosis Diseases 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 238000002306 biochemical method Methods 0.000 description 1
- 229920000249 biocompatible polymer Polymers 0.000 description 1
- 239000000090 biomarker Substances 0.000 description 1
- 238000001574 biopsy Methods 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 210000000481 breast Anatomy 0.000 description 1
- 230000005773 cancer-related death Effects 0.000 description 1
- 230000006727 cell loss Effects 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 210000001072 colon Anatomy 0.000 description 1
- 208000029742 colonic neoplasm Diseases 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 230000001010 compromised effect Effects 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000004132 cross linking Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 239000004205 dimethyl polysiloxane Substances 0.000 description 1
- 230000005750 disease progression Effects 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 238000012137 double-staining Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000000799 fluorescence microscopy Methods 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 238000007306 functionalization reaction Methods 0.000 description 1
- 230000014509 gene expression Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000003365 immunocytochemistry Methods 0.000 description 1
- 238000010166 immunofluorescence Methods 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 230000002452 interceptive effect Effects 0.000 description 1
- 230000002147 killing effect Effects 0.000 description 1
- 231100000225 lethality Toxicity 0.000 description 1
- 210000000265 leukocyte Anatomy 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 238000007885 magnetic separation Methods 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 108020004999 messenger RNA Proteins 0.000 description 1
- 230000001394 metastastic effect Effects 0.000 description 1
- 206010061289 metastatic neoplasm Diseases 0.000 description 1
- 238000010606 normalization Methods 0.000 description 1
- 239000002853 nucleic acid probe Substances 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 238000011275 oncology therapy Methods 0.000 description 1
- 229920000435 poly(dimethylsiloxane) Polymers 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000000246 remedial effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000007873 sieving Methods 0.000 description 1
- 238000002174 soft lithography Methods 0.000 description 1
- 238000000638 solvent extraction Methods 0.000 description 1
- 239000013077 target material Substances 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 230000003245 working effect Effects 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
- B01L2200/0652—Sorting or classification of particles or molecules
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
- B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
- B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
- B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/08—Regulating or influencing the flow resistance
- B01L2400/084—Passive control of flow resistance
- B01L2400/086—Passive control of flow resistance using baffles or other fixed flow obstructions
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Fluid Mechanics (AREA)
- Dispersion Chemistry (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Hematology (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Priority Applications (6)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SG2011019775A SG184592A1 (en) | 2011-03-18 | 2011-03-18 | Isolating target cells from a biological fluid |
| PCT/SG2012/000084 WO2012128717A1 (en) | 2011-03-18 | 2012-03-13 | Isolating target cells from a biological fluid |
| EP12711474.2A EP2686106A1 (de) | 2011-03-18 | 2012-03-13 | Isolierung von zielzellen aus einer biologischen flüssigkeit |
| SG2013062898A SG192848A1 (en) | 2011-03-18 | 2012-03-13 | Isolating target cells from a biological fluid |
| CN2012800142622A CN103459034A (zh) | 2011-03-18 | 2012-03-13 | 从生物流体中分离目标细胞 |
| US14/030,343 US20140087456A1 (en) | 2009-04-24 | 2013-09-18 | Isolating Target Cells From A Biological Fluid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SG2011019775A SG184592A1 (en) | 2011-03-18 | 2011-03-18 | Isolating target cells from a biological fluid |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SG184592A1 true SG184592A1 (en) | 2012-10-30 |
Family
ID=45922787
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG2011019775A SG184592A1 (en) | 2009-04-24 | 2011-03-18 | Isolating target cells from a biological fluid |
| SG2013062898A SG192848A1 (en) | 2011-03-18 | 2012-03-13 | Isolating target cells from a biological fluid |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG2013062898A SG192848A1 (en) | 2011-03-18 | 2012-03-13 | Isolating target cells from a biological fluid |
Country Status (4)
| Country | Link |
|---|---|
| EP (1) | EP2686106A1 (de) |
| CN (1) | CN103459034A (de) |
| SG (2) | SG184592A1 (de) |
| WO (1) | WO2012128717A1 (de) |
Families Citing this family (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3305918B1 (de) | 2012-03-05 | 2020-06-03 | President and Fellows of Harvard College | Verfahren für epigenetische sequenzierung |
| CN108212237B (zh) * | 2013-03-06 | 2020-12-08 | 哈佛学院院长及董事 | 形成相对单分散液滴的装置和方法 |
| JP6314220B2 (ja) * | 2013-10-16 | 2018-04-18 | クリアブリッジ バイオメディックス ピーティーイー エルティーディーＣｌｅａｒｂｒｉｄｇｅ Ｂｉｏｍｅｄｉｃｓ Ｐｔｅ Ｌｔｄ | 細胞の検出及び単離のためのマイクロ流体選別器 |
| US20150298091A1 (en) | 2014-04-21 | 2015-10-22 | President And Fellows Of Harvard College | Systems and methods for barcoding nucleic acids |
| JP6611223B2 (ja) * | 2015-03-13 | 2019-11-27 | 国立大学法人名古屋大学 | 微粒子分離用チップ、該微粒子分離用チップを用いた微粒子分離用システム、該部粒子分離用システムを用いた微粒子分離方法及び微粒子抽出方法 |
| US11746367B2 (en) | 2015-04-17 | 2023-09-05 | President And Fellows Of Harvard College | Barcoding systems and methods for gene sequencing and other applications |
| CN107400623B (zh) * | 2016-05-20 | 2020-11-24 | 益善生物技术股份有限公司 | 循环肿瘤细胞自动捕获微流控芯片及其自动捕获方法 |
| CN106222088B (zh) * | 2016-09-21 | 2018-06-15 | 东南大学 | 一种用于动物组织原位对照培养的微流控芯片 |
| JP6953561B2 (ja) * | 2017-06-26 | 2021-10-27 | 華為技術有限公司Ｈｕａｗｅｉ Ｔｅｃｈｎｏｌｏｇｉｅｓ Ｃｏ．，Ｌｔｄ． | フィーディングデバイス |
| CN112154031B (zh) * | 2018-04-04 | 2023-05-23 | 综合医院公司 | 用于剥离哺乳动物卵母细胞的微流体系统和方法 |
| EP3883690A1 (de) | 2018-11-21 | 2021-09-29 | BVW Holding AG | Mikrostrukturierte unterscheidungsvorrichtung |
| US20220064627A1 (en) * | 2019-01-23 | 2022-03-03 | Haplomic Technologies Pty Ltd | Microfluidic device |
| EP3714977A1 (de) * | 2019-03-29 | 2020-09-30 | Roche Diagnostics GmbH | Mikrofluidische vorrichtung |
| CN110777048A (zh) * | 2019-11-01 | 2020-02-11 | 上海市第六人民医院 | 一种高通量单外泌体分离装置 |
| CN114939447A (zh) * | 2022-05-22 | 2022-08-26 | 苏州大学 | 集细胞类人体培养及细胞机械特性测量的一体化芯片 |
| CN117110402B (zh) * | 2023-10-23 | 2024-02-27 | 深圳大学 | 一种用于单细胞检测的有机薄膜晶体管片上集成系统 |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6319719B1 (en) * | 1999-10-28 | 2001-11-20 | Roche Diagnostics Corporation | Capillary hematocrit separation structure and method |
| EP1543101A4 (de) * | 2002-08-27 | 2007-06-27 | Univ Vanderbilt | Bioreaktoren mit mehreren kammern |
| US7534601B2 (en) * | 2002-08-27 | 2009-05-19 | Vanderbilt University | Capillary perfused bioreactors with multiple chambers |
| US20050095602A1 (en) * | 2003-11-04 | 2005-05-05 | West Jason A. | Microfluidic integrated microarrays for biological detection |
| DE10352535A1 (de) * | 2003-11-07 | 2005-06-16 | Steag Microparts Gmbh | Mikrostrukturierte Trennvorrichtung und Verfahren zum Abtrennen von flüssigen Bestandteilen aus einer Partikel enthaltenden Flüssigkeit |
| US20100003666A1 (en) * | 2005-08-19 | 2010-01-07 | The Regents Of The University Of California | Microfluidic Methods for Diagnostics and Cellular Analysis |
| CN101548004A (zh) * | 2005-08-19 | 2009-09-30 | 加利福尼亚大学董事会 | 用于诊断学和细胞分析的微流体方法 |
| EP1931800A4 (de) * | 2005-09-15 | 2011-06-15 | Artemis Health Inc | Systeme und verfahren zur anreicherung von analyten |
-
2011
- 2011-03-18 SG SG2011019775A patent/SG184592A1/en unknown
-
2012
- 2012-03-13 EP EP12711474.2A patent/EP2686106A1/de not_active Withdrawn
- 2012-03-13 CN CN2012800142622A patent/CN103459034A/zh active Pending
- 2012-03-13 WO PCT/SG2012/000084 patent/WO2012128717A1/en active Application Filing
- 2012-03-13 SG SG2013062898A patent/SG192848A1/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| WO2012128717A1 (en) | 2012-09-27 |
| EP2686106A1 (de) | 2014-01-22 |
| SG192848A1 (en) | 2013-09-30 |
| CN103459034A (zh) | 2013-12-18 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| SG184592A1 (en) | Isolating target cells from a biological fluid | |
| US20140087456A1 (en) | Isolating Target Cells From A Biological Fluid | |
| DK2440941T3 (en) | Sheath flow devices and methods | |
| US11808767B2 (en) | Methods, compositions and systems for microfluidic assays | |
| US20210356383A1 (en) | Cell capture system and method of use | |
| US11427798B2 (en) | Microfluidic tissue dissociation device and method | |
| Huang et al. | Highly sensitive enumeration of circulating tumor cells in lung cancer patients using a size-based filtration microfluidic chip | |
| US9420148B2 (en) | High-throughput single-cell imaging, sorting, and isolation | |
| Warkiani et al. | An ultra-high-throughput spiral microfluidic biochip for the enrichment of circulating tumor cells | |
| Tan et al. | Microdevice for the isolation and enumeration of cancer cells from blood | |
| US20130337500A1 (en) | System and method for isolation of cells | |
| JP2016521350A (ja) | 規定された多細胞の組み合わせの分析のための方法および装置 | |
| US20160193606A1 (en) | Methods of and devices for capturing circulating tumor cells | |
| US11786900B2 (en) | Microfluidic device, microfluidic system and method for the isolation of particles | |
| CN210230001U (zh) | 微流道芯片以及微流道结构 | |
| CN210506297U (zh) | 微流道芯片以及微流道结构 | |
| Zhao | Sensitive and High-throughput Detection, Separation and Analysis of Circulating Tumor Cells |