SG11202112620TA - Phi29 dna polymerase mutants with improved primer recognition - Google Patents
Phi29 dna polymerase mutants with improved primer recognitionInfo
- Publication number
- SG11202112620TA SG11202112620TA SG11202112620TA SG11202112620TA SG11202112620TA SG 11202112620T A SG11202112620T A SG 11202112620TA SG 11202112620T A SG11202112620T A SG 11202112620TA SG 11202112620T A SG11202112620T A SG 11202112620TA SG 11202112620T A SG11202112620T A SG 11202112620TA
- Authority
- SG
- Singapore
- Prior art keywords
- lys
- phi29
- dna polymerase
- ile
- leu
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
- C12Y207/07—Nucleotidyltransferases (2.7.7)
- C12Y207/07007—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2521/00—Reaction characterised by the enzymatic activity
- C12Q2521/10—Nucleotidyl transfering
- C12Q2521/101—DNA polymerase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2533/00—Reactions characterised by the enzymatic reaction principle used
- C12Q2533/10—Reactions characterised by the enzymatic reaction principle used the purpose being to increase the length of an oligonucleotide strand
- C12Q2533/101—Primer extension
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201962849252P | 2019-05-17 | 2019-05-17 | |
| PCT/EP2020/063740 WO2020234200A1 (en) | 2019-05-17 | 2020-05-15 | Phi29 dna polymerase mutants with improved primer recognition |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SG11202112620TA true SG11202112620TA (en) | 2021-12-30 |
Family
ID=71016482
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG11202112620TA SG11202112620TA (en) | 2019-05-17 | 2020-05-15 | Phi29 dna polymerase mutants with improved primer recognition |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US20220235337A1 (https=) |
| EP (1) | EP3969575B1 (https=) |
| JP (2) | JP2022533366A (https=) |
| KR (1) | KR20220009977A (https=) |
| CN (1) | CN114174502B (https=) |
| AU (1) | AU2020277641B2 (https=) |
| CA (1) | CA3140244A1 (https=) |
| ES (1) | ES3015001T3 (https=) |
| SG (1) | SG11202112620TA (https=) |
| WO (1) | WO2020234200A1 (https=) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113122517B (zh) * | 2021-03-24 | 2023-02-14 | 深圳清华大学研究院 | 聚合酶突变体及其应用 |
| WO2024076991A2 (en) * | 2022-10-03 | 2024-04-11 | Singular Genomics Systems, Inc. | Modified enzymes and uses thereof |
| EP4665865A1 (en) | 2023-02-17 | 2025-12-24 | Anjarium Biosciences AG | Methods of making dna molecules and compositions and uses thereof |
| WO2025036438A1 (zh) | 2023-08-15 | 2025-02-20 | 上海金斯康生物科技有限公司 | 预测突变对蛋白酶活性的影响 |
| CN121343954A (zh) * | 2024-07-08 | 2026-01-16 | 中国科学院宁波材料技术与工程研究所 | 一种丧失外切酶活性的phi29 DNA聚合酶突变体及其制备方法和应用 |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5656493A (en) | 1985-03-28 | 1997-08-12 | The Perkin-Elmer Corporation | System for automated performance of the polymerase chain reaction |
| WO2009145820A2 (en) * | 2008-03-31 | 2009-12-03 | Pacific Biosciences Of California, Inc. | Generation of modified polymerases for improved accuracy in single molecule sequencing |
| ES2628739T3 (es) | 2009-10-15 | 2017-08-03 | Ibis Biosciences, Inc. | Amplificación por desplazamiento múltiple |
| KR101190792B1 (ko) * | 2010-08-16 | 2012-10-12 | 한국과학기술연구원 | 변형 뉴클레오티드 및 이를 이용한 실시간 중합효소 반응 |
| US9422535B2 (en) * | 2013-04-25 | 2016-08-23 | Thermo Fisher Scientific Baltics Uab | phi29 DNA polymerase mutants having increased thermostability and processivity |
| US9587263B2 (en) * | 2014-03-26 | 2017-03-07 | General Electric Company | Isothermal amplification under low salt condition |
| US10280411B2 (en) * | 2015-10-27 | 2019-05-07 | Pacific Biosciences of California, In.c | Methods, systems, and reagents for direct RNA sequencing |
| TWI788318B (zh) * | 2016-12-19 | 2023-01-01 | 美商寬騰矽公司 | 用於定序反應之聚合酶 |
| WO2019019222A1 (zh) * | 2017-07-28 | 2019-01-31 | 深圳华大智造科技有限公司 | 提高了热稳定性的Phi29 DNA聚合酶突变体及其应用 |
| CN112805372B (zh) * | 2018-10-11 | 2023-10-31 | 深圳华大生命科学研究院 | 热稳定性提升的Phi29 DNA聚合酶突变体及其在测序中的应用 |
-
2020
- 2020-05-15 EP EP20730981.6A patent/EP3969575B1/en active Active
- 2020-05-15 CA CA3140244A patent/CA3140244A1/en active Pending
- 2020-05-15 KR KR1020217040028A patent/KR20220009977A/ko active Pending
- 2020-05-15 SG SG11202112620TA patent/SG11202112620TA/en unknown
- 2020-05-15 US US17/611,861 patent/US20220235337A1/en active Pending
- 2020-05-15 JP JP2021568525A patent/JP2022533366A/ja active Pending
- 2020-05-15 AU AU2020277641A patent/AU2020277641B2/en active Active
- 2020-05-15 WO PCT/EP2020/063740 patent/WO2020234200A1/en not_active Ceased
- 2020-05-15 CN CN202080036838.XA patent/CN114174502B/zh active Active
- 2020-05-15 ES ES20730981T patent/ES3015001T3/es active Active
-
2025
- 2025-04-08 JP JP2025063479A patent/JP2025108504A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| CN114174502B (zh) | 2025-09-19 |
| JP2022533366A (ja) | 2022-07-22 |
| JP2025108504A (ja) | 2025-07-23 |
| CN114174502A (zh) | 2022-03-11 |
| AU2020277641A1 (en) | 2021-12-09 |
| EP3969575C0 (en) | 2025-01-01 |
| CA3140244A1 (en) | 2020-11-26 |
| KR20220009977A (ko) | 2022-01-25 |
| ES3015001T3 (en) | 2025-04-28 |
| EP3969575A1 (en) | 2022-03-23 |
| US20220235337A1 (en) | 2022-07-28 |
| WO2020234200A1 (en) | 2020-11-26 |
| EP3969575B1 (en) | 2025-01-01 |
| AU2020277641B2 (en) | 2026-02-19 |
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