KR20220009977A - 개선된 프라이머 인식을 갖는 phi29 dna 폴리머라아제 돌연변이체 - Google Patents

개선된 프라이머 인식을 갖는 phi29 dna 폴리머라아제 돌연변이체 Download PDF

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KR20220009977A
KR20220009977A KR1020217040028A KR20217040028A KR20220009977A KR 20220009977 A KR20220009977 A KR 20220009977A KR 1020217040028 A KR1020217040028 A KR 1020217040028A KR 20217040028 A KR20217040028 A KR 20217040028A KR 20220009977 A KR20220009977 A KR 20220009977A
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phi29
dna polymerase
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leu
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Korean (ko)
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다빌라 루이스 블랑코
세란테스 앙헬 호아킨 피체르
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포베이스바이오 에스엘
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    • C—CHEMISTRY; METALLURGY
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07007—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/101—DNA polymerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2533/00—Reactions characterised by the enzymatic reaction principle used
    • C12Q2533/10—Reactions characterised by the enzymatic reaction principle used the purpose being to increase the length of an oligonucleotide strand
    • C12Q2533/101—Primer extension

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  • Life Sciences & Earth Sciences (AREA)
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  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
KR1020217040028A 2019-05-17 2020-05-15 개선된 프라이머 인식을 갖는 phi29 dna 폴리머라아제 돌연변이체 Pending KR20220009977A (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962849252P 2019-05-17 2019-05-17
US62/849,252 2019-05-17
PCT/EP2020/063740 WO2020234200A1 (en) 2019-05-17 2020-05-15 Phi29 dna polymerase mutants with improved primer recognition

Publications (1)

Publication Number Publication Date
KR20220009977A true KR20220009977A (ko) 2022-01-25

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KR1020217040028A Pending KR20220009977A (ko) 2019-05-17 2020-05-15 개선된 프라이머 인식을 갖는 phi29 dna 폴리머라아제 돌연변이체

Country Status (10)

Country Link
US (1) US20220235337A1 (https=)
EP (1) EP3969575B1 (https=)
JP (2) JP2022533366A (https=)
KR (1) KR20220009977A (https=)
CN (1) CN114174502B (https=)
AU (1) AU2020277641B2 (https=)
CA (1) CA3140244A1 (https=)
ES (1) ES3015001T3 (https=)
SG (1) SG11202112620TA (https=)
WO (1) WO2020234200A1 (https=)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113122517B (zh) * 2021-03-24 2023-02-14 深圳清华大学研究院 聚合酶突变体及其应用
WO2024076991A2 (en) * 2022-10-03 2024-04-11 Singular Genomics Systems, Inc. Modified enzymes and uses thereof
EP4665865A1 (en) 2023-02-17 2025-12-24 Anjarium Biosciences AG Methods of making dna molecules and compositions and uses thereof
WO2025036438A1 (zh) 2023-08-15 2025-02-20 上海金斯康生物科技有限公司 预测突变对蛋白酶活性的影响
CN121343954A (zh) * 2024-07-08 2026-01-16 中国科学院宁波材料技术与工程研究所 一种丧失外切酶活性的phi29 DNA聚合酶突变体及其制备方法和应用

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5656493A (en) 1985-03-28 1997-08-12 The Perkin-Elmer Corporation System for automated performance of the polymerase chain reaction
WO2009145820A2 (en) * 2008-03-31 2009-12-03 Pacific Biosciences Of California, Inc. Generation of modified polymerases for improved accuracy in single molecule sequencing
ES2628739T3 (es) 2009-10-15 2017-08-03 Ibis Biosciences, Inc. Amplificación por desplazamiento múltiple
KR101190792B1 (ko) * 2010-08-16 2012-10-12 한국과학기술연구원 변형 뉴클레오티드 및 이를 이용한 실시간 중합효소 반응
US9422535B2 (en) * 2013-04-25 2016-08-23 Thermo Fisher Scientific Baltics Uab phi29 DNA polymerase mutants having increased thermostability and processivity
US9587263B2 (en) * 2014-03-26 2017-03-07 General Electric Company Isothermal amplification under low salt condition
US10280411B2 (en) * 2015-10-27 2019-05-07 Pacific Biosciences of California, In.c Methods, systems, and reagents for direct RNA sequencing
TWI788318B (zh) * 2016-12-19 2023-01-01 美商寬騰矽公司 用於定序反應之聚合酶
WO2019019222A1 (zh) * 2017-07-28 2019-01-31 深圳华大智造科技有限公司 提高了热稳定性的Phi29 DNA聚合酶突变体及其应用
CN112805372B (zh) * 2018-10-11 2023-10-31 深圳华大生命科学研究院 热稳定性提升的Phi29 DNA聚合酶突变体及其在测序中的应用

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CN114174502B (zh) 2025-09-19
JP2022533366A (ja) 2022-07-22
JP2025108504A (ja) 2025-07-23
CN114174502A (zh) 2022-03-11
AU2020277641A1 (en) 2021-12-09
EP3969575C0 (en) 2025-01-01
CA3140244A1 (en) 2020-11-26
ES3015001T3 (en) 2025-04-28
EP3969575A1 (en) 2022-03-23
US20220235337A1 (en) 2022-07-28
WO2020234200A1 (en) 2020-11-26
SG11202112620TA (en) 2021-12-30
EP3969575B1 (en) 2025-01-01
AU2020277641B2 (en) 2026-02-19

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